Silicon ecological enzyme agricultural preparation with dual functions of biological activation and physical protection and preparation method of silicon ecological enzyme agricultural preparation

Abstract

The invention relates to the technical field of biological agriculture, and discloses a silicon ecological enzyme agricultural preparation with dual functions of biological activation and physical protection and a preparation method of the silicon ecological enzyme agricultural preparation. The invention relates to a preparation method of a silicon ecological enzyme agricultural preparation with biological activation and physical protection dual functions, which comprises the following steps: 1) adding a fermentation supplement into organic wastes to obtain a fermentation raw material, then adding a compound microbial agent into the fermentation raw material, and carrying out sectional fermentation to obtain a fermentation liquid; (2) adding a compound enzyme preparation into the fermentation liquor obtained in the step (1), and performing enzymolysis to obtain enzymatic hydrolysate; 3, a silicon source is supplemented into the enzymatic hydrolysate obtained in the step 2, and the silicon ecological enzyme agricultural preparation with the biological activation and physical protection double functions is obtained.The silicon ecological enzyme agricultural preparation with the biological activation and physical protection double functions has the good soil environment improving effect and the good crop performance improving effect.

Description

Technical Field

[0001] This invention relates to the field of bio-agricultural technology, and more specifically, to a silicon-based ecological enzyme agricultural preparation with dual functions of bioactivation and physical protection, and its preparation method. Background Technology

[0002] With the increasing demands for sustainable development in modern agriculture, the development of bio-agricultural agents using organic waste has become an important direction for resource recycling. Traditional organic fertilizers or microbial fertilizers mainly improve soil fertility by supplementing organic matter and beneficial bacteria, but their function is relatively singular and their effect on stimulating the crop's own potential is limited.

[0003] Silicon has been proven to be a beneficial element required by many crops. Applying silicon can strengthen crop cell walls, forming a physical barrier and thus improving the crop's resistance to pests, diseases, and adverse conditions. However, single silicon fertilizer products often lack biological activity and have insufficient effects on soil improvement and crop growth stimulation.

[0004] Currently, there have been attempts to simply mix organic matter with silicon fertilizer, but due to the differences in their properties, it is difficult to form a stable and synergistic system, resulting in poor effects. Therefore, there is an urgent need in this field for a new type of agricultural formulation that can organically combine the bioactivity of organic waste with the physical protective function of silicon to achieve synergistic effects. Summary of the Invention

[0005] The purpose of this invention is to provide a silicon-based ecological enzyme agricultural preparation with dual functions of bioactivation and physical protection, and its preparation method.

[0006] To achieve the above objectives, the present invention provides the following technical solution: One of the technical solutions of this invention: A method for preparing a silicon-based ecological enzyme agricultural preparation with dual functions of bioactivation and physical protection includes the following steps: 1) Add the fermentation supplement to the organic waste to obtain the fermentation raw material, then add the compound microbial agent to the fermentation raw material and ferment in stages to obtain the fermentation liquid; 2) Add the compound enzyme preparation to the fermentation broth obtained in step 1), and perform enzymatic hydrolysis to obtain the hydrolysate; 3) Add silicon source to the enzymatic hydrolysate obtained in step 2) to obtain the silicon ecological enzyme agricultural preparation with dual functions of bioactivation and physical protection.

[0007] Further, in step 1), the fermentation supplement is a mixture of humic acid, seaweed extract, bentonite and chitin in a mass ratio of (4-6):(2-4):(0.5-1.5):(0.5-1.5).

[0008] Further, in step 1), the method for preparing the organic waste includes the following steps: mixing agricultural waste and livestock manure, and adjusting the C / N ratio to 30:1 to obtain a premix, and then subjecting the premix to physical-chemical synergistic treatment to obtain the organic waste.

[0009] Furthermore, the physical-chemical synergistic treatment specifically involves controlling the ultrasonic frequency at 40kHz and the power at 300W, ultrasonically treating the premix for 15–25 minutes, and then adding 0.5%wt of rhamnolipid and mixing thoroughly.

[0010] Further, in step 1), the mass ratio of the fermentation supplement to the organic waste is 1:(30-40).

[0011] Furthermore, in step 1), after adding the fermentation supplement to the organic waste, the pH needs to be adjusted to 6.5 using a pH adjuster and the water content needs to be adjusted to 60% using water.

