Ultrasonic stimulation growth device for poria cocos strain

Abstract

The invention relates to the technical field of poria cocos strain planting, and discloses a poria cocos strain ultrasonic stimulation growth device which comprises a culture box and a control assembly, the culture box is provided with a layered culture frame, an environment regulation and control assembly and an ultrasonic stimulation assembly, and the ultrasonic stimulation assembly comprises a conduction plate net and a conduction surrounding net; the control assembly is used for cooperatively regulating and controlling the temperature and humidity parameters, the oxygen concentration parameters and the illumination parameters of the environment regulation and control assembly and the ultrasonic frequency parameters and the power parameters of the ultrasonic stimulation assembly according to different growth stages of the poria cocos strains. According to the invention, the cylindrical net structure is arranged in the culture dish to realize ultrasonic uniform coverage, so that the strain stimulated consistency is guaranteed; the butt joint design of the two conduction plates ensures the effectiveness of ultrasonic conduction and the precise effect of assisting stimulation; the layered layout and the vibration isolation base are matched with multiple culture dishes for synchronous culture, so that mutual interference is reduced; and the growth rule of the adaptive strain is regulated and controlled cooperatively by multiple parameters, so that the cultivation pertinence and efficiency are improved.

Description

Technical Field

[0001] This invention relates to the field of Poria cocos strain cultivation technology, specifically to a Poria cocos strain ultrasonic stimulation growth device. Background Technology

[0002] The proliferation efficiency of Poria cocos strains directly affects the yield and quality of subsequent Poria cocos cultivation. Traditional Poria cocos strain cultivation often uses static incubators with constant temperature and humidity, which results in slow strain growth, uneven mycelial viability, and weak stress resistance. In existing technologies, some studies have attempted to use ultrasound-assisted microbial cultivation.

[0003] Existing technology discloses an ultrasonic stimulation growth device for Poria cocos spores (application number: CN202422348749.7), specifically relating to the field of Poria cocos spore growth. The device includes an incubator with a water collection trough on the bottom inner wall and a water tank on one side of the top. One end of the water tank is connected to a water pipe, and a first water pump is installed in the middle of the water pipe. A return port is located on one side of the water collection trough, and a return conduit is connected to one side of the return port. The end of the return conduit away from the return port is connected to the water tank. A second water pump is installed on one side of the filter box. The first water pump transmits water from the water tank to a spray head via the water pipe, irrigating and cultivating the Poria cocos spores on the placement plate. Excess water drips down into the water collection trough through a through-hole. Activating the second water pump allows the water in the water collection trough to be pumped back into the water tank via the return conduit, thus achieving water recycling and saving water resources.

[0004] However, existing technologies, especially this particular solution, still have the following problems: The existing technology does not have a dedicated structure for uniform ultrasonic coverage and relies only on conventional ultrasonic application methods, which cannot achieve all-round enveloping stimulation of the bottom and inner wall of the culture dish. This results in uneven stimulation intensity of the Poria cocos strain and poor growth consistency of the strain in different areas, thus restricting the proliferation effect. The cultivation program lacks a targeted approach and cannot dynamically adapt to the growth patterns of the fungus, resulting in difficulty in improving the proliferation efficiency of the fungus. It also fails to design a synergistic regulation mechanism for environmental parameters (temperature, humidity, oxygen concentration, light) and ultrasonic parameters (frequency, power) to meet the physiological needs of different growth stages of the Poria cocos fungus. Summary of the Invention

[0005] The purpose of this invention is to provide a technical solution that achieves uniform ultrasonic coverage, ensures consistent stimulation of bacterial strains, and enables multi-parameter synergistic regulation to adapt to the growth patterns of bacterial strains, thereby improving the targeting and efficiency of cultivation and solving the problems in the prior art mentioned in the background.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: An ultrasonic stimulation growth device for Poria cocos strains, comprising: An incubator and control components, wherein the incubator is equipped with a layered culture rack, an environmental control component and an ultrasonic stimulation component, and the ultrasonic stimulation component includes a conductive plate mesh and a conductive enclosure mesh; The layered culture rack is provided with several placement bases, and each placement base is provided with a corresponding vibration isolation base. A culture dish is placed on the vibration isolation base. A conductive plate mesh is set at the bottom of the culture dish, and a conductive enclosure mesh is set on the inner wall. The conductive plate mesh and the conductive enclosure mesh are combined to form a cylindrical mesh structure, which is used to uniformly stimulate growth inside the culture dish with ultrasound. The culture dish is provided with an ultrasonic transmission plate one on the outside, and an ultrasonic transmission plate two is provided on the placement base; when the culture dish is placed on the vibration isolation base, the ultrasonic transmission plate one and the ultrasonic transmission plate two are in a docking state and can transmit ultrasonic waves. The control component is used to coordinate and regulate the temperature and humidity parameters, oxygen concentration parameters, and light parameters of the environmental control component, as well as the ultrasonic frequency and power parameters of the ultrasonic stimulation component, according to different growth stages of the Poria cocos strain.

