Application of activated water in promoting crop growth

Abstract

The invention provides an application of activated water in promotion of crop growth, working gas is introduced into a plasma generator for reaction to obtain plasma gas, the plasma gas is introduced into a water body for treatment to obtain plasma activated water, and then the plasma activated water is subjected to light-shielding standing for 10-50 h to obtain the activated water, or the plasma gas and ozone are mixed for reaction and then are introduced into the water body for treatment, and the activated water is obtained. The activated water can effectively reduce the content of peroxynitrous acid, and can promote the growth of crops.

Description

Technical Field

[0001] This invention relates to the field of low-temperature plasma activated water technology, specifically to the application of activated water in promoting crop growth. Background Technology

[0002] Low-temperature plasma activated water (PAW) has been widely studied and reported as a novel disinfectant. For example, Chinese invention patent document CN119430372A discloses a system and method for enhancing the sterilization and disinfection effect of plasma activated water. In the system, an underwater dual-bubble plasma generator is electrically connected to a low-temperature plasma power supply. A pair of bubble reactors are arranged at intervals and are at least partially immersed in a container. A metal rod extends inside a quartz tube via a positioning ring connected to the tube opening. The metal rod is connected to the low-temperature plasma power supply, and a gas channel introduces gas into the quartz tube through the positioning ring. A nitrogen source is connected to one bubble reactor to introduce nitrogen gas through the gas channel, and an argon source is connected to the other bubble reactor to introduce argon gas through the gas channel. When a voltage is applied to the two metal rods of the pair of bubble reactors, the two bubble reactors simultaneously discharge to generate plasma and generate NO2. - and H2O2 active particles, NO2 - It reacts with H2O2 active particles to produce peroxynitrite (ONOO). Based on the chemical pathway of the above system, the sterilization and disinfection function of plasma-activated water is enhanced.

[0003] However, there are few reports on the use of low-temperature plasma-activated water for crop growth because PAW contains peroxynitrite, and the strong oxidizing properties of peroxynitrite cause oxidative damage to crops, which is detrimental to their growth. Summary of the Invention

[0004] The technical problem to be solved by this invention is how to reduce peroxynitrite in plasma-activated water.

[0005] The present invention solves the above-mentioned technical problems through the following technical means:

[0006] This invention provides an application of activated water in promoting crop growth. A working gas is introduced into a plasma generator to react and obtain plasma gas. The plasma gas is then introduced into a water body for treatment to obtain plasma-activated water. The plasma-activated water is then left to stand in the dark for 10-50 hours to obtain the activated water. Alternatively, the plasma gas is mixed with ozone and reacted before being introduced into a water body for treatment to obtain the activated water.

[0007] Beneficial effects: This invention allows plasma-activated water to undergo natural decomposition of peroxynitrite by being left to stand in the dark, thereby reducing the peroxynitrite content. At the same time, the activated water contains nitrate and nitric oxide, which do not change when left to stand in the dark. Therefore, the activated water can be used as a nitrogen fertilizer to promote crop growth.

[0008] This invention activates water by mixing and reacting plasma gas with ozone, which can effectively inhibit the production of peroxynitrite in the activated water and promote the formation of nitrate ions, thereby reducing the peroxynitrite content and further promoting crop growth.

[0009] Preferably, the working gas is one or more of the following: air, a mixture of nitrogen and oxygen, a mixture of air and nitrogen, a mixture of air and oxygen, or a mixture of air, nitrogen and oxygen.

[0010] Preferably, the plasma generator includes one of a dielectric barrier discharge device, a plasma jet device, or a microwave plasma device.

[0011] Preferably, the flow rate of the working gas is 15L / min to 30L / min.

[0012] Preferably, ozone is generated by an ozone generator.

[0013] Preferably, the ozone concentration is 500ppm to 5000ppm.

[0014] Preferably, the ozone flow rate is 5 L / min to 15 L / min.

[0015] Preferably, the processing time is 1 min to 30 min.

[0016] Preferably, the water body is one or more of deionized water, tap water, or natural water sources.

[0017] Preferably, the volume of the water is 50 mL to 1000 mL. Attached Figure Description

