Sea cucumber powder for promoting postoperative repair and cell regeneration, preparation method and application thereof
By employing a synergistic process of preliminary and deep extraction, and utilizing compound enzymes A and B to hydrolyze sea cucumbers, combined with low-temperature treatment, highly bioactive sea cucumber powder was prepared. This process solved the problem of insufficient retention of active ingredients in existing technologies and achieved the effects of postoperative repair and cell regeneration.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- FUJIAN DAZHONG HEALTH BIOTECHNOLOGY CO LTD
- Filing Date
- 2026-03-17
- Publication Date
- 2026-06-05
AI Technical Summary
Existing sea cucumber powder preparation methods are difficult to effectively retain multiple active ingredients, resulting in low bioavailability, poor dissolution rate of active ingredients, and difficulty in controlling batch-to-batch quality stability, which cannot meet the needs of postoperative repair and cell regeneration.
A synergistic process of preliminary and deep extraction was adopted, using compound enzymes A and B to perform preliminary and deep enzymatic hydrolysis of the sea cucumber body wall, respectively. Combined with low-temperature vacuum concentration and spray drying, highly bioactive sea cucumber powder was prepared.
It significantly improved the dissolution rate of collagen peptides and sea cucumber polysaccharides, promoted postoperative repair and cell regeneration, and improved the bioavailability and quality stability of the product.
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Figure CN122139903A_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of food processing technology, and in particular relates to a sea cucumber powder that promotes postoperative repair and cell regeneration, its preparation method, and its application. Background Technology
[0002] Postoperative tissue repair and regeneration are crucial for surgical success and patient recovery. An ideal repair process requires efficient control of inflammation, promotion of granulation tissue formation, accelerated angiogenesis, and stimulation of parenchymal cell proliferation and differentiation. Currently, clinical practice often employs nutritional support, physical therapy, and certain growth factor drugs (such as recombinant human epidermal growth factor), but these methods still face challenges such as high cost, poor stability, unsatisfactory responses in some patients, or potential side effects. Therefore, developing postoperative rehabilitation aids that are naturally derived, have proven efficacy, high safety, and are cost-effective has significant clinical and social value.
[0003] Sea cucumbers, traditionally considered both food and medicine, are regarded as a nourishing delicacy. Modern research shows that the body wall of sea cucumbers is rich in various bioactive substances. Sea cucumber polysaccharides have activities such as immunomodulation, anticoagulation, and promotion of cell migration and proliferation; sea cucumber saponins have anti-inflammatory, antifungal, and certain immunomodulatory effects; collagen and polypeptides are important structural substances and signaling molecules for tissue repair and are easily absorbed; and various trace elements and vitamins provide essential nutrients for cell metabolism.
[0004] Although sea cucumbers and their crude extracts are widely believed in traditional medicine to aid wound healing and recovery, the preservation of active ingredients and the clarity of their efficacy depend heavily on specific processing methods. Traditional sea cucumber processing methods and subsequent simple pulverization often prioritize preservation and ease of consumption, easily leading to the degradation or inactivation of heat-sensitive and easily oxidized active ingredients such as polysaccharides, saponins, and active peptides with specific structures. Existing sea cucumber powder preparation methods are mostly single-process methods, such as direct hot air drying and pulverization or ordinary freeze-drying and pulverization. These methods either fail to maximize the retention of all active components or do not consider synergistic effects between different active components through process design. Furthermore, the lack of a complete, coordinated process chain—from raw material pretreatment and targeted extraction / protection of active ingredients to low-temperature drying and cell-level ultrafine pulverization—results in low bioavailability, poor dissolution rates of active ingredients, and difficulties in batch-to-batch quality stability control in the final product.
