Traditional Chinese medicine compound low-temperature fermentation selenium-enriched nutrient solution, its preparation method and application

Abstract

This invention provides a low-temperature fermentation of traditional Chinese medicine into a selenium-enriched nutrient solution, its preparation method, and its application. It relates to the field of agricultural planting technology. The fermentation substrate used in this application comprises selenium-enriched wheatgrass, wasabi rhizome, astragalus, ginseng, American ginseng, codonopsis, salvia miltiorrhiza, hawthorn, licorice, rhodiola rosea, and asparagus. Wheatgrass (selenium-enriched) and wasabi (selenium-enriched) are the main substrates to ensure stable selenium content in the fermentation liquid and facilitate absorption and conversion. The combination of wasabi and hawthorn inhibits unwanted bacteria and promotes the growth of beneficial bacteria. The combination of ginseng, American ginseng, codonopsis, and astragalus stimulates cell division, thereby promoting root absorption of organic selenium. The phenolic acids and flavonoids abundant in salvia miltiorrhiza and rhodiola rosea stabilize the various nutrients and their effects in the fermentation liquid. Asparagus increases the diversity of amino acids and vitamins in the fermentation liquid, enriching its nutritional components. All components synergistically promote the absorption of total selenium and organic selenium.

Description

Technical Field

[0001] This invention relates to the field of agricultural planting technology, and in particular to a compound low-temperature fermented selenium-enriched nutrient solution of traditional Chinese medicinal herbs, its preparation method, and its application. Background Technology

[0002] Selenium is an essential trace element for the human body, as identified by the World Health Organization. Its basic functions include antioxidation, eliminating free radicals, and participating in metabolism. Therefore, selenium plays a very important and unique role in improving immunity, delaying aging, promoting metabolism, antagonizing toxins, and helping to prevent various human diseases.

[0003] Selenium-enriched nutrient solution is currently the primary material used for the effective and safe production of selenium-enriched products. Existing methods for preparing selenium-enriched nutrient solutions primarily use selenium-rich plants such as violets and watercress, or raw materials such as selenium yeast, sodium selenite (inorganic selenium), inorganic salts, and proteases, fermented for 1-3 days under ambient or high-temperature conditions. The main drawbacks are as follows:

[0004] 1. The fermentation cycle is short, the selenium content of the nutrient solution is low, and there is a high risk of contamination by miscellaneous bacteria.

[0005] 2. The conversion rate of organic selenium is generally low, ranging from 50% to 85%, posing a risk of chemical toxicity.

[0006] 3. High fermentation temperature leads to significant loss of heat-sensitive active substances.

[0007] 4. It has a single function and lacks a multi-raw material synergistic effect system.

[0008] 5. Low bioavailability. Summary of the Invention

[0009] In order to provide a selenium-enriched nutrient solution with high organic selenium content and high bioavailability conversion rate, this application provides a low-temperature fermentation selenium-enriched nutrient solution of traditional Chinese medicine, its preparation method and application.

[0010] In a first aspect, this application provides a fermentation substrate for a low-temperature fermentation of traditional Chinese medicine compound nutrient solution rich in organic selenium, comprising, by weight:

[0011] 30-50 parts of selenium-enriched substrate: total selenium content 55-130 mg / kg, organic selenium content ≥ 80%; the selenium-enriched substrate includes any one or more of selenium-enriched wheat seedlings, selenium-enriched wasabi fleshy rhizomes, and highland barley seedlings;

[0012] It contains 45-70 parts of saponins and cell division-promoting substances, including any one or more of ginseng, American ginseng, codonopsis, and astragalus.

[0013] 25-38 parts of substances with natural antioxidant components: including one or more of danshen, safflower, and licorice;

[0014] 10-15 servings of hawthorn;

[0015] 10 portions of Asparagus cochinchinensis;

[0016] Fermentation aids: 10-15 parts red yeast rice and 20-30 parts brown sugar;

[0017] 375-570 portions of water.

[0018] Furthermore, the selenium-enriched substrate consists of 10-20 parts of selenium-enriched wheat seedlings and 20-30 parts of selenium-enriched wasabi fleshy rhizomes;

[0019] Furthermore, the saponin-like substances contained therein that promote cell division are 15-25 parts of Astragalus membranaceus, 10-15 parts of Ginseng, 10-15 parts of American ginseng, and 10-15 parts of Codonopsis pilosula.

