Preparation method of sheep-strengthening powder

Abstract

The invention belongs to the technical field of feed additives, and particularly relates to a preparation method of sheep Zhuang powder, which comprises the following specific steps: preparing an astragalus extract, preparing berberine hydrochloride, and mixing for preparation. In the preparation process of the sheep strengthening powder, the feed additive is added, the feed additive effectively replaces the previous aureomycin, the antibacterial effect (MIC) of the feed additive on escherichia coli AZ1, escherichia coli 8-1 and salmonella sk77 is obviously better than that of the aureomycin, the dosage every time is smaller, animals are healthier, both pathogenic bacteria are killed, the immunity is improved, and the sheep strengthening powder is suitable for being used for preparing sheep strengthening powder. The feed is safe and compliant, has no residue and no withdrawal period, and has the effects of fattening and weight gaining and fierce eating and fierce growth.

Description

Technical Field

[0001] The present invention relates to the technical field of feed additives, and specifically to a preparation method of Yangzhuang Powder. Background Art

[0002] The existing Yangzhuang Powder still has the following technical problems when in use: Currently, Chinese herbal medicine product additives are effectively used to replace antibiotics. Chinese herbal medicine products are widely used in aspects such as anti-inflammatory, antibacterial, and disease resistance. There have been research and comparative tests on costs using Chinese herbal medicine products and chlortetracycline, etc. The weight gain effects of meat sheep are the same. The detection results of the serum biochemical indexes of meat sheep using Chinese herbal medicine products to replace chlortetracycline show that the former can reduce the content of alanine aminotransferase ALT in the serum, having a liver protection effect. At the same time, it can increase the content of glucose GLU in the serum, ensuring the energy supply of the body and not affecting the stability of electrolytes in the body of meat sheep; the influence results of replacing chlortetracycline with Chinese herbal medicine products on the immune function parameters of meat sheep show that the former can effectively promote the production of total protein TP and albumin ALB in the serum of meat sheep, improving the metabolic level and immunity of the body. It can promote the production of the contents of IgG and IgM in the serum of meat sheep, improving the immunity of meat sheep and promoting the production of immune factors; replacing antibiotics to play anti-inflammatory, promoting repair and regulating the body function.

[0003] Therefore, a preparation method of Yangzhuang Powder is proposed to solve the above-mentioned problems. Summary of the Invention

[0004] The purpose of the present invention is to provide a preparation method of Yangzhuang Powder to solve the problems raised in the above background art.

[0005] To achieve the above purpose, the present invention provides the following technical solution: A preparation method of Yangzhuang Powder, and the specific steps of this preparation method are as follows: Step 1: Prepare astragalus extract: Weigh the required astragalus and put it into an extraction tank, inject 3 times the amount of 1% Cao aqueous solution and soak for 2 hours, then reflux and extract with 100 °C steam for 1 hour, collect and filter the extraction solution, repeat the extraction 3 times in the same method, collect the total extraction solution and perform vacuum double-effect concentration. After concentrating to a ratio of crude drug to concentrated solution of 1:1, add 95% ethanol and stir, then stand for 24 hours. Draw the supernatant and recover it with an alcohol recovery tower until the alcohol concentration is less than 60% and then stop reflux recovery. Collect the precipitate, dissolve it in water, and pump it into a spray dryer for spray drying to obtain astragalus powder extract; Step 2: Preparation of berberine hydrochloride: Weigh the required amount of Coptis chinensis, crush it into coarse powder, add 3 times the amount of 95% ethanol and soak for 48 hours. Filter the soaking solution, add an appropriate amount of hydrochloric acid to adjust the pH value to about 3.0, let it stand for 12 hours to precipitate, wash the precipitate with water 3 times, add 2 times the amount of water and adjust the pH value of the filtrate to 8.0-9.0 with 15% NaOH, let it stand for precipitate for another 12 hours, absorb the supernatant and add 5-6% salt to the supernatant to precipitate berberine in the concentrated salt solution. Finally, centrifuge and collect to obtain berberine hydrochloride. Step 3, Mixing and Preparation: Weigh the required raw materials according to the following weight proportions: 20-30 parts of Astragalus extract, 3-8 parts of berberine hydrochloride, 17-25 parts of compound small molecule peptides, 8-12 parts of feed-grade calcium magnesium carbonate powder, and 18-20 parts of feed additives. Mix them in two dimensions to obtain Yangzhuang powder.

