Preparation method of mesoporous adsorptive bioactive protein feed

Abstract

The present application relates to the field of feed, and discloses a preparation method of mesoporous adsorbed bioactive protein feed, characterized by comprising the following steps: material preparation: processing attapulgite by lactic acid bacteria fermentation technology to obtain biologically modified attapulgite powder; mixing: mixing the biologically modified attapulgite powder with composite algae liquid, composite powder and element additives to make feed. The present application modifies attapulgite material by lactic acid bacteria fermentation technology, greatly enhances the adsorption of attapulgite on nutrient substances, and thus adsorbs nutrient substances such as protein by biologically modified attapulgite powder, so that the feed composition is not only high in protein content but also rich in various essential trace elements, can provide balanced nutrition for animals, and shows obvious advantages in improving feed quality, helping animal healthy growth, increasing economic benefits and other aspects, which is beneficial to more effective intake of nutrient components in feed by animals.

Description

Technical Field

[0001] This invention relates to the field of feed, and more specifically to a method for preparing mesoporous adsorbent bioactive protein feed. Background Technology

[0002] In modern animal husbandry, improving the nutritional value of feed and enhancing animal gut health are crucial. Traditional feeds often have limitations in nutrient absorption and animal health promotion, primarily due to low utilization rates of feed components in the animal's digestive tract and insufficient support for gut microbiome balance. Therefore, developing novel feeds, especially those capable of effectively adsorbing and protecting bioactive proteins, has become a hot topic in feed technology research. Mesoporous materials have attracted widespread attention due to their high specific surface area and tunable pore size, characteristics that make them excellent at adsorbing and releasing bioactive molecules. Attapulgite, as a natural mineral material, possesses good adsorption properties and a unique physical structure, but its adsorption efficiency and ability to protect bioactive proteins still have room for improvement. Summary of the Invention

[0003] The present invention aims to provide a method for preparing a mesoporous adsorbent bioactive protein feed, so as to optimize the adsorption performance of attapulgite and improve the protective performance of attapulgite on bioactive proteins, thereby providing a bioactive protein feed with higher nutritional value and health benefits.

[0004] To achieve the above objectives, the present invention adopts the following technical solution: a method for preparing mesoporous adsorbent bioactive protein feed, characterized by comprising the following steps: material preparation: using lactic acid bacteria fermentation technology to process attapulgite to obtain biomodified attapulgite powder; mixing: mixing the biomodified attapulgite powder with compound algae liquid, compound powder and elemental additives to prepare feed.

[0005] The beneficial effects of this solution are as follows: By using lactic acid bacteria fermentation technology to modify attapulgite materials, the adsorption capacity of attapulgite for nutrients is greatly enhanced. Thus, through biomodified attapulgite powder, nutrients such as protein are adsorbed, resulting in feed components that are not only high in protein but also rich in a variety of essential trace elements. This provides animals with a balanced nutritional supply and demonstrates significant advantages in improving feed quality, promoting healthy animal growth, and increasing economic benefits. It also helps animals to more effectively absorb the nutrients in the feed.

[0006] Furthermore, the mixture contains 15-25 parts of bio-modified attapulgite, 25-35 parts of compound algae solution, 35-45 parts of compound powder, and 5-10 parts of elemental additives.

[0007] Furthermore, the preparation of biomodified attapulgite powder using lactic acid bacteria fermentation technology includes the following steps:

[0008] S1. Pre-treat the attapulgite powder to obtain attapulgite powder with uniform particles and sterility.

[0009] S2. Inoculate lactic acid bacteria into an enrichment medium. The lactic acid bacteria are one or more of Lactobacillus fermentum, Lactobacillus plantarum, and Enterococcus. The enrichment medium is enzymatically hydrolyzed by the lactic acid bacteria to obtain lactic acid bacteria fermentation broth. The enzymatic hydrolysis temperature is 45-55℃ and the enzymatic hydrolysis time is 24-48h.

