Technological method for extracting basswood ganoderma spore essential oil under assistance of ultrasonic enzyme

Abstract

The invention discloses a process method for ultrasonic enzyme-assisted extraction of basswood ganoderma spore essential oil, which comprises the following steps: mixing basswood ganoderma spores, a buffer solution and a compound enzyme, and carrying out water bath treatment to obtain an enzymolysis sample; adding an organic solvent into the enzymolysis sample, and carrying out ultrasonic treatment to obtain an extracting solution; carrying out boiling water bath treatment on the extracting solution, and then carrying out centrifugal separation to obtain supernate containing essential oil; performing rotary evaporation distillation on the supernatant containing the essential oil, and then drying and removing impurities to obtain the basswood ganoderma spore essential oil. Through the synergistic effect of compound enzyme enzymolysis and ultrasonic extraction, efficient wall breaking and grease dissolution are achieved for the cell wall structure of the basswood ganoderma lucidum spores more compact than bag ganoderma lucidum spores, and the extraction rate of the basswood ganoderma lucidum spore essential oil is remarkably increased; the problems of low efficiency, easy loss of active components, insufficient product purity and the like in the traditional extraction technology are solved.

Description

Technical Field

[0001] This invention relates to the field of food technology, and in particular to a process for ultrasonic enzyme-assisted extraction of essential oil from Ganoderma lucidum spores on linden wood. Background Technology

[0002] Linden wood Ganoderma lucidum spore oil, as the core active extract of linden wood Ganoderma lucidum spores, is rich in bioactive components such as triterpenoids, unsaturated fatty acids, and ergosterol. It has significant effects in immune regulation, anti-oxidation, and anti-inflammation, and is widely used in the fields of medicine, health products, and cosmetics. Market demand continues to grow.

[0003] Because the cell walls of Ganoderma lucidum spores grown on logs are denser than those grown in bags, the essential oil from the spores is difficult to release. Traditional methods for extracting Ganoderma lucidum spore oil have many limitations. For example, the extraction rate of single solvent extraction is low and time-consuming, while purely physical-assisted extraction methods are prone to degradation of active ingredients. In addition, current methods that extract Ganoderma lucidum spore oil through solvent extraction, physical assistance, or combined technologies still suffer from problems such as suboptimal extraction rates, insufficient matching of process parameters, high equipment costs, or the need to improve product purity.

[0004] Therefore, developing a highly efficient, high-purity, and stable Ganoderma lucidum spore oil extraction technology is of great value. Summary of the Invention

[0005] In view of this, this application provides a process for ultrasonic enzyme-assisted extraction of essential oil from Ganoderma lucidum spores on linden wood, which is used to solve the problem of how to improve the extraction rate of essential oil from Ganoderma lucidum spores on linden wood.

[0006] To achieve the above technical objectives, this application adopts the following technical solution: A process for ultrasonic enzyme-assisted extraction of essential oil from Ganoderma lucidum spores from linden wood, characterized by comprising the following steps: Linden wood Ganoderma lucidum spores, buffer solution and compound enzyme were mixed and subjected to water bath treatment to obtain enzymatic hydrolysis samples; An organic solvent was added to the enzymatically hydrolyzed sample, and the sample was subjected to ultrasonic treatment to obtain an extract. The extract was subjected to a boiling water bath and then centrifuged to obtain a supernatant containing essential oil. The supernatant containing the essential oil is subjected to rotary evaporation distillation, followed by drying to remove impurities, to obtain the linden wood Ganoderma lucidum spore essential oil.

[0007] Preferably, the buffer solution is a disodium hydrogen phosphate-citric acid buffer solution.

[0008] Preferably, the pH value of the buffer solution is 5-6.

[0009] Preferably, the complex enzyme is a mixture of papain and cellulase.

[0010] Preferably, the mass ratio of papain to cellulase is 1:1-1.2.

[0011] Preferably, the total amount of the compound enzyme added is 1-2% of the mass of the Ganoderma lucidum spores.

[0012] Preferably, the water bath temperature is 50-60℃ and the water bath time is 1 hour.

[0013] Preferably, the organic solvent is n-hexane.

[0014] Preferably, the material-to-liquid ratio for ultrasonic treatment is 1:10-30, the ultrasonic treatment time is 20-30 min, and the ultrasonic power is 200-400 W.

[0015] Preferably, the drying and impurity removal method is as follows: first absorb water with anhydrous Na2SO4, and then remove impurities by centrifugation.

