Preparation method and application of cosmetic camel thorn extract

Abstract

This invention relates to the field of daily chemical products technology, and more particularly to a method for preparing and applying a cosmetic camel thorn extract. The method for preparing the cosmetic camel thorn extract includes: crushing camel thorns, adding an ethanol solution, performing ultrasonic extraction at least twice, combining the extracts, concentrating, and obtaining the extract. This invention, based on the distribution of active ingredients in the camel thorn extract, specifically targets and extracts the active components, thereby ensuring sufficient enrichment of active ingredients and guaranteeing the stability and reliability of the extract quality. The ultrasonic treatment and vacuum concentration processes improve production efficiency and reduce production costs. Furthermore, the extract contains a variety of active ingredients, including flavonoids, polysaccharides, and other compounds, significantly enriching the composition of the extract and enhancing the potential for product efficacy and application development.

Description

Technical Field

[0001] This invention relates to the field of daily chemical products technology, and in particular to a method for preparing and applying a camel thorn extract for cosmetic use. Background Technology

[0002] Camel thorn (Alhagi pseudalhagi Desv.) is a plant belonging to the genus Alhagi in the legume family. It is a deciduous shrub endemic to the arid desert regions of Xinjiang, possessing strong regenerative and ecological adaptability, and is widely distributed in southern and eastern Xinjiang. It is not only a sand-fixing plant and high-quality forage grass, but also a pioneer plant for improving saline-alkali soils, and a traditional Uyghur medicinal herb. The whole plant of camel thorn has the effect of clearing heat and detoxifying, and is used clinically as a single herb or in compound prescriptions to treat symptoms such as colds and fevers, damp-heat in the stomach and abdomen, and enteritis and dysentery; it is also used in folk medicine to treat rheumatism and cancer.

[0003] In recent years, with the increasing demand for in-depth development of plant-based raw materials for cosmetics, research and utilization of camel thorn have also made some progress. Studies have shown that camel thorn contains a variety of active ingredients, including flavonoids, alkaloids, aliphatic compounds, sterols, amino acids, and polysaccharides. Among them, flavonoids are one of the main active substances in camel thorn and are an important secondary component produced during plant metabolism. Flavonoids possess a variety of biological activities, including antioxidant, antiviral, anti-allergic, antibacterial, antimutagenic, antitumor, hepatoprotective, immunomodulatory, anti-inflammatory, analgesic, laxative, cardiovascular protective, and insecticidal effects.

[0004] Traditional camel thorn extraction techniques have several limitations, such as long extraction times and cumbersome processes. These factors can lead to the loss or degradation of active ingredients during extraction. Furthermore, existing methods often fail to effectively concentrate and purify the active ingredients, resulting in poor quality final products.

[0005] Therefore, there is an urgent need to develop a novel extraction method to improve the extraction rate and purity of active ingredients from camel thorn, simplify the process, reduce the types of solvents used, and achieve enrichment of active ingredients. This will enable the full utilization of camel thorn resources and the development of novel cosmetic bio-sunscreen raw materials. Simultaneously, this method should effectively maintain the stability of the active ingredients and prevent degradation during the extraction process to ensure the safety and efficacy of the final product. Summary of the Invention

[0006] In view of the technical problems in the background art, the present invention aims to provide a new method for efficiently extracting active ingredients from camel thorn and applying them to the preparation of cosmetics.

[0007] This invention provides a method for preparing camel thorn extract for cosmetic use, comprising: crushing camel thorn, adding ethanol solution, heating and ultrasonically extracting at least twice, combining the extracts, concentrating, and obtaining the extract.

[0008] Preferably, the parts of camel thorn used as raw material include the fruit, leaves, and above-ground parts.

[0009] Preferably, when the camel thorn is used as a raw material and the fruit is used, the ethanol solution is composed of ethanol and water in a volume ratio of 4:6.

[0010] Preferably, when the camel thorn is used as a raw material, the leaf or above-ground part is used, and the ethanol solution is composed of ethanol and water in a volume ratio of 6:4.

