Bionic healing composite abutment of tissue engineering scaffold structure

Abstract

The invention discloses a bionic healing composite abutment of a tissue engineering scaffold structure, and relates to the field of bionic healing abutments.The bionic healing composite abutment comprises an abutment body, a threaded connecting part, a conical gum penetrating part and a shaping end, the threaded connecting part, the conical gum penetrating part and the shaping end are sequentially arranged on the abutment body from bottom to top, and the conical gum penetrating part and the shaping end are connected through a connecting mechanism; a butt joint platform is arranged at the end of the side, close to the shaping end, of the conical gum penetrating part, and a threaded short column is arranged on the surface of the butt joint platform. According to the bionic healing composite abutment of the tissue engineering scaffold structure, the shaping end part and the conical gingival penetrating part are detachably arranged through the connecting mechanism, so that the shaping end part can be independently taken down and replaced when the subsequent gingival cuff part is red, swollen and inflamed, and the whole healing abutment does not need to be replaced; therefore, the stability of the implant is not easily influenced, and the implant-bone initial joint surface is not damaged, so that the tooth implantation work of a patient is facilitated.

Description

Technical Field

[0001] This invention relates to biomimetic healing abutment technology, specifically to a biomimetic healing composite abutment with a tissue engineering scaffold structure. Background Technology

[0002] A biomimetic healing abutment is a material or structure used in the medical and bioengineering fields to mimic the healing process of organisms in nature, thereby promoting tissue regeneration and repair. In the field of dental implantology, biomimetic healing abutments are crucial for promoting osseointegration and improving implant success rates.

[0003] Healing abutments are typically made of titanium alloy and consist of a threaded connection at the bottom, a tapered transgingival portion in the middle, and a molded end at the top. They are usually designed as a single piece for easy assembly and disassembly. However, in practice, when inflammation and swelling occur between the molded end of the healing abutment and the gum due to bacterial growth or other reasons, requiring replacement of the bionic healing abutment, it is usually necessary to remove the entire abutment from the implant before replacing it with a new one. Removing the entire healing abutment can easily damage the walls of the gingival cavity and disturb the initial implant-bone interface, disrupting the osseointegration foundation. This is especially true when the patient experiences rejection or when frequent abutment replacements are necessary, further increasing the risk of damage to the implant and the gingival cavity, thus hindering the dental implant procedure. Summary of the Invention

[0004] The purpose of this invention is to provide a biomimetic healing composite abutment for tissue engineering scaffold structures to address the aforementioned shortcomings of the prior art.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a biomimetic healing composite abutment for a tissue engineering scaffold structure, comprising an abutment body and a threaded connection portion, a tapered gingival portion, and a shaping end portion arranged sequentially from bottom to top on the abutment body, wherein the tapered gingival portion and the shaping end portion are connected by a connecting mechanism. The tapered gingival portion is provided with a docking platform on the side near the shaping end, the surface of the docking platform is provided with threaded short posts, and the bottom of the shaping end is provided with an internal threaded groove. The end of the shaping end is provided with an upper groove, a cylindrical groove, a shaft groove and a lower groove in sequence along its central axis. A pressure-applying disc is slidably connected to the inner side of the cylindrical groove. An upper block is fixedly connected to the top of the pressure-applying disc. A slot is formed on the top of the upper block. A connecting shaft is fixedly connected to the bottom of the pressure-applying disc. A lower block is fixedly connected to the bottom end of the connecting shaft. A docking groove corresponding to the lower block is formed on the surface of the docking platform. A support spring is installed between the pressure-applying disc and the inner wall of the cylindrical groove.

[0006] Furthermore, the specifications of the internal thread groove are adapted to the threaded short post.

[0007] Furthermore, the upper block is slidably connected to the inner side of the upper groove, and the shape and specifications of the upper block are adapted to the upper groove. The lower block is slidably connected to the inner side of the lower groove, and the shape and specifications of the lower block are adapted to the lower groove and the mating groove. A reserved gap is provided between the bottom end of the lower block and the opening of the lower groove.

[0008] Furthermore, the connecting shaft is a round shaft, and the connecting shaft is slidably connected to the inner side of the shaft groove.

