A cell sorting device based on ultrasonic vibration microcavity

Abstract

The application relates to the technical field of biomedical engineering, and discloses a cell sorting device based on ultrasonic vibration microcavity, which comprises a sorting device body, a sorting machine, a circulating machine, a sheath liquid pipe and a pinch valve, the sorting machine is fixedly connected to the outer wall of the sorting device body, the circulating machine is fixedly connected to the outer wall of the sorting device body, the sheath liquid pipe is fixedly connected to the inside of the circulating machine, and the pinch valve is fixedly connected to the outer wall of the sorting device body; after the sheath liquid pipe is placed in the pinch valve, the limiting barrel is rotated to limit the sheath liquid pipe in the pinch valve, so that the sheath liquid pipe is fixed in the pinch valve without needing to be extruded, the problem that the sheath liquid pipe is extruded and deformed due to extrusion is avoided, the influence of the sheath liquid pipe on the sorting work due to the need of the pinch valve for fixation is reduced, the stability of the sorting work is improved, and the sheath liquid pipe is in an immovable state in the inner cavity of the pinch valve by rotating the limiting barrel.

Description

Technical Field

[0001] This invention relates to the field of biomedical engineering technology, specifically to a cell sorting device based on an ultrasonic vibration microcavity. Background Technology

[0002] A cell sorting device is a tool used to sort cells and manipulate cultured single cells. The cell analyzer uses a laser as a light source to illuminate the sample stream. Cells stained with fluorescence will produce scattered light and excitation fluorescence under the illumination of the laser beam. These signals are received by the corresponding photodetectors, converted into electrical signals, and then converted into digital signals that can be recognized by a computer through an analog-to-digital converter. When cells form droplets, they are charged according to the characteristic parameters set in the experimental design, and carry positive or negative charges. When the charged droplets fall into the high-voltage electrostatic field of the electrode deflection electrode, they will deflect to the right or left depending on the positive or negative of the charge they carry, and finally fall into the designated collector to complete the sorting.

[0003] When cells are sorted by giving them positive and negative charges, different types of cells need to be shifted to different positions. At this time, different cells are mixed inside the sheath fluid. If cells of different masses cannot be separated in time during sorting due to adhesion, different types of cells will be sorted to the same side at the same time, or they will be unable to move because they are attracted to each other by the charge. In the end, they will flow back with the unlabeled sheath fluid, which will cause misidentification of cell sorting and reduced sorting efficiency.

[0004] During the process of placing the sheath fluid tube into the pinch valve, the sheath fluid tube is squeezed into the pinch valve through the opening of the pinch valve. After being squeezed by the inner cavity of the pinch valve, the sheath fluid tube deforms and becomes an irregular shape. When the sheath fluid flows through the pinch valve, the flow rate of the sheath fluid will change due to the deformation of the sheath fluid tube, which will cause the equipment to be unable to sort different cells in time.

[0005] To address this, a cell sorting device based on an ultrasonic vibration microcavity is proposed. Summary of the Invention

[0006] The purpose of this invention is to provide a cell sorting device based on an ultrasonic vibration microcavity to solve the problems mentioned in the background art.

[0007] To achieve the above objectives, the present invention provides the following technical solution: a cell sorting device based on an ultrasonic vibration microcavity, comprising a sorting device body, a sorting machine, a circulating machine, a sheath fluid tube, and a clamping valve. The sorting machine is fixedly connected to the outer wall of the sorting device body, the circulating machine is fixedly connected to the outer wall of the sorting device body, the sheath fluid tube is fixedly connected to the interior of the circulating machine, and the clamping valve is fixedly connected to the outer wall of the sorting device body. A pre-separation component for pre-arranging cells in the sheath fluid of the sheath fluid tube is fixedly connected to the outer wall of the sorting device body and below the sorting machine. A limiting and fixing component to prevent the sheath fluid tube from falling off is provided inside the clamping valve, and a flow rate stabilizing component to stabilize the flow rate of the sheath fluid inside the sheath fluid tube is provided inside the clamping valve. The pre-separation assembly includes a first support symmetrically rotatably connected to the outer wall of the sorting device body. An ultrasonic generator is fixedly connected to the end of the first support away from the sorting device body. A second support is symmetrically rotatably connected to the outer wall of the sorting device body and below the first support. A vortex plate is fixedly connected to the outer wall of the second support. A base is symmetrically fixedly connected to the outer wall of the sorting device body and below the first support. A first sliding plate is slidably connected inside the base. A first round rod is symmetrically fixedly connected to the outer wall of the first sliding plate away from the sheath fluid tube. The first round rod is slidably connected to the side wall of the base. A second round rod is rotatably connected to the top of the base. A first spring is symmetrically fixedly connected between the first round rod and the second round rod. The side wall of the vortex plate and the side wall of the first round rod are in contact with each other. An arc-shaped push plate is fixedly connected to the side of the first sliding plate near the sheath fluid tube. A second spring is fixedly connected to the upper surface of the first sliding plate near the sheath fluid tube. The top of the second spring is fixedly connected to the outer wall of the ultrasonic generator.