[0011] Preferably, the heating temperature is 40-55°C or 50°C.

[0012] Preferably, the single extraction time for heating and ultrasonic extraction is 0.5-3 hours.

[0013] Preferably, the ratio of crushed camel thorn to ethanol solution is 1g:(8-15)mL.

[0014] The present invention also provides a cosmetic camel thorn extract prepared according to the above preparation method.

[0015] The present invention also provides the application of the above-mentioned cosmetic camel thorn extract in the preparation of ultraviolet-absorbing, moisturizing or soothing cosmetics.

[0016] Preferably, the cosmetic also includes a concentrated extract, which is obtained by extracting calendula and chamomile as raw materials through ethanol solution (65-85wt%) and compound extractant, wherein the compound extractant is composed of ethyl acetate and chloroform in a volume ratio of 1:1-2.

[0017] Preferably, the method for preparing the concentrated extract includes: mixing 0.5-3 parts by weight of calendula and 0.5-3 parts by weight of chamomile, pulverizing to obtain pulverized material, adding 65-85wt% ethanol solution at a ratio of 1g:(5-30)mL, heating and extracting at 30-60℃ for 0.5-8 hours, filtering and concentrating, adding a composite extractant (ethyl acetate and chloroform in a volume ratio of 1:1-2) to the concentrate at a ratio of 1g:(2-10)mL, extracting for 0.5-5 hours, separating the layers, taking the composite extractant layer, concentrating and drying to obtain the final product.

[0018] Preferably, the weight ratio of camel thorn extract to concentrated extract in the cosmetic is 1:0.5-1.5 or 1:0.5-1. The extract is concentrated to 0.5-1.5 times the weight of the pulverized material.

[0019] This invention also provides the application of the camel thorn extract and concentrated extract in synergistic soothing effects. The weight ratio of the camel thorn extract and concentrated extract is 1:0.5-1.5 or 1:0.5-1.

[0020] The beneficial effects of this invention are:

[0021] The extraction method used in this invention targets the active components in camel thorn extract based on their distribution, thereby ensuring that the active components in camel thorn extract are fully enriched and guaranteeing the stability and reliability of product quality.

[0022] This invention simplifies the extraction process of camel thorn extract by using ultrasonic treatment and vacuum concentration, improves production efficiency, and reduces production costs, thus showing good prospects for industrial application.

[0023] The camel thorn extract prepared by this invention contains a variety of active ingredients, including flavonoids, polysaccharides and other compounds, which significantly enriches the composition of the extract and improves the potential for efficacy and application development of the product.

[0024] This invention uses a mixed solvent of ethanol and water to extract camel thorn, avoiding the degradation of active ingredients caused by high temperatures in traditional hot reflux extraction methods. This effectively improves the stability and content of the extract, ensuring the full utilization of camel thorn resources.

[0025] This invention employs a uniform material ratio and extraction process parameters, making it easy to scale up the production of camel thorn extract. It exhibits good repeatability and controllability, ensuring the batch stability of camel thorn extract.

[0026] Among the extractions from different parts of *Alpaca thorn*, the fruit extract had the highest content of gallic acid and polysaccharides, while the rutin content of the fruit extract and the leaf extract was similar. When the extraction solvent was a mixture of ethanol and water (volume ratio 4:6), the fruit extract had the highest gallic acid content (212.94 mg / ml), 10.1 times that of the leaf extract, exhibiting good antioxidant activity. At this point, the fruit extract also had the highest polysaccharide content (38104.65 mg / ml), 2.2 times that of the leaf extract, indicating abundant polysaccharides and excellent moisturizing and repairing effects. Therefore, the optimal extraction conditions for the *Alpaca thorn* fruit extract are: grinding the *Alpaca thorn* fruit into powder; measuring the solvent (volume ratio, ethanol:water = 4:6) at a material ratio of 1:10 (g / mL); sonicating at 50℃ for 3 times, 2 hours each time; filtering; combining the filtrates; and concentrating under reduced pressure to obtain the extract.