[0016] The beneficial effects of this application are as follows: This application achieves efficient cell wall breaking and oil dissolution by combining the synergistic effect of compound enzyme hydrolysis and ultrasonic extraction, targeting the denser cell wall structure of Ganoderma lucidum spores from linden wood compared to those from bagged materials. This significantly improves the extraction rate of essential oil from linden wood Ganoderma lucidum spores and solves the problems of low efficiency, easy loss of active ingredients, and insufficient product purity in traditional extraction techniques. Detailed Implementation

[0017] The embodiments of the technical solution of this application will be described in detail below. The following embodiments are only used to illustrate the technical solution of this application more clearly, and are therefore only examples, and should not be used to limit the scope of protection of this application.

[0018] In the description of the embodiments of this application, technical terms such as "first" and "second" are used only to distinguish different objects and should not be construed as indicating or implying relative importance or implicitly specifying the number, specific order, or primary and secondary relationship of the indicated technical features. In the description of the embodiments of this application, "multiple" means two or more, unless otherwise explicitly defined.

[0019] In this document, the term "embodiment" means that a particular feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of this phrase in various places throughout the specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment mutually exclusive with other embodiments. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0020] In the description of the embodiments in this application, the term "and / or" is merely a description of the relationship between related objects, indicating that three relationships can exist. For example, A and / or B can represent: A existing alone, A and B existing simultaneously, and B existing alone. Additionally, the character " / " in this document generally indicates that the preceding and following related objects have an "or" relationship.

[0021] Unless otherwise explicitly defined and specified herein, all technical and scientific terms used in this application shall have the generally accepted meanings understood by one of ordinary skill in the field of chemical and chemical materials technology (including but not limited to polymer chemistry, inorganic chemistry, organic synthesis, catalysis chemistry, materials processing, and chemical unit operations) based on their professional knowledge and conventional practice. The use of any terminology herein is intended to describe the specific embodiments of this application in the clearest and most accurate manner, so as to fully disclose the technical solution. Such use shall not in any way be construed as a limitation on the scope of the claims, nor does it imply the exclusion of equivalent technical solutions that could be reasonably known by one of skill in the art based on the concept of this application.

[0022] The terms "comprising," "including," "having," "containing," and any grammatical variations or similar expressions used in the specification and claims of this application are all open-ended and non-exhaustive descriptive terms. Their purpose is to explicitly describe the existence of the stated technical features, components, steps, or parts, while explicitly allowing and covering the possibility that other features, components, steps, parts, or any combinations thereof not explicitly listed may exist or be added to the technical solution, as long as such additions do not destroy the integrity and inventiveness of the original technical solution.

[0023] When the terms "embodiments," "some embodiments," or "specific embodiments" are mentioned in the specification, they refer to examples that, in conjunction with the specific parameters, materials, steps, and results described in that section, constitute one or a group of examples for implementing the technical solutions of this application. These embodiments are used for full disclosure and illustrative purposes, not for exhaustive enumeration. Those skilled in the art should understand that, without departing from the overall inventive concept of this application, the various technical features disclosed in different embodiments can be combined, substituted, modified, or deleted to form other implementation methods that are not listed one by one in the specification but also fall within the protection scope of this application.

[0024] Unless otherwise expressly specified and limited, all terms related to chemical process operations, material preparation, processing and analytical testing involved in this application shall be interpreted in the broadest sense based on the conventional understanding of those skilled in the art.

[0025] Regarding performance testing and structural characterization, all testing and characterization methods involved in this application, unless otherwise specified, refer to conventional methods known in the art. Specific testing conditions may be selected and adjusted according to the sample properties and relevant national standards, international standards, or industry-standard methods. Test items may include mechanical properties (such as tensile, bending, and impact strength), thermal properties (such as DSC and TGA analysis), and chemical stability (such as solvent resistance and acid / alkali corrosion resistance). Structural characterization methods may include FT-IR, NMR, XRD, SEM, TEM, and BET. All test results should be understood to be within the allowable range of conventional experimental errors.

[0026] Regarding numerical values ​​and ranges, all parameter ranges expressed in this application in the form of "from a certain value to a certain value" should be understood as explicitly disclosing the endpoints of the range, each specific numerical point between the endpoints, and all sub-ranges formed by any two numerical points within the range. For example, "30℃ to 80℃" discloses 30, 31, ..., 80℃, as well as sub-ranges such as 30-50℃, 45-70℃, etc. When a numerical value is preceded by "about," "approximately," or similar words, it indicates that the numerical value is allowed to have reasonable errors recognized in the art under the measurement or control conditions, which can generally be understood as the deviation allowed by relevant standards or a normal fluctuation range of ±5% or ±10%.