[0007] Preferably, the ultrasonic stimulation component includes an ultrasonic generator and an ultrasonic transducer. The ultrasonic generator and the ultrasonic transducer are electrically connected. The ultrasonic transducer and the second ultrasonic transmission plate are connected by a coupling agent to realize the transmission of ultrasonic waves. The first ultrasonic transmission plate and the second ultrasonic transmission plate are detachably connected and a coupling agent is provided between them.

[0008] Preferably, a mesh conduction connector is installed through the side of the culture dish, which is used to connect the ultrasonic conduction plate and the conduction mesh and conduction enclosure mesh.

[0009] Preferably, the conductive plate mesh and the conductive enclosure mesh are integrally formed, and the mesh conductive connector is fixedly connected to the cylindrical mesh structure enclosed by the conductive plate mesh and the conductive enclosure mesh.

[0010] Preferably, the ultrasonic generators are configured in multiple groups, and the layered culture rack is configured as a multi-layer structure. The multiple groups of ultrasonic generators are respectively connected to different layers of the layered culture rack to apply differentiated ultrasonic frequencies and power stimulation to different layers of the layered culture rack.

[0011] Preferably, the culture dish is provided with a conductive frame inside, which is used for installing the conductive plate mesh and the conductive enclosure mesh, and the vibration isolation base is provided with a perforation hole.

[0012] Preferably, the inner wall of the culture dish is provided with a support protrusion for supporting the conductive frame, which is detachably installed inside the culture dish.

[0013] Preferably, the bottom of the incubator is provided with a mesh bottom plate for placing the layered culture rack, and the environmental control components include a temperature control module, a humidity control module, an oxygen control module, and a light control module.

[0014] Preferably, the incubator is equipped with a power supply component and a monitoring component. The power supply component includes a solar panel and an external power supply module, and the monitoring component includes a temperature sensor, a humidity sensor, an oxygen concentration sensor, a light sensor, and a camera module. The control component is used to control the environmental control component, the ultrasonic stimulation component, the power supply component, and the monitoring component.

[0015] Preferably, the control component includes a controller, a touch screen, and multiple sensors. The controller is electrically connected to the ultrasonic stimulation component and the environmental control component, respectively. The temperature sensor, humidity sensor, oxygen concentration sensor, and light sensor are embedded inside the incubator body for real-time acquisition of environmental parameters inside the incubator.

[0016] Technical effects and advantages of the present invention: The ultrasonic stimulation growth device for Poria cocos strain proposed in this invention has the following advantages compared with the prior art: This invention uses a control component as its core to coordinate the scheduling of environmental regulation and ultrasonic stimulation components; a cylindrical mesh structure enclosed by a conductive plate mesh and a conductive enclosure mesh forms a full-range ultrasonic coverage of the bottom and inner wall of the culture dish, and ultrasonic transmission is achieved by connecting the culture dish with two conductive plates on the mounting base; combined with the layout of the layered culture rack and vibration isolation base, it can adapt to the different growth stages of Poria cocos strains when adjusting parameters such as temperature, humidity, oxygen concentration, light, ultrasonic frequency, and power. The cylindrical mesh structure ensures uniform ultrasonic coverage, guaranteeing consistent stimulation of the microbial strains; the docking design of the two conductive plates ensures effective ultrasonic transmission, facilitating precise stimulation; the layered layout and vibration-isolated base allow for simultaneous cultivation in multiple culture dishes, reducing mutual interference; and the multi-parameter synergistic regulation adapts to the growth patterns of the microbial strains, improving the targeted nature and efficiency of cultivation. Attached Figure Description

[0017] Figure 1 This is one of the three-dimensional structural schematic diagrams of the ultrasonic stimulation growth device for Poria cocos strains of the present invention; Figure 2 This is the second three-dimensional structural schematic diagram of the ultrasonic stimulation growth device for Poria cocos strains of the present invention. Figure 3 This is a schematic diagram of the internal structure of the ultrasonic stimulation growth device for Poria cocos strains of the present invention. Figure 4 This is a schematic diagram of the layered culture rack and culture dish in an embodiment of the present invention; Figure 5 For the present invention Figure 4Enlarged structural diagram at point A; Figure 6 This is a schematic diagram of the vibration isolation base and culture dish in an embodiment of the present invention.