[0027] The camel thorn leaf extract exhibits the strongest UV absorption effect among extracts from different parts of the plant, and also has the highest rutin content. When the extraction solvent is a mixture of ethanol and water at a volume ratio of 6:4, the camel thorn leaf extract has the highest rutin content of 402.64 mg / mL, higher than the fruit extract and the aerial part extract. Its high rutin content indicates good anti-inflammatory and repairing effects. Therefore, the optimal extraction conditions for camel thorn leaf extract are: a material-to-solvent ratio of 1:10 (g / mL), ultrasonication at 50℃ for 3 times, 2 hours each time, filtration, combining the filtrates, and concentration under reduced pressure to obtain the extract.

[0028] For the extract of the aerial parts of *Alpaca thorn*, the distribution of rutin and gallic acid content followed a normal distribution with respect to the ethanol content. When the extraction solvent was a mixture of ethanol and water with a volume ratio of 6:4, the rutin content in the aerial part extract was the highest (97.19 mg / L), which was 2.6 times the lowest content (36.93 mg / L); the polysaccharide content in the aerial part extract was the highest (1306.28 mg / L), which was 2.2 times the lowest content (598.84 mg / L). Therefore, preferably, the aerial parts of *Alpaca thorn* are ground into powder, and the solvent (volume ratio, ethanol:water = 6:4) is measured at a material ratio of 1:10 (g / mL). The mixture is ultrasonicated three times at 50°C for 2 hours each time. After filtration, the filtrates are combined and concentrated under reduced pressure to obtain the extract.

[0029] The concentrated extract prepared by this invention using 65-85 wt% ethanol extraction followed by further extraction with a 1:1-2 volume ratio ethyl acetate / chloroform composite extractant exhibits excellent soothing effects and can effectively inhibit the expression of inflammatory factors. The 1:1-2 volume ratio ethyl acetate / chloroform system of this invention can efficiently extract soothing and anti-inflammatory active ingredients, and the use of both ethyl acetate and chloroform in the extraction process has a synergistic effect in enhancing the inhibitory effect of the concentrated extract on the expression of inflammatory factors.

[0030] The camel thorn extract prepared by this invention has excellent soothing effects and can effectively inhibit the expression of inflammatory factors. The camel thorn extract and fermented extract prepared by this invention exhibit synergistic effects in a weight ratio of 1:0.5-1.5, enhancing the soothing and anti-inflammatory properties. Furthermore, the soothing and anti-inflammatory effect is stronger when the weight ratio is selected between 1:0.5-1. Attached Figure Description

[0031] Figure 1 Chromatograms of a mixed standard solution of rutin and gallic acid; Figure a is the chromatogram corresponding to a detection wavelength of 255 nm, and Figure b is the chromatogram corresponding to a detection wavelength of 360 nm.

[0032] Figure 2 Standard curves of rutin, gallic acid glucose concentration and absorbance value.

[0033] Figure 3 Standard curve of glucose concentration versus absorbance value.

[0034] Figure 4 : UV-Vis absorption curves; a, b, and c are the UV-Vis absorption curves of the leaf extract, fruit extract, and aerial part extract, respectively.

[0035] Figure 5 : A graph showing the inhibition of TNF-α expression by the concentrated extract.

[0036] Figure 6 Graphs showing the inhibition of TNF-α expression levels in different groups Detailed Implementation

[0037] The preferred embodiments of the present invention will be described below with reference to specific examples. It should be understood that the preferred embodiments described herein are for illustration and explanation only and are not intended to limit the present invention.

[0038] Example 1

[0039] This embodiment mainly involves the preparation of camel thorn extract and the analysis of its active ingredients.