[0012] Further, in step 1), the compound microbial agent is composed of Bacillus subtilis, thermophilic methanococcus, Bacillus megaterium and azotobacter chrysogenum in a mass ratio of (5-7):(3-5):(2-4):(1-3).

[0013] Further, in step 1), the mass ratio of the fermentation raw material to the compound microbial agent is 50:(1-3).

[0014] Further, in step 1), the segmented fermentation specifically refers to: Aerobic start-up stage: In an environment with an oxygen content of 20%, control the stirring speed at 60 r / min and ferment at a constant temperature of 38-42℃ for 12-36 h; Anaerobic fermentation stage: ferment at a constant temperature of 45-48℃ for 4-6 days in an environment with oxygen content ≤1%. During this period, the stirring speed is controlled at 50r / min and stirred for 15min every 12h. Microaerobic ripening stage: Ferment at a constant temperature of 35-37℃ for 5-7 days in an environment with 5% oxygen content. During this period, the stirring speed is controlled at 30r / min and stirred for 5min every 24 hours.

[0015] Further, in step 2), the compound enzyme preparation is composed of cellulase, protease, hemicellulase and pectinase mixed in a mass ratio of (2-4):(2-4):(1-3):(0.5-1.5).

[0016] Further, in step 2), the ratio of the compound enzyme preparation to the fermentation broth is 1g:(20-30)mL.

[0017] Further, in step 2), the enzymatic hydrolysis specifically includes the following steps: controlling the temperature at 40-45℃ and the pH at 6.5, and enzymatically hydrolyzing in an environment with an oxygen content of 10% for 48-60 hours.

[0018] Furthermore, in step 2), after the enzymatic hydrolysis, the hydrolysate needs to be inactivated, specifically by incubating at 85°C for 30 minutes.

[0019] Further, in step 3), the supplementary silicon source specifically includes the following steps: 1) Add soluble silicon to the enzymatic hydrolysate, mix well, then add 0.3%wt EDTA-2Na, react to obtain soluble silicon-enzymatic hydrolysate; 2) Add the slow-release silicon to the soluble silicon-enzyme hydrolysate obtained in step 1), mix well, sonicate, add 0.1% genipin, crosslink, and obtain the silicon ecological enzyme agricultural preparation.

[0020] Furthermore, in step 1), the soluble silicon is potassium silicate.

[0021] Furthermore, in step 1), the reaction specifically involves adjusting the pH to 7.0, controlling the stirring speed to 80 r / min, and stirring the reaction at 30–40°C for 30 min.

[0022] Furthermore, in step 2), the slow-release silicon is wollastonite powder.

[0023] Furthermore, in step 2), the ultrasonic treatment specifically involves controlling the ultrasonic power to 200W and performing ultrasonic treatment for 15 minutes.

[0024] Furthermore, in step 2), the crosslinking specifically involves controlling the stirring speed to 80 r / min and crosslinking at 50°C for 40–60 min.

[0025] The second technical solution of this invention: The above-mentioned method for preparing a silicon-based ecological enzyme agricultural preparation with dual functions of bioactivation and physical protection yields a silicon-based ecological enzyme agricultural preparation with dual functions of bioactivation and physical protection.

[0026] Compared with the prior art, the beneficial effects of the present invention are as follows: The present invention provides a silicon-based ecological enzyme agricultural preparation with dual functions of bioactivation and physical protection, which can effectively reduce soil salinity, increase organic matter content, soil pH, ammonium nitrogen content, available phosphorus content and available potassium content, and has a good soil environment improvement effect. The present invention provides a silicon-based ecological enzyme agricultural preparation with dual functions of bioactivation and physical protection, which can effectively improve crop yield per acre and root health index and reduce the incidence of diseases and pests, and has a good effect on improving crop performance.

