Sampling interface device

Abstract

The invention discloses a sampling interface device, and relates to the technical field of biological detection. The sampling interface device comprises a sampling interface main body, a fuel adding assembly and an ignition assembly, the sampling connector body is suitable for being in butt joint with a sampling opening of a cell culture container and provided with a sampling channel and a combustion chamber, the sampling channel is used for allowing a sampling instrument to pass through so as to enter the cell culture container, and the combustion chamber is provided with a flame outlet close to the sampling channel. The fuel adding assembly communicates with the combustion chamber and is used for conveying fuel to the combustion chamber. The ignition assembly is arranged inside or outside the sampling connector body and used for igniting fuel in the combustion chamber so as to sterilize and disinfect the sampling channel. The invention provides a wave type bioreactor paper carrier sampling interface device, which realizes sterile sampling of a carrier in a bioreactor cell culture process, and is convenient and effective to operate.

Description

Technical Field

[0001] This invention relates to the field of biological detection technology, and in particular to a sampling interface device. Background Technology

[0002] A wave-type bioreactor is a technological device that achieves cell culture through non-invasive wave-like oscillation motion. Cell culture containers typically use cell culture bags, and in the process of adhering cell culture, polyester fiber paper carriers are currently commonly used as cell adsorption carriers.

[0003] Currently, bioreactors are generally placed in a Class C clean environment. During the culture process, it is necessary to sample the paper carrier to observe cell adhesion and growth. However, direct open operation may lead to the risk of bacterial contamination. The commonly used sampling methods are to transfer the cell culture bag to a biosafety cabinet for sampling or to take samples through a sampling interface device.

[0004] However, when using sampling tools to sterilize in a biosafety cabinet, it is still necessary to repeatedly reach into the bag to grab the paper carrier during sampling. The surface of the tool may be contaminated with non-sterile areas at the edge of the bag opening (sampling port). Furthermore, the longer the tool is exposed in the cabinet, the higher the risk of microbial adhesion due to contact with air. Summary of the Invention

[0005] The main objective of this invention is to provide a sampling interface device that aims to reduce the risk of microbial adhesion during carrier sampling in bioreactor cell culture and achieve aseptic sampling.

[0006] To achieve the above objectives, the present invention provides a sampling interface device, comprising: The sampling interface body is adapted to dock with the sampling port of the cell culture container. The sampling interface body is provided with a sampling channel and a combustion chamber. The sampling channel is used to allow sampling instruments to pass through and enter the cell culture container. The combustion chamber has a flame outlet located near the sampling channel. A fuel addition assembly, in communication with the combustion chamber and for supplying fuel to the combustion chamber; and An ignition assembly, located inside or outside the sampling interface body, is used to ignite the fuel in the combustion chamber to sterilize and disinfect the sampling channel.

[0007] Optionally, the sampling interface body includes a liquid storage chamber and a sealing cap. An avoidance channel is provided through the middle of the liquid storage chamber. The combustion chamber is located at the periphery of the liquid storage chamber and surrounds the avoidance channel. The sealing cap is detachably installed at the entrance and exit of the sampling channel.

[0008] Optionally, the liquid storage chamber is made of stainless steel; and / or The inner wall polishing degree of the liquid storage cavity is less than or equal to 0.4 μm; and / or The volume of the liquid storage chamber is 5-10 mL.

[0009] Optionally, the flame outlet is located on the side of the combustion chamber facing the sealing cover.

[0010] Optionally, the sampling interface body further includes a heat-insulating connector, which is located on the side of the liquid storage cavity away from the sealing cap and is used to block the heat of the liquid storage cavity from being transferred to the cell culture container. One end of the heat-insulating connector is embedded in the clearance channel and threadedly connected to the sealing cap. The other end of the heat-insulating connector has an interface suitable for docking with the sampling port of the cell culture container. The middle part of the heat-insulating connector forms the sampling channel communicating with the interface.

