Method for dissolving poorly water-soluble protein, protein solution obtained thereby, and molded article thereof

Abstract

Provided are a method for dissolving a poorly water-soluble protein, and a molded article produced using a protein solution obtained thereby. The present invention is a method for dissolving a poorly water-soluble protein, the method comprising a step for mixing a hydroxyl group-containing polymer, a poorly water-soluble protein, and an ionic liquid. In addition, the present invention makes it possible to produce a molded article using the poorly water-soluble protein solution obtained by the method.

Description

Technical Field

[0001] This invention relates to a method for dissolving water-insoluble proteins and the resulting protein solution, and more particularly to a method for dissolving a hydroxyl-containing polymer and a water-insoluble protein in an ionic liquid, the resulting water-insoluble protein solution, and the molded form thereof. Background Technology

[0002] In recent years, the Sustainable Development Goals (SDGs) have received much attention. To move towards a sustainable society, people are striving to effectively utilize previously discarded resources and maintain a bountiful environment without damaging the planet. As part of this effort, there is a desire to combine water-insoluble proteins—difficult-to-recycle natural products—with hydroxyl-containing polymers, also natural products, to create a homogeneous solution, which can then be reused as fibers and / or film-like molded products. Summary of the Invention

[0003] Methods for solving problems In addition to effectively utilizing waste resources, the inventors of this invention have discovered a method for dissolving water-insoluble proteins to achieve recycling. Furthermore, they have also discovered a method for extracting and solvating water-insoluble proteins in their high molecular weight form without hydrolysis to reduce their molecular weight, thereby exhibiting new functions. Therefore, this invention provides a method for dissolving water-insoluble proteins using ionic liquids.

[0004] According to the main points of the present invention, the following invention is provided: (Project 1) A method for dissolving a water-insoluble protein, comprising a step of mixing a hydroxyl-containing polymer, a water-insoluble protein, and an ionic liquid.

[0005] (Project 2) The method according to any of the foregoing items further includes a step of mixing a co-solvent.

[0006] (Project 3) According to the method described in any of the foregoing items, the cosolvent is a surfactant.

[0007] (Project 4) According to the method described in any of the foregoing projects, the surfactant is a cationic surfactant with a superacid as its counterion.

[0008] (Project 5) According to the method described in any of the foregoing items, when the total weight of the above-mentioned water-insoluble protein and hydroxyl-containing polymer is 100% by weight, the content of the above-mentioned surfactant is about 0.05% by weight to about 10% by weight.

[0009] (Project 6) According to the method described in any of the foregoing items, when the total weight of the above-mentioned water-insoluble protein and the hydroxyl-containing polymer is 100% by weight, the content of the above-mentioned water-insoluble protein is about 5% by weight to about 90% by weight.

[0010] (Project 7) According to the method described in any of the foregoing projects, the aforementioned water-insoluble proteins include eggshell membranes, wool, chicken feathers, silk, and scales, as well as their partially hydrolyzed products and / or partially reduced products.

[0011] (Project 8) According to the method described in any of the foregoing projects, the ionic liquid comprises imidazolium cation, pyridinium cation, and alkylammonium cation.

[0012] (Project 9) According to the method described in any of the foregoing items, the hydroxyl-containing polymer includes a modified hydroxyl-containing polymer on which functional functional groups are incorporated.

[0013] (Project 10) According to the method described in any of the foregoing items, the hydroxyl-containing polymer includes saponifications of cellulose, polyvinyl alcohol, and polyvinyl acetate.

[0014] (Project 11) A solution obtained by dissolving a hydroxyl-containing polymer and a water-insoluble protein in an ionic liquid.

[0015] (Project 12) The solution according to any of the foregoing items further comprises a co-solvent.

[0016] (Project 13) The solvent according to any of the foregoing items, wherein the co-solvent is a surfactant.

[0017] (Project 14) According to the solution described in any of the foregoing items, the surfactant is a cationic surfactant with a superacid as its opposite ion.

[0018] (Project 15) According to the solution described in any of the foregoing items, when the total weight of the above-mentioned water-insoluble protein and hydroxyl-containing polymer is 100% by weight, the content of the above-mentioned surfactant is about 0.05% by weight to about 10% by weight.

[0019] (Project 16) According to the solution described in any of the foregoing items, when the total weight of the above-mentioned water-insoluble protein and the hydroxyl-containing polymer is 100% by weight, the content of the above-mentioned water-insoluble protein is about 5% by weight to about 90% by weight.

[0020] (Project 17) According to the solutions described in any of the foregoing items, the aforementioned water-insoluble proteins include eggshell membranes, wool, chicken feathers, silk, and scales, as well as their partially hydrolyzed products and / or partially reduced products.

[0021] (Project 18) The solution according to any of the foregoing items, wherein the ionic liquid comprises imidazolium cation, pyridinium cation and alkylammonium cation.

[0022] (Project 19) The solution according to any of the foregoing items, wherein the hydroxyl-containing polymer includes a modified hydroxyl-containing polymer on which functional functional groups are incorporated.

[0023] (Project 20) The solution according to any of the foregoing items, wherein the hydroxyl-containing polymers include saponifications of cellulose, polyvinyl alcohol and polyvinyl acetate.

[0024] (Project 21) A method for manufacturing a polymer molded body containing a water-insoluble protein includes: a step of providing an ionic liquid in which the water-insoluble protein and a hydroxyl-containing polymer are dissolved; and a step of precipitating the polymer molded body from the ionic liquid.

[0025] (Project 22) According to the method described in any of the foregoing items, the provision of the aforementioned ionic liquid is carried out through the following steps: (a) The process of mixing water-insoluble proteins with ionic liquids; (b) The process of mixing a hydroxyl-containing polymer with an ionic liquid; and (c) The process of mixing the protein solution obtained from step (a) with the polymer solution obtained from step (b).

[0026] (Project 23) According to the method described in any of the foregoing items, the ionic liquid is provided by a process of mixing a water-insoluble protein and a hydroxyl-containing polymer into the ionic liquid in a one-time or sequential manner.

[0027] (Project 23A) A method for manufacturing a polymer molded article containing a water-insoluble protein, comprising: (a) The process of mixing water-insoluble proteins with ionic liquids; (b) The process of mixing a hydroxyl-containing polymer with an ionic liquid; (c) The process of mixing the protein solution obtained in step (a) with the polymer solution obtained in step (b); and (d) The process of precipitating polymer molded articles from the solution obtained by process (c).

[0028] (Project 23B) A method for manufacturing a polymer molded article containing a water-insoluble protein, comprising: (a') A process of mixing water-insoluble proteins and hydroxyl-containing polymers into an ionic liquid in a single step or sequentially; and (b') The process of precipitating polymer molded articles from the solution obtained by process (a').

[0029] (Project 24) According to the method described in any of the foregoing projects, a co-solvent is further mixed into the aforementioned ionic liquid.

[0030] (Project 25) According to the method described in any of the foregoing projects, the aforementioned water-insoluble proteins include eggshell membranes, wool, chicken feathers, silk, and scales, as well as their partially hydrolyzed products and / or partially reduced products.