[0027] This invention provides a silicon-based ecological enzyme agricultural preparation with dual functions of bioactivation and physical protection. Its dual-function characteristic is achieved through the following principle: The bioactivation function of the formulation stems from the efficient biotransformation of organic waste. Through the segmented fermentation of compound microorganisms and the targeted enzymatic hydrolysis of compound enzymes, the large organic molecules in the waste are transformed into bioactive substances such as humic acid, amino acids, and small peptides that are easily absorbed by crops. These components can directly stimulate crop growth and development, improve root vitality and nutrient absorption efficiency, and activate crop potential from a physiological level. The physical protection function of the formulation is achieved by constructing a dual-mode silicon source system of "rapid-acting and slow-release"; soluble silicon provides immediate protection, while slow-release silicon ensures long-term supply, and the silicon source and enzymatic active liquid are stably combined through genipin crosslinking technology; after crop absorption, silicon elements form a silicified layer in the epidermal cell wall, which significantly enhances stem strength and leaf toughness, effectively resisting the invasion of pests and diseases and the risk of lodging. In addition, bioactive substances promote the absorption and translocation of silicon by crops, while the physical protection of silicon creates better growth conditions for crops, enabling them to respond more fully to biostimuli, and ultimately play a synergistic role in increasing yield, stress resistance and quality improvement. Detailed Implementation

[0028] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention. It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the present invention.

[0029] Furthermore, regarding the numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Every smaller range between any stated value or intermediate value within a stated range, and any other stated value or intermediate value within said range, is also included in this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0030] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.

[0031] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be apparent to those skilled in the art. This specification and embodiments are merely exemplary.

[0032] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.

[0033] In the following examples, all raw materials used were commercially available, as detailed in Table 1. Table 1 Raw Materials and Specifications

[0034] The following embodiments illustrate a method for preparing a silicon-based ecological enzyme agricultural preparation with dual functions of bioactivation and physical protection, comprising the following steps: 1) The humic acid, seaweed extract, bentonite and chitin were mixed in a mass ratio of (4-6):(2-4):(0.5-1.5):(0.5-1.5) to obtain a fermentation supplement. 2) Mix agricultural waste and livestock manure at a mass ratio of (3-5):1, and adjust the C / N ratio to 30:1 using rice husk powder and urea to obtain a premix. Then, control the ultrasonic frequency at 40kHz and the power at 300W to ultrasonically treat the premix for 15-25 minutes. Finally, add 0.5%wt of rhamnolipin and mix well to obtain organic waste. 3) Add the fermentation supplement obtained in step 1) to the organic waste obtained in step 2) according to the mass ratio of fermentation supplement to organic waste of 1:(30-40), adjust the pH to 6.5 using a pH adjuster (food grade citric acid or calcium hydroxide), and adjust the water content to 60% using water to obtain fermentation raw materials; 4) According to the mass ratio of Bacillus subtilis, thermophilic methanococcus, Bacillus megaterium and azotobacter brownii, Bacillus subtilis, thermophilic methanococcus, Bacillus megaterium and azotobacter brownii are (5-7):(3-5):(2-4):(1-3), Bacillus subtilis, thermophilic methanococcus, Bacillus megaterium and azotobacter brownii are mixed to obtain a compound microbial agent; 5) According to the mass ratio of fermentation raw materials to compound microbial agents of 50:(1-3), add the compound microbial agents obtained in step 4) to the fermentation raw materials obtained in step 3), carry out segmented fermentation, filter under pressure, and obtain fermentation broth; Specifically, the segmented fermentation process includes: Aerobic start-up stage: In an environment with an oxygen content of 20%, control the stirring speed at 60 r / min and ferment at a constant temperature of 38-42℃ for 12-36 h; Anaerobic fermentation stage: ferment at a constant temperature of 45-48℃ for 4-6 days in an environment with oxygen content ≤1%. During this period, the stirring speed is controlled at 50r / min and stirred for 15min every 12h. Microaerobic ripening stage: Ferment at a constant temperature of 35-37℃ for 5-7 days in an environment with 5% oxygen content. During this period, the stirring speed is controlled at 30r / min and stirred for 5min every 24 hours. 6) Mix cellulase, protease, hemicellulase and pectinase in a mass ratio of (2-4):(2-4):(1-3):(0.5-1.5) to obtain a compound enzyme preparation; 7) According to the material-liquid ratio of compound enzyme preparation to fermentation broth of 1g: (20-30)mL, add the compound enzyme preparation obtained in step 6) to the fermentation broth obtained in step 5), control the temperature at 40-45℃ and pH at 6.5, and enzymatically hydrolyze in an environment with 10% oxygen content for 48-60h, and then keep warm at 85℃ for 30min to obtain the enzymatic hydrolysate; 8) Add soluble silicon to the enzymatic hydrolysate obtained in step 7) according to the material-to-liquid ratio of soluble silicon to enzymatic hydrolysate of 1g:(10-20)mL, mix well, then add 0.3%wt EDTA-2Na, adjust the pH to 7.0, control the stirring speed to 80r / min, and stir the reaction at 30-40℃ for 30min to obtain soluble silicon-enzymatic hydrolysate; The soluble silicon is potassium silicate; 9) According to the material-liquid ratio of slow-release silicon to soluble silicon-enzyme hydrolysate of 1g:(20-40)mL, add slow-release silicon to the soluble silicon-enzyme hydrolysate obtained in step 8), mix well, then control the ultrasonic power to 200W, ultrasonically treat for 15min, finally add 0.1% genipin, control the stirring speed to 80r / min, crosslink at 50℃ for 40-60min, and obtain the silicon ecological enzyme agricultural preparation; The slow-release silicon is wollastonite powder.

