A molecular marker associated with a major QTL for cowpea resistance to Fusarium wilt, its primers and applications

By identifying the major QTL LFD-6.2 on chromosome 6 of cowpea for resistance to Fusarium wilt through genome-wide association analysis and developing the KASP marker, the problem of low breeding efficiency for cowpea resistance to Fusarium wilt was solved, enabling early and accurate breeding identification and shortening the breeding cycle.

CN122060918BActive Publication Date: 2026-06-30XIANGHU LABORATORY

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
XIANGHU LABORATORY
Filing Date
2026-04-20
Publication Date
2026-06-30

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Abstract

This invention discloses a molecular marker associated with a major QTL for resistance to Fusarium wilt in cowpea, its primers, and their applications, belonging to the field of plant molecular breeding technology. The associated KASP-type molecular marker 3_00160 is located at 46659973 bp on chromosome 6. This marker can be used to amplify test materials, and the genotype can be used to determine whether the sample carries the allelic variant controlling Fusarium wilt resistance. The KASP marker primer combination includes the following three sequences: Primer1 (sequence 2), Primer2 (sequence 3), and Primer_Common (sequence 4). Using the molecular marker and primers provided by this invention, rapid screening of major QTLs for resistance to Fusarium wilt in cowpea can be achieved, which is of great significance for breeding new disease-resistant varieties.
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