[0020] Furthermore, the substance containing natural antioxidants is 10-15 parts of Salvia miltiorrhiza, 10-15 parts of Rhodiola rosea, and 5-8 parts of Glycyrrhiza uralensis.

[0021] Furthermore, the substance containing natural antioxidants is 10-15 parts of Salvia miltiorrhiza, 10-15 parts of Rhodiola rosea, and 5-8 parts of Glycyrrhiza uralensis.

[0022] Secondly, this application provides a method for preparing a low-temperature fermented organic selenium-rich nutrient solution of traditional Chinese medicine, including enzymatic hydrolysis and fermentation steps;

[0023] The fermentation substrate described in this application is used;

[0024] Enzymatic hydrolysis: Place the fermentation substrates other than hawthorn, asparagus, red yeast rice and brown sugar in a fermentation tank, add 0.01-0.3 wt% of compound enzyme preparation, and enzymatically hydrolyze for 3-5 hours at 40℃-48℃ and pH 5.0-5.5.

[0025] Fermentation: Then add the remaining substrates and yeast at a concentration of 1-3 wt% of the fermentation substrates, stir, and seal for fermentation; after fermentation is complete, filter out the fermentation supernatant to obtain a compound low-temperature fermented organic selenium-rich nutrient solution of traditional Chinese medicine.

[0026] Furthermore, the compound enzyme preparation is a composition of cellulase, pectinase and protease in a mass ratio of 7-10.8:3-6.48:2-4.32.

[0027] Furthermore, the fermentation conditions are as follows: sealed fermentation at 0-10℃ for 60-70 days.

[0028] Thirdly, this application provides a compound low-temperature fermentation organic selenium-rich nutrient solution of traditional Chinese medicine, which is obtained by the fermentation substrate described in this application or by the preparation method of compound low-temperature fermentation organic selenium-rich nutrient solution of traditional Chinese medicine.

[0029] Fourthly, this application provides an application of the herbal compound low-temperature fermentation organic selenium-rich nutrient solution as described in this application in the cultivation or planting of wheat crops, rice, vegetables and fruits.

[0030] Selenium-enriched crops can be obtained by spraying with a 1200-1800 times diluted organic selenium nutrient solution or by watering with a 400-600 times diluted solution 2-5 times.

[0031] Beneficial effects: 1. The fermentation substrate used in this application includes selenium-enriched wheatgrass, wasabi fleshy rhizomes, astragalus, ginseng, American ginseng, codonopsis, salvia miltiorrhiza, hawthorn, licorice, rhodiola rosea, asparagus, red yeast rice, and brown sugar. Wheatgrass (selenium-enriched) and wasabi (selenium-enriched) are the main substrates to achieve stable and controllable selenium content in the fermentation liquid, making it easier for plants to absorb and convert (after multiple conversions by wheatgrass and wasabi, not only is the organic selenium content increased, but the organic selenium is also more easily absorbed by plants). The combination of wasabi (which has isothiocyanates with broad-spectrum antibacterial and insecticidal activity) and hawthorn (rich in organic acids) can regulate the pH value of the fermentation system, inhibit miscellaneous bacteria, and promote the growth of beneficial bacteria. The combination of ginseng, American ginseng, codonopsis, and astragalus contains saponins, which have effects similar to auxins and cytokinins, stimulating cell division, promoting root development, resulting in more developed roots and thicker stems, and further promoting the absorption of organic selenium by the roots. Salvia miltiorrhiza and Rhodiola rosea are rich in phenolic acids (such as salvianolic acid) and flavonoids (such as rhodioloside derivatives), which are powerful natural antioxidants that can stabilize the various nutrients and effects in the fermentation liquid. Asparagus cochinchinensis is rich in amino acids, vitamins (especially folic acid), rutin, and asparagine, increasing the diversity of amino acids and vitamins in the fermentation liquid and enriching its nutritional components.

[0032] 2. The nutrient solution of this application has a high and relatively stable total selenium content and organic selenium content. Microbial activity is slowed down under refrigeration, which can inhibit contamination by miscellaneous bacteria during fermentation. It has a high absorption and utilization rate in planting and production applications and takes effect quickly after application. After spraying with a 1200-1800 times diluted solution and watering with a 500 times diluted solution 2-3 times, the selenium content can reach a level far exceeding the industry standard for selenium-enriched agricultural products.

