A lamp-crispr reaction tube, kit and method for detecting varicella-zoster virus

By combining LAMP-CRISPR detection with a carefully designed LAMP primer set and crRNA, a rapid, sensitive, and specific detection of varicella-zoster virus was achieved, solving the problems of long detection cycles and high equipment requirements in existing technologies. This method is suitable for primary healthcare institutions and home self-testing.

CN122060934BActive Publication Date: 2026-07-24SHENZHEN YILIFANG BIOTECH CO LTD +1
3 Cites 0 Cited by

Patent Information

Application Number
CN202610533881.6
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2026-04-22
Publication Date
2026-07-24
Estimated Expiration
2046-04-22

AI Technical Summary

Technical Problem

Existing technologies for varicella-zoster virus (VZV) detection suffer from problems such as long detection cycles, low sensitivity, high equipment requirements, complex operation, high detection costs, poor reaction system stability, and high non-specific amplification rates, making it difficult to meet the rapid and sensitive detection needs of primary healthcare institutions and home self-testing.

Method used

The LAMP-CRISPR combined detection method was adopted. By designing a carefully crafted LAMP primer set and crRNA, and combining the LAMP reaction system lyophilized microspheres and the CRISPR detection system lyophilized powder, a single-tube one-step detection method was achieved. The LAMP primer set was used to specifically amplify the VZV ORF62 gene, and the detection was performed with high sensitivity using the CRISPR/Cas12a system. A nucleic acid release agent was added to efficiently release viral nucleic acid.

Benefits of technology

It achieves rapid, sensitive, and highly specific VZV detection, with a detection time as fast as 35 minutes. It requires no complicated equipment and is suitable for primary healthcare institutions and home self-testing. The detection limit is as low as 100 copies/μL, which can effectively detect low viral load samples during the latent infection period.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The application discloses a kind of detection varicella-zoster virus LAMP-CRISPR reaction tube, kit and method, belong to biological detection technical field.The application provides a kind of detection varicella-zoster virus LAMP-CRISPR reaction tube, the reaction tube includes the LAMP reaction system freeze-dried microspheres placed in the bottom of pipe, and the CRISPR detection system freeze-dried powder sprayed on the pipe cover;The LAMP reaction system includes the LAMP primer group of nucleic acid sequence such as SEQ ID NO:2~7;The CRISPR detection system includes the crRNA of nucleic acid sequence such as SEQ ID NO:8.The LAMP-CRISPR reaction tube of the application uses "one-step method" single-tube closed-tube operation, only needs to be added to LAMP-CRISPR reaction tube after sample processing can carry out detection, and detection result is specific, and sensitivity is high.
Need to check novelty before this filing date? Find Prior Art