Citrus chachiensis hortorum extract with anti-oxidation effect as well as preparation method and application of citrus chachiensis hortorum extract

Abstract

The invention relates to the technical field of plant extract preparation, in particular to a citrus chachiensis hortorum extract with an anti-oxidation effect and a preparation method and application thereof.The preparation method comprises the following steps that citrus chachiensis hortorum powder and dipropylene glycol are mixed and homogenized for 20-30 min under the condition of 3500-4000 r / min, and a primary homogenized mixture is obtained; adding dipropylene glycol into the primarily homogenized mixture, and homogenizing for 20-30 minutes under the condition of 3500-4000 r / min to obtain a secondarily homogenized mixture; adding purified water into the secondarily homogenized mixture, and homogenizing for 30-35 minutes under the condition of 4000-4500 r / min to obtain a homogenized mixed solution; performing primary filtration, water bath extraction and secondary filtration on the homogeneous mixed solution to obtain a citrus chachiensis hortorum extract; the process is simple, the cost is low, the component dissolution rate is high, the antioxidant activity is strong, the batch is stable, and large-scale industrial production is easy to realize.

Description

Technical Field

[0001] This invention relates to the field of plant extract preparation technology, specifically to a Guangchenpi extract with antioxidant effects, its preparation method, and its application. Background Technology

[0002] Guangchenpi (Reticulatae Pericarpium) is the mature, dried peel of the Citrus reticulata plant (Citrus medica var. sarcodactylis), belonging to the Rutaceae family and the Citrus genus. It is processed through a specific aging process and is mainly produced in Xinhui District, Jiangmen City, Guangdong Province. It is a traditional Chinese medicine and food product. Modern research shows that the flavonoids in Guangchenpi, such as hesperidin, nobiletin, and tangeretin, are its core active ingredients, exhibiting significant antioxidant, anti-inflammatory, and metabolic-regulating effects. With the increasing demand for natural antioxidants, the preparation technology of Guangchenpi extract has received widespread attention. Currently, the main extraction methods for Guangchenpi extract include water extraction, alcohol extraction, single organic solvent extraction, and ultrasound / microwave-assisted extraction. However, all of these methods have significant drawbacks: water extraction has low extraction efficiency, and the flavonoids have poor water solubility, resulting in insufficient dissolution rates of core components such as hesperidin and nobiletin, and limited antioxidant activity of the extract; alcohol extraction can improve the dissolution of some components, but the risk of ethanol residue is high, limiting its application in food and cosmetics, and the production cost is also high; single organic solvent extraction methods (such as butylene glycol, isosorbide dimethyl ether, etc.) are not highly targeted and cannot simultaneously achieve the dissolution of flavonoids of different polarities, resulting in incomplete component extraction; ultrasound / microwave-assisted extraction can shorten the extraction time, but the equipment investment is large, the process is complex, large-scale production is difficult, and it is easy to cause degradation of some heat-sensitive components. For example, Chinese patent application (publication number CN118976066A) discloses a tangerine peel flower tea and its preparation method. The tangerine peel flower tea extract prepared by combining tangerine peel with licorice, wolfberry, red dates and chrysanthemum in a specific ratio and using water extraction method ensures the efficacy, but it still cannot effectively overcome the above defects of water extraction method.

[0003] Therefore, developing a simple, low-cost, high-dissolution-rate, highly antioxidant, and batch-stable method for preparing Guangchenpi extract has become an urgent technical problem to be solved in this field. Summary of the Invention

[0004] To address the aforementioned issues, this invention provides an extract of dried tangerine peel with antioxidant properties and its preparation method. The process is simple, low-cost, has a high component dissolution rate, strong antioxidant activity, and batch-to-batch stability, making it easy to achieve large-scale industrial production.

