Moisturizing soothing skin care product containing camellia oil and preparation method thereof

Abstract

The application discloses a moisturizing and soothing skincare product containing camellia oil and a preparation method thereof, and relates to the technical field of skincare products, and contains the following components in percentage by mass: 5.0%-15.0% of camellia oil; 1.0%-8.0% of a chelate of folium phyllostachy sitchensis flavone and spilanthes acmella-alkaloid; 3.0%-12.0% of a humectant; 1.0%-5.0% of an emulsifier; 0.1%-1.5% of a thickening agent; 0.1%-1.0% of a preservative; 0.01%-0.1% of a chelating agent; 0.01%-2.0% of a pH regulator; and the rest is deionized water; the folium phyllostachy sitchensis flavone is combined with the spilanthes acmella-alkaloid into a stable chelate through low-temperature molecular chelation technology, and then the chelate is compounded with high-purity cold-pressed camellia oil in proportion, so that the defects of natural plant extracts, such as easy oxidation, easy inactivation and poor transdermal property, are solved, and the synergistic effect of four functions of moisturizing, soothing, anti-oxidation and stability maintenance is realized.

Description

Technical Field

[0001] This invention relates to the field of skincare technology, specifically to a moisturizing and soothing skincare product containing camellia oil and its preparation method. Background Technology

[0002] With the accelerated pace of modern life, the increasing severity of environmental pollution, and the widespread adoption of cosmetic and phototherapy treatments, the number of people with sensitive skin is showing a year-on-year upward trend. During seasonal changes, when exposed to environmental stimuli, when performing improper skincare procedures, or after undergoing phototherapy treatments such as laser or IPL skin rejuvenation, the skin often experiences a series of uncomfortable symptoms such as redness, stinging, dryness, peeling, burning, and itching. From a pathological perspective, the core issue behind these symptoms lies in the damage to the skin barrier function, leading to increased transepidermal water loss, excessive release of inflammatory mediators, and the continuous accumulation of free radicals in the skin. Therefore, developing skincare products with moisturizing, soothing, antioxidant, and barrier-repairing effects is of great practical significance in meeting the skincare needs of people with sensitive skin and improving their skin health.

[0003] Most commercially available soothing and repairing skincare products currently on the market use a crude method of adding single plant extracts, which has several common technical defects. First, natural plant active ingredients, such as flavonoids and alkaloids, have poor stability and are prone to oxidation and degradation during formulation and storage, leading to a decrease in activity and thus affecting the efficacy of the skincare products. For example, some skincare products containing plant extracts have a significantly reduced content of effective ingredients after being stored for a period of time, failing to achieve the expected skincare effects. Second, some active molecules lack sufficient transdermal permeability, making it difficult to effectively penetrate the stratum corneum and reach the basal layer of the epidermis and even the dermis to exert their due effects, thus greatly reducing the actual effect of the skincare products. Third, most existing technologies simply involve the physical mixing of multiple plant extracts, lacking synergistic integration at the molecular level between the ingredients, making it difficult to maximize efficacy and limiting the potential for efficacy enhancement. Summary of the Invention

[0004] The purpose of this invention is to overcome the shortcomings of the prior art and provide a moisturizing and soothing skin care product containing camellia oil and its preparation method. By using low-temperature molecular chelation technology, the flavonoids of bamboo leaf and alkaloids of purslane are combined into a stable chelate, which is then compounded with high-purity cold-pressed camellia oil in a certain proportion. This solves the defects of natural plant extracts such as easy oxidation, easy inactivation, and poor transdermal permeability, and achieves a synergistic effect of moisturizing, soothing, anti-oxidation, and stabilizing.

[0005] To solve the above-mentioned technical problems, the present invention provides the following technical solution: a moisturizing and soothing skin care product containing camellia oil, comprising the following components by weight percentage: Camellia oil 5.0%-15.0%; Lophatherum gracile flavonoids-purslane alkaloid chelate 1.0%-8.0%; Moisturizer content: 3.0%-12.0%; Emulsifier 1.0%-5.0%; Thickener 0.1%-1.5%; Preservatives: 0.1%-1.0%; Chelating agent 0.01%-0.1%; pH adjuster 0.01%-2.0% The remainder is deionized water; The camellia oil mentioned is high-purity cold-pressed camellia oil, with an oleic acid content of not less than 75%, a linoleic acid content of not less than 8%, and a vitamin E content of not less than 80 mg / kg; The chelate of flavonoids from Lophatherum gracile and alkaloids from Portulaca oleracea was prepared by the following method: total flavonoid extract from Lophatherum gracile and alkaloid extract from Portulaca oleracea were mixed at a mass ratio of 1:0.5-2.0, and stirred for 1-6 hours at a temperature of 4-25℃ and a pH of 6.0-7.5. After purification by dialysis or ultrafiltration, the chelate was freeze-dried.

