Intestinal bone core chip and construction method and application thereof
By cascading small intestinal organoids and bone organoids in a perfusion chip, and employing a method of partitioned feeding and optimized microfluidic flow rate, the problem of stable co-culture of intestine and bone was solved, realizing material exchange and signal transmission between intestine and bone, and providing a new experimental tool and drug screening platform.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- SHANGHAI UNIV
- Filing Date
- 2026-03-18
- Publication Date
- 2026-06-02
AI Technical Summary
Existing technologies make it difficult to achieve stable tandem culture of intestinal and bone tissues within the same system, and it is also difficult to take into account the different requirements of the two types of tissues for culture medium composition and fluid conditions, thus failing to effectively regulate and characterize the quantitative ratio between intestinal and bone tissues.
By connecting small intestinal organoids and bone organoids in series using a perfusion chip, and setting induction culture media for intestinal and bone organoids separately using a zoned feeding method, and adding basal culture medium to the flow fluid as a circulation medium, the microfluidic flow rate parameters are optimized to achieve compatibility between differentiated induction conditions and circulation delivery.
It simulates the exchange of substances and signal transmission between the gut and bone, provides long-term monitoring of bone metabolism by intestinal secretions and cytokines, and constructs a multi-organ platform, providing new experimental tools for studying the regulatory mechanisms of bone metabolism and drug screening.
Smart Images
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