Hydroponic biotoxicity cultivation device

Abstract

A hydroponic biological toxicity culture device includes a culture tank, a monitoring system, and a maintenance system. The culture tank includes a bottom and a body, with the body holding the water for culturing the organisms and the bottom connected to the bottom of the body. A sampling port is provided on the body. The monitoring system monitors the state parameters of the water within the tank. The maintenance system includes a contaminant input component, comprising a syringe pump, an aeration structure, a gaseous contaminant supply source, a liquid contaminant supply source, and a solid contaminant storage container. The gaseous contaminant supply source is connected to the interior of the tank via the aeration structure; the liquid contaminant supply source is connected to the interior of the tank via the syringe pump. An oxygen supply gas delivery pipe is connected to the culture tank, and the solid contaminant storage container is connected to the oxygen supply gas delivery pipe. The solid contaminants are introduced into the water by the airflow in the oxygen supply gas delivery pipe. This invention is applicable to toxicity experiments involving gaseous, liquid, and solid contaminants and can monitor water parameters in real time, belonging to the field of biological toxicity culture experimental technology.

Description

Technical Field

[0001] This invention relates to the field of biotoxicity culture experimental technology, specifically to a hydroponic biotoxicity culture device. Background Technology

[0002] Toxicity testing is a crucial component of biological research, investigating the effects of chemical substances on organisms. Its purpose is to determine the degree of toxicity and dose-response relationship of a chemical substance, its relative toxicity compared to other substances, specific toxic effects, and the mechanisms of toxicity, providing a theoretical basis for further toxicity testing. In industries such as aquaculture, fisheries, marine science, and environmental science, conducting toxicity tests is a vital means of evaluating the environmental impact of man-made engineering and production emissions.

[0003] Currently, when conducting aquatic biological toxicity experiments, the test organism is typically identified first. It is then placed in culture dishes with different concentrations of contaminants. With the support of temperature control, water filtration, and aeration equipment, the basic properties of the water, except for the contaminants, are ensured not to affect the health of the test organism. Finally, data such as the survival and health status of the test organism are collected to determine the relevant toxicity parameters.

[0004] Toxicity experiments are categorized into acute, subchronic, chronic, and cumulative types, but all require apparatus for culturing and raising the test organisms. Generally, aquaculture apparatus is designed for solid and liquid contaminants, allowing for easy control of contaminant concentrations in the culture liquid, with minimal changes in concentration during the experiment. However, for gaseous contaminants, existing experimental equipment and frameworks struggle to ensure stable contaminant concentrations and monitor their changes. Furthermore, the study "Animal Cell Mortality Rate in Bubble Reactors" (Tan Wensong, Dai Ganze, Chen Zhihong, et al. DOI:10.13345 / j.cjb.1996.04.018.) states that "the cell death rate in a bioreactor is directly proportional to the gas bubbling rate and inversely proportional to the reactor volume." Moreover, bubble size affects hydrodynamic conditions, further influencing the contaminant diffusion rate, and existing experimental apparatuses do not consider the impact of gas bubble flow on the water body. On the other hand, existing experimental apparatuses do not consider the influence of parameters such as temperature and salinity on contaminant concentrations, nor do they consider the impact of water cleanliness on contaminants. Summary of the Invention

[0005] In view of the technical problems existing in the prior art, the purpose of this invention is to provide a hydroponic biological toxicity cultivation device that is suitable for toxicity experiments of pollutants in three forms: gas, liquid and solid, and can monitor water parameters in real time.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: a hydroponic biological toxicity culture device, comprising a culture tank, a monitoring system, and a maintenance system; the culture tank includes a bottom and a body, the body being used to hold the water for culturing the organisms, the bottom being connected to the bottom of the body, and a sampling port being provided on the body; the monitoring system is used to monitor the state parameters of the water in the tank; the maintenance system includes a contaminant input component, which includes an injection pump, an aeration structure, a gaseous contaminant supply source, a liquid contaminant supply source, and a solid contaminant storage container; the gaseous contaminant supply source is connected to the interior of the tank via the aeration structure, which has multiple ventilation channels, through which gaseous contaminants form bubbles and enter the water; the liquid contaminant supply source is connected to the interior of the tank via the injection pump; an oxygen supply gas delivery pipe is connected to the culture tank, and the solid contaminant storage container is connected to the oxygen supply gas delivery pipe, containing powdered solid contaminants, which are carried into the water by the airflow in the oxygen supply gas delivery pipe.