[0027] A process for ultrasonic enzyme-assisted extraction of essential oil from Ganoderma lucidum spores from linden wood, characterized by comprising the following steps: Linden wood Ganoderma lucidum spores, buffer solution and compound enzyme were mixed and subjected to water bath treatment to obtain enzymatic hydrolysis samples; An organic solvent was added to the enzymatically hydrolyzed sample, and the sample was subjected to ultrasonic treatment to obtain an extract. The extract was subjected to a boiling water bath and then centrifuged to obtain a supernatant containing essential oil. The supernatant containing the essential oil is subjected to rotary evaporation distillation, followed by drying to remove impurities, to obtain the linden wood Ganoderma lucidum spore essential oil.

[0028] In this application, the water bath treatment is a constant-temperature enzymatic hydrolysis process. Its function is to utilize the synergistic catalytic activity of the compound enzyme to target and degrade the dense cell wall structure of Ganoderma lucidum spores, thereby breaking down the barrier formed by cellulose and protein components, thus removing the barrier to oil dissolution and creating conditions for subsequent extraction. In this application, the compound enzyme first degrades the cellulose and protein components in the dense cell wall of Ganoderma lucidum spores, opening the oil dissolution channel. Subsequently, ultrasound accelerates the diffusion of oil into the organic solvent through the cavitation effect, improving the extraction rate and overcoming the defect of low extraction rate of single solvent method. Through a multi-stage purification process of boiling water bath inactivation, centrifugation, rotary evaporation distillation and drying to remove impurities, enzyme residues, solvents, moisture and flocculent impurities are effectively removed, ensuring high purity of the product.

[0029] In some embodiments, the buffer solution is disodium hydrogen phosphate-citrate buffer.

[0030] In this embodiment, the buffer solution can stably maintain the pH environment of the enzymatic hydrolysis reaction system and avoid the decrease in the activity of the complex enzyme due to pH fluctuations.

[0031] In some embodiments, the pH of the buffer solution is 5-6.

[0032] In this embodiment, the pH range is close to the optimal reaction pH of papain and cellulase, which is conducive to both enzymes maintaining high catalytic activity at the same time, and achieving efficient synergistic degradation of cell wall components.

[0033] In some embodiments, the complex enzyme is a mixture of papain and cellulase.

[0034] In this embodiment, cellulase is responsible for degrading the cellulose skeleton in the cell wall, while papain hydrolyzes cell wall proteins and lipid-bound protein components. The two work synergistically to enhance cell wall permeability.

[0035] In some embodiments, the mass ratio of papain to cellulase is 1:1-1.2.

[0036] In this embodiment, the ratio can balance the degradation efficiency of the two enzymes and avoid substrate competition or reaction imbalance caused by excessive use of a single enzyme.

[0037] In some embodiments, the total amount of the compound enzyme added is 1-2% of the mass of the Ganoderma lucidum spores.

[0038] In this embodiment, the amount added ensures sufficient enzyme activity to complete cell wall degradation while avoiding increased costs and subsequent inactivation burden caused by excessive enzyme.

[0039] In some embodiments, the water bath treatment temperature is 50-60°C and the water bath time is 1 hour.

[0040] In this embodiment, the temperature range can maintain the thermal stability and catalytic activity of the complex enzyme while also softening the cell wall structure to a moderate degree; the 1-hour enzymatic hydrolysis time is sufficient to complete the degradation of the main cell wall components, while avoiding the loss of active ingredients caused by long-term treatment.

[0041] In some embodiments, the organic solvent is n-hexane.

[0042] In this embodiment, n-hexane exhibits excellent solubility and selectivity for non-polar active components (such as triterpenoids and unsaturated fatty acids) in Ganoderma lucidum spore oil, and has a low boiling point and is easy to recover.

[0043] In some embodiments, the material-to-liquid ratio of the ultrasonic treatment is 1:10-30, the ultrasonic treatment time is 20-30 min, and the ultrasonic power is 200-400 W.

[0044] In this embodiment, the parameter range accelerates the penetration of organic solvents into the interior of the broken spores through the cavitation effect and mechanical vibration of ultrasound, promoting the rapid diffusion of essential oils from the solid phase to the solvent phase, thus forming a synergistic effect with enzymatic hydrolysis.