[0031] (Project 26) According to the method described in any of the foregoing projects, the ionic liquid comprises imidazolium cation, pyridinium cation, and alkylammonium cation.

[0032] (Project 27) According to the method described in any of the foregoing items, the hydroxyl-containing polymer includes a modified hydroxyl-containing polymer on which functional functional groups are incorporated.

[0033] (Project 28) According to the method described in any of the foregoing items, the hydroxyl-containing polymer includes saponifications of cellulose, polyvinyl alcohol, and polyvinyl acetate.

[0034] (Project 29) A method for manufacturing a polymer molded article containing a water-insoluble protein includes a step of extruding the solution described in any of the preceding items into a poor solvent containing a hydroxyl-containing polymer and a water-insoluble protein.

[0035] (Project 30) A polymer molded article containing a water-insoluble protein, the molded article being obtained by a method comprising the following steps: A process for dissolving ionic liquids containing poorly soluble water-soluble proteins and hydroxyl-containing polymers; and The process of precipitating polymer molded articles from the ionic liquid.

[0036] (Project 31) According to the molded body described in any of the foregoing items, the provision of the aforementioned ionic liquid is carried out through the following steps: (a) The process of mixing water-insoluble proteins with ionic liquids; (b) The process of mixing a hydroxyl-containing polymer with an ionic liquid; and (c) The process of mixing the protein solution obtained from step (a) with the polymer solution obtained from step (b).

[0037] (Project 32) According to any of the foregoing items, the provision of the ionic liquid is carried out by a process of mixing a water-insoluble protein and a hydroxyl-containing polymer into the ionic liquid in a one-time or sequential manner.

[0038] (Project 30A) A polymer molded article containing a water-insoluble protein, the molded article being obtained by a method comprising the following steps: (a) The process of mixing water-insoluble proteins with ionic liquids; (b) The process of mixing a hydroxyl-containing polymer with an ionic liquid; (c) The process of mixing the protein solution obtained in step (a) with the polymer solution obtained in step (b); and (d) The process of precipitating polymer molded articles from the solution obtained by process (c).

[0039] (Project 30B) A polymer molded article containing a water-insoluble protein, the molded article being obtained by a method comprising the following steps: (a') A process of mixing water-insoluble proteins and hydroxyl-containing polymers into an ionic liquid in a single step or sequentially; and (b') The process of precipitating polymer molded articles from the solution obtained by process (a').

[0040] (Project 33) The molded body according to any of the foregoing items includes fibers, yarns, fabrics, knitted fabrics, woven fabrics, nonwoven fabrics, and films.

[0041] (Project 34) A kit for manufacturing polymer molded articles containing water-insoluble proteins, comprising an ionic liquid, a hydroxyl-containing polymer, and a water-insoluble protein.

[0042] (Project 35) The kit according to any of the foregoing items further comprises a co-solvent.

[0043] (Project 36) According to the kit described in any of the foregoing items, the cosolvent is a surfactant.

[0044] (Project 37) According to the kit described in any of the foregoing items, the surfactant is a cationic surfactant with a superacid as its opposite ion.

[0045] (Project 38) According to the kit described in any of the foregoing items, when the total weight of the above-mentioned water-insoluble protein and hydroxyl-containing polymer is 100% by weight, the content of the above-mentioned surfactant is about 0.05% by weight to about 10% by weight.

[0046] (Project 39) According to the kit described in any of the foregoing items, when the total weight of the above-mentioned poorly soluble protein and the hydroxyl-containing polymer is 100% by weight, the content of the above-mentioned poorly soluble protein is about 5% by weight to about 90% by weight.

[0047] (Project 40) According to the kit described in any of the foregoing items, the aforementioned water-insoluble proteins include eggshell membranes, wool, chicken feathers, silk, and scales, as well as their partially hydrolyzed products and / or partially reduced products.

[0048] (Project 41) According to the kit described in any of the foregoing items, the ionic liquid comprises imidazolium cation, pyridinium cation, and alkylammonium cation.

[0049] (Project 42) According to the kit described in any of the foregoing items, the hydroxyl-containing polymer includes a modified hydroxyl-containing polymer on which functional functional groups are incorporated.

[0050] (Project 43) According to the kit described in any of the foregoing items, the hydroxyl-containing polymers include saponifications of cellulose, polyvinyl alcohol, and polyvinyl acetate.

[0051] In this disclosure, it is contemplated that one or more of the features described above may be further combined in addition to the disclosed combinations. It should be noted that those skilled in the art can identify further embodiments and advantages of this disclosure by reading and understanding the following detailed description.

[0052] It should be noted that, apart from the above, the features and significant effects of this disclosure can be clearly understood by those skilled in the art by referring to the following embodiments and accompanying drawings.

[0053] This invention enables excellent and effective utilization of water-insoluble protein resources such as eggshell membranes, chicken feathers, wool, and scales, which were previously often discarded, and can generate protein solutions that can easily produce molded bodies with new functions.

[0054] In addition to their original properties, the protein solution and its molded form of the present invention can also be endowed with new functions, thus possessing functions such as hygroscopicity, deodorization, and antibacterial properties. Attached Figure Description

[0055] Figure 1 This is a cross-section of spun cotton spun using the protein solution generated in Example 2; Figure 2 This is an electron microscope (SEM) image of the cross-section of spun cotton spun using the protein solution generated in Example 11; Figure 3 This is a cross-section of spun cotton spun using the protein solution generated in Comparative Example 2, as shown by electron microscopy (SEM). Detailed Implementation

[0056] The present disclosure will now be described with reference to its preferred form. It should be understood that throughout the scope of this specification, unless otherwise indicated, the singular form includes the concept of its plural form. Therefore, it should be understood that, unless otherwise indicated, articles in the singular form (e.g., "a," "an," "the," etc. in English) also include the concept of their plural forms. Furthermore, it should be understood that, unless otherwise indicated, the terms used in this specification are used in their usual meaning in the art. Therefore, unless otherwise defined, all terms and technical terms used in this specification have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. In the event of inconsistency, this specification (including definitions) takes precedence.

[0057] The following provides appropriate definitions of terms and / or basic technical content used in this specification.

[0058] In this specification, “about” means ±10% of the value that follows.

[0059] In this specification, "poorly soluble protein" refers to a protein that does not dissolve into a clear solution even when heated at approximately 80°C for approximately 3 hours in an aqueous solution of approximately 0.1% by weight. As a method for determining whether a protein is poorly soluble in water, conventional methods can be used to confirm whether the above conditions are met. Examples of poorly soluble proteins include eggshell membranes, wool, chicken feathers, scales, and silk.

[0060] In this specification, "partially hydrolyzed products" of poorly soluble proteins refer to the hydrolysates of poorly soluble proteins where hydrolysis ceases in the high molecular weight regions. Partially hydrolyzed products may have a weight-average molecular weight of approximately 10,000 or more. Furthermore, in this specification, "partially reduced products" of poorly soluble proteins refer to the reduced products of poorly soluble proteins where reduction ceases in the high molecular weight regions. Partially reduced products may have a weight-average molecular weight of approximately 10,000 or more.