[0005] Therefore, in view of the shortcomings of the existing technology, there is an urgent need to provide a sea cucumber powder with a scientific preparation method and efficient retention of active ingredients, so as to enhance the added value of sea cucumber products. Summary of the Invention
[0006] To address the aforementioned technical problems, this invention proposes a sea cucumber powder that promotes postoperative repair and cell regeneration, along with its preparation method and application. By utilizing a combination of preliminary extraction and deep extraction, the dissolution rate of collagen peptides and sea cucumber polysaccharides is increased, resulting in the preparation of sea cucumber powder that effectively assists in promoting postoperative repair and cell regeneration.
[0007] To achieve the above objectives, this invention provides a method for preparing sea cucumber powder that promotes postoperative repair and cell regeneration, comprising the following steps: 1) The sea cucumber is rinsed with purified water, the viscera are removed, and it is air-dried until the moisture content is ≤5%, then pulverized to obtain a pretreated raw material; 2) The pretreated raw material obtained in step 1) is initially extracted with compound enzyme A to obtain a preliminary extraction mixture; 3) The preliminary extraction mixture obtained in step 2) is deeply extracted with compound enzyme B to inactivate the enzyme, thus obtaining an extraction mixture; 4) The extraction mixture obtained in step 3) is centrifuged to obtain the middle layer supernatant, and then concentrated under low temperature vacuum to 1 / 5 to 1 / 10 of the original volume to obtain a concentrate; 5) The concentrate obtained in step 4) is spray-dried and ultra-finely pulverized to obtain sea cucumber powder.
[0008] Preferably, the sea cucumber mentioned in step 1) is Liaoning sea cucumber, the air-drying temperature mentioned in step 1) is 20~25℃, and the particle size of the pulverized material mentioned in step 1) is 20~40 mesh.
[0009] Preferably, the compound enzyme A mentioned in step 2) is prepared by mixing cellulase and pectinase at a mass ratio of 1:1. The preliminary extraction mentioned in step 2) specifically involves mixing compound enzyme A, the pretreated raw material obtained in step 1) and purified water at a ratio of 0.1~0.3g:100g:1000mL, and enzymatically hydrolyzing for 1~2h in an environment with a pH of 4.5~5.5 and a temperature of 45~50℃.
[0010] More preferably, the cellulase has an activity of 50,000 to 60,000 U / g, and the pectinase has an activity of 40,000 to 60,000 U / g.
[0011] Preferably, the compound enzyme B mentioned in step 3) is prepared by mixing neutral protease and papain at a mass ratio of 1:1~3. The deep extraction mentioned in step 3) specifically involves mixing compound enzyme B with the preliminary extraction mixture obtained in step 2) at a ratio of 0.3~0.5g:100g, and enzymatically hydrolyzing it for 2~4h in an environment with a pH of 6.5~7.0 and a temperature of 55~60℃. The enzyme inactivation temperature mentioned in step 3) is 85~90℃, and the enzyme inactivation time is 15min.
[0012] More preferably, the neutral protease has an enzyme activity of 100,000 to 150,000 U / g, and the papain has an enzyme activity of 800,000 to 1,200,000 U / g.
[0013] Preferably, in step 4), the centrifugation temperature is 4~10℃, the centrifugation speed is 6000~8000rpm, the centrifugation time is 10~20min, the low-temperature vacuum concentration speed is 50~100rpm, the low-temperature vacuum concentration temperature is 50~55℃, the low-temperature vacuum concentration vacuum degree is -0.08~-0.095MPa, and the low-temperature vacuum concentration is carried out in a water bath.
[0014] Preferably, in step 5), the spray drying rotation speed is 15000~25000 rpm, the spray drying feed rate is 20~50 mL / min, the spray drying inlet air temperature is 150~180℃, the spray drying outlet air temperature is 80~90℃, and the spray drying time is 10~30 s.
[0015] The present invention also provides sea cucumber powder prepared by the aforementioned preparation method.
[0016] The present invention also provides the application of the sea cucumber powder in the preparation of products that help promote postoperative repair and cell regeneration.