[0018] In the picture: 11. Incubator; 12. Ultrasonic generator; 13. Ultrasonic transducer; 14. Mesh base plate; 15. Environmental control components; 16. Control components; 21. Layered culture rack; 22. Placement base; 23. Culture dish; 24. Vibration isolation base; 25. Ultrasonic transmission plate one; 26. Ultrasonic transmission plate two; 27. Support protrusion; 28. Transmission frame; 29. ​​Transmission plate mesh; 210. Transmission enclosure mesh; 211. Mesh transmission connector; 212. Leakage hole. Detailed Implementation

[0019] The subject matter described herein will now be discussed with reference to exemplary embodiments. It should be understood that these embodiments are discussed only to enable those skilled in the art to better understand and implement the subject matter described herein, and changes may be made to the function and arrangement of the elements discussed without departing from the scope of this specification. Various processes or components may be omitted, substituted, or added as needed in the examples. Furthermore, features described in some examples may be combined in other examples.

[0020] The invention provides, for example Figures 1 to 6 As shown, a device for ultrasonic stimulation and growth of Poria cocos strain includes: The incubator 11 and control component 16 are provided. The incubator 11 is equipped with a layered culture rack 21, an environmental control component 15 and an ultrasonic stimulation component. The ultrasonic stimulation component includes a conductive plate mesh 29 and a conductive enclosure mesh 210. The layered culture rack 21 is provided with several placement bases 22, and each placement base 22 is provided with a corresponding vibration isolation base 24. A culture dish 23 is placed on the vibration isolation base 24. A conductive plate mesh 29 is set at the bottom of the culture dish 23, and a conductive enclosure mesh 210 is set on the inner wall. The conductive plate mesh 29 and the conductive enclosure mesh 210 are enclosed to form a cylindrical mesh structure, which is used to uniformly stimulate growth inside the culture dish 23 with ultrasound. The culture dish 23 is provided with an ultrasonic transmission plate 25 on its exterior, and an ultrasonic transmission plate 26 is provided on the placement base 22. When the culture dish 23 is placed on the vibration isolation base 24, the ultrasonic transmission plate 25 and the ultrasonic transmission plate 26 are in a docking state and can transmit ultrasonic waves. The control component 16 is used to coordinate and regulate the temperature and humidity parameters, oxygen concentration parameters, and light parameters of the environmental control component 15 with the ultrasonic frequency and power parameters of the ultrasonic stimulation component according to the different growth stages of the Poria cocos strain.

[0021] Working principle: With the control component 16 as the core, it coordinates the environmental regulation and ultrasonic stimulation components; through the cylindrical mesh structure enclosed by the conduction plate mesh 29 and the conduction enclosure mesh 210, it forms an all-round ultrasonic coverage on the bottom and inner wall of the culture dish 23, and the ultrasonic wave is transmitted by the docking of the culture dish 23 and the two conduction plates on the placement base 22; combined with the layout of the layered culture rack 21 and the vibration isolation base 24, it can adapt to the needs of different growth stages of Poria cocos strains when adjusting parameters such as temperature, humidity, oxygen concentration, light, ultrasonic frequency, and power. The cylindrical mesh structure ensures uniform ultrasonic coverage, guaranteeing consistent stimulation of the strains; the docking design of the two conduction plates ensures effective ultrasonic transmission, facilitating precise stimulation; the layered layout and vibration isolation base 24 allow for simultaneous cultivation in multiple culture dishes 23, reducing mutual interference; and multi-parameter synergistic regulation adapts to the growth patterns of the strains, improving the targeted nature and efficiency of cultivation.

[0022] like Figure 2 As shown, considering the energy loss that easily occurs during ultrasonic wave transmission, and the fact that the ease of disassembly and maintenance of the equipment directly affects its efficiency, and to ensure a tight fit at the transmission interface to improve the ultrasonic transmission effect, an ultrasonic generator 12 and a transducer are added to the ultrasonic stimulation component. A coupling agent is used to achieve efficient connection between the transducer and the transmission plate, and between the transmission plates themselves. A detachable docking structure is also designed. The ultrasonic stimulation component includes an ultrasonic generator 12 and an ultrasonic transducer 13. The ultrasonic generator 12 and the ultrasonic transducer 13 are electrically connected. The ultrasonic transducer 13 is connected to the ultrasonic transmission plate 26 via a coupling agent to achieve ultrasonic wave transmission. The ultrasonic transmission plate 25 and the ultrasonic transmission plate 26 are detachably docked, and a coupling agent is placed between them.