[0033] 3. The nutrient solution of this application has more comprehensive efficacy, retains the active ingredients of Chinese herbal medicines, and is rich in a variety of biostimulants such as polysaccharides, oligopeptides, amino acids, and organic acids. It has multiple functions such as anti-oxidation, enhancing crop stress resistance, improving nutrient absorption and utilization efficiency, and accelerating plant growth (small molecule nitrogen-containing substances can promote rapid plant growth as signaling substances), thereby promoting the absorption and conversion of organic selenium.

[0034] 4. Through comparative experiments, the types and ratios of fermentation substrates are screened to achieve higher plant absorption and utilization rates and richer nutritional value. Low-temperature, long-term sealed fermentation reduces the loss of heat-sensitive active substances and forms organic selenium that is more easily absorbed and utilized by plants. It also avoids contamination by miscellaneous bacteria and helps to obtain a compound fermentation nutrient solution with small molecules, easy absorption, high activity, and fewer side effects. The efficient conversion of inorganic selenium into safe and highly active organic selenium reduces the risk of chemical toxicity and improves plant absorption and utilization rates. Detailed Implementation

[0035] To make the technical solution of the present invention clearer, the present invention will be further described in detail below with reference to specific embodiments.

[0036] Preparation Example 1: A method for producing high-organic selenium plants, seedling powder and seeds, including a first cultivation cycle, a second cultivation cycle, a third cultivation cycle and a fourth cultivation cycle.

[0037] Barley seeds and wasabi seeds and cultivation bases: Commercially available barley seeds were planted in agricultural production bases in Shigatse, Tibet, and commercially available wasabi seeds were planted in agricultural production bases in the western mountainous areas of Sichuan. The fermented nutrient solution of this application was used as irrigation in the furrows before planting, and the fermented nutrient solution of this application was sprayed as foliar fertilizer during the flowering period and the early stage of grain filling. The rest of the planting was carried out in accordance with normal planting methods.

[0038] First breeding cycle:

[0039] The raw materials for the fermentation nutrient solution I used include the fermentation substrate and 1500 mg / kg of yeast selenium (selenium content 2 g / kg, which may be 1-3 g / kg in other embodiments); where parts are by weight.

[0040] Fermentation substrate: 15 parts of commercially available common wheatgrass, 25 parts of commercially available common wasabi fleshy root, 20 parts of astragalus, 12 parts of ginseng, 12 parts of American ginseng, 13 parts of codonopsis, 12 parts of salvia miltiorrhiza, 12 parts of rhodiola rosea, 12 parts of hawthorn, 5 parts of licorice, 10 parts of asparagus, 10 parts of red yeast rice, 25 parts of brown sugar, and 500 parts of water.

[0041] In this embodiment, the commercially available common wheat seedlings used have a selenium content of 30 mg / kg, with organic selenium accounting for 85 wt% of the total selenium; generally, commercially available common wheat seedlings have a selenium content of 30-60 mg / kg. The commercially available common wasabi fleshy roots used in this embodiment have a selenium content of 0.3 mg / kg, with organic selenium accounting for 80 wt% of the total selenium; generally, commercially available selenium-enriched wasabi roots and stems have a selenium content of 0.3-2 mg / kg.

[0042] Fermentation: All fermentation substrates except hawthorn, asparagus, red yeast rice, and brown sugar were placed in a fermenter. A complex enzyme preparation consisting of cellulase, pectinase, and protease at a mass ratio of 5:3:2 (0.1 wt% of the fermentation substrate) was added, and enzymatic hydrolysis was carried out at 45 ℃ and pH 5.0 for 4 hours. Then, the remaining substrates (hawthorn, asparagus, red yeast rice, and brown sugar), yeast selenium, and yeast (2 wt% of the fermentation substrate) were added, stirred, and fermented in a sealed environment at 9 ℃ for 65 days. The fermentation supernatant was filtered out to obtain fermentation nutrient solution I.

[0043] Barley cultivation:

[0044] Pre-planting furrow irrigation: Dilute fermented nutrient solution I 500 times, and use 20 cubic meters of water per acre per application.

[0045] Foliar fertilizer (fermented nutrient solution I): During the flowering and early grain-filling stages, dilute fermented nutrient solution I 1500 times and spray; use 200 liters per acre each time, ensuring even spraying on both sides of the leaves, avoiding dripping. Choose a windless, sunny afternoon after 4 pm for spraying.