[0017] Compared with the prior art, the present invention has the following advantages and technical effects: This invention provides a sea cucumber powder that promotes postoperative repair and cell regeneration, along with its preparation method and applications. Utilizing a combination of preliminary and deep extraction, the dissolution rate of collagen peptides and sea cucumber polysaccharides is improved, resulting in a sea cucumber powder that effectively aids in promoting postoperative repair and cell regeneration. Liaoning sea cucumber is selected due to its high content of collagen and polysaccharides. Cellulase and pectinase are used to degrade the cellulose and pectin network of the sea cucumber's body wall, fully exposing the internal components. Neutral protease and papain are used to hydrolyze large molecules such as collagen into smaller peptides and amino acids. Since the degradation products of sea cucumber polysaccharides and collagen are readily soluble in water, the amount of water added is strictly controlled to minimize water solubility loss. The preparation process is strictly controlled at low temperatures. Through enzymatic hydrolysis and low-temperature treatment, large protein and sugar molecules are partially degraded, which not only helps release the effective components but also significantly improves the absorption rate by the human body. The entire process is carried out at low temperatures to protect the structure of polysaccharides and collagen from damage, resulting in a sea cucumber powder with higher bioactivity. Attached Figure Description
[0018] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0019] Figure 1The results of tensile strength measurements of wounds in each group of mice; Figure 2 The results of serum IL-8 level measurement in each group of mice; Figure 3 The results of serum IL-10 level measurement in mice of each group; Figure 4 The results show the serum NO levels measured in each group of mice. Detailed Implementation
[0020] Various exemplary embodiments of the present invention are now described in detail. This detailed description should not be considered as a limitation of the invention, but rather as a more detailed description of certain aspects, features, and embodiments of the invention. Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of the invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with those documents. In the event of any conflict with any incorporated document, the contents of this specification shall prevail.
[0021] Example 1 1) Liaoning sea cucumbers are rinsed with purified water to remove sand and soil, and their internal organs are removed. They are then air-dried at 20℃ until the moisture content is ≤5%, and pulverized to a particle size of 30 mesh to obtain pretreated raw materials. 2) The pretreated raw materials are initially extracted using compound enzyme A (a mixture of cellulase with an activity of 60,000 U / g and pectinase with an activity of 50,000 U / g at a mass ratio of 1:1). Specifically, compound enzyme A, the pretreated raw materials, and purified water are mixed at a ratio of 0.2g:100g:1000mL and enzymatically hydrolyzed at pH 5 and 48℃ for 1.5 hours to obtain a preliminary extraction mixture. 3) The preliminary extraction mixture is then further extracted using compound enzyme B (a mixture of neutral protease with an activity of 120,000 U / g and papain with an activity of 1,000,000 U / g at a mass ratio of 1:2). The mixture consists of: 1) Compound enzyme B and the preliminary extraction mixture are mixed at a ratio of 0.4g:100g, enzymatically hydrolyzed for 3 hours at pH 6.8 and temperature 58℃, and then inactivated at 85℃ for 15 minutes to obtain the extraction mixture; 2) The extraction mixture is centrifuged at 7000rpm for 15 minutes at 4℃, and the middle layer is collected. The mixture is then concentrated to 1 / 8 of its original volume under low-temperature vacuum heating in a water bath at a speed of 80rpm, a temperature of 50℃, and a vacuum degree of -0.095MPa to obtain the concentrate; 3) The concentrate is spray-dried for 10-30 seconds at a speed of 20000rpm, a feed rate of 35mL / min, an inlet air temperature of 150~180℃, and an outlet air temperature of 80~90℃, and then ultra-finely pulverized to obtain sea cucumber powder.