[0045] In some embodiments, the drying and impurity removal method is as follows: first absorb water with anhydrous Na2SO4, and then remove impurities by centrifugation.

[0046] In this embodiment, anhydrous Na2SO4 can effectively adsorb residual moisture in crude essential oil, preventing moisture from affecting the purity of the essential oil; subsequent high-speed centrifugation can remove flocculent impurities precipitated after moisture adsorption, thereby obtaining high-purity, low-moisture-content Ganoderma lucidum spore essential oil.

[0047] The following specific embodiments further illustrate this solution.

[0048] Example 1 A process for ultrasonic enzyme-assisted extraction of essential oil from Ganoderma lucidum spores from linden wood includes the following steps: Select Ganoderma lucidum spores from linden wood that are free of impurities and mold. Weigh 10.0g accurately using an analytical balance and place them in a 500mL Erlenmeyer flask with a stopper. Add 300mL of pH 5.4 disodium hydrogen phosphate-citric acid buffer solution to the flask at a material-to-liquid ratio of 1:30 (g:mL). Then add 0.14g of a compound enzyme (papain and cellulase mixed in a 1:1 mass ratio) (accounting for 1.40% of the spore mass). After sealing, place the flask in a 60℃ constant temperature water bath and enzymatically hydrolyze for 1h to fully degrade the residual cell wall components and obtain the enzymatically hydrolyzed sample. Transfer the enzymatically hydrolyzed sample to a fume hood, slowly add 100 mL of n-hexane, stir evenly with a glass rod until the mixture is homogeneous, tighten the stopper to prevent solvent evaporation, fix the Erlenmeyer flask in an ultrasonic cleaner, set the ultrasonic power to 200 W, ultrasonic time to 30 min, ultrasonic temperature to room temperature, start the equipment for ultrasonic extraction, and obtain the extract. After sonication, the flask was immediately placed in a boiling water bath for 5 minutes to rapidly inactivate the complex enzyme and terminate the enzymatic hydrolysis reaction. Then, it was taken out and cooled to room temperature. The cooled mixture was transferred to a centrifuge tube and placed in a centrifuge. It was centrifuged at 4500 r / min for 5 minutes to fully separate the solid and liquid. The supernatant containing essential oil was collected by pipette. The collected supernatant was transferred to a distillation flask in a rotary evaporator. The distillation temperature was set to 50℃ and the vacuum degree to -0.09MPa. The equipment was started for rotary evaporation to recover the n-hexane solvent until no solvent dripped from the distillation flask, thus obtaining crude Ganoderma lucidum spore essential oil. 5% anhydrous Na2SO4 (based on the mass of crude oil) was added to the crude essential oil, and after shaking and mixing, it was allowed to stand for 20 hours to fully absorb water. Then, the sample was transferred to a small centrifuge tube and centrifuged at 7000 r / min for 5 min to remove flocculent impurities and anhydrous Na2SO4 residue. The clear oily liquid at the top after centrifugation was collected, which is the Ganoderma lucidum spore essential oil. The extraction rate was measured to be 8.1%.

[0049] Example 2 A process for ultrasonic enzyme-assisted extraction of essential oil from Ganoderma lucidum spores from linden wood includes the following steps: Select Ganoderma lucidum spores from linden wood that are free of impurities and mold. Weigh 10.0g accurately using an analytical balance and place them in a 500mL Erlenmeyer flask with a stopper. Add 100mL of pH 5.4 disodium hydrogen phosphate-citric acid buffer solution to the flask at a material-to-liquid ratio of 1:30 (g:mL). Then add 0.14g of a compound enzyme (papain and cellulase mixed in a 1:1 mass ratio) (accounting for 1.40% of the spore mass). After sealing, place the flask in a 60℃ constant temperature water bath and enzymatically hydrolyze for 1h to fully degrade the residual cell wall components and obtain the enzymatically hydrolyzed sample. Transfer the enzymatically hydrolyzed sample to a fume hood, slowly add 100 mL of n-hexane, stir evenly with a glass rod until the mixture is homogeneous, tighten the stopper to prevent solvent evaporation, fix the Erlenmeyer flask in an ultrasonic cleaner, set the ultrasonic power to 300 W, ultrasonic time to 20 min, and ultrasonic temperature to room temperature, start the equipment for ultrasonic extraction, and obtain the extract. After sonication, the flask was immediately placed in a boiling water bath for 5 minutes to rapidly inactivate the complex enzyme and terminate the enzymatic hydrolysis reaction. Then, it was taken out and cooled to room temperature. The cooled mixture was transferred to a centrifuge tube and placed in a centrifuge. It was centrifuged at 4500 r / min for 5 minutes to fully separate the solid and liquid. The supernatant containing essential oil was collected by pipette. The supernatant containing the essential oil was transferred to a distillation flask in a rotary evaporator. The distillation temperature was set to 50℃ and the vacuum degree to -0.09MPa. The equipment was started for rotary evaporation to recover the n-hexane solvent until no solvent dripped from the distillation flask, thus obtaining crude Ganoderma lucidum spore essential oil. 5% anhydrous Na2SO4 (based on the mass of crude oil) was added to the crude essential oil, and after shaking and mixing, it was allowed to stand for 20 hours to fully absorb water. Then, the sample was transferred to a small centrifuge tube and centrifuged at 7000 r / min for 5 min to remove flocculent impurities and anhydrous Na2SO4 residue. The clear oily liquid at the top after centrifugation was collected, which is the Ganoderma lucidum spore essential oil. The extraction rate was measured to be 7.3%.

