Constant-temperature rotary table type cell resuscitator

Abstract

The invention relates to the technical field of medical biological experiment instruments, and discloses a constant-temperature rotary table type cell resuscitation instrument which comprises an instrument body, a water bath is formed in one side of the instrument body, a heating tank is installed in the water bath, the top of the heating tank is rotationally connected with a first gear ring through a connecting ring, and the top of the first gear ring is fixedly connected with a circular plate. The top of the circular plate is rotatably connected with a plurality of containers, the outer walls of the containers are fixedly connected with first gears, the top of the circular plate is rotatably connected with a second gear ring, the inner wall of the second gear ring is in meshed connection with the outer walls of the first gears, the inner wall of the first gear ring is in meshed connection with a second gear, the top of the instrument body is fixedly connected with a rotating base and a mechanical arm, and a liquid nitrogen bin is formed in one side of the instrument body. The container is driven by the planetary gear transmission structure to do revolution and rotation composite motion, uniform heating is guaranteed, the mechanical arm is matched with the liquid nitrogen bin to achieve automatic grabbing and transferring, the operation risk is reduced, and the resuscitation efficiency is improved.

Description

Technical Field

[0001] This invention relates to the field of medical biological experimental equipment technology, and in particular to a constant temperature rotary benchtop cell resuscitation device. Background Technology

[0002] Cell resuscitation is a crucial step in biomedical research and clinical treatment. Its core purpose is to rapidly thaw cell samples preserved in liquid nitrogen or ultra-low temperature environments and restore their physiological activity. Most existing conventional cell resuscitation equipment uses a general-purpose constant temperature water bath as the basic heating medium. Experimenters need to manually remove the cryopreservation tubes and place them in a preheated water bath for heating.

[0003] While existing constant temperature water baths can heat water to provide a basic temperature environment through heating elements, most of them use static heating or simple unidirectional linear reciprocating oscillation in actual use. This single movement mode prevents the cell suspension inside the cryovial from generating sufficient convective mixing, making it difficult for the liquid inside the tube to achieve rapid temperature equilibrium between the inside and outside in a short time. Often, the liquid near the tube wall has already thawed while the sample in the center remains frozen. This uneven heating will cause ice crystal recrystallization during thawing, which can puncture the cell membrane, ultimately resulting in low cell recovery survival rate and impaired sample biological activity. This seriously affects the accuracy of subsequent experimental data and cannot meet the stringent requirements of high-precision biological experiments for sample consistency and recovery quality.

[0004] Therefore, this invention proposes a constant-temperature rotary tabletop cell resuscitation device to address the shortcomings of existing technologies. Summary of the Invention

[0005] In view of the fact that the existing constant temperature rotary benchtop cell resuscitation instruments have a relatively simple structure, often use static or single oscillation heating methods which result in uneven heating of samples, and that manual transfer of cryopreservation tubes is cumbersome and poses a risk of frostbite, this invention aims to provide a constant temperature rotary benchtop cell resuscitation instrument with an improved structure that can effectively solve the above problems.

[0006] The present invention provides a constant temperature rotary tabletop cell resuscitation device, comprising: a device body, and a water bath disposed inside one side of the device body; The water bath contains a heating tank. A connecting ring is fixedly connected to the top of the heating tank. A gear ring is rotatably connected to the top of the connecting ring. A circular plate is fixedly connected to the top of the gear ring. Multiple containers are rotatably connected to the top of the circular plate. Gear 1 is fixedly connected to the outer wall of the multiple containers. A gear ring 2 is rotatably connected to the top of the circular plate. The inner wall of gear ring 2 meshes with the outer wall of multiple gear 1. Gear 2 is meshed with the inner wall of gear ring 1. A circular shaft is fixedly connected to the top of gear 2. Furthermore, a rotating base is fixedly connected to one side of the top of the instrument body, and a robotic arm is fixedly connected to the top of the rotating base.

[0007] Preferably, each of the multiple containers has a gap groove inside, and a container bottle is fixedly connected to the inner wall of each of the multiple containers.

[0008] Preferably, springs are fixedly connected to both sides of the inner wall of the plurality of containers, and a flexible block is fixedly connected to one side of each of the plurality of springs.

[0009] Preferably, a fixing strip is rotatably connected to the rear side of the outer wall of the circular shaft, a cover plate is slidably connected to the top of the instrument body, and a handle is fixedly connected to the front side of the cover plate.