[0022] Example 2 1) Liaoning sea cucumber is rinsed with purified water to remove sand and soil, and its internal organs are removed. It is then air-dried at 20℃ until the moisture content is ≤5%, and pulverized to a particle size of 20 mesh to obtain pretreated raw material; 2) The pretreated raw material is initially extracted with compound enzyme A (a mixture of cellulase with an enzyme activity of 50,000 U / g and pectinase with an enzyme activity of 40,000 U / g at a mass ratio of 1:1). Specifically, compound enzyme A, pretreated raw material, and purified water are mixed at a ratio of 0.1g:100g:1000mL, and enzymatically hydrolyzed for 1 hour at a pH of 4.5 and a temperature of 45℃ to obtain a preliminary extraction mixture; 3) The preliminary extraction mixture is further extracted with compound enzyme B (a mixture of neutral protease with an enzyme activity of 100,000 U / g and papain with an enzyme activity of 800,000 U / g at a mass ratio of 1:1). The mixture consists of: 1) Compound enzyme B and the preliminary extraction mixture are mixed at a ratio of 0.3g:100g, enzymatically hydrolyzed for 2 hours at pH 6.5 and temperature 55℃, and then inactivated at 85℃ for 15 minutes to obtain the extraction mixture; 2) The extraction mixture is centrifuged at 4℃ and 6000rpm for 20 minutes, and the middle layer is collected. The mixture is then concentrated to 1 / 5 of its original volume under low-temperature vacuum heating in a water bath at a speed of 50rpm, a temperature of 50℃, and a vacuum degree of -0.08MPa to obtain the concentrate; 3) The concentrate is spray-dried for 10-30 seconds at a speed of 15000rpm, a feed rate of 20mL / min, an inlet air temperature of 150~180℃, and an outlet air temperature of 80~90℃, and then ultra-finely pulverized to obtain sea cucumber powder.
[0023] Example 3 1) Liaoning sea cucumbers are rinsed with purified water to remove sand and soil, and their internal organs are removed. They are then air-dried at 25℃ until the moisture content is ≤5%, and pulverized to a particle size of 40 mesh to obtain pretreated raw materials. 2) The pretreated raw materials are initially extracted using compound enzyme A (a mixture of cellulase and pectinase with an activity of 60,000 U / g at a mass ratio of 1:1). Specifically, compound enzyme A, the pretreated raw materials, and purified water are mixed at a ratio of 0.3g:100g:1000mL and enzymatically hydrolyzed for 2 hours at pH 5.5 and a temperature of 50℃ to obtain a preliminary extraction mixture. 3) The preliminary extraction mixture is then further extracted using compound enzyme B (a mixture of neutral protease with an activity of 150,000 U / g and papain with an activity of 1,200,000 U / g at a mass ratio of 1:3). The following steps were performed: 1) Mix compound enzyme B with the preliminary extraction mixture at a ratio of 0.5g:100g, and enzymatically hydrolyze the mixture for 4 hours at pH 7.0 and 60℃, then inactivate the enzyme at 90℃ for 15 minutes to obtain the extraction mixture; 2) Centrifuge the extraction mixture at 10℃ and 8000rpm for 10 minutes, take the middle layer supernatant, and concentrate it to 1 / 10 of the original volume in a water bath under low-temperature vacuum heating at 100rpm, 55℃, and -0.095MPa to obtain the concentrate; 3) Spray dry the concentrate at 25000rpm, 50mL / min, 150~180℃ inlet air temperature, and 80~90℃ outlet air temperature for 10~30s, then pulverize it to obtain sea cucumber powder.
