Insecticidal composition and application thereof

Abstract

The invention belongs to the technical field of agricultural insecticide, and discloses an insecticidal composition and application thereof.The active ingredient of the insecticidal composition comprises an active ingredient A and an active ingredient B. The active ingredient A is a compound shown in the formula I, the active ingredient B is any one of nicofluprole, dimpropyridaz, flometoquin and inazopyraroxamet, and the mass ratio of the active ingredient A to the active ingredient B is 1: 55-60: 1. The insecticidal composition disclosed by the invention has the advantages of synergism, resistance reduction and the like on various pests, and can be used for preventing and treating agricultural, forestry and gardening pests.

Description

Technical Field

[0001] This invention belongs to the field of pesticide insecticide technology and discloses an insecticidal composition and its application. Background Technology

[0002] Compound I is a novel compound independently developed by our company. This compound has a broad insecticidal spectrum, low toxicity, and high efficacy. The structure of compound I is shown below:

[0003]

[0004] In agricultural production, chemical control remains the primary method for pest control. However, pesticide resistance has become a major challenge. To address this, rationally combining existing pesticides can effectively protect these pesticides and extend their lifespan. Therefore, the inventors explored the synergistic effects of compound I with any one of nicofluprole, dimpropyridaz, flocetoquin, or indazapyroxamet on various pests. This research aims to provide a theoretical basis for reducing pesticide usage and improving control efficacy. However, there are currently no reports on the control effects of compound I combined with nicofluprole, dimpropyridaz, flocetoquin, or indazapyroxamet. Summary of the Invention

[0005] To address the aforementioned problems in the prior art, the present invention provides an insecticidal composition that combines compounds with different mechanisms of action in a suitable mass ratio. This composition exhibits a significant synergistic effect on various agricultural pests, resulting in remarkable control efficacy, effectively reducing pesticide usage, and slowing the development of pesticide resistance in pests.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: an insecticidal composition and its application, wherein the active ingredients of the insecticidal composition comprise active ingredient A and active ingredient B, and active ingredient A is a compound of formula I: The active ingredient B is any one of nicofluprole, dimpropyridaz, frometoquin, and indazapyroxamet, and the mass ratio of active ingredient A to active ingredient B is 1:55 to 60:1, or any value between the above values.

[0007] Furthermore, the active ingredient B is nicofluprole, and the mass ratio of the compound of formula I to nicofluprole is 1:40 to 42:1, or any value between the above values;

[0008] The active ingredient B is dimpropyridaz, and the mass ratio of the compound of formula I to dimpropyridaz is 1:48 to 48:1, or any value between the above values.

[0009] The active ingredient B is floctoquin, and the mass ratio of the compound of formula I to floctoquin is 1:48 to 32:1, or any value between the above values;

[0010] The active ingredient B is indazapyroxamet, and the mass ratio of the compound of formula I to indazapyroxamet is 1:50 to 20:1, or any value between these values.

[0011] Furthermore, the active ingredient B is nicofluprole, and the mass ratio of the compound of formula I to nicofluprole is 1:28 to 25:1, or any value between the above values;

[0012] The active ingredient B is dimpropyridaz, and the mass ratio of the compound of formula I to dimpropyridaz is 1:36 to 36:1, or any value between the above values.

[0013] The active ingredient B is floctoquin, and the mass ratio of the compound of formula I to floctoquin is 1:32 to 32:1, or any value between the above values;

[0014] The active ingredient B is indazapyroxamet, and the mass ratio of the compound of formula I to indazapyroxamet is 1:40 to 15:1, or any value between the above values;

[0015] Furthermore, the active ingredient B is nicofluprole, and the mass ratio of the compound of formula I to nicofluprole is 1:16 to 15:1, or any value between the above values;

[0016] The active ingredient B is dimpropyridaz, and the mass ratio of the compound of formula I to dimpropyridaz is 1:20 to 35:1, or any value between the above values;

[0017] The active ingredient B is floctoquin, and the mass ratio of the compound of formula I to floctoquin is 1:30 to 30:1, or any value between the above values;

[0018] The active ingredient B is indazapyroxamet, and the mass ratio of the compound of formula I to indazapyroxamet is 1:15 to 15:1, or any value between these values.

[0019] Furthermore, the total weight of the insecticidal composition is 100 wt%, and the total weight of active ingredient A and active ingredient B accounts for 0.5 wt% to 80 wt% of the total weight of the insecticidal composition.

[0020] Furthermore, the insecticidal composition is prepared into an agriculturally permissible formulation, wherein the formulation is a solid formulation or a liquid formulation;

[0021] Furthermore, the solid dosage forms include powders, granules, balls, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets, or soluble granules;

[0022] The liquid formulations include soluble agents, colloids, oils, spreading oils, emulsions, latexes, dispersible liquids, ointments, water emulsions, oil emulsions, microemulsions, lipid suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspensions, microcapsule suspension-suspension agents, microcapsule suspension-water emulsions, or microcapsule suspension-suspension emulsions.

[0023] Furthermore, the solid formulation is a wettable powder or a water-dispersible granule, and the liquid formulation is any one of a suspension, emulsifiable concentrate, dispersible oil suspension, water emulsion, or microemulsion.

[0024] Furthermore, in addition to the active ingredient, the insecticidal composition also includes auxiliary ingredients, which are selected from one or more of the following: wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, preservatives, stabilizers, synergists, or carriers.