[0010] S3. Soak the pretreated attapulgite powder in S1 in the lactic acid bacteria fermentation liquid prepared in S2. The attapulgite powder is eroded by the lactic acid bacteria fermentation liquid. The soaking and erosion temperature is 36℃~38℃ and the time is 24~48h.

[0011] S4. Filter the attapulgite powder that has been soaked and eroded in S3 and dry it to constant weight to obtain biomodified attapulgite powder.

[0012] Furthermore, the pretreatment method involves using a grinder to crush the attapulgite into a homogeneous powder, then passing it through a 200-mesh sieve to remove impurities, followed by high-pressure sterilization at 120°C for 30-60 minutes, and drying in an oven at 60-80°C for 1-2 hours.

[0013] Furthermore, the enrichment culture medium comprises 8-10g peptone, 4-6g beef extract, 3-5g yeast extract, 15-20g glucose, 1-3g dipotassium hydrogen phosphate, 1-4g triammonium citrate, 3-6g sodium acetate, 0.1-0.5g magnesium sulfate, 0.05-0.1g manganese sulfate, 10-15g agar, 1-2g Tween 80, and a pH of 6.0±0.5.

[0014] Furthermore, the compound algal solution includes Chlamydomonas, Scenedesmus, and Chlorella, with a concentration ratio of 1–3:2–4:5–10; the compound powder includes corn flour and wheat flour, with a weight ratio of 33–65:28–48; and the elemental additives include calcium chloride, magnesium chloride, zinc sulfate, and ferric chloride, with a weight ratio of 30–55:25–42:5–10:2–5.

[0015] Furthermore, in S2, the method for preparing the lactic acid bacteria fermentation broth is as follows: lactic acid bacteria are inoculated into a culture medium, including one or more of Lactobacillus fermentum, Lactobacillus plantarum, and Enterococcus. The culture medium includes 8-10g of peptone, 4-6g of beef extract, 3-5g of yeast extract, 15-20g of glucose, 1-3g of dipotassium hydrogen phosphate, 1-4g of triammonium citrate, 3-6g of sodium acetate, 0.1-0.5g of magnesium sulfate, 0.05-0.1g of manganese sulfate, 10-15g of agar, and 1-2g of Tween 80. The pH of the culture medium is 6.0±0.5.

[0016] Furthermore, the preparation and mixing process includes pretreatment, which includes S5: ultrasonic treatment of the microalgae solution using ultrasonic cell disruption technology to obtain a microalgae protein solution; S6: mixing the microalgae protein solution with bio-modified attapulgite powder to obtain a bio-modified attapulgite-microalgae protein composite material; during mixing, the pre-treated bio-modified attapulgite-microalgae protein composite material is stirred and mixed with composite powder and elemental additives, and then granulated and dried to obtain mesoporous adsorbent bioactive protein feed.

[0017] Furthermore, the ultrasonic treatment conditions are an ultrasonic power of 300-600w and a time of 15-30min; in S6, the microalgae protein solution and the biomodified attapulgite powder are mixed by stirring, filtering and drying to constant weight to obtain the biomodified attapulgite-microalgae protein composite material, and the stirring time is 4-8h.

[0018] Furthermore, during mixing, the biomodified attapulgite-microalgae protein composite material is stirred and mixed with the composite powder and elemental additives for 30-60 minutes.

[0019] Based on the above technical features, the beneficial effects of this technical solution are as follows:

[0020] This technical solution offers significant advantages in improving feed quality, ensuring animal health, and enhancing industrial economic benefits. Firstly, the high protein content of the compound algae liquid and the balanced nutrition of the compound powder work together to significantly improve the overall nutritional value of the feed. Secondly, the adsorption properties of the bio-modified attapulgite, combined with probiotics and other components, effectively regulate the balance of intestinal flora and prevent diseases such as diarrhea. Through a carefully designed combination of ingredients and a unique processing technology, the feed of this invention achieves multiple benefits—improving nutritional value while simultaneously protecting intestinal health and enhancing immunity—making it highly suitable for use in various livestock industries to improve production efficiency and product quality. Attached Figure Description

[0021] Figure 1 This is a flowchart illustrating the preparation method of the mesoporous adsorption bioactive protein feed in the examples. Detailed Implementation

[0022] The following detailed description provides further details on specific embodiments, but the embodiments of the present invention are not limited thereto. Unless otherwise specified, the technical means used in the following embodiments are conventional means well known to those skilled in the art; the experimental methods used are all conventional methods; and the materials and reagents used are all commercially available.