[0009] Furthermore, one end of the support spring is fixedly connected to the bottom end of the inner wall of the cylindrical groove, and the other end of the support spring is fixedly connected to a support disk. The top of the support disk is provided with a number of balls in an annular shape at equal intervals, and the balls are in rolling connection with the lower surface of the pressure disk.

[0010] Furthermore, a biomimetic support layer is provided at the end of the shaping end. The biomimetic support layer includes a support portion disposed on the end face of the shaping end and a guide portion disposed on the periphery of the shaping end. A surface functional layer is provided on the outside of the biomimetic support layer.

[0011] Furthermore, the biomimetic scaffold layer uses a PLGA / PCL blend as a matrix, loads 10-20wt% hydroxyapatite nanoparticles, and the biomimetic scaffold layer has a biomimetic Haver tube structure with interconnected pores, a porosity of 60-80%, a pore size of 100-500μm, and a pore connectivity of ≥90%.

[0012] Furthermore, the thickness of the support portion is 2 mm, and the thickness of the guide portion is 100-500 μm.

[0013] Furthermore, the surface functional layer is composed of a cationic short peptide coating and a self-mineralization induction system. The amino acid sequence of the cationic short peptide is H(K)nS-RGD, where n=1-3. The coating thickness is 10-50μm. The self-mineralization induction system contains ovalbumin, tannic acid, and a calcium-phosphorus ion complex framework.

[0014] Furthermore, the surface functional layer is also loaded with 2-5 wt% antimicrobial peptide LL-37, which binds to the H(K)nS-RGD peptide segment through electrostatic interaction.

[0015] Compared with the prior art, the biomimetic healing composite abutment of the tissue engineering scaffold structure provided by the present invention has the following beneficial effects: 1. The biomimetic healing composite abutment of this tissue engineering scaffold structure is detachably set between the shaped end and the conical transgingival portion through a connecting mechanism. This allows the shaped end to be removed and replaced separately when redness and swelling occur at the gingival cuff, without the need to replace the entire healing abutment. This reduces the likelihood of affecting the stability of the implant and avoids damage to the initial implant-bone interface, thus facilitating the patient's dental implantation process.

[0016] 2. The biomimetic healing composite abutment of this tissue engineering scaffold structure, through the setting of the connecting mechanism, can switch its working mode as needed during use. This allows it to not only disassemble and assemble the abutment body as needed, but also to disassemble and assemble the shaped end separately as needed, thus making the use of the biomimetic healing abutment more flexible.

[0017] 3. The biomimetic healing composite abutment of this tissue engineering scaffold structure, through the setting of biomimetic scaffold layer and surface functional layer, not only improves the bone integration efficiency and has good biocompatibility during use, but also significantly enhances the antibacterial effect, thereby effectively promoting the growth and healing of gingival tissue. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this invention. For those skilled in the art, other drawings can be obtained based on these drawings.

[0019] Figure 1 This is a schematic diagram of the overall structure provided in an embodiment of the present invention; Figure 2 This is a schematic diagram of the structure of the tapered gingival portion and the molded end separated according to an embodiment of the present invention; Figure 3 This is a schematic diagram of the bottom view of the shaped end structure provided in an embodiment of the present invention; Figure 4 This is a schematic diagram of the longitudinal cross-sectional structure of the shaped end provided in an embodiment of the present invention; Figure 5 This is a schematic diagram of the internal structure of the shaped end provided in an embodiment of the present invention; Figure 6 A schematic diagram of the assembly structure of the upper block and the lower block provided in an embodiment of the present invention; Figure 7 This is a schematic diagram of the biomimetic scaffold layer and surface functional layer structure provided in an embodiment of the present invention; Figure 8 Provided for embodiments of the present invention Figure 7 Enlarged structural diagram at point A in the middle.

[0020] Explanation of reference numerals in the attached figures: 1. Base body; 101. Threaded connection part; 102. Conical through-gum part; 103. Molded end; 2. Docking platform; 21. Upper groove; 22. Cylindrical groove; 23. Shaft groove; 24. Lower groove; 25. Pressure disc; 26. Upper block; 27. Connecting shaft; 28. Lower block; 29. ​​Docking groove; 210. Threaded short post; 211. Internal threaded groove; 212. Support spring; 213. Slotted groove; 3. Reserved gap; 4. Support disc; 41. Ball bearing; 5. Bionic support layer; 501. Support part; 502. Guide part; 51. Surface functional layer. Detailed Implementation

[0021] To enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below with reference to the accompanying drawings.