[0008] Preferably, the limiting and fixing assembly includes a first slide rod slidably connected to the top of the clamp valve, a first circular plate magnetically adsorbed on the top of the first slide rod, a third slide rod slidably connected inside the first circular plate, a rack fixedly connected to the lower surface of the first slide rod, a second slide rod fixedly connected to the lower surface of the first slide rod, the second slide rod being slidably connected to the inside of the clamp valve, a limiting barrel rotatably connected inside the clamp valve, and an annular toothed plate fixedly connected to the outer wall of the limiting barrel, the annular toothed plate meshing with the rack.

[0009] Preferably, the flow rate stabilizing component includes a second circular plate fixedly connected to the top of the pinch valve; a turntable rotatably connected to the outer wall of the pinch valve; a third spring fixedly connected between the turntable and the second circular plate; a fourth slide rod slidably connected inside the turntable; a pressure plate fixedly connected to the end of the fourth slide rod away from the turntable; a fourth spring fixedly connected between the turntable and the pressure plate; the fourth spring sleeved on the outer wall of the fourth slide rod; a circular toothed plate fixedly connected to the lower surface of the turntable; open toothed plates symmetrically rotatably connected to the inside of the pinch valve with a limiting barrel; the circular toothed plates and open toothed plates meshing with each other; a limiting frame rotatably connected to the outer wall of the open toothed plates in a ring array; support rods rotatably connected to the inside of the pinch valve in a ring array; all support rods slidably connected to the inside of the limiting frame; a clamping plate rotatably connected to the side of the support rod away from the rotatably connected to the pinch valve; a locking plate slidably connected inside the turntable; a fifth spring fixedly connected between the locking plate and the turntable; and the end of the locking plate inside the turntable abutting against the lower surface of the circular toothed plate.

[0010] Preferably, the ultrasonic generator is symmetrical about both sides of the sheath fluid tube with the sheath fluid tube as the center, the radius of the arc-shaped push plate is equal to the radius of the sheath fluid tube, and the distance between the axis of the vortex plate and the axis of the first round rod changes with the rotation of the vortex plate.

[0011] Preferably, the base and the first slide plate have interpenetrating transverse grooves inside, the second support is movably connected inside the transverse grooves of the base and the first slide plate, and the sound wave frequencies of the two ultrasonic generators change periodically.

[0012] Preferably, the third and fourth slide rods are provided with a magnetic coating on the side that is close to each other, and the magnetic poles of the magnetic coatings of the third and fourth slide rods are opposite.

[0013] Preferably, the width of the opening of the limiting barrel is equal to the diameter of the sheath fluid tube, and a slot that matches the shape and size of the third slide rod is provided on the side of the turntable near the third slide rod.

[0014] Preferably, a torsion spring is sleeved at the rotatable connection between the clamping plate and the support rod, and the radius of the clamping plate is equal to the radius of the sheath fluid tube.

[0015] Preferably, the card plate is engaged with the circular toothed plate.

