Fatigue monitoring device and colloidal gold application equipment box

Abstract

The invention discloses a device for fatigue monitoring and an equipment box applying colloidal gold, and belongs to the field of medical instruments, and the device comprises test paper prepared from colloidal gold and a fluorescent lamp. According to the device for fatigue monitoring and the colloidal gold equipment box, the fatigue degree of the human body can be rapidly detected, the operation threshold is extremely low, professional medical knowledge is not needed, and only two core operations (S1, blood drops are added to test paper; and S2, a fluorescent lamp irradiates to interpret the bar code), and the test strip adopts a visual fluorescent bar code design, so that the result interpretation is intuitive and easy to understand, and athletes, manual workers or common home users can quickly master the use method.

Description

Technical Field

[0001] This invention relates to a colloidal gold device box, and more particularly to a device and application of a colloidal gold device box for fatigue monitoring. Background Technology

[0002] In modern society, whether it's the high-intensity training of professional athletes, the continuous work of manual laborers, or the long-term energy depletion caused by work pressure and a fast-paced lifestyle for ordinary people, "fatigue" has become a common problem affecting physical health, work efficiency, and sports safety. However, current methods for assessing human fatigue still have significant limitations, making it difficult to achieve scientific, rapid, and accurate monitoring.

[0003] From the perspective of existing monitoring methods, the mainstream approaches can be divided into two categories: subjective assessment and traditional objective detection, but both have significant shortcomings. Subjective assessment mainly relies on personal subjective feelings (such as self-reported fatigue and drowsiness), the observer's experience judgment (such as observation of facial expressions after exercise), or fatigue assessment scales. This method is easily affected by subjective factors such as emotions, environment, and differences in individual tolerance, and cannot quantify the degree of fatigue. It is even more difficult to detect early latent fatigue—often only when the body has already experienced obvious discomfort, at which point the body may already be in a sub-healthy or potentially damaged state.

[0004] Traditional objective detection methods focus on physiological or biochemical indicators, such as indirectly reflecting fatigue through physiological signals like heart rate variability (HRV) and blood oxygen saturation, or assisting in judgment through laboratory tests of biochemical markers like creatine kinase (CK) and lactate (LACT). However, the former is greatly affected by exercise status and emotional fluctuations, and lacks specificity; the latter relies on large-scale testing equipment (such as biochemical analyzers) in professional medical institutions, with complex and time-consuming testing procedures (usually requiring several hours to several days to produce results), and high costs, failing to meet the need for "real-time monitoring"—especially in sports events, outdoor work scenarios, or daily health management, where people urgently need a convenient tool to quickly obtain fatigue status. Summary of the Invention

[0005] To address the aforementioned technical problems, this invention provides a device and application for fatigue monitoring, comprising a colloidal gold device box, test strips prepared using colloidal gold, and a fluorescent lamp.

[0006] Furthermore, the usage steps include:

[0007] S1: After drawing blood, drop the blood onto the test strip;

[0008] S2: Shine a fluorescent light on the test strip. If a barcode appears on the test strip, it indicates that the tumor necrosis factor-α (THF-α) level exceeds 10 picoseconds, thus quickly detecting whether the body is fatigued.

[0009] In summary, the present invention has the following advantages over the prior art:

[0010] Currently, there is a lack of fatigue monitoring devices that can be based on clear biochemical markers and take into account "speed, convenience and accuracy", which cannot meet the needs for real-time monitoring of fatigue status in different scenarios (sports, work, daily health).

[0011] The device and colloidal gold device box for fatigue monitoring provided by this invention can quickly detect the degree of fatigue in the human body. The operation threshold is extremely low, requiring no professional medical knowledge, and only two core operations (S1: adding blood droplets to the test strip; S2: interpreting the barcode under fluorescent light). Moreover, the test strip adopts a "visual fluorescent barcode" design, making the result interpretation intuitive and easy to understand. Whether it is an athlete, a manual laborer, or an ordinary home user, everyone can quickly master the method of use. Attached Figure Description

[0012] The accompanying drawings, which are included to provide a further understanding of the invention and form part of this invention, illustrate exemplary embodiments of the invention and are used to explain the invention, but do not constitute an undue limitation of the invention. In the drawings:

[0013] Figure 1 A diagram showing the relationship between indicators and fatigue damage;

[0014] Figure 2 The AUC curve;

[0015] Figure 3 TNF-α showed significantly high expression in the fatigue group;

[0016] Figure 4 The sensitivity curves are for TNF-α, IL-5, and MPO. Detailed Implementation

[0017] It should be noted that, unless otherwise specified, the embodiments and features described in the present invention can be combined with each other. The present invention will now be described in detail with reference to the accompanying drawings and embodiments.