[0023] like Figure 5 and Figure 6 As shown, gaps between the ultrasonic transducer plate and the cylindrical mesh structure can lead to uneven vibration transmission, affecting the uniformity of stimulation. To construct a complete and stable ultrasonic transmission path, a mesh transmission connector 211 is provided on the side of the culture dish 23. This connector is specifically used to connect the ultrasonic transducer plate 25 with the transducer mesh 29 and the transmission mesh 210, ensuring uninterrupted energy transmission. The mesh transmission connector 211 is installed through the side of the culture dish 23, and it is used to connect the ultrasonic transducer plate 25 with the transducer mesh 29 and the transmission mesh 210.

[0024] like Figure 6As shown, to avoid assembly deviations when the conductive plate mesh 29 and the conductive enclosure mesh 210 are installed separately, to ensure the regularity of the cylindrical ultrasonic field formed by the two, and to prevent stimulation imbalance caused by loosening at the connection during vibration, the conductive plate mesh 29 and the conductive enclosure mesh 210 are designed as an integral molded structure, and a fixed connection method is adopted to enhance the connection stability between the mesh conductive connector 211 and the cylindrical mesh structure. The conductive plate mesh 29 and the conductive enclosure mesh 210 are set as an integral molded structure, and the mesh conductive connector 211 is fixedly connected to the cylindrical mesh structure enclosed by the conductive plate mesh 29 and the conductive enclosure mesh 210.

[0025] Given that the bacterial strains at different levels of the tiered culture rack 21 may be at different growth stages, a single ultrasound parameter is insufficient to meet the cultivation needs of all levels. To achieve targeted stimulation and improve overall proliferation efficiency, multiple sets of ultrasound generators 12 are configured, each corresponding to a different level of the tiered culture rack 21, and their frequency and power can be independently adjusted. Multiple sets of ultrasound generators 12 are configured, and the tiered culture rack 21 is set as a multi-layered structure. The multiple sets of ultrasound generators 12 are respectively connected to different levels of the tiered culture rack 21 to apply differentiated ultrasound frequencies and power stimulation to different layers of the tiered culture rack 21.

[0026] To ensure stable installation of the conductive plate mesh 29 and conductive enclosure mesh 210, and to guarantee uniform circulation of temperature, humidity, and oxygen within the incubator 11, preventing local environmental imbalances from affecting bacterial growth and to prevent vibration interference between adjacent culture dishes 23 that could lead to inconsistent stimulation effects, a conductive frame 28 is installed inside the culture dish 23. A perforation 212 is provided in the vibration isolation base 24, which is made of an elastic damping material. The conductive frame 28 is installed inside the culture dish 23 for installing the conductive plate mesh 29 and conductive enclosure mesh 210. The perforation 212 in the vibration isolation base 24 ensures the circulation of temperature, humidity, and oxygen within the incubator 11. The vibration isolation base 24 is made of an elastic damping material, such as silicone or nitrile rubber, to block the transmission of ultrasonic vibrations between different culture dishes 23, achieving independent and interference-free ultrasonic stimulation for each culture dish 23.

[0027] Specifically, the inner wall of the culture dish 23 is provided with a support protrusion 27, which is used to support the conduction frame 28, and the conduction frame 28 is detachably installed inside the culture dish 23.

[0028] To provide precise and controllable environmental conditions for the growth of Poria cocos strains, and to address the issues of narrow parameter range and low precision in traditional control equipment, while ensuring the stable placement of the tiered culture rack 21 and the circulation of the environment within the chamber, a perforated bottom plate 14 is installed at the bottom of the incubator 11. The environmental control components 15 are further subdivided into four modules: temperature, humidity, oxygen, and light, with each module's core components and control range clearly defined. The bottom of the incubator 11 is equipped with a perforated bottom plate 14 for placing the tiered culture rack 21. The environmental control components 15 include a temperature control module, a humidity control module, an oxygen control module, and a light control module. The temperature control module consists of a heating element and a semiconductor cooler, which are respectively installed on the inner wall of the incubator 11. Together with the temperature sensor, they can accurately control the temperature inside the incubator within the range of 22℃-28℃. The humidity control module includes a humidifier and a dehumidifier, which together with the humidity sensor can control the relative humidity inside the incubator within the range of 60%-85%. The oxygen control module includes a miniature air pump and a gas flow meter. The air pump delivers sterile oxygen into the incubator through a pipeline, and the flow meter is used to control the oxygen input rate to ensure the oxygen requirements for bacterial growth.