[0061] In this specification, the term "protein solution" obtained by dissolving water-insoluble proteins should be interpreted broadly, mainly referring to a solution in which water-insoluble proteins have been dissolved.

[0062] In this specification, "polymer molded body" should be interpreted broadly as a molded body whose main component is a polymer.

[0063] In this specification, "hydroxyl-containing polymer" should be interpreted broadly to refer to high molecular weight compounds containing hydroxyl groups. Examples of hydroxyl-containing polymers include polysaccharides such as starch, glycogen, cellulose, chitin, agarose, hyaluronic acid, chondroitin sulfate, pectin, and carrageenan; polyvinyl alcohol (PVA); and synthetic polymers such as phenolic resins and polyvinyl acetate. Furthermore, partially saponified forms of regenerated cellulose and / or polyvinyl acetate may also be listed as hydroxyl-containing polymers.

[0064] In this specification, "high shear force" mixing refers to mixing liquids or liquids and solids by shearing them simultaneously through high-speed rotating stirring blades. Examples of mixers capable of achieving high shear force include high-speed shear mixers such as homogenizers, turbine mixers, and bio-mixers, as well as high-pressure homogenizers, ultrasonic homogenizers, and in-tube mixers (including static mixers and injection molding mixers (single-shaft and dual-shaft)).

[0065] In this specification, the term "vibrational mixing" should be interpreted broadly as stirring while vibrating the liquid or solid containing the mixture in a container. Vibrational mixing can be achieved, for example, through stirring based on a stirrer, stirring based on ultrasonic vibration, or stirring based on molecular-level vibration.

[0066] In this specification, "dissolved" states include states where the solution appears transparent, translucent, or emulsified.

[0067] (Preferred Implementation) The following describes preferred embodiments of this disclosure. The embodiments provided below are intended to better understand this disclosure, and the scope of this disclosure should not be limited to the following description. Therefore, those skilled in the art will readily recognize that appropriate modifications can be made within the scope of this disclosure with reference to the description herein. Furthermore, the following embodiments of this disclosure can be used alone or in combination.

[0068] In one aspect of the invention, a method for dissolving a poorly soluble protein is provided, comprising the steps of mixing a hydroxyl-containing polymer, a poorly soluble protein, and an ionic liquid. In one embodiment of the invention, a solution of a poorly soluble protein can be obtained by mixing an ionic liquid containing a dissolved hydroxyl-containing polymer with the poorly soluble protein, or by combining and mixing the hydroxyl-containing polymer and the poorly soluble protein into an ionic liquid. In another embodiment, a solution of a poorly soluble protein can also be obtained by adding a hydroxyl-containing polymer after mixing the poorly soluble protein into the ionic liquid.

[0069] [Water-insoluble proteins] In one embodiment, the method of the present invention can obtain a water-insoluble protein solution by mixing a water-insoluble protein into an ionic liquid.

[0070] In one embodiment, ionic liquids composed of the following cations and anions can be cited as examples.

[0071] Examples of cations that constitute ionic liquids include imidazolium cations (a11), pyridinium cations (a12), and alkylammonium cations (a13), but are not limited to these.

[0072] Examples of imidazolium cations (a11) include, but are not limited to, N-methylimidazolium, N-ethylimidazolium, 1,3-dimethylimidazolium, 1,3-diethylimidazolium, 1-ethyl-3-methylimidazolium, 1-propyl-3-methylimidazolium, 1-butyl-3-methylimidazolium, 1-hexyl-3-methylimidazolium, 1,2,3-trimethylimidazolium, and 1,2,3,4-tetramethylimidazolium.

[0073] Examples of pyridinium cations (a12) include, but are not limited to, N-propylpyridinium, N-butylpyridinium, 1,4-dimethylpyridinium, 1-butyl-4-methylpyridinium, and 1-butyl-2,4-dimethylpyridinium.

[0074] Examples of alkylammonium cations (a13) include trimethylammonium, ethyldimethylammonium, diethylmethylammonium, triethylammonium, tetramethylammonium, triethylmethylammonium, tetraethylammonium, etc., with triethylmethylammonium cation and tetraethylammonium cation being preferred.

[0075] In one embodiment, the cation constituting the ionic liquid can be any one of (a11) to (a13), or a combination of two or more, or even any one of (a11) to (a13) or two or more used together.

[0076] From the viewpoint of solubility, imidazolium cation (a11) and pyridinium cation (a12) are preferred, with imidazolium cation (a11) being even more preferred.

[0077] Examples of anions constituting ionic liquids include halogen anions, carboxylate anions, sulfonate anions, and phosphate anions. In one embodiment, halogen anions include chloride anions, bromide anions, and iodide anions.

[0078] As carboxylate anions, examples include monocarboxylate anions and dicarboxylate anions with 1 to 18 carbon atoms, such as formate anion, acetate anion, lactate anion, oxalate anion, fumarate anion, adipic acid anion, and pyruvate anion.

[0079] Examples of sulfonate anions include sulfonate anion, methanesulfonate anion, octanesulfonate anion, dodecanesulfonate anion, and eicosanesulfonate anion.

[0080] Examples of phosphate anions include phosphate anion, methyl phosphate monoester anion, ethyl phosphate monoester anion, propyl phosphate monoester anion, butyl phosphate monoester anion, methyl phosphate diester anion, ethyl phosphate diester anion, propyl phosphate diester anion, and butyl phosphate diester anion.

[0081] In one embodiment, from the viewpoint of solubility, the anions constituting the ionic liquid are preferably halogen anions, carboxylate anions, and phosphate anions, and more preferably carboxylate anions and halogen anions.

[0082] In one embodiment, from the viewpoint of solubility and orientation, an ionic liquid that is liquid at temperatures below 100°C is preferred. In another embodiment, from the viewpoint of operability, it is further preferred that the ionic liquid (A) itself is liquid at 25°C (room temperature).

[0083] Examples of such ionic liquids include imidazolium carboxylates and imidazolium chlorides, with 1-ethyl-3-methylimidazolium carboxylate, 1-ethyl-3-methylimidazolium acetate, 1-ethyl-2,3-dimethylimidazolium acetate, 1-butyl-3-methylimidazolium acetate, 1-ethyl-3-methylimidazolium fumarate, 1-ethyl-3-methylimidazolium lactate, 1-butyl-3-methylimidazolium chloride, and 1-ethyl-3-methylimidazolium chloride being particularly preferred.

[0084] The ionic liquid of the present invention may further comprise water, lower alcohols, and / or glycols. In one embodiment, the content of water, lower alcohols, and / or glycols in the ionic liquid is preferably less than 10% by weight.