[0011] Optionally, the heat-insulating connector includes a connecting section, a heat-insulating section, and a sealing section connected in sequence. The connecting section has an external thread that is threaded to the sealing cap, and the end face of the connecting section has a first sealing gasket for sealing the connection between the connecting section and the sealing cap. The heat-insulating section at least partially encloses the liquid storage cavity. The sealing section has the mating interface and an annular groove located on the inner end face of the mating interface. The annular groove has a second sealing gasket for sealing the connection between the end face of the sealing section and the cell culture container.

[0012] Optionally, the heat-insulating connector is made of ceramic material, and the connecting section, the heat-insulating section, and the sealing section are integrally formed; and / or The length of the insulation section is 15-20cm.

[0013] Optionally, the fuel filling assembly includes a fuel filling port, a guide pipe, and a switch valve. The fuel filling port is covered with a sealing cap, and the fuel filling port is connected to the combustion chamber through the guide pipe. The switch valve is located on the guide pipe.

[0014] Optionally, the ignition assembly includes an igniter, an ignition controller, and a power supply. The power supply is electrically connected to the ignition controller, and the ignition controller is connected to the igniter via a wire. Alternatively, the igniter includes an ignition body and an ignition power supply electrically connected to the ignition body. The ignition power supply is wirelessly connected to the ignition controller, and the igniter is mounted on the sampling interface body.

[0015] Optionally, the igniter is a high-voltage electric spark igniter, the igniter has an electrode pair, the electrode spacing of the electrode pair is 1-2mm, the ignition voltage is 10-15kV, and the frequency is 1-3 times / second.

[0016] In the technical solution of this invention, the sampling interface device includes a sampling interface body, a fuel addition component, and an ignition component. The sampling interface body is adapted to dock with the sampling port of a cell culture container. The sampling interface body is provided with a sampling channel and a combustion chamber. The sampling channel allows sampling instruments to pass through and enter the cell culture container. The combustion chamber has a flame outlet located near the sampling channel. The fuel addition component communicates with the combustion chamber and is used to supply fuel to the combustion chamber. The ignition component is located inside or outside the sampling interface body and is used to ignite the fuel in the combustion chamber to sterilize the sampling channel. It can be understood that this invention provides a sampling interface device for a wave-shaped bioreactor paper carrier. By setting the sampling interface body to form a sampling channel as a new sampling port for the cell culture container, and by setting the fuel addition component and the ignition component, the sampling channel can be sterilized at high temperature during sampling, effectively reducing the risk of microorganisms adhering to the sampling port during carrier sampling operations in the bioreactor cell culture process, achieving aseptic sampling, and ensuring the viability of the sampled cells. Furthermore, the sampling operation is convenient and effective by using this sampling interface device. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without creative effort.

[0018] Figure 1 This is a schematic diagram of the structure of an embodiment of the sampling interface device of the present invention. Figure 1 ; Figure 2 This is a schematic diagram of the structure of an embodiment of the sampling interface device of the present invention. Figure 2 ; Figure 3 This is an exploded view of an embodiment of the sampling interface device of the present invention.

[0019] Explanation of icon numbers: 10. Sampling interface body; 20. Fuel addition assembly; 30. Ignition assembly; 10a. Sampling channel; 10b. Combustion chamber; 10b1. Flame outlet; 11. Liquid storage chamber; 12. Sealing cap; 13. Heat insulation connector; 10a1. Connecting interface; 131. Connecting section; 132. Heat insulation section; 133. Sealing section; 14. First sealing gasket; 15. Second sealing gasket; 21. Fuel addition port; 22. Guide tube; 23. Switch valve; 24. Sealing cap; 31. Ignition device; 32. Ignition controller and power supply.

[0020] The realization of the objective, functional features and advantages of the present invention will be further explained in conjunction with the embodiments and with reference to the accompanying drawings. Detailed Implementation

[0021] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of them. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0022] It should be noted that all directional indications (such as up, down, left, right, front, back, etc.) in the embodiments of the present invention are only used to explain the relative positional relationship and movement of each component in a certain specific posture (as shown in the figure). If the specific posture changes, the directional indication will also change accordingly.

