Sugarcane yellow rice borer sex attractant as well as preparation method and application thereof

Abstract

The invention discloses a sex attractant for yellow rice borers as well as a preparation method and application of the sex attractant, and relates to the technical field of agricultural pest monitoring, prevention and control. The sex attractant comprises an active component which is prepared from (Z)-dodecyl-9-ene-1-yl acetate, (E)-dodecyl-9, 11-diene-1-yl acetate and (7E, 9Z)-dodecyl-7, 9-diene-1-yl acetate, and the sex attractant is prepared from an active component which is prepared from the following components of (Z)-dodecyl-9-ene-1-yl acetate, (E)-dodecyl-9, 11-diene-1-yl acetate and (7E, 9Z)- According to the sex attractant disclosed by the invention, aiming at the current sugarcane area dominant population, the three active components have a synergistic effect, so that the trapping activity of the current sugarcane area dominant population is remarkably improved, and the problems that the trapping activity is low and the specificity is poor due to the fact that a control object is not matched with the dominant population in the existing sex attractant product are solved. Field trials show that the trapping quantity of the sex attractant provided by the invention on the yellow rice borers is obviously higher than that of a control group, the sex attractant has extremely high population specificity, and accurate monitoring and efficient trapping and killing of yellow rice borer populations can be realized.

Description

Technical Field

[0001] This invention relates to the field of agricultural pest monitoring and control technology, and more specifically to a sugarcane yellow twig pheromone, its preparation method, and its application. Background Technology

[0002] Sugarcane moth Chilo infuscatellus Snellen is one of the major borer pests of sugarcane. Due to climate change and adjustments in planting structure in recent years, it has become the dominant species in major producing areas, seriously threatening the yield and sugar quality of sugarcane.

[0003] For a long time, the control of sugarcane borers has relied on chemical pesticides, but the resulting problems of pesticide resistance, pesticide residues, and environmental pollution have become increasingly prominent, necessitating the development of green control technologies. Sex pheromones, as an environmentally friendly biological control method, have been applied in the monitoring and control of sugarcane borers. However, existing technologies mainly target the sugarcane stripe borer and the sugarcane two-spotted borer, resulting in a mismatch between the target species and the current dominant species, and exhibiting poor trapping activity and specificity against the sugarcane yellow borer. Furthermore, research on the effective components, precise formulation, and stability of sugarcane yellow borer sex pheromones is insufficient, and there are currently no effective products on the market with stable control effects.

[0004] Therefore, developing a sex pheromone specifically for the sugarcane yellow twig borer that has high trapping activity, long-lasting effect, and good stability, along with its corresponding application methods, has become a technical problem that urgently needs to be solved by those skilled in the art. Summary of the Invention

[0005] This invention provides a sex pheromone for the sugarcane yellow leech, its preparation method, and its application.

[0006] To solve the above-mentioned technical problems, this application adopts the following technical solution:

[0007] A sex pheromone for the sugarcane yellow leech, the sex pheromone comprising an active ingredient composed of (Z)-dodecano-9-en-1-yl acetate, (E)-dodecano-9,11-dien-1-yl acetate and (7E,9Z)-dodecano-7,9-dien-1-yl acetate, wherein the total concentration of the active ingredient is 50 μg / μL to 1 mg / μL, and the mass ratio of (Z)-dodecano-9-en-1-yl acetate, (E)-dodecano-9,11-dien-1-yl acetate and (7E,9Z)-dodecano-7,9-dien-1-yl acetate is 100:(3~5):(1~5).

[0008] Preferably, the total concentration of the active ingredient is 100ug / uL to 500ug / uL, and the mass ratio of (Z)-dodec-9-en-1-yl acetate, (E)-dodec-9,11-dien-1-yl acetate and (7E,9Z)-dodec-7,9-dien-1-yl acetate is 100:(3~4):(3~4).

[0009] More preferably, the total concentration of the active ingredient is 100 ug / uL, and the mass ratio of (Z)-dodecano-9-en-1-yl acetate, (E)-dodecano-9,11-dien-1-yl acetate and (7E,9Z)-dodecano-7,9-dien-1-yl acetate in the active ingredient is 100:3:(3~4).

[0010] Preferably, the sex attractant further includes a solvent and a sustained-release carrier.

[0011] More preferably, the solvent is n-hexane, and the sustained-release carrier is a polyurethane capillary.

[0012] Another object of the present invention is to provide a method for preparing a sex pheromone for the sugarcane yellow worm, wherein (Z)-dodecano-9-en-1-yl acetate, (E)-dodecano-9,11-dien-1-yl acetate and (7E,9Z)-dodecano-7,9-dien-1-yl acetate are mixed in the above mass ratio and dissolved in a solvent to adjust to the total concentration.