[0005] This invention provides a method for preparing a Guangchenpi extract with antioxidant effects, comprising the following steps: mixing Guangchenpi powder with dipropylene glycol and homogenizing at 3500-4000 r / min for 20-30 min to obtain a preliminary homogenized mixture; adding dipropylene glycol to the preliminary homogenized mixture and homogenizing at 3500-4000 r / min for 20-30 min to obtain a secondary homogenized mixture; adding purified water to the secondary homogenized mixture and homogenizing at 4000-4500 r / min for 30-35 min to obtain a homogenized liquid; and subjecting the homogenized liquid to primary filtration, water bath extraction, and secondary filtration to obtain the Guangchenpi extract.

[0006] In one embodiment, the Guangchenpi powder has an aging time of ≥3 years and a particle size of ≥90 mesh.

[0007] In one embodiment, the Guangchenpi powder is aged for 3-5 years and has a particle size of 100-120 mesh.

[0008] The preparation method of Guangchenpi powder is as follows: Select mature and dried peel of Chazhigan orange (aged for ≥3 years) from Xinhui District, Jiangmen City, Guangdong Province, remove impurities, dry, and pulverize to a particle size ≥ 90 mesh.

[0009] This invention optimizes the preparation process of Guangchenpi extract, specifically by using a propane glycol-purified water compound solvent system and a stepwise homogenization process, combined with controlled homogenization conditions, to achieve efficient dissolution of flavonoids such as hesperidin, norihesperidin, and tangeretin. The extract contains hesperidin ≥240 μg / mL, norihesperidin ≥105 μg / mL, and tangeretin ≥120 μg / mL, significantly enhancing the antioxidant activity of the extract.

[0010] Furthermore, this invention uses Guangchenpi powder aged for ≥3 years and with a particle size ≥90 mesh, combined with subsequent homogenization and extraction process conditions control, to balance dissolution efficiency and contact efficiency, allowing the solvent to quickly penetrate the cell wall and promote the dissolution of flavonoid components.

[0011] Furthermore, by sequentially subjecting the homogenized mixture after distribution homogenization to primary filtration, water bath extraction, and secondary filtration, the present invention further controls the water bath extraction temperature and filtration conditions, effectively avoiding the degradation of heat-sensitive components while ensuring extraction efficiency. The component content and antioxidant activity of the extract fluctuate little, the batch consistency is high, and it is easy to achieve large-scale production.

[0012] In one embodiment, the mass ratio of Guangchenpi powder to dipropylene glycol is 1:2.5-3.5, the mass ratio of the preliminary homogenized mixture to dipropylene glycol is 3.5-4.5:2.5-3.5, and the mass ratio of Guangchenpi powder to purified water is 1:1.8-2.2.

[0013] In one embodiment, the primary filtration includes the following steps: filtering the homogeneous mixture through a 400-500 mesh dual filter to remove solid impurities and obtain the primary filtrate.

[0014] In one embodiment, the filtration pressure of the dual filter is 0.3-0.5 MPa.

[0015] In one embodiment, the water bath extraction includes the following steps: placing the primary filtrate in a water bath at 100-110°C for 10-12 hours, and obtaining the supernatant after standing.

[0016] In one embodiment, the secondary filtration includes the following steps: filtering the supernatant through a 0.22 μm plate and frame filter, and collecting the filtrate, which is the Guangchenpi extract.

[0017] In one embodiment, the filtration flow rate of the plate and frame filter is 5-10 mL / min.

[0018] The process provided by this invention uses inexpensive and readily available raw materials with high utilization rates. It requires no auxiliary equipment such as ultrasound or microwave, and is simple and easy to operate, thus reducing the cost of large-scale production.

[0019] Another aspect of the present invention provides a tangerine peel extract with antioxidant effects, wherein the tangerine peel extract contains hesperidin ≥240 μg / mL, nobiletin ≥105 μg / mL, citrus terpene ≥120 μg / mL, DPPH free radical scavenging rate ≥85%, and ABTS free radical scavenging rate ≥90%.

[0020] The DPPH free radical scavenging rate of the tangerine peel extract provided by this invention is ≥85%, and the ABTS free radical scavenging rate is ≥90%. It has excellent antioxidant properties, can effectively remove free radicals in the body, and reduce oxidative stress damage. It can be widely used in antioxidant foods, health products, cosmetics and pharmaceuticals, and has broad market prospects.

