A fruit fly disorienting agent, its preparation method and application

Abstract

The present application belongs to the technical field of fruit fly prevention and control, and discloses a disorienting agent, a preparation method and application thereof. The disorienting agent contains rosemary extract and orange peel essential oil. The rosemary extract and orange peel essential oil contain terpenes and other compounds (such as 1,8-cineole in rosemary), which can compete with natural signal molecules to bind to odor binding proteins or olfactory receptors, which can cause fruit flies to fail to activate the correct nerve signal or directly block the signal transduction pathway. The orange peel essential oil in the disorienting agent has a strong smell, which can strongly cover or mask the weak smell signal emitted by the fruit, forming an "olfactory masking" effect, so that the fruit fly cannot find the fruit. Moreover, compared with sex pheromones as disorienting agents, which can only act on male fruit flies, the disorienting agent of the present application can interfere with the olfactory receptors of adult fruit flies to make them unable to find the target, so that they cannot accurately locate the oviposition site and harm the fruit. The disorienting agent is effective for both female and male fruit flies.

Description

Technical Field

[0001] This invention relates to the field of fruit fly technology, and more specifically, to a fruit fly mating agent, its preparation method, and its application. Background Technology

[0002] Fruit fly demagnetizers are green and effective pest control agents. They release high concentrations of fruit fly species-specific sex pheromones in the field, masking the natural sex pheromone signals released by females and desensitizing the olfactory receptors of males. This blocks mating between male and female adults, reducing the number of offspring, hindering fruit flies from foraging for and laying eggs on host plants, and effectively suppressing fruit fly damage.

[0003] Fruit fly mating agents are composed primarily of fruit fly species-specific pheromones as their core active ingredients. Common examples include methyl eugenol (corresponding to the pheromone of the oriental fruit fly), cis-9-tricarne and cis-11-tricarne (corresponding to the oriental fruit fly), and insecticidal ether analogs (corresponding to the Mediterranean fruit fly). These can effectively reduce the rate of infested fruit in orchards. However, existing fruit fly mating agents have the following problems: (1) The disorientation effect on fruit flies is poor, and it cannot effectively protect fruit in orchards. The core function of the active component of the disorientation agent, sex pheromone, is to target the sexual olfactory recognition system of male fruit fly adults. However, sex pheromones do not act on the general olfactory pathways of fruit flies to recognize fruit, and cannot interfere with the nerve signal transmission of fruit flies to locate fruit. Even if male adults are interfered with by sex pheromones, female and male adults can still recognize the smell of fruit and accurately locate fruit through the normal olfactory system. Moreover, the intensity of the sex pheromone smell is insufficient to mask the smell of fruit, and it cannot form an "olfactory masking" effect.

[0004] (2) The range of action is narrow, only interfering with male fruit flies, but not with female fruit flies. Fruit fly mating agents are based on sex pheromones, and their mechanism of action targets only the male adult's olfactory recognition system. They can only attract or interfere with male adults, but cannot affect the olfactory perception function of female adults. Female adults can normally locate the fruit and lay eggs through their sense of smell, so even if the male adults are disturbed, the fruit will still be damaged by the oviposition behavior of the female adults. Summary of the Invention

[0005] This invention provides a fruit fly demagnetizer that can effectively interfere with the universal olfactory receptors of male and female fruit flies, and its demagnetizing effect is superior to that of the prior art.

[0006] Another object of the present invention is to provide a method for preparing the fruit fly mating agent.

[0007] Another object of the present invention is to provide the application of the fruit fly migratory agent in the control of fruit flies.

[0008] To solve the above-mentioned technical problems, the technical solution provided by the present invention is as follows: A pheromone containing rosemary extract and orange peel essential oil.

[0009] The aphrodisiac component of this invention contains rosemary extract and orange peel essential oil, and has the following technical effects: (1) Excellent disorientation effect on fruit flies, effectively protecting orchard fruits. The general olfactory pathway for fruit flies to identify fruits relies on the binding of odor-binding proteins and olfactory proteins on their antennae to the natural signal molecules emitted by the fruit. The terpenoids and other compounds (such as 1,8-cineole in rosemary) contained in rosemary extract and orange peel essential oil compete with natural signal molecules to bind to odor-binding proteins or olfactory receptors. This can prevent fruit flies from activating the correct neural signals or directly block the signal transmission pathway. Even if some natural signal molecules bind to odor-binding proteins or olfactory receptors, the resulting incorrect or confused neural signals will prevent the fruit fly brain from interpreting the correct navigation information. Moreover, the strong odor of orange peel essential oil in the disorientation agent can effectively mask or block the weak odor signals emitted by the fruit, forming an "olfactory masking" that prevents fruit flies from finding the fruit.

