A Yao bath composition for promoting blood circulation and dispelling wind, its preparation method and application
The Yao bath composition, through scientific formulation and enzymatic hydrolysis, solves the problem of unscientific formulation of Yao bath products, improves efficacy and stability, significantly improves symptoms of pelvic inflammatory disease, and provides a safe and effective treatment option.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- CONGJIANG SHENYAO HEALTH CARE PROD
- Filing Date
- 2026-04-22
- Publication Date
- 2026-06-02
AI Technical Summary
Existing Yao bath products suffer from problems such as unscientific formulation, poor reproducibility, low utilization rate of medicinal materials, insufficient release of medicinal effects, poor storage stability, and weak targeting, making it difficult to effectively treat gynecological inflammations such as pelvic inflammatory disease.
By combining medicinal herbs such as Angelica sinensis, Uncaria rhynchophylla, and Coptis chinensis with compound enzymatic hydrolysis, a Yao bath composition was prepared, containing quercetin, kaempferol, and baicalin. Enzymatic hydrolysis breaks down plant cell walls, increasing the extraction rate of active ingredients. Combined with sterilization and concentration, an anti-inflammatory and antioxidant Yao bath composition was formed.
It significantly improves the efficacy of Yao bath composition, improves pelvic inflammatory disease symptoms through multi-target and multi-pathway action, enhances treatment compliance and safety, and reduces recurrence rate.
Smart Images
Figure CN122124143A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of Yao medicine technology, and more specifically, to a Yao bath composition for promoting blood circulation and dispelling wind, its preparation method, and its application. Background Technology
[0002] Pelvic inflammatory disease (PID) is a common infection of the female upper reproductive tract, mainly including endometritis, salpingitis, tubo-ovarian abscess, and pelvic peritonitis. PID is characterized by a long course, high recurrence rate, and difficulty in treatment, seriously endangering women's reproductive health. Epidemiological surveys show that approximately 4% to 12% of women of reproductive age worldwide suffer from PID. Some patients, due to failure to receive timely or complete treatment, experience serious complications such as infertility, chronic pelvic pain, and ectopic pregnancy, significantly reducing their quality of life and causing mental health problems.
[0003] Currently, the clinical treatment of PID mainly relies on antibiotic therapy. However, problems such as antibiotic overuse, incomplete treatment, the emergence of drug-resistant strains, and adverse drug reactions are becoming increasingly prominent, leading to a persistently high relapse rate and making a complete cure difficult. Therefore, there is an urgent need to develop safe, effective, and low-relapse-rate alternative or complementary treatments.
[0004] Traditional Chinese medicine (TCM) has unique advantages in treating PID, especially under the guidance of treatment principles such as "promoting blood circulation and removing blood stasis, clearing heat and dampness, and dispelling wind and unblocking collaterals," which has led to the development of various effective compound preparations and unique therapies. Yao ethnic minority medicinal baths, as an important part of traditional Chinese medicine, are characterized by "external treatment with internal effects, and the medicinal power directly reaching the affected area," showing promising application prospects in the treatment of gynecological diseases.
[0005] Currently, although there are many types of Yao bath combination products on the market, they still have the following technical defects and shortcomings: First, traditional Yao bath formulas are mostly passed down orally among the people, lacking a systematic scientific basis for formulation and a standardized quality control system, resulting in unstable product efficacy and poor reproducibility. Traditional Yao medicinal baths generally only record the types of medicinal materials, with vague dosage ratios, making it difficult to achieve industrialized production and uniform quality control. Second, the utilization rate of medicinal materials in some existing Yao bath combinations is not high; the effective components in the medicinal materials are not fully dissolved and absorbed by the human skin, making it difficult to achieve the best therapeutic effect. Traditional Yao bath methods often involve directly decocting the raw medicinal materials, which has problems such as long decoction time and insufficient release of medicinal effects. Third, existing Yao bath products lack specificity in their effects of promoting blood circulation, dispelling wind, relaxing muscles and relieving pain. Currently, there are Yao bath products on the market that dispel wind and cold, induce sweating and relieve exterior symptoms, as well as Yao bath products that clear heat and detoxify, but there is still a lack of combinations that specifically target the pathogenesis of blood stasis, damp heat, and meridian blockage, and can promote blood circulation, remove blood stasis, clear heat and dampness, dispel wind and unblock meridians. Fourth, most existing Yao bath products are packaged directly as raw medicinal powder. The powder is prone to absorbing moisture and deteriorating during storage and transportation, and the active ingredients are easily volatilized and lost, affecting the product's shelf life and effectiveness.