[0021] The third aspect of this invention provides an application of a tangerine peel extract with antioxidant effects, which can be used in the preparation of antioxidant foods, health products, cosmetics or pharmaceuticals.

[0022] Beneficial effects 1. This invention provides an extract of dried tangerine peel with antioxidant effects and its preparation method. The process is simple, low-cost, has a high component dissolution rate, strong antioxidant activity, and batch stability, making it easy to achieve large-scale industrial production.

[0023] 2. This invention optimizes the preparation process of Guangchenpi extract, specifically by using a propane glycol-purified water compound solvent system and a stepwise homogenization process, combined with controlled homogenization conditions, to achieve efficient dissolution of flavonoids such as hesperidin, norihesperidin, and tangeretin. The extract contains hesperidin ≥240 μg / mL, norihesperidin ≥105 μg / mL, and tangeretin ≥120 μg / mL, significantly enhancing the antioxidant activity of the extract.

[0024] 3. This invention uses Guangchenpi powder aged for ≥3 years and with a particle size ≥90 mesh, combined with subsequent homogenization and extraction process conditions control, to balance dissolution efficiency and contact efficiency, allowing the solvent to quickly penetrate the cell wall and promote the dissolution of flavonoid components.

[0025] 4. This invention further controls the water bath extraction temperature and filtration conditions by sequentially performing primary filtration, water bath extraction, and secondary filtration on the homogenized mixture after distribution homogenization. This effectively avoids the degradation of heat-sensitive components while ensuring extraction efficiency. The component content and antioxidant activity of the extract fluctuate little, and the batch consistency is high, making it easy to achieve large-scale production.

[0026] 5. The process provided by this invention uses inexpensive and readily available raw materials with high utilization rates. It does not require auxiliary equipment such as ultrasound or microwave, and the process is simple and easy to operate, thus reducing the cost of large-scale production.

[0027] 6. The DPPH free radical scavenging rate of the tangerine peel extract provided by this invention is ≥85%, and the ABTS free radical scavenging rate is ≥90%. It has excellent antioxidant properties, can effectively remove free radicals in the body, reduce oxidative stress damage, and can be widely used in antioxidant foods, health products, cosmetics and pharmaceuticals. It has broad market prospects.

[0028] Example 1 Example 1 of the present invention provides an extract of dried tangerine peel with antioxidant effects. The preparation method of the extract includes the following steps: mixing dried tangerine peel powder with dipropylene glycol and homogenizing at 25°C and 4000 r / min for 20 min to obtain a preliminary homogenized mixture; adding dipropylene glycol to the preliminary homogenized mixture and homogenizing at 25°C and 4000 r / min for 20 min to obtain a secondary homogenized mixture; adding purified water to the secondary homogenized mixture and homogenizing at 25°C and 4000 r / min for 30 min to obtain a homogenized liquid; and subjecting the homogenized liquid to primary filtration, water bath extraction, and secondary filtration to obtain the dried tangerine peel extract.

[0029] The Guangchenpi powder has been aged for 3 years and has a particle size of 100 mesh. The preparation method of the Guangchenpi powder is as follows: select mature and dried peel of Chazhigan orange from Xinhui District, Jiangmen City, Guangdong Province (aged for 3 years), the raw material quality meets the requirements of the "Pharmacopoeia of the People's Republic of China" (2020 edition), remove impurities, dry at 60℃ for 2 hours, and pulverize to a particle size of 100 mesh.

[0030] The mass ratio of Guangchenpi powder to dipropylene glycol is 1:3, the mass ratio of the preliminary homogenized mixture to dipropylene glycol is 4:3, and the mass ratio of Guangchenpi powder to purified water is 1:2.

[0031] The primary filtration includes the following steps: filtering the homogeneous mixture through a 500-mesh dual filter to remove solid impurities and obtain the primary filtrate. The filtration pressure of the dual filter is 0.4 MPa.

[0032] The water bath extraction includes the following steps: placing the primary filtrate in a 100°C water bath for 10 hours and allowing it to stand to obtain the supernatant.