[0010] (2) It has a wide range of effects, not only interfering with male fruit flies, but also with female fruit flies. Compared with sex pheromones, which can only act on male fruit flies as a decoy, the decoy of this invention interferes with the olfactory receptors of adult fruit flies, making them unable to find their target and thus unable to accurately locate and lay eggs to damage the fruit. It is effective for both female and male fruit flies.

[0011] Preferably, the mass fractions of each component of the deodorant are: 3-10 parts rosemary extract and 3-10 parts orange peel essential oil.

[0012] More preferably, the mass fractions of each component of the deodorant are: 4-7 parts rosemary extract and 4-7 parts orange peel essential oil.

[0013] Preferably, the fuming agent also contains Sichuan pepper essential oil and peppermint essential oil, in the following proportions by weight: 1-2 parts Sichuan pepper essential oil and 1-2 parts peppermint essential oil.

[0014] Preferably, the aphrodisiac also contains patchouli essential oil.

[0015] The patchouli alcohol and other substances in patchouli essential oil are highly volatile and have a strong aroma, which can further "mask" the fruit flies, making it impossible for them to find the fruit.

[0016] The patchouli essential oil in the pheromone component of the present invention is 1 to 2 parts by weight.

[0017] Preferably, the fuming agent component also contains Sichuan pepper essential oil and peppermint essential oil.

[0018] The magnifying agent component of the present invention also contains the natural synergist sesamin, in parts by weight of 3 to 5.

[0019] The sesamin in the demagnetizing agent described in this invention is a natural product synergist that can inhibit multiple detoxification enzymes of fruit flies at multiple targets, preventing them from harming the fruit. Moreover, it is a natural and environmentally friendly product.

[0020] The disorienting agent described in this invention, in addition to interfering with the olfactory receptors of fruit flies, also disorients fruit flies through multiple targets and pathways by stimulating nerves and interfering with temperature receptors. Specifically, Sichuan pepper essential oil contains salinomycin, represented by α-salinomycin, which can activate the TRPA1 ion channel on the sensory nerves of fruit flies, triggering continuous neural electrical signals and causing the fruit flies to feel strong discomfort through nerve stimulation. The menthol, nepeta lactone, and other components in peppermint essential oil can activate the TRP family receptors in the fruit fly, especially the TRPA1 channel. Once activated, this channel transmits harmful signals to the fruit fly's nervous system, similar to contact with "overheating," "overcooling," or toxic substances, causing strong discomfort to the fruit flies by interfering with temperature receptors, thus preventing them from finding the fruit.

[0021] In addition, substances with different volatile components in multi-component maze agents can produce a more complex and longer-lasting odor field, thus extending the duration of maze effects.

[0022] The present invention also provides a method for preparing the deflector described in any of the above claims, comprising the following steps: S1. Add a compound emulsifier to the rosemary extract and emulsify it evenly to obtain an emulsion; S2. Slowly add orange peel essential oil to the emulsion, then add a compound emulsifier until the emulsion is uniform, and then add a surfactant and mix evenly to obtain a mascara.

[0023] In the preparation process of the mascara of this invention, the emulsifier is slowly poured into the stirred essential oil in a thin stream. Initially, a W / O type emulsion is formed. As the aqueous phase is continuously added, the system undergoes a "phase inversion" and eventually becomes a more stable O / W type emulsion. This processing method can obtain mascara with smaller particles.

[0024] The key operation is as follows: while mixing, a homogenizer must be used for high-speed shearing for 2-5 minutes until a uniform, fine, milky-white matrix is ​​obtained; the homogenizer speed is 3000-5000 rpm. After obtaining a uniform, fine, milky-white matrix, the speed is switched to slow stirring. After emulsification is complete, slow stirring is continued (to prevent the intake of excessive air bubbles).

[0025] Preferably, the emulsifier in the preparation method of the emulsifier is a composite emulsifier of calcium dodecyl sulfonate and phenylethyl phenol polyoxyethylene ether phosphate in a mass ratio of 1:1.

[0026] The amount of emulsifier added to S1 and S2 in this invention is 5 to 10 parts by weight.

[0027] The present invention also provides a method for controlling fruit flies, using any of the above-described misdirection agents.