[0016] Compared with the prior art, the beneficial effects of the present invention are: 1. After the sheath fluid tube is placed inside the pinch valve, the limiting barrel is rotated to confine the sheath fluid tube inside the pinch valve. This eliminates the need for the sheath fluid tube to be fixed inside the pinch valve by squeezing, avoiding the problem of deformation caused by squeezing the sheath fluid tube. It also reduces the impact of the sheath fluid tube needing to be fixed by the pinch valve on the sorting process, improving the stability of the sorting process. By rotating the limiting barrel, the sheath fluid tube is kept in a fixed state inside the pinch valve, preventing the operator from accidentally pulling the sheath fluid tube out of the pinch valve during use, thus avoiding the problem of ineffective sorting due to the sheath fluid tube falling off. 2. The sheath fluid tube is synchronously covered and clamped by clamps arranged in a ring array, so that the cross-section of the sheath fluid tube after being inserted into the clamp valve can be clamped into a circular shape. This avoids the cross-section of the sheath fluid tube from deforming into an elliptical shape due to bending when it is close to the clamp valve, and avoids the change in the shape of the inner cavity of the sheath fluid tube at the bend, which would affect the flow of the sheath fluid inside the sheath fluid tube. This improves the stability of the sheath fluid as it flows through the clamp valve inside the sheath fluid tube. At the same time, the clamping and fixing of the sheath fluid tube by multiple clamps reduces the impact of sheath fluid tube shaking on the sorting results during the sorting process, and improves the accuracy of the sorting results. 3. By alternating the intensity of sound waves from symmetrical ultrasonic generators, different cells in the sheath fluid inside the sheath tube are displaced by different distances under the action of the ultrasonic waves. This allows different types of cells to be pre-separated in the sheath fluid before sorting, preventing the problem of different types of cells mixing and sticking together and blocking each other when entering the sorting machine, thus avoiding the problem of them being unable to be sorted. Through the relative setting and repeated upward rotation of the ultrasonic generators, the cells in the sheath fluid are pushed upward in a spiral shape into the sorting machine, ensuring that all cells entering the sorting machine are in a separated state, thereby ensuring efficient sorting. 4. By tapping and vibrating the sheath fluid tube with symmetrical arc-shaped push plates, air bubbles attached to the inside of the sheath fluid tube can be vibrated, causing the air bubbles to detach from the inner wall of the sheath fluid tube and float upwards. This avoids the air bubbles interfering with the flow of the sheath fluid inside the sheath fluid tube. By eliminating air bubbles inside the sheath fluid tube, interference with the cell labeling signal can be avoided. Removing air bubbles ensures the stability of the electrical signal, thereby ensuring the reliability of the sorting results. The tapping and vibration of the sheath fluid tube can also prevent the accumulation of air bubbles at the bends of the sheath fluid tube, and prevent excessively large air bubbles from obstructing the flow of the sheath fluid in the sheath fluid tube. Attached Figure Description

[0017] Figure 1 This is a three-dimensional schematic diagram of the overall structure of the present invention; Figure 2 This is a partial schematic diagram of the pre-separation component structure of the present invention; Figure 3 For the present invention Figure 2Enlarged schematic diagram of the structure at point A in the middle; Figure 4 This is an exploded view of the pre-separation component structure of the present invention; Figure 5 This is a cross-sectional view of the internal structure of the pinch valve of the present invention; Figure 6 This is a cross-sectional schematic diagram of the flow rate stabilization component structure of the present invention; Figure 7 This is an exploded view of the internal structure of the pinch valve of the present invention; Figure 8 This is a partial cross-sectional view of the flow rate stabilization component structure of the present invention.

[0018] In the picture: 11. Main body of the sorting device; 12. Sorter; 13. Circulator; 14. Sheath liquid tube; 15. Pinch valve; 2. Pre-separation assembly; 21. First support; 22. Ultrasonic generator; 23. Second support; 24. Vortex plate; 25. Base; 26. First sliding plate; 27. First round rod; 28. Second round rod; 29. ​​First spring; 210. Arc-shaped push plate; 211. Second spring; 3. Limiting and fixing assembly; 31. First slide bar; 32. Rack; 33. Second slide bar; 34. Limiting barrel; 35. Annular toothed plate; 36. First circular plate; 37. Third slide bar; 4. Flow rate stabilizing component; 41. Second circular plate; 42. Turntable; 43. Third spring; 44. Fourth slide bar; 45. Pressure plate; 46. Fourth spring; 47. Circular toothed plate; 48. Open toothed plate; 49. Limiting frame; 410. Support rod; 411. Clamping plate; 412. Card plate; 413. Fifth spring. Detailed Implementation

[0019] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present invention.