[0006] Furthermore, the total flavonoid content in the total flavonoid extract of Lophatherum gracile is not less than 50%, and the total alkaloid content in the purslane alkaloid extract is not less than 20%.

[0007] Furthermore, the moisturizer is selected from one or more of glycerin, butylene glycol, propylene glycol, sodium hyaluronate, trehalose, betaine, and panthenol.

[0008] Furthermore, the emulsifier is selected from one or more of cetearyl oleate, sorbitan oleate, polyglycerol-3-methyl glucoside distearate, cetearyl glucoside, and PEG-40 hydrogenated castor oil.

[0009] Furthermore, the thickener is selected from one or more of carbomer, xanthan gum, acrylic / C10-30 alkanol acrylate crosspolymers, and hydroxyethyl cellulose.

[0010] Furthermore, the preservative is selected from one or more of phenoxyethanol, ethylhexylglycerin, p-hydroxyacetophenone, and octyl glycol; the chelating agent is disodium EDTA.

[0011] Furthermore, the pH adjuster is selected from one or more of arginine, triethanolamine, sodium hydroxide, and citric acid, and is used to adjust the pH value of the skin care product to 5.5-6.5.

[0012] Furthermore, the preparation method of the total flavonoid extract of Lophatherum gracile is as follows: Take dried bamboo leaves as raw material, pulverize them with a pulverizer and pass them through a 40-60 mesh sieve to obtain bamboo leaf powder. Add 60%-80% (v / v) ethanol aqueous solution to the bamboo leaf powder at a material-to-liquid ratio of 1:10-20. Perform ultrasonic-assisted extraction at a temperature of 40-50℃, with an ultrasonic power of 200-400W and an extraction time of 30-60 minutes. After extraction, filter the solution with filter cloth or filter paper and collect the filtrate. Concentrate the filtrate under reduced pressure at 40-50℃ and a vacuum degree of -0.08 to -0.095 MPa to recover ethanol until there is no alcohol odor, thus obtaining bamboo leaf concentrate.

[0013] The concentrated extract of Lophatherum gracile was loaded onto a pretreated macroporous adsorption resin column at a flow rate of 1-3 BV / h. After loading, 2-3 column volumes of deionized water were used to elute the column at a flow rate of 2-4 BV / h to remove water-soluble impurities such as sugars and proteins. A 50%-70% (v / v) ethanol aqueous solution was used as the eluent at a flow rate of 1-3 BV / h. The flavonoid eluent was collected and concentrated under reduced pressure at 40-50℃ and a vacuum of -0.08 to -0.095 MPa until alcohol-free, yielding a concentrated flavonoid extract. This concentrated extract was freeze-dried at -40 to -50℃ and a vacuum of 10-30 Pa for 24-48 hours to obtain the total flavonoid extract of Lophatherum gracile. The total flavonoid content in the extract was determined using the sodium nitrite-aluminum nitrate colorimetric method with rutin as a standard, and should not be less than 50%.

[0014] Furthermore, the preparation method of the purslane alkaloid extract is as follows: Take the dried aerial parts of purslane, pulverize them with a pulverizer and pass them through a 40-60 mesh sieve to obtain purslane powder. Add an acidic ethanol solution to the purslane powder at a material-to-liquid ratio of 1:8-15. The acidic ethanol solution is prepared by adding hydrochloric acid or sulfuric acid to an ethanol aqueous solution with a volume fraction of 60%-80% to adjust the pH value to 2-3. Perform ultrasonic-assisted extraction at a temperature of 35-45℃, with an ultrasonic power of 200-400W and an extraction time of 40-80 minutes. After extraction, filter the solution with filter cloth or filter paper, collect the filtrate, and concentrate the filtrate under reduced pressure at 40-50℃ and a vacuum degree of -0.08 to -0.095MPa to recover the ethanol and obtain