[0006] Therefore, developing a scientifically formulated, effective, quality-controllable, and easy-to-use Yao bath composition for activating blood circulation and dispelling wind, and its preparation method, has significant clinical value and broad market prospects. Summary of the Invention
[0007] Based on this, in order to solve one of the above-mentioned technical problems, the present invention provides a Yao bath composition for activating blood circulation and dispelling wind, its preparation method, and its application. The specific technical solution is as follows: A Yao bath composition for promoting blood circulation and dispelling wind, the Yao bath composition comprising the following raw materials in parts by weight: 10-30 parts of Polygonum aviculare, 10-20 parts of Uncaria rhynchophylla, 10-20 parts of Coptis chinensis, 10-20 parts of Polygonum multiflorum, 5-15 parts of Clematis chinensis, 5-15 parts of Cinnamomum cassia, 5-15 parts of Angelica pubescens, 5-15 parts of Eucommia ulmoides, 5-15 parts of Achyranthes bidentata, 3-10 parts of Glycyrrhiza uralensis, 3-10 parts of Commiphora myrrha, and 3-10 parts of Artemisia argyi.
[0008] Furthermore, the active ingredients of the Yao bath composition include quercetin, kaempferol, and baicalin.
[0009] In addition, the present invention also provides a method for preparing a Yao bath composition for activating blood circulation and dispelling wind, the preparation method comprising the following steps: S1. After mixing the raw materials, crush and sieve them to obtain raw material powder; S2. Add the compound enzyme and an appropriate amount of water to the raw material powder, stir evenly, and then perform enzymatic hydrolysis to obtain the pretreated Yao bath composition; S3. The pretreated Yao bath composition is concentrated and sterilized to obtain the Yao bath composition.
[0010] Furthermore, in step S1, the material is pulverized through a 20-80 mesh sieve.
[0011] Furthermore, in step S2, the amount of the compound enzyme added accounts for 0.1% to 2% of the mass of the raw material powder.
[0012] Further, in step S2, the complex enzyme is obtained by mixing cellulase, pectinase and β-glucosidase in a mass ratio of (1~3):(1~2):(1~2).
[0013] Further, in step S2, the enzymatic hydrolysis treatment is as follows: adjust the pH to 4.5~7.5, and perform enzymatic hydrolysis at 40℃~55℃ for 1h~3h.
[0014] Furthermore, in step S3, the concentration treatment is carried out at a temperature of 45℃ to 100℃ for a time of 1h to 5h.
[0015] In addition, the present invention also provides the use of the aforementioned Yao bath composition for activating blood circulation and dispelling wind in the preparation of a medicament for treating pelvic inflammatory disease, wherein the medicament may further include pharmaceutically acceptable excipients.
[0016] Compared with existing technologies, its beneficial effects include: 1. The Yao bath composition of the present invention uses *Polygonum cuspidatum*, *Uncaria rhynchophylla*, and *Nelumbo nucifera* as the principal herbs, serving as the main anti-inflammatory and analgesic agents; *Clematis chinensis*, *Cinnamomum cassia*, *Angelica pubescens*, *Commiphora myrrha*, and *Artemisia argyi* as the assistant herbs, promoting blood circulation, dispelling cold, and relieving pain; *Polygonum multiflorum*, *Eucommia ulmoides*, and *Achyranthes bidentata* as adjuvant herbs, strengthening the body's resistance and guiding the medicine downwards; and licorice as the guiding herb, harmonizing the effects of all the herbs. It can be seen that the composition follows the traditional Chinese medicine theory of "promoting blood circulation, removing blood stasis, dispelling wind, clearing heat, and promoting diuresis." The combined effects of these herbs achieve anti-inflammatory and analgesic effects and improve pelvic microcirculation. Applied to the preparation of drugs for treating pelvic inflammatory disease, it provides a new external Chinese medicine solution for gynecological inflammation, showing significant therapeutic effects on pelvic inflammatory disease and other gynecological inflammations, effectively improving symptoms such as pain and inflammation caused by pelvic inflammatory disease.
[0017] 2. This invention utilizes compound enzymatic hydrolysis to disrupt plant cell walls, creating microcracks and pores on the surface, significantly improving its dispersibility in water. The synergistic effect of the process helps enhance the application performance of the Yao bath composition. During water extraction, the extraction rate of core active ingredients such as quercetin, kaempferol, and baicalin is significantly increased, exerting multiple pharmacological effects including anti-inflammatory, antioxidant, and antibacterial properties. This helps enhance the composition's effects of promoting blood circulation, clearing heat and dampness, dispelling wind and unblocking meridians, and anti-inflammatory and analgesic properties, thereby significantly improving the efficacy of the Yao bath composition.