[0035] Example 1 A silicon-based ecological enzyme agricultural preparation 1) Mix humic acid, seaweed extract, bentonite and chitin in a mass ratio of 5:3:1:1 to obtain a fermentation supplement. 2) Agricultural waste and livestock manure were mixed at a mass ratio of 4:1. The C / N ratio was adjusted to 30:1 using rice husk powder and urea to obtain a premix. The premix was then ultrasonically treated for 20 minutes at a frequency of 40kHz and a power of 300W. Finally, 0.5%wt of rhamnolipid was added and mixed well to obtain organic waste. 3) Add the fermentation supplement obtained in step 1) to the organic waste obtained in step 2) according to the mass ratio of fermentation supplement to organic waste of 1:35, adjust the pH to 6.5 using a pH adjuster (food grade citric acid or calcium hydroxide), and adjust the water content to 60% using water to obtain fermentation raw materials; 4) According to the mass ratio of Bacillus subtilis, thermophilic methanococcus, Bacillus megaterium and azotobacter brownii, Bacillus subtilis, thermophilic methanococcus, Bacillus megaterium and azotobacter brownii were 6:4:3:2, Bacillus subtilis, thermophilic methanococcus, Bacillus megaterium and azotobacter brownii were mixed to obtain a compound microbial agent; 5) According to the mass ratio of fermentation raw materials to compound microbial inoculant of 50:2, add the compound microbial inoculant obtained in step 4) to the fermentation raw materials obtained in step 3), carry out segmented fermentation, filter by pressure, and obtain fermentation broth; Specifically, the segmented fermentation process includes: Aerobic start-up stage: In an environment with an oxygen content of 20%, the stirring speed is controlled at 60 r / min, and the fermentation is carried out at a constant temperature of 40℃ for 18 hours; Anaerobic fermentation stage: fermentation at 46℃ for 5 days in an environment with oxygen content ≤1%, during which the stirring speed is controlled at 50r / min and stirred for 15min every 12h; Microaerobic ripening stage: fermentation at 36℃ for 6 days in an environment with 5% oxygen content. During this period, the stirring speed is controlled at 30r / min and stirred for 5min every 24h. 6) Mix cellulase, protease, hemicellulase and pectinase in a mass ratio of 3:3:2:1 to obtain a compound enzyme preparation; 7) According to the material-liquid ratio of compound enzyme preparation to fermentation broth of 1g:25mL, add the compound enzyme preparation obtained in step 6) to the fermentation broth obtained in step 5), control the temperature at 42℃ and pH at 6.5, and enzymatically hydrolyze for 54h in an environment with 10% oxygen content, and then keep warm at 85℃ for 30min to obtain the enzymatic hydrolysate. 8) Add soluble silicon to the enzymatic hydrolysate obtained in step 7) according to the material-to-liquid ratio of soluble silicon to enzymatic hydrolysate of 1g:15mL, mix well, then add 0.3%wt EDTA-2Na, adjust the pH to 7.0, control the stirring speed to 80r / min, and stir the reaction at 35℃ for 30min to obtain soluble silicon-enzymatic hydrolysate; The soluble silicon is potassium silicate; 9) According to the material-liquid ratio of slow-release silicon to soluble silicon-enzyme hydrolysate of 1g:30mL, add slow-release silicon to the soluble silicon-enzyme hydrolysate obtained in step 8), mix well, then control the ultrasonic power to 200W, ultrasonically treat for 15min, finally add 0.1% genipin, control the stirring speed to 80r / min, crosslink at 50℃ for 50min, and obtain the silicon ecological enzyme agricultural preparation; The slow-release silicon is wollastonite powder.