[0010] Preferably, a liquid nitrogen chamber is provided inside one side of the instrument body, and a cryopreservation tank is installed inside the liquid nitrogen chamber.

[0011] Preferably, a second cover plate is installed on the top of the liquid nitrogen tank, a buckle is fixedly connected to the rear side of the second cover plate, and a second handle is fixedly connected to the front side of the second cover plate.

[0012] Preferably, a smart screen is embedded in the top front side of the instrument body, and the smart screen is electrically connected to the internal control system of the instrument body.

[0013] Preferably, a knob is installed on the front of the instrument body, and the knob is used to control the emergency stop of the equipment.

[0014] Preferably, each of the four bottom corners of the instrument body is fixedly connected to a support column, and the bottom of each of the support columns is fixedly connected to an anti-slip pad.

[0015] Preferably, a liquid level sensor is fixedly connected to one corner of the inside of the water bath, and a drain valve is fixedly connected to one side of the instrument body, the drain valve being connected to the inner wall of the water bath.

[0016] Compared with the prior art, the beneficial effects of the present invention are: 1. This invention solves the problem of uneven sample heating caused by relying solely on a single oscillation or static water bath for cell resuscitation in the prior art by setting up a planetary gear transmission structure consisting of a heating tank, a gear ring, and a circular plate. This allows the cryopreservation tube to simultaneously undergo a combined revolution and rotation motion in a constant temperature water bath, ensuring rapid and uniform heating and resuscitation of the cell suspension, and significantly improving cell survival rate.

[0017] 2. This invention solves the problem of cumbersome manual transfer of cryopreservation tubes in the prior art, which is prone to causing frostbite to operators, by integrating a liquid nitrogen chamber on one side of the instrument and using a rotating base in conjunction with a robotic arm for automated grasping and transfer. It realizes fully automatic contactless transfer from the liquid nitrogen storage environment to the water bath heating environment, reduces the risk of biological sample contamination and ensures the safety of experimental personnel.

[0018] 3. When the cryopreservation tube is inserted into the container bottle, the flexible block presses against the outer wall of the cryopreservation tube under the action of the spring force. The gap groove provides a buffer space for the flexible block to retract under pressure, thereby realizing flexible adaptive clamping of cryopreservation tubes of different sizes, ensuring that the cryopreservation tube is stable in position and not damaged by hard impact during the double rotation and shaking process.

[0019] 4. The present invention has a handle fixedly connected to the front side of the plate. The handle drives the cover plate to slide on the top of the instrument body to open and close the water bath, thereby reducing heat loss and maintaining a constant internal temperature environment during heating. Attached Figure Description

[0020] Figure 1 This is a perspective view of a constant-temperature rotary benchtop cell resuscitation device proposed in this invention; Figure 2 This is a front view of a constant-temperature rotary benchtop cell resuscitation device proposed in this invention; Figure 3 This is a cross-sectional view of the interior of a constant-temperature rotary benchtop cell resuscitation device proposed in this invention. Figure 4 This is a schematic diagram of the mechanical transport in a constant-temperature rotary tabletop cell resuscitation device proposed in this invention; Figure 5 This is a schematic diagram of the internal shaking mechanism in a constant-temperature rotary tabletop cell resuscitation device proposed in this invention. Figure 6 This is a cross-sectional view of the flexible pressing action in a constant-temperature rotary tabletop cell resuscitation device proposed in this invention.

[0021] in: 1. Instrument body; 2. Water bath; 3. Heating tank; 4. Connecting ring; 5. Gear ring one; 6. Circular plate; 7. Container; 8. Gap groove; 9. Container bottle; 10. Spring; 11. Flexible block; 12. Gear one; 13. Gear ring two; 14. Gear two; 15. Circular shaft; 16. Fixing strip; 17. Cover plate one; 18. Handle one; 19. Rotating base; 20. Robotic arm; 21. Liquid nitrogen chamber; 22. Cryopreservation tube tank; 23. Cover plate two; 24. Buckle; 25. Handle two; 26. Smart screen; 27. Knob; 28. Support column; 29. ​​Anti-slip mat; 30. Liquid level sensor; 31. Drain valve. Detailed Implementation

[0022] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0023] Example: Please refer to Figures 1 to 6 This invention provides a constant-temperature rotary benchtop cell resuscitation device, which aims to solve the structural defects in the prior art, such as uneven heating and low efficiency in the cell resuscitation process, as well as the cumbersome operation and risk of frostbite associated with manual transfer of cryopreservation tubes.