[0023] In the description of this invention, it should also be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0024] Furthermore, the use of terms such as "first" and "second" in this invention is for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined with "first" or "second" may explicitly or implicitly include at least one of those features. The word "and / or" throughout the text means including three parallel solutions; for example, "A and / or B" includes solution A, solution B, or a solution that simultaneously satisfies A and B. The technical solutions of the various embodiments can be combined with each other, but this must be based on the ability of those skilled in the art to implement them. When the combination of technical solutions is contradictory or impossible to implement, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection claimed by this invention.

[0025] This invention proposes a sampling interface device suitable for use in 3D large-scale adherent cell culture in bioreactors that can provide a three-dimensional growth matrix or surface for cells, such as glass bioreactors (fixed bed type) and wave bioreactors. The sampling interface device is installed at the sampling port of the bioreactor as a sampling interface. The shape of the interface of the sampling interface device and the position of the sampling port inside it can be adaptively adjusted according to the sampling port of the bioreactor, which is not limited here.

[0026] Reference Figures 1 to 3 In one embodiment of the present invention, the sampling interface device includes a sampling interface body 10, a fuel addition component 20, and an ignition component 30. The sampling interface body 10 is adapted to dock with the sampling port of a cell culture container. The sampling interface body 10 is provided with a sampling channel 10a and a combustion chamber 10b. The sampling channel 10a is used to allow sampling instruments to pass through and enter the cell culture container. The combustion chamber 10b has a flame outlet 10b1 located near the sampling channel 10a. The fuel addition component 20 is connected to the combustion chamber 10b and is used to deliver fuel to the combustion chamber 10b. The ignition component 30 is located inside or outside the sampling interface body 10 and is used to ignite the fuel in the combustion chamber 10b to sterilize the sampling channel 10a.

[0027] In this embodiment, the sampling interface body 10 may include several housings, and the housings are made of high temperature resistant materials. The specific structure of the sampling interface body 10 is not limited here.

[0028] The fuel addition assembly 20 may be configured to deliver liquid fuel, gaseous fuel, or a mixture of gas and liquid fuel to the combustion chamber 10b. The fuel is preferably alcohol, but this is not a limitation.

[0029] The ignition assembly 30 may be a manual or automatic ignition device such as an electric spark igniter, resistance wire igniter, flint igniter, piezoelectric igniter or lighter that can ignite liquid fuel, gaseous fuel or gas-liquid mixture, and there is no limitation to this.

[0030] It is understood that this invention provides a sampling interface device for a wave-shaped bioreactor paper carrier. By setting the sampling interface body 10 to form a sampling channel 10a as a new sampling port for the cell culture container, and by setting the fuel addition component 20 and the ignition component 30, the sampling channel 10a can be sterilized at high temperature during sampling. This effectively reduces the risk of microorganisms adhering to the sampling port during cell culture in the bioreactor, achieving aseptic sampling and ensuring the viability of the sampled cells. Furthermore, using this sampling interface device makes the sampling operation convenient and effective.

[0031] In one embodiment, reference is made to Figures 1 to 3 The sampling interface body 10 may include a liquid storage chamber 11 and a sealing cover 12. A clearance channel is provided through the middle of the liquid storage chamber 11. The combustion chamber 10b is located at the periphery of the liquid storage chamber 11 and is arranged around the clearance channel to form a ring flame, thereby improving the uniformity of heating the sampling channel 10a. The sealing cover 12 is detachably installed at the entrance and exit of the sampling channel 10a.

[0032] To improve the uniformity of high-temperature sterilization of the sampling channel 10a and to enhance the convenience and safety of operation, in this embodiment, preferably, the flame outlet 10b1 can be opened on the side of the combustion chamber 10b facing the sealing cover 12, and the flame outlet 10b1 is arranged in a ring.

[0033] In this embodiment, the inner diameter of the liquid storage chamber 11 is adapted to the size of the inlet and outlet of the sampling channel 10a. The inner diameter can be set to be greater than 20 mm, but is not limited here. When the alcohol burns to form a ring flame, sampling instruments such as tweezers can pass through smoothly, ensuring the sterility of the sampling process and the viability of the sampled cells.