[0028] The preferred method for controlling fruit flies is to dilute the attractant 200-500 times and spray it in the orchard. Detailed Implementation

[0029] The present invention will be further described in detail below with reference to specific embodiments.

[0030] Example 1 A pheromone extract containing rosemary extract, orange peel essential oil, Sichuan pepper essential oil, peppermint essential oil, and patchouli essential oil, with the following mass percentages: 5 parts rosemary extract, 5 parts orange peel essential oil, 1.5 parts Sichuan pepper essential oil, 1.5 parts peppermint essential oil, 1.5 parts patchouli essential oil, and 4 parts sesamin, a natural synergist.

[0031] The method for preparing the deflector includes the following steps: S1. Add 5 parts of compound emulsifier to the rosemary extract and emulsify it evenly to obtain an emulsion; S2. Orange peel essential oil is slowly added to the emulsion, then 10 parts of compound emulsifier are added and emulsified evenly, then sesamin is added and mixed evenly to obtain a mascara; the emulsifier is a compound emulsifier with a mass ratio of calcium dodecyl sulfonate and phenylethylphenol polyoxyethylene ether phosphate of 1:1.

[0032] In the preparation process of the mascara of this invention, the emulsifier is slowly poured into the stirred essential oil in a thin stream. Initially, a W / O type emulsion is formed. As the aqueous phase is continuously added, the system undergoes a "phase inversion" and eventually becomes a more stable O / W type emulsion. This processing method can obtain mascara with smaller particles.

[0033] The key operation is as follows: while mixing, a homogenizer must be used for high-speed shearing for 2-5 minutes until a uniform, fine, milky-white matrix is ​​obtained; the homogenizer speed is 3000-5000 rpm. After obtaining a uniform, fine, milky-white matrix, the speed is switched to slow stirring. After emulsification is complete, slow stirring is continued (to prevent the intake of excessive air bubbles).

[0034] Example 2 This embodiment is the second embodiment of the present invention. The difference from embodiment 1 is that the mass parts of each component of the deodorant are: 3 parts of rosemary extract, 10 parts of orange peel essential oil, 1 part of Sichuan pepper essential oil, 2 parts of peppermint essential oil, and 1 part of patchouli essential oil.

[0035] Example 3 This embodiment is the third embodiment of the present invention. The difference from embodiment 1 is that the mass parts of each component of the deodorant are: 10 parts of rosemary extract, 3 parts of orange peel essential oil, 2 parts of Sichuan pepper essential oil, 1 part of peppermint essential oil, and 2 parts of patchouli essential oil.

[0036] Example 4 This embodiment is the fourth embodiment of the present invention. Unlike embodiment 1, the atomizing agent component contains rosemary extract, orange peel essential oil, Sichuan pepper essential oil, and peppermint essential oil, but does not contain patchouli essential oil. The mass parts of each component are: 5 parts of rosemary extract, 5 parts of orange peel essential oil, 1.5 parts of Sichuan pepper essential oil, and 1.5 parts of peppermint essential oil.

[0037] Example 5 This embodiment is the fifth embodiment of the present invention. Unlike embodiment 1, the atomizing agent component contains rosemary extract, orange peel essential oil, and patchouli essential oil, but does not contain Sichuan pepper essential oil and peppermint essential oil. The mass parts of each component are: 5 parts of rosemary extract, 5 parts of orange peel essential oil, and 1.5 parts of patchouli essential oil.

[0038] Example 6 This embodiment is the sixth embodiment of the present invention. Unlike Embodiment 1, the aphrodisiac component contains rosemary extract and orange peel essential oil, but does not contain patchouli essential oil, sesamin, Sichuan pepper essential oil, or peppermint essential oil. The mass parts of each component are: 4 parts rosemary extract and 7 parts orange peel essential oil. Example 7 This embodiment is the 7th embodiment of the present invention. Unlike embodiment 1, the atomizing agent component contains rosemary extract and orange peel essential oil, but does not contain patchouli essential oil, sesamin, Sichuan pepper essential oil and peppermint essential oil. The mass parts of each component are: 4 parts of rosemary extract and 7 parts of orange peel essential oil.

[0039] Comparative Example 1 This comparative example is the first comparative example of the present invention. Unlike Example 6, the demisting agent component does not contain rosemary extract.

[0040] Comparative Example 2 This comparative example is the second comparative example of the present invention. Unlike Example 6, the mascara component does not contain orange peel essential oil.

