A pesticide composition containing metrafenone

Abstract

This invention belongs to the field of pesticide technology, specifically relating to a pesticide composition containing benzimidazole. The pesticide composition's active ingredient is a compound of benzimidazole and fluazinam or emodin methyl ether. This invention, through indoor bioactivity tests, found that the compound of benzimidazole with fluazinam or emodin methyl ether, within a certain mass ratio range, has a synergistic effect against various plant fungal diseases such as Rhizoctonia solani, Curvularia crescentis, and Sclerotium sclerotiorum, helping to improve control efficacy. This is of great significance for the research and development of pesticides for controlling diseases such as cucumber damping-off, corn curvularia leaf spot, and peanut white mold.

Description

Technical Field

[0001] This invention belongs to the field of pesticide technology, and specifically relates to a pesticide composition containing benzo[a]flufenicol. Background Technology

[0002] Benzovindiflupyr is a pyrazole-4-amide fungicide that inhibits succinate dehydrogenase. It is characterized by high efficiency and broad spectrum, primarily acting on the respiratory chain of plant pathogenic fungi by blocking ATP production, leading to fungal death. Previous research by Jian Shasha, Du Qingzhi, and Deng Liyuan found that benzvindiflupyr significantly inhibits anthracnose in sorghum, powdery mildew in wheat, leaf rust in wheat, and rice blast in rice.

[0003] Cucumber damping-off, also known as seedling death or seedling blight, is one of the major soil-borne diseases of protected vegetables. It can occur from seedling emergence to transplanting, but is more common in the mid-to-late stages of seedling growth. In northern protected areas, it is mainly caused by *Rhizoctonia solani*, *Pythium cucumeris*, and *Fusarium graminearum*. Corn curvularia leaf spot, also known as yellow spot or pseudo-eye spot, is caused by *Curvularia crescentis*, which is widespread in many corn-growing areas of China. It primarily affects corn leaves, but sometimes also leaf sheaths and husks, and the symptoms vary greatly among different varieties. Peanut white mold, also known as stem rot or white mildew, is one of the most destructive soil-borne diseases of peanuts caused by *Sclerotium sclerotiorum*. As a global plant pathogen, *Sclerotium sclerotiorum* can infect more than 600 plant species, including grain crops such as wheat and rice, cash crops such as cotton and peanuts, and ornamental plants such as chrysanthemums and lilies.

[0004] In plant disease control, long-term single-use of fungicides can easily lead to drug resistance in pathogens, resulting in decreased efficacy and increased dosage. This increases control costs, exacerbates pesticide residue pollution of soil, water, and other ecological environments, and may also affect the safety of non-target organisms. Fungicide compounding involves the rational mixing and application of two or more different types of fungicides, resulting in significant and multifaceted effects, capable of controlling various types of diseases, and reducing the amount and frequency of field applications. Furthermore, there are currently no reports of compounding flutriafol with pyrimethanil.

[0005] The information disclosed in this background section is intended only to enhance the understanding of the overall background of the invention and should not be construed as an admission or in any way implying that the information constitutes prior art known to those skilled in the art. Summary of the Invention

[0006] The purpose of this invention is to provide a pesticide composition containing benzo[a]flufenicol to overcome the problems associated with using a single fungicide to control plant diseases.

[0007] To achieve the above objectives, the present invention provides the following technical solution: A pesticide composition wherein the active ingredient of the pesticide composition is a compound of benzo[a]flufenicol and fluazinam or emodin methyl ether.

[0008] Furthermore, the mass ratio of benzo[a]fluconazole to fluazinam is 2-9:8-1.

[0009] Furthermore, the mass ratio of benzo[a]fluconazole to fluazinam is 3:7, 4:6, 6:4, or 9:1.

[0010] Furthermore, the mass ratio of the compound of benzo[a]fluconazole and rhein methyl ether is 1-9:9-1.

[0011] The present invention also provides the application of the pesticide composition in the prevention and control of crop diseases.

[0012] Furthermore, crop diseases are caused by infection with Rhizoctonia solani, Curvularia crescentis, and / or Sclerotium sclerotiorum.

[0013] Compared with the prior art, the present invention has the following beneficial effects: This invention combines benzo[a]fluconazole with fluazinam or emodin methyl ether. Indoor bioactivity tests have shown that the combination, within a certain mass ratio range, has a synergistic effect on various plant fungal diseases such as Rhizoctonia solani, Curvularia crescentis, and Sclerotium sclerotiorum, which helps to improve the control effect. This is of great significance for the research and development of agents to control diseases such as cucumber damping-off, corn curvularia leaf spot, and peanut white mold. Detailed Implementation

[0014] The technical solution of this invention patent will be clearly and completely described below. Obviously, the described embodiments are only some, not all, of the embodiments of this invention. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without inventive effort are within the scope of protection of this invention.

[0015] Example: Indoor activity test 1. Test strains Table 1 Tested strains 2. Test reagents 96% benzo[a]flufenicol technical grade, 98% fluazinam technical grade, and 98% emodin methyl ether technical grade After the test reagent is dissolved, it is diluted with 0.1% Tween-80 aqueous solution to form a single-agent stock solution. Multiple ratios are set up, and each single-agent stock solution and ratio mixture is then diluted with 0.1% Tween-80 aqueous solution to form 5 mass concentration gradients.