[0022] Example 1: Please see Figure 1 - Figure 8 A biomimetic healing composite abutment for a tissue engineering scaffold structure includes an abutment body 1 and a threaded connection part 101, a tapered gingival portion 102 and a shaping end 103 arranged sequentially from bottom to top on the abutment body 1. The tapered gingival portion 102 and the shaping end 103 are connected by a connecting mechanism. A docking platform 2 is provided at the end of the tapered gingival portion 102 near the shaping end 103. A threaded short post 210 is provided on the surface of the docking platform 2, and an internal threaded groove 211 is provided at the bottom of the shaping end 103. The end of the molding end 103 is provided with an upper groove 21, a cylindrical groove 22, a shaft groove 23 and a lower groove 24 in sequence along its central axis. The diameter of the cylindrical groove 22 is greater than the length of the diagonal of the upper groove 21 and the lower groove 24. A pressure disc 25 is slidably connected to the inner side of the cylindrical groove 22. An upper block 26 is fixedly connected to the top of the pressure disc 25. A slot 213 is opened on the top of the upper block 26. A connecting shaft 27 is fixedly connected to the bottom of the pressure disc 25. A lower block 28 is fixedly connected to the bottom of the connecting shaft 27. A docking groove 29 corresponding to the lower block 28 is opened on the surface of the docking platform 2. A support spring 212 is installed between the pressure disc 25 and the inner wall of the cylindrical groove 22.

[0023] It should be noted that the specifications of the internal thread groove 211 are compatible with the threaded short post 210, so that with the cooperation of the threaded short post 210 and the internal thread groove 211, the shaped end 103 can be effectively assembled and connected with the tapered through-gum part 102.

[0024] Furthermore, the upper block 26 is slidably connected to the inner side of the upper groove 21, and the shape and specifications of the upper block 26 are adapted to the upper groove 21. The lower block 28 is slidably connected to the inner side of the lower groove 24, and the shape and specifications of the lower block 28 are adapted to the lower groove 24 and the mating groove 29. A reserved gap 3 is provided between the bottom end of the lower block 28 and the groove opening of the lower groove 24, so that when the upper block 26 is rotated by inserting a tool into the slot 213 to apply rotational force to rotate the shaping end 103, the reserved gap 3 allows for tolerance of the pressure stroke distance value of the lower block 28. Thus, while ensuring that a stable rotational force can be applied to the upper block 26, the lower block 28 will not enter the mating groove 29, resulting in better stability when the shaping end 103 is disassembled and assembled separately.

[0025] In addition, the connecting shaft 27 is a round shaft, and the connecting shaft 27 is slidably connected to the inner side of the shaft groove 23.

[0026] It should be further explained that the pre-tightening force when installing the abutment body 1 and the implant is much greater than the pre-tightening force between the shaping end 103 and the conical transgingival portion 102, so that the disassembly and assembly of the shaping end 103 will not affect the connection stability between the abutment body 1 and the implant.

[0027] When it is necessary to disassemble the molded end 103 separately, the operator inserts a tool into the slot 213, then applies pressure to it gently and rotates it so that the lower block 28 is always in the lower slot 24. At this time, under the transmission action of the upper slot 21, the upper block 26 can drive the molded end 103 to rotate synchronously when it rotates, so that the threaded short post 210 and the internal threaded groove 211 gradually separate, thereby allowing the molded end 103 to be removed.

[0028] When it is necessary to disassemble the abutment body 1, the operator inserts a tool into the slot 213 and applies pressure to it, causing the upper block 26 to move from the upper slot 21 to the cylindrical slot 22, so that the rotation of the upper block 26 no longer applies rotational force to the shaping end 103. When the upper block 26 moves, it drives the connecting shaft 27 to move, so that the lower block 28 can move synchronously with the connecting shaft 27 until it is fully inserted into the docking slot 29. At this time, the operator applies rotational force to the upper block 26, causing the lower block 28 to rotate synchronously and apply rotational force to the conical perforating part 102. Thus, the abutment body 1 can be separated from the implant without affecting the stability between the shaping end 103 and the conical perforating part 102.

