A novel antibacterial peptide against listeria monocytogenes and application thereof

Abstract

The application belongs to the technical field of biotechnology, and provides a novel antibacterial peptide against Listeria monocytogenes and application thereof, and an amino acid sequence of the novel antibacterial peptide is shown as SEQ ID NO. 4. The novel antibacterial peptide is synthesized through the steps of splicing and optimizing the sequences of MurA nanobody, flexible Linker and Pediocin_PA-1 antibacterial peptide by using genetic engineering technology, and then inducing expression. The novel antibacterial peptide can specifically recognize MurA protein and Listeria monocytogenes whole bacteria, then under the action of the flexible Linker, the Pediocin_PA-1 antibacterial peptide is contacted with Listeria monocytogenes and plays a role of resisting Listeria monocytogenes, can target Listeria monocytogenes, has strong specificity, high inhibition effect, is non-toxic and harmless, and effectively improves the inhibition effect of the antibacterial peptide on Listeria monocytogenes.

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