A method for detecting a pathogenic human parasite

Abstract

The present application relates to the field of parasitic detection method, more particularly, to a kind of etiology parasitic detection method of human body, comprising the following steps;Step 1, add clean water in the culture hole of cell culture plate, take a proper amount of feces and place in clean water and stir evenly;Step 2, the fecal liquid in the culture hole of cell culture plate is transferred to test tube, then the test tube is placed into centrifuge and centrifuged;Step 3, the supernatant in test tube is taken out and placed in another test tube;Step 4, repeat step 2 and step 3 until the fecal liquid is clear;Step 5, take the supernatant of step 4, collect pathogen;Step 6, the supernatant of step 5 is examined by inverted microscope, and the parasitic detection of human body is carried out.

Description

Technical Field

[0001] This invention relates to the field of parasite detection methods, and more specifically, to a method for detecting human parasites through etiology. Background Technology

[0002] In the etiological diagnosis of human parasitic diseases, centrifugation sedimentation is a commonly used fecal detection technique in laboratories, especially in epidemiological surveys. This method aims to enrich pathogens such as parasite eggs and cysts in samples through centrifugation to improve the detection rate. The standard operating procedure is as follows: Take a 1.5 ml (or 15 ml) centrifuge tube and add approximately 1 ml (or 10 ml) of water; use a bamboo skewer to pick up a sample of fecal matter about the size of a soybean (1-2 grams) and place it in the tube, mixing thoroughly; then centrifuge at 1000-2000 rpm for 1-2 minutes, discarding the supernatant; resuspend the precipitate in water, repeating the centrifugation and washing steps 3-4 times until the supernatant becomes clear; finally, discard the supernatant, add 100-300 μL (or 1 ml) of water to resuspend the precipitate, use a pipette to place a drop of the suspension onto a glass slide, cover with a coverslip, and observe under a regular optical microscope.

[0003] Although centrifugation precipitation can significantly improve the detection sensitivity of pathogens compared to direct smears, in practical applications, especially when further in-depth studies such as morphological verification, molecular biological identification, or culture are required, this technique has a serious limitation: it is difficult to effectively recover and collect specific pathogens found in microscopic examination.

[0004] Specifically, its drawbacks are reflected in the following aspects: The collection process after microscopic examination is cumbersome and has a low recovery rate: When suspected or typical pathogens (such as rare parasite eggs or protozoa) are found under a microscope, current methods are extremely inconvenient if the operator wishes to collect them for further research. Since the sample has already been prepared as a smear, the fecal fluid gradually dries under the coverslip, and the pathogens are firmly adsorbed onto the slide surface. At this point, the coverslip must be lifted, and the slide repeatedly rinsed with physiological saline or water. The rinsing solution must be collected in a centrifuge tube and centrifuged again to potentially recover some of the pathogens. This process is cumbersome, time-consuming, and due to the adsorption on the slide surface and losses during multiple transfers, the recovery rate of the target pathogen is extremely low, and it often fails, failing to meet the sample volume requirements for subsequent research.

[0005] Therefore, it is necessary to propose a pathogenic human parasite detection method to solve the above problems. Summary of the Invention

[0006] To overcome at least one of the defects (deficiencies) of the prior art described above, the present invention provides a method for detecting human parasites through etiology.

[0007] To solve the above-mentioned technical problems, the technical solution of the present invention is as follows: a method for detecting human parasites by pathogens, comprising the following steps; Step 1: Add water to the culture wells of the cell culture plate, take an appropriate amount of feces, put it in the water and stir well; Step 2: After transferring the fecal matter from the cell culture plate wells to test tubes, place the test tubes into a centrifuge for centrifugation. Step 3: Remove the supernatant from the test tube and place it in another test tube; Step 4: Repeat steps 2 and 3 until the fecal matter is clear; Step 5: Take the supernatant from Step 4 and collect the pathogens; Step 6: Examine the supernatant from Step 5 under an inverted microscope to detect human parasites. Furthermore, in step 2, the centrifuge speed is 1000-2000 rpm, and the time is 1-2 minutes.

[0008] Furthermore, in step 5, 100-200 microliters of the supernatant are taken.