[0034] In this embodiment, the liquid storage chamber 11 is made of stainless steel, preferably 316L stainless steel; the inner wall polishing degree of the liquid storage chamber 11 is less than or equal to 0.4μm (roughness Ra≤0.4μm); the volume of the liquid storage chamber 11 is 5-10mL, which is suitable for the storage requirements of conventional alcohol (concentration 95%).

[0035] In one embodiment, reference is made to Figures 1 to 3 The sampling interface body 10 may also include a heat-insulating connector 13. The heat-insulating connector 13 is located on the side of the liquid storage chamber 11 away from the sealing cap 12 and is used to prevent heat transfer from the liquid storage chamber 11 to the cell culture container. One end of the heat-insulating connector 13 is embedded in the clearance channel and threadedly connected to the sealing cap 12. The other end of the heat-insulating connector 13 has an interface 10a1 suitable for docking with the sampling port of the cell culture container. The middle of the heat-insulating connector 13 forms a sampling channel 10a that communicates with the interface 10a1. In this way, the threaded connection effectively ensures the sealing performance, further improves the convenience and safety of the sampling operation, and effectively ensures that heat is transferred to the cell culture container, thus preventing it from affecting the accuracy of the test results or even damaging the cell growth environment.

[0036] In this embodiment, refer to Figure 2 and Figure 3 The heat-insulating connector 13 may include a connecting section 131, a heat-insulating section 132, and a sealing section 133 connected in sequence. The connecting section 131 has an external thread that connects to the sealing cap 12, and a first sealing gasket 14 for sealing the connection between the connecting section 131 and the sealing cap 12 is provided on the end face of the connecting section 131. The heat-insulating section 132 at least covers part of the liquid storage cavity 11. The sealing section 133 has a mating interface 10a1 and an annular groove located on the inner end face of the mating interface 10a1. A second sealing gasket 15 for sealing the connection between the end face of the sealing section 133 and the cell culture container is provided in the annular groove. This configuration can improve the sealing performance of the sampling interface device and effectively prevent cell culture leakage.

[0037] In this embodiment, the connecting section 131, the heat insulation section 132, and the sealing section 133 can be integrally molded to ensure the overall airtightness and reliability of the heat insulation connector 13. The length of the heat insulation section 132 can be set to 15-20cm to increase the heat transfer path. The connecting section 131 can adopt an M20×1.5 thread structure. The mating interface 10a1 of the sealing section 133 matches the sampling interface of the culture bag.

[0038] In this embodiment, the heat-insulating connector 13 is integrally disposed between the sampling interface body 10 and the corrugated cell culture bag to achieve a sealed connection between the two and block heat transfer. The heat-insulating connector 13 can be made of high-purity alumina ceramic (purity ≥99%), which has a low thermal conductivity (thermal conductivity ≤20W / (m²) at 20℃). K)), with excellent high-temperature resistance (maximum operating temperature ≥1600℃), can effectively block the heat generated during sampling from being transferred to the culture container.

[0039] In this embodiment, both the first sealing gasket 14 and the second sealing gasket 15 can be made of food-grade silicone rubber with a Shore hardness of 50-60HA.

[0040] To improve the convenience and safety of refueling, in one embodiment, refer to Figures 1 to 3 The fuel filling assembly 20 may include a fuel filling port 21, a guide pipe 22 and a switch valve 23. The fuel filling port 21 is covered with a sealing cap 24. The fuel filling port 21 is connected to the combustion chamber 10b through the guide pipe 22. One end of the guide pipe 22 is connected to the top of the liquid storage chamber 11. The switch valve 23 is located on the guide pipe 22.

[0041] The fuel filling port 21 can adopt a funnel-like structure or a hollow pipe with an inner diameter of 6-12mm for easy and quick alcohol replenishment. The sealing cap 24 can be equipped with a silicone sealing ring to ensure the sealing of the liquid storage chamber and prevent alcohol evaporation. The diameter of the guide tube 22 can be 1-5mm. The guide tube 22 can be set at an angle, facing the annular combustion chamber 10b outside the sampling channel 10a of the sampling interface body 10, so as to accurately guide the alcohol into the combustion chamber 10b. In this embodiment, alcohol can be added by controlling the opening and closing of the switch valve 23.