[0020] Embodiments of the present invention Please see Figures 1 to 8A cell sorting device based on an ultrasonic vibration microcavity includes a sorting device body 11, a sorting machine 12, a circulating machine 13, a sheath fluid tube 14, and a clamp valve 15. The sorting machine 12 is fixedly connected to the outer wall of the sorting device body 11, the circulating machine 13 is fixedly connected to the outer wall of the sorting device body 11, the sheath fluid tube 14 is fixedly connected to the inside of the circulating machine 13, and the clamp valve 15 is fixedly connected to the outer wall of the sorting device body 11. A pre-separation component 2 for pre-arranging cells in the sheath fluid of the sheath fluid tube 14 is fixedly connected to the outer wall of the sorting device body 11 and below the sorting machine 12. A limiting and fixing component 3 for preventing the sheath fluid tube 14 from falling off is provided inside the clamp valve 15, and a flow rate stabilizing component 4 for stabilizing the flow rate of the sheath fluid inside the sheath fluid tube 14 is provided inside the clamp valve 15. The pre-separation assembly 2 includes a first support 21 symmetrically rotatably connected to the outer wall of the sorting device body 11. An ultrasonic generator 22 is fixedly connected to the end of the first support 21 away from the sorting device body 11. A second support 23 is symmetrically rotatably connected to the outer wall of the sorting device body 11 and below the first support 21. A vortex plate 24 is fixedly connected to the outer wall of the second support 23. A base 25 is symmetrically fixedly connected to the outer wall of the sorting device body 11 and below the first support 21. A first sliding plate 26 is slidably connected inside the base 25. The side of the outer wall of the first sliding plate 26 away from the sheath fluid tube 14 is... A first round rod 27 is symmetrically fixedly connected, and the first round rod 27 is slidably connected to the side wall of the base 25. A second round rod 28 is rotatably connected to the top of the base 25. A first spring 29 is symmetrically fixedly connected between the first round rod 27 and the second round rod 28. The side wall of the vortex plate 24 is in contact with the side wall of the first round rod 27. An arc-shaped push plate 210 is fixedly connected to the side of the first slide plate 26 near the sheath fluid tube 14. A second spring 211 is fixedly connected to the upper surface of the first slide plate 26 near the sheath fluid tube 14. The top of the second spring 211 is fixedly connected to the outer wall of the ultrasonic generator 22.

[0021] The limiting and fixing assembly 3 includes a first slide rod 31 slidably connected to the top of the clamp valve 15. The top of the first slide rod 31 is magnetically attracted to a first circular plate 36. The inside of the first circular plate 36 is slidably connected to a third slide rod 37. The lower surface of the first slide rod 31 is fixedly connected to a rack 32. The lower surface of the first slide rod 31 is fixedly connected to a second slide rod 33. The second slide rod 33 is slidably connected to the inside of the clamp valve 15. The inside of the clamp valve 15 is rotatably connected to a limiting barrel 34. The outer wall of the limiting barrel 34 is fixedly connected to an annular toothed plate 35. The annular toothed plate 35 and the rack 32 mesh with each other.