[0024] Please refer to Figure 1 , Figure 2 and Figure 3A constant-temperature rotary benchtop cell resuscitation device includes a body 1 and a water bath 2 located inside one side of the body 1. The body 1 serves as the mounting base and outer shell structure of the entire device. The water bath 2 is used to contain heating media to create a constant-temperature resuscitation environment. A heating tank 3 is installed inside the water bath 2 to heat the water inside the water bath 2 and maintain a constant temperature. A connecting ring 4 is fixedly connected to the top of the heating tank 3, serving as a supporting intermediate component. A gear ring 5 is rotatably connected to the top of the connecting ring 4, allowing the gear ring 5 to rotate circumferentially relative to the connecting ring 4. A circular plate 6 is fixedly connected to the top of the gear ring 5, serving as a support platform that rotates synchronously with the gear ring 5. Multiple sets of containers 7 are rotatably connected to the top circumference of the circular plate 6, used to place cryopreservation tubes to be resuscitated and to support the circular plate 6. The plate 6 rotates independently. Gear 12 is fixedly connected to the outer wall of multiple sets of containers 7. Gear ring 2 13 is rotatably connected to the top of the circular plate 6. The inner wall of gear ring 2 13 is meshed with the outer wall of multiple sets of gear 12. The rotation of containers 7 is driven by the meshing transmission between gear ring 2 13 and gear 12. Gear 2 14 is meshed with the inner wall of gear ring 5. Gear 2 14 serves as the power input end. A circular shaft 15 is fixedly connected to the top of gear 2 14. The circular shaft 15 is connected to an external drive motor. When the circular shaft 15 drives gear 2 14 to rotate, gear 2 14 drives gear ring 15 and the circular plate 6 to rotate as a whole, so that containers 7 revolve around the center. At the same time, due to the meshing of gear ring 2 13, gear 12 and containers 7 are forced to rotate, thus realizing a compound rocking motion of revolution and rotation.

[0025] Please refer to Figure 1 and Figure 4 A rotating base 19 is fixedly connected to one side of the top of the instrument body 1. The rotating base 19 is used to provide horizontal rotational freedom. A robotic arm 20 is fixedly connected to the top of the rotating base 19. The robotic arm 20 serves as an automated actuator. The robotic arm 20 has multi-degree-of-freedom adjustment capabilities and can extend to different areas of the instrument body 1 to perform operations. Specifically, it is used to grab cryopreservation tubes and automatically transfer them between the liquid nitrogen storage area and the water bath heating area. Together with the aforementioned rotating water bath structure, it constitutes a fully automated cell resuscitation processing system.

[0026] Please refer to Figure 3 and Figure 6The container 7 has a gap groove 8 inside, and a container bottle 9 is fixedly connected to the inner wall of the container 7. Springs 10 are fixedly connected to both sides of the inner wall of the container 7. A flexible block 11 is fixedly connected to one side of the spring 10. The flexible block 11 is located in the internal space of the container bottle 9. When the cryopreservation tube is inserted into the container bottle 9, the flexible block 11 presses against the outer wall of the cryopreservation tube under the elastic force of the spring 10. The gap groove 8 provides a buffer space for the flexible block 11 to move back under pressure, thereby realizing flexible adaptive clamping of cryopreservation tubes of different sizes, ensuring that the cryopreservation tube is stable and not damaged by hard impact during double rotation and shaking.

[0027] Please refer to Figure 2 and Figure 3 A fixing strip 16 is rotatably connected to the rear side of the outer wall of the circular shaft 15. The fixing strip 16 is used to assist in supporting the rotational stability of the circular shaft 15. A cover plate 17 is slidably connected to the top of the instrument body 1. The position of the cover plate 17 corresponds to the top opening of the water bath 2. A handle 18 is fixedly connected to the front side of the cover plate 17. The handle 18 drives the cover plate 17 to slide on the top of the instrument body 1, realizing the opening and closing of the water bath 2, reducing heat loss and maintaining a constant internal temperature environment during heating operation.