[0029] To prevent the cultivation process from terminating due to an interruption of external power supply, and to achieve comprehensive monitoring of environmental parameters and equipment operating status within the chamber, allowing for timely detection and adjustment of abnormalities, and providing a continuous and stable guarantee for the growth of the microorganisms, the incubator 11 is equipped with a power supply component consisting of a solar panel and an external power supply module, as well as a monitoring component including various sensors and cameras, all uniformly controlled by the control component 16. The incubator 11 is equipped with a power supply component and a monitoring component. The power supply component includes a solar panel and an external power supply module, while the monitoring component includes a temperature sensor, a humidity sensor, an oxygen concentration sensor, a light sensor, and a camera module. The control component 16 is used to control the environmental control component 15, the ultrasonic stimulation component, the power supply component, and the monitoring component.

[0030] To simplify equipment operation procedures, reduce parameter errors caused by manual intervention, and simultaneously achieve automatic matching of the strain growth stage with ultrasonic and environmental parameters, thereby improving the accuracy and repeatability of cultivation, the control component 16 is designed as a combination of a controller, a touch screen, and multiple sensors. It has a built-in parameter database and supports parameter input, real-time display, and automatic adaptation functions. The control component 16 includes a controller, a touch screen, and multiple sensors. The controller is electrically connected to the ultrasonic stimulation component and the environmental control component 15, respectively. The temperature sensor, humidity sensor, oxygen concentration sensor, and light sensor are embedded inside the incubator 11 to collect environmental parameters in real time. The touch screen is located on the outside of the incubator 11 and is used to input cultivation parameters (ultrasonic frequency, power, stimulation duration, temperature and humidity range, etc.) and display the environmental data and equipment operating status in real time. The controller has a built-in parameter database for the growth stages of the Poria cocos strain and can automatically match ultrasonic stimulation schemes and environmental parameters for different growth cycles.

[0031] It should be noted that, combining the growth characteristics of Poria cocos strains (adaptation period, rapid mycelial growth period, sclerotium formation period), environmental parameter requirements, and ultrasonic stimulation patterns, a collaborative control logic of "staged precise matching + sensor feedback closed loop" was designed to completely solve the defect of "poor coordination" in existing technologies. The specific logic is as follows: I. Core Prerequisite for Collaborative Control: Delineating the Key Growth Stages of Poria Cocos Mycelium; Based on the "growth stage timing module" built into the control component 16, and combined with the mycelial morphology sensor (newly added, used to determine the growth status in real time), the cultivation process is divided into 3 core stages, each corresponding to a unique combination of "environmental parameters + ultrasonic parameters": 1. Adaptation period (0-48h): The strain is activated from a dormant state, its metabolism is slow, and it requires a mild environment and low-intensity ultrasound stimulation; 2. Rapid mycelial growth period (48-168h): The mycelium proliferates vigorously and requires sufficient nutrition, suitable temperature and humidity, and moderate ultrasonic stimulation to promote increased cell membrane permeability. 3. Sclerotium formation period (168-360h): Mycelia aggregate to form sclerotia. The metabolic intensity decreases, and a stable environment and low-intensity ultrasound stimulation are required to avoid damage to the sclerotia.

[0032] II. Phased collaborative control logic (precise matching of environmental parameters and ultrasound parameters); (a) Adaptation period (0-48h): Gentle activation to shorten the adaptation cycle. 1. Environmental control component 15 parameter settings: -Temperature: 25-26℃ (close to the optimal initiation temperature of mycelium, avoiding low temperature inhibition or high temperature stress); - Humidity: 60-65% (slightly lower than during the growth period to reduce the risk of contamination by miscellaneous bacteria); -Oxygen content: 20-21% (at normal atmospheric pressure, meeting basic breathing needs).

[0033] 2. Parameter settings for the ultrasonic stimulation component: - Frequency: 30-40kHz (low frequency for gentle stimulation, avoiding damage to unactivated bacteria); - Power: 50-80W (low power, only generates micro-disturbances, accelerating the contact of nutrients). - Stimulation mode: intermittent (on for 10 seconds, off for 30 seconds), with a cumulative stimulation time of 4 hours per day (divided into 8 sessions to avoid stress caused by continuous stimulation).