[0025] The wetting agent is selected from one or more of the following: alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium dodecyl sulfate, sodium dioctyl succinate sulfonate, α-olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol ethoxylate, sodium fatty alcohol polyoxyethylene ether sulfate, silkworm excrement, soapberry powder, soapberry powder, SOPA, detergent, emulsifier 2000 series, and wetting and penetrating agent F; and / or

[0026] The dispersant is selected from one or more of the following: lignin sulfonates, alkyl naphthalene sulfonates formaldehyde condensates, naphthalene sulfonates, tristyrylphenol ethoxylate phosphates, fatty alcohol ethoxylates, alkylphenol polyoxyethylene ethers, alkylphenol polyoxyethylene ether methyl ether condensates sulfates, fatty amine polyoxyethylene ethers, glycerol fatty acid ester polyoxyethylene ethers, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers, and EO-PO graft copolymers; and / or

[0027] The emulsifier is selected from one or more of the following: calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrene-phenol polyoxyethylene ether, castor oil polyoxyethylene ether, and alkylphenol ether phosphate; and / or

[0028] The thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose, and silica; and / or

[0029] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid, and tartaric acid; and / or

[0030] Antifreeze is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons, and inorganic salts; and / or

[0031] Defoamer selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of the fatty alcohols; and / or

[0032] The solvent is selected from one or more of benzene, toluene, xylene, mesitylene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, hydrocarbon carbonates, diesel oil, solvent oil, vegetable oil, vegetable oil derivatives, and water; and / or

[0033] The preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, Kathon, and 1,2-benzisothiazolin-3-one; and / or

[0034] The stabilizer is selected from one or more of the following: disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, silica, talc, montmorillonite, and starch; and / or

[0035] Synergists are selected from synergistic phosphorus, synergistic ether; and / or

[0036] The carrier is selected from one or more of the following: ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils, and vegetable oil derivatives.

[0037] The present invention also discloses the application of the insecticidal composition described above for the control of plant pests.

[0038] Furthermore, the plant pests mentioned are lepidopteran pests and hemiptera pests.

[0039] Furthermore, the Lepidoptera mentioned include, but are not limited to, the diamondback moth (Plutellaxylostella), the small diamondback moth (Plutellaxylostella), the grape leafroller (Polychrosis viteana), the citrus leafroller (Prays endocarpa), the olive leafroller (Prays oleae), species of Pseudaletia (Pseudaletia spp.) (noctus moth), the armyworm (Pseudaletia unipunctata), the soybean looper (Pseudoplusia includes), the inchworm (Rachiplusia nu), the rice stem borer (Scirpophaga incertulas), species of Sesamia (Sesamia spp.) (stem borers), the pink rice stem borer (Sesamia inferens), and the rice stem borer (Sesamia inferens). The following species are listed: *Spodoptera nonagrioides*, *Setora nitens*, *Sitotroga cerealella* (Angoumois grain moth), *Sparganothis pilleriana*, *Spodoptera spp.* (noctuid moth), *Spodoptera exigua* (beetarmyworm), *Spodoptera fugiperda* (fall armyworm), *Adoxophyes spp.*, *Adoxophyes orana*, and *Agrotis spp.*(Root cutter), small groundworm (Agrotis ipsilon) (black cutworm), cotton leaf looper (Alabama argillacea), Amorbia cuneana, Amyelosis transitella (navel orange worm), peach twig borer (Anarsia lineatella), yellow hemp looper (Anomissabulifera) (jute looper), bean looper (Anticarcia gemma ta lis) (velvet beancate r pillar), fruit tree leaf roller (Archi ps argyrospila) (fruittree leafroller), rose leaf roller (Archips rosana) (rose leaf roller), tortricidmoths (Argyrotaenia spp.) (tortricidmoths), citrus leaf roller (Argyrotaenia citrana), Autographa gamma, Bonagota cranaodes, Borbo butterflies The following species are listed: *Cinnamonara*, *Caloptilia* spp. (leaf miners), *Capuareticulana*, *Carposina niponensis* (peach fruit moth), *Chilospp.*, *Chlumetia transversa* (mango shoot borer), *Choristoneura rosaceana* (obliquebanded leafroller), *Chrysodeixis* spp., *Cnaphalocerus medinalis* (grass leafroller), *Colias* spp., *Conpomorpha cramerella* (lychee leaf moth), *Cossus cossus*, and *Crambus* spp.(Sod webworms), Plum fruit moth (Cydiafunebrana), Oriental fruit moth (Cydia molesta), Pea moth (Cydia nignicana), Apple leafroller (Cydiapomonella), Darna diducta, Stem borers (Diaphania spp.), Stalk borers (Diatraea spp.), Sugarcane borer (Diatraea saccharalis), Southwest corn stalk borer (Diatraea graniosella), Cotton bollworm (Earias spp.), Egyptian corn bollworm (Earias) *Egyptian bollworm* (*E. insulata*), *Rough northern bollworm* (*Earias vitella*), *Ecdytopophaaurantianum*, *Lesser cornstalk borer* (*Elasmopalpus lignosellus*), *Flour moths* (*Ephestia spp.*), *Almond moth* (*Ephestia cautella*), *Tobacco moth* (*Ephestia elutella*), *Mediterranean flour moth* (*Ephestia kuehniella*), *Epimeces spp.*, *Epinotia aporema*, *Banana skipper* (*Erionota thrax*), *Eupoecilia ambiguella*, *Euxoa* *Auxiliaris* (army cutworm), species of the genus *Feltia*, and species of the genus *Gortyna*.(Stemborers), Oriental fruit moth (Grapholita molesta), Hedylepta indicate, bean leaf webber, Helicoverpas p p. (noctus), cotton bollworm (Helicover paarmigera), cereal noctus (Helicoverpa zea), Heliothis spp. (noctus), tobacco bud noctus (Heliothis virescens), tobacco budworm (Hellula undalis), cabbage webworm (Hellula undalis), Indarbela spp., root borers, tomato codling moth (Keiferia lycopersicella), tomato pinworm, Leucinodes orbonalis (eggplant fruit) * *Borr*, *Leucoptera malifoliella*, *Lithocollectis* spp., *Lobesia botrana* (grape fruit moth), *Loxagrotis* spp. (noctus moth), *Loxagrotis albicosta* (western bean cutworm), *Lymantria dispar* (gypsy moth), *Lyonetia clerkella* (apple leaf miner), *Mahasena corbetti* (oil palm bagworm), and *Malacosoma* spp.(tent caterpillars), cabbage armyworm (Mamestra brassicae), bean pod borer (Maruca testulalis), bagworm (Metisa plana), true armyworm (Mythimna unipuncta), small tomato borer (Neoleucinodes elegantalis), winter moth (Operophthera brumata), European corn borer (Ostrinia nubilalis), Pandemis cerasana, common curranttortrix, brown apple tortrix (Pandemis heparana), African swallowtail butterfly (Papilio demodocus), pink bollworm (Pectinophora gossypiella), species of Peridroma (Peridroma) spp. (root cutter), Peridroma saucia (variegated cutworm), Perileucoptera coffeella (white coffee leafminer), Phthorimaea operculella (potatotuber moth), Phyllocnisitis citrella (citrus leafminer), Phyllonorycter spp. (leaf miner), Pieris rapae (imported cabbage worm), Plathypena scabra, Plodia interpunctella (Indian meal moth), Spodoptera oridania (southern armyworm), Synanthedon spp.(root borers), Thecla basilides, Thermisiagemmatalis, clothes moth (Tineola bisselliella) (webbing clothes moth), pink leafminer (Trichoplusia ni) (cabbage worm), tomato leafminer (Tuta absoluta), nest moth (Yponomeutaspp.), coffee leopard borer (Zeuzera coffeae) (red branch borer), and pear leopard borer (Zeuzera pyrina) (leopard moth).