[0028] Please refer to Figure 1 and Figure 4 The instrument body 1 has a liquid nitrogen chamber 21 inside one side. The liquid nitrogen chamber 21 is equipped with a cryopreservation tube tank 22, which is used to store samples to be resuscitated in an orderly manner. The liquid nitrogen chamber 21 is equipped with a cover plate 23. The rear side of the cover plate 23 is fixedly connected with a buckle 24, and the front side of the cover plate 23 is fixedly connected with a handle 25. The cover plate 23 is fixedly locked to the top of the instrument body 1 by the buckle 24, which together with the cover plate 23, seals the liquid nitrogen chamber 21, prevents excessive evaporation of the liquid nitrogen inside, and provides a front storage station for the robotic arm 20 to grasp and operate.

[0029] As a preferred embodiment, please refer to Figure 1 and Figure 2 The instrument body 1 has a smart screen 26 embedded on the top front side. The smart screen 26 is electrically connected to the internal control circuit of the instrument body 1. The smart screen 26 is used to display the real-time temperature and motor speed inside the water bath 2 and provide a touch operation interface for users to set heating and shaking parameters. A knob 27 is installed on the front of the instrument body 1. The knob 27 is used as a physical control switch to cut off the power supply in an emergency to stop the equipment in an emergency and ensure the safety of the experimental operation process.

[0030] As another preferred embodiment, please refer to Figure 2Support columns 28 are fixedly connected to the four corners of the bottom of the instrument body 1. The support columns 28 are used to raise the bottom of the instrument body 1 to facilitate heat dissipation and drainage. Anti-slip pads 29 are fixedly connected to the bottom of the support columns 28. The anti-slip pads 29 are made of high friction coefficient silicone material. The anti-slip pads 29 are used to increase the friction between the instrument body 1 and the table surface to prevent the vibration of the motor and gear transmission from causing the equipment to shift or slip.

[0031] As another preferred embodiment, please refer to Figure 3 A liquid level sensor 30 is fixedly connected to one corner inside the water bath 2. The probe of the liquid level sensor 30 is lower than the top of the heating tank 3. The liquid level sensor 30 is used to monitor the water level in the water bath 2 in real time and send a signal to the control system to trigger an alarm or power off when the water level is too low, so as to prevent the heating tank 3 from being damaged due to lack of water and dry burning. A drain valve 31 is fixedly connected to one side of the instrument body 1. The water inlet of the drain valve 31 passes through the side wall of the instrument body 1 and is connected to the bottom of the water bath 2. The drain valve 31 is used to quickly discharge the waste liquid in the water bath 2 after the resuscitation task is completed.

[0032] Working principle: First, an appropriate amount of medium is injected into the water bath 2 and the constant temperature parameters are set through the smart screen 26. The heating tank 3 is powered on to maintain the water bath environment temperature. The operator opens the cover plate 23 and drives the rotating base 19 and the robotic arm 20 to move through the control system. The robotic arm 20 extends into the cryopreservation tube slot 22 inside the liquid nitrogen chamber 21 to grab the cryopreservation tube. Then the robotic arm 20 moves the cryopreservation tube above the water bath 2 and puts it into the container 7. During this process, the spring 10 releases its elastic force to push the flexible block 11 against the outer wall of the cryopreservation tube. The flexible contact characteristics of the flexible block 11 and the constant pressure of the spring 10 ensure that the cryopreservation tube is firmly fixed in the container 9.

[0033] After fixing, cover plate 17 is placed on top, and the external motor is started to drive the circular shaft 15 to rotate. The circular shaft 15 drives the top fixed connecting gear 14 to rotate. Gear 14 drives gear ring 5, circular plate 6, and gear ring 13 to rotate. Gear ring 5 drives circular plate 6, along with multiple sets of containers 7, to revolve around the center. At the same time, the rotation of gear ring 13 drives gear 12, which meshes with it, to rotate in place, forcing the containers 7 to rotate around their own axis. This achieves a combined shaking of revolution and rotation of containers 7 in the hot water of the water bath 2, so that the cell suspension in the cryopreservation tube is rapidly and evenly heated and revived.

[0034] If the liquid level in the water bath 2 is lower than the safety line, the liquid level sensor 30 senses and sends a signal to cut off the power to the heating tank 3 to prevent dry burning. After the recovery is completed, the drain valve 31 is opened to drain the liquid. In an emergency, the knob 27 is rotated to force the equipment to stop running.