[0013] Preferably, the preparation method further includes the step of loading the sex attractant into a sustained-release carrier.

[0014] More preferably, the step of loading the sex attractant into the sustained-release carrier specifically involves folding a polyurethane capillary in half, slowly injecting the prepared sex attractant into one end of the capillary using a micropipette, then quickly passing the injected end over the outer flame of an alcohol lamp several times until the end softens from the heat, immediately clamping it with tweezers and holding it for several seconds to seal the end using a heat-sealing method; repeating the above heat-sealing operation on the other end to ensure the entire attractant core is sealed.

[0015] Another object of the present invention is to provide the application of the above-mentioned sugarcane yellow stem borer pheromone or the sugarcane yellow stem borer pheromone prepared by the above-mentioned preparation method in the control of sugarcane yellow stem borer.

[0016] Compared with the prior art, the present invention has the following beneficial effects: 1. Highly targeted and significantly enhanced trapping activity: This invention's sex pheromone attractant targets the dominant sugarcane borer population in current sugarcane growing areas. The three active ingredients work synergistically to significantly enhance the trapping activity against the sugarcane borer, solving the problem of low trapping activity and poor specificity in existing sex pheromone attractants due to the mismatch between the target species and the dominant population. Field trials show that the sex pheromone attractant provided by this invention traps significantly more sugarcane borers than the control, and exhibits extremely high population specificity, enabling precise monitoring and efficient trapping of the borer population.

[0017] 2. Optimized Mechanism of Action for More Thorough Pest Control: The sex pheromone provided by this invention not only effectively attracts and kills male insects, but more importantly, its precisely simulated natural sex pheromone components effectively interfere with normal mating communication between males and females, causing males to experience a "disorientation" effect, thereby significantly reducing the mating success rate of females and suppressing the occurrence and damage of the next generation of populations from the source. This dual mechanism of action, combining "attracting and killing" and "disorientation," overcomes the shortcomings of traditional single-target male-attracting strategies, which have limited effectiveness in controlling pests with multiple mating habits, achieving more effective suppression of the sugarcane yellow twig borer population.

[0018] 3. Green and environmentally friendly, with significant economic and social benefits: The technical solution of this invention can be implemented without the use of chemical pesticides throughout the entire process, ensuring the safety of the sugarcane field's ecological environment, natural enemies, and humans and livestock. There is no risk of pesticide residues, which meets the requirements of sustainable agricultural development. At the same time, it provides strong technical support for the high-quality development of the sugarcane industry, and has significant economic, social, and ecological benefits. Attached Figure Description

[0019] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0020] Figure 1 This is a schematic diagram of the sex attractant loaded onto the carrier according to an embodiment of the present invention; Figure 2 This is a schematic diagram of the placement of the sex pheromone carrier in the field after it has been placed in the trap, according to an embodiment of the present invention. Figure 3 This is a statistical chart showing the trapping effect of Embodiment 1 of the present invention; Figure 4 This is a statistical chart showing the trapping effect in Embodiment 2 of the present invention; Figure 5 This is a statistical chart showing the trapping effect in Embodiment 3 of the present invention; Figure 6 This is a statistical chart showing the trapping effect in Embodiment 4 of the present invention; in, Figures 3-5 In this diagram, the letters above the bars represent the statistical significance of differences between groups. Groups labeled with the same letter indicate no significant difference between groups (e.g., both labeled "a", indicating p>0.05); groups labeled with different letters indicate significant differences between groups (e.g., one labeled "a" and the other "b", indicating p<0.05); combinations of multiple letters indicate partially overlapping differences between groups (e.g., a bar labeled "ab" indicates no significant difference between it and the group labeled "a" only, nor a significant difference between it and the group labeled "b" only, but a significant difference exists between the two groups labeled "a" and "b", a situation commonly seen in multiple comparisons). Detailed Implementation

[0021] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0022] Unless otherwise specified, the experimental methods used in the following examples are conventional methods, performed according to the techniques or conditions described in the literature in this field or according to the product instructions. Unless otherwise specified, the materials and reagents used in the following examples are commercially available and their sources are not specifically limited. For example, the (Z)-dodec-9-en-1-yl acetate [(Z)-Dodec-9-en-1-yl acetate, specification 1g, purity 90%], (E)-dodec-9,11-dien-1-yl acetate [(E)-Dodeca-9,11-dien-1-yl acetate, specification 100mg, purity 98%], and (7E,9Z)-dodec-7,9-dien-1-yl acetate [(7E,9Z)-Dodeca-7,9-dien-1-yl acetate, specification 250mg, purity ≥98%] of the present invention can all be purchased from the Macklin official website (http: / / www.macklin.cn / ).