[0085] In one embodiment of the invention, eggshell membranes are cited as an example of water-insoluble proteins. The source of the eggshell membrane, which is a water-insoluble protein dissolved by the method of the present invention, is not particularly limited. For example, eggshell membranes from poultry such as chickens, quails, turkeys, wild ducks, domestic ducks, geese, ostriches, helmeted guinea fowl, long-tailed chickens, dwarf chickens, pigeons, mute swans, emus, or pheasants can be used. In one embodiment of the invention, chickens are preferred from the perspectives of easy availability, high egg production, large egg size, and established large-scale breeding methods.

[0086] Eggshell membranes typically have eggshells attached to them. If these membranes are dissolved directly through hydrolysis or other methods, the eggshells will remain in an insoluble state and will be present as foreign matter when the molded product is obtained, which is therefore not preferable. To remove the eggshells, it is preferable to soak the eggshell membrane containing the eggshells in an aqueous solution of hydrochloric acid, phosphoric acid, etc., so that the eggshells dissolve in the water, and then remove them in advance by filtration and washing.

[0087] In one embodiment of the present invention, anionic surfactants, cationic surfactants, amphoteric surfactants, and nonionic surfactants can be listed as surfactants. In one embodiment of the present invention, when a hydroxyl-containing polymer and a water-insoluble protein are uniformly dissolved and molded into a film or fiber, the orientation of the protein and polymer components can be improved regardless of the ionicity of the surfactant, thereby producing a molded article with excellent physical properties such as strength.

[0088] In one embodiment of the present invention, from the viewpoint of solubility during dissolution and orientation during molding, cationic surfactants, amphoteric surfactants and nonionic surfactants are preferred, and cationic surfactants are even more preferred.

[0089] In this specification, "cosolvent" refers to a substance that promotes the dissolution of a substance in a solution. Examples of cosolvents include surfactants, acid-base agents, and redox agents.

[0090] Where it is undesirable for surfactant residues to remain in the final molded body, water-soluble surfactants are preferred; conversely, where it is desirable for the surfactant to function in the molded body, surfactants with lower HLB (hydrophilic-lipophilic balance) values ​​are preferred. In one embodiment, the HLB of the surfactant used in the method of the present invention can be about 20 or less, about 19 or less, about 18 or less, about 17 or less, about 16 or less, about 15 or less, about 14 or less, about 13 or less, about 12 or less, about 11 or less, about 10 or less, about 9 or less, about 8 or less, about 7 or less, about 6 or less, or about 5 or less. Particularly in the case of cationic surfactants, it is preferable to increase the content of cationic surfactants remaining in the final molded body and enable them to function by making the opposite ion a superacid, thus making it poorly soluble in water.

[0091] In this specification, "superacid" refers to an acid with a stronger acidity than 100% sulfuric acid by weight. Examples of conjugate anions of superacids include fluorosulfonic acid, trifluoromethanesulfonic acid, and trifluoroboric acid.

[0092] [Pulverization of water-insoluble proteins] In one embodiment, the water-insoluble protein can be pulverized to facilitate its dissolution. In another embodiment, there is no particular limitation on the diameter of the water-insoluble protein during pulverization. For example, in the case of pulverization, the diameter of the water-insoluble protein can be approximately 0.1 μm or more, approximately 0.2 μm or more, approximately 0.3 μm or more, approximately 0.4 μm or more, approximately 0.5 μm or more, approximately 0.6 μm or more, approximately 0.7 μm or more, approximately 0.8 μm or more, approximately 0.9 μm or more, approximately 1.0 μm or more, approximately 1.5 μm or more, approximately 2.0 μm or more, approximately 3.0 μm or more, approximately 4.0 μm or more, approximately 4.0 μm or more, or approximately 5.0 μm or more, and its diameter can be approximately 5 mm or less, approximately 4 mm or less, approximately 3 mm or less, approximately 2 mm or less, or approximately 1 mm or less. In one embodiment, when the protein is pulverized, the diameter of the poorly soluble protein is preferably about 0.1 μm or more and about 5 mm or less, more preferably about 1 μm or more and about 1 mm or less. Within this range, when the poorly soluble protein is prepared into a solution using the method of the present invention, it can be dissolved uniformly.

[0093] [Moisture content of water-insoluble proteins] In one embodiment, to facilitate the dissolution of water-insoluble proteins, the moisture content of the water-insoluble proteins can be specified. In one embodiment, where water washing is performed as a pretreatment to facilitate the dissolution of water-insoluble proteins, the moisture content can be about 80% by weight or less, about 70% by weight or less, about 60% by weight or less, about 50% by weight or less, about 40% by weight or less, about 30% by weight or less, about 20% by weight or less, or about 10% by weight. It is preferable to achieve a moisture content of about 5% by weight or less, about 4% by weight or less, about 3% by weight or less, about 2% by weight or less, or about 1% by weight or less through freeze drying or the like.

[0094] [Dissolution of water-insoluble proteins] In one embodiment, the method of the present invention can dissolve a water-insoluble protein by mixing it with an ionic liquid, for example by applying high shear force and / or vibration to the mixed solution of the water-insoluble protein and the ionic liquid.

[0095] In one embodiment, when dissolving water-insoluble proteins, the concentration of the water-insoluble proteins is not particularly limited, but is preferably from about 5% to about 50% by weight, for example, about 5% by weight, about 10% by weight, about 15% by weight, about 30% by weight, or about 50% by weight.

[0096] In one embodiment, the temperature of the solution is not particularly limited when dissolving water-insoluble proteins, but is preferably about 40°C or higher, more preferably about 45°C or higher, 50°C or higher, about 55°C or higher, 65°C or higher, about 70°C or higher, 75°C or higher, about 80°C or higher, or about 90°C or higher.

[0097] In one embodiment, when dissolving a water-insoluble protein, the solution formed by mixing the water-insoluble protein and the ionic liquid can be stirred using conventional methods, such as a known mixer. In another embodiment, the solution formed by mixing the water-insoluble protein and the ionic liquid can be stirred using a high-viscosity mixer and / or a homogenizer, or an ultrasonic mixer, etc.

[0098] In one embodiment, in the method of the present invention, the water-insoluble protein can be pulverized into a diameter as described above, and then dissolved by mixing with an ionic liquid. In the prior art, to prepare a solution of water-insoluble proteins, they are dissolved by hydrolysis to reduce their molecular weight. Protein hydrolysis is carried out by acids (hydrochloric acid, sulfuric acid, etc.), bases (sodium hydroxide, potassium hydroxide, etc.), or proteolytic enzymes (microbial enzymes, plant-derived enzymes, animal-derived enzymes). For example, high-temperature hydrolysis in the presence of an alkali has been proposed to produce soluble proteins with relatively large molecular weights (see, for example, Patent 6288686). Furthermore, protein hydrolysates with high cysteine ​​content have been proposed to allow proteolytic enzymes with optimal pH values ​​in alkaline regions to function (see, for example, Japanese Patent Application Publication No. 2018-177713).

[0099] In addition, a technique has been proposed to produce a fiber assembly by electrospinning a protein dissolved in thiopropionic acid or the like (see, for example, Japanese Patent Application Publication No. 2009-89859 and Japanese Patent No. 5166953). Furthermore, a method has been proposed to process soluble proteins that have been hydrolyzed in the presence of a reducing agent into fiber preforms (see, for example, Japanese Patent No. 4387806 and Japanese Patent Application Publication No. 2004-84154).