[0018] 3. The core active ingredients in this invention, namely quercetin, kaempferol, and baicalein, can significantly inhibit NO release and synergistically act on multiple key targets of pelvic inflammatory disease, such as IL-6, IL-1β, TNF-α, PTGS2, and ESR1. Furthermore, by regulating signaling pathways such as AGE-RAGE and PI3K-Akt, it exerts therapeutic effects from multiple components, multiple targets, and multiple pathways. The pharmacological targets are clear, resulting in better efficacy.
[0019] 4. The Yao bath composition of the present invention can be used directly for bathing or prepared into a topical preparation. It is absorbed through the skin and avoids the first-pass effect of the liver. The raw materials are all natural plants, and the medicinal properties are harmonized with licorice and other ingredients. It has few toxic side effects, is safe to use, and the medicinal bath is simple to use and can be done at home, which significantly improves treatment compliance. Attached Figure Description
[0020] The invention will be further understood from the following description taken in conjunction with the accompanying drawings. The components in the drawings are not necessarily drawn to scale, but rather the emphasis is on illustrating the principles of the embodiments. In different views, the same reference numerals designate corresponding parts.
[0021] Figure 1 This refers to the binding conformation of the core active ingredient and core target of the Yao bath composition for activating blood circulation and dispelling wind prepared in Example 1 of the present invention. Figure 2 This is a schematic diagram illustrating the effects of the core active ingredients quercetin, kaempferol, and baicalin of the Yao bath composition for activating blood circulation and dispelling wind prepared in Example 1 of the present invention on the activity of inflammatory cells and the production of nitric oxide, respectively. Figure 3 This diagram illustrates the effect of the core active ingredients quercetin, kaempferol, and baicalin of the Yao bath composition for activating blood circulation and dispelling wind prepared in Example 1 of this invention on the release of cytokines in lipopolysaccharide-induced macrophages. Detailed Implementation
[0022] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to its embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and do not limit the scope of protection of the invention.
[0023] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to limit the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.
[0024] An embodiment of the present invention provides a Yao bath composition for promoting blood circulation and dispelling wind, the Yao bath composition comprising the following raw materials in parts by weight: 10-30 parts of Polygonum aviculare, 10-20 parts of Uncaria rhynchophylla, 10-20 parts of Coptis chinensis, 10-20 parts of Polygonum multiflorum, 5-15 parts of Clematis chinensis, 5-15 parts of Cinnamomum cassia, 5-15 parts of Angelica pubescens, 5-15 parts of Eucommia ulmoides, 5-15 parts of Achyranthes bidentata, 3-10 parts of Glycyrrhiza uralensis, 3-10 parts of Commiphora myrrha, and 3-10 parts of Artemisia argyi.
[0025] In one embodiment, the active ingredients of the Yao bath composition include quercetin, kaempferol, and baicalin.
[0026] In one embodiment, the chemical structural formula of the quercetin is: .
[0027] In one embodiment, the chemical structural formula of the kaempferol is: .
[0028] In one embodiment, the chemical structural formula of baicalin is: .
[0029] In addition, the present invention also provides a method for preparing a Yao bath composition for activating blood circulation and dispelling wind, the preparation method comprising the following steps: S1. After mixing the raw materials, crush and sieve them to obtain raw material powder; S2. Add the compound enzyme and an appropriate amount of water to the raw material powder, stir evenly, and then perform enzymatic hydrolysis to obtain the pretreated Yao bath composition; S3. The pretreated Yao bath composition is concentrated and sterilized to obtain the Yao bath composition.
[0030] In one embodiment, in step S1, the material is crushed through a 20-80 mesh sieve.
[0031] In one embodiment, in step S2, the amount of the compound enzyme added accounts for 0.1% to 2% of the mass of the raw material powder.
[0032] In one embodiment, in step S2, the complex enzyme is obtained by mixing cellulase, pectinase and β-glucosidase in a mass ratio of (1~3):(1~2):(1~2).
[0033] In one embodiment, in step S2, the enzymatic hydrolysis is performed by adjusting the pH to 4.5-7.5 and hydrolyzing at 40°C-55°C for 1-3 hours.
[0034] In one embodiment, in step S2, 6 to 12 times the volume of water of the raw material powder is added.