[0006] Preferably, the specific steps for preparing this feed additive are as follows: Step 1: Select raw materials: Select 5-8 parts of Centella asiatica extract, 8-12 parts of Codonopsis pilosula extract, 20-30 parts of Portulaca oleracea powder, 3-5 parts of compound small molecule peptides, 15-20 parts of activated beneficial bacteria liquid, 2-4 parts of intestinal villus growth promoter, and an appropriate amount of feed-grade calcium magnesium carbonate powder. Step 2: Mixing solid components: Take purslane powder, centella asiatica extract, codonopsis extract, compound small molecule peptides, intestinal villus growth promoter and feed-grade calcium magnesium carbonate powder, put them into a double helix mixer and mix at room temperature for 15 minutes to obtain basic mixed powder. Step 3, Gradual addition of liquid components: Slowly add the activated beneficial bacteria solution to the basic mixed powder, stir for 20 minutes to ensure that the bacteria solution is evenly dispersed; Step 4: Low-temperature fermentation and drying: Transfer the mixture to a fermentation tank and ferment for 24 hours at 30-32℃ and 60-70% humidity; after fermentation, use vacuum freeze drying to dry until the moisture content is ≤6%. Step 5: Crushing and screening: The dried material is crushed and passed through a 100-mesh sieve to remove agglomerated particles, resulting in a uniform powdered additive.

[0007] Preferably, before selecting the raw materials in step one, the raw materials need to be pre-treated. The specific steps are as follows: select fresh purslane, rinse with clean water to remove impurities, dry at a low temperature of 60°C until the moisture content is ≤8%, pulverize and pass through an 80-mesh sieve to obtain purslane dry powder. Centella asiatica extract and Codonopsis pilosula extract were purified by high-performance liquid chromatography (HPLC) to ensure the absence of heavy metal residues; beneficial bacteria were inoculated into sterile culture medium and incubated at 37°C for 12 hours, with the activated bacterial concentration ≥ CFU / mL, for later use.

[0008] Preferably, during the solid component mixing process in step two, the rotation speed of the twin-helix mixer is controlled at 200-300 r / min.

[0009] Preferably, in step three, after the bacterial solution is dispersed, the material humidity is controlled to be ≤15% to prevent the live bacteria from becoming moldy.

[0010] Preferably, in step four, the temperature for vacuum freeze drying after fermentation is -40℃, and the vacuum degree is 0.06-0.08MPa.

[0011] Preferably, after the powdered additive is prepared, it needs to be subjected to quality testing. The testing indicators are as follows: total viable bacteria count ≥ CFU / g, asiaticoside ≥0.8%, Codonopsis pilosula polysaccharide ≥1.5%, moisture ≤6%, total mold count ≤ CFU / g.

[0012] Compared with the prior art, the beneficial effects of the present invention are: The sheep strengthening powder in this application incorporates feed additives during its preparation process. These feed additives effectively replace the previously used chlortetracycline. The inhibitory effect (MIC) of the feed additives on Escherichia coli AZ1, Escherichia coli 8-1, and Salmonella SK77 is significantly stronger than that of chlortetracycline. The dosage is lower each time, resulting in healthier animals. It kills both pathogens, improves immunity, is safe and compliant, leaves no residue, has no withdrawal period, and promotes fattening and weight gain, resulting in rapid growth. Attached Figure Description

[0013] Figure 1 This is an antibacterial diagram of the feed additive in this invention; Figure 2 A comparative chart showing the effects of adding feed additives to the diet on the growth performance and diarrhea rate of meat sheep. Figure 3 Flowchart of the preparation steps for Yangzhuangsan.

[0014] In the figure: Jinmeitai is the name of the feed additive in this application. Detailed Implementation

[0015] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0016] Example:

[0017] Please see Figure 1-2 The present invention provides a technical solution: A method for preparing Yangzhuang Powder, the specific steps of which are as follows: Step 1: Preparation of Astragalus Extract: Weigh the required amount of Astragalus and put it into the extraction tank. Add 3 times the amount of 1% CaO aqueous solution and soak for 2 hours. Then, extract by reflux with 100℃ steam for 1 hour. Collect the filtered extract and repeat the extraction 3 times. Collect the total extract and perform vacuum double-effect concentration. After concentration to a ratio of raw drug to concentrate of 1:1, add 95% ethanol and stir. Let stand for 24 hours. Extract the supernatant and recover it with an alcohol recovery tower until the alcohol concentration is less than 60%. Stop the reflux recovery. Collect the precipitate, dissolve it in water, and then spray it into a spray dryer for spray drying to obtain Astragalus powder extract. Step 2: Preparation of berberine hydrochloride: Weigh the required amount of Coptis chinensis, crush it into coarse powder, add 3 times the amount of 95% ethanol and soak for 48 hours. Filter the soaking solution, add an appropriate amount of hydrochloric acid to adjust the pH value to about 3.0, let it stand for 12 hours to precipitate, wash the precipitate with water 3 times, add 2 times the amount of water and adjust the pH value of the filtrate to 8.0-9.0 with 15% NaOH, let it stand for precipitate for another 12 hours, absorb the supernatant and add 5-6% salt to the supernatant to precipitate berberine in the concentrated salt solution. Finally, centrifuge and collect to obtain berberine hydrochloride. Step 3, Mixing and Preparation: Weigh the required raw materials according to the following weight proportions: 20-30 parts of Astragalus extract, 3-8 parts of berberine hydrochloride, 17-25 parts of compound small molecule peptides, 8-12 parts of feed-grade calcium magnesium carbonate powder, and 18-20 parts of feed additives. Mix them in two dimensions to obtain Yangzhuang powder.

[0018] The effects and benefits of Astragalus extract: It replenishes qi and strengthens the spleen, improves appetite and increases food intake: It can improve problems such as poor appetite in sheep, failure to eat after giving birth or after illness, and slow growth even when eating dry food. It can increase feed intake, promote digestion and absorption, and reduce diarrhea caused by uneaten feed.

[0019] Enhances the body's immunity: Promotes the production of total protein (TP), albumin (ALB), IgG, and IgM in sheep, improves disease resistance, reduces the incidence of respiratory and intestinal diseases, and replaces antibiotics to achieve "antibiotic-free disease prevention".

[0020] Protects the liver and reduces metabolic burden: Lowers serum alanine aminotransferase (ALT), protects the liver, enhances liver detoxification and metabolism, and makes sheep healthier with long-term feeding.

[0021] Anti-stress and stabilize physical condition: alleviates stress caused by long-distance transportation, relocation, environmental changes, and vaccination, and reduces problems such as decreased food intake, weight stagnation, and physical weakness.

[0022] Promotes growth and weight gain: Improves nutrient metabolism and blood circulation, accelerates bone development and muscle deposition, increases daily weight gain, shortens the time to market, and makes sheepskin and wool smooth and shiny.

[0023] The effects and efficacy of berberine hydrochloride: Natural broad-spectrum antibacterial agent, replacing chlortetracycline: It has a strong inhibitory effect on pathogenic bacteria such as Escherichia coli and Salmonella, with a clear antibacterial effect, no drug resistance, and no drug residue.

[0024] Clears heat and dries dampness, stops diarrhea and dysentery: Quickly improves diarrhea, dysentery and bacterial enteritis in sheep, reduces the diarrhea rate and protects intestinal health.

[0025] Anti-inflammatory and mucosal repair effects in the intestines: Reduces intestinal inflammatory response, works synergistically with purslane and centella asiatica to repair damaged intestinal mucosa and improve intestinal barrier function.

[0026] Regulates intestinal microecology: inhibits the growth of harmful bacteria, creates a favorable environment for the colonization of beneficial bacteria, reduces intestinal flora imbalance, and improves feed digestibility and utilization.

[0027] Safe, residue-free, and with no withdrawal period: It is a natural plant-derived antibacterial ingredient that does not contaminate meat or the environment, making it suitable for long-term addition throughout the entire process.

[0028] The specific steps for preparing this feed additive are as follows: Step 1: Select raw materials: Select 5-8 parts of Centella asiatica extract, 8-12 parts of Codonopsis pilosula extract, 20-30 parts of Portulaca oleracea powder, 3-5 parts of compound small molecule peptides, 15-20 parts of activated beneficial bacteria liquid, 2-4 parts of intestinal villus growth promoter, and an appropriate amount of feed-grade calcium magnesium carbonate powder. Step 2: Mixing solid components: Take purslane powder, centella asiatica extract, codonopsis extract, compound small molecule peptides, intestinal villus growth promoter and feed-grade calcium magnesium carbonate powder, put them into a double helix mixer and mix at room temperature for 15 minutes to obtain basic mixed powder. Step 3, Gradual addition of liquid components: Slowly add the activated beneficial bacteria solution to the basic mixed powder, stir for 20 minutes to ensure that the bacteria solution is evenly dispersed; Step 4: Low-temperature fermentation and drying: Transfer the mixture to a fermentation tank and ferment for 24 hours at 30-32℃ and 60-70% humidity; after fermentation, use vacuum freeze drying to dry until the moisture content is ≤6%. Step 5: Crushing and screening: The dried material is crushed and passed through a 100-mesh sieve to remove agglomerated particles, resulting in a uniform powdered additive.