[0033] The secondary filtration includes the following steps: filtering the supernatant through a 0.22 μm plate and frame filter, collecting the filtrate, which is the Guangchenpi extract, and the filtration flow rate of the plate and frame filter is 8 mL / min.

[0034] Example 2 Example 2 of the present invention provides an extract of dried tangerine peel with antioxidant effects and its preparation method. The specific implementation method is the same as that of Example 1, except that the mass ratio of dried tangerine peel powder to dipropylene glycol is 1:2.5, the mass ratio of the preliminary homogenized mixture to dipropylene glycol is 3.5:2.5, and the mass ratio of dried tangerine peel powder to purified water is 1:2.2.

[0035] Example 3 Example 3 of the present invention provides an extract of dried tangerine peel with antioxidant effects and its preparation method. The specific implementation method is the same as in Example 1, except that the mass ratio of dried tangerine peel powder to dipropylene glycol is 1:3.5, the mass ratio of the preliminary homogenized mixture to dipropylene glycol is 4.5:3.5, and the mass ratio of dried tangerine peel powder to purified water is 1:1.8. In the preparation method of the extract of dried tangerine peel with antioxidant effects, the homogenized mixture is obtained by the following method: Guangchenpi powder was mixed with dipropylene glycol and homogenized at 25°C and 4000 r / min for 20 min to obtain a preliminary homogenized mixture. Dipropylene glycol was added to the preliminary homogenized mixture and homogenized at 25°C and 4000 r / min for 20 min to obtain a secondary homogenized mixture. Pure water was added to the secondary homogenized mixture and homogenized at 25°C and 4000 r / min for 35 min to obtain a homogenized liquid.

[0036] Comparative Example 1 Comparative Example 1 of the present invention provides an extract of dried tangerine peel with antioxidant effects and its preparation method. The specific implementation method is the same as that of Example 1, except that in the preparation method of the dried tangerine peel extract with antioxidant effects, the homogenized mixture is obtained by the following method: Guangchenpi powder and purified water were mixed at a mass ratio of 1:8 and homogenized at 25℃ and 4000 r / min for 70 min to obtain a homogenized mixture.

[0037] Comparative Example 2 Comparative Example 2 of the present invention provides an extract of dried tangerine peel with antioxidant effects and its preparation method. The specific implementation method is the same as that of Example 1, except that in the preparation method of the dried tangerine peel extract with antioxidant effects, the homogenized mixture is obtained by the following method: Guangchenpi powder and dipropylene glycol were mixed at a mass ratio of 1:8 and homogenized at 25℃ and 4000 r / min for 70 min to obtain a homogenized mixture.

[0038] Comparative Example 3 Comparative Example 3 of the present invention provides an extract of dried tangerine peel with antioxidant effects and its preparation method. The specific implementation method is the same as that of Example 1, except that the aging time of the dried tangerine peel powder is 2 years and the particle size is 100 mesh.

[0039] Comparative Example 4 Comparative Example 4 of the present invention provides an extract of dried tangerine peel with antioxidant effects and its preparation method. The specific implementation method is the same as that of Example 1, except that the dried tangerine peel powder is aged for 3 years and has a particle size of 80 mesh.

[0040] Comparative Example 5 Comparative Example 5 of the present invention provides an extract of dried tangerine peel with antioxidant effects and its preparation method. The specific implementation method is the same as that of Example 1, except that in the preparation method of the dried tangerine peel extract with antioxidant effects, the homogenized mixture is obtained by the following method: Guangchenpi powder, dipropylene glycol, and purified water were mixed at a mass ratio of 1:6:2 and homogenized at 25℃ and 4000 r / min for 70 min to obtain a homogenized mixture.

[0041] Comparative Example 6 Comparative Example 6 of the present invention provides an extract of dried tangerine peel with antioxidant effects and its preparation method. The specific implementation method is the same as that of Example 1, except that in the preparation method of the dried tangerine peel extract with antioxidant effects, the homogenized mixture is obtained by the following method: The powdered dried tangerine peel was mixed with dipropylene glycol and homogenized at 25°C and 3000 r / min for 20 min to obtain a preliminary homogenized mixture. Dipropylene glycol was added to the preliminary homogenized mixture and homogenized at 25°C and 3000 r / min for 20 min to obtain a secondary homogenized mixture. Pure water was added to the secondary homogenized mixture and homogenized at 25°C and 3000 r / min for 30 min to obtain a homogenized liquid.