[0023] Example 1

[0024] A method for preparing mesoporous adsorbent bioactive protein feed, comprising a formulation, a method for preparing biomodified attapulgite, a method for preparing microalgae protein solution, and a feeding method. The formulation, by weight, includes 15-25 parts of biomodified attapulgite, 25-35 parts of composite algae solution, 35-45 parts of composite powder, and 5-10 parts of elemental additives. The composite algae solution includes Chlamydomonas, Scenedesmus, and Chlorella, with a concentration ratio of 1-3:2-4:5-10. The composite powder consists of corn flour and wheat flour, with a weight ratio of 33-65:28-48. The elemental additives include calcium chloride, magnesium chloride, zinc sulfate, and ferric chloride, with a weight ratio of 30-55:25-42:5-10:2-5.

[0025] The preparation method of biomodified attapulgite is as follows:

[0026] S1: Pre-treat the attapulgite powder by grinding it into homogeneous powder using a grinder, then passing it through a 200-mesh sieve to remove impurities, and then sterilizing it under high pressure at a temperature of 120℃ for 30-60 minutes. After sterilization, dry it in an oven at 60-80℃ for 1-2 hours.

[0027] S2: Lactic acid bacteria culture. Lactic acid bacteria are inoculated into an enriched culture medium, which includes Lactobacillus fermentum, Lactobacillus plantarum, and Enterococcus. The culture medium includes 10g peptone, 6g beef extract, 5g yeast extract, 20g glucose, 3g dipotassium hydrogen phosphate, 4g triammonium citrate, 6g sodium acetate, 0.5g magnesium sulfate, 0.1g manganese sulfate, 15g agar, and 2g Tween 80. The pH is maintained at 6.0±0.5.

[0028] S3: Add the pretreated attapulgite powder to the lactic acid bacteria fermentation liquid, soak and erode at 36℃ for 24 hours, stirring constantly during the soaking process to ensure that the attapulgite powder and the lactic acid bacteria fermentation liquid are in full contact.

[0029] S4: After soaking and erosion, the attapulgite powder in S3 is filtered and dried to constant weight to obtain bio-modified attapulgite material.

[0030] The method for preparing microalgae protein solution is as follows:

[0031] S5: The microalgae solution is ultrasonically treated using ultrasonic cell disruption technology. The ultrasonic power is 300-600w, and the time is 15-30min, thus obtaining the microalgae protein solution.

[0032] The preparation method is as follows:

[0033] S6: The lactic acid bacteria fermentation modified attapulgite obtained in step S5 is immersed in a microalgae protein solution. After stirring for 8 hours, the lactic acid bacteria fermentation modified attapulgite obtained in step S4 and the microalgae protein solution obtained in step S5 are mixed evenly. Then, the mixture is filtered and dried to constant weight to obtain a biomodified attapulgite-microalgae protein composite material.

[0034] S7: Mix the biomodified attapulgite-microalgae protein composite material, composite powder, and elemental additives for 60 minutes. Then, granulate and dry to obtain mesoporous adsorption bioactive protein feed.

[0035] The ultrasonic cell disruption conditions are as follows: power 300-600W, time 15-30min.

