A rapid detection method for cleanliness of a slide for chromosome preparation for visual analysis

By using a detection solution composed of phenol red, anhydrous methanol, and glacial acetic acid, systematic sampling and testing of pathological slides was conducted. This solved the problem of diffusion instability caused by batch-to-batch variations in slide quality, enabling rapid and intuitive detection of slide cleanliness and improving the success rate of chromosome preparation and the reliability of experimental results.

CN122109098APending Publication Date: 2026-05-29SHAOXING WOMEN & CHILDRENS HOSPITAL
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHAOXING WOMEN & CHILDRENS HOSPITAL
Filing Date
2026-02-26
Publication Date
2026-05-29

AI Technical Summary

Technical Problem

The batch-to-batch variation in the quality of commercial slides leads to unstable diffusion quality of chromosome suspensions, affecting the uniformity and reproducibility of experiments and making it difficult to guarantee the accuracy of karyotype analysis.

Method used

A detection solution composed of phenol red, anhydrous methanol, and glacial acetic acid was used to systematically sample and test pathological slides. The cleanliness of the slides was judged by observing the diffusion rate and color characteristics of the detection solution on the slides. A multi-level judgment standard was established to ensure the accuracy of the test.

Benefits of technology

This technology enables rapid and intuitive detection of slide cleanliness, improves the success rate of chromosome preparation and the reliability of experimental results, and meets the quality control needs of clinical laboratories.

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Abstract

The application discloses a rapid detection method for the cleanliness of a slide for chromosome preparation for visual analysis, and relates to the technical field of chromosome detection, and comprises the following steps: step one, phenol red is added to anhydrous methanol and glacial acetic acid, the mixture is uniformly mixed, sealed and left to stand, after undissolved particles are precipitated, the supernatant clear orange liquid is taken as a detection reagent; step two, the system sampling method is used to screen and detect pathological slides in a whole box, and the same batch of pathological slides without defects and pollution are detected; step three, a plurality of pathological slides that pass the detection are selected and placed obliquely, and the detection reagent is added dropwise; and step four, the cleanliness of the pathological slides is judged. The visual rapid cleanliness detection method has the advantages of simple process, intuitive interpretation, effective satisfaction of the daily quality control demand of the slide for chromosome preparation in a laboratory and popularization value.
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