[0100] However, in the alkaline hydrolysis described in Japanese Patent 6288686, a neutralization step with an acidic substance is required to stop the decomposition reaction, which results in a decrease in the cysteine ​​content of the protein during the neutralization process. Furthermore, in the enzymatic hydrolysis described in Japanese Patent Application Publication No. 2018-177713, it is difficult to obtain soluble components of large molecular weight proteins, and the protein-degrading enzymes used as catalysts are expensive, thus increasing manufacturing costs.

[0101] Furthermore, the acid-based hydrolysis method has the following problems: the apparatus is prone to corrosion; the protein is excessively decomposed, deviating from the target molecular weight distribution and becoming too low in molecular weight; it is colored brown; and byproducts are easily generated in addition to the target hydrolysis products. Moreover, in the fiber manufacturing method described in Japanese Patent Application Publication No. 2009-89859 and / or Japanese Patent No. 5166953, after dissolving the protein in acid, spinning is performed using a special apparatus, but a natural protein structure is not formed in the fiber aggregate. Additionally, in the films and / or sheets described in Japanese Patent No. 4387806 and / or Japanese Patent Application Publication No. 2004-84154, the use of hydrolyzed soluble protein limits its application to wound dressing materials such as adhesive bandages.

[0102] Furthermore, in Japanese Patent 4070579, a method for utilizing water-insoluble proteins was proposed to introduce functional components into viscose rayon. The method involves dissolving water-insoluble proteins such as animal hair at pH 8-12, mixing the cross-linked protein (which has been cross-linked in an alkaline aqueous solution using a water-soluble cross-linking agent) with viscose, and then spinning the mixture to form the functional rayon. However, in this method, the product of alkaline hydrolysis of the protein is also cross-linked with a cross-linking agent, making it different from the original protein, similar to other prior art methods.

[0103] Furthermore, in Japanese Patent 5071613, a method was proposed to allow water-insoluble active ingredients (such as titanium dioxide and / or zinc oxide) to adhere to the surface of a tooth: the water-insoluble active ingredient is dissolved in an aqueous solution containing an electrolyte by using an amphoteric polymer compound having both anionic and cationic dissociative groups in one molecule, and / or a combination of anionic and cationic polymer compounds. However, water-insoluble proteins are not completely dissolved in this technique.

[0104] In one embodiment of the present invention, the method according to the invention can dissolve water-insoluble proteins while avoiding these problems. Therefore, the protein solution obtained by dissolving water-insoluble proteins using the method of the present invention can possess the original structure and / or properties of the natural protein. Furthermore, since non-hydrolyzed or partially hydrolyzed water-insoluble proteins can be used, molding such as fibrosis can be performed at high molecular weights, increasing the strength of the molded body. In addition, the protein content can be increased; for example, when using eggshell membrane as a water-insoluble protein, since the fibrous morphology is preserved intact, structural characteristics such as increased moisturizing properties, deodorizing properties, and cell proliferation can be expected.

[0105] In one embodiment, in the method of the present invention, the water-insoluble protein may be used without hydrolysis or reduction treatment, or a water-insoluble hydrolysate (partially hydrolyzed product) obtained by partially hydrolyzing the water-insoluble protein may be used, or a water-insoluble reduced product (partially reduced product) obtained by partially reducing the water-insoluble protein may be used. Conventional hydrolysis and / or reduction methods such as alkaline hydrolysis, reducing agent treatment, and enzymatic hydrolysis can be used as methods for hydrolysis and / or reduction treatment. For example, when using eggshell membrane as a water-insoluble protein, to partially hydrolyze the eggshell membrane, a substance can be used that hydrolyzes the eggshell membrane in an approximately 5% alkaline aqueous solution at approximately 60°C for approximately 1 hour, neutralizes it with hydrochloric acid of the appropriate alkaline equivalent, and then washes and dries the precipitated hydrolysate. Even when using other water-insoluble proteins such as wool and / or feathers, the same partially hydrolyzed product can be obtained by adjusting the alkaline concentration, hydrolysis temperature, time, etc. In one embodiment, the water-insoluble hydrolysate (partially hydrolyzed product) obtained by partially hydrolyzing the water-insoluble protein or the water-insoluble reduced product (partially reduced product) obtained by partially reducing the water-insoluble protein can have a weight average molecular weight of about 10,000 or more, but is not limited thereto.

[0106] [Pulverization of hydroxyl-containing polymers] In one embodiment, to facilitate the dissolution of the hydroxyl-containing polymer, the polymer can be pulverized. In one embodiment, there is no particular limitation on the diameter of the pulverized polymer; for example, by mechanically pulverizing it into a cotton-like form using a juicer, the hydroxyl-containing polymer can be uniformly dissolved when preparing a solution using the method of the present invention.

[0107] [Moisture content of hydroxyl-containing polymers] In one embodiment, to facilitate the dissolution of water-insoluble proteins, the moisture content of the water-insoluble proteins can be specified. In one embodiment, to facilitate the dissolution of hydroxyl-containing polymers, in the case of pretreatment with water washing, the moisture content can be about 80% by weight or less, about 70% by weight or less, about 60% by weight or less, about 50% by weight or less, about 40% by weight or less, about 30% by weight or less, and preferably about 20% by weight or less, about 10% by weight or less, or about 5% by weight or less, obtained by using a hot air dryer or the like.

[0108] [Dissolution of hydroxyl-containing polymers] In one embodiment, in the method of the present invention, the hydroxyl-containing polymer is swollen by mixing it with an aqueous ionic liquid, and then completely dissolved by dehydration. As the aqueous ionic liquid, an ionic liquid with a water content of about 50% by weight or less, about 40% by weight or less, or about 30% by weight or less can be used. From the viewpoint of the swelling properties and dehydration efficiency of the hydroxyl-containing polymer, it is preferable to swell the hydroxyl-containing polymer in an aqueous ionic liquid with a water content of about 10% to about 30%, and then dehydrate it to a water content of about 15% by weight or less, preferably about 10% by weight or less, thereby dissolving the hydroxyl-containing polymer.

[0109] In one embodiment, the concentration of the hydroxyl-containing polymer is not particularly limited when dissolving it, but is preferably about 5% to about 30% by weight, such as about 5% by weight, about 10% by weight, about 15% by weight, or about 20% by weight.

[0110] In one embodiment, the temperature of the solution when dissolving the hydroxyl-containing polymer is not particularly limited, but is preferably about 40°C or higher, more preferably about 45°C or higher, 50°C or higher, about 55°C or higher, about 65°C or higher, about 70°C or higher, 75°C or higher, about 80°C or higher, or about 90°C or higher.