[0007] As a preferred option, the bottom of the barrel has an installation hole that connects to the inside of the barrel body. The gas distribution structure is installed in the installation hole by means of threaded insertion, bayonet connection or bolt fixing. The gas distribution structure is a porous disc, a porous tube or an integrated tube bundle structure.

[0008] As a preferred option, the air distribution structure is available in various specifications, with different specifications having different numbers of air channels and / or different inner diameters. Different specifications of air distribution structures can be replaced as needed to control the number and / or size of air bubbles.

[0009] As a preferred embodiment, the gaseous pollutant supply source is connected to the gas distribution structure via a gaseous pollutant delivery pipeline, and a first pressure reducing valve is provided on the gaseous pollutant delivery pipeline; a second pressure reducing valve is provided on the oxygen supply gas delivery pipeline, and the second pressure reducing valve is located upstream of the solid pollutant storage device.

[0010] As a preferred embodiment, the output end of the injection pump is connected to the inside of the barrel via a first pipe; a one-way valve is provided on the first pipe, the oxygen supply gas delivery pipe, and the gaseous pollutant delivery pipe.

[0011] As a preferred embodiment, the monitoring system includes multiple monitoring elements, including one or more of a temperature sensor, a pH sensor, a dissolved oxygen concentration sensor, and a salinity sensor, which are installed on the inner wall of the tank.

[0012] As a preferred option, the maintenance system also includes a water bath thermostat and a self-cleaning component. The water bath thermostat controls the temperature of the water inside the tank by means of a water bath, and the self-cleaning component is a multi-stage filtration and adsorption device located outside the culture tank and connected to the tank body through a circulation pipe, used to filter and clean the water.

[0013] As a preferred option, the gaseous pollutant supply source is a high-pressure gas storage tank containing gaseous pollutants and carrier gas, and the liquid pollutant supply source is a liquid storage tank containing liquid pollutants.

[0014] As a preferred option, the carrier gas is air or nitrogen. The amount of gaseous pollutants input is controlled by controlling the concentration of gaseous pollutants in the carrier gas. The solid pollutant storage device is connected to the oxygen gas delivery pipeline via a metering feeder that can quantitatively deliver powder.

[0015] As a preferred option, it also includes a control system, which includes a touch display and a central control chip. The central control chip is connected to the monitoring system, the maintenance system and the touch display via electrical signals.

[0016] In summary, the present invention has the following advantages:

[0017] (1) This invention can be applied to pollutants in various forms, including solid, liquid, and gas, and is not limited to solids and liquids;

[0018] (2) The present invention deploys monitoring sensors in multiple locations, which increases the sensitivity of the system and maintains the concentration of the aquaculture water more quickly;

[0019] (3) This invention can be operated unattended. After the parameters are set, the system can automatically control the concentration level of pollutants to ensure the continuous stability of the breeding environment.

[0020] (4) The present invention uses a control system to complete functions such as data display, input, search and control through a touch screen, without using mechanical buttons, which is convenient to use.

[0021] (5) The present invention is designed with a gas distribution structure to control gas leakage, control bubble size, monitor the disturbance of water body by bubbles of different sizes, and analyze its impact on the test organisms according to the simulation requirements.

[0022] (6) The present invention can be equipped with a variety of sensors as needed, which can accurately monitor the water conditions, control experimental variables according to the monitoring situation, and accurately count the changes in the water.

[0023] (7) The present invention is equipped with multiple in-situ sampling ports, which facilitates in-situ monitoring of other parameters of aquaculture water.

[0024] (8) The water filtration system of the present invention is highly adaptable and can be set up as needed. The simplest is activated carbon filtration, and further systems such as reverse osmosis membranes can be used. Attached Figure Description

[0025] Figure 1 This is a schematic diagram of the three-dimensional structure of the culture tank.

[0026] Figure 2This is a schematic diagram of a hydroponic biological toxicity culture device.