[0036] Example 2 A silicon-based ecological enzyme agricultural preparation 1) The fermentation supplement is prepared by mixing humic acid, seaweed extract, bentonite and chitin in a mass ratio of 4:2:0.5:0.5. 2) Agricultural waste and livestock manure were mixed at a mass ratio of 3:1. The C / N ratio was adjusted to 30:1 using rice husk powder and urea to obtain a premix. The premix was then ultrasonically treated for 15 minutes at a frequency of 40 kHz and a power of 300 W. Finally, 0.5% wt of rhamnolipid was added and mixed well to obtain organic waste. 3) Add the fermentation supplement obtained in step 1) to the organic waste obtained in step 2) according to the mass ratio of fermentation supplement to organic waste of 1:30, adjust the pH to 6.5 using a pH adjuster (food-grade citric acid or calcium hydroxide), and adjust the water content to 60% using water to obtain fermentation raw materials; 4) Bacillus subtilis, thermophilic methanococcus, Bacillus megaterium, and azotobacter brownii were mixed in a mass ratio of 5:3:2:1 to obtain a compound microbial agent. 5) Add the compound microbial agent obtained in step 4) to the fermentation raw material obtained in step 3) according to the mass ratio of fermentation raw material to compound microbial agent of 50:1, carry out segmented fermentation, filter by pressure, and obtain fermentation broth; Specifically, the segmented fermentation process includes: Aerobic start-up stage: In an environment with an oxygen content of 20%, the stirring speed is controlled at 60 r / min, and the fermentation is carried out at a constant temperature of 38℃ for 12 hours; Anaerobic fermentation stage: fermentation at 45℃ for 4 days in an environment with oxygen content ≤1%, during which the stirring speed is controlled at 50r / min and stirred for 15min every 12h; Microaerobic ripening stage: Ferment at 35℃ for 5 days in an environment with 5% oxygen content. During this period, the stirring speed is controlled at 30r / min and stirred for 5min every 24 hours. 6) Mix cellulase, protease, hemicellulase and pectinase in a mass ratio of 2:2:1:0.5 to obtain a compound enzyme preparation; 7) According to the material-liquid ratio of compound enzyme preparation to fermentation broth of 1g:20mL, add the compound enzyme preparation obtained in step 6) to the fermentation broth obtained in step 5), control the temperature at 40℃ and pH at 6.5, and enzymatically hydrolyze for 48h in an environment with 10% oxygen content, and then keep warm at 85℃ for 30min to obtain the enzymatic hydrolysate. 8) Add soluble silicon to the enzymatic hydrolysate obtained in step 7) according to the material-to-liquid ratio of soluble silicon to enzymatic hydrolysate of 1g:10mL, mix well, then add 0.3%wt EDTA-2Na, adjust the pH to 7.0, control the stirring speed to 80r / min, and stir the reaction at 30℃ for 30min to obtain soluble silicon-enzymatic hydrolysate; The soluble silicon is potassium silicate; 9) According to the material-liquid ratio of slow-release silicon to soluble silicon-enzyme hydrolysate of 1g:20mL, add slow-release silicon to the soluble silicon-enzyme hydrolysate obtained in step 8), mix well, then control the ultrasonic power to 200W, ultrasonically treat for 15min, finally add 0.1% genipin, control the stirring speed to 80r / min, crosslink at 50℃ for 40min, and obtain the silicon ecological enzyme agricultural preparation; The slow-release silicon is wollastonite powder.