[0024] Comparative Example 1 1) Liaoning sea cucumber is washed with pure water to remove sand and soil, and the internal organs are removed. It is then air-dried at 20℃ until the moisture content is ≤5%, and the particle size is 30 mesh to obtain pre-treated raw material. 2) One-step enzymatic hydrolysis: The pretreated raw material is extracted with compound enzyme C (cellulase with an enzyme activity of 60,000 U / g, pectinase with an enzyme activity of 50,000 U / g, neutral protease with an enzyme activity of 120,000 U / g, and papain with an enzyme activity of 1,000,000 U / g mixed in a mass ratio of 1:1:1:2). Specifically, compound enzyme C, pretreated raw material, and purified water are mixed at a ratio of 0.6g:100g:1000mL, and enzymatic hydrolysis is carried out in an environment with pH 5 and temperature of 48℃ for 1.5h. The enzyme is then inactivated at 85℃ for 15min to obtain the extract mixture. 3) Extract the mixture, centrifuge at 7000 rpm for 15 min at 4℃, take the middle layer clear liquid, and concentrate it to 1 / 8 of the original volume in a water bath under low temperature vacuum at 80 rpm, 50℃ and -0.095 MPa to obtain the concentrate. 4) The concentrate is spray-dried for 10-30 seconds at a speed of 20,000 rpm, a feed rate of 35 mL / min, an inlet air temperature of 150-180℃, and an outlet air temperature of 80-90℃, and then ultra-finely pulverized to obtain sea cucumber powder.
[0025] Comparative Example 2 1) Liaoning sea cucumber is washed with pure water to remove sand and soil, and the internal organs are removed. It is then air-dried at 20℃ until the moisture content is ≤5%, and the particle size is 30 mesh to obtain pre-treated raw material. 2) Pre-treatment of raw materials: Preliminary extraction of compound enzyme A (a mixture of cellulase with an enzyme activity of 60,000 U / g and pectinase with an enzyme activity of 50,000 U / g at a mass ratio of 1:1) is carried out. Specifically, compound enzyme A, pre-treated raw materials and purified water are mixed at a ratio of 0.2g:100g:1000mL and enzymatically hydrolyzed for 1.5h in an environment with a pH of 5 and a temperature of 48℃ to obtain a preliminary extraction mixture. 3) Preliminary extraction of the mixture: Papain with an enzyme activity of 1.2 million U / g was used for deep extraction. Specifically, the papain with an enzyme activity of 1.2 million U / g was mixed with the preliminary extraction mixture at a ratio of 0.4g:100g, and enzymatically hydrolyzed for 3 hours at a pH of 6.5 and a temperature of 60℃. The enzyme was then inactivated at 85℃ for 15 minutes to obtain the extraction mixture. 4) Extract the mixture, centrifuge at 7000 rpm for 15 min at 4℃, take the middle layer clear liquid, and concentrate it to 1 / 8 of the original volume in a water bath under low temperature vacuum heating at 80 rpm, 50℃ and -0.095 MPa to obtain the concentrate. 5) The concentrate is spray-dried for 10-30 seconds at a speed of 20,000 rpm, a feed rate of 35 mL / min, an inlet air temperature of 150-180℃, and an outlet air temperature of 80-90℃, and then ultra-finely pulverized to obtain sea cucumber powder.
[0026] Experimental Example 1 Sixty 10-week-old SPF-grade male db / db type diabetic mice and twelve healthy db / m mice of the same age and sex were housed in an environment with an ambient temperature of 22±1℃ and a relative humidity of 50%~60%, with free access to food and water and acclimatization. Then, an animal trauma model was established. The diabetic mice were anesthetized with 4% chloral hydrate, and their backs were shaved and disinfected. A 1.5cm longitudinal incision was made on each side of the mouse's back, cutting through the muscle and reaching the abdominal cavity, to simulate the layered suturing of muscle and skin after surgery. After completion, the area was disinfected with 2% povidone-iodine, and penicillin G sodium (1000 IU / g·BW) was injected to prevent infection. The mice were fasted for 6 hours post-surgery, and the day of surgery was recorded as day 0. The twelve healthy db / m mice served as a normal control group, receiving the same volume of distilled water by gavage. The animal trauma model mice were divided into 5 groups of 12 mice each. The control group mice were given the same volume of distilled water by gavage. The whey protein group mice were given whey protein by gavage at a dose of 1 g / kg (body weight). The experimental group mice were given sea cucumber powder prepared in Example 1 by gavage at a dose of 1 g / kg (body weight). The experimental group mice were given sea cucumber powder prepared in Comparative Example 1 by gavage at a dose of 1 g / kg (body weight). The experimental group mice were given sea cucumber powder prepared in Comparative Example 2 by gavage at a dose of 1 g / kg (body weight). The whey protein and sea cucumber powder were prepared to a concentration of 0.1 mL / 10 g.