[0042] Comparative Example 7 Comparative Example 7 of the present invention provides an extract of dried tangerine peel with antioxidant effects and its preparation method. The specific implementation method is the same as that of Example 1, except that the water bath extraction includes the following steps: placing the primary filtrate in a 90°C water bath for 10 hours and then allowing it to stand to obtain the supernatant.

[0043] Comparative Example 8 Comparative Example 8 of the present invention provides an extract of dried tangerine peel with antioxidant effects and its preparation method. The specific implementation method is the same as that of Example 1, except that the secondary filtration includes the following steps: filtering the supernatant through a 0.45μm plate and frame filter, collecting the filtrate, which is the dried tangerine peel extract. The filtration flow rate of the plate and frame filter is 8 mL / min.

[0044] Comparative Example 9 Comparative Example 9 of the present invention provides an extract of dried tangerine peel with antioxidant effects and its preparation method. The specific implementation method is the same as that of Example 1, except that dipropylene glycol is replaced with 1,3-butanediol.

[0045] Comparative Example 10 Comparative Example 10 of the present invention provides an extract of dried tangerine peel with antioxidant effects and its preparation method. The specific implementation method is the same as that of Example 1, except that in the preparation method of the dried tangerine peel extract with antioxidant effects, the homogenized mixture is obtained by the following method: The powdered dried tangerine peel was mixed with dipropylene glycol and isosorbide dimethyl ether and homogenized at 25°C and 4000 r / min for 20 min to obtain a preliminary homogenized mixture. Dipropylene glycol was added to the preliminary homogenized mixture and homogenized at 25°C and 4000 r / min for 20 min to obtain a secondary homogenized mixture. Pure water was added to the secondary homogenized mixture and homogenized at 25°C and 4000 r / min for 30 min to obtain a homogenized liquid.

[0046] The mass ratio of Guangchenpi powder to dipropylene glycol and isosorbide dimethyl ether is 1:2:1, the mass ratio of the preliminary homogenized mixture to dipropylene glycol is 4:3, and the mass ratio of Guangchenpi powder to purified water is 1:2.

[0047] Comparative Example 11 Comparative Example 11 of the present invention provides an extract of dried tangerine peel with antioxidant effects and its preparation method. The specific implementation method is the same as that of Example 1, except that the mass ratio of dried tangerine peel powder to dipropylene glycol is 1:2, the mass ratio of the preliminary homogenized mixture to dipropylene glycol is 3:2, and the mass ratio of dried tangerine peel powder to purified water is 1:3.

[0048] Comparative Example 12 Comparative Example 12 of the present invention provides an extract of Guangchenpi (dried tangerine peel) with antioxidant effects and its preparation method. The specific implementation method is the same as that of Example 1, except that the Guangchenpi powder is replaced with unaged Guangchenpi powder. The preparation method of the unaged Guangchenpi powder is as follows: select the mature fresh peel of Chazhigan (dried tangerine peel) from Xinhui District, Jiangmen City, Guangdong Province (unaged), remove impurities, dry at 60°C for 2 hours, and pulverize to a particle size of 100 mesh.

[0049] Application Example 1 Application Example 1 of the present invention provides an antioxidant oral liquid, which, by weight, comprises the following components: 10 parts of dried tangerine peel extract prepared in Example 1, 20 parts of honey, 0.5 parts of vitamin C, and 69.5 parts of purified water.

[0050] The preparation method of the antioxidant oral liquid is as follows: dissolve honey in purified water, add tangerine peel extract and vitamin C, stir evenly, sterilize and fill into bottles to make an oral liquid.

[0051] This oral liquid has a DPPH free radical scavenging rate of ≥80%, a sweet taste, and no odor, making it suitable for daily antioxidant health care.