[0027] Figure 3 This is a perspective structural diagram of the barrel.

[0028] Figure 4 This is a schematic diagram of the three-dimensional structure of the bottom of the bucket.

[0029] Figure 5 This is a schematic diagram of a porous disc-shaped air distribution structure.

[0030] In this design, 1 is the bottom of the container, 2 is the body of the container, 3 is the top cover, 4 is the solid contaminant storage container, 5 is the liquid contaminant supply source, 6 is the gaseous contaminant supply source, 7 is the injection pump, 8 is the first pressure reducing valve, 9 is the second pressure reducing valve, 10 is the check valve, 11 is the metering feeder, 12 is the monitoring element, 13 is the sampling port, 14 is the gas distribution structure, 15 is the touch display, 16 is the multi-stage filtration and adsorption device, and 17 is the oxygen cylinder. 101 is the first pipeline interface, 102 is the second pipeline interface, 103 is the third pipeline interface, and 104 is the input port. 1401 is the ventilation channel. Detailed Implementation

[0031] The present invention will now be described in further detail with reference to the accompanying drawings and specific embodiments.

[0032] Example 1

[0033] like Figures 1-4As shown, a hydroponic biological toxicity culture device includes a culture tank, a monitoring system, and a maintenance system. The culture tank includes a bottom 1 and a body 2. The body is used to hold the water in which the cultured organisms are cultured, and the bottom is connected to the bottom of the body. A sampling port 13 is provided on the body. The monitoring system is used to monitor the state parameters of the water in the body. The maintenance system includes a contaminant input component, which includes an injection pump 7, an aeration structure 14, a gaseous contaminant supply source 6, a liquid contaminant supply source 5, and a solid contaminant storage container 4. The gaseous contaminant supply source is connected to the inside of the body through the aeration structure, which has multiple ventilation channels 1401. The gaseous contaminants enter the water as bubbles through the ventilation channels. The liquid contaminant supply source is connected to the inside of the body through the injection pump. An oxygen supply gas delivery pipe is connected to the culture tank, and the solid contaminant storage container is connected to the oxygen supply gas delivery pipe. The solid contaminant storage container contains powdered solid contaminants, which are carried into the water by the airflow in the oxygen supply gas delivery pipe. In use, the input amount of gaseous contaminants is controlled by the concentration of gaseous contaminants in the gaseous contaminant supply source, and the input amount of liquid contaminants is controlled by an injection pump. The solid contaminant storage tank is located above the oxygen gas supply pipeline and is connected to the oxygen gas supply pipeline via a metering feeder 11. The metering feeder can be, but is not limited to, a commercially available gear-type metering feeder, which uses the sealed gap formed by gear meshing to quantitatively deliver contaminants, thereby controlling the input amount of solid contaminants. One end of the oxygen gas supply pipeline connects to the inside of the tank, and the other end is connected to an oxygen cylinder 17.

[0034] The culture vessel is made entirely of transparent material and is a long cylindrical barrel, consisting of a top cover, a body, and a bottom. The top cover, located at the top of the body, can be removed and is primarily used to prevent experimental organisms from escaping.

[0035] In some embodiments, the bottom of the barrel has a mounting hole communicating with the interior of the barrel body. The gas distribution structure is installed in the mounting hole by means of threaded insertion, bayonet connection, or bolt fixing; the gas distribution structure is a porous disc, a porous tube, or an integrated tube bundle structure. An integrated tube bundle is a bundle of multiple thin tubes with the same aperture integrated together. For example... Figure 5 As shown, in this embodiment, the air distribution structure is a porous disc. Multiple mounting holes are evenly distributed at the bottom of the barrel, and the bottom of the barrel is sealed and fixedly connected to the outer edge of the bottom of the barrel body.

[0036] In some embodiments, the aeration structure has various specifications, with different specifications having different numbers of air passages and / or different inner diameters. Different specifications of the aeration structure can be replaced as needed to control the number and / or size of bubbles. Since the size and number of bubbles affect hydrodynamic conditions, and further affect the diffusion rate of pollutants, changing the aeration structure allows for the study of the impact of changes in bubble size and number on water conditions and organisms under the same input of gaseous pollutants.