[0037] Example 3 A silicon-based ecological enzyme agricultural preparation 1) The fermentation supplement is prepared by mixing humic acid, seaweed extract, bentonite and chitin in a mass ratio of 6:4:1.5:1.5. 2) Agricultural waste and livestock manure were mixed at a mass ratio of 5:1. The C / N ratio was adjusted to 30:1 using rice husk powder and urea to obtain a premix. The premix was then ultrasonically treated for 25 minutes at a frequency of 40 kHz and a power of 300 W. Finally, 0.5% wt of rhamnolipid was added and mixed well to obtain organic waste. 3) Add the fermentation supplement obtained in step 1) to the organic waste obtained in step 2) according to the mass ratio of fermentation supplement to organic waste of 1:40, adjust the pH to 6.5 using a pH adjuster (food-grade citric acid or calcium hydroxide), and adjust the water content to 60% using water to obtain fermentation raw materials; 4) According to the mass ratio of Bacillus subtilis, thermophilic methanococcus, Bacillus megaterium and azotobacter brownii, Bacillus subtilis, thermophilic methanococcus, Bacillus megaterium and azotobacter brownii were 7:5:4:3, Bacillus subtilis, thermophilic methanococcus, Bacillus megaterium and azotobacter brownii were mixed to obtain a compound microbial agent. 5) According to the mass ratio of fermentation raw materials to compound microbial inoculant of 50:3, add the compound microbial inoculant obtained in step 4) to the fermentation raw materials obtained in step 3), carry out segmented fermentation, filter by pressure, and obtain fermentation broth; Specifically, the segmented fermentation process includes: Aerobic start-up stage: In an environment with an oxygen content of 20%, the stirring speed is controlled at 60 r / min, and the fermentation is carried out at a constant temperature of 42℃ for 36 hours; Anaerobic fermentation stage: fermentation at 48℃ for 6 days in an environment with oxygen content ≤1%, during which the stirring speed is controlled at 50r / min and stirred for 15min every 12h; Microaerobic ripening stage: fermentation at 37℃ for 7 days in an environment with 5% oxygen content. During this period, the stirring speed is controlled at 30r / min and stirred for 5min every 24h. 6) Mix cellulase, protease, hemicellulase and pectinase in a mass ratio of 4:4:3:1.5 to obtain a compound enzyme preparation. 7) According to the material-liquid ratio of compound enzyme preparation to fermentation broth of 1g:30mL, add the compound enzyme preparation obtained in step 6) to the fermentation broth obtained in step 5), control the temperature at 45℃ and pH at 6.5, and enzymatically hydrolyze for 60h in an environment with 10% oxygen content, and then keep warm at 85℃ for 30min to obtain the enzymatic hydrolysate. 8) Add soluble silicon to the enzymatic hydrolysate obtained in step 7) according to the material-to-liquid ratio of soluble silicon to enzymatic hydrolysate of 1g:20mL, mix well, then add 0.3%wt EDTA-2Na, adjust the pH to 7.0, control the stirring speed to 80r / min, and stir the reaction at 40℃ for 30min to obtain soluble silicon-enzymatic hydrolysate; Wherein, the soluble silicon is potassium silicate; 9) According to the material-liquid ratio of slow-release silicon to soluble silicon-enzyme hydrolysate of 1g:40mL, add slow-release silicon to the soluble silicon-enzyme hydrolysate obtained in step 8), mix well, then control the ultrasonic power to 200W, ultrasonically treat for 15min, finally add 0.1% genipin, control the stirring speed to 80r / min, crosslink at 50℃ for 60min, and obtain the silicon ecological enzyme agricultural preparation; The slow-release silicon is wollastonite powder.

[0038] Comparative Example 1 An agricultural preparation Same as Example 1, except that step 1) is: The fermentation supplement is obtained by mixing humic acid, bentonite, and chitin in a mass ratio of 5:1:1.

[0039] Comparative Example 2 An agricultural preparation Same as Example 1, except that step 1) is: The fermentation supplement was prepared by mixing humic acid, seaweed extract and bentonite in a mass ratio of 5:3:1.

[0040] Comparative Example 3 An agricultural preparation Same as Example 1, except that step 2) is: Agricultural waste and livestock manure were mixed at a mass ratio of 4:1, and the C / N ratio was adjusted to 30:1 using rice husk powder and urea to obtain a premix. The premix was then ultrasonically treated for 20 minutes at a frequency of 40kHz and a power of 300W to obtain organic waste.