[0050] Example 3 A process for ultrasonic enzyme-assisted extraction of essential oil from Ganoderma lucidum spores from linden wood includes the following steps: Select Ganoderma lucidum spores from linden wood that are free of impurities and mold. Weigh 10.0g accurately using an analytical balance and place them in a 500mL Erlenmeyer flask with a stopper. Add 200mL of pH 5.4 disodium hydrogen phosphate-citric acid buffer solution to the flask at a material-to-liquid ratio of 1:20 (g:mL). Then add 0.14g of a compound enzyme (papain and cellulase mixed in a 1:1 mass ratio) (accounting for 1.40% of the spore mass). After sealing, place the flask in a 60℃ constant temperature water bath and perform enzymatic hydrolysis with constant temperature shaking for 1h to fully degrade the residual cell wall components and obtain the enzymatic hydrolysate sample. Transfer the enzymatically hydrolyzed sample to a fume hood, slowly add 100 mL of n-hexane, stir with a glass rod at a constant speed for 3 min until the mixture is uniform, tighten the stopper to prevent solvent evaporation, let stand for 5 min, fix the Erlenmeyer flask in an ultrasonic cleaner, set the ultrasonic power to 300 W, ultrasonic time to 25 min, ultrasonic temperature to room temperature (25 ° C), start the equipment to perform ultrasonic extraction, and obtain the extract. After sonication, the flask was immediately placed in a boiling water bath for 5 minutes to rapidly inactivate the complex enzyme and terminate the enzymatic hydrolysis reaction. Then, it was taken out and cooled to room temperature. The cooled mixture was transferred to a centrifuge tube and placed in a centrifuge. It was centrifuged at 4500 r / min for 5 minutes to fully separate the solid and liquid. The supernatant containing essential oil was collected by pipette. The supernatant containing essential oil was transferred to a distillation flask in a rotary evaporator. The distillation temperature was set to 50℃ and the vacuum degree to -0.09MPa. The equipment was started for rotary evaporation to recover the n-hexane solvent until no solvent dripped from the distillation flask, thus obtaining crude linden wood Ganoderma lucidum spore essential oil. 5% anhydrous Na2SO4 (based on the mass of crude oil) was added to the crude essential oil, and the mixture was shaken and allowed to stand for 20 hours to fully absorb water. The sample was then transferred to a small centrifuge tube and centrifuged at 7000 r / min for 5 min to remove flocculent impurities and anhydrous Na2SO4 residue. The clear oily liquid at the top after centrifugation was collected, which is the linden wood Ganoderma lucidum spore essential oil with an extraction rate of 12.3%.

[0051] Example 4 A process for ultrasonic enzyme-assisted extraction of essential oil from Ganoderma lucidum spores is described. The other contents are the same as in Example 2, except that papain and cellulase are mixed in a mass ratio of 1:2 to obtain essential oil from Ganoderma lucidum spores with an extraction rate of 4.5%.

[0052] Example 5 A process for ultrasonic enzyme-assisted extraction of essential oil from Ganoderma lucidum spores is described. The other contents are the same as in Example 2, except that papain and cellulase are mixed in a mass ratio of 2:1 to obtain essential oil from Ganoderma lucidum spores with an extraction rate of 8.0%.