[0034] 3. Coordination Mechanism: -If the temperature sensor detects that the local temperature rises by ≥1℃ due to ultrasonic stimulation, the control component (16) automatically reduces the ultrasonic power by 10W and starts the micro cooling fan (new addition) of the environmental control component 15 to maintain temperature stability. - When the humidity is below 60%, first turn on the humidifier to replenish the humidity to the set value, and then resume ultrasonic stimulation (avoid ultrasonic stimulation damaging the mycelium in a dry environment).

[0035] (II) Rapid mycelial growth period (48-168h): Enhance synergy and promote efficient proliferation. 1. Environmental control component 15 parameter settings: -Temperature: 25-28℃ (the optimal growth temperature for Poria cocos mycelium, which enhances metabolic activity); - Humidity: 65-70% (high humidity to meet the water requirements for rapid mycelial growth); -Oxygen content: 21-23% (increase oxygen concentration to compensate for increased oxygen consumption caused by ultrasound stimulation).

[0036] 2. Parameter settings for the ultrasonic stimulation component: - Frequency: 40-60kHz (medium frequency, enhances cell membrane permeability and promotes nutrient absorption); - Power: 100-150W (medium power, utilizing cavitation effect to improve the fluidity of culture medium). - Stimulation mode: intermittent (on for 15 seconds, off for 25 seconds), with a cumulative stimulation of 8 hours per day (divided into 12 sessions, covering the active period of mycelium).

[0037] 3. Coordination Mechanism: When the oxygen sensor detects that the oxygen content is below 21%, the environmental control component 15 automatically increases the oxygen delivery volume, and at the same time switches the ultrasonic stimulation mode to "on for 10 seconds, off for 30 seconds" (to reduce the accelerated oxygen consumption caused by ultrasound) until the oxygen content rises. When the mycelial morphology sensor detects that the mycelial density has reached the threshold (≥80% culture medium coverage), the control component 16 automatically reduces the ultrasonic power by 20W to avoid excessive stimulation that could cause mycelial breakage.

[0038] (III) Sclerotium formation period (168-360h): Stable adaptation to ensure sclerotium development 1. Environmental control component 15 parameter settings: -Temperature: 24-26℃ (slightly lower than the growth period to promote mycelial aggregation and sclerotium formation); - Humidity: 60-65% (reduce humidity appropriately to prevent sclerotium rot); -Oxygen content: 19-20% (moderately reduce oxygen to induce sclerotium differentiation).

[0039] 2. Parameter settings for the ultrasonic stimulation component: - Frequency: 20-30kHz (low frequency, gently stimulates sclerotium development, avoids damage); - Power: 50-80W (low power, only maintains slight disturbance, promotes nutrient transport to sclerotia). - Stimulation mode: intermittent (on for 5 seconds, off for 45 seconds), 2 hours of stimulation per day (divided into 4 sessions to reduce interference with sclerotia).

[0040] 3. Coordination Mechanism: When the temperature sensor detects that the temperature is below 24°C, the environmental control component 15 starts the heating module and simultaneously stops ultrasonic stimulation (to avoid the dual inhibition of sclerotium formation by low temperature and ultrasound). - When the humidity is higher than 65%, first activate the dehumidification module to reduce it to the set value, and then resume ultrasonic stimulation (to prevent high humidity + ultrasound from causing sclerotia and mold growth).

[0041] III. Global feedback closed-loop collaboration (ensuring stability and accuracy); 1. Real-time sensor monitoring: - The incubator 11 is equipped with three types of environmental sensors (one set per level, 3-5 sets in total) for temperature, humidity and oxygen content, as well as a mycelial morphology sensor (which identifies mycelial density and sclerotium size through image recognition). - The ultrasonic component is equipped with a sound wave intensity sensor (to monitor the ultrasonic power density in each area, with a deviation of ≤5%).

[0042] 2. Dynamic adaptive adjustment: - The control component 16 receives sensor data in real time. If a parameter deviates from the set range (such as temperature ±0.5℃, humidity ±3%), the parameters of the environmental control component 15 are adjusted first. If the environmental parameters are stable but still do not meet the growth requirements, the ultrasonic parameters are finely adjusted (such as power ±10W, frequency ±5kHz). -Supports manual intervention: Users can modify the environmental / ultrasound parameters of a certain stage through control component 16. The system will automatically memorize and synchronously adjust the coordination logic (such as automatically increasing the oxygen content when the ultrasound power is manually increased).