[0111] [Protein solution of water-insoluble proteins] In one aspect of the invention, a protein solution obtained by dissolving a poorly soluble protein in an ionic liquid is provided. The protein solution of the present invention is obtained by dissolving a poorly soluble protein in water. Poorly soluble proteins are inherently difficult to dissolve in water, and therefore, even using gel permeation chromatography, they remain insoluble in water, making molecular weight determination impossible or impractical. Furthermore, it is difficult to decompose the protein solution (obtained by dissolving a poorly soluble protein in an ionic liquid) and determine its amino acid sequence.

[0112] In one embodiment of the present invention, the protein solution of the water-insoluble protein of the present invention can be a protein solution obtained without causing hydrolysis or other formation of protein derivatives in the water-insoluble protein. In other embodiments, the protein solution of the water-insoluble protein of the present invention can be a protein solution obtained by partially hydrolyzing the water-insoluble protein and dissolving the resulting hydrolysate in an ionic liquid. In other embodiments, the protein solution of the water-insoluble protein of the present invention can be a protein solution obtained by partially reducing the water-insoluble protein and dissolving the resulting reduced product in an ionic liquid. In one embodiment of the present invention, the protein solution of the water-insoluble protein of the present invention can be obtained by dissolving the water-insoluble protein in an ionic liquid. Substances described in other parts of this specification can be used as the ionic liquid and / or its concentration, and as the water-insoluble protein.

[0113] [Regeneration of soluble, water-insoluble proteins] One aspect of the present invention provides a method for manufacturing a polymer molded article containing a water-insoluble protein, the method comprising: (a) a step of providing an ionic liquid in which the water-insoluble protein and a hydroxyl-containing polymer are dissolved; and (b) a step of precipitating the polymer molded article from the ionic liquid.

[0114] One aspect of the present invention provides a method for manufacturing a polymer molded article containing a water-insoluble protein, the method comprising: (a) a step of mixing the water-insoluble protein with an ionic liquid; (b) a step of mixing a hydroxyl-containing polymer with an ionic liquid; (c) a step of mixing the protein solution obtained in step (a) with the polymer solution obtained in step (b); and (d) a step of precipitating the polymer molded article from the solution obtained in step (c).

[0115] One aspect of the present invention provides a method for manufacturing a polymer molded body containing a water-insoluble protein, the method comprising: (a') a step of mixing the water-insoluble protein and a hydroxyl-containing polymer into an ionic liquid in a single step or sequentially; and (b') a step of precipitating the polymer molded body from the solution obtained in step (a').

[0116] In one embodiment of the invention, water-insoluble proteins and hydroxyl-containing polymers dissolved in an ionic solution can be precipitated and regenerated by contacting them with a poor solvent containing these solutes. In other embodiments, an ionic liquid containing dissolved water-insoluble proteins and hydroxyl-containing polymers is coated in a thin film onto a glass plate or similar object, which is then immersed in a poor solvent containing these solutes, precipitating out as a thin film, thereby regenerating the mixture of water-insoluble proteins and hydroxyl-containing polymers. In one embodiment, the regenerated form can be cotton-like or film-like, and after regeneration, it can be further washed and dried to produce a final product.

[0117] In this specification, "poor solvent" for water-insoluble proteins and hydroxyl-containing polymers refers to a solvent in which at most about 1% of the water-insoluble proteins and hydroxyl-containing polymers dissolve at room temperature. Examples of poor solvents for water-insoluble proteins and hydroxyl-containing polymers include water; lower alcohols; and mixed solvents of ionic liquids with water and / or lower alcohols. From the viewpoint of recycling and reusing ionic liquids, mixed solvents of ionic liquids with water and / or lower alcohols are preferred as poor solvents.

[0118] In one embodiment of the present invention, for water-insoluble proteins and hydroxyl-containing polymers dissolved in ionic liquids, the mixture of water-insoluble proteins and hydroxyl-containing polymers can be precipitated and regenerated by contacting it with a poor solvent containing an acidic substance, thereby improving the residual rate of the water-insoluble proteins. The acidic substance is not particularly limited; in one embodiment, examples of acidic substances include organic acids such as acetic acid and citric acid, and inorganic acids such as hydrochloric acid, phosphoric acid, and sulfuric acid. The acid concentration of the acidic substance is not particularly limited; from the viewpoint of improving the residual rate of water-insoluble proteins and the recycling of ionic liquids, a solution of about 0.1% to about 20% by weight is preferred.

[0119] In one aspect of the invention, a polymer molded article containing a water-insoluble protein is provided, the molded article being obtained by a method comprising the steps of: (a) providing an ionic liquid in which the water-insoluble protein and a hydroxyl-containing polymer are dissolved; and (b) precipitating the polymer molded article from the ionic liquid.

[0120] In one aspect of the invention, a polymer molded article containing a water-insoluble protein is provided, the molded article being prepared by a method comprising the steps of: (a) mixing the water-insoluble protein with an ionic liquid; (b) mixing a hydroxyl-containing polymer with an ionic liquid; (c) mixing the protein solution obtained in step (a) with the polymer solution obtained in step (b); and (d) precipitating the polymer molded article from the solution obtained in step (c).

[0121] In one aspect of the invention, a polymer molded article containing a water-insoluble protein is provided, the molded article being obtained by a method comprising the steps of: (a') mixing the water-insoluble protein and the hydroxyl-containing polymer in an ionic liquid in a single step or sequentially; and (b') precipitating the polymer molded article from the solution obtained by step (a').

[0122] As described above, the protein solution of the present invention is obtained by dissolving a water-insoluble protein. Water-insoluble proteins are inherently difficult to dissolve in water, and therefore, even using gel permeation chromatography, they remain insoluble in water, making molecular weight determination impossible or impractical. Furthermore, it is difficult to decompose the protein solution (obtained by dissolving a water-insoluble protein in an ionic liquid) to determine its amino acid sequence. Therefore, it is also difficult to characterize the polymer molded articles obtained from such protein solutions of the present invention using structural and / or property-specific properties.

[0123] In one embodiment, there are no particular limitations on the polymer molded body; any molded body obtained from a hydroxyl-containing polymer that is the material used in the method of the present invention is acceptable, such as fibers, nonwoven fabrics, films, etc. Characteristics of the molded body obtained by the method of the present invention include moisture absorption and release, deodorization, antibacterial properties, skin protection, cell proliferation, biocompatibility, and metal adsorption.

[0124] In one embodiment, in the method for preparing the polymer molded article of the present invention, the ionic liquid used as a solvent for the water-insoluble protein and the ionic liquid used as a solvent for the hydroxyl-containing polymer can be the same or different. In one embodiment, 1-ethyl-3-methylimidazolium acetate can be used as an ionic liquid, for example. For reuse of the ionic liquid, the solvents used to dissolve the water-insoluble protein and the hydroxyl-containing polymer are preferably the same.

[0125] In one embodiment, the aqueous solution of the ionic liquid used for the precipitation of water-insoluble proteins can be concentrated and reused in the method of the present invention.

[0126] In one embodiment, a modified hydroxyl-containing polymer with functional groups bonded to it can also be used as the hydroxyl-containing polymer. By mixing an ionic liquid containing a water-insoluble protein and an ionic liquid containing the modified hydroxyl-containing polymer, and extruding the mixture into water and / or a poor solvent containing the solute, a functionalized polymer molded article can be obtained.