[0022] The flow rate stabilizing component 4 includes a second circular plate 41 fixedly connected to the top of the pinch valve 15. A turntable 42 is rotatably connected to the outer wall of the pinch valve 15. A third spring 43 is fixedly connected between the turntable 42 and the second circular plate 41. A fourth slide rod 44 is slidably connected inside the turntable 42. A pressure plate 45 is fixedly connected to the end of the fourth slide rod 44 away from the turntable 42. A fourth spring 46 is fixedly connected between the turntable 42 and the pressure plate 45. The fourth spring 46 is sleeved on the outer wall of the fourth slide rod 44. A circular toothed plate 47 is fixedly connected to the lower surface of the turntable 42. Open teeth are symmetrically rotatably connected inside the pinch valve 15 with respect to the limiting barrel 34. Plate 48, circular toothed plate 47 and open toothed plate 48 mesh with each other. The outer wall of open toothed plate 48 is rotatably connected to limit frame 49 in a ring array. The inside of clamp valve 15 is rotatably connected to support rods 410 in a ring array. All support rods 410 are slidably connected to the inside of limit frame 49. The side of support rod 410 away from the rotatably connected to clamp valve 15 is rotatably connected to clamp plate 411. The inside of turntable 42 is slidably connected to clamp plate 412. A fifth spring 413 is fixedly connected between clamp plate 412 and turntable 42. One end of clamp plate 412 inside turntable 42 abuts against the lower surface of circular toothed plate 47.

[0023] The ultrasonic generator 22 is symmetrical about the sheath fluid tube 14 on both sides of the sheath fluid tube 14. The radius of the arc-shaped push plate 210 is equal to the radius of the sheath fluid tube 14. The distance between the axis of the vortex plate 24 and the axis of the first round rod 27 changes with the rotation of the vortex plate 24.

[0024] The base 25 and the first slide plate 26 have intersecting transverse grooves inside. The second support 23 is movably connected inside the transverse grooves of the base 25 and the first slide plate 26. The sound wave frequencies of the two ultrasonic generators 22 change periodically.

[0025] The third slide bar 37 and the fourth slide bar 44 are both provided with a magnetic coating on the side that is close to each other, and the magnetic poles of the magnetic coatings of the third slide bar 37 and the fourth slide bar 44 are opposite.

[0026] The width of the opening of the limiting barrel 34 is equal to the diameter of the sheath fluid tube 14, and a slot that matches the shape and size of the third slide rod 37 is provided on the side of the turntable 42 near the third slide rod 37.

[0027] A torsion spring is fitted at the rotatable connection between the clamping plate 411 and the support rod 410. The radius of the clamping plate 411 is equal to the radius of the sheath fluid tube 14.

[0028] The clamping plate 412 is engaged with the circular toothed plate 47.

[0029] The working principle of the above implementation is as follows: The initialization steps are as follows: The opening of the limiting barrel 34 is connected to the opening of the clamp valve 15, and the third spring 43 is in an untwisted state.

[0030] The operation steps are as follows: The sheath fluid tube 14 is confined inside the pinch valve 15 by the limiting and fixing component 3: like Figures 5 to 8 As shown, during use, the operator inserts the sheath fluid tube 14 into the inside of the clamp valve 15 through the slot. Then, the operator presses down on the first circular plate 36. After the first circular plate 36 is pressed down, it drives the first slide rod 31 to slide into the inside of the clamp valve 15. During the downward movement of the first slide rod 31, it drives the rack 32 and the second slide rod 33 to move downward together. During the downward movement of the rack 32, it drives the limiting barrel 34, which meshes with it, to rotate. After the limiting barrel 34 rotates, its opening rotates to the top, so that the sheath fluid tube 14 penetrating inside the limiting barrel 34 cannot be removed from the inside of the clamp valve 15 due to the obstruction of the limiting barrel 34 and the inner cavity of the clamp valve 15. This means that when the limiting and fixing assembly 3 limits the sheath fluid tube 14 inside the clamp valve 15, it does not need to limit the sheath fluid tube 14 by squeezing, thus avoiding the deformation of the sheath fluid tube 14 due to squeezing, which would cause the flow rate of the sheath fluid inside the sheath fluid tube 14 to change due to the deformation of the sheath fluid tube 14.

