A dual-path identification system for the codling moth, integrating RPA-LFS rapid screening and TaqMan qPCR real-time quantification, along with its locked nucleic acid probe primer set, kit, and method.

By integrating RPA-LFS and TaqMan qPCR into a dual-pathway identification system for codling moth, using specific primer and probe sets, the problem of rapid and convenient identification of codling moth and closely related insects is solved, achieving efficient and accurate detection of codling moth, suitable for field and on-site testing.

CN122104952APending Publication Date: 2026-05-29NINGBO UNIV +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NINGBO UNIV
Filing Date
2026-04-24
Publication Date
2026-05-29

AI Technical Summary

Technical Problem

Existing technologies make it difficult to quickly, easily, and accurately distinguish the codling moth from other closely related insects, especially in port quarantine, resulting in time-consuming and labor-intensive processes with a high rate of misidentification, which affects the efficiency of agricultural trade and quarantine.

Method used

A dual-pathway identification system for codling moths was adopted, integrating RPA-LFS rapid screening with TaqMan qPCR real-time quantification. Specific primer and probe sets were used to detect codling moths. By combining RPA-LFS and TaqMan qPCR technologies, rapid and convenient identification of codling moths was achieved.

Benefits of technology

It enables rapid and accurate identification of the codling moth, and can detect codling moth eggs, larvae, pupae and adults in the field and on-site. It has extremely high specificity and sensitivity, and is suitable for plant quarantine and control, reducing the false identification rate and detection cost.

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Abstract

The present application relates to the technical field of biological detection, and a double-path apple codling moth identification system integrating RPA-LFS rapid screening and TaqMan qPCR real-time quantification, a locked nucleic acid probe primer set, a kit and a method thereof. COI Through gene screening and primer screening, the present application designs recombinase-mediated isothermal amplification (RPA) and TaqMan qPCR primers and probes for the barcode of the cytochrome oxidase subunit I (COI) gene of apple codling moth, and establishes an RPA-LFS method in combination with immunolateral flow. The RPA-LFS method can complete amplification and visual judgment within 20 minutes at 39 DEG C, and is suitable for on-site rapid detection. The TaqMan qPCR method can realize high-sensitivity quantitative analysis under the condition of crude extract. Through double-path complementary design, the consistency of the results of on-site screening and laboratory confirmation detection is realized, and the method is simple to operate and suitable for pest quarantine at ports, epidemic monitoring in agricultural production areas and quarantine at storage and transportation links.
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