[0037] In some embodiments, the gaseous pollutant supply source is connected to the gas distribution structure via a gaseous pollutant delivery pipeline, and a first pressure reducing valve 8 is provided on the gaseous pollutant delivery pipeline; a second pressure reducing valve 9 is provided on the oxygen supply gas delivery pipeline, and the second pressure reducing valve is located upstream of the solid pollutant storage device.

[0038] In some embodiments, the output end of the injection pump is connected to the inside of the barrel via a first pipe; a one-way valve 10 is provided on the first pipe, the oxygen supply gas delivery pipe and the gaseous pollutant delivery pipe.

[0039] In some embodiments, the monitoring system includes multiple monitoring elements 12, including one or more of a temperature sensor, pH sensor, dissolved oxygen concentration sensor, and salinity sensor. The monitoring elements are installed on the inner wall of the tank. All of the above sensors are existing sensors, capable of continuously monitoring parameters such as temperature, pH, dissolved oxygen, and salinity 24 hours a day. There are three sets of monitoring elements, evenly distributed vertically at the top, middle, and bottom of the tank, and also evenly distributed circumferentially. The sampling port is located near the monitoring position to meet the requirements of in-situ sampling and monitoring. An existing sampling valve can be installed at the sampling port for convenient sampling.

[0040] The bottom of the container has a first pipe interface 101, a second pipe interface 102, and a third pipe interface 103 on its side. One end of the first and second pipe interfaces is connected to the inside of the container body via an inlet 104 at the bottom of the container, and the other end is connected to the first pipe and the oxygen supply gas delivery pipe, respectively. One end of the third pipe interface is connected to the inside of the container body through a gas distribution structure, and the other end is connected to the gaseous pollutant delivery pipe.

[0041] In some embodiments, the maintenance system further includes a water bath thermostat and a self-cleaning component 16. The water bath thermostat controls the temperature of the water inside the tank via a water bath. The self-cleaning component is a multi-stage filtration and adsorption device located outside the culture tank and connected to the tank via a circulation pipe, used for filtering and cleaning the water. The multi-stage filtration and adsorption device uses existing adsorption devices to filter solid impurities, biological debris, and biological metabolic waste from the water, maintaining the cleanliness of the water and reducing the impact of biological metabolites on the organisms during the aquaculture process. The water bath thermostat uses existing circulating water bath facilities to maintain the water temperature of the culture water at the set temperature.

[0042] In some embodiments, the gaseous pollutant supply source is a high-pressure gas storage tank containing gaseous pollutants and carrier gas, and the liquid pollutant supply source is a liquid storage tank containing liquid pollutants.

[0043] In some embodiments, the carrier gas includes, but is not limited to, air or nitrogen. The amount of gaseous contaminants input is controlled by controlling the concentration of gaseous contaminants in the carrier gas. The solid contaminant storage device is connected to an oxygen gas delivery pipeline via a metering feeder capable of quantitatively conveying powder. In this embodiment, the gaseous contaminant is carbon dioxide, and nitrogen is used as the carrier gas.

[0044] In some embodiments, a control system is also included. The control system includes a touch screen display 15 and a central control chip. The central control chip is connected to the monitoring system, the maintenance system, and the touch screen display via electrical signals. The touch screen display is mounted on the side of the tank bottom, and the central control chip is located inside the tank bottom. The control system has functions such as touch screen operation, data display, input, search, and control. It controls the maintenance system via electrical signals and communicates with the real-time monitoring system.

[0045] This invention discloses a hydroponic biological toxicity cultivation device suitable for pollutants in gaseous, liquid, and solid forms. It can monitor parameters such as temperature, pH, dissolved oxygen, salinity, and airflow in the aquaculture water in real time. It features a convenient sampling port, enabling comprehensive monitoring of various parameters of the aquaculture water. After programming, it can conduct unattended, uninterrupted toxicity experiments, significantly reducing manual detection. It can continuously monitor parameters such as temperature, pH, dissolved oxygen, and salinity 24 hours a day, and can also simulate the disturbance effects of different gas leakage scenarios on the aquatic environment. Through electrical signals, this invention can operate in series, simultaneously conducting toxicity cultivation experiments with different pollutants and different or equal concentrations.

