Method for detecting direct absorption of exogenous glycine content by microorganisms in soil

By using gas chromatography-mass spectrometry with 13C-15N double-labeled glycine and the internal standard alanine, the accuracy problem of detecting glycine content directly absorbed by soil microorganisms was solved, achieving high sensitivity and high accuracy detection results.

CN122109396BActive Publication Date: 2026-07-24SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
Filing Date
2026-04-28
Publication Date
2026-07-24

AI Technical Summary

Technical Problem

Existing technologies are difficult to accurately quantify the content of glycine directly absorbed and utilized by soil microorganisms, and are easily affected by matrix interference, leading to inaccurate test results.

Method used

Gas chromatography-mass spectrometry (GC-MS) was used to derivatize and separate glycine with 13C-15N double labeling and alanine as an internal standard. Characteristic ions were used to separate the m/z values, and the results were quantified using the internal standard method. Detection was performed using a gas GC-MS instrument.

Benefits of technology

This method enables highly sensitive, specific, and accurate detection of glycine content directly absorbed by soil microorganisms, reducing matrix interference and improving detection accuracy.

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Abstract

The application belongs to the field of analysis and detection, and particularly relates to a method for detecting the content of directly absorbed exogenous glycine of microorganisms in soil, which comprises the following steps: firstly, mixing a double-labeled glycine solution with soil, adding sodium laureth sulfate and chloroform for fumigation, then extracting, deriving by hydrocarbonation reaction of ethyl bromoformate, and completing the extraction of glycine in microorganisms after stable layering by using sodium bicarbonate solution; and after the extraction is cracked by a stable isotope ratio mass spectrometer, exogenous glycine and original glycine in microorganisms are distinguished by characteristic ions to be determined. The method can effectively detect the content of directly absorbed exogenous glycine of microorganisms, is simple in operation, strong in specificity, has a good linear relationship, high sensitivity and accuracy, and effectively solves the problem that the content of directly absorbed exogenous glycine of microorganisms cannot be directly quantified. Thus, the direct utilization pathway of soil microorganisms to exogenous amino acid substances is quantitatively judged.
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