[0023] Experimental location for this invention: Liangqi Farm, Nanning City; The implementation period of this invention is from April 1, 2025 to December 31, 2025.

[0024] Example 1: Preliminary screening of sugarcane yellow leech sex pheromone components According to the ratios in Table 1, (Z)-dodec-9-en-1-yl acetate, (E)-dodec-9,11-dien-1-yl acetate, and (7E,9Z)-dodec-7,9-dien-1-yl acetate were prepared into single or mixed solutions. The total concentration of the active sex pheromone components in groups T1 to T11 was 1 mg / uL, the total concentration of the active sex pheromone components in groups T1-1 to T3-1 was 0.05 mg / uL, and group C was a blank control group without sex pheromone components. The solvent in all the above groups was n-hexane.

[0025] Sex attractant loading: The sustained-release carrier for the sex attractant is a polyurethane capillary tube (material). The preparation steps are as follows: First, according to the experimental design requirements, weigh the specific ratio of the active sex pheromone component, mix it with n-hexane solvent, and shake thoroughly to completely dissolve the active component, obtaining a homogeneous sex pheromone solution. Then, select a polyurethane capillary tube with a length of 8-10 cm (outer diameter 2 mm, inner diameter 1 mm), and fold it in half to form a "U" shape. Accurately measure 20 μL of the prepared solution using a micropipette and slowly inject it into one end of the capillary tube. Next, quickly sweep the injected end of the tube several times over the outer flame of an alcohol lamp until the end softens from the heat, then immediately clamp it with tweezers and hold it for several seconds to seal the end using a heat-sealing method. Repeat the heat-sealing operation on the other end to ensure the entire attractant core is sealed, thus obtaining the finished attractant core (see Appendix). Figure 1 The lure has a simple structure and can be adapted to various types of field traps.

[0026] Field trapping experiment: The prepared lure was placed in the trap (see appendix). Figure 2 The traps were placed in the sugarcane field at a height of 50-80 cm above the ground. Each treatment was repeated three times (evenly distributed within one acre), with treatments randomly arranged and at least 30 meters apart. Surveys were conducted daily, and after recording data, the number of male sugarcane borers trapped in the traps was removed. Results were tallied after 30 days (see appendix). Figure 3 ).

[0027] Number of insects trapped = (repeated 1 + repeated 2 + repeated 3) / 3 ± standard deviation, where repeated 1, repeated 2, and repeated 3 are the total number of insects trapped by a single trap over 30 days.

[0028] Table 1. Sex attractant ratios

[0029] From the appendix Figure 3It is known that (Z)-dodec-9-en-1-yl acetate and (E)-dodec-9,11-dien-1-yl acetate, when used alone, have a certain trapping effect on the sugarcane borer. When the two are used in combination in a specific ratio [total concentration of 1 mg / μL, mass ratio of 100:(1~20), preferably 100:(3~5)], the effect is significantly enhanced. However, (7E,9Z)-dodec-7,9-dien-1-yl acetate, when used alone, does not show a trapping effect on the sugarcane borer.

[0030] Example 2: Optimization of Sugarcane Yellow Leaf Borer Components Based on the preferred experimental results in Example 1, the compounding scheme of (7E,9Z)-dodecano-7,9-diene-1-ylacetate was further studied. The mass ratio of the three sex pheromone active ingredients is shown in Table 2, and the total concentration is 1 mg / μL. The methods for loading the sex attractant into the slow-release carrier and the sugarcane field trapping experiment were the same as in Example 1, and the results are shown in Appendix. Figure 4 .

[0031] Table 2. Sex attractant ratios

[0032] From the appendix Figure 4 It is known that when (7E,9Z)-dodecano-7,9-dien-1-yl acetate is added to the compound system of (Z)-dodecano-9-dien-1-yl acetate and (E)-dodecano-9,11-dien-1-yl acetate in a specific ratio, it further promotes the trapping effect of the sex pheromone on the sugarcane leafminer, and the effect is significant. The total concentration of the active sex pheromone component in the sex pheromone is 1 mg / uL, and the mass ratio of (Z)-dodecano-9-dien-1-yl acetate, (E)-dodecano-9,11-dien-1-yl acetate to (7E,9Z)-dodecano-7,9-dien-1-yl acetate is 100:(3~5):(1~5), preferably 100:(3~4):(3~4), and more preferably 100:3:(3~4).

[0033] Example 3: Optimization of Sugarcane Yellow Leaf Borer Concentration This embodiment, based on the experimental results of Example 2, optimized the T29 and T30 formulations for attracting male adult sugarcane borers. The attraction effects of different dosages of sex pheromones were compared. The mass ratios of T29 and T30 were used to prepare sex pheromones of different total concentrations according to Table 3 for experiments. The methods for loading the sex pheromones onto the slow-release carrier and the sugarcane field trapping experiments were the same as in Example 1. The results are shown in the appendix. Figure 5 .