[0040] The aforementioned Hemiptera pests include, but are not limited to, the following stink bugs: *Acrosternumhilare* (green stink bug), *Blissus leucopterus* (chinch bug), *Calocoris norvegicus* (potato mirid bug), *Cimex hemipterus* (tropical bed bug), *Cimexlectularius* (bed hug bug), *Daghertus fasciatus*, *Dichelops furcatus*, *Dysdercus suturellus* (cotton stainer bug), *Edessa meditabunda*, *Eurygaster maura* (cereal bug), and *Euschistus*. heroes, brown stink bug (Euschistusservus), Helopeltis antonii, tea blight plant bug (Helopeltis theivora), Lagynotomus spp. (stink bug), Leptocorisa oratorius, Leptocorisa varicornis, Lygusspp. (plant bug), Lygus hesperus (western tarnished plant bug), Maconellicoccus hirsutus, Neurocolpus longirostris, southern green stink bug (Nezara viridula), PhyLocoris spp.(Mirid bug), California plant mirid bug (Phytocoris californicus), Phytocoris relativus, Piezodorus guildingi, four-lined plant bug (Poecilocapsus lineatus), Psallus vaccinicola, Pseudacysta perseae, Scaptocoris castanea and Triatoma spp. (bloodsucking conenose bug / kissing bug), pea aphid (Acrythosiphonpisum), adolphid (Adelges spp.), cabbage whitefly (Aleurodes proletella), spiral whitefly (Aleurodicus disperses), woolly whitefly (Aleurothrixus flccosus) Whitefly), Aluacaspis spp., Amrasca bigutella bigutella, Aphrophora spp. (leafhopper), Aonidiella aurantii (California redscale), Aphis spp. (aphid), Aphis gossypii (cotton aphid), Aphis pomi (apple aphid), Aulacorthitm solani (foxgloveaphid), Bemisia spp. (whitefly), Bemisia argentifolii, Bemisia tabaci (sweetpotato whitefly), Brachycolusnoxius (Russian aphid) The species *Brachycorynclia asparagi* (asparagusaphid), *Brevennia rehi*, *Brevicoryne brassicae* (cabbage aphid), and *Ceroplastes spp.*(Scale insects), Red wax scale (Ceroplastes rubens), Snow shield scale (Chionaspis spp.), Round shield scale (Chrysomphalus spp.), Soft wax scale (Coccus spp.), Rosy apple aphid (Dysaphis plantaginea), Leafhopper (Empoasca spp.), Woolly apple aphid (Eriosomalanigerum), Cottony cushion scale (Icerya purchasi), Mango yellow-lined leafhopper (Idioscopus nitidulus), Smaller brown planthopper (Laodelphax striatellus), Oyster shield scale (Lepidosaphes spp.), Macrosiphum spp.), Macrosiphum euphorbiae (potato aphid), Macrosiphum granarium (English grain aphid), Macrosiphum rosae (rose aphid), Macrosteles quadrilineatus (asterleafhopper), Mahanarva frimbiolata, Metopolophium dirhodum (rose grain aphid), Midis longicornis, Myzuspersicae (greenpeach aphid), Nephotettix spp.(Leafhopper), Nephotettixcinctipes (green leafhopper), Nilaparvata lugens (brownplanthopper), Parlatoria pergandii (chaff scale), Parlatoria ziziphi (ebony scale), Peregrinus maidis (corndelphacid), Philaenus spp. (blower), Phylloxera vitifoliae (grape phylloxera), Phylsokermes piceae (spruce budscale), Planococcus spp. (meat mealybug), Pseudococcus spp. (meat mealybug), Pscudococcus brcvipcs (pinc apple) * *Mcalybug*, *Quadraspidiotus perniciosus* (San Jose scale), *Rhapalosiphum spp.* (aphid), *Rhapalosiphummaida* (corn leaf aphid), *Rhapalosiphumpadi* (oatbird-cherry aphid), *Saissetia spp.* (scale insect), *Saissetiaoleae* (black scale insect), *Schizaphis graminum* (grass aphid), *Sitobion avenge* (British wheat aphid), *Sogatella furcifera* (white-backed planthopper), *Therioaphis* spp. (aphids), Toumeyella spp. (scale insects), Toxoptera spp. (aphids), Trialeurodes spp. (whiteflies).(Whitefly), *Trialeurodesvaporariorum* (greenhouse whitefly), *Trialeurodesabutiloneus* (bandedwing whitefly), *Unaspis spp.* (scale insect), *Unaspisyanonensis* (arrowhead scale), and *Zulia entreriana*.