[0046] Wasabi cultivation:

[0047] Pre-planting furrow irrigation: Dilute fermented nutrient solution I 500 times. Use 15 cubic meters of water per acre per application.

[0048] Wasabi is sown in mid-October for seedling cultivation. After 4-5 months, when the seedlings have 3-4 leaves and are 10-15cm tall, they are transplanted. After transplanting, shading management should be carried out. In spring and autumn, the roots should be irrigated twice with 500× fermented nutrient solution (with an interval of no less than 15 days between the two irrigations). When irrigating the roots, the roots should be 5-10cm away from the roots. The rhizomes can be harvested 15 months after transplanting. They are selenium-rich wasabi fleshy rhizomes. During this period, the leaves and petioles can be harvested twice, and the flower stalks can be harvested once.

[0049] After the plant matures, its seeds are collected; crop cultivation consists of a cultivation cycle from land irrigation to seed harvest. The selenium content and inorganic selenium content in plant seeds (roots and rhizomes) are measured, and then the organic selenium content is calculated.

[0050] Preparation of wheat seedlings:

[0051] Using the barley seeds obtained from the above-mentioned planting (obtained in the first cultivation cycle), soak them in 40℃ warm water for 24 hours. After the seeds show signs of sprouting, spread them evenly at 860g / tray onto 60cm×30cm seedling trays, and place them on 4-layer seedling racks (layer spacing 40cm-50cm). Cultivate for 15 days in a controlled environment of 18℃-25℃, 8000lx-10000lx, and 85%-90%RH. During this period, every 8 hours, spray a diluted fermented nutrient solution I at a certain ratio using atomized spraying. From day 1 to day 5 after placing on the racks, spray with 800× fermented nutrient solution using atomized spraying; from day 5 to day 8, spray with 500× fermented nutrient solution using atomized spraying; after day 8, spray with 350× fermented nutrient solution using atomized spraying. The amount of spraying each time should be such that water droplets condense on the seeds or leaves. During the cultivation period, change the position of the seedling trays every 2-3 days, alternating between vertical and horizontal positions.

[0052] When the seedlings reach a height of 15-20 cm, the entire plant is washed and dried in a drying room at 50-60℃ for 20 hours. The seeds and leaves are then separated. The leaf portion is pulverized into dry powder using a cell-level vibration ultrafine pulverizer to obtain wheat seedlings. The selenium content and organic selenium content in the wheat seedlings are then tested.

[0053] In other embodiments, optional substrates include: 10-20 parts of commercially available ordinary wheatgrass seedling powder or wheatgrass obtained using the method of this application; 20-30 parts of commercially available ordinary selenium-enriched wasabi rhizome or selenium-enriched wasabi rhizome obtained using the method of this application; 15-25 parts of Astragalus membranaceus; 10-15 parts of ginseng; 10-15 parts of American ginseng; 10-15 parts of Codonopsis pilosula; 10-15 parts of Salvia miltiorrhiza; 10-15 parts of Rhodiola rosea; 10-15 parts of hawthorn; 5-8 parts of licorice; 10 parts of Asparagus cochinchinensis; 10-15 parts of red yeast rice; and 20-30 parts of brown sugar.

[0054] In other embodiments, the fermentation substrate includes: 10-20 parts wheat seedlings, 20-30 parts selenium-enriched wasabi rhizome, 15-25 parts astragalus, 10-15 parts ginseng, 10-15 parts American ginseng, 10-15 parts codonopsis, 10-15 parts salvia miltiorrhiza, 10-15 parts rhodiola rosea, 5-8 parts licorice, 10-15 parts hawthorn, 10 parts asparagus, 10-15 parts red yeast rice, and 20-30 parts brown sugar.

[0055] In other embodiments, fermentation was carried out as follows: The fermentation substrates, excluding hawthorn, asparagus, red yeast rice, and brown sugar, were placed in a fermenter. A compound enzyme preparation of cellulase, pectinase, and protease at a mass ratio of 7-10.8:4.2-6.48:2.8-4.32 (the compound enzyme preparation accounted for 0.1-0.3 wt% of the fermentation substrate) was added, and enzymatic hydrolysis was performed for 3-5 hours at 40℃-48℃ and pH 5.0-5.5. Then, the remaining substrates (hawthorn, asparagus, red yeast rice, and brown sugar), yeast selenium, and yeast (1-3 wt% of the fermentation substrate) were added, stirred, and fermented in a sealed environment at no higher than 10℃ for 60-70 days. The fermentation supernatant was filtered out to obtain the fermentation nutrient solution.