[0035] In one embodiment, in step S3, the drying process is carried out at a temperature of 45°C to 100°C for 1 hour to 5 hours. This invention employs low-temperature drying, which maximizes the preservation of the natural active structure while maintaining the sensory qualities of the product, including its color and odor.
[0036] In addition, the present invention also provides the use of the aforementioned Yao bath composition for activating blood circulation and dispelling wind in the preparation of a medicament for treating pelvic inflammatory disease, wherein the medicament may further include pharmaceutically acceptable excipients.
[0037] The Yao bath composition provided in the above scheme exerts its therapeutic effect on pelvic inflammatory disease through multiple components, multiple targets, and multiple pathways. Its pharmacological targets are clear, and its effects are superior.
[0038] The implementation schemes of the present invention will now be described in detail with reference to specific embodiments.
[0039] Example 1: A method for preparing a Yao bath composition for promoting blood circulation and dispelling wind includes the following steps: S1. According to the weight parts, 22 parts of Poria cocos, 15 parts of Uncaria rhynchophylla, 13 parts of Coptis chinensis, 15 parts of Polygonum multiflorum, 12 parts of Clematis chinensis, 10 parts of Cinnamomum cassia, 10 parts of Angelica pubescens, 9 parts of Eucommia ulmoides, 11 parts of Achyranthes bidentata, 7 parts of Glycyrrhiza uralensis, 7 parts of Commiphora myrrha and 8 parts of Artemisia argyi are mixed and then pulverized through a 20-mesh sieve to obtain raw material powder. S2. Add 2% of the compound enzyme by mass of the raw material powder to the raw material powder, add 10 times the volume of water, stir evenly, adjust the pH to 5.5, and enzymatically hydrolyze at 50°C for 2 hours to obtain the pretreated Yao bath composition. The complex enzyme is obtained by mixing cellulase, pectinase and β-glucosidase in a mass ratio of 3:1:1. S3. The pretreated Yao bath composition is concentrated at 85°C for 3 hours and then sterilized to obtain the Yao bath composition.
[0040] Example 2: A method for preparing a Yao bath composition for promoting blood circulation and dispelling wind includes the following steps: S1. According to the weight parts, mix 23 parts of Polygonum aviculare, 16 parts of Uncaria rhynchophylla, 12 parts of Coptis chinensis, 14 parts of Polygonum multiflorum, 13 parts of Clematis chinensis, 11 parts of Cinnamomum cassia, 9 parts of Angelica pubescens, 11 parts of Eucommia ulmoides, 10 parts of Achyranthes bidentata, 8 parts of Glycyrrhiza uralensis, 9 parts of Commiphora myrrha and 9 parts of Artemisia argyi, and then pulverize them through a 20-mesh sieve to obtain the raw material powder. S2. Add 2% of the compound enzyme by mass of the raw material powder to the raw material powder, add 10 times the volume of water, stir evenly, adjust the pH to 5.5, and enzymatically hydrolyze at 50°C for 2 hours to obtain the pretreated Yao bath composition. The complex enzyme is obtained by mixing cellulase, pectinase and β-glucosidase in a mass ratio of 3:1:1. S3. The pretreated Yao bath composition is concentrated at 85°C for 3 hours and then sterilized to obtain the Yao bath composition.
[0041] Example 3: A method for preparing a Yao bath composition for promoting blood circulation and dispelling wind includes the following steps: S1. According to the weight parts, mix 25 parts of Polygonum cuspidatum, 15 parts of Uncaria rhynchophylla, 10 parts of Coptis chinensis, 13 parts of Polygonum multiflorum, 12 parts of Clematis chinensis, 12 parts of Cinnamomum cassia, 10 parts of Angelica pubescens, 12 parts of Eucommia ulmoides, 11 parts of Achyranthes bidentata, 10 parts of Glycyrrhiza uralensis, 10 parts of Commiphora myrrha and 10 parts of Artemisia argyi, and then pulverize them through a 20-mesh sieve to obtain raw material powder. S2. Add 2% of the compound enzyme by mass of the raw material powder to the raw material powder, add 10 times the volume of water, stir evenly, adjust the pH to 5.5, and enzymatically hydrolyze at 50°C for 2 hours to obtain the pretreated Yao bath composition. The complex enzyme is obtained by mixing cellulase, pectinase and β-glucosidase in a mass ratio of 3:1:1. S3. The pretreated Yao bath composition is concentrated at 85°C for 3 hours and then sterilized to obtain the Yao bath composition.