[0053] Comparative Example 1 An ultrasonic enzyme-assisted extraction process for Ganoderma lucidum spore essential oil is described. The other contents are the same as in Example 2, except that papain is not included. The resulting Ganoderma lucidum spore essential oil has an extraction rate of 4.1%.

[0054] Comparative Example 2 A process for ultrasonic enzyme-assisted extraction of Ganoderma lucidum spore essential oil is described. The other contents are the same as in Example 2, except that cellulase is not included. The resulting Ganoderma lucidum spore essential oil has an extraction rate of 6.2%.

[0055] The above examples and comparative examples illustrate that the composite enzyme (papain and cellulase in a specific ratio) of this application can precisely degrade the cell wall components of Ganoderma lucidum spores from linden wood, further breaking down the oil dissolution barrier. Ultrasound, through cavitation and mechanical vibration, accelerates the diffusion of active ingredients into the solvent, forming a synergistic mechanism of "enzymatic barrier breaking down - ultrasound-promoted dissolution." Simultaneously, key influencing factors such as the material-to-liquid ratio, ultrasonic time, and ultrasonic power were screened through single-factor experiments, and the parameter combination was optimized using the Box-Behnken response surface methodology to achieve precise control of the extraction process. This method can significantly improve the extraction rate and purity of Ganoderma lucidum spore essential oil from linden wood, effectively solving the problems of low efficiency, unreasonable parameter matching, and easy loss of active ingredients in traditional extraction techniques. It also reduces extraction costs and shortens the production cycle, providing technical support for the industrial-scale, efficient preparation of Ganoderma lucidum spore essential oil, with broad application prospects.

[0056] The above are merely preferred embodiments of the present invention, but the scope of protection of the present invention is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in the present invention should be included within the scope of protection of the present invention.

Claims

1. A process for ultrasonic enzyme-assisted extraction of essential oil from Ganoderma lucidum spores on linden wood, characterized in that, Includes the following steps: Linden wood Ganoderma lucidum spores, buffer solution and compound enzyme were mixed and subjected to water bath treatment to obtain enzymatic hydrolysis samples; An organic solvent was added to the enzymatically hydrolyzed sample, and the sample was subjected to ultrasonic treatment to obtain an extract. The extract was subjected to a boiling water bath and then centrifuged to obtain a supernatant containing essential oil. The supernatant containing the essential oil is subjected to rotary evaporation distillation, followed by drying to remove impurities, to obtain the linden wood Ganoderma lucidum spore essential oil.

2. The process for ultrasonic enzyme-assisted extraction of Ganoderma lucidum spore essential oil from linden wood according to claim 1, characterized in that, The buffer solution is a disodium hydrogen phosphate-citric acid buffer solution.

3. The process for ultrasonic enzyme-assisted extraction of Ganoderma lucidum spore essential oil from linden wood according to claim 1, characterized in that, The pH value of the buffer solution is 5-6.

4. The process for ultrasonic enzyme-assisted extraction of Ganoderma lucidum spore essential oil from linden wood according to claim 1, characterized in that, The complex enzyme is a mixture of papain and cellulase.

5. The process for ultrasonic enzyme-assisted extraction of Ganoderma lucidum spore essential oil from linden wood according to claim 1, characterized in that, The mass ratio of papain to cellulase is 1:1-1.

2.

6. The process for ultrasonic enzyme-assisted extraction of Ganoderma lucidum spore essential oil from linden wood according to claim 1, characterized in that, The total amount of the compound enzyme added is 1-2% of the mass of the Ganoderma lucidum spores.

7. The process for ultrasonic enzyme-assisted extraction of Ganoderma lucidum spore essential oil from linden wood according to claim 1, characterized in that, The water bath treatment is carried out at a temperature of 50-60℃ for 1 hour.

8. The process for ultrasonic enzyme-assisted extraction of Ganoderma lucidum spore essential oil from linden wood according to claim 1, characterized in that, The organic solvent is n-hexane.

9. The process for ultrasonic enzyme-assisted extraction of Ganoderma lucidum spore essential oil from linden wood according to claim 1, characterized in that, The ultrasonic treatment has a material-to-liquid ratio of 1:10-30, an ultrasonic time of 20-30 min, and an ultrasonic power of 200-400 W.

10. The process for ultrasonic enzyme-assisted extraction of Ganoderma lucidum spore essential oil from linden wood according to claim 1, characterized in that, The drying and impurity removal method is as follows: first, absorb water with anhydrous Na2SO4, and then remove impurities by centrifugation.