[0040] 1. Preparation of ethanol extracts from different parts of camel thorn and testing of active ingredients

[0041] Five parts of camel thorn (leaf, thorn, seed, pod, stem) and the above-ground parts were each taken in 5g and ground into powder. The powder was mixed with 60% ethanol-water at a ratio of 1:10 (g / mL). The mixture was sonicated three times at 25℃ and 350W for 2 hours each time. The liquids after the three sonications were collected and combined, and concentrated by rotary evaporation to remove the ethanol, yielding the extract. The extract was then diluted with 10 mL of a methanol-water mixture (1:1 volume ratio) to obtain the test sample, and the contents of rutin and gallic acid were determined.

[0042] (1) Establishment of high performance liquid chromatography separation and detection method and standard curve for rutin and gallic acid mixed solution

[0043] A TC-C18 column was used, with 0.1% formic acid water (phase A) and acetonitrile (phase B) as the mobile phases. The flow rate was 1.0 mL / min, the column temperature was 30 ℃, and the UV detection wavelength was 255 and 360 nm dual wavelengths. The gradient elution program is shown in Table 1.

[0044] Table 1: Gradient elution procedure

[0045] Weigh 100 mg of rutin and gallic acid standards and dissolve them in 10 mL of a methanol-water mixture (1:1 v / v) to obtain a 10 g / L standard stock solution, which was stored at 4°C protected from light for later use. Subsequently, the solutions were diluted with a methanol-water mixture (1:1 v / v) to obtain mixed standard solutions of rutin (0.5, 1, 5, 10, 50 mg / L) and gallic acid (0.5, 1, 5, 10, 50 mg / L) at different mass concentrations. These solutions were then filtered through a 0.22 μm microporous membrane to obtain gradient standard working solutions. HPLC analysis was performed, and standard curves for rutin and gallic acid were fitted with peak area on the ordinate and concentration on the abscissa. The results are shown in the figure. Figure 1-2 .

[0046] according to Figure 1 It can be seen that when the mixed reference solution prepared above is injected under the chromatographic conditions, both gallic acid and rutin will elute at a UV detection wavelength of 255 nm, with retention times of 6.417 and 13.477 min, respectively. When the UV detection wavelength is 360 nm, only the rutin retention peak appears at 13.477 min in the chromatogram, indicating that the chromatographic peaks of the two components are well separated, the baseline is stable, and the peak shapes are basically symmetrical.

[0047] according to Figure 2 It can be seen that when the standard curves for gallic acid and rutin were established by selecting 255 nm, the two substances showed a good relationship within their respective linear ranges.

[0048] (2) Test the content of rutin and gallic acid in extracts from different parts and aerial parts of camel thorn.

[0049] The results of rutin and gallic acid content in the extract are shown in Table 2.

[0050] Table 2: Content of rutin and gallic acid in extracts from different parts and aerial components of camel thorn.

[0051] According to Table 2, the contents of rutin and gallic acid varied significantly among different parts of the camel thorn plant. The rutin content, from highest to lowest, was in the order of leaves, pods, thorns, seeds, and stems; the rutin content in the leaves, thorns, and pods (179.66–211.12 mg / L) was approximately 30–53 times higher than that in the seeds and stems (5.45–6.32 mg / L). The gallic acid content, from highest to lowest, was in the order of seeds, pods, thorns, leaves, and stems; the seeds had the highest gallic acid content (20.04 mg / L), which was four times higher than that in the stems (5.34 mg / L), which had the lowest content.

[0052] In actual production, the leaves and spines of *Alpaca chinensis* are alternate, making them difficult to separate; separating the pods and seeds of *Alpaca chinensis* is also costly. Table 2 shows that the contents of rutin and gallic acid in the extracts of *Alpaca chinensis* spines and leaves are relatively similar, suggesting they could be combined as a single extract for *Alpaca chinensis* spine leaves. Similarly, the contents of rutin and gallic acid in the extracts of *Alpaca chinensis* pods and seeds are also relatively similar, suggesting they could be combined as a single extract for *Alpaca chinensis* fruit. The stem has the lowest content of active ingredients and is therefore excluded as an extraction site. Subsequent experiments will focus on optimizing the process using leaf extracts, fruit extracts, and the aerial parts of *Alpaca chinensis*, and determining the content of relevant active ingredients.