[0041] Comparative Example 4 An agricultural preparation Same as Example 1, except that step 2) is: Agricultural waste and livestock manure were mixed at a mass ratio of 4:1, and the C / N ratio was adjusted to 30:1 using rice husk powder and urea to obtain a premix. Then, 0.5% wt of rhamnolipin was added to the premix and mixed well to obtain organic waste.

[0042] Comparative Example 5 An agricultural preparation Same as Example 1, except that step 4) is: A compound microbial agent was obtained by mixing Bacillus subtilis, Bacillus megaterium, and Azotobacter chrysogenum in a mass ratio of 6:3:2.

[0043] Comparative Example 6 An agricultural preparation Same as Example 1, except that step 4) is: A compound microbial agent was obtained by mixing Bacillus subtilis, thermophilic methanococcus, and Bacillus megaterium in a mass ratio of 6:4:3.

[0044] Comparative Example 7 An agricultural preparation Same as Example 1, except that step 5) is: The fermentation raw material and the compound microbial agent were added to the fermentation raw material obtained in step 3) at a mass ratio of 50:2. The mixture was then fermented at 40°C for 12 days in an environment with an oxygen content of 20% while the stirring speed was controlled at 60 r / min. The mixture was then filtered to obtain the fermentation broth.

[0045] Comparative Example 8 An agricultural preparation Same as Example 1, except that steps 6) and 7) are omitted, and the silicon source is directly added to the fermentation broth.

[0046] Comparative Example 9 An agricultural preparation Same as Example 1, except that step 8) is changed to: According to the material-liquid ratio of soluble silicon to enzymatic hydrolysate of 1g:15mL, soluble silicon is added to the enzymatic hydrolysate obtained in step 7), and mixed well to obtain soluble silicon-enzymatic hydrolysate. The soluble silicon is potassium silicate.

[0047] Comparative Example 10 An agricultural preparation Same as Example 1, except that step 9) is changed to: The slow-release silicon and soluble silicon-enzyme hydrolysate were mixed at a ratio of 1g:30mL. The slow-release silicon was added to the soluble silicon-enzyme hydrolysate obtained in step 8), and then the ultrasonic power was controlled at 200W for 15min to obtain the silicon ecological enzyme agricultural preparation. The slow-release silicon is wollastonite powder.

[0048] Comparative Example 11 An agricultural preparation Same as Example 1, except that step 9) is changed to: According to the material-liquid ratio of slow-release silicon to soluble silicon-enzyme hydrolysate of 1g:30mL, the slow-release silicon was added to the soluble silicon-enzyme hydrolysate obtained in step 8), mixed well, and then 0.1% genipin was added. The stirring speed was controlled at 80r / min, and the mixture was crosslinked at 50℃ for 50min to obtain the silicon ecological enzyme agricultural preparation. The slow-release silicon is wollastonite powder.

[0049] Effect verification I. Soil Improvement Performance Testing Using a compacted soil experimental field as the experimental object, the soil improvement performance of the agricultural agents prepared in Examples 1-3 and Comparative Examples 1-11 was tested (at a dosage of 100 mL of agent / acre, diluted with 30 liters of water, and then sprayed evenly on the soil surface). The results of the soil improvement performance test are shown in Table 2. Table 2 Results of Soil Improvement Performance Tests

[0050] As shown in Table 2, the silicon-based ecological enzyme agricultural preparation with dual functions of bioactivation and physical protection provided by this invention can effectively reduce soil salinity, increase organic matter content, soil pH, ammonium nitrogen content, available phosphorus content, and available potassium content, thus exhibiting good soil environment improvement effects.

[0051] II. Crop Performance Testing Rice and cantaloupe were used as test crops. The crop performance of the agricultural preparations prepared in Examples 1-3 and Comparative Examples 1-11 was tested (100 mL of preparation per mu was diluted with 50 liters of water and sprayed evenly on the soil surface). The results of the crop performance test are shown in Table 3. Table 3 Crop performance test results

[0052] As shown in Table 3, the silicon-based ecological enzyme agricultural preparation with dual functions of bioactivation and physical protection provided by this invention can effectively improve crop yield per acre and root health index, and reduce the incidence of pests and diseases, thus having a good effect on improving crop performance.

[0053] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit it. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that modifications or equivalent substitutions can still be made to the specific implementation of the present invention. Any modifications or equivalent substitutions that do not depart from the spirit and scope of the present invention should be covered within the scope of protection of the claims of the present invention.