[0052] Performance testing 1. Component content determination (HPLC-MS method): 1.1 Sample preparation: Take 1 mL of the tangerine peel extract prepared in each example and comparative example, add 9 mL of chromatographically pure methanol, filter through a 0.22 μm filter membrane and then load the sample; 1.2 Preparation of standard solutions: Using methanol as solvent, prepare standard solutions of hesperidin, nonotrimonin, tangeretin and naringin with a concentration of 100 μg / mL, and serially dilute to 0-10 μg / mL to plot standard curves; 1.3 Chromatographic conditions: The chromatographic column was an Eclipse plus C18 (2.1×50 mm, 1.8 μm); mobile phase A was water, and mobile phase B was methanol; column temperature was 40℃; injection volume was 1 μL; flow rate was 0.3 mL / min; elution gradient: 0-2 min, A 40%, B 60%; 2-4 min, A 40%→0%, B 60%→100%; 4-6 min, A 0%, B 100%; 6-6.1 min, A 0%→90%, B 100%→10%; 6.1-10 min, A 90%, B 10%. 1.4 Mass spectrometry conditions: MS QQQ Mass Spectrometer was used, with an Agilent JetStream ionization source; carrier gas temperature 300℃, carrier gas flow rate 8 L / min, nebulizer gas pressure 45 psi, sheath gas temperature 300℃, sheath gas flow rate 10 L / min, capillary voltage 4000 V (positive ion) / 3500 V (negative ion), nozzle voltage 1000 V (positive ion) / 500 V (negative ion); 1.5 Detection results: The contents of hesperidin, nonotrimonin, tangeretin and naringin in the sample were calculated according to the standard curve. The results are shown in Table 1.

[0053] 2. Antioxidant activity detection: 2.1 DPPH free radical scavenging rate detection: Take 0.1 mL of sample (each example and comparative example), add 3.9 mL of 0.1 mmol / L DPPH methanol solution, react in the dark for 30 min, and measure the absorbance at 517 nm (sample A); the blank group uses methanol instead of sample (blank A), and the control group uses methanol instead of DPPH solution (control A); scavenging rate = (1 - (sample A - control A) / blank A) × 100%, the results are shown in Table 1. 2.2 ABTS free radical scavenging rate detection: 7 mmol / L ABTS solution and 2.45 mmol / L potassium persulfate solution were mixed in equal volumes and allowed to stand in the dark for 12 h to prepare ABTS stock solution. The stock solution was diluted with methanol to an absorbance of 0.7 ± 0.02 (734 nm). 0.1 mL of sample (each example and comparative example) was taken and 3.9 mL of diluted ABTS solution was added. After reacting for 10 min, the absorbance was measured (sample A). The blank group was prepared by replacing the sample with methanol (blank A). Scavenging rate = (1 - sample A / blank A) × 100%. The results are shown in Table 1.

[0054] 2.3 Detection of ROS clearance rate in zebrafish embryos: 2.3.1 Test Grouping The test requires setting up a blank control group (fish embryo culture medium), a positive control group (glutathione working solution), and a test substance group (test substance - each example and comparative example). If an organic solvent is used to aid dissolution, the solvent concentration in each group solution must be the same. 2.3.1.1 Setting up a blank control group Twenty-four 2-day-old zebrafish embryos were randomly selected and placed into a 96-well plate, with each well containing one embryo and 0.2 mL of embryo culture medium. 2.3.1.2 Setting up a positive control group Twenty-four 2-day-old zebra embryos were randomly selected and placed into a 96-well plate, with each well containing one embryo and 0.2 mL of glutathione working solution. 2.3.1.3 Treatment of the test substance Twenty-four 2-day-old zebrafish embryos were randomly selected and placed into a 96-well plate, with each well containing one embryo and 0.2 mL of the test solution. Incubate at 28℃±1℃ for 24h±1h. Transfer to 24-well plates, each well containing 12 fish embryos and 2mL of reactive oxygen species (ROS) test solution, and incubate at 28℃±1℃ for another 2h±0.2h. 2.3.2 Microscopic analysis of the samples The fish embryos were placed on their sides. They were then photographed under a fluorescence stereomicroscope using standardized photographic parameters. 2.3.3 Data and Result Calculation Open the photo with the analysis software, mark the yolk region of each fish embryo, then select "Measure Average Intensity" in the "Measure" column, and select the "Average Signal Intensity" in the test results as the ROS signal intensity. Calculate the reactive oxygen species (ROS) scavenging rate: Scavenging rate = (B S) / B×100%(1) In formula (1): S—the average value of the ROS “mean signal intensity” of fish embryos in the test substance treatment group; B—the average value of the ROS “mean signal intensity” of fish embryos in the blank control group; Calculate the mean and standard error for each experimental group. Statistical results are expressed as mean ± standard error. Perform analysis of variance using statistical software. A two-tailed t-test is conducted to compare the mean ROS signal intensity between the test group and the blank control group (or solvent control group). p value, p <0.05 indicates a significant difference; see Table 1 for the results.