[0029] Example 2: Please see Figure 1 - Figure 3This embodiment provides a technical solution based on the above embodiments: one end of the support spring 212 is fixedly connected to the bottom end of the inner wall of the cylindrical groove 22, and the other end of the support spring 212 is fixedly connected to the support disk 4. The top of the support disk 4 is provided with a number of balls 41 arranged in a circular shape at equal intervals. The balls 41 are in rolling connection with the lower surface of the pressure disk 25, so that when the base body 1 is disassembled and assembled, the rotation of the upper block 26 will not have a large frictional resistance with the shaping end 103, thereby not affecting the stability of the shaping end 103.

[0030] Example 3: This embodiment provides a technical solution based on the above embodiment: a bionic support layer 5 is provided at the end of the shaping end 103, the bionic support layer 5 includes a support portion 501 provided on the end face of the shaping end 103 and a guide portion 502 provided on the periphery of the shaping end 103, and a surface functional layer 51 is provided on the outside of the bionic support layer 5.

[0031] It should be noted that the biomimetic scaffold layer 5 uses PLGA / PCL blend as matrix, loads 10-20wt% hydroxyapatite nanoparticles, and is prepared by DLP photopolymerization 3D printing. It has a biomimetic Haver tube structure with interconnected pores, a porosity of 60-80%, a pore size of 100-500μm, and a pore connectivity of ≥90%. In addition, the surface functional layer 51 is attached to the surface of the biomimetic scaffold layer 5 and also penetrates into its pores.

[0032] Furthermore, the PLGA / PCL blend mass ratio of the biomimetic scaffold layer 5 is 3:2-2:3, the particle size of the hydroxyapatite nanoparticles is 50-200nm, and the surface is grafted with methacrylate groups to achieve covalent cross-linking with the matrix.

[0033] It should be further explained that the thickness of the support part 501 is 2 mm, which enables the support part 501 to provide stable support for the entire bionic scaffold layer 5. The thickness of the guide part 502 is 100-500 μm, so that after the guide part 502 is attached, there will be no obvious thickness difference on the surface of the shaped end 103.

[0034] In this embodiment, the surface functional layer 51 is composed of a cationic short peptide coating and a self-mineralization induction system. The amino acid sequence of the cationic short peptide is H(K)nS-RGD, where n=1-3. The coating thickness is 10-50μm. The self-mineralization induction system contains ovalbumin, tannic acid, and a calcium-phosphorus ion complex framework. Through the ovalbumin-tannic acid-calcium-phosphorus ion framework, the abutment achieves continuous mineralization in vivo, and the modulus gradually increases from 10-30kPa to 42kPa, matching the dynamic mechanical requirements of the extracellular matrix during osteogenic processes.

[0035] It should be added that the surface functional layer 51 is also loaded with 2-5 wt% of the antimicrobial peptide LL-37, which binds to the H(K)nS-RGD peptide through electrostatic interaction, thereby significantly enhancing the antimicrobial activity of the surface functional layer 51 against Staphylococcus aureus and Escherichia coli. Furthermore, the antimicrobial peptide LL-37 loaded on the surface functional layer 51 works synergistically with the RGD peptide to both inhibit bacterial adhesion and promote osteoblast proliferation and differentiation.

[0036] It should be noted that the preparation method of surface functional layer 51 is a two-step immersion method: the first step is to immerse the abutment in a PBS solution containing 5-10 mg / mL H(K)nS-RGD peptide and adsorb at 37℃ for 2-4 h; the second step is to immerse it in an ovalbumin-calcium ion composite solution (ovalbumin concentration 20-50 mg / mL, calcium ion concentration 10-20 mmol / L) for 1 h, and then transfer it to a tannic acid-phosphate composite solution (tannic acid concentration 5-15 mg / mL, phosphate concentration 10-20 mmol / L) for 1 h to form an organic-inorganic composite coating.

[0037] The aforementioned biocompatible adhesive is a GelMA prepolymer with a substitution rate of 60-90%, with 0.5wt% photoinitiator LAP added. It achieves interlayer bonding through blue light curing and has a shear strength ≥15MPa.