[0031] The flow rate stabilization component 4 ensures a stable flow rate of the sheath fluid inside the sheath fluid tube 14. When the first circular plate 36 is pressed into the turntable 42, the lower surface of the first circular plate 36 will adhere to the upper surface of the clamp valve 15. The fourth slide rod 44 magnetically attracts the third slide rod 37 inside the first circular plate 36 through the magnetic coating, causing the third slide rod 37 to slide out from the inside of the first circular plate 36 and slide into the slot of the turntable 42. When the third slide rod 37 slides into the slot of the turntable 42, the turntable 42 and the first circular plate 36 are connected due to the common limiting of the third slide rod 37. Then, the operator rotates the turntable 42. During the rotation of the turntable 42, the third slide rod 37 will be activated. Spring 43 twists, generating a torque opposite to the rotation direction of turntable 42. As turntable 42 rotates, it drives the circular toothed plate 47 to rotate as well. During rotation, the circular toothed plate 47 presses against the side of clamping plate 412, causing it to slide outwards towards the clamping valve 15. When the circular toothed plate 47 stops rotating, it no longer presses against the side of clamping plate 412. The rebound torque of the third spring 43 causes the circular toothed plate 47 to tend to rotate in the opposite direction. When the circular toothed plate 47 rotates in the opposite direction, it is blocked by clamping plate 412 and cannot move, thus preventing the clamping valve from moving. Plate 412 and circular toothed plate 47 are in a snap-fit ​​state. During the rotation of circular toothed plate 47, it will drive the open toothed plate 48 inside the pinch valve 15, which meshes with circular toothed plate 47, to rotate together. After the open toothed plate 48 rotates, it will drive the limiting frame 49, which is rotatably connected to its outer wall, to rotate together with the open toothed plate 48. During the rotation of the limiting frame 49, it will drive the sliding support rod 410 inside it to rotate together. Since one end of the support rod 410 is rotatably connected to the inner cavity of the pinch valve 15, when the open toothed plate 48 drives the limiting frame 49 to rotate together, the support rod 410 sliding inside the limiting frame 49 will rotate. Rotating around the pivot connection between the strut 410 and the inner cavity of the clamp valve 15, the strut 410 rotates, causing the clamp plate 411 to move towards the sheath fluid tube 14. The clamp plates 411, arranged in a ring array, move towards the sheath fluid tube 14 simultaneously and squeeze it. After the outer wall of the sheath fluid tube 14 is squeezed evenly by the clamp plate 411 in a ring, the cross-sectional shape of the sheath fluid tube 14 is adjusted from ellipse to circle. This avoids the sheath fluid tube 14 from being bent into an ellipse due to bending and folding at one end inside the clamp valve 15, thus improving the stability of the sheath fluid flow inside the sheath fluid tube 14.

[0032] The cells in the sheath fluid are pre-separated by alternating changes in the intensity of sound waves generated by the ultrasonic generator 22. like Figures 1 to 4As shown, when the sheath fluid flows from the circulation machine 13 into the sorting machine 12 through the sheath fluid tube 14, it first undergoes ultrasonic pre-separation through ultrasonic generators 22 symmetrically arranged below the sorting machine 12. During use, the intensity of the ultrasonic waves emitted by the symmetrical ultrasonic generators 22 alternates between high and low. Because the cells that need to be sorted inside the sheath fluid have different volumes, the displacement of cells of different masses after being impacted by ultrasonic waves is proportional to their own volume. Under the alternating impact of the symmetrical ultrasonic generators 22, the larger cells flow in a spiral shape inside the sheath fluid tube 14 and are close to the inner part of the sheath fluid tube 14. Under the alternating strong and weak impacts of the symmetrical ultrasonic generator 22, smaller cells will also flow in a spiral shape inside the sheath fluid tube 14. However, because of their smaller mass, the amplitude of their displacement after being impacted by the ultrasonic waves is smaller than that of larger cells. Therefore, the distance between them and the inner wall of the sheath fluid tube 14 is greater than the distance between larger cells and the inner wall of the sheath fluid tube 14. By alternating the ultrasonic intensity of the symmetrical ultrasonic generator 22, cells of different masses in the sheath fluid tube 14 are pre-separated to avoid the large and small particles from entering the sorting machine 12 at the same time, which would affect the accuracy of sorting due to mutual mixing. Air bubbles are removed from the inside of the sheath fluid tube 14 by the pre-separation component 2: During the sorting process, the second support 23 rotates, causing the vortex plate 24 to rotate as well. Due to the shape of the vortex plate 24, its rotation first compresses the first round rod 27. After being compressed, the first round rod 27 causes the first slide plate 26 to slide inside the base 25, stretching the first spring 29. When the outwardly expanding side of the vortex plate 24 is no longer in contact with the first round rod 27, the first spring 29 is no longer stretched and quickly contracts elastically. The contraction of the first spring 29 causes the first slide plate 26 to slide inside the base 25 towards the side closer to the sheath fluid tube 14. The sliding of the first slide plate 26 causes the arc-shaped push plate 210 to move together and impact the sheath fluid tube 14, causing it to vibrate. The symmetrical arc-shaped push plate 210 impacts the sheath fluid tube 14, causing the air bubbles attached to the inner wall of the sheath fluid tube 14 to detach from the sheath due to the vibration of the sheath fluid tube 14. The inner wall of the liquid tube 14 floats upward. When the outer expansion of the outer wall of the vortex plate 24 comes into contact with the first round rod 27 again, the first round rod 27 is squeezed and drives the first slide plate 26 and the arc-shaped push plate 210 to slide away from the side of the sheath fluid tube 14 inside the base 25. This causes the arc-shaped push plate 210 to repeatedly impact the sheath fluid tube 14 during the cell sorting process. During the sliding of the first round rod 27 inside the base 25, it will drive the ultrasonic generator 22 to rotate on the first support 21 through the second spring 211. This causes the symmetrical ultrasonic generators 22 to rotate upward synchronously from opposite directions. During the synchronous upward rotation of the symmetrical ultrasonic generators 22, the shock wave generated by the ultrasonic generators 22 will push the cells in the sheath fluid inside the sheath fluid tube 14 upward, so that the cells of different sizes in the sheath fluid remain separated before entering the sorting machine 12.