[0042] In one embodiment, reference is made to Figures 1 to 3 The ignition assembly 30 may include an igniter 31, an ignition controller, and a power supply 32. The power supply is electrically connected to the ignition controller, and the ignition controller is wired or wirelessly connected to the igniter 31. The igniter 31 is mounted on the sampling interface body 10. This enables automatic ignition, which helps to further improve the efficiency and safety of the sampling operation.

[0043] Specifically, the ignition controller and the igniter 31 can be connected by a wire; or, the igniter 31 includes an ignition body and an ignition power supply electrically connected to the ignition body, and the ignition power supply and the ignition controller can achieve wireless power transmission by setting a transmitting coil and a receiving coil.

[0044] In this embodiment, the igniter 31 is a high-voltage electric spark igniter 31. The igniter 31 has an electrode pair. The ignition electrode pair can be made of high-temperature resistant alloy materials such as nickel-chromium alloy. The electrode spacing of the electrode pair can be 1-2mm. The ignition voltage can be 10-15kV and the frequency can be 1-3 times / second to ensure that the alcohol can be reliably ignited.

[0045] In this embodiment, the ignition controller can be integrated into the intelligent control unit of the bioreactor, supporting the setting of ignition parameters (such as ignition time, ignition duration, extinguishing time, etc.) via a touch screen or remote terminal. For example, setting "ignite for disinfection for 30 seconds before sampling, and automatically extinguish after disinfection" ensures precise coordination between alcohol and ignition action.

[0046] The power supply provides a stable power supply to the ignition assembly 30. It can be a 5000mAh lithium battery with a DC12V voltage and supports charging. A single charge can meet the needs of 100-200 ignition operations.

[0047] When the wave-shaped bioreactor paper carrier sampling interface device of the present invention is in operation, the following steps can be followed: (1) Preparation stage Before the culture phase begins, connect the lower sealing section 133 of the irradiated sterilized heat-insulated connector 13 to the sampling port of the corrugated cell culture bag in the biosafety cabinet to ensure a good seal. Before sampling begins, inject 75% medical alcohol into the combustion chamber 10b through the fuel filling port 21 of the fuel filling assembly 20 until it reaches 80% of the reservoir volume (do not add too much to avoid overflow from the flame outlet 10b1).

[0048] (2) Disinfection stage The disinfection program of the control system is started. After the set time is reached, the switch valve 23 of the fuel addition component 20 is opened to add fuel into the combustion chamber 10b. Subsequently, under the control of the program, the igniter 31 of the ignition component 30 is started to generate a high-voltage electric spark, ignite the fuel, and disinfect the outer wall of the sampling channel 10a with flame.

[0049] (3) Sampling stage Open the sealing cover 12 above the sampling interface device. At this time, the flame is burning. Then use medical disposable tweezers to perform the paper carrier sampling operation.

[0050] (4) Sampling completion stage After sampling is completed, cover the sealing cap 12, reseal the inlet and outlet of the sampling channel 10a, close the switch valve 23 of the fuel addition component 20, and turn off the igniter 31. At this time, the flame is extinguished.

[0051] In summary, the sampling interface device of the present invention adopts an annular liquid storage chamber 11 and a fuel addition component 20, which realizes convenient storage and addition of fuel. With the help of the program-controlled ignition component 30, manual operation is reduced, the degree of automation is high, and the safety hazards of manual ignition are avoided, thus improving the sterility of the sampling process.

[0052] In this invention, the heat-insulating connector 13 of the sampling interface device is made of high-purity alumina ceramic material, which has low thermal conductivity and high temperature resistance. It effectively blocks the heat generated during the sampling process from being transferred to the cell culture container, preventing the cell culture container from being damaged by heat, ensuring the integrity and safety of the culture system, and solving the heat conduction problem of metal or plastic connecting components.

[0053] During batch culture, the sampling interface device of the present invention can perform multiple samplings. The sampling process can be linked with the control system of the wave bioreactor. The system can control the electric spark generator to automatically ignite and set the sampling program to meet the continuous sampling requirements in industrial production.