[0027] I. Determination of wound swelling resistance: Mice from each group were sacrificed on day 14. A rectangular piece of skin was harvested at sacrifice, 0.5 cm wide at the midline and 0.5 cm from each side of the wound. Subcutaneous fat tissue was removed. Both ends of the skin were connected to the ends of a tension sensor. The skin was slowly stretched by adjusting a knob, and the tension at the point of skin breakage was recorded using a multifunctional biosignal acquisition system. The skin thickness was measured, and the cross-sectional area was calculated. The tensile strength of the skin was calculated by dividing the tension by the cross-sectional area.
[0028] II. Biochemical index determination: Commercially available mouse IL-8, IL-10 (ELISA) assay kits and mouse NO assay kits were used. After euthanizing the mice, blood was collected from the eyeballs, centrifuged, and serum was obtained to measure the levels of IL-8, IL-10, and NO in the serum.
[0029] III. Data Processing: One-way ANOVA was performed using SPSS 20.0 statistical software.
[0030] IV. Results: 1. Tensile strength of the wound: Table 1. Tensile strength of wounds in mice of each group on day 14
[0031] Note:" "Compared with the model control group, P < 0.05," "Compared with the model control group, P < 0.01."
[0032] As shown in Table 1 and Figure 1 As shown, the wound tensile strength test revealed that the wound tensile strength of Experiment 1 group was significantly higher than that of the model control group, the wound tensile strength of Experiment 2 group was higher than that of the model control group, and the wound tensile strength of Experiment 3 group showed no significant change compared with the model control group. This indicates that the wound healing degree of the mice in Experiment 1 group corresponding to the sea cucumber powder prepared in Example 1 was significantly better than that of the model control group.
[0033] 2. Serum IL-8 and IL-10 levels in mice: Table 2 Serum IL-8 levels in mice of each group
[0034] Note:" "Compared with the model control group, P < 0.05," "Compared with the model control group, P < 0.01."
[0035] Table 3 Serum IL-10 levels in mice of each group
[0036] Note:" "Compared with the model control group, P < 0.05," "Compared with the model control group, P < 0.01."
[0037] As shown in Table 2 and Figure 2 As shown, the serum IL-8 level test revealed that the serum IL-8 levels in Experiment 1, Experiment 2 and Experiment 3 were significantly lower than those in the model control group. The increase in the inflammatory factor IL-8 may prolong the inflammatory period and cause impaired wound healing in diabetic patients. This indicates that the inflammatory period of mice corresponding to the sea cucumber powder prepared in Example 1 and Comparative Examples 1 and 2 was significantly shorter than that in the model control group.
[0038] As shown in Table 3 and Figure 3 As shown, the serum IL-10 level test revealed that the serum IL-10 levels in Experiment 1 and Experiment 2 were significantly higher than those in the model control group. The anti-inflammatory factor IL-10 regulates fibroblast regeneration and promotes tissue repair, indicating that the tissue repair ability of mice corresponding to the sea cucumber powder prepared in Example 1 and Comparative Example 1 is significantly stronger than that of the model control group.
[0039] 3. Serum NO levels in mice: Table 4 Serum NO levels in mice of each group
[0040] Note:" "Compared with the model control group, P < 0.05," "Compared with the model control group, P < 0.01."