[0036] The mesoporous adsorption bioactive protein feed formulation prepared in this embodiment is as follows:

[0037] By weight, the mixture contains 20 parts attapulgite, 25 parts compound algae solution, 45 parts compound powder, and 10 parts elemental additives. The compound algae solution includes Chlamydomonas, Scenedesmus, and Chlorella, with a concentration ratio of 2:3:8. The compound powder consists of corn flour and wheat flour, with a weight ratio of 49:38. The elemental additives include calcium chloride, magnesium chloride, zinc sulfate, and ferric chloride, with a weight ratio of 43:34:8:4.

[0038] like Figure 1 As shown, according to the above formula, the corresponding raw materials are prepared. Attapulgite is transformed into biomodified attapulgite using a biomodified attapulgite preparation method, and microalgae protein solution is prepared using a microalgae protein solution preparation method. Subsequently, according to the material preparation method, the biomodified attapulgite and microalgae protein solution are mixed sequentially to prepare a biomodified rod-microalgae protein composite material. Finally, the composite powder, elemental additives, and the prepared biomodified rod-microalgae protein composite material are stirred and mixed, granulated, and dried to obtain mesoporous adsorption bioactive protein feed.

[0039] Example 2

[0040] A method for preparing a mesoporous adsorbent bioactive protein feed, characterized in that, by weight, it comprises 25 parts of biomodified attapulgite, 35 parts of composite algal liquid, 35 parts of composite powder, and 5 parts of elemental additives.

[0041] The compound algal solution includes Chlamydomonas, Scenedesmus, and Chlorella, with a concentration ratio of 1-3:2-4:5-10; the compound powder consists of corn flour and wheat flour, with a weight ratio of 33-65:28-48; the elemental additives include calcium chloride, magnesium chloride, zinc sulfate, and ferric chloride, with a weight ratio of 30-55:25-42:5-10:2-5.

[0042] A mesoporous adsorbent bioactive protein feed, characterized in that the preparation method of the biomodified attapulgite is as follows:

[0043] S1: Pre-treat the attapulgite powder by grinding it into homogeneous powder using a grinder, then passing it through a 200-mesh sieve to remove impurities, followed by high-pressure sterilization and drying in an oven at 60-80℃ for 1-2 hours.

[0044] S2: Lactic acid bacteria culture, which involves inoculating lactic acid bacteria into an enriched culture medium.

[0045] S3: Add the pretreated attapulgite powder to the lactic acid bacteria fermentation broth and soak and erode it under the optimal conditions for strain growth.

[0046] S4: Stir, filter, and dry to constant weight to obtain biomodified attapulgite material.

[0047] The sterilization temperature is 120℃ and the time is 30-60 minutes.

[0048] The lactic acid bacteria mentioned include Lactobacillus fermentum, Lactobacillus plantarum, and Enterococcus.

[0049] The culture nutrients include 8g peptone, 4g beef extract, 3g yeast extract, 15g glucose, 1g dipotassium hydrogen phosphate, 1g triammonium citrate, 3g sodium acetate, 0.1g magnesium sulfate, 0.05g manganese sulfate, 10g agar, 1g Tween 80, and a pH of 6.0±0.5.

[0050] The optimal growth conditions for the strain are 38°C and 48 hours.

[0051] A mesoporous adsorbent bioactive protein feed, characterized by the following preparation method:

[0052] S5: The microalgae solution is ultrasonically treated using ultrasonic cell disruption technology to obtain a microalgae protein solution.

[0053] S6: The lactic acid bacteria fermentation modified attapulgite obtained in step S5 is impregnated in a microalgae protein solution, stirred, filtered, and dried to constant weight to obtain a biomodified attapulgite-microalgae protein composite material, which serves as a mesoporous adsorbent bioactive protein feed that provides nutrition and improves the gut.

[0054] S7: Mix the biomodified attapulgite-microalgae protein composite material, composite powder, and elemental additives, stir, granulate, and dry to obtain mesoporous adsorption bioactive protein feed.

[0055] The ultrasonic cell disruption conditions are as follows: power 300-600W, time 15-30min.

[0056] The stirring time in step S6 is 4 hours.

[0057] The stirring time in step S7 is 30 minutes.