[0042] Comparative Example 1: The difference between Comparative Example 1 and Example 3 is that the raw materials used to prepare the Yao bath composition in Comparative Example 1 are different, while the rest are the same as in Example 3. The Yao bath composition in Comparative Example 1 includes the following raw materials in parts by weight: 40 parts of Angelica sinensis, 18 parts of Polygonum multiflorum, 17 parts of Clematis chinensis, 15 parts of Cinnamomum cassia, 16 parts of Eucommia ulmoides, 12 parts of Achyranthes bidentata, 10 parts of Glycyrrhiza uralensis, 10 parts of Commiphora myrrha, and 10 parts of Artemisia argyi.
[0043] Comparative Example 2: The difference between Comparative Example 2 and Example 3 is that a single cellulase was added for enzymatic hydrolysis in step S2 of the preparation method of Comparative Example 2 (i.e., no pectinase and β-glucosidase were added), while the rest was the same as Example 3.
[0044] Comparative Example 3: The difference between Comparative Example 3 and Example 3 is that Comparative Example 3 did not undergo enzymatic hydrolysis; that is, Comparative Example 3 only underwent the pulverization process in step S1.
[0045] I. To verify the leaching effect of the active ingredients in the Yao bath composition, the following application examples were made.
[0046] The Yao bath compositions prepared in Examples 1-3 and the Yao bath compositions prepared in Comparative Examples 1-3 were added to water and decocted. The amount of water added was 10 times the total mass of the Yao bath compositions. The extraction time was 1 hour. After filtration, the filtrate was collected, cooled, and concentrated under reduced pressure to dryness. The filtrate was dissolved in methanol and diluted to 50 mL. The solution was then filtered through a 0.22 μm microporous membrane to obtain the test solution.
[0047] a. The contents of quercetin, kaempferol, and baicalein were simultaneously determined by high performance liquid chromatography (HPLC). The results of the determination of the contents of the three active ingredients in the test solution (mg / g Yao bath composition) are shown in Table 1 below.
[0048] Table 1: Content of Core Active Ingredients
[0049] Analysis of the data in Table 1 shows that, through component optimization, this invention achieves higher levels of effective active ingredients. Combined with process optimization, the combined enzymatic hydrolysis method (cellulase + pectinase + β-glucosidase) significantly disrupts plant cell walls and cellulose crystal zones, promoting the release and dissolution of quercetin, kaempferol, and baicalin, thus ensuring the efficacy of the Yao bath composition. Compared to Example 3, Comparative Example 1, with its altered formulation, resulted in a weaker overall active ingredient base. Although the same enzymatic hydrolysis process was used, the overall effective ingredient content was lower than in Example 3. Comparative Example 2, employing a single enzyme for hydrolysis, resulted in incomplete cell wall disruption, limiting the release of effective ingredients. Comparative Example 3, without enzymatic hydrolysis, showed significantly lower dissolution of effective ingredients compared to Example 3. This demonstrates that the synergistic optimization of the formulation and process in this invention helps improve the efficacy of the Yao bath composition.
[0050] b. In order to verify the effect of the core active ingredients of the Yao bath composition on pelvic inflammatory disease, the following experiments were also conducted.
[0051] The core active ingredients of the Yao bath composition for activating blood circulation and dispelling wind in this invention—quercetin, kaempferol, and baicalin—were used as small molecule ligands, and the core targets PTGS2, ESR1, and AR were considered as protein receptors. The results are shown in Table 2.
[0052] Table 2: Free binding energy of core components to protein receptors
[0053] Analysis of the data in Table 2 shows that the free binding energies of the core active ingredients of the Yao bath composition for activating blood circulation and dispelling wind in this invention to the protein receptors of PTGS2, ESR1, and AR are all between -9.0 and -6.55 kcal / mol, indicating that quercetin, kaempferol, and baicalin can all bind stably to the protein receptors of PTGS2, ESR1, and AR.
[0054] in addition, Figure 1 This describes the binding conformation of the core active ingredient and core target of the Yao bath composition for invigorating blood circulation and dispelling wind in this invention. From Figure 1 As can be seen from the above, the core active ingredient of the Yao bath composition for activating blood circulation and dispelling wind in this invention has a stable binding with the target.
[0055] II. Cellular Experiment Verification a. Cell culture: RAW264.7 cells (mouse macrophage line) were cultured in DMEM complete medium containing 10% fetal bovine serum, 100 U / ml penicillin G and 100 μg / ml streptomycin, and incubated at 37°C in an incubator containing 5% CO2.