[0033] It should be noted that, in this document, relational terms such as "first" and "second" are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0034] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A cell sorting device based on an ultrasonic vibration microcavity, comprising a sorting device body (11), a sorter (12), a circulator (13), a sheath fluid tube (14), and a clamp valve (15), characterized in that: The sorting machine (12) is fixedly connected to the outer wall of the main body (11) of the sorting device. The circulating machine (13) is fixedly connected to the outer wall of the main body (11) of the sorting device. The sheath fluid tube (14) is fixedly connected to the inside of the circulating machine (13). The clamp valve (15) is fixedly connected to the outer wall of the main body (11) of the sorting device. A pre-separation component (2) for pre-arranging cells in the sheath fluid of the sheath fluid tube (14) is fixedly connected to the outer wall of the main body (11) of the sorting device and below the sorting machine (12). A limiting and fixing component (3) for preventing the sheath fluid tube (14) from falling off is provided inside the clamp valve (15). A flow rate stabilizing component (4) for stabilizing the flow rate of the sheath fluid inside the sheath fluid tube (14) is provided inside the clamp valve (15). The pre-separation component (2) includes a first support (21) symmetrically rotatably connected to the outer wall of the sorting device body (11). An ultrasonic generator (22) is fixedly connected to the end of the first support (21) away from the sorting device body (11). A second support (23) is symmetrically rotatably connected to the outer wall of the sorting device body (11) and below the first support (21). A vortex plate (24) is fixedly connected to the outer wall of the second support (23). A base (25) is symmetrically fixedly connected to the outer wall of the sorting device body (11) and below the first support (21). A first sliding plate (26) is slidably connected inside the base (25). The side of the outer wall of the first sliding plate (26) away from the sheath fluid tube (14) is... A first round rod (27) is symmetrically fixedly connected to the side wall of the base (25). The top of the base (25) is rotatably connected to a second round rod (28). A first spring (29) is symmetrically fixedly connected between the first round rod (27) and the second round rod (28). The side wall of the vortex plate (24) is in contact with the side wall of the first round rod (27). An arc-shaped push plate (210) is fixedly connected to the side of the first slide plate (26) near the sheath fluid tube (14). A second spring (211) is fixedly connected to the side of the upper surface of the first slide plate (26) near the sheath fluid tube (14). The top of the second spring (211) is fixedly connected to the outer wall of the ultrasonic generator (22).