[0043] IV. Core Innovations of Collaborative Control; 1. Stage-based matching: Based on the metabolic characteristics of different growth stages of Poria cocos strains, the precise binding of "environmental parameters - ultrasound parameters" is achieved, avoiding the "one-size-fits-all" universal regulation; 2. Feedback closed loop: Through real-time monitoring by multiple sensors, a "detection-judgment-adjustment" closed loop is formed to solve the problem of mutual interference between ultrasound stimulation and environmental changes; 3. Clear priorities: Environmental parameters are the foundation (ensuring strain survival), while ultrasound parameters are auxiliary (promoting proliferation). When the two conflict, environmental parameters should be stabilized first, and then ultrasound parameters should be adapted to ensure culture safety.

[0044] In summary, the present invention also has the following combined effects: This device, with "staged coordinated regulation + precise ultrasonic transmission" as its core, achieves efficient cultivation of Poria cocos strains through the coordinated operation of various components. The specific working logic is as follows: Core control logic: Based on the built-in database of Poria cocos strain growth stage parameters, the control component 16 automatically and collaboratively controls the temperature and humidity (22℃-28℃, 60%-85% relative humidity), oxygen concentration, light parameters, and frequency and power parameters of the ultrasonic stimulation component 15 according to the different growth cycles of the strain, so as to achieve precise matching between environmental conditions and ultrasonic stimulation.

[0045] Ultrasonic transmission path: The ultrasonic generator 12 outputs an electrical signal, which is converted into mechanical vibration by the electrically connected ultrasonic transducer 13; the transducer transmits the vibration to the ultrasonic transmission plate 26 through the coupling agent, and then through the detachable and coupling agent-filled ultrasonic transmission plate 25, it is transmitted to the integrally formed cylindrical mesh structure through the mesh transmission connector 211, and finally forms a uniform ultrasonic stimulation on the bottom and side wall of the culture dish 23.

[0046] Structural support and protection logic: The conduction frame 28 inside the culture dish 23 is detachably and stably installed through the inner wall support protrusion 27, ensuring the regularity of the cylindrical mesh structure; the vibration isolation base 24 is made of elastic damping materials such as silicone or nitrile rubber to block the transmission of vibration between adjacent culture dishes 23, and its opening 212 cooperates with the mesh bottom plate 14 in the incubator 11 to ensure the uniform flow of temperature, humidity and oxygen in the chamber; multiple sets of ultrasonic generators 12 correspond to different levels of the layered culture rack 21 to realize differentiated ultrasonic stimulation for each level.

[0047] The power supply module avoids power outages by combining solar panels with an external power supply module; the monitoring module collects data in real time from various sensors (temperature, humidity, oxygen concentration, light, etc.) and camera modules, and the control module 16 monitors and adjusts the equipment's operating status to ensure the stability of the cultivation process.

[0048] Uniform and efficient ultrasonic stimulation: The one-piece molded cylindrical mesh structure achieves full coverage of the culture dish 23, and the seamless conduction path design solves the problem of uneven stimulation in traditional equipment; the detachable and dockable conduction plate design balances ultrasonic conduction efficiency with convenient disassembly and maintenance. Strong multi-parameter synergy and adaptability: The staged parameter matching function of the control component 16 enables environmental parameters and ultrasonic parameters to be precisely adapted to the different growth needs of the strains. At the same time, multiple ultrasonic generators 12 correspond to multiple culture racks to achieve targeted stimulation, improve overall proliferation efficiency, and make up for the shortcomings of poor synergy and single parameters in traditional equipment. Stable and controllable culture environment: The environmental control component 15 is refined into four modules, and through components such as heating plates, semiconductor coolers, humidifiers, and micro air pumps, it achieves precise control of temperature, humidity, and oxygen concentration; the vibration isolation function of the vibration isolation base 24 and the flow function of the leakage holes 212 combine to avoid local environmental imbalance and interference from adjacent culture dishes 23, ensuring the consistency of the strain growth environment. Easy to operate and highly reliable: The touch screen of the control component 16 supports parameter input and real-time data display, and the built-in database enables automatic parameter matching, reducing human intervention errors; the detachable structure design facilitates later maintenance and cleaning, and the dual backup of the power supply component and the real-time monitoring of the monitoring component greatly improve the reliability of equipment operation and the repeatability of the cultivation process.

[0049] The embodiments of the present invention have been described above, but the present invention is not limited to the specific embodiments described above. The specific embodiments described above are merely illustrative and not restrictive. Those skilled in the art can make many other forms under the guidance of the present invention, all of which are within the protection scope of the present invention.