[0029] The core functions of Centella asiatica extract are anti-inflammatory, mucosal repair, liver protection, and antibiotic alternative. Specifically, it reduces alanine aminotransferase (ALT) in the serum of sheep, thus protecting the liver; it has anti-inflammatory and anti-stress effects, repairs damage to the intestinal and body mucosa, and replaces the anti-inflammatory function of antibiotics; it enhances the body's resistance and, in combination with other ingredients, strengthens the overall disease resistance.

[0030] The core functions of Codonopsis pilosula extract are to enhance immunity, promote protein synthesis, and boost metabolism. Specifically, it promotes the production of serum total protein (TP) and albumin (ALB), thereby improving the body's metabolic level; it promotes the production of immunoglobulins such as IgG and IgM, significantly enhancing the immunity of meat sheep; it invigorates qi and strengthens the spleen, improving physical condition, reducing disease incidence, and achieving disease resistance under antibiotic-free farming. The core function of purslane powder is its antibacterial and anti-inflammatory properties, as well as its ability to protect the intestines and stop diarrhea, all characteristic of traditional Chinese medicine. Specifically, it has the effects of naturally clearing heat and detoxifying, antibacterial and anti-inflammatory, and significantly improving intestinal inflammation; reducing diarrhea and protecting intestinal health; and acting as a traditional Chinese medicine ingredient to synergistically replace antibiotics; it contains active substances such as polysaccharides and flavonoids, which help enhance the body's immunity.

[0031] The core functions of compound small molecule peptides are highly effective antibacterial activity, non-drug resistance, and immune regulation. Specifically, it has the effects of broad-spectrum killing of pathogenic bacteria and is the core functional component to replace antibiotics; it does not produce drug resistance or drug residues, meeting the requirements of green farming; it regulates intestinal flora, enhances immune response, and improves disease resistance.

[0032] The core function of activated beneficial bacteria liquid is to regulate the intestinal microecology, promote digestion, and enhance immunity; the specific effects are to colonize the intestine, inhibit the reproduction of harmful bacteria, maintain the balance of intestinal flora, improve feed digestibility and absorption, reduce intestinal diseases, stimulate intestinal immunity, and enhance the body's overall immunity.

[0033] The core function of intestinal villus growth promoters is to promote the growth of intestinal villus, improve absorption, and strengthen the barrier; promote the growth and thickening of intestinal villus, expanding the nutrient absorption area; strengthen the intestinal mucosal barrier and reduce pathogen invasion; directly improve feed conversion efficiency and accelerate growth.

[0034] The purpose of feed-grade calcium and magnesium carbonate powder is to increase trace elements. Adding trace elements can reduce the problem of flaccid paralysis in sheep and enhance myocardial vitality.

[0035] The results of this application, in which the herbal product was used as a substitute for chlortetracycline in the detection of serum biochemical indicators in sheep, showed that the former could reduce the content of alanine aminotransferase (ALT) in serum, thus having a hepatoprotective effect. Simultaneously, it could increase the content of glucose (GLU) in serum, ensuring the body's energy supply without affecting the stability of electrolytes in the sheep. The results of this application regarding the effects of the traditional Chinese medicine product as a substitute for chlortetracycline on the immune function parameters of mutton sheep show that the former can effectively promote the production of total protein (TP) and albumin (ALB) in mutton sheep serum, thereby improving the body's metabolic level and immunity. It can also promote the production of IgG and IgM in mutton sheep serum, thus enhancing the sheep's immunity and promoting the production of immune factors.

[0036] Before selecting the raw materials, the raw materials need to be pre-treated in the first step. The specific steps are as follows: Select fresh purslane, rinse with clean water to remove impurities, dry at a low temperature of 60℃ until the moisture content is ≤8%, pulverize and pass through an 80-mesh sieve to obtain purslane dry powder. Centella asiatica extract and Codonopsis pilosula extract were purified by high-performance liquid chromatography (HPLC) to ensure the absence of heavy metal residues; beneficial bacteria were inoculated into sterile culture medium and incubated at 37°C for 12 hours, with the activated bacterial concentration ≥ CFU / mL, for later use.

