Preparation method and application of mesenchymal stem cells induced by 3D cell spheres combined with hypoxia and low temperature synergistic treatment
The method of preparing mesenchymal stem cells by inducing 3D cell spheres and synergistic treatment with hypoxia and low temperature has solved the problems of rapid hematopoietic reconstitution, GVHD suppression and fibrosis prevention in haploidentical bone marrow transplantation for severe aplastic anemia, and achieved high cell survival and multi-target synergistic intervention.
CN122128228APending Publication Date: 2026-06-02THE FIFTH MEDICAL CENT OF CHINESE PLA GENERAL HOSPITAL +1
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- THE FIFTH MEDICAL CENT OF CHINESE PLA GENERAL HOSPITAL
- Filing Date
- 2026-03-20
- Publication Date
- 2026-06-02
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Figure CN122128228A_ABST
Abstract
This invention provides a method for preparing mesenchymal stem cells through 3D cell spheroid induction and hypoxia-hypothermia synergistic treatment, and its application, relating to the technical fields of regenerative medicine and cell therapy. The preparation method includes: inducing and culturing mesenchymal stem cells to obtain 3D cell spheroids; and subjecting the 3D cell spheroids to hypoxia-hypothermia treatment to obtain hypoxia-hypothermia-treated 3D cell spheroids. This invention achieves a synergistic enhancement of cell stemness, anti-apoptosis, and secretory function through 3D cell spheroid induction followed by hypoxia-hypothermia treatment. When these cells are used for pre-transplantation in SAA mice, they simultaneously achieve the triple effects of "accelerated hematopoietic reconstitution, reduced incidence of GVHD, and decreased risk of fibrosis."
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