[0053] 2. Optimization of extraction process and testing of rutin and gallic acid content in the extract.

[0054] Based on the above results, 5 g each of thorn leaf extract, fruit extract and aerial part extract were selected and the process was optimized. The mixing ratio of different extraction solvents and the extraction temperature were optimized respectively. The detailed extraction process conditions are shown in Table 3.

[0055] Table 3: Extraction Process Conditions

[0056] After the above extraction process, the extract was diluted with 10 mL of a mixed solvent of methanol and water (volume ratio 1:1) as a sample, and the contents of rutin and gallic acid were determined. The results are shown in Table 4.

[0057] Table 4: Content of rutin and gallic acid in samples from different extraction processes

[0058] As shown in Table 4, the content distribution of rutin and gallic acid in the *Nelumbo nucifera* extract followed a normal distribution with respect to the ethanol content in the extraction solvent. The highest contents of rutin and gallic acid were observed when the volume ratio of ethanol to water in the extraction solvent was 6:4, reaching 402.64 mg / L and 21.16 mg / L, respectively. Under different ethanol-to-water solvent ratios, the highest rutin content (402.64 mg / L) was twice that of the lowest (208.91 mg / L), and the highest gallic acid content (21.19 mg / L) was 2.4 times that of the lowest (8.75 mg / L).

[0059] The distribution of rutin content in the fruit extract showed a normal distribution with the change in ethanol content in the extraction solvent. When the volume ratio of ethanol to water in the extraction solvent was 6:4, the rutin content in the fruit extract was the highest (388.41 mg / L), which was 10 times the lowest content (38.80 mg / L). The distribution of gallic acid content showed multiple normal distribution peaks with the change in ethanol content. When the volume ratio of ethanol to water in the extraction solvent was 8:2 and 2:8, the content reached the highest (260.43 and 260.68 mg / L), which were both 23.50 times the lowest content (11.08 mg / L).

[0060] For the extracts from the aerial parts, the distribution of rutin and gallic acid contents followed a normal distribution with respect to the ethanol content in the extraction solvent. When the volume ratio of ethanol to water in the extraction solvent was 6:4, the rutin content in the aerial part extract was the highest (97.19 mg / L), which was 2.6 times the lowest content (36.93 mg / L). When the volume ratio of ethanol to water in the extraction solvent was 2:8, the gallic acid content in the aerial part extract was the highest (20.93 mg / L), which was 5.75 times the lowest content (3.64 mg / L).

[0061] 3. Testing of polysaccharide content in extracts obtained from different extraction processes

[0062] (1) Establishing a glucose standard curve

[0063] Different concentrations of glucose solutions (20, 40, 60, 80, 100 mg / L) and 5% phenol solutions were prepared using ultrapure water. Equal volumes of the glucose and phenol solutions were mixed, and a suitable amount of concentrated sulfuric acid was added. The mixture was heated in a boiling water bath for 100 min, then cooled to room temperature. The absorbance at 485 nm was measured. A standard curve was fitted with absorbance as the ordinate and glucose concentration as the abscissa. (See figure). Figure 3 .according to Figure 3 It can be seen that the standard curve of glucose concentration and absorbance value has a good relationship within the linear range.

[0064] (2) Test the polysaccharide content in the extract

[0065] The sample obtained in Chapter 2 was further diluted 200 times with an ethanol-water solution (ethanol:water volume ratio 6:4). An appropriate amount was then added to a centrifuge tube, and an appropriate amount of anhydrous ethanol was added to make the total ethanol volume of the system reach 80% for polysaccharide precipitation. The mixture was allowed to stand overnight at 4°C, and centrifuged at 12000 g for 25 min the next day. The supernatant was discarded. Subsequently, the precipitate was washed three times with 80% ethanol, centrifuged at 12000 g for 10 min each time. Finally, the precipitate was air-dried to remove ethanol, reconstituted with ultrapure water, and the absorbance was measured at 485 nm. The polysaccharide content in different parts of the camel thorn extract was calculated according to the standard curve, as shown in Table 5.