[0041] In particular, the insecticidal composition of the present invention has excellent control effects on diamondback moth, beet armyworm, rice stem borer, whitefly, rice planthopper, aphid, etc., and exhibits a synergistic effect within the above-mentioned mass ratio range.

[0042] Furthermore, the plant pests mentioned are diamondback moth and aphid.

[0043] The beneficial effects of this invention are as follows:

[0044] 1) This invention rationally combines compounds with different mechanisms of action, which have a significant synergistic effect on a variety of pests at a certain mass concentration, thus significantly improving the control effect;

[0045] 2) The insecticidal composition of the present invention significantly reduces the amount of pesticides used, reduces pesticide residues, and reduces environmental pollution. Detailed Implementation

[0046] To make the technical solution, objectives and advantages of the present invention clearer, the present invention is described with reference to the following specific embodiments. However, the present invention can be implemented in various forms and should not be limited to the embodiments described herein.

[0047] Formulation preparation example:

[0048] Formulation Preparation Example 1: 18% Formula I compound·dimpropyridaz suspension (1:5)

[0049] Formula composition: 3% Formula I compound, 15% dimpropyridaz, 4% Gelbert alcohol polyoxyethylene ether, 2% sodium lignosulfonate, 3% tristyrene-phenylphenol polyoxyethylene ether phosphate, 0.5% sodium p-hydroxybenzoate, 0.25% xanthan gum, 5% ethylene glycol, 1% magnesium aluminum silicate, 0.02% organosilicon, deionized water to make up the balance;

[0050] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, water is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenization filtration to obtain the suspension product.

[0051] Formulation Preparation Example 2: 27% Formula I compound·dimpropyridaz water-dispersible granules (8:1)

[0052] Formula composition: 24% Formula I compound, 3% dimpropyridaz, 5% sodium alkyl polyoxyethylene ether sulfonate, 10% sodium lignosulfonate, 4% sodium dodecyl sulfate, 10% ammonium sulfate, starch to make up the balance;

[0053] Preparation method: According to the formula ratio, add the active ingredients to the carrier, and add surfactants and other functional additives to it. Mix, and after air jet pulverization, add 10-25% water. Then knead, granulate, dry and sieve to obtain water-dispersible granules; or spray water, granulate and dry the pulverized powder in a fluidized bed granulator, and then sieve to obtain the product.

[0054] Formulation Preparation Example 3: 24% Formula I compound nicofluprole suspension (1:2)

[0055] Formula composition: 8% Formula I compound, 16% nicofluprole, 3% sodium dodecyl sulfate, 2% styrene-phenol polyoxyethylene ether sulfate, 2% fatty alcohol polyoxyethylene ether phosphate, 0.25% xanthan gum, 1% magnesium aluminum silicate, 5% propylene glycol, 0.1% sodium sorbate, 0.5% silicone oil, deionized water to make up the balance;

[0056] Preparation method: Same as in preparation example 1.

[0057] Formulation Preparation Example 4: 32% Formula I compound nicofluprole wettable powder (7:1)

[0058] Formula composition: 28% Formula I compound, 4% nicofluprole, 3% sodium alkylnaphthalene sulfonate, 4% succinate sulfonate, 3% sodium dodecyl sulfate, 5% silica, 20% starch, kaolin to make up the balance;

[0059] Preparation method: According to the formulation ratio in the example, the active ingredients are added to the carrier, and surfactants and other functional additives are added thereto. After mixing, the mixture is pulverized by air jet and then mixed again to obtain a wettable powder product.

[0060] Formulation Preparation Example 5: 22.5% Formula I compound·indazapyroxamet suspension (7:2)

[0061] Formulation composition: 17.5% Formula I compound, 5% indazapyroxamet, 2% styrene-phenol polyoxyethylene ether, 3% dehydrated sorbitan oleate polyoxyethylene ether, 3% styrene-phenol polyoxyethylene ether sulfate, 1% sodium polycarboxylate, 1.5% magnesium aluminum silicate, 0.1% carboxyethyl cellulose, 1% sodium sorbate, 5% glycerol, 0.5% silicone oil, deionized water to make up the balance;

[0062] Preparation method: Same as in preparation example 1.

[0063] Formulation Preparation Example 6: 1.5% Formula I compound·indazapyroxamet water emulsion (1:5)

[0064] Formulation composition: 0.25% Formula I compound, 1.25% indazapyroxamet, 10% thiol, 12% cyclohexanone, 0.5% EO / PO block copolymer, 1% tristyrene-phenol polyoxyethylene ether phosphate, 5% ethylene glycol, 1% glycerol, 0.05% silicone, 0.2% xanthan gum, 0.1% sodium benzoate, deionized water to make up the balance;

[0065] Preparation method: According to the formula ratio, the active ingredients are dissolved in the solvent and emulsifier is added to form a homogeneous oil phase. Deionized water and antifreeze are mixed together to form a homogeneous aqueous phase. Under high-speed shearing, the aqueous phase is added to the oil phase to form a well-dispersed water emulsion product.

[0066] Formulation Preparation Example 7: 20.4% Flometoquin (Formula I) suspension (2:15)

[0067] Formula composition: 2.4% Formula I compound, 18% flometoquin, 1% sodium dodecyl sulfate, 1% fatty alcohol polyoxyethylene ether sulfate, 3% alkylphenol polyoxyethylene ether phosphate, 0.2% xanthan gum, 1% magnesium aluminum silicate, 4% propylene glycol, 0.01% potassium benzisothiazolinone, 0.5% silicone oil, deionized water to make up the balance;

[0068] Preparation method: Same as in preparation example 1.