[0058] Comparative Example 1

[0059] Based on Example 1, except for the different fermentation time when the attapulgite powder was added to the lactic acid bacteria fermentation broth, all other components and preparation methods were the same as in Example 1. The fermentation time was 8 hours.

[0060] Comparative Example 2

[0061] Based on Example 1, except for the different fermentation time when the attapulgite powder was added to the lactic acid bacteria fermentation broth, all other components and preparation methods were the same as in Example 1. The fermentation time was 16 hours.

[0062] Comparative Example 3

[0063] Based on Example 1, except for the fermentation time when the attapulgite powder was added to the lactic acid bacteria fermentation broth, the other components and preparation methods were the same as in Example 1. The fermentation time was 24 hours.

[0064] Comparative Example 4

[0065] Based on Example 1, attapulgite was used instead of lactic acid bacteria modified attapulgite, and the remaining components and preparation methods were the same as in Example 1.

[0066] The effects of mesoporous adsorbent bioactive protein feeds prepared with different lactic acid fermentation times on fish growth. The results of the tests on fish fed with these feeds (Example 1 and Comparative Examples 1-4) are shown in Table 1.

[0067] Group number Feeding pH Lactic acid content % Survival rate % Average body weight / g Group 1 Comparison 7.05±0.12 - 82 589 2 groups Comparative Example 1 6.05±0.15 0.88±0.05 97 620 3 groups Comparative Example 2 5.98±0.18 1.63±0.06 99 654 4 groups Comparative Example 3 5.41±0.15 1.89±0.09 99 679 5 groups Comparative Example 4 7.45±0.13 - 86 608 6 groups Experimental Example 1 4.23±0.12 1.94±0.09 99 684

[0068] As shown in Table 1 above, compared with the control group fed pure fishmeal, the feed of this invention significantly improved the survival rate and weight of adult fish. Furthermore, when the lactic acid-attapulgite fermentation time was 24 hours, the survival rate and weight of adult fish showed a more significant improvement. Attapulgite is a hydrous magnesium-aluminate silicate clay with excellent adsorption and ion exchange capabilities. When it enters the animal intestine, it can tightly coat the intestinal mucosa, effectively protecting the intestinal mucosa and reducing contact with pathogenic bacteria and their toxins. Therefore, lactic acid-modified attapulgite can improve the survival rate of fish.

[0069] The above descriptions are merely embodiments of the present invention, and common knowledge such as specific technical solutions and / or characteristics are not described in detail here. It should be noted that those skilled in the art can make various modifications and improvements without departing from the technical solutions of the present invention, and these should also be considered within the scope of protection of the present invention. These modifications and improvements will not affect the effectiveness of the implementation of the present invention or the practicality of the patent. The scope of protection claimed in this application should be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.

Claims

1. A method for preparing a mesoporous adsorbent bioactive protein feed, characterized in that: The process includes the following steps: Material preparation: using lactic acid bacteria fermentation technology to process attapulgite to obtain bio-modified attapulgite powder; Feed preparation: mixing the bio-modified attapulgite powder with compound algae liquid, compound powder and element additives to make feed.

2. The method for preparing a mesoporous adsorbent bioactive protein feed according to claim 1, characterized in that: By weight, the composition includes 15-25 parts of biomodified attapulgite, 25-35 parts of compound algae solution, 35-45 parts of compound powder, and 5-10 parts of elemental additives.

3. The method for preparing a mesoporous adsorbent bioactive protein feed according to claim 1, characterized in that: The preparation of biomodified attapulgite powder using lactic acid bacteria fermentation technology includes the following steps: S1. Pre-treat the attapulgite powder to obtain attapulgite powder with uniform particles and sterility. S2. Inoculate lactic acid bacteria into an enrichment medium. The lactic acid bacteria are one or more of Lactobacillus fermentum, Lactobacillus plantarum, and Enterococcus. The enrichment medium is enzymatically hydrolyzed by the lactic acid bacteria to obtain lactic acid bacteria fermentation broth. The enzymatic hydrolysis temperature is 45-55℃ and the enzymatic hydrolysis time is 24-48h. S3. Soak the pretreated attapulgite powder in S1 in the lactic acid bacteria fermentation liquid prepared in S. The attapulgite powder is eroded by the lactic acid bacteria fermentation liquid. The soaking and erosion temperature is 36℃~38℃ and the time is 24~48h. S4. Filter the attapulgite powder that has been soaked and eroded in S3 and dry it to constant weight to obtain biomodified attapulgite powder.