[0037] In step two, during the mixing of solid components, the rotation speed of the twin-helix mixer is controlled at 200-300 r / min. This moderate rotation speed ensures uniform mixing of the solid raw materials without stratification, while preventing damage to the material structure and dust generation due to excessive speed; thus guaranteeing the uniformity and stability of the base mixed powder. In step three, after the bacterial solution is dispersed, the material humidity is controlled to ≤15% to prevent mold growth of the live bacteria. Controlling humidity prevents mold growth and contamination by other microorganisms; it also ensures the survival rate of beneficial bacteria and avoids spoilage and inactivation during fermentation / storage. In step four, the temperature for vacuum freeze-drying after fermentation is -40℃, and the vacuum degree is 0.06-0.08MPa. Rapid drying in an ultra-low temperature and high vacuum environment maximizes the retention of heat-sensitive active ingredients such as asiaticoside, codonopsis polysaccharides, complex small molecule peptides, and live bacteria; the moisture content is precisely controlled to ≤6%, achieving long-term stable storage. After the powdered additive is prepared, it needs to be subjected to quality testing. The testing indicators are as follows: total viable bacteria count ≥ CFU / g, asiaticoside ≥0.8%, Codonopsis pilosula polysaccharide ≥1.5%, moisture ≤6%, total mold count ≤ CFU / g. Total viable bacteria count meets standards: ensuring the effects of intestinal regulation and improved immunity; Centella asiatica extract and Codonopsis pilosula polysaccharide meet standards: ensuring the core effects of liver protection, anti-inflammation, and increasing serum protein and immunoglobulin; Moisture and total mold count meet standards: ensuring the product is safe, non-toxic, and mold-free, and complies with feed additive regulations; overall, ensuring that the additive can stably replace antibiotics.

[0038] Application example: 366 healthy fattening sheep were selected and divided into a control group of 183 sheep and an experimental group of 183 sheep. The experiment lasted for 30 days. The sheep were fed according to the experimental diet. The control group was fed the basal diet, while the experimental group was fed the basal diet plus 2g / sheep / day of this product (1000g / 500 sheep / day). The feed intake, daily weight gain and diarrhea rate of the sheep were recorded.

[0039] The results are as follows Figure 2Compared with the control group, the average feed intake of the group using the herbal products in this application increased from 2.36 jin to 2.89 jin, an increase of 0.53 jin; the average daily weight gain increased from 0.58 jin to 0.71 jin, an increase of 0.13 jin; and the diarrhea rate decreased from 6.6% to 1.6%.

[0040] Conclusion: The product in this application contains Centella asiatica extract, Codonopsis pilosula extract, Astragalus membranaceus extract, berberine hydrochloride, and complex small molecule peptides. Centella asiatica extract regulates intestinal flora balance and enhances intestinal immunity. Compared with ordinary products, this product has a 20% higher digestibility and absorption rate, significantly reducing feed waste. Taking fattening sheep as an example, it can more efficiently convert feed into weight gain. Codonopsis pilosula extract has the effects of tonifying qi and strengthening the spleen, nourishing blood and promoting body fluid production, while also regulating immunity and protecting organs.

[0041] The daily weight gain of fattening sheep using the product described in this application can reach 0.7-0.8 kg, while that of ordinary breeding methods is only 0.5-0.6 kg, an increase of up to 40%. After using the product described in this application, the lambing survival rate of ewes reaches 92%, which is 15% higher than the 80% of ordinary breeding methods. The feed conversion ratio of the product described in this application is 3.5:1, while that of ordinary breeding methods is 4.5:1, which reduces the feed conversion ratio by 22%. The fattening sheep using the product described in this application have a market cycle of 5-6 months, while those raised in ordinary methods require 6-7 months, shortening the market cycle by 25%.

[0042] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or basic characteristics. Therefore, the embodiments should be considered exemplary and non-limiting in all respects. The scope of the invention is defined by the appended claims rather than the foregoing description. Therefore, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the present invention, and no reference numerals in the claims should be construed as limiting the scope of the claims.