[0056] Second breeding cycle:

[0057] The difference between the second cultivation cycle and the first cultivation cycle is that fermentation nutrient solution II is used to replace fermentation nutrient solution I in equal amounts; and the seeds used for planting are barley seeds and wasabi seeds obtained in the second cultivation cycle.

[0058] The difference between fermentation nutrient solution II and fermentation nutrient solution I is as follows: ① wheat seedlings obtained in the first cultivation cycle are used to replace ordinary wheat seedlings in equal amounts; ② wasabi rhizomes obtained in the first cultivation cycle are used to replace ordinary wasabi rhizomes in equal amounts; ③ the amount of yeast selenium used is half that in fermentation nutrient solution I.

[0059] Third breeding cycle:

[0060] The difference between the third cultivation cycle and the first cultivation cycle is that fermentation nutrient solution III is used to replace fermentation nutrient solution I in equal amounts; and the seeds used for planting are barley seeds and wasabi seeds obtained in the second cultivation cycle.

[0061] The difference between fermentation nutrient solution III and fermentation nutrient solution I is as follows: ① wheat seedlings obtained in the second cultivation cycle are used to replace ordinary wheat seedlings in equal amounts; ② wasabi rhizomes obtained in the second cultivation cycle are used to replace ordinary wasabi rhizomes in equal amounts; ③ yeast selenium is not used.

[0062] Fourth breeding cycle:

[0063] The difference between the fourth cultivation cycle and the first cultivation cycle is that fermentation nutrient solution IV is used to replace fermentation nutrient solution I in equal amounts; and the seeds used for planting are barley seeds and wasabi seeds obtained in the third cultivation cycle.

[0064] The difference between fermented nutrient solution IV and fermented nutrient solution I is as follows: ① seedlings obtained in the third cultivation cycle are used to replace ordinary wheat seedlings in equal amounts; ② wasabi rhizomes obtained in the third cultivation cycle are used to replace ordinary wasabi rhizomes in equal amounts; ③ yeast selenium is not used.

[0065] Table 1 lists the selenium content in seeds and seedling powder obtained from the first to the fourth cultivation cycle. Each test data n=10 (10 samples are randomly selected for testing, and 3 values ​​are taken from each sample, and the average value is taken). a represents the total selenium content in mg / kg; b represents the organic selenium content in mg / kg.

[0066] Total selenium (mg / kg) was determined using the first method shown in GB5009.93-2017; inorganic selenium content was determined using the first method shown in DBS42 / 010-2018. Organic selenium content (mg / kg) was obtained by subtracting inorganic selenium content from total selenium content.

[0067] Table 1 lists the selenium content in seeds and seedling powder obtained from the first to the fourth cultivation cycles.

[0068]

[0069] Example 1: A method for preparing a low-temperature fermentation nutrient solution rich in organic selenium from traditional Chinese medicine:

[0070] Fermentation substrate: crushed selenium-enriched wheat seedlings (using the third cultivation cycle of Preparation Example 1), crushed wasabi fleshy rhizomes (using the third cultivation cycle of Preparation Example 1), astragalus, ginseng, American ginseng, codonopsis, salvia miltiorrhiza, hawthorn, licorice, rhodiola rosea, asparagus, red yeast rice, brown sugar (brown sugar and black sugar in a 1:1 mass ratio) and water.

[0071] Fermentation: All fermentation substrates except hawthorn, asparagus, red yeast rice, and brown sugar were placed in a fermentation tank. A complex enzyme preparation consisting of cellulase, pectinase, and protease in a mass ratio of 5:3:2 was added, and enzymatic hydrolysis was carried out at 45℃ and pH 5.0 for 4 hours. Then, the remaining substrates (hawthorn, asparagus, red yeast rice, and brown sugar) and yeast (2 wt% of the fermentation substrates) were added, stirred, and fermented in a sealed environment at 9℃ for 65 days. The fermentation supernatant was filtered out to obtain a compound low-temperature fermented organic selenium-rich nutrient solution of traditional Chinese medicine.

[0072] The difference between Examples 2 and 3 and Example 1 is that the types and weights of the raw material substrates used are different, as shown in Table 2.