[0035] In the description of this invention, the terms "upper," "lower," "front," "rear," "left," "right," "top," "bottom," "vertical," and "horizontal," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only to describe the invention and not to require the invention to be constructed or operated in a specific orientation; therefore, they should not be construed as limitations on the invention. The terms "connected" and "linked" in this invention should be interpreted broadly. For example, they can refer to a connection or a detachable connection; they can refer to a direct connection or an indirect connection through intermediate components. Those skilled in the art can understand the specific meaning of the above terms based on the specific circumstances.

[0036] The above description represents the preferred mode of operation of the present invention. The specific operational modes are provided solely for a better understanding of the invention's concept. Those skilled in the art can make various improvements or equivalent substitutions based on the principles of this invention, and these improvements or equivalent substitutions are also considered to fall within the scope of protection of this invention.

Claims

1. A constant temperature rotary tabletop cell resuscitation instrument, comprising an instrument body (1), wherein a water bath (2) is provided inside one side of the instrument body (1). Its features are, The water bath (2) is equipped with a heating tank (3). A connecting ring (4) is fixedly connected to the top of the heating tank (3). A gear ring (5) is rotatably connected to the top of the connecting ring (4). A circular plate (6) is fixedly connected to the top of the gear ring (5). Multiple containers (7) are rotatably connected to the top of the circular plate (6). Gears (12) are fixedly connected to the outer walls of the multiple containers (7). A gear ring (13) is rotatably connected to the top of the circular plate (6). The inner wall of the gear ring (13) meshes with the outer wall of the multiple gears (12). Gears (14) are meshed with the inner wall of the gear ring (5). A circular shaft (15) is fixedly connected to the top of the gear ring (1). A rotating base (19) is fixedly connected to one side of the top of the instrument body (1). A robotic arm (20) is fixedly connected to the top of the rotating base (19).

2. The isothermal rotary tabletop cell resuscitation device according to claim 1, characterized in that, Each of the multiple containers (7) has a gap groove (8) inside, and each of the multiple containers (7) has a container bottle (9) fixedly connected to its inner wall.

3. The isothermal rotary tabletop cell resuscitation device according to claim 1, characterized in that, Springs (10) are fixedly connected to both sides of the inner wall of the multiple containers (7), and flexible blocks (11) are fixedly connected to one side of each of the multiple springs (10).

4. The isothermal rotary tabletop cell resuscitation device according to claim 1, characterized in that, The outer wall of the circular shaft (15) is rotatably connected to a fixing strip (16), and the top of the instrument body (1) is slidably connected to a cover plate (17), and the front side of the cover plate (17) is fixedly connected to a handle (18).

5. The isothermal rotary tabletop cell resuscitation device according to claim 1, characterized in that, The instrument body (1) has a liquid nitrogen tank (21) inside one side, and a cryopreservation tank (22) is installed inside the liquid nitrogen tank (21).

6. The isothermal rotary tabletop cell resuscitation device according to claim 5, characterized in that, The top of the liquid nitrogen tank (21) is equipped with a cover plate two (23), the rear side of the cover plate two (23) is fixedly connected with a buckle (24), and the front side of the cover plate two (23) is fixedly connected with a handle two (25).

7. The isothermal rotary tabletop cell resuscitation device according to claim 1, characterized in that, The instrument body (1) has a smart screen (26) embedded on the top front side, and the smart screen (26) is electrically connected to the internal control system of the instrument body (1).

8. The isothermal rotary tabletop cell resuscitation device according to claim 1, characterized in that, A knob (27) is installed on the front of the instrument body (1), and the knob (27) is used to control the emergency stop of the equipment.

9. A constant-temperature rotary benchtop cell resuscitation device according to claim 1, characterized in that, The bottom four corners of the instrument body (1) are all fixedly connected with support columns (28), and the bottom of the multiple support columns (28) are all fixedly connected with anti-slip pads (29).

10. A constant-temperature rotary benchtop cell resuscitation device according to claim 1, characterized in that, A liquid level sensor (30) is fixedly connected to one corner inside the water bath (2), and a drain valve (31) is fixedly connected to one side of the instrument body (1). The drain valve (31) is connected to the inner wall of the water bath (2).

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