[0066] Table 5: Polysaccharide content in different samples under different extraction processes

[0067] As shown in Table 5, the polysaccharide content generally showed a trend of fruit extract > thorn leaf extract > aerial part extract. For the fruit extract, the highest polysaccharide content (38104.65 mg / L) was obtained when the extraction solvent was ethanol:water at a ratio of 4:6. The highest polysaccharide content (17174.42 mg / L) was obtained when the extraction solvent was ethanol:water at a ratio of 6:4.

[0068] 4. Testing of the UV absorption properties of extracts obtained by different extraction processes

[0069] The sample extracted in Chapter 2 was further diluted 400 times with an ethanol-water solution (ethanol:water volume ratio 6:4), and then its spectral absorption in the ultraviolet and blue light wavelength range (280~600 nm) was measured. The results are shown in [Figure 1]. Figure 4 .

[0070] according to Figure 4 It is known that all camel thorn extracts exhibit good absorption in the ultraviolet region (280~400 nm). For the UVB region (280~320 nm), both the leaf extract and the fruit pod mixture show good absorption. For the UVA region (320~400 nm), the leaf extract demonstrates stronger absorption than the fruit extract, possibly because other UVA-absorbing substances in the leaf extract, such as chlorophyll, also contribute to UVA absorption. Camel thorn contains flavonoids and flavonoid organic acids, represented by rutin and gallic acid. Its molecular structure is primarily composed of benzene rings, which undergo n-π and p-π electronic transitions upon ultraviolet irradiation.

[0071] Example 2

[0072] 1. Preparation and testing of concentrated extracts

[0073] Concentrated Extract 1:

[0074] 10g of calendula and 15g of chamomile were mixed and pulverized to obtain a powder. 1g of the powder was added to 15mL of 65wt% ethanol solution, and the mixture was heated at 50℃ and 150rpm for 3.5 hours. The mixture was then filtered and concentrated under reduced pressure at 55℃ to 1.05 times the weight of the powder, yielding a concentrate. A composite extractant (ethyl acetate and chloroform in a 1:1 volume ratio) was added to the concentrate at a ratio of 1g of concentrate to 4.5mL, and the mixture was extracted at room temperature for 2.5 hours. The mixture was then allowed to stand at room temperature for 0.5 hours to separate into layers. The composite extractant layer was collected, concentrated under reduced pressure to remove the extractant, and dried under vacuum at 50℃ to constant weight.

[0075] Concentrated Extract 2:

[0076] 15g of calendula and 12g of chamomile were mixed and pulverized to obtain a powder. A 70wt% ethanol solution was added to the powder at a ratio of 1g:12.5mL. The mixture was heated and extracted at 55℃ and 150rpm for 4 hours. After filtration and concentration under reduced pressure at 57℃ to 1.15 times the weight of the powder, a concentrate was obtained. A composite extractant (ethyl acetate and chloroform in a 1:2 volume ratio) was added to the concentrate at a ratio of 1g:4.2mL. The mixture was extracted at room temperature for 2.8 hours, allowed to stand at room temperature for 0.5 hours to separate the layers, and the composite extractant layer was collected. The extractant was removed by concentration under reduced pressure, and the mixture was dried under vacuum at 50℃ to constant weight.

[0077] Concentrated Extract 3: The difference from Concentrated Extract 1 is that it does not undergo extraction with a compound extractant. The process is as follows:

[0078] Mix 10g of calendula and 15g of chamomile by weight and pulverize them to obtain a powder. Add 1g of the powder to 15mL of 80wt% ethanol solution and heat at 50℃ and 150rpm for 6 hours. Filter and concentrate under reduced pressure at 55℃ to 1.05 times the weight of the powder to obtain a concentrate. Dry the concentrate under vacuum at 50℃ to constant weight.