[0034] Table 3

[0035] From the appendix Figure 5 It is known that the attraction effect of different total concentrations of sex pheromone varies. As the initial total concentration decreases (T47-T50 and T53-T55), the number of male adult sugarcane borers attracted shows an increasing trend. However, when the concentration of sex pheromone is below 100 ug / uL, the number of male adult sugarcane borers attracted shows a significant decreasing trend as the concentration of sex pheromone decreases. However, at 50 ug / uL, it can still achieve an effect comparable to 1 mg / uL. Therefore, the suitable range of the total concentration of the sex pheromone for sugarcane borers in this invention is 50 ug / uL to 1 mg / uL, preferably 100 ug / uL to 500 ug / uL, and more preferably 100 ug / uL.

[0036] Example 4 In this embodiment, formulation T50 [i.e., total concentration of sex attractant 100 μg / μL, wherein the mass ratio of (Z)-dodecano-9-en-1-yl acetate, (E)-dodecano-9,11-dien-1-yl acetate and (7E,9Z)-dodecano-7,9-dien-1-yl acetate is 100:3:3] was used in a continuous three-month experiment (preparation method refers to Example 1) to test the stability of the formulation. The experimental results are attached. Figure 6 As shown, there were no significant differences in the number of male sugarcane borers attracted in August, September and October, and all remained at a high level.

[0037] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on the differences from other embodiments. The same or similar parts between the various embodiments can be referred to each other.

[0038] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A sex pheromone for the sugarcane yellow twig borer, characterized in that, The sex attractant comprises an active ingredient consisting of (Z)-dodecano-9-en-1-yl acetate, (E)-dodecano-9,11-dien-1-yl acetate, and (7E,9Z)-dodecano-7,9-dien-1-yl acetate, with a total concentration of 50 μg / μL to 1 mg / μL, and the mass ratio of (Z)-dodecano-9-en-1-yl acetate, (E)-dodecano-9,11-dien-1-yl acetate, and (7E,9Z)-dodecano-7,9-dien-1-yl acetate is 100:(3~5):(1~5).

2. The sugarcane yellow leech pheromone attractant according to claim 1, characterized in that, The total concentration of the active ingredient is 100ug / uL to 500ug / uL, and the mass ratio of (Z)-dodecano-9-en-1-yl acetate, (E)-dodecano-9,11-dien-1-yl acetate and (7E,9Z)-dodecano-7,9-dien-1-yl acetate is 100:(3~4):(3~4).

3. The sugarcane yellow leech pheromone attractant according to claim 2, characterized in that, The total concentration of the active ingredient is 100 ug / uL, and the mass ratio of (Z)-dodec-9-en-1-yl acetate, (E)-dodec-9,11-dien-1-yl acetate and (7E,9Z)-dodec-7,9-dien-1-yl acetate is 100:3:(3~4).

4. The sugarcane yellow leech pheromone attractant according to any one of claims 1-3, characterized in that, The sex attractant also includes a solvent and a sustained-release carrier.

5. The sugarcane yellow leech pheromone attractant according to claim 4, characterized in that, The solvent is n-hexane, and the sustained-release carrier is a polyurethane capillary.

6. A method for preparing a sex pheromone for the sugarcane yellow twig borer, characterized in that, (Z)-dodec-9-en-1-yl acetate, (E)-dodec-9,11-dien-1-yl acetate and (7E,9Z)-dodec-7,9-dien-1-yl acetate are mixed in the mass ratios described in any one of claims 1-3 and dissolved in a solvent to adjust to the total concentration.

7. The method for preparing the sugarcane yellow leech pheromone according to claim 6, characterized in that, It also includes the step of loading the sex pheromone into a sustained-release carrier.

8. The method for preparing the sugarcane yellow leech pheromone according to claim 7, characterized in that, The specific steps of loading the sex attractant into the sustained-release carrier are as follows: fold the polyurethane capillary in half, use a micropipette to slowly inject the prepared sex attractant from one end of the capillary, then quickly sweep the injected end over the outer flame of an alcohol lamp several times. After the end softens from the heat, immediately clamp it with tweezers and hold it for a few seconds to seal the end using a heat-sealing method; repeat the above heat-sealing operation on the other end to ensure that the entire attractant core is sealed.

9. The application of the sugarcane yellow stem borer pheromone according to any one of claims 1-5 or the sugarcane yellow stem borer pheromone prepared by any one of the preparation methods of claims 6-8 in the control of sugarcane yellow stem borer.

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