[0009] Furthermore, step 6 includes the following steps: Step 61: Place the supernatant from step 5 into the culture wells of the cell culture plate; Step 62: Cover the culture wells with the cell plate caps and examine the supernatant from Step 5 under an inverted microscope to detect human parasites.

[0010] Furthermore, the cell culture plate is a 24-well cell culture plate, a 48-well cell culture plate, or a 96-well cell culture plate.

[0011] Compared with the prior art, the beneficial effects of the technical solution of the present invention are: The present invention discloses a method for detecting human parasites through etiology. Attached Figure Description

[0012] Figure 1 This is a flowchart of the method for detecting human parasites in pathogenicity according to the present invention. Detailed Implementation

[0013] The accompanying drawings are for illustrative purposes only and should not be construed as limiting the scope of this patent. To better illustrate this embodiment, some components in the drawings may be omitted, enlarged, or reduced, and do not represent the actual dimensions of the product. It is understandable to those skilled in the art that some well-known structures and their descriptions may be omitted in the drawings.

[0014] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation" and "connection" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication between two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances. The technical solution of this invention will be further described below with reference to the accompanying drawings and embodiments.

[0015] like Figure 1 As shown, a method for detecting human parasites by pathogens includes the following steps; Step 1: Add water to the culture wells of the cell culture plate, take an appropriate amount of feces, put it in the water and stir well; Step 2: After transferring the fecal matter from the cell culture plate wells to test tubes, place the test tubes into a centrifuge for centrifugation. Step 3: Remove the supernatant from the test tube and place it in another test tube; Step 4: Repeat steps 2 and 3 until the fecal matter is clear; Step 5: Take the supernatant from Step 4 and collect the pathogens; Step 6: Examine the supernatant from Step 5 under an inverted microscope to detect human parasites. In step 2 of this invention, the centrifuge speed is 1000-2000 rpm and the time is 1-2 minutes.

[0016] In step 5 of this invention, 100-200 microliters of the supernatant are taken.

[0017] Step 6 of this invention includes the following steps: Step 61: Place the supernatant from step 5 into the culture wells of the cell culture plate; Step 62: Cover the culture wells with the cell plate caps and examine the supernatant from Step 5 under an inverted microscope to detect human parasites.

[0018] In this invention, the cell culture plate is a 24-well cell culture plate, a 48-well cell culture plate, or a 96-well cell culture plate.

[0019] The positional relationships described in the figures are for illustrative purposes only and should not be construed as limiting this patent. Clearly, the above embodiments of the present invention are merely examples to clearly illustrate the invention and are not intended to limit the implementation of the invention. Those skilled in the art can make other variations or modifications based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the scope of protection of the claims of this invention.

Claims

1. A method for detecting human parasites through etiology, characterized in that: Includes the following steps; Step 1: Add water to the culture wells of the cell culture plate, take an appropriate amount of feces, put it in the water and stir well; Step 2: After transferring the fecal matter from the cell culture plate wells to test tubes, place the test tubes into a centrifuge for centrifugation. Step 3: Remove the supernatant from the test tube and place it in another test tube; Step 4: Repeat steps 2 and 3 until the fecal matter is clear; Step 5: Take the supernatant from Step 4 and collect the pathogens; Step 6: Examine the supernatant from Step 5 under an inverted microscope to detect human parasites.

2. The method for detecting human parasites according to claim 1, characterized in that: In step 2, the centrifuge speed is 1000-2000 rpm, and the time is 1-2 minutes.

3. The method for detecting human parasites according to claim 1, characterized in that: In step 5, take 100-200 microliters of the supernatant.

4. The method for detecting human parasites according to claim 1, characterized in that: Step 6 includes the following steps: Step 61: Place the supernatant from step 5 into the culture wells of the cell culture plate; Step 62: Cover the culture wells with the cell plate caps and examine the supernatant from Step 5 under an inverted microscope to detect human parasites.

5. The method for detecting human parasites according to claim 1, characterized in that: The cell culture plate is a 24-well cell culture plate, a 48-well cell culture plate, or a 96-well cell culture plate.

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