[0069] Formulation Preparation Example 8: 11% Flometoquin Formula I Dispersible Oil Suspension (10:1)

[0070] Formula composition: 10% Formula I compound, 1% flometoquin, 5% tristyrene-phenylphenol polyoxyethylene ether polyoxypropylene ether, 9% castor oil polyoxyethylene ether, 3% calcium dodecylbenzene sulfonate, 2% succinate sulfonate, 1% magnesium aluminum silicate, 1% Tesco 869, corn oil to make up the balance;

[0071] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, vegetable oil is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenization filtration to obtain the dispersible oil suspension product.

[0072] Example 1: Indoor bioactivity assay of diamondback moth

[0073] The experiment was conducted in accordance with NY / T1154.14-2008 "Guidelines for Indoor Bioassay of Pesticides - Insecticides Part 14: Leaf Dipping Method" and NY / T1154.7-2006 "Guidelines for Indoor Bioassay of Pesticides - Insecticides Part 7: Determination of Combined Effects of Mixtures".

[0074] Experimental subject: Diamondback moth (Plutella xylostella), third instar larvae.

[0075] Test reagents: Formula I compound, nicofluprole, dimpropyridaz, frometoquin, and indazapyroxamet technical grade.

[0076] Drug preparation: First, dissolve the active pharmaceutical ingredient in a suitable solvent to prepare a high-concentration stock solution. Then, according to the drug activity, prepare a mixed solution by mixing each individual agent in an appropriate proportion. Dilute the above individual agents and mixed solutions with an aqueous solution containing 0.1% Tween 80 to obtain five series of mass concentrations.

[0077] Experimental Methods: Cabbage leaves that had not been treated with any pesticides were collected, rinsed thoroughly with clean water, and air-dried. The cabbage leaves were then cut into suitable leaf discs, placed in the test solution for 10 seconds, removed, and allowed to air-dry naturally before being placed in petri dishes. Twenty healthy, uniformly sized diamondback moth larvae were inoculated into each dish. Each concentration treatment was replicated four times, with a pesticide-free treatment serving as a blank control. After treatment, the insects were reared in an artificial climate chamber at a temperature of (25±1)℃ and a photoperiod of 16h:8h (light:dark).

[0078] Data survey: The mortality of test insects was investigated 48 hours after pesticide treatment. The criteria for judging insect mortality were obvious shrinkage of the insect body or inability to crawl normally when pricked. The total number of insects and the number of dead insects were recorded.

[0079] Calculation method:

[0080] Calculate the mortality rate for each treatment based on the survey data. Use the following formula:

[0081]

[0082] In the formula:

[0083] P – Mortality rate, expressed as a percentage (%);

[0084] K represents the number of dead insects, in heads;

[0085] N represents the total number of insects treated, in units of heads.

[0086]

[0087] In the formula:

[0088] P1—Adjusted mortality rate, in percentage (%);

[0089] P t —The mortality rate is expressed as a percentage (%).

[0090] P0 – Mortality rate in the blank control group, expressed as a percentage (%).

[0091] If the control mortality rate is <5%, no correction is needed; if the control mortality rate is between 5% and 20%, correction should be performed according to the corrected mortality rate formula; if the control mortality rate is >20%, the trial needs to be repeated.

[0092] The toxicity regression equation and LC were obtained using a statistical analysis system. 50 The values ​​and correlation coefficients are used to evaluate the activity of the test reagent on the biological sample.

[0093] The co-toxicity coefficient (CTC value) of the mixture is calculated using the following formula:

[0094]

[0095] In the formula:

[0096] ATI – Actual Measured Toxicity Index of Mixtures;

[0097] S – LC50 of standard insecticides 50 The unit is milligrams per liter (mg / L);

[0098] M – LC of the mixture 50 The unit is milligrams per liter (mg / L).

[0099] TTI = TI A ×P A +TI B ×P B

[0100] In the formula:

[0101] TTI – Theoretical Toxicity Index of Mixtures;

[0102] TI A —A. Toxicity index of drug A;

[0103] P A—Percentage content of drug A in the mixture, expressed as percentage (%);

[0104] TI B —Toxicity index of drug B;

[0105] P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0106]

[0107] In the formula:

[0108] CTC – Cotoxicity Coefficient;

[0109] ATI – Actual Measured Toxicity Index of Mixtures;

[0110] TTI – Theoretical Toxicity Index of Mixtures.

[0111] The co-toxicity coefficient (CTC) of the compound was ≥120, indicating a synergistic effect; CTC ≤80, indicating an antagonistic effect; and 80 < CTC < 120, indicating an additive effect. The in vitro activity test results are shown below:

[0112] Table 1. Results of the bioactivity assay of compound I in combination with dimpropyridaz on diamondback moth.

[0113]

[0114]

[0115] Table 1 shows that the combination of compound I and dimpropyridaz at an appropriate mass ratio has a significant synergistic effect on diamondback moth. When the mass ratio of compound I to dimpropyridaz is 1:20–35:1, the co-toxicity coefficient against diamondback moth is greater than 120, indicating a synergistic effect in indoor biological activity. When the mass ratio of compound I to dimpropyridaz is 1:10–25:1, the co-toxicity coefficient against diamondback moth is greater than 130, showing a significant synergistic effect; when the mass ratio of compound I to dimpropyridaz is 1:10–15:1, the co-toxicity coefficient against diamondback moth is greater than 140, indicating a remarkable synergistic effect.

[0116] Table 2 shows the results of the bioassay test on the bioactivity of compound I combined with nicofluprole in diamondback moth.