[0041] As shown in Table 4 and Figure 4 As shown, the serum NO level test revealed that the serum NO level in Experiment 1 group was significantly higher than that in the model control group. NO is the most effective factor in promoting VEGF expression, indicating that the sea cucumber powder prepared in Example 1 can promote the increase of NO level in diabetic mice, thereby promoting the formation of new blood vessels and improving the healing of chronic wounds.
[0042] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.
Claims
1. A method for preparing sea cucumber powder that promotes postoperative repair and cell regeneration, characterized in that, Includes the following steps: 1) The sea cucumbers are rinsed with purified water, the internal organs are removed, and they are air-dried until the moisture content is ≤5%. They are then crushed to obtain pre-treated raw materials. 2) The pretreated raw material obtained in step 1) is initially extracted by compound enzyme A to obtain a preliminary extraction mixture; 3) The preliminary extraction mixture obtained in step 2) is subjected to deep extraction with compound enzyme B to inactivate the enzyme and obtain the extraction mixture; 4) The extracted mixture obtained in step 3) is centrifuged to obtain the middle layer supernatant, and then concentrated under low temperature and vacuum to 1 / 5 to 1 / 10 of the original volume to obtain the concentrate; 5) The concentrate obtained in step 4) is spray-dried and ultra-finely pulverized to obtain sea cucumber powder.
2. The preparation method according to claim 1, characterized in that, The sea cucumber mentioned in step 1) is Liaoning sea cucumber, the air-drying temperature mentioned in step 1) is 20~25℃, and the particle size of the pulverized material mentioned in step 1) is 20~40 mesh.
3. The preparation method according to claim 1, characterized in that, The compound enzyme A mentioned in step 2) is prepared by mixing cellulase and pectinase at a mass ratio of 1:
1. The preliminary extraction mentioned in step 2) specifically involves mixing compound enzyme A, the pretreated raw material obtained in step 1) and purified water at a ratio of 0.1~0.3g:100g:1000mL, and enzymatically hydrolyzing for 1~2h in an environment with a pH of 4.5~5.5 and a temperature of 45~50℃.
4. The preparation method according to claim 3, characterized in that, The cellulase has an activity of 50,000 to 60,000 U / g, and the pectinase has an activity of 40,000 to 60,000 U / g.
5. The preparation method according to claim 1, characterized in that, The compound enzyme B mentioned in step 3) is prepared by mixing neutral protease and papain at a mass ratio of 1:1~3. The deep extraction mentioned in step 3) specifically involves mixing compound enzyme B with the preliminary extraction mixture obtained in step 2) at a ratio of 0.3~0.5g:100g, and enzymatically hydrolyzing it for 2~4h in an environment with a pH of 6.5~7.0 and a temperature of 55~60℃. The enzyme inactivation temperature mentioned in step 3) is 85~90℃, and the enzyme inactivation time is 15min.
6. The preparation method according to claim 5, characterized in that, The neutral protease has an enzyme activity of 100,000 to 150,000 U / g, and the papain has an enzyme activity of 800,000 to 1,200,000 U / g.
7. The preparation method according to claim 1, characterized in that, In step 4), the centrifugation temperature is 4~10℃, the centrifugation speed is 6000~8000rpm, and the centrifugation time is 10~20min. In step 4), the low-temperature vacuum concentration speed is 50~100rpm, the low-temperature vacuum concentration temperature is 50~55℃, and the low-temperature vacuum concentration vacuum degree is -0.08~-0.095MPa. The low-temperature vacuum concentration is carried out in a water bath.
8. The preparation method according to claim 1, characterized in that, In step 5), the spray drying speed is 15000~25000 rpm, the spray drying feed rate is 20~50 mL / min, the spray drying inlet air temperature is 150~180℃, the spray drying outlet air temperature is 80~90℃, and the spray drying time is 10~30 s.
9. Sea cucumber powder prepared by the preparation method according to any one of claims 1 to 8.
10. The use of sea cucumber powder as described in claim 9 in the preparation of products that assist in promoting postoperative repair and cell regeneration.