[0055] Table 1

[0056] As shown in Table 1, the tangerine peel extracts with antioxidant effects provided in Examples 1-3 have higher contents of hesperidin, nosenoside, citrus peel and naringin compared with Comparative Examples 1-12, and have better antioxidant effects.

Claims

1. A method for preparing a tangerine peel extract with antioxidant effects, characterized in that, Includes the following steps: Guangchenpi powder was mixed with dipropylene glycol and homogenized at 3500-4000 r / min for 20-30 min to obtain a preliminary homogenized mixture. Dipropylene glycol was added to the preliminary homogenized mixture and homogenized at 3500-4000 r / min for 20-30 min to obtain a secondary homogenized mixture. Purified water was added to the secondary homogenized mixture and homogenized at 4000-4500 r / min for 30-35 min to obtain a homogenized liquid. The homogenized liquid was subjected to primary filtration, water bath extraction, and secondary filtration to obtain Guangchenpi extract.

2. The method for preparing the tangerine peel extract with antioxidant effects according to claim 1, characterized in that, The Guangchenpi powder has an aging time of ≥3 years and a particle size of ≥90 mesh.

3. The method for preparing the tangerine peel extract with antioxidant effects according to claim 1, characterized in that, The mass ratio of Guangchenpi powder to dipropylene glycol is 1:2.5-3.5, the mass ratio of the preliminary homogenized mixture to dipropylene glycol is 3.5-4.5:2.5-3.5, and the mass ratio of Guangchenpi powder to purified water is 1:1.8-2.

2.

4. The method for preparing the tangerine peel extract with antioxidant effects according to claim 1, characterized in that, The primary filtration includes the following steps: filtering the homogeneous mixture through a 400-500 mesh dual filter to remove solid impurities and obtain the primary filtrate.

5. The method for preparing the tangerine peel extract with antioxidant effects according to claim 4, characterized in that, The filtration pressure of the dual filter is 0.3-0.5 MPa.

6. The method for preparing the tangerine peel extract with antioxidant effects according to claim 4, characterized in that, The water bath extraction includes the following steps: placing the primary filtrate in a water bath at 100-110℃ for 10-12 hours, and then taking the supernatant after standing.

7. The method for preparing the tangerine peel extract with antioxidant effects according to claim 6, characterized in that, The secondary filtration includes the following steps: filtering the supernatant through a 0.22 μm plate and frame filter, and collecting the filtrate, which is the Guangchenpi extract.

8. The method for preparing the tangerine peel extract with antioxidant effects according to claim 7, characterized in that, The filtration flow rate of the plate and frame filter is 5-10 mL / min.

9. An extract of dried tangerine peel with antioxidant properties, prepared by the preparation method according to any one of claims 1-8, characterized in that, The extract of dried tangerine peel contains hesperidin at a content ≥240 μg / mL, nosenoside at a content ≥105 μg / mL, and citrus peel at a content ≥120 μg / mL.

10. An application of the tangerine peel extract with antioxidant effects according to claim 9, characterized in that, It can be used in the preparation of antioxidant foods, health products, cosmetics, or pharmaceuticals.

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