[0127] In one embodiment, as a hydroxyl-containing polymer or a modified hydroxyl-containing polymer, a saponification of cellulose, regenerated cellulose, polyvinyl alcohol, and polyvinyl acetate can be used, with regenerated cellulose being preferred. Furthermore, the polyvinyl acetate saponification need only contain hydroxyl (OH) groups as long as a portion of the polyvinyl acetate is also saponified.

[0128] In one embodiment, the protein solution and its molded form of the water-insoluble protein dissolved by the method of the present invention have the functions and / or properties of the water-insoluble protein itself as the material. For example, in addition to moisturizing, metal ion adsorption, deodorizing, and antibacterial effects, it may also have physiological activities such as fibroblast proliferation, active enzyme removal, and cell activation.

[0129] In one embodiment, a polymer material may be selected to leverage the functions and / or properties of water-insoluble proteins. In one embodiment of the invention, the protein solution and / or polymer molded body are derived from water-insoluble proteins such as eggshell membranes from natural sources, thus exhibiting excellent adhesion to human skin tissue. Furthermore, in one embodiment of the invention, since the protein solution and / or polymer molded body contain components of water-insoluble proteins such as eggshell membranes, they can possess properties substantially similar to those of natural water-insoluble proteins such as eggshell membranes.

[0130] [Reagent test kit] In one aspect of the invention, a kit is provided for preparing polymer molded articles containing water-insoluble proteins, the kit comprising an ionic liquid, a hydroxyl-containing polymer, and a water-insoluble protein.

[0131] By using such a kit, solutions obtained by dissolving water-insoluble proteins in ionic liquids, and / or polymeric articles containing water-insoluble proteins, can be readily obtained. In one embodiment, the water-insoluble proteins, ionic liquids, and hydroxyl-containing polymers in the kit of the present invention may be those described in other parts of this specification.

[0132] In this specification, "or" is used when "at least one or more" of the items listed in the text can be used. The same applies to "or". In cases where it is explicitly stated in this specification as a "range" of "two values", the range also includes the two values ​​themselves.

[0133] All scientific literature, patents, patent applications, and other references cited in this specification are cited here as a whole to the extent that they are specifically described therein.

[0134] For ease of understanding, preferred embodiments have been shown to illustrate this disclosure. The following description is based on embodiments; however, the above description and the following embodiments are for illustrative purposes only and are not intended to limit the scope of this disclosure. Therefore, the scope of this disclosure is not limited to the embodiments and examples specifically described in this specification, but is limited to the claims.

[0135] Example (Fabrication of polymer molded parts) According to Table 1 below, water-insoluble proteins (eggshell membrane, wool, chicken feathers, hydrolyzed eggshell membrane (water-insoluble) and hydrolyzed eggshell membrane (water-soluble)), cellulose, and surfactants were dissolved in ionic liquids, respectively. Protein concentrations and / or quantities are shown in Table 1. In the table, the units for ionic liquids, surfactants, proteins, cellulose, etc., are by weight (%).

[0136] [Table 1]

[0137] The symbols in Table 1 are shown in Table 2 below.

[0138] [Table 2]

[0139] In Table 1, "hydrolyzed eggshell membrane (water insoluble)" refers to eggshell membranes obtained by partial hydrolysis, while "hydrolyzed eggshell membrane (water soluble)" refers to eggshell membranes hydrolyzed to the point of being soluble in water. The conditions for each hydrolysis are as follows.

[0140] Hydrolyzed eggshell membrane (water-insoluble) refers to the substance obtained by hydrolyzing the eggshell membrane in an approximately 5% alkaline aqueous solution at approximately 60°C for about 1 hour, neutralizing it with hydrochloric acid of the same alkaline equivalent, washing the precipitated hydrolysate with water, and drying it. Conversely, hydrolyzed eggshell membrane (water-soluble) refers to the substance obtained by hydrolyzing the eggshell membrane in an approximately 5% alkaline aqueous solution at approximately 60°C for about 1 hour, neutralizing it with hydrochloric acid of the same alkaline equivalent, filtering the precipitate, and leaving the hydrolysate remaining in the filtrate.

[0141] For water-insoluble proteins and cellulose, the proteins are dissolved in the ionic liquids shown in Table 1, and cellulose is added to the solution to continue dissolving, thus preparing a mixed solution.

[0142] Next, the doped solutions obtained in the various examples and comparative examples shown in Table 1 were heated to 50°C to 60°C and spun by extruding them into water.

[0143] The evaluation symbols for spinnability are as follows.

[0144] ◎: Good spinning properties 〇: It can be spun, but it has high viscosity and requires special handling such as high-temperature spinning. ×: Cannot be spun into powder, etc. Then, the obtained spun cotton was washed and dried, and the measured nitrogen content was analyzed by the Kjeldahl method. The nitrogen content was then multiplied by the protein conversion factor to obtain the measured protein content. The spun cotton was then dried in a circulating dryer (105℃, 90 minutes) and converted to solids content to calculate the measured protein content / solids content.

[0145] The ratio of measured protein content / solid content to theoretical protein content / solid content is calculated as the residual rate.

[0146] As shown in Table 1, a retention rate of over 80% was confirmed in Examples 1 to 11. In Comparative Example 2, which used hydrolyzed eggshell membrane (water-soluble), the retention rate was 12%. Furthermore, the polymer molded body prepared according to Comparative Example 1 (which did not contain cellulose) turned into powder, making spinning impossible and thus protein content determination was not performed.

[0147] Spinning was performed using the solutions obtained in Examples 2, 11, and Comparative Example 2. Electron micrographs (SEM) of the cross-sections of the resulting spun cotton fabrics are shown below. Figures 1-3 In the spun cotton of Example 2, it is known that the fibers are oriented longitudinally relative to the cross-section ( Figure 1 Even in Example 11, where no surfactant was added, spinning was possible despite the fibers not being oriented relative to the cross section. Figure 2 In Comparative Example 2, although spinning was possible, from the perspective of effectively utilizing water-insoluble proteins, a residue rate of 12% was deemed unsuitable for efficient utilization. Figure 3 ).

[0148] (Note) As described above, this disclosure has been illustrated using preferred embodiments; however, it should be understood that the scope of this disclosure should be interpreted solely by the claims. It should be understood that all patents, patent applications, and other documents referenced in this specification are incorporated herein by reference as if their contents were specifically stated herein. This application claims priority to Japanese Patent Application No. 2023-178243, filed October 16, 2023, by the Japanese Patent Office, the contents of which are incorporated herein by reference in such a manner that they constitute the content of this application.

[0149] Industrial availability The protein solution and its molded form obtained by the method of the present invention can be used in chemical applications in the form of fibers and films, in face mask materials and / or in powder form for cosmetic applications, in medical applications as wound dressing materials, and in the regenerative medicine field as cell culture scaffolding materials. Furthermore, due to its good biodegradability, it is also expected to be used as an agricultural material.