[0073] Table 2, List of types and weight settings of raw material substrates used in Examples 1 to 3

[0074]

[0075] Example 4: A method for preparing a compound low-temperature fermented organic selenium-rich nutrient solution of traditional Chinese medicine, which differs from Example 1 in that the fermentation is carried out at 25°C for 60 days.

[0076] Comparative Example 1: A method for preparing a compound low-temperature fermented organic selenium-rich nutrient solution of traditional Chinese medicine, which differs from Example 1 in that ginseng, American ginseng and codonopsis are not used, and selenium-enriched wheatgrass is used in equal amounts to replace ginseng, American ginseng and codonopsis.

[0077] Comparative Example 2: A method for preparing a compound low-temperature fermented organic selenium-rich nutrient solution of traditional Chinese medicine, which differs from Example 1 in that it does not use danshen, safflower and licorice, but uses selenium-rich wheat seedlings in equal amounts to replace danshen, safflower and licorice.

[0078] Comparative Example 3: A method for preparing a compound low-temperature fermented organic selenium-rich nutrient solution of traditional Chinese medicine, which differs from Example 1 in that it does not use asparagus, but uses selenium-rich wheat seedlings in an equal amount to replace asparagus.

[0079] Comparative Example 4: A method for preparing a compound low-temperature fermented organic selenium-rich nutrient solution of traditional Chinese medicine, which differs from Example 1 in that it does not use selenium-enriched wheat seedlings and wasabi fleshy rhizomes; and adds appropriate amounts of sodium selenite (inorganic selenium) and yeast selenium (organic selenium) to adjust the organic selenium and total selenium to be the same as in Example 1.

[0080] Application Example 1. In March-April 2025, at the Shulan Agricultural Production Base in Hangzhou, organic selenium-rich nutrient solutions (using the organic selenium nutrient solutions of Examples 1-4 and Comparative Examples 1-4 respectively) were diluted 1500 times with water and sprayed on the leaves of *Gnaphalium affine* 15 days before harvest (230 liters per mu) and 7 days before harvest (260 liters per mu). After harvest, the total selenium and organic selenium content were tested using the first method shown in GB5009.93-2017. The results are shown in Table 3.

[0081] Application Example 2. In March-April 2025, organic selenium-rich nutrient solution (using the organic selenium nutrient solution of Example 1) was diluted 1300 times with water at the Shulan Agricultural Production Base in Hangzhou. It was sprayed on the leaves of fruit cucumbers 15 days before harvest (100 liters per mu) and 7 days before harvest (130 liters per square meter). After harvest, the total selenium content was tested using the first method shown in GB5009.93-2017. The total selenium content was 0.01 mg / kg, and the organic selenium content was 98%.

[0082] Application Example 3. From May to June 2025, at the Shulan Agricultural Production Base in Hangzhou, organic selenium-rich nutrient solutions were applied to the roots of fruiting peach trees twice (5 liters / tree) and once (200 liters / acre) using organic selenium-rich nutrient solutions from Examples 1-4 and Comparative Examples 1-4, diluted 500 times with water. The harvested peaches were tested using the first method shown in GB5009.93-2017, and the total selenium and organic selenium content are shown in Table 3.

[0083] Application Example 4. From May to July 2025, at the Hangzhou Dingxin Agricultural Production Base, an appropriate amount of selenium-enriched nutrient solution (using the organic selenium nutrient solution from Example 1) was evenly mixed into the *Pleurotus ostreatus* substrate (1500ml of selenium-enriched nutrient solution diluted 500 times with water per substrate tank), forming 1260 mycelium logs. After inoculation, mycelial growth, fruiting, and harvesting, 310 kg of fresh mushrooms were obtained. Detection using Method 1 as shown in GB5009.93-2017 showed a total selenium content of 3.18 mg / kg and an organic selenium content of 98%.

[0084] Application Example 5. From September to October 2025, at the Wuwangnong production base in Zhejiang, single-season rice was sprayed with organic selenium-rich nutrient solution using the organic selenium nutrient solutions of Examples 1-4 and Comparative Examples 1-4, diluted 1200 times with water (150 liters per mu). After harvesting, the total selenium and organic selenium content were tested using the first method shown in GB5009.93-2017, as shown in Table 3.

[0085] a represents the selenium content in mg / kg; b represents the content of organic selenium in total selenium in mg / kg.

[0086] Table 3. Content of total selenium and organic selenium in *Boletus lucidum*, peach, and rice using different selenium-enriched nutrient solutions.

