Molecular markers associated with lateral root number in alfalfa and their use

By designing KASP genotyping primers at SNP genotyping sites on alfalfa chromosome Chr5, the problems of long breeding cycles and environmental interference in traditional breeding methods were solved, achieving efficient screening and shortening the breeding cycle, and promoting the genetic improvement of the number of lateral roots in alfalfa.

CN122060912BActive Publication Date: 2026-07-24INSTITUTE OF ANIMAL SCIENCES OF CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INSTITUTE OF ANIMAL SCIENCES OF CHINESE ACADEMY OF AGRICULTURAL SCIENCES
Filing Date
2026-04-14
Publication Date
2026-07-24

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Abstract

The present application belongs to the technical field of molecular biology, and particularly relates to a molecular marker related to the number of lateral roots of Medicago sativa and application thereof. The purpose of the present application is to shorten the period of breeding alfalfa materials with excellent root system by finding a suitable molecular marker. The technical scheme of the present application is a molecular marker related to the number of lateral roots of Medicago sativa, and the specific information is as follows: chromosome: Chr5; position: 28984960; SNP typing: C / G. A KASP typing primer is designed for the site. A large number of materials are detected according to the designed KASP typing primer, and the results show that the number of lateral roots of different genotypes of materials has significant difference. The molecular marker and the KASP typing primer of the present application can complete the genotype screening at the seedling stage, and the selection can be performed without waiting for the plants to mature.
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