[0079] Concentrated Extract 4: The difference from Concentrated Extract 1 is that the compound extractant is replaced with cyclohexane, otherwise they are the same.

[0080] Concentrated Extract 5: The difference from Concentrated Extract 1 is that the compound extractant is replaced with ethyl acetate, otherwise they are the same.

[0081] Concentrated Extract 6: The difference from Concentrated Extract 1 is that the compound extractant is replaced with chloroform, otherwise they are the same.

[0082] The concentrated extracts 1-6 were named samples 1-6 respectively for testing their soothing effects. Specifically, the tests focused on the samples' ability to inhibit the secretion of the inflammatory factor TNF-α under lipopolysaccharide (LPS) stimulation. The tests are as follows:

[0083] Consumables: RAW264.7 cells, incubator, DMEM medium, TNF-α detection kit, lipopolysaccharide (LPS), dexamethasone sodium phosphate, and samples 1-6 above.

[0084] Drug administration: blank group (culture medium only), model group (culture medium containing 0.5 μg / mL LPS), positive group (culture medium containing 0.5 μg / mL LPS and 110 μg / mL dexamethasone sodium phosphate), and sample group (culture medium containing 0.5 μg / mL LPS and 0.25 wt% samples 1-6).

[0085] Test procedure: RAW264.7 cells (in the logarithmic growth active phase) were seeded into 96-well plates using culture medium, 120 μL per well, with a cell volume of 1 × 10⁻⁶ cells / well. 4 Each well was incubated at 37°C with 5% CO2 for 24 hours. The culture medium was discarded, and 120 μL of the drug was added to each of the 96 wells in each group according to the above drug administration design. The wells were then incubated at 37°C with 5% CO2 for 24 hours. After incubation, the TNF-α expression level was detected according to the instructions of the TNF-α enzyme-linked immunosorbent assay kit. Each group was repeated four times. The average TNF-α expression level for each group was obtained, and the results are shown in the table below. Figure 5 .

[0086] according to Figure 5 Tests show that the concentrated extract prepared by the present invention using 65-85wt% ethanol extraction and further using a 1:1-2 volume ratio ethyl acetate / chloroform composite extractant has excellent soothing effects and can effectively inhibit the expression of inflammatory factors.

[0087] Combination Figure 5 Tests on samples 1, 3, and 4 show that sample 3, without the use of a composite extractant, resulted in a significantly reduced anti-inflammatory effect in the concentrated extract. Sample 4, using a different extractant to replace the extraction system of this application, also showed a significant decrease in anti-inflammatory effect. Therefore, the 1:1-2 volume ratio ethyl acetate / chloroform system of this invention can efficiently extract anti-inflammatory active ingredients, resulting in an extract with excellent anti-inflammatory properties. Combined with... Figure 5 The tests on samples 1, 5, and 6 show that the amount of extractant used in samples 5-6 is the same as that used in sample 1, but only one of ethyl acetate and chloroform is used. The effect of inhibiting inflammatory factors is reduced, which indicates that the two have a synergistic effect in enhancing the anti-inflammatory effect of the extract.

[0088] 2. Synergistic effect of camel thorn extract and concentrated extract

[0089] Raw material preparation: Camel thorn extract: The aerial parts of camel thorn were ground into powder. 60% ethanol (volume concentration) was measured at a ratio of 1:10 (g / mL) and mixed with 5g of the ground powder. The mixture was sonicated three times at 25℃ and 350W for 2 hours each time. The liquid after the three sonications was collected and concentrated under reduced pressure at 55℃ to 5.53g. The concentrate was then freeze-dried to a water content of 2.37wt% to obtain the camel thorn extract. Concentrated extract: Concentrated extract 1. Samples to be tested (referred to as groups) were prepared according to the weight amounts specified in Table 6. The K value in Table 6 is the ratio (by weight) of the camel thorn extract to the concentrated extract.