[0043] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A method for preparing Yangzhuang Powder, characterized in that, The specific steps of this preparation method are as follows: Step 1: Preparation of Astragalus Extract: Weigh the required amount of Astragalus and put it into the extraction tank. Add 3 times the amount of 1% CaO aqueous solution and soak for 2 hours. Then, extract by reflux with 100℃ steam for 1 hour. Collect the filtered extract and repeat the extraction 3 times. Collect the total extract and perform vacuum double-effect concentration. After concentration to a ratio of raw drug to concentrate of 1:1, add 95% ethanol and stir. Let stand for 24 hours. Extract the supernatant and recover it with an alcohol recovery tower until the alcohol concentration is less than 60%. Stop the reflux recovery. Collect the precipitate, dissolve it in water, and then spray it into a spray dryer for spray drying to obtain Astragalus powder extract. Step 2: Preparation of berberine hydrochloride: Weigh the required amount of Coptis chinensis, crush it into coarse powder, add 3 times the amount of 95% ethanol and soak for 48 hours. Filter the soaking solution, add an appropriate amount of hydrochloric acid to adjust the pH value to about 3.0, let it stand for 12 hours to precipitate, wash the precipitate with water 3 times, add 2 times the amount of water and adjust the pH value of the filtrate to 8.0-9.0 with 15% NaOH, let it stand for precipitate for another 12 hours, absorb the supernatant and add 5-6% salt to the supernatant to precipitate berberine in the concentrated salt solution, and finally collect by centrifugation to obtain berberine hydrochloride. Step 3, Mixing and Preparation: Weigh the required raw materials according to the following weight proportions: 20-30 parts of Astragalus extract, 3-8 parts of berberine hydrochloride, 17-25 parts of compound small molecule peptides, 8-12 parts of feed-grade calcium magnesium carbonate powder, and 18-20 parts of feed additives. Mix them in two dimensions to obtain Yangzhuang powder.

2. The method for preparing a sheep-strengthening powder according to claim 1, characterized in that: The specific steps for preparing this feed additive are as follows: Step 1: Select raw materials: Select 5-8 parts of Centella asiatica extract, 8-12 parts of Codonopsis pilosula extract, 20-30 parts of Portulaca oleracea powder, 3-5 parts of compound small molecule peptides, 15-20 parts of activated beneficial bacteria liquid, 2-4 parts of intestinal villus growth promoter, and an appropriate amount of feed-grade calcium magnesium carbonate powder. Step 2: Mixing solid components: Take purslane powder, centella asiatica extract, codonopsis extract, compound small molecule peptides, intestinal villus growth promoter and feed-grade calcium magnesium carbonate powder, put them into a double helix mixer and mix at room temperature for 15 minutes to obtain basic mixed powder. Step 3, Gradual addition of liquid components: Slowly add the activated beneficial bacteria solution to the basic mixed powder, stir for 20 minutes to ensure that the bacteria solution is evenly dispersed; Step 4: Low-temperature fermentation and drying: Transfer the mixture to a fermentation tank and ferment for 24 hours at 30-32℃ and 60-70% humidity; after fermentation, use vacuum freeze drying to dry until the moisture content is ≤6%. Step 5: Crushing and screening: The dried material is crushed and passed through a 100-mesh sieve to remove agglomerated particles, resulting in a uniform powdered additive.

3. The method for preparing a sheep-strengthening powder according to claim 2, characterized in that: Before selecting the raw materials, the raw materials need to be pre-treated in the first step. The specific steps are as follows: Select fresh purslane, rinse with clean water to remove impurities, dry at a low temperature of 60℃ until the moisture content is ≤8%, pulverize and pass through an 80-mesh sieve to obtain purslane dry powder. Centella asiatica extract and Codonopsis pilosula extract were purified by high-performance liquid chromatography (HPLC) to ensure the absence of heavy metal residues; beneficial bacteria were inoculated into sterile culture medium and incubated at 37°C for 12 hours, with the activated bacterial concentration ≥ CFU / mL, for later use.

4. The method for preparing a sheep-strengthening powder according to claim 2, characterized in that: During the solid component mixing process in step two, the rotation speed of the twin-helix mixer is controlled at 200-300 r / min.

5. The method for preparing a sheep-strengthening powder according to claim 2, characterized in that: In step three, after the bacterial solution is dispersed, the material humidity is controlled to be ≤15% to prevent the live bacteria from becoming moldy.

6. The method for preparing a sheep-strengthening powder according to claim 2, characterized in that: In step four, the temperature for vacuum freeze drying after fermentation is -40℃, and the vacuum degree is 0.06-0.08MPa.

7. The method for preparing a sheep-strengthening powder according to claim 2, characterized in that: After the powdered additive is prepared, it needs to be subjected to quality testing. The testing indicators are as follows: total viable bacteria count ≥ CFU / g, asiaticoside ≥0.8%, Codonopsis pilosula polysaccharide ≥1.5%, moisture ≤6%, total mold count ≤ CFU / g.

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