Claims

1. A method for dissolving water-insoluble proteins, wherein, This includes the process of mixing hydroxyl-containing polymers, water-insoluble proteins, and ionic liquids.

2. The method according to claim 1, wherein, The process further includes the step of mixing a co-solvent.

3. The method according to claim 2, wherein, The cosolvent is a surfactant.

4. The method according to claim 3, wherein, The surfactant is a cationic surfactant with a superacid as its opposite ion.

5. The method according to claim 3, wherein, When the total weight of the water-insoluble protein and the hydroxyl-containing polymer is 100% by weight, the content of the surfactant is about 0.05% by weight to about 10% by weight.

6. The method according to claim 1, wherein, When the total weight of the water-insoluble protein and the hydroxyl-containing polymer is 100% by weight, the content of the water-insoluble protein is about 5% by weight to about 90% by weight.

7. The method according to claim 1, wherein, The water-insoluble proteins include eggshell membranes, wool, chicken feathers, silk, and scales, as well as their partially hydrolyzed products and / or partially reduced products.

8. The method according to claim 1, wherein, The ionic liquid contains imidazolium cations, pyridinium cations, and alkylammonium cations.

9. The method according to claim 1, wherein, The hydroxyl-containing polymer includes a modified hydroxyl-containing polymer to which functional functional groups are incorporated.

10. The method according to claim 1, wherein, The hydroxyl-containing polymers include saponifications of cellulose, polyvinyl alcohol, and polyvinyl acetate.

11. A solution obtained by dissolving a hydroxyl-containing polymer and a water-insoluble protein in an ionic liquid.

12. The solution according to claim 11, wherein, It further contains a co-solvent.

13. The solution according to claim 12, wherein, The cosolvent is a surfactant.

14. The solution according to claim 13, wherein, The surfactant is a cationic surfactant with a superacid as its opposite ion.

15. The solution according to claim 13, wherein, When the total weight of the water-insoluble protein and the hydroxyl-containing polymer is 100% by weight, the content of the surfactant is about 0.05% by weight to about 10% by weight.

16. The solution according to claim 11, wherein, When the total weight of the water-insoluble protein and the hydroxyl-containing polymer is 100% by weight, the content of the water-insoluble protein is about 5% by weight to about 90% by weight.

17. The solution according to claim 11, wherein, The water-insoluble proteins include eggshell membranes, wool, chicken feathers, silk, and scales, as well as their partially hydrolyzed products and / or partially reduced products.

18. The solution according to claim 11, wherein, The ionic liquid contains imidazolium cations, pyridinium cations, and alkylammonium cations.

19. The solution according to claim 11, wherein, The hydroxyl-containing polymer includes a modified hydroxyl-containing polymer to which functional functional groups are incorporated.

20. The solution according to claim 11, wherein, The hydroxyl-containing polymers include saponifications of cellulose, polyvinyl alcohol, and polyvinyl acetate.

21. A method for manufacturing a polymer molded article containing a water-insoluble protein, wherein, include: A process for dissolving ionic liquids containing poorly soluble proteins and hydroxyl polymers; as well as The process of precipitating polymer molded articles from the ionic liquid.

22. The method according to claim 21, wherein, The ionic liquid is provided through the following process: (a) The process of mixing water-insoluble proteins with ionic liquids; (b) The process of mixing a hydroxyl-containing polymer with an ionic liquid; and (c) The process of mixing the protein solution obtained from step (a) with the polymer solution obtained from step (b).

23. The method according to claim 21, wherein, The ionic liquid is provided through the following process: A process of mixing water-insoluble proteins and hydroxyl-containing polymers into an ionic liquid in one step or sequentially.

24. The method according to claim 21, wherein, A co-solvent is further mixed into the ionic liquid.

25. The method according to claim 21, wherein, The water-insoluble proteins include eggshell membranes, wool, chicken feathers, silk, and scales, as well as their partially hydrolyzed products and / or partially reduced products.

26. The method according to claim 21, wherein, The ionic liquid contains imidazolium cations, pyridinium cations, and alkylammonium cations.

27. The method according to claim 21, wherein, The hydroxyl-containing polymer includes a modified hydroxyl-containing polymer to which functional functional groups are incorporated.

28. The method according to claim 21, wherein, The hydroxyl-containing polymers include saponifications of cellulose, polyvinyl alcohol, and polyvinyl acetate.

29. A method for manufacturing a polymer molded article containing a water-insoluble protein, wherein, The process includes the step of extruding the solution of claim 11 into a poor solvent containing a hydroxyl polymer and a water-insoluble protein.

30. A polymer molded article containing a water-insoluble protein, wherein, This molded body is obtained through a method including the following steps: A process for dissolving ionic liquids containing poorly soluble water-soluble proteins and hydroxyl-containing polymers; and The process of precipitating polymer molded articles from the ionic liquid.

31. The molded article according to claim 30, wherein, The ionic liquid is provided through the following process: (a) The process of mixing water-insoluble proteins with ionic liquids; (b) The process of mixing a hydroxyl-containing polymer with an ionic liquid; and (c) The process of mixing the protein solution obtained from step (a) with the polymer solution obtained from step (b).

32. The molded article according to claim 30, wherein, The ionic liquid is provided through the following process: A process of mixing water-insoluble proteins and hydroxyl-containing polymers into an ionic liquid in one step or sequentially.

33. The molded article according to claim 30, wherein, The molded body includes fibers, yarns, fabrics, knitted fabrics, woven fabrics, nonwoven fabrics, and films.

34. A kit for manufacturing polymer molded articles containing water-insoluble proteins, wherein, It includes ionic liquids, hydroxyl-containing polymers, and water-insoluble proteins.

35. The kit according to claim 34, wherein, It further contains a co-solvent.

36. The kit according to claim 35, wherein, The cosolvent is a surfactant.

37. The kit according to claim 36, wherein, The surfactant is a cationic surfactant with a superacid as its opposite ion.

38. The kit according to claim 36, wherein, When the total weight of the water-insoluble protein and the hydroxyl-containing polymer is 100% by weight, the content of the surfactant is about 0.05% by weight to about 10% by weight.

39. The kit according to claim 34, wherein, When the total weight of the water-insoluble protein and the hydroxyl-containing polymer is 100% by weight, the content of the water-insoluble protein is about 5% by weight to about 90% by weight.

40. The kit according to claim 34, wherein, The water-insoluble proteins include eggshell membranes, wool, chicken feathers, silk, and scales, as well as their partially hydrolyzed products and / or partially reduced products.

41. The kit according to claim 34, wherein, The ionic liquid contains imidazolium cations, pyridinium cations, and alkylammonium cations.

42. The kit according to claim 34, wherein, The hydroxyl-containing polymer includes a modified hydroxyl-containing polymer to which functional functional groups are incorporated.

43. The kit according to claim 34, wherein, The hydroxyl-containing polymers include saponifications of cellulose, polyvinyl alcohol, and polyvinyl acetate.

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