[0056] b. CCK-8 assay for cell viability: RAW264.7 cells were cultured at 8 × 10⁻⁶ cells / mL. 4 Cells were seeded at a density of [number] cells / mL in 96-well plates and stimulated with lipopolysaccharide and different concentrations of compounds. After 24 hours of incubation, CCK-8 reagent was added in the dark, and the cells were gently mixed and incubated on a constant-temperature shaker for approximately 3 hours. The optical density was then measured at 450 nm using a microplate reader (Bio-Rad Laboratories, USA). Each experiment was repeated at least three times.
[0057] c. Quantification of cytokines by enzyme-linked immunosorbent assay (ELISA): RAW264.7 macrophages were subjected to an ELISA assay at a concentration of 8 × 10⁻⁶ cells / mL. 4 Cells were seeded per well in 96-well plates and incubated for 24 h. The experiment included a control group, a lipopolysaccharide (LPS) group (model group), LPS + quercetin groups (10, 20, 40 μM), LPS + kaempferol groups (12.5, 25, 50 μM), and LPS + baicalein groups (12.5, 25, 50 μM). After 24 h of drug treatment, the supernatant of the culture medium was collected and centrifuged at 3000 rpm for 20 min. The expression levels of tumor necrosis factor-α, interleukin-6, interleukin-1β, prostaglandin intraperoxidase 2, and estrogen receptor 1 in the culture medium were detected using the appropriate enzyme-linked immunosorbent assay (ELISA) kit according to the product instructions. Each experiment was repeated at least three times.
[0058] d. NO detection in culture medium: After RAW264.7 macrophages were treated with lipopolysaccharide and the drug for 24 h, 50 μL of cell supernatant was aspirated from each well for later use. The NO content in the cell supernatant of each group was measured using a NO assay kit (Nanjing Jiancheng Biotechnology Institute, China) according to the instructions, via the Gliese reagent reaction. Each experiment was repeated at least three times.
[0059] Results processing: Experimental results are expressed as mean ± standard deviation. One-way ANOVA was used for comparison of multiple groups of data. A p-value less than 0.05 was considered statistically significant.
[0060] result: Figure 2 The effects of the core active ingredients quercetin, kaempferol, and baicalein in the Yao bath composition for invigorating blood circulation and dispelling wind of this invention on the activity of inflammatory cells and the production of nitric oxide. Specifically, A represents the effect of quercetin, kaempferol, and baicalein on the activity of lipopolysaccharide-induced macrophages. B represents the effect of quercetin, kaempferol, and baicalein on the production of nitric oxide in lipopolysaccharide-induced macrophages.
[0061] from Figure 2 As can be seen from A in the diagram, quercetin, kaempferol, and baicalein are the core active ingredients of the Yao bath composition of this invention, and can serve as the main active compounds for treating female pelvic inflammatory disease. To determine whether quercetin, kaempferol, and baicalein have toxic side effects on RAW264.7 cells, CCK8 cell viability assays were performed. The results showed that certain concentrations of quercetin (10-40 μM), kaempferol (12.5-50 μM), and baicalein (12.5-50 μM) had no toxic side effects on RAW264.7 cells (P > 0.05). Furthermore, LPS induction of RAW264.7 cells did not inhibit cell proliferation (P > 0.05).
[0062] from Figure 2 Figure B shows that macrophages untreated with lipopolysaccharide (LPS) secreted or produced very low levels of NO. However, LPS-stimulated macrophages showed a significant increase in NO production (p<0.01). Different concentrations of quercetin, kaempferol, and baicalein (10–20 μM quercetin, 12.5–50 μM kaempferol, and 12.5–50 μM baicalein) significantly inhibited nitric oxide release in a concentration-dependent manner (p<0.01). This inhibition of NO production was not due to cytotoxicity, indicating that quercetin, kaempferol, and baicalein have a strong inhibitory effect on macrophage NO production.
[0063] e. Cytokine Expression Results: Pharmacological analysis showed that IL-1β, IL6, TNF-α, PTGS2, and ESR1 were the main targets for the treatment of pelvic inflammatory disease. To further investigate how quercetin, kaempferol, and baicalein affect the expression of these targets, the protein expression levels of IL-1β, IL6, TNF-α, PTGS2, and ESR1 in each RAW 264.7 group were further examined.