Claims

1. A device for ultrasonic stimulation and growth of Poria cocos strains, characterized in that, include: The incubator (11) and control components (16) are provided with a layered culture rack (21), an environmental control component (15) and an ultrasonic stimulation component. The ultrasonic stimulation component includes a conduction plate mesh (29) and a conduction enclosure mesh (210). The layered culture rack (21) is provided with several placement bases (22), and each placement base (22) is provided with a vibration isolation base (24). A culture dish (23) is placed on the vibration isolation base (24). A conductive plate mesh (29) is set at the bottom of the culture dish (23), and a conductive enclosure mesh (210) is set on the inner wall. The conductive plate mesh (29) and the conductive enclosure mesh (210) are enclosed to form a cylindrical mesh structure, which is used to uniformly stimulate the growth of the inside of the culture dish (23) with ultrasound. The outside of the culture dish (23) is provided with an ultrasonic transmission plate one (25), and the placement base (22) is provided with an ultrasonic transmission plate two (26); when the culture dish (23) is placed on the vibration isolation base (24), the ultrasonic transmission plate one (25) and the ultrasonic transmission plate two (26) are in a docking state and can transmit ultrasonic waves. The control component (16) is used to coordinate the temperature and humidity parameters, oxygen concentration parameters, and light parameters of the environmental control component (15) with the ultrasonic frequency parameters and power parameters of the ultrasonic stimulation component according to the different growth stages of the Poria cocos strain.

2. The ultrasonic stimulation growth device for Poria cocos strain according to claim 1, characterized in that, The ultrasonic stimulation assembly includes an ultrasonic generator (12) and an ultrasonic transducer (13). The ultrasonic generator (12) and the ultrasonic transducer (13) are electrically connected. The ultrasonic transducer (13) and the ultrasonic transmission plate two (26) are connected by a coupling agent to realize the transmission of ultrasonic waves. The ultrasonic transmission plate one (25) and the ultrasonic transmission plate two (26) are detachably connected and a coupling agent is provided between them.

3. The ultrasonic stimulation growth device for Poria cocos strain according to claim 2, characterized in that, The side of the culture dish (23) is fitted with a mesh conduction connector (211), which is used to connect the ultrasonic conduction plate (25) with the conduction plate mesh (29) and the conduction enclosure mesh (210).

4. The ultrasonic stimulation growth device for Poria cocos strain according to claim 3, characterized in that, The conductive plate mesh (29) and the conductive enclosure mesh (210) are integrally formed, and the mesh conductive connector (211) is fixedly connected to the cylindrical mesh structure enclosed by the conductive plate mesh (29) and the conductive enclosure mesh (210).

5. The ultrasonic stimulation growth device for Poria cocos strain according to claim 4, characterized in that, The ultrasonic generator (12) is configured in multiple groups, and the layered culture rack (21) is configured as a multi-layer structure. The multiple groups of ultrasonic generators (12) are respectively connected to different layers of the layered culture rack (21) to apply differentiated ultrasonic frequencies and power stimulation to different layers of the layered culture rack (21).

6. The ultrasonic stimulation growth device for Poria cocos strain according to claim 1, characterized in that, The culture dish (23) is provided with a conductive frame (28) inside. The conductive frame (28) is used to install the conductive plate mesh (29) and the conductive enclosure mesh (210). The vibration isolation base (24) is provided with a leakage hole (212).

7. The ultrasonic stimulation growth device for Poria cocos strain according to claim 6, characterized in that, The inner wall of the culture dish (23) is provided with a support protrusion (27), which is used to support the conduction frame (28), and the conduction frame (28) is detachably installed inside the culture dish (23).

8. The ultrasonic stimulation growth device for Poria cocos strain according to claim 7, characterized in that, The bottom of the incubator (11) is provided with a mesh bottom plate (14) for placing the layered culture rack (21). The environmental control components include a temperature control module, a humidity control module, an oxygen control module and a light control module.

9. The ultrasonic stimulation growth device for Poria cocos strain according to claim 8, characterized in that, The incubator (11) is equipped with a power supply component and a monitoring component. The power supply component includes a solar panel and an external power supply module. The monitoring component includes a temperature sensor, a humidity sensor, an oxygen concentration sensor, a light sensor, and a camera module. The control component (16) is used to control the environmental control component, the ultrasonic stimulation component, the power supply component, and the monitoring component.

10. The ultrasonic stimulation growth device for Poria cocos strain according to claim 9, characterized in that, The control component (16) includes a controller, a touch screen and multiple sensors. The controller is electrically connected to the ultrasonic stimulation component and the environmental control component respectively. The temperature sensor, humidity sensor, oxygen concentration sensor and light sensor are embedded in the incubator body and are used to collect environmental parameters inside the incubator in real time.

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