[0046] The above embodiments are preferred embodiments of the invention, but the embodiments of the present invention are not limited to the above embodiments. Any changes, modifications, substitutions, combinations, or simplifications made without departing from the spirit and principle of the present invention shall be considered equivalent substitutions and shall be included within the protection scope of the present invention.

Claims

1. A hydroponic biological toxicity culture device, characterized in that: Includes culture tanks, monitoring systems, and maintenance systems; The culture tank consists of a bottom and a body. The body is used to hold the water for culturing the organisms, the bottom is connected to the bottom of the body, and the body is equipped with a sampling port. The monitoring system is used to monitor the state parameters of the water inside the tank; The maintenance system includes a contaminant input component, which comprises an injection pump, an aeration structure, a gaseous contaminant supply source, a liquid contaminant supply source, and a solid contaminant storage tank. The gaseous contaminant supply source is connected to the interior of the tank via the aeration structure, which has multiple ventilation channels through which gaseous contaminants form bubbles and enter the water body. The liquid contaminant supply source is connected to the interior of the tank via the injection pump. An oxygen gas delivery pipeline is connected to the culture tank, and the solid contaminant storage tank is connected to the oxygen gas delivery pipeline. The solid contaminant storage tank contains powdered solid contaminants, which are carried into the water body by the airflow in the oxygen gas delivery pipeline.

2. The hydroponic biological toxicity culture device according to claim 1, characterized in that: The bottom of the barrel has an installation hole that connects to the inside of the barrel body. The air distribution structure is installed in the installation hole by means of threaded insertion, bayonet connection or bolt fixation. The gas distribution structure is a porous disk, a porous tube, or an integrated tube bundle structure.

3. The hydroponic biological toxicity culture device according to claim 1, characterized in that: The air distribution structure comes in various specifications, with different specifications having different numbers of air channels and / or different inner diameters. Different specifications of air distribution structures can be replaced according to needs to control the number and / or size of air bubbles.

4. The hydroponic biological toxicity culture device according to claim 1, characterized in that: The gaseous pollutant supply source is connected to the gas distribution structure via a gaseous pollutant delivery pipeline, which is equipped with a first pressure reducing valve; the oxygen supply gas delivery pipeline is equipped with a second pressure reducing valve, which is located upstream of the solid pollutant storage tank.

5. The hydroponic biological toxicity culture device according to claim 4, characterized in that: The output end of the injection pump is connected to the inside of the barrel via the first pipe; the first pipe, the oxygen supply gas delivery pipe and the gaseous pollutant delivery pipe are each equipped with a one-way valve.

6. The hydroponic biological toxicity culture device according to claim 1, characterized in that: The monitoring system includes multiple monitoring elements, including one or more of a temperature sensor, pH sensor, dissolved oxygen concentration sensor, and salinity sensor, which are installed on the inner wall of the tank.

7. The hydroponic biological toxicity culture device according to claim 1, characterized in that: The maintenance system also includes a water bath thermostat and a self-cleaning component. The water bath thermostat controls the temperature of the water inside the tank by means of a water bath. The self-cleaning component is a multi-stage filtration and adsorption device located outside the culture tank and connected to the tank body through a circulation pipe. It is used to filter and clean the water.

8. The hydroponic biological toxicity culture device according to claim 1, characterized in that: The gaseous pollutant supply source is a high-pressure gas storage tank containing gaseous pollutants and carrier gas, and the liquid pollutant supply source is a liquid storage tank containing liquid pollutants.

9. The hydroponic biological toxicity culture device according to claim 8, characterized in that: The carrier gas is either air or nitrogen. The amount of gaseous pollutants input is controlled by controlling the concentration of gaseous pollutants in the carrier gas. The solid pollutant storage device is connected to the oxygen gas delivery pipeline via a metering feeder that can quantitatively deliver powder.

10. A hydroponic biological toxicity culture device according to claim 1, characterized in that: It also includes a control system, which includes a touch display and a central control chip. The central control chip is connected to the monitoring system, the maintenance system and the touch display via electrical signals.

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