[0041] Comparative Example 3 This comparative example is the third comparative example of the present invention. The difference from Example 6 is that the rosemary extract in the aphrodisiac component is replaced with the sex pheromone methyleugenol.

[0042] Comparative Example 4 This comparative example is the third comparative example of the present invention. The difference from Example 6 is that the orange peel essential oil is replaced with the sex pheromone methyleugenol. Performance testing: 1. The effect of the mimicry agent on fruit fly mimicry. This invention detects the effect of the mimicry agent on fruit fly mimicry by measuring the fruit infestation rate and fruit retention effect in the field, as detailed below: (1) Setting up the test site Select a uniform orchard severely affected by the oriental fruit fly infestation and divide it into at least two independent areas of no less than one acre each: a mating agent treatment area and a blank control area (without mating agent spraying). The two areas should be at least 100 meters apart to prevent interference. Spray the mating agent evenly in the treatment area.

[0043] (2) Results survey Fruit damage rate survey: During the period when the fruit flies were damaged, 1 to 7 days after the application of the mating agent, a certain number of fruits (no less than 150 in total) were randomly surveyed in the mating agent-treated area and the blank control area using the five-point sampling method.

[0044] Inspect and record the number of damaged fruits with borer holes, sap discharge, or larvae inside.

[0045] (3) Data Analysis The effectiveness of the mimicry agent on fruit flies was evaluated by measuring the fruit infestation rate and fruit retention effect. The following formulas were used to calculate the fruit infestation rate and fruit retention effect, respectively.

[0046] Insect-infested fruit rate % = (Number of infested fruits / Total number of fruits inspected) × 100%; Fruit retention rate % = (1 - Insect-infested fruit rate in the treatment area / Insect-infested fruit rate in the control area) × 100% The results of the test on the disorientation effect of the disorientation agents obtained in Examples 1-7 and Comparative Examples 1-4 on fruit flies are shown in Table 1.

[0047] Table 1 shows the disorientation effects of the disorienting agents obtained from various embodiments and comparative examples.

[0048] 2. The duration of the attraction of the attraction agent to fruit flies. The attraction duration of this invention is evaluated by the duration of effectiveness. The method for evaluating the duration of effectiveness is as follows: The duration of effectiveness of different treatments is evaluated based on the fruit preservation effect investigated daily. When the fruit preservation effect is below 70%, the duration of effectiveness is considered to have ended.

[0049] The test results of the effective duration of the maze agents obtained in Examples 1-7 and Comparative Examples 1-4 are shown in Table 2.

[0050] Table 2 shows the effective duration of the decoys obtained from various embodiments and comparative examples.

[0051] The above description is merely an embodiment of the present invention and does not limit the scope of patent protection. Any non-substantial changes or substitutions made by those skilled in the art based on the present invention will still fall within the scope of patent protection.

Claims

1. A decoy agent, characterized in that, It contains rosemary extract and orange peel essential oil.

2. The decoy agent according to claim 1, characterized in that, The mass fractions of each component are: Rosemary extract 3-10 parts; 3-10 parts orange peel essential oil.

3. The decoy agent according to claim 2, characterized in that, The mass fractions of each component are: Rosemary extract 4-7 parts; 4-7 parts orange peel essential oil.

4. The decoy agent according to claim 2 or 3, characterized in that, It also contains Sichuan pepper oil and peppermint oil, with a weight percentage of: 1-2 parts Sichuan peppercorn essential oil; 1-2 parts peppermint essential oil.

5. The decoy agent according to claim 1, characterized in that, It also contains patchouli essential oil.

6. The decoy agent according to claim 1 or 5, characterized in that, It also contains Sichuan pepper oil and peppermint oil.

7. A method for preparing the disorienting agent according to any one of claims 1 to 6, characterized in that, Includes the following steps: S1. Add a compound emulsifier to the rosemary extract and emulsify it evenly to obtain an emulsion; S2. Slowly add orange peel essential oil to the emulsion, then add a compound emulsifier until the emulsion is uniform, and then add a surfactant and mix evenly to obtain a mascara.

8. The method for preparing the decoy agent according to claim 7, characterized in that, The emulsifier is a composite emulsifier consisting of calcium dodecyl sulfonate and phenylethyl phenol polyoxyethylene ether phosphate in a mass ratio of 1:

1.

9. A method for controlling fruit flies, characterized in that, Use the maze agent as described in any one of claims 1 to 6.

10. The method for controlling fruit flies according to claim 9, characterized in that, Dilute the agitator 200-500 times and spray it in the orchard.

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