Claims

1. A method for preparing a silicon-based ecological enzyme agricultural preparation with dual functions of bioactivation and physical protection, characterized in that, Includes the following steps: 1) Add the fermentation supplement to the organic waste to obtain the fermentation raw material, then add the compound microbial agent to the fermentation raw material and ferment in stages to obtain the fermentation liquid; 2) Add the compound enzyme preparation to the fermentation broth obtained in step 1), and perform enzymatic hydrolysis to obtain the hydrolysate; 3) Add silicon source to the enzymatic hydrolysate obtained in step 2) to obtain the silicon ecological enzyme agricultural preparation with dual functions of bioactivation and physical protection.

2. The method for preparing a silicon-based ecological enzyme agricultural preparation with dual functions of bioactivation and physical protection according to claim 1, characterized in that, In step 1), The fermentation supplement is composed of humic acid, seaweed extract, bentonite and chitin in a mass ratio of (4-6):(2-4):(0.5-1.5):(0.5-1.5); The method for preparing the organic waste includes the following steps: mixing agricultural waste and livestock manure, adjusting the C / N ratio to 30:1 to obtain a premix, and then subjecting the premix to physical-chemical synergistic treatment to obtain the organic waste.

3. The method for preparing a silicon-based ecological enzyme agricultural preparation with dual functions of bioactivation and physical protection according to claim 1, characterized in that, In step 1), the mass ratio of the fermentation supplement to the organic waste is 1:(30-40).

4. The method for preparing a silicon-based ecological enzyme agricultural preparation with dual functions of bioactivation and physical protection according to claim 1, characterized in that, In step 1), the compound microbial agent is composed of Bacillus subtilis, thermophilic methanococcus, Bacillus megaterium and azotobacter chrysogenum in a mass ratio of (5-7):(3-5):(2-4):(1-3).

5. The method for preparing a silicon-based ecological enzyme agricultural preparation with dual functions of bioactivation and physical protection according to claim 1, characterized in that, In step 1), the mass ratio of the fermentation raw material to the compound microbial agent is 50:(1-3).

6. The method for preparing a silicon-based ecological enzyme agricultural preparation with dual functions of bioactivation and physical protection according to claim 1, characterized in that, In step 1), the segmented fermentation specifically refers to: Aerobic start-up stage: In an environment with an oxygen content of 20%, control the stirring speed at 60 r / min and ferment at a constant temperature of 38-42℃ for 12-36 h; Anaerobic fermentation stage: ferment at a constant temperature of 45-48℃ for 4-6 days in an environment with oxygen content ≤1%. During this period, the stirring speed is controlled at 50r / min and stirred for 15min every 12h. Microaerobic ripening stage: Ferment at a constant temperature of 35-37℃ for 5-7 days in an environment with 5% oxygen content. During this period, the stirring speed is controlled at 30r / min and stirred for 5min every 24 hours.

7. The method for preparing a silicon-based ecological enzyme agricultural preparation with dual functions of bioactivation and physical protection according to claim 1, characterized in that, In step 2), the compound enzyme preparation is composed of cellulase, protease, hemicellulase and pectinase mixed in a mass ratio of (2-4):(2-4):(1-3):(0.5-1.5).

8. The method for preparing a silicon-based ecological enzyme agricultural preparation with dual functions of bioactivation and physical protection according to claim 1, characterized in that, In step 2), the ratio of the compound enzyme preparation to the fermentation broth is 1g:(20-30)mL.

9. The method for preparing a silicon-based ecological enzyme agricultural preparation with dual functions of bioactivation and physical protection according to claim 1, characterized in that, Step 3), the supplementary silicon source, specifically includes the following steps: 1) Add soluble silicon to the enzymatic hydrolysate, mix well, then add 0.3%wt EDTA-2Na, react to obtain soluble silicon-enzymatic hydrolysate; 2) Add the slow-release silicon to the soluble silicon-enzyme hydrolysate obtained in step 1), mix well, sonicate, add 0.1% genipin, crosslink, and obtain the silicon ecological enzyme agricultural preparation.

10. A silicon-based ecological enzyme agricultural preparation with dual functions of bioactivation and physical protection, prepared by a method according to any one of claims 1 to 9.

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