[0018] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the exemplary embodiments of the present invention. As used herein, the singular form may also include the plural form unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0019] Unless otherwise specifically stated, the relative arrangement, numerical expressions, and values ​​of the components and steps set forth in these embodiments do not limit the scope of the invention. It should also be understood that, for ease of description, the dimensions of the various parts shown in the drawings are not drawn to actual scale. Techniques, methods, and devices known to those skilled in the art may not be discussed in detail, but where appropriate, such techniques, methods, and devices should be considered part of the specification. In all examples shown and discussed herein, any specific values ​​should be interpreted as merely exemplary and not as limitations. Therefore, other examples of exemplary embodiments may have different values. It should be noted that similar reference numerals and letters in the following figures denote similar items; therefore, once an item is defined in one figure, it need not be further discussed in subsequent figures.

[0020] See Figures 1 to 4 As shown, based on extensive previous basic experimental research, it has been found that human fatigue (especially exercise fatigue or chronic fatigue) is closely related to the activation of the immune system and the release of inflammatory factors. Among them, tumor necrosis factor-α (TNF-α), as a key pro-inflammatory cytokine, shows significant expression differences under fatigue conditions. When the body is in a state of fatigue or overexertion, TNF-α levels are significantly elevated, and its concentration changes are strongly correlated with the degree of fatigue. Simultaneously, cytokines such as granulocyte colony-stimulating factor (G-CSF), interferon-γ (IFN-γ), and the interleukin family (IL-10, IL-15, IL-5, IL-6), as well as indicators such as myeloperoxidase (MPO) and macrophage inflammatory protein-3α (MIP-3α), have also been confirmed to be associated with fatigue and potential physical damage. This invention is based on the above research, using TNF-α as the core detection indicator, and has developed a device and colloidal gold device box for fatigue monitoring.

[0021] This invention provides a device and application for fatigue monitoring, including a colloidal gold device box, test strips prepared using colloidal gold, and a fluorescent lamp.

[0022] Colloidal gold test strip: As the core component of the test, the test strip consists of a sample pad, a conjugate pad, a reaction membrane, an absorbent pad, and a PVC base plate. The conjugate pad is coated with colloidal gold particles (20-40 nm in size, ensuring specific binding efficiency) labeled with anti-human tumor necrosis factor-α (TNF-α) monoclonal antibody.

[0023] Fluorescent detection lamp: also known as a banknote detector lamp.

[0024] Supporting consumables: namely, disposable blood collection tubes.

[0025] The core principle of this device is as follows: utilizing the antigen-antibody specific binding reaction, when TNF-α in the blood sample to be tested binds to the colloidal gold-labeled antibody on the binding pad, a "colloidal gold-antibody-TNF-α" complex is formed, which moves towards the reaction membrane during chromatography; if the TNF-α concentration in the sample is ≥10 pg / mL (the threshold set according to the high expression data of TNF-α in the fatigue group in Figure 1), the complex will bind to the polyclonal antibody on the test strip, forming a visible fluorescent barcode.

[0026] The operation of this device is simple, requires no professional laboratory environment, can be completed by a single person, and takes ≤15 minutes in total. The specific steps are as follows:

[0027] S1: After drawing blood, drop the blood onto the test strip.

[0028] S2: Shine a fluorescent light on the test strip. If a barcode appears on the test strip, it indicates that the tumor necrosis factor alpha exceeds 10 picoseconds, thus quickly detecting whether the human body is fatigued.

[0029] Precautions:

[0030] (1) The test strips must be used within the shelf life and should be used immediately after opening to avoid moisture;

[0031] (2) The fingertips must be strictly disinfected during sample collection to avoid bacterial contamination affecting the test results;

[0032] (3) If the person being tested has recently had an infection or inflammatory disease (such as a cold or rheumatoid arthritis), it may lead to an increase in TNF-α. It is necessary to make a comprehensive judgment based on clinical symptoms to avoid misjudgment.