2. The cell sorting device based on ultrasonic vibration microcavity according to claim 1, characterized in that: The limiting and fixing assembly (3) includes a first slide rod (31) slidably connected to the top of the clamp valve (15). The top of the first slide rod (31) is magnetically attracted to a first circular plate (36). The interior of the first circular plate (36) is slidably connected to a third slide rod (37). The lower surface of the first slide rod (31) is fixedly connected to a rack (32). The lower surface of the first slide rod (31) is fixedly connected to a second slide rod (33). The second slide rod (33) is slidably connected to the interior of the clamp valve (15). The interior of the clamp valve (15) is rotatably connected to a limiting barrel (34). The outer wall of the limiting barrel (34) is fixedly connected to an annular toothed plate (35). The annular toothed plate (35) and the rack (32) mesh with each other.

3. The cell sorting device based on ultrasonic vibration microcavity according to claim 2, characterized in that: The flow rate stabilizing component (4) includes a second circular plate (41) fixedly connected to the top of the pinch valve (15). A turntable (42) is rotatably connected to the outer wall of the pinch valve (15). A third spring (43) is fixedly connected between the turntable (42) and the second circular plate (41). A fourth slide rod (44) is slidably connected inside the turntable (42). A pressure plate (45) is fixedly connected to the end of the fourth slide rod (44) away from the turntable (42). A fourth spring (46) is fixedly connected between the turntable (42) and the pressure plate (45). The fourth spring (46) is sleeved on the outer wall of the fourth slide rod (44). A circular toothed plate (47) is fixedly connected to the lower surface of the turntable (42). A limiting barrel (34) is symmetrically rotatably connected inside the pinch valve (15). An open toothed plate (48) is formed, and a circular toothed plate (47) meshes with the open toothed plate (48). The outer wall of the open toothed plate (48) is arranged in a ring array and rotatably connected to a limiting frame (49). The inside of the clamp valve (15) is arranged in a ring array and rotatably connected to a support rod (410). The support rod (410) is slidably connected to the inside of the limiting frame (49). The side of the support rod (410) away from the rotatably connected to the clamp valve (15) is rotatably connected to a clamping plate (411). The inside of the turntable (42) is slidably connected to a locking plate (412). A fifth spring (413) is fixedly connected between the locking plate (412) and the turntable (42). One end of the locking plate (412) located inside the turntable (42) abuts against the lower surface of the circular toothed plate (47).

4. The cell sorting device based on an ultrasonic vibration microcavity according to claim 1, characterized in that: The ultrasonic generator (22) is symmetrical about the sheath tube (14) on both sides of the sheath tube (14). The radius of the arc-shaped push plate (210) is equal to the radius of the sheath tube (14). The distance between the axis of the vortex plate (24) and the axis of the first round rod (27) changes with the rotation of the vortex plate (24).

5. The cell sorting device based on an ultrasonic vibration microcavity according to claim 1, characterized in that: The base (25) and the first slide plate (26) are provided with interpenetrating transverse sliding grooves. The second support (23) is movably connected to the transverse sliding grooves of the base (25) and the first slide plate (26). The sound wave frequencies of the two ultrasonic generators (22) change periodically.

6. The cell sorting device based on an ultrasonic vibration microcavity according to claim 3, characterized in that: The third slide bar (37) and the fourth slide bar (44) are provided with magnetic coatings on their adjacent sides, and the magnetic poles of the magnetic coatings of the third slide bar (37) and the fourth slide bar (44) are opposite.

7. The cell sorting device based on an ultrasonic vibration microcavity according to claim 3, characterized in that: The width of the opening of the limiting barrel (34) is equal to the diameter of the sheath fluid tube (14), and a slot that matches the shape and size of the third slide rod (37) is provided on the side of the turntable (42) near the third slide rod (37).

8. A cell sorting device based on an ultrasonic vibration microcavity according to claim 3, characterized in that: A torsion spring is fitted at the rotatable connection between the clamp (411) and the support rod (410), and the radius of the clamp (411) is equal to the radius of the sheath fluid tube (14).

9. A cell sorting device based on an ultrasonic vibration microcavity according to claim 3, characterized in that: The card plate (412) is engaged with the circular toothed plate (47).

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