[0090] Table 6: Composition of the sample groups to be tested

[0091] The ability of the above-mentioned substances or combinations to inhibit the secretion of the inflammatory factor TNF-α under lipopolysaccharide (LPS) stimulation was tested as follows:

[0092] Consumables: RAW264.7 cells, incubator, DMEM medium, TNF-α detection kit, lipopolysaccharide (LPS), dexamethasone sodium phosphate, and samples 1-6 above.

[0093] Drug administration: blank group (culture medium only), model group (culture medium containing 0.5 μg / mL LPS), positive group (culture medium containing 0.5 μg / mL LPS and 110 μg / mL dexamethasone sodium phosphate), and sample group (culture medium containing 0.5 μg / mL LPS and 0.25 wt% of group 1-10 substances).

[0094] Test procedure: RAW264.7 cells (in the logarithmic growth active phase) were seeded into 96-well plates using culture medium, 120 μL per well, with a cell volume of 1 × 10⁻⁶ cells / well. 4 Each well was incubated at 37°C with 5% CO2 for 24 hours. The culture medium was discarded, and 120 μL of the drug was added to each of the 96 wells in each group according to the above drug administration design. The wells were then incubated at 37°C with 5% CO2 for 24 hours. After incubation, the TNF-α expression level was detected according to the instructions of the TNF-α enzyme-linked immunosorbent assay kit. Each group was repeated four times. The average TNF-α expression level for each group was obtained, and the results are shown in the table below. Figure 6 (For ease of observation, Figure 6 The data for the blank group and the model group are not provided.

[0095] according to Figure 6 Tests show that the camel thorn extract prepared by this invention has excellent soothing effects and can effectively inhibit the expression of inflammatory factors.

[0096] Combination Figure 6It is evident that groups 5 (1:0.5), 6 (1:0.8), 7 (1:1), and 8 (1:1.5), which combine camel thorn extract and concentrated extract, exhibit better inhibitory effects on inflammatory factor expression than groups 1 and 2, which use camel thorn extract and concentrated extract alone. Within the aforementioned ratio range, the active ingredients of the two extracts complement each other, exerting a synergistic effect beneficial to enhancing anti-inflammation. Therefore, it can be concluded that the camel thorn extract and fermented extract prepared in this invention produce a synergistic effect in the weight ratio range of 1:0.5-1.5, enhancing anti-inflammatory performance, and the soothing effect is stronger when the weight ratio is selected between 1:0.5-1. However, when the ratios are 1:0.08, 1:0.15, and 1:10, the anti-inflammatory effects either exhibit simple additive or certain antagonistic effects.

[0097] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A method for preparing a cosmetic camel thorn extract, characterized in that, include: Crush camel thorns, add ethanol solution, and extract with ultrasound at least twice. Combine the extracts, concentrate, and obtain the extract.

2. The preparation method according to claim 1, characterized in that, Camel thorns are used as raw materials for their fruit, leaves, and above-ground parts.

3. The preparation method according to claim 1, characterized in that, When camel thorn is used as a raw material, the ethanol solution consists of ethanol and water in a volume ratio of 4:

6.

4. The preparation method according to claim 1, characterized in that, When camel thorn is used as a raw material, the leaves or above-ground parts are used. The ethanol solution is composed of ethanol and water in a volume ratio of 6:

4.

5. The preparation method according to claim 1, characterized in that, The heating temperature is 40-55℃.

6. The preparation method according to claim 1, characterized in that, The heating temperature is 50℃.

7. The preparation method according to claim 1, characterized in that, The ratio of broken camel thorn to ethanol solution is 1g:(8-15)mL.

8. A cosmetic camel thorn extract prepared by the preparation method according to any one of claims 1-7.

9. The application of the cosmetic camel thorn extract according to claim 8 in the preparation of cosmetics.

10. The application according to claim 9, characterized in that, The cosmetics also include concentrated extracts, which are obtained by extracting calendula and chamomile as raw materials through ethanol solution extraction and compound extractant extraction. The compound extractant is composed of ethyl acetate and chloroform in a volume ratio of 1:1-2.

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