4. The method for preparing a mesoporous adsorbent bioactive protein feed according to claim 3, characterized in that: The pretreatment method in S1 is to use a grinder to crush the attapulgite into a homogeneous powder, then pass it through a 200-mesh sieve to remove impurities, then sterilize it under high pressure at a temperature of 120℃ for 30-60 minutes, and then dry it in an oven at 60-80℃ for 1-2 hours.

5. The method for preparing a mesoporous adsorbent bioactive protein feed according to claim 3, characterized in that: The enrichment medium in S2 includes 8-10g peptone, 4-6g beef extract, 3-5g yeast extract, 15-20g glucose, 1-3g dipotassium hydrogen phosphate, 1-4g triammonium citrate, 3-6g sodium acetate, 0.1-0.5g magnesium sulfate, 0.05-0.1g manganese sulfate, 10-15g agar, and 1-2g Tween 80, with a pH of 6.0±0.

5.

6. The method for preparing a mesoporous adsorbent bioactive protein feed according to claim 3, characterized in that: The compound algal solution includes Chlamydomonas, Scenedesmus, and Chlorella, with a concentration ratio of 1–3:2–4:5–10; the compound powder includes corn flour and wheat flour, with a weight ratio of 33–65:28–48; the elemental additives include calcium chloride, magnesium chloride, zinc sulfate, and ferric chloride, with a weight ratio of 30–55:25–42:5–10:2–5.

7. The method for preparing a mesoporous adsorbent bioactive protein feed according to claim 3, characterized in that: In S2, the method for preparing lactic acid bacteria fermentation broth is as follows: lactic acid bacteria are inoculated into a culture medium, including one or more of Lactobacillus fermentum, Lactobacillus plantarum, and Enterococcus. The culture medium includes 8-10g of peptone, 4-6g of beef extract, 3-5g of yeast extract, 15-20g of glucose, 1-3g of dipotassium hydrogen phosphate, 1-4g of triammonium citrate, 3-6g of sodium acetate, 0.1-0.5g of magnesium sulfate, 0.05-0.1g of manganese sulfate, 10-15g of agar, and 1-2g of Tween 80. The pH of the culture medium is 6.0±0.

5.

8. The method for preparing a mesoporous adsorbent bioactive protein feed according to claim 3, characterized in that: The preparation process also includes the following steps: S5, ultrasonically treating the microalgae solution using ultrasonic cell disruption technology to obtain a microalgae protein solution; S6, mixing the microalgae protein solution with bio-modified attapulgite powder to obtain a bio-modified attapulgite-microalgae protein composite material; during feed preparation, the pre-treated bio-modified attapulgite-microalgae protein composite material is stirred and mixed with composite powder and elemental additives, and then granulated and dried to obtain mesoporous adsorption bioactive protein feed.

9. The method for preparing a mesoporous adsorbent bioactive protein feed according to claim 8, characterized in that: The ultrasonic treatment conditions are an ultrasonic power of 300-600w and a time of 15-30min; in S6, the microalgae protein solution and the biomodified attapulgite powder are mixed by stirring, filtering and drying to constant weight to obtain the biomodified attapulgite-microalgae protein composite material, and the stirring time is 4-8h.

10. The method for preparing a mesoporous adsorbent bioactive protein feed according to claim 9, characterized in that: During mixing, the biomodified attapulgite-microalgae protein composite material is stirred and mixed with the composite powder and elemental additives for 30-60 minutes.

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