[0038] The foregoing has only described certain exemplary embodiments of the present invention by way of illustration. Undoubtedly, those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the foregoing drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A biomimetic healing composite abutment for a tissue engineering scaffold structure, comprising an abutment body (1) and, from bottom to top, a threaded connection portion (101), a tapered transgingival portion (102), and a molded end portion (103) sequentially disposed on the abutment body (1), characterized in that, The tapered gingival portion (102) and the shaped end portion (103) are connected by a connecting mechanism; The tapered gingival portion (102) is provided with a docking platform (2) on the side near the shaping end (103). The surface of the docking platform (2) is provided with a threaded short post (210), and the bottom of the shaping end (103) is provided with an internal threaded groove (211). The end of the shaping end (103) is provided with an upper groove (21), a cylindrical groove (22), a shaft groove (23) and a lower groove (24) in sequence along its central axis. A pressure disc (25) is slidably connected to the inner side of the cylindrical groove (22). An upper block (26) is fixedly connected to the top of the pressure disc (25). A slot (213) is opened on the top of the upper block (26). A connecting shaft (27) is fixedly connected to the bottom of the pressure disc (25). A lower block (28) is fixedly connected to the bottom of the connecting shaft (27). A docking groove (29) corresponding to the lower block (28) is opened on the surface of the docking platform (2). A support spring (212) is installed between the pressure disc (25) and the inner wall of the cylindrical groove (22).

2. The biomimetic healing composite abutment of a tissue engineering scaffold structure according to claim 1, characterized in that, The specifications of the internal thread groove (211) are compatible with the threaded short post (210).

3. The biomimetic healing composite abutment of a tissue engineering scaffold structure according to claim 2, characterized in that, The upper block (26) is slidably connected to the inner side of the upper groove (21), and the shape and specifications of the upper block (26) are adapted to the upper groove (21). The lower block (28) is slidably connected to the inner side of the lower groove (24), and the shape and specifications of the lower block (28) are adapted to the lower groove (24) and the mating groove (29). A reserved gap (3) is provided between the bottom end of the lower block (28) and the opening of the lower groove (24).

4. The biomimetic healing composite abutment of a tissue engineering scaffold structure according to claim 3, characterized in that, The connecting shaft (27) is a round shaft, and the connecting shaft (27) is slidably connected to the inner side of the shaft groove (23).

5. The biomimetic healing composite abutment of a tissue engineering scaffold structure according to claim 4, characterized in that, One end of the support spring (212) is fixedly connected to the bottom end of the inner wall of the cylindrical groove (22), and the other end of the support spring (212) is fixedly connected to the support disc (4). The top of the support disc (4) is provided with a number of balls (41) in a circular shape at equal intervals. The balls (41) are in rolling connection with the lower surface of the pressure disc (25).

6. The biomimetic healing composite abutment of a tissue engineering scaffold structure according to claim 1, characterized in that, The end of the shaping end (103) is provided with a bionic support layer (5). The bionic support layer (5) includes a support portion (501) provided on the end face of the shaping end (103) and a guide portion (502) provided on the periphery of the shaping end (103). The outside of the bionic support layer (5) is provided with a surface functional layer (51).

7. The biomimetic healing composite abutment of a tissue engineering scaffold structure according to claim 6, characterized in that, The biomimetic scaffold layer (5) uses PLGA / PCL blend as matrix and loads 10-20wt% hydroxyapatite nanoparticles. The biomimetic scaffold layer (5) has biomimetic Haver tube structure with interconnected pores, porosity of 60-80%, pore size of 100-500μm, and pore connectivity ≥90%.

8. The biomimetic healing composite abutment of a tissue engineering scaffold structure according to claim 7, characterized in that, The thickness of the support part (501) is 2 mm, and the thickness of the guide part (502) is 100-500 μm.

9. The biomimetic healing composite abutment of a tissue engineering scaffold structure according to claim 8, characterized in that, The surface functional layer (51) is composed of a cationic short peptide coating and a self-mineralization induction system. The amino acid sequence of the cationic short peptide is H(K)nS-RGD, where n=1-3. The coating thickness is 10-50μm. The self-mineralization induction system contains ovalbumin, tannic acid, and a calcium-phosphorus ion complex framework.

10. The biomimetic healing composite abutment of a tissue engineering scaffold structure according to claim 9, characterized in that, The surface functional layer (51) is also loaded with 2-5 wt% antimicrobial peptide LL-37, which binds to the H(K)nS-RGD peptide segment through electrostatic interaction.

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