[0016] 3. Test Methods Add 27 mL of pre-melted PDA medium and 3 mL of drug solution to a sterile conical flask, shake well, and pour the mixture into three 9 cm diameter petri dishes to prepare drug-containing plates of the corresponding concentration. Each drug solution concentration is replicated three times, with three plates per replicate. A 0.1% Tween-80 aqueous solution is used as a blank control.

[0017] After the tested strains were propagated on PDA culture media, 5 mm diameter mycelial discs were cut from the edge of the colonies using a punch. These discs were then inoculated into the center of both the drug-containing and blank control plates, one disc per plate. The plates were then capped and incubated at 25°C. When the diameter of the blank control colonies reached approximately 2 / 3 of the plate's diameter, the colony diameter was measured using the cross-sectional method, and the inhibition rate of mycelial growth by different treatments was calculated.

[0018]

[0019] 4. Data Analysis Linear regression was performed with the logarithm of the fungicide concentration as x and the corresponding probability of mycelial growth inhibition as y to derive the virulence regression equation and the EC50 of the fungicide against the target pathogen. 50 The value was calculated, and the cotoxicity coefficient (CTC) was calculated according to Sun Yunpei's method.

[0020] 5. Measurement Results The synergistic effect of the drug was evaluated based on the calculated co-toxicity coefficient (CTC). CTC ≤ 80 indicates antagonistic effect, 80 < CTC < 120 indicates additive effect, and CTC ≥ 120 indicates synergistic effect. The results are shown in Table 2-7.

[0021] Table 2. Bioactivity test of benzo[a]fluconazole combined with fluazinam in Rhizoctonia solani. As shown in Table 2, when benzalkonium chloride and fluazinam are combined, the co-toxicity coefficient of the combination against Rhizoctonia solani is less than 80 when the mass ratio is 1:9, showing an antagonistic effect; when the mass ratio is 2-9:8-1, the co-toxicity coefficient of the combination against Rhizoctonia solani is greater than 120, showing a synergistic effect, which can improve the control effect against Rhizoctonia solani disease.

[0022] Table 3. Bioactivity test of benzo[a]fluconazole and fluazinam combined with Curvularia lunata. As shown in Table 3, when benzalkonium chloride and fluazinam are combined, the co-toxicity coefficient of the combination against Curvularia lunata is less than 80 when the mass ratio is 1:9, showing an antagonistic effect; when the mass ratio is 2-9:8-1, the co-toxicity coefficient of the combination against Curvularia lunata is greater than 120, showing a synergistic effect, which can improve the control effect against Curvularia lunata diseases.

[0023] Table 4. Bioactivity test of benzo[a]fluconazole and fluazinam in combination against *Sclerotinia sclerotiorum*. As shown in Table 4, when benzalkonium chloride and fluazinam are combined in a mass ratio of 3:7, 4:6, 6:4, or 9:1, the co-toxicity coefficient of the combination against Sclerotinia sclerotiorum is greater than 120, showing a synergistic effect and improving the control effect against Sclerotinia sclerotiorum diseases.

[0024] Table 5. Bioactivity test of benzo[a]fluconazole combined with rhein methyl ether against Rhizoctonia solani. As shown in Table 5, when benzimidazole and rhein methyl ether are combined in a mass ratio of 1-9:9-1, the co-toxicity coefficient of the combination against Rhizoctonia solani is greater than 120, showing a synergistic effect and improving the control effect against Rhizoctonia solani disease.

[0025] Table 6. Bioactivity test of benzo[a]fluconazole combined with rhein methyl ether on Curvularia crescentis As shown in Table 6, when benzalkonium chloride and rhein methyl ether are combined, the co-toxicity coefficient of the combination against Curvularia lunata is greater than 120 when the mass ratio is 1:9 or 4-8:6-2, indicating a synergistic effect and improving the control effect against Curvularia lunata disease.

[0026] Table 7. Bioactivity test of benzo[a]fluconazole combined with rhein methyl ether against *Sclerotinia sclerotiorum*. As shown in Table 7, when benzimidazole and rhein methyl ether are combined in a mass ratio of 4-8:6-2, the co-toxicity coefficient of the combination against Sclerotinia sclerotiorum is greater than 120, showing a synergistic effect and improving the control effect against Sclerotinia sclerotiorum diseases.

[0027] In summary, this invention combines benzo[a]fluconazole with fluazinam or emodin methyl ether. Indoor bioactivity tests revealed that this combination, within a certain mass ratio range, exhibits synergistic effects against various plant fungal diseases, including Rhizoctonia solani, Curvularia crescentis, and Sclerotium sclerotiorum, thus improving control efficacy. This is of great significance for the research and development of agents to control diseases such as cucumber damping-off, corn curvularia leaf spot, and peanut white mold.

Claims

1. A pesticide composition, characterized in that, The active ingredient of the pesticide composition is a compound of benzo[a]flufenicol and fluazinam or emodin methyl ether.

2. The pesticide composition according to claim 1, characterized in that, The mass ratio of benzo[a]fluconazole to fluazinam is 2-9:8-1.

3. The pesticide composition according to claim 1, characterized in that, The mass ratio of benzo[a]fluconazole to fluazinam is 3:7, 4:6, 6:4, or 9:

1.

4. The pesticide composition according to claim 1, characterized in that, The mass ratio of the compound of benzo[a]fluconazole and rhein methyl ether is 1-9:9-1.

5. The application of the pesticide composition according to claim 1 in the prevention and control of crop diseases.

6. The application according to claim 5, characterized in that, The crop diseases mentioned are caused by infection with Rhizoctonia solani, Curvularia crescentis and / or Sclerotium sclerotiorum.

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