[0054] The technical solution of the present invention is applicable to disposable bioreactors, especially sampling operations of flexible bags. It can be repeated multiple times, is easy to operate, and allows for aseptic in-situ sampling without transferring cell culture bags or disrupting the dynamic culture environment inside the wave-shaped bioreactor.

[0055] The above description is merely an optional embodiment of the present invention and does not limit the patent scope of the present invention. All equivalent structural transformations made using the contents of the present invention's specification and drawings under the inventive concept of the present invention, or direct / indirect applications in other related technical fields, are included within the patent protection scope of the present invention.

Claims

1. A sampling interface device, characterized in that, include: The sampling interface body is adapted to dock with the sampling port of the cell culture container. The sampling interface body is provided with a sampling channel and a combustion chamber. The sampling channel is used to allow sampling instruments to pass through and enter the cell culture container. The combustion chamber has a flame outlet located near the sampling channel. A fuel addition assembly, connected to the combustion chamber and used to deliver fuel to the combustion chamber; as well as An ignition assembly, located inside or outside the sampling interface body, is used to ignite the fuel in the combustion chamber to sterilize and disinfect the sampling channel.

2. The sampling interface device as described in claim 1, characterized in that, The sampling interface body includes a liquid storage chamber and a sealing cap. A clearance channel is provided through the middle of the liquid storage chamber. The combustion chamber is located at the periphery of the liquid storage chamber and surrounds the clearance channel. The sealing cap is detachably installed at the entrance and exit of the sampling channel.

3. The sampling interface device as described in claim 2, characterized in that, The liquid storage chamber is made of stainless steel; and / or The inner wall polishing degree of the liquid storage cavity is less than or equal to 0.4 μm; and / or The volume of the liquid storage chamber is 5-10 mL.

4. The sampling interface device as described in claim 2, characterized in that, The flame outlet is located on the side of the combustion chamber facing the sealing cover.

5. The sampling interface device as described in claim 2, characterized in that, The sampling interface body also includes a heat-insulating connector. The heat-insulating connector is located on the side of the liquid storage cavity away from the sealing cap and is used to block the heat of the liquid storage cavity from being transferred to the cell culture container. One end of the heat-insulating connector is embedded in the clearance channel and threadedly connected to the sealing cap. The other end of the heat-insulating connector has an interface suitable for docking with the sampling port of the cell culture container. The middle part of the heat-insulating connector forms the sampling channel that communicates with the interface.

6. The sampling interface device as described in claim 5, characterized in that, The heat-insulating connector includes a connecting section, a heat-insulating section, and a sealing section connected in sequence. The connecting section has an external thread that is threaded to the sealing cap, and a first sealing gasket is provided on the end face of the connecting section for sealing the connection between the connecting section and the sealing cap. The heat-insulating section at least partially encloses the liquid storage cavity. The sealing section has the mating interface and an annular groove located on the inner end face of the mating interface. A second sealing gasket is provided in the annular groove for sealing the connection between the end face of the sealing section and the cell culture container.

7. The sampling interface device as described in claim 6, characterized in that, The heat-insulating connector is made of ceramic material, and the connecting section, the heat-insulating section, and the sealing section are integrally formed; and / or The length of the insulation section is 15-20cm.

8. The sampling interface device as described in claim 1, characterized in that, The fuel filling assembly includes a fuel filling port, a guide pipe, and a switch valve. The fuel filling port is covered with a sealing cap. The fuel filling port is connected to the combustion chamber through the guide pipe. The switch valve is located on the guide pipe.

9. The sampling interface device as described in claim 1, characterized in that, The ignition assembly includes an igniter, an ignition controller, and a power supply. The power supply is electrically connected to the ignition controller, and the ignition controller is connected to the igniter via a wire. Alternatively, the igniter includes an ignition body and an ignition power supply electrically connected to the ignition body. The ignition power supply is wirelessly connected to the ignition controller, and the igniter is mounted on the sampling interface body.

10. The sampling interface device as described in claim 9, characterized in that, The igniter is a high-voltage electric spark igniter, which has an electrode pair with an electrode spacing of 1-2 mm, an ignition voltage of 10-15 kV, and a frequency of 1-3 times / second.

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