[0117]

[0118] Table 2 shows the results of the indoor experiments. It is evident that compound I and nicofluprole, when combined at appropriate mass ratios, exhibit a significant synergistic effect on diamondback moth. When the mass ratio of compound I to nicofluprole is 1:40–25:1, the co-toxicity coefficient is greater than 120, indicating a synergistic effect in indoor biological activity. When the mass ratio is 1:32–15:1, the co-toxicity coefficient is greater than 130, showing a significant synergistic effect. When the mass ratio is 1:32–8:1, the co-toxicity coefficient on diamondback moth is greater than 140, indicating a substantial synergistic effect.

[0119] Table 3 shows the results of the bioassay test on the bioactivity of compound I with indazapyroxamet in diamondback moth.

[0120]

[0121]

[0122] Table 3 shows the results of the indoor experiments. It is evident that compound I and indazapyroxamet, when combined in an appropriate ratio, exhibit a significant synergistic effect on diamondback moth. When the mass ratio of compound I to indazapyroxamet is 1:50–20:1, the co-toxicity coefficient is greater than 120, indicating a synergistic effect in indoor biological activity. When the mass ratio of compound I to indazapyroxamet is 1:30–15:1, the co-toxicity coefficient is greater than 130, showing a significant synergistic effect. When the mass ratio of compound I to indazapyroxamet is 1:20–10:1, the co-toxicity coefficient is greater than 140, indicating a significant synergistic effect.

[0123] Table 4 shows the results of the indoor bioactivity assay of compound I combined with flumetoquin on diamondback moth.

[0124]

[0125] Table 4 shows the results of the indoor experiments. It is evident that compound I and flumetoquin, when combined in an appropriate ratio, exhibit a significant synergistic effect on diamondback moth. When the mass ratio of compound I to flumetoquin is 1:48–32:1, the co-toxicity coefficient is greater than 120, indicating a synergistic effect in indoor biological activity. When the mass ratio of compound I to flumetoquin is 1:32–16:1, the co-toxicity coefficient is greater than 130, showing a significant synergistic effect. Furthermore, when the mass ratio is 1:16–15:2, the co-toxicity coefficient is greater than 140, demonstrating a remarkable synergistic effect.

[0126] Example 2: Indoor Aphid Bioactivity Test

[0127] The experiment was conducted in accordance with NY / T 1154.14-2008 "Guidelines for Indoor Bioassay of Pesticides - Insecticides - Part 14: Leaf Dipping Method" and NY / T1154.7-2006 "Guidelines for Indoor Bioassay of Pesticides - Insecticides - Part 7: Determination of Combined Effects of Mixtures".

[0128] Experimental target: Peach aphid (Myzus persicae), wingless adult.

[0129] Test reagents: Formula I compound, nicofluprole, dimpropyridaz, frometoquin, and indazapyroxamet technical grade.

[0130] Drug preparation: First, dissolve the original drug in a suitable solvent, then prepare a high-concentration mother liquor. Prepare single-dose mother liquors separately, and design a reasonable ratio according to the purpose of mixing and drug activity. Each single drug and each group of mixed formulations are prepared according to the required series of mass concentrations by the method of equal ratio.

[0131] Experimental Method: Fresh cabbage leaves of similar size and color, untreated, were selected and made into suitable leaf discs using a perforator. The leaf discs were immersed in the test solution for 10 seconds and then placed in a rearing box lined with moisturizing filter paper. Wingless adult aphids of similar size and vigor, raised indoors, were introduced onto the treated leaf discs. Each replicate consisted of 30 aphids, and each treatment concentration was repeated four times. A control group was prepared using a solvent without the same concentration of the pesticide. After treatment, the rearing boxes were placed in an incubator at a temperature of (26±1)℃, relative humidity of (60±5)℃, and a photoperiod of L:D = (16:8)h.

[0132] Data survey: The mortality of test insects was investigated 72 hours after treatment with the pesticide. The criteria for judging the mortality of test insects were obvious shrinkage of the insect body or inability to crawl normally when pricked. The total number of insects and the number of dead insects were recorded.

[0133] Calculation method:

[0134] Calculate the mortality rate for each treatment based on the survey data. Use the following formula:

[0135]

[0136] In the formula:

[0137] P – Mortality rate, expressed as a percentage (%);

[0138] K represents the number of dead insects, in heads;

[0139] N represents the total number of insects treated, in units of heads.

[0140]

[0141] In the formula:

[0142] P1—Adjusted mortality rate, in percentage (%);

[0143] P t —The mortality rate is expressed as a percentage (%).

[0144] P0 – Mortality rate in the blank control group, expressed as a percentage (%).

[0145] If the control mortality rate is <5%, no correction is needed; if the control mortality rate is between 5% and 20%, correction should be performed according to the corrected mortality rate formula; if the control mortality rate is >20%, the trial needs to be repeated.

[0146] The toxicity regression equation and LC were obtained using a statistical analysis system. 50 The values ​​and correlation coefficients are used to evaluate the activity of the test reagent on the biological sample.

[0147] The co-toxicity coefficient (CTC value) of the mixture is calculated using the following formula:

[0148]

[0149] In the formula:

[0150] ATI – Actual Measured Toxicity Index of Mixtures;

[0151] S – LC50 of standard insecticides 50 The unit is milligrams per liter (mg / L);

[0152] M – LC of the mixture 50 The unit is milligrams per liter (mg / L).

[0153] TTI = TI A ×P A +TI B ×P B

[0154] In the formula:

[0155] TTI – Theoretical Toxicity Index of Mixtures;

[0156] TI A —A. Toxicity index of drug A;

[0157] P A —Percentage content of drug A in the mixture, expressed as percentage (%);

[0158] TI B —Toxicity index of drug B;

[0159] P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0160]

[0161] In the formula:

[0162] CTC – Cotoxicity Coefficient;

[0163] ATI – Actual Measured Toxicity Index of Mixtures;

[0164] TTI – Theoretical Toxicity Index of Mixtures.

[0165] The co-toxicity coefficient of the compound is ≥120, which shows a synergistic effect; CTC≤80 shows an antagonistic effect; and 80<CTC<120 shows an additive effect.

[0166] The indoor activity test is shown below:

[0167] Table 5. Results of the bioactivity assay of compound I in combination with dimpropyridaz against aphids.

[0168]

[0169] Table 5 shows that the combination of compound I and dimpropyridaz at an appropriate mass ratio has a significant synergistic effect on aphids. When the mass ratio of compound I to dimpropyridaz is 1:48–48:1, the co-toxicity coefficient against aphids is greater than 120, indicating a synergistic effect in indoor biological activity. When the mass ratio is 1:36–36:1, the co-toxicity coefficient against aphids is greater than 130, showing a significant synergistic effect. When the mass ratio is 1:24–24:1, the co-toxicity coefficient against aphids is greater than 140, indicating a remarkable synergistic effect.

[0170] Table 6 shows the results of the bioassay test on the bioactivity of compound I in combination with nicofluprole against aphids.

[0171]

[0172] Table 6 shows the results of the indoor experiments. It is evident that the combination of compound I and nicofluprole at an appropriate mass ratio exhibits a significant synergistic effect on aphids. When the mass ratio of compound I to nicofluprole is 1:28–42:1, the co-toxicity coefficient is greater than 120, indicating a synergistic effect. When the mass ratio is 1:14–35:1, the co-toxicity coefficient on aphids is greater than 140, showing a significant synergistic effect. When the mass ratio is 1:7–35:1, the co-toxicity coefficient on aphids is greater than 150, indicating a remarkable synergistic effect.

[0173] Table 7. Results of bioassays on the bioactivity of compound I in combination with indazapyroxamet against aphids.

[0174]

[0175] Table 7 shows the results of the indoor experiments. It is evident that the combination of compound I and indazapyroxamet in an appropriate ratio exhibits a significant synergistic effect on aphids. When the mass ratio of compound I to indazapyroxamet is 1:20–25:1, the co-toxicity coefficient is greater than 120, indicating a synergistic effect. When the mass ratio of compound I to indazapyroxamet is 1:15–15:1, the co-toxicity coefficient is greater than 130, showing a significant synergistic effect. When the mass ratio of compound I to indazapyroxamet is 1:5–10:1, the co-toxicity coefficient is greater than 150, indicating a remarkable synergistic effect.

[0176] Table 8. Results of the indoor bioactivity assay of compound I in combination with floctoquin against aphids.

[0177]

[0178] Table 8 shows the results of the indoor experiments. It is evident that compound I and floctoquin, when combined in an appropriate ratio, exhibit a significant synergistic effect on aphids. When the mass ratio of compound I to floctoquin is 1:30–40:1, the co-toxicity coefficient is greater than 120, indicating a synergistic effect. When the mass ratio of compound I to floctoquin is 1:20–30:1, the co-toxicity coefficient is greater than 140, showing a significant synergistic effect. Furthermore, when the mass ratio is 1:20–20:1, the co-toxicity coefficient is greater than 150, demonstrating a remarkable synergistic effect.

[0179] Example 3: Field efficacy test for controlling diamondback moth

[0180] Experimental basis: The experiment referenced GB / T 17980.13-2000 "Guidelines for Field Efficacy Tests of Pesticides (I) Control of Lepidoptera Larvae in Cruciferous Vegetables with Insecticides".

[0181] Experimental site: Cabbage planting area in Qingshui Village, Qingshuiyi Township, Yuzhong County, Lanzhou City, Gansu Province. The soil of the experimental site is black loess, and the fertility of the experimental site is medium to high.

[0182] Experimental target: Diamondback moth.

[0183] Experimental crop: Cabbage (Zhonggan 12).

[0184] Experimental Design: The experiment consisted of 10 treatments arranged in a randomized block design, with each treatment replicated 4 times. The area of ​​each experimental plot was 20 m². 2 .

[0185] Experimental method: The experiment was conducted in April 2023. At the time of application, the cabbage was in its vigorous growth period, and the diamondback moth was mainly composed of young larvae, with a relatively uniform occurrence.

[0186] Survey methods: The initial insect population was assessed before pesticide application, and again at 3, 7, and 14 days after application. Five diagonal sampling points were used, with two plants sampled at each point, to count the number of live insects on the entire cabbage plant. The control effect was calculated based on the survey data.

[0187] Method for calculating the effectiveness of prevention:

[0188]

[0189]

[0190] Results of field efficacy trials:

[0191] Table 9 Results of field efficacy trials for controlling diamondback moth.

[0192]

[0193] Safety results: No phytotoxicity was observed in any of the treatment groups during the field trial, and no adverse effects were found on the surrounding environment or other beneficial organisms in the test area, indicating that the tested agents were safe for cabbage growth at the supplied dosage.

[0194] Table 9 shows the results after 3 days of application of different agents. The control effects of 24% Formula I compound·nicofluprole suspension (1:2), 18% Formula I compound·dimpropyridaz suspension (1:5), 20.4% Formula I compound·flometoquin suspension (2:15), and 1.5% Formula I compound·indazapyroxamet emulsion (1:5) were 86.89%, 87.36%, 85.20%, and 86.57%, respectively, indicating that the insecticidal composition of the present invention has good rapid effect. After 14 days, the control efficacy of 24% Formula I compound·nicofluprole suspension (1:2) was the highest at 98.27%, and the four compound treatment groups showed better sustained efficacy compared with the single-agent control.

[0195] Example 4: Field efficacy trial for controlling aphids

[0196] Experiment location: Strawberry greenhouse in Zhengzhuang Village, Jiawang District, Xuzhou City, Jiangsu Province.

[0197] Experimental target: aphids.

[0198] Experimental crop: Strawberry (Red Beauty).

[0199] Experimental Investigation: The experiment was conducted on March 30, 2023, with one application of pesticide. The foliar spraying was performed using a Gongnong 16-type backpack manual sprayer. Uniform spraying was required, ensuring no overlap or missed areas. A blank control was sprayed with an equal amount of water. Each experimental plot covered an area of ​​30 m². 2 Each treatment was repeated 4 times, and all experimental plots were randomly arranged in blocks.

[0200] Survey Methods: A total of three surveys were conducted throughout the experiment. The initial insect population was surveyed before pesticide application. The remaining insect population was surveyed 3 days and 7 days after application. Five fixed points were used per plot during the survey, with two strawberry seedlings marked at each point. The control efficacy was calculated based on the number of aphids on 10 strawberry seedlings.

[0201] During the experiment, the crops in each treatment area were observed periodically to compare with the blank control to see if any phytotoxicity occurred, and to determine whether the experiment had any adverse effects on the target organisms.

[0202] Methods for calculating drug efficacy:

[0203]

[0204] The field efficacy trials are shown in the table below:

[0205] Table 10 Results of field efficacy trials for controlling aphids

[0206]

[0207] Safety investigation: No phytotoxicity was observed in any of the treatment groups during the field trial, and no adverse effects were found on the surrounding environment or other beneficial organisms in the test area, indicating that the tested agents were safe for strawberry growth at the supplied dosage.

[0208] The results showed (see Table 10) that the efficacy of different agents varied greatly. At 3 and 7 days after application, the rapid and sustained effects of 24% Compound I·nicofluprole suspension (1:2), 27% Compound I·dimpropyridaz water-dispersible granules (8:1), 20.4% Compound I·flometoquin suspension (2:15), and 1.5% Compound I·indazapyroxamet emulsion (1:5) on aphid control were significantly better than those of other agents.

[0209] In summary, through indoor toxicity testing and field efficacy trials, it can be seen that the insecticidal composition of the present invention has a good control effect on lepidopteran and hemiptera pests, and is safe for target crops. It has significant control effect and is superior to single agents in delaying the development of resistance, improving the speed of action and prolonging the duration of action.

[0210] Although this application describes specific embodiments in detail with the aid of examples, the disclosure of this application can be modified and substituted in various ways. However, it should be understood that the disclosure of this application is not limited to the specific form disclosed. Rather, the disclosure of this application covers all modifications, equivalents, and substitutions within the scope of the disclosure of this application, the scope of which is defined by the appended claims and their legal equivalents.

Claims

1. An insecticidal composition and its application, characterized in that, The insecticidal composition comprises active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula I: The active ingredient B is any one of nicofluprole, dimpropyridaz, frometoquin, and indazapyroxamet, and the mass ratio of active ingredient A to active ingredient B is 1:55 to 60:

1.

2. The insecticidal composition according to claim 1, characterized in that, The active ingredient B is nicofluprole, and the mass ratio of the compound of formula I to nicofluprole is 1:40 to 42:1; The active ingredient B is dimpropyridaz, and the mass ratio of the compound of formula I to dimpropyridaz is 1:48 to 48:1; The active ingredient B is flocetoquin, and the mass ratio of the compound of formula I to flocetoquin is 1:48 to 32:1; The active ingredient B is indazapyroxamet, and the mass ratio of the compound of formula I to indazapyroxamet is 1:50 to 20:

1.

3. The insecticidal composition according to claim 2, characterized in that, The active ingredient B is nicofluprole, and the mass ratio of the compound of formula I to nicofluprole is 1:28 to 25:1; The active ingredient B is dimpropyridaz, and the mass ratio of the compound of formula I to dimpropyridaz is 1:36 to 36:1; The active ingredient B is floctoquin, and the mass ratio of the compound of formula I to floctoquin is 1:32 to 32:1; The active ingredient B is indazapyroxamet, and the mass ratio of the compound of formula I to indazapyroxamet is 1:40 to 15:

1.

4. The insecticidal composition according to claim 3, characterized in that, The active ingredient B is nicofluprole, and the mass ratio of the compound of formula I to nicofluprole is 1:16 to 15:1; The active ingredient B is dimpropyridaz, and the mass ratio of the compound of formula I to dimpropyridaz is 1:20 to 35:1; The active ingredient B is floctoquin, and the mass ratio of the compound of formula I to floctoquin is 1:30 to 30:1; The active ingredient B is indazapyroxamet, and the mass ratio of the compound of formula I to indazapyroxamet is 1:15 to 15:

1.

5. The insecticidal composition according to claim 1, characterized in that, The total weight of the insecticidal composition is 100 wt%, and the total weight of active ingredient A and active ingredient B accounts for 0.5 wt% to 80 wt% of the total weight of the insecticidal composition.

6. The insecticidal composition according to claim 1, characterized in that, The insecticidal composition is prepared into an agriculturally permissible formulation, wherein the formulation is a solid formulation or a liquid formulation; The solid formulation is a wettable powder or a water-dispersible granule, and the liquid formulation is any one of a suspension concentrate, emulsifiable concentrate, dispersible oil suspension, water emulsion, or microemulsion.

7. The insecticidal composition according to claim 1, characterized in that, In addition to the active ingredient, the insecticidal composition also includes auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, preservatives, stabilizers, synergists or carriers.

8. The application of the insecticidal composition according to any one of claims 1-7 for the control of plant pests.

9. The application according to claim 8, characterized in that, The plant pests mentioned are lepidopteran pests and hemiptera pests.

10. The application according to claim 8, characterized in that, The plant pests mentioned are diamondback moth and aphid.

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