[0087]

[0088] The embodiments described above are merely illustrative of several implementations of the present invention, and while the descriptions are specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these modifications and improvements all fall within the scope of protection of the present invention. Therefore, the scope of protection of this patent should be determined by the appended claims.

Claims

1. A fermentation substrate for a low-temperature fermentation of traditional Chinese medicine compound containing selenium-rich organic nutrients, characterized in that, By weight, it includes: 30-50 parts of selenium-enriched substrate: total selenium content 55-130 mg / kg, organic selenium content ≥ 80%; the selenium-enriched substrate includes any one or more of selenium-enriched wheat seedlings, selenium-enriched wasabi fleshy rhizomes, and highland barley seedlings. It contains 45-70 parts of saponins and cell division-promoting substances, including any one or more of ginseng, American ginseng, codonopsis, and astragalus. 25-38 parts of substances with natural antioxidant components: including one or more of danshen, safflower, and licorice; 10-15 servings of hawthorn; 10 portions of Asparagus cochinchinensis; Fermentation aids: 10-15 parts red yeast rice and 20-30 parts brown sugar; 375-570 portions of water.

2. The fermentation substrate for a low-temperature fermentation of traditional Chinese medicine compound rich in organic selenium nutrient solution according to claim 1, characterized in that, The selenium-enriched substrate consists of 10-20 parts of selenium-enriched wheat seedlings and 20-30 parts of wasabi fleshy rhizomes.

3. The fermentation substrate for a low-temperature fermentation of traditional Chinese medicine compound rich in organic selenium nutrient solution according to claim 1 or 2, characterized in that, The saponins contained therein that promote cell division are 15-25 parts of Astragalus membranaceus, 10-15 parts of Ginseng, 10-15 parts of American ginseng, and 10-15 parts of Codonopsis pilosula.

4. The fermentation substrate for a low-temperature fermentation of traditional Chinese medicine compound rich in organic selenium nutrient solution according to claim 3, characterized in that, The substances containing natural antioxidants are 10-15 parts of Salvia miltiorrhiza, 10-15 parts of Rhodiola rosea, and 5-8 parts of Glycyrrhiza uralensis.

5. The fermentation substrate for a low-temperature fermentation solution of traditional Chinese medicine rich in organic selenium according to any one of claims 1-2 and 4, characterized in that, The substances containing natural antioxidants are 10-15 parts of Salvia miltiorrhiza, 10-15 parts of Rhodiola rosea, and 5-8 parts of Glycyrrhiza uralensis.

6. A method for preparing a low-temperature fermentation nutrient solution rich in organic selenium from traditional Chinese medicine, characterized in that, Includes enzymatic hydrolysis and fermentation steps; The fermentation substrate described in any one of claims 1-5 is used; Enzymatic hydrolysis: Place the above-mentioned fermentation substrates, excluding hawthorn, asparagus, red yeast rice, and brown sugar, into a fermentation tank, add 0.1-0.3 wt% of a compound enzyme preparation, and enzymatically hydrolyze for 3-5 hours at 40℃-48℃ and pH 5.0-5.

5. Fermentation: Then add the remaining substrates and yeast at a concentration of 1-3 wt% of the fermentation substrates, stir, and seal for fermentation; after fermentation is complete, filter out the fermentation supernatant to obtain a compound low-temperature fermented organic selenium-rich nutrient solution of traditional Chinese medicine.

7. The method for preparing a low-temperature fermentation organic selenium-rich nutrient solution of traditional Chinese medicine according to claim 6, characterized in that, The compound enzyme preparation is a composition of cellulase, pectinase and protease in a mass ratio of 7-10.8:4.2-6.48:2.8-4.

32.

8. A method for preparing a low-temperature fermentation organic selenium-rich nutrient solution of traditional Chinese medicine according to claim 6 or 7, characterized in that, The fermentation conditions are as follows: sealed fermentation at 0-10℃ for 60-70 days.

9. A compound low-temperature fermented nutrient solution rich in organic selenium from traditional Chinese medicine, characterized in that, It comprises the fermentation substrate according to any one of claims 1-5 or is obtained by the preparation method according to any one of claims 6-8.

10. The application of the herbal compound low-temperature fermentation organic selenium-rich nutrient solution as described in claim 9 in the cultivation or planting of wheat crops, rice, vegetables and fruits.

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