[0064] Figure 3 The effects of quercetin, kaempferol, and baicalein on lipopolysaccharide-induced cytokine release in macrophages were investigated (## indicates P < 0.01 compared to the control group; * indicates P < 0.05 compared to the lipopolysaccharide group; ** indicates P < 0.01 compared to the lipopolysaccharide group). Figure 3The results showed that, compared with the control group, the expression of IL-1β, IL6, TNFα, PTGS2 and ESR1 in RAW 264.7 induced by LPS was significantly increased (P<0.01), while treatment with different concentrations of quercetin, kaempferol and baicalein significantly inhibited the high expression of inflammatory factors in the LPS group.
[0065] In summary, the causes of pelvic inflammatory disease (PID) are diverse, including: (1) sexually transmitted pathogens, such as Neisseria gonorrhoeae and Chlamydia trachomatis; (2) respiratory or intestinal pathogens infecting the female reproductive tract; and (3) bacteria and mycoplasma, as part of the vaginal microbiome, can invade the upper reproductive tract and cause inflammation. Furthermore, excessive activation of the immune system may lead to an overproduction of inflammatory factors. This excessive inflammatory response can exacerbate local infection damage and may contribute to the development of PID in the long-term, recurrent process. Therefore, inflammation is considered a key factor in the occurrence and development of PID. In this process, pro-inflammatory mediators such as adhesion molecules, chemokines, and inflammatory factors play a central role in leukocyte recruitment and the inflammatory cascade, exacerbating the inflammatory response. Therefore, inhibiting the release of pro-inflammatory mediators has become an important strategy for treating PID. Lipopolysaccharide (LPS), as a component of the outer membrane of Gram-negative bacteria, can induce peritoneal macrophage infiltration in mice and activate macrophage inflammatory responses. Targeting the LPS-induced inflammatory response in macrophages may be another potential therapeutic direction for improving PID. This further illustrates that the core active ingredients of the Yao bath composition for activating blood circulation and dispelling wind in this invention can be used as a drug for treating pelvic inflammatory disease through a multi-target mechanism of action. Specifically, quercetin inhibits lipopolysaccharide-induced macrophage migration by inhibiting NO production, disrupting the F-actin cytoskeleton, and inhibiting the focal adhesion kinase-pile protein signaling pathway; kaempferol inhibits the production of prostaglandin E2 and NO induced by the inflammatory cytokines interleukin-1β, suppressing the inflammatory response, and low concentrations of kaempferol can also inhibit the phosphorylation of nuclear factor κB inhibitory protein α and nuclear factor κB p65, and block the secretion of downstream inflammatory factors; baicalin can alleviate inflammation and pathological changes associated with acute lung injury by regulating the peroxisome proliferator-activated receptor γ-mediated nuclear factor κB pathway. It can also block the NLR family Pyrin domain protein 3 / cysteine-aspartate protease-1 / quercetin D pathway in psoriasis, exerting anti-proliferative, anti-inflammatory, and anti-apoptotic effects in an M5-induced psoriasis cell model. Therefore, the Yao bath composition for activating blood circulation and dispelling wind of the present invention, through the synergistic effect of its components, mainly through active ingredients such as quercetin, kaempferol, and baicalin, acts on key target genes of pelvic inflammatory disease such as tumor necrosis factor-α, interleukin-6, interleukin-1β, prostaglandin intraperoxidase 2, and estrogen receptor 1. By regulating signaling pathways such as advanced glycation end products-advanced glycation end products receptor and phosphatidylinositol 3 kinase-protein kinase B, it can exert a significant therapeutic effect on pelvic inflammatory disease.
[0066] III. Clinical Application Trials Thirty patients with pelvic inflammatory disease (PID), aged 20-40 years, diagnosed with PID (acute or subacute, with a course of ≤3 months), were enrolled. All patients voluntarily signed informed consent forms, agreeing not to be pregnant, breastfeeding, or planning to conceive within the next 3 months during the trial period, and not to receive other PID-related treatments (antibiotics, other traditional Chinese medicines, physical therapy, etc., except for emergency treatment). Patients were randomly assigned to: an experimental treatment group of 30 patients, which was further randomly divided into three subgroups (using products prepared according to Examples 1, 2, and 3, respectively), with 10 patients in each subgroup; and a control group of 10 patients. There were no significant differences in age or other data among the groups, making them comparable.