[0033] Application scenarios:

[0034] (1) Sports field: Used for monitoring athletes' training intensity to avoid sports injuries caused by overtraining;

[0035] (2) Industrial sector: Used for pre-employment fatigue screening of manual laborers (such as athletes, construction workers, and truck drivers) to reduce the risk of safety accidents;

[0036] (3) Daily health management: for the general population to conduct home testing, detect chronic fatigue in a timely manner, and assist in adjusting their work and rest.

[0037] Example 1: Operation Procedure

[0038] The operation process of this device has been optimized and requires no professional laboratory environment. It can be completed by a single person, and the entire process takes ≤15 minutes. The specific steps and key points of operation are as follows to ensure that operators can master them quickly and accurately:

[0039] (I) Preliminary preparation stage (takes 1-2 minutes)

[0040] 1. Remove the colloidal gold test strips, disposable blood collection tubes, and lancets from the packaging. Check that all consumables are within their expiration date and that the packaging is intact. Do not use if any items are expired or damaged.

[0041] 2. Connect the fluorescent detection lamp to the power supply and preheat for 1-2 minutes to ensure stable light intensity. At the same time, place it in a relatively dim environment (avoid direct strong light that may affect subsequent interpretation) at a distance of 5-10cm from the test strip to be tested, and adjust the illumination angle in advance.

[0042] 3. The operator should wash their hands thoroughly and disinfect the blood collection site (usually the fingertip) with 75% medical alcohol. The disinfection area should be centered on the blood collection point and have a diameter of ≥5cm. Allow the alcohol to evaporate naturally before performing the blood collection operation to avoid alcohol residue affecting the sample quality.

[0043] (II) Sample collection and loading stage (takes 2-3 minutes)

[0044] 1. Take out the disposable blood collection needle and follow the instructions to prick the disinfected fingertip skin. Discard the first drop of blood (the first drop of blood may contain tissue fluid, which may affect the accuracy of the test). Use a disposable blood collection tube to collect the subsequent blood samples. The collection volume should be controlled at 20-30μL to ensure that the sample volume meets the test requirements.

[0045] 2. Hold the blood collection tube and vertically drip 1-2 drops of blood sample (about 10-15μL) into the center of the sample pad of the colloidal gold test strip. Avoid dripping the sample outside the sample pad. After adding the sample, avoid touching the test strip to prevent sample contamination or test strip displacement.

[0046] (III) Response and Result Interpretation Stage (10-12 minutes)

[0047] 1. After adding the sample, place the test strip flat on a clean, level table, avoiding bending or tilting the test strip. Allow the sample to undergo natural chromatographic reaction under capillary action. The reaction time should be strictly controlled within 10 minutes. Do not shorten or extend the reaction time (too short a reaction time may lead to insufficient reaction and false negative results; too long a reaction time may result in nonspecific bands, interfering with the interpretation).

[0048] 2. After the reaction time is complete, turn on the preheated fluorescent detection lamp and point the light at the reaction membrane area of ​​the test strip. Observe whether a fluorescent barcode appears on the reaction membrane in a dimly lit environment. If the fluorescent barcode is clearly visible, it indicates that the TNF-α concentration in the sample is ≥10 pg / mL, suggesting that the human body is in a state of fatigue; if no fluorescent barcode appears, it indicates that the TNF-α concentration in the sample is <10 pg / mL, suggesting that the human body is not currently in a state of fatigue.

[0049] 3. After the results are interpreted, turn off the fluorescent detection light in time. In accordance with the medical waste disposal regulations, put the used test strips, blood collection tubes, blood collection needles and other consumables into a special medical waste collection bag for unified recycling and disposal to avoid environmental pollution or cross-infection.

[0050] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A device for fatigue monitoring and an application of a colloidal gold device box, characterized in that, This includes colloidal gold test kits, test strips prepared using colloidal gold, and fluorescent lamps.

2. The device for fatigue monitoring and the colloidal gold device box according to claim 1, characterized in that, The usage steps include: S1: After drawing blood, drop the blood onto the test strip; S2: Shine a fluorescent light on the test strip. If a barcode appears on the test strip, it indicates that the tumor necrosis factor-α exceeds 10 picograms, thus quickly detecting whether the human body is fatigued.

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