Method and kit for early warning of milk spoilage

By employing an evaluation strategy that combines endogenous reference materials and response markers, and utilizing the ratio of milk phospholipid metabolism markers to determine milk spoilage risk, this approach solves the problems of time-consuming detection and temperature fluctuation sensitivity in existing milk freshness evaluation technologies, enabling early warning and robust detection.

CN122084785APending Publication Date: 2026-05-26CHINESE ACAD OF INSPECTION & QUARANTINE
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHINESE ACAD OF INSPECTION & QUARANTINE
Filing Date
2026-02-12
Publication Date
2026-05-26

AI Technical Summary

Technical Problem

Existing methods for evaluating milk freshness are time-consuming, insensitive to temperature fluctuations, and difficult to achieve dynamic early warning of the supply chain. Furthermore, absolute quantitative methods based on single compounds are easily affected by sample differences and matrix effects, and lack threshold universality and field deployment.

Method used

An evaluation strategy based on endogenous reference and response markers was adopted. After extraction, precipitation, and centrifugation, chromatography-mass spectrometry analysis was performed. The ratio of milk phospholipid metabolism markers such as LPE (16:0) and PC (16:0/18:1) was used to determine the risk of milk spoilage, and a spoilage coefficient R was established for early warning.

Benefits of technology

It achieves a sensitive response to quality changes caused by temperature abuse, and can provide early warnings before traditional endpoint indicators show significant changes. It is suitable for spoilage early warning detection of milk from different origins and batches, and has the advantages of robustness and easy judgment, making it suitable for circulation monitoring scenarios.

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Abstract

This invention provides a method and kit for early warning of milk spoilage. The method includes: mixing the milk to be tested with an extract and performing ultrasonic extraction to obtain an extract; precipitating the extract to obtain a precipitated solution; centrifuging the precipitated solution to obtain a supernatant; analyzing the spoilage markers in the supernatant using chromatography-mass spectrometry to obtain detection values; and confirming whether the milk has a risk of spoilage based on the detection values. This method utilizes spoilage markers for judgment, can sensitively respond to quality changes caused by temperature abuse, and can provide early warning before significant changes occur in traditional endpoint indicators. It is applicable to spoilage early warning detection of milk from different origins and batches, and has the advantages of robustness and ease of judgment. It is also suitable for freshness evaluation indicators in circulation monitoring scenarios.
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