[0067] Experimental methods: (1) The control group took a commercially available anti-inflammatory tablet according to the instructions. The course of treatment was 30 days. (2) Experimental treatment group: 50 g of the Yao bath composition for invigorating blood circulation and dispelling wind was taken each time and dispersed in warm water (about 20 L) at 40~42℃. After soaking for 30 minutes, the volunteers took a sitz bath or a full-body bath (the water level should reach at least the waist) for 20 minutes each time, once a day. Or the Yao bath composition for invigorating blood circulation and dispelling wind was decocted, cooled down, and then the volunteers took a sitz bath or a full-body bath (the water level should reach at least the waist) for 20 minutes each time, once a day. (3) Continuous use for 14 days is one course of treatment. A total of 2 courses of treatment (28 days) were observed. During the treatment period, the patients were followed up once every 7 days to record symptoms and adverse events. The recurrence was followed up by telephone or outpatient visit in the third month. (4) Efficacy criteria and treatment results: Efficacy criteria: Cured: All clinical symptoms of pelvic inflammatory disease disappeared. Improved: Clinical symptoms of pelvic inflammatory disease were relieved and the condition was controlled. Ineffective: No significant improvement or worsening of clinical symptoms of pelvic inflammatory disease. Effectiveness is calculated based on cure and improvement. Treatment statistics are shown in Table 3.
[0068] Table 3: Therapeutic Effects
[0069] The data in Table 3 demonstrate that the Yao bath composition formula for invigorating blood circulation and dispelling wind of the present invention is scientifically formulated and has been verified through long-term clinical practice. The components work synergistically to clear heat and detoxify, invigorate blood circulation, promote diuresis and reduce inflammation, thereby playing a role in treating pelvic inflammatory disease. Furthermore, through the multi-component and multi-target action, it inhibits inflammatory factors such as IL-6, TNF-α, and NO, and regulates the targets of PTGS2 and ESR1. It not only quickly relieves symptoms but also improves pelvic microcirculation, reduces the risk of recurrence, and has a more significant therapeutic effect.
[0070] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.
[0071] The embodiments described above are merely illustrative of several implementations of the present invention, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the invention patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these all fall within the protection scope of the present invention. Therefore, the protection scope of this invention patent should be determined by the appended claims.
Claims
1. A Yao bath composition for activating blood circulation and dispelling wind, characterized in that, The Yao bath composition comprises the following raw materials in parts by weight: 10-30 parts of Polygonum aviculare, 10-20 parts of Uncaria rhynchophylla, 10-20 parts of Coptis chinensis, 10-20 parts of Polygonum multiflorum, 5-15 parts of Clematis chinensis, 5-15 parts of Cinnamomum cassia, 5-15 parts of Angelica pubescens, 5-15 parts of Eucommia ulmoides, 5-15 parts of Achyranthes bidentata, 3-10 parts of Glycyrrhiza uralensis, 3-10 parts of Commiphora myrrha, and 3-10 parts of Artemisia argyi.
2. The Yao bath composition according to claim 1, characterized in that, The active ingredients of the Yao bath composition include quercetin, kaempferol, and baicalin.
3. A method for preparing a Yao bath composition for activating blood circulation and dispelling wind, characterized in that, The preparation method is used to prepare the Yao bath composition as described in claim 1 or 2, and the preparation method includes the following steps: S1. After mixing the raw materials, crush and sieve them to obtain raw material powder; S2. Add the compound enzyme and an appropriate amount of water to the raw material powder, stir evenly, and then perform enzymatic hydrolysis to obtain the pretreated Yao bath composition; S3. The pretreated Yao bath composition is concentrated and sterilized to obtain the Yao bath composition.
4. The preparation method according to claim 3, characterized in that, In step S1, the material is crushed and passed through a 20-80 mesh sieve.
5. The preparation method according to claim 3, characterized in that, In step S2, the amount of the compound enzyme added accounts for 0.1% to 2% of the mass of the raw material powder.
6. The preparation method according to claim 3, characterized in that, In step S2, the complex enzyme is obtained by mixing cellulase, pectinase and β-glucosidase in a mass ratio of (1~3):(1~2):(1~2).
7. The preparation method according to claim 3, characterized in that, In step S2, the enzymatic hydrolysis treatment is as follows: adjust the pH to 4.5~7.5 and perform enzymatic hydrolysis at 40℃~55℃ for 1h~3h.
8. The preparation method according to claim 3, characterized in that, In step S3, the concentration process is carried out at a temperature of 65℃ to 100℃ for 1 hour to 5 hours.
9. The use of a Yao bath composition for activating blood circulation and dispelling wind as described in claim 1 or 2 in the preparation of a medicament for treating pelvic inflammatory disease, wherein the medicament may further include pharmaceutically acceptable excipients.