[0018] Figure 1 This is a structural diagram of the device used in Embodiment 1 of the present invention; Figure 2 This is a structural diagram of the device used in Embodiment 2 of the present invention; Figure 3 This is a graph showing the peroxynitrite content in the activated water prepared in Example 1 and the plasma-activated water prepared in Comparative Example 1. Figure 4 This is a graph showing the peroxynitrite content in the activated water prepared in Example 2 of the present invention and the plasma-activated water prepared in Comparative Example 1. Figure 5 This is a graph showing the nitrate content in the activated water prepared in Example 1 and the plasma-activated water prepared in Comparative Example 1. Figure 6 This is a graph showing the nitrate content in the activated water prepared in Example 2 of the present invention and the plasma-activated water prepared in Comparative Example 1. Figure 7 The content of nitric oxide in the activated water prepared in Example 1 of this invention and the plasma activated water prepared in Comparative Example 1 is shown. Figure 8 This is a graph showing the nitric oxide content in the activated water prepared in Example 2 of the present invention and the plasma-activated water prepared in Comparative Example 1. Figure 9 The effects of activated water prepared in Examples 1-2 and plasma-activated water prepared in Comparative Example 1 on the growth of wheat seedlings are described. Figure 10 The effects of activated water prepared in Examples 1-2 and plasma-activated water prepared in Comparative Example 1, combined with Hogrange solution, on wheat seedling growth; Figure 11 The effects of activated water prepared in Examples 1-2 and plasma-activated water prepared in Comparative Example 1 on the growth of wheat seedlings in pots are described. Detailed Implementation

[0019] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0020] Unless otherwise specified, all test materials and reagents used in the following examples are commercially available.

[0021] Unless otherwise specified in the embodiments, the techniques or conditions described in the literature in this field or in accordance with the product manual may be followed.

[0022] Example 1 This embodiment provides an application of activated water in promoting crop growth, the method of which is as follows: Air is introduced into a plasma jet device (manufactured by Zhengzhou Chuangtu Power Supply Co., Ltd.) to react and obtain plasma gas, wherein the discharge power of the plasma jet device is 140W; then the plasma gas is mixed with ozone and reacted, and then introduced into water for activation treatment to obtain activated water, denoted as PAW1; the water is 150mL of deionized water.

[0023] The specific steps are as follows: According to Figure 1 As shown, both the plasma jet device and the ozone reactor are connected to the gas reaction chamber, which is in turn connected to a water body. An air compressor introduces air into the plasma jet device at a rate of 15 L / min to produce plasma gas. An oxygen cylinder introduces oxygen into the ozone reactor to produce ozone. The ozone concentration is 2500 ppm, and the ozone flow rate is 5 L / min. After the plasma gas and ozone are mixed and reacted in the gas reaction chamber, the mixture is introduced into a water body (150 mL of deionized water) for activation treatment, resulting in activated water.

[0024] Different activated water (PAW1) is obtained depending on the activation treatment time. For example, activation treatment of 1 min is recorded as PAW1-1 min, activation treatment of 2 min is recorded as PAW1-2 min, and activation treatment of 3 min is recorded as PAW1-3 min.

[0025] Example 2 This embodiment provides an application of activated water in promoting crop growth, the method of which is as follows: Air is introduced into a plasma jet device (manufactured by Zhengzhou Chuangtu Power Supply Co., Ltd.) to react and obtain plasma gas, wherein the discharge power of the plasma jet device is 140W; the plasma gas is introduced into water for activation treatment to obtain plasma activated water, and then the plasma activated water is left to stand in the dark for 24 hours to obtain the activated water, denoted as PAW2; the water is 150mL of deionized water. The specific steps are as follows: According to Figure 2 As shown, the plasma jet device is connected to a water body (deionized water), which is connected to a light-proof settling box via a pipeline. The settling box contains a container (not shown) holding the water. An air compressor introduces air into the plasma jet device at a rate of 15 L / min to produce plasma gas. This plasma gas is then introduced into the water body (150 mL of deionized water) for activation treatment to obtain plasma-activated water. The plasma-activated water is then settling in the settling box in the dark for 24 hours to obtain the activated water (PAW2).

[0026] Different activated water (PAW2) is obtained depending on the activation treatment time. For example, activation treatment of 1 min is recorded as PAW2-1 min, activation treatment of 2 min is recorded as PAW2-2 min, and activation treatment of 3 min is recorded as PAW2-3 min.

[0027] Comparative Example 1 This comparative example provides plasma-activated water. The difference between this comparative example and Example 2 is that no light-protected standing treatment was performed, resulting in plasma-activated water (PAW), which is designated as the control.

[0028] Different activated water (PAW) is obtained depending on the activation treatment time. For example, activation treatment of 1 min is recorded as PAW-1 min, activation treatment of 2 min is recorded as PAW-2 min, and activation treatment of 3 min is recorded as PAW-3 min.

[0029] Experimental Example The contents of peroxynitrite, nitrate, and nitric oxide in PAW, PAW1, and PAW2 were determined, respectively, using the following methods: 1. The content of peroxynitrite is detected by electron spin resonance probe microanalysis, specifically as follows: Figure 3 and Figure 4 As shown, according to Figure 3 As shown in the figure, the time represents the activation treatment time. The peroxynitrite content in PAW1 is significantly lower than that in PAW, indicating that PAW1 effectively reduces the peroxynitrite content. According to... Figure 4 As shown, the peroxynitrite content in PAW2-3min was significantly lower than that in PAW-3min, indicating that PAW2 effectively reduced the peroxynitrite content.

[0030] 2. The nitrate content is detected by ultraviolet spectrophotometry, specifically as follows: Figure 5 and Figure 6 As shown, according to Figure 5 As shown, the nitrate content in PAW1 is higher than that in PAW, and with the increase of activation time, the nitrate content in PAW1 is significantly higher than that in PAW, indicating that PAW1 effectively increases the nitrate content. According to... Figure 6 As shown, the nitrate content in PAW increases with increasing activation time, and the nitrate content in PAW2 increases with increasing standing time, indicating that PAW2 can increase the nitrate content.

[0031] 3. Nitric oxide content is detected using an electron spin resonance probe, specifically as follows: Figure 7 and Figure 8 As shown, according to Figure 7 As shown, a slight change in nitric oxide content was observed in PAW1 over 3 minutes, indicating that PAW1 reduced peroxynitrite without significantly affecting nitric oxide content. According to... Figure 8 As shown, the nitric oxide content in PAW2-3 min did not change significantly, indicating that PAW2 reduced peroxynitrite without significantly affecting the nitric oxide content.

[0032] Application examples The activated water prepared in Examples 1 and 2 and the plasma-activated water prepared in Comparative Example 1 were applied to the growth of wheat seedlings. The control group consisted of wheat seedlings grown in a normal environment without the addition of sodium chloride solution; the sodium chloride group represented wheat seedlings grown under salt stress, where 120 mM sodium chloride solution was added to the petri dish or soil. The specific method is as follows: 1. Water the wheat seedlings daily with activated water or plasma-activated water, and observe their growth. Specifically... Figure 9 As shown, Figure 9 This is the growth status on the 9th day after watering.

[0033] 2. Irrigate the wheat seedlings daily with activated water or plasma-activated water and Hoagland solution, and observe the growth status of the wheat seedlings. Specifically, as follows... Figure 10 As shown, Figure 10 This is the growth status on the 9th day after watering.

[0034] 3. Water the potted wheat seedlings daily with activated water or plasma-activated water, and observe their growth. (Specific details are omitted as they are not relevant to the main text.) Figure 11 As shown, Figure 11 This is the growth status on the 9th day after watering.

[0035] according to Figures 9-11 It was found that the wheat seedlings in the control group grew well, while the wheat seedlings in the sodium chloride group showed inhibited growth. This invention, by irrigating the wheat seedlings in the sodium chloride group with activated water, discovered that PAW1 and PAW2 significantly promoted wheat seedling growth, while irrigating the wheat seedlings in the sodium chloride group with plasma-activated water resulted in PAW containing a higher content of peroxynitrite, thus inhibiting wheat growth. Therefore, PAW1 and PAW2 can significantly promote wheat seedling growth under salt stress; while PAW, due to its higher content of peroxynitrite, inhibits wheat growth.

[0036] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. An application of activated water in promoting crop growth, characterized in that, The working gas is introduced into a plasma generator to react and obtain plasma gas. The plasma gas is then introduced into a water body for activation treatment to obtain plasma-activated water. The plasma-activated water is then left to stand in the dark for 10 to 50 hours to obtain the activated water. Alternatively, the plasma gas is mixed with ozone and reacted before being introduced into a water body for activation treatment to obtain the activated water.

2. The application of activated water in promoting crop growth according to claim 1, characterized in that, The working gas is one or more of the following: air, a mixture of nitrogen and oxygen, a mixture of air and nitrogen, a mixture of air and oxygen, or a mixture of air, nitrogen and oxygen.

3. The application of activated water in promoting crop growth according to claim 1, characterized in that, Plasma generators include one of dielectric barrier discharge devices, plasma jet devices, or microwave plasma devices.

4. The application of activated water in promoting crop growth according to claim 1, characterized in that, The working gas flow rate is 15L / min to 30L / min.

5. The application of activated water according to claim 1 in promoting crop growth, characterized in that, Ozone is produced by an ozone generator.

6. The application of activated water according to claim 1 or 5 in promoting crop growth, characterized in that, The concentration of ozone is 500ppm to 5000ppm.

7. The application of activated water according to claim 1 or 5 in promoting crop growth, characterized in that, The ozone flow rate is 5 L / min to 15 L / min.

8. The application of activated water according to claim 1 in promoting crop growth, characterized in that, The activation treatment time is 1 min to 30 min.

9. The application of activated water according to claim 1 in promoting crop growth, characterized in that, The water body is one or more of the following: deionized water, tap water, or natural water source.

10. The application of activated water according to claim 1 in promoting crop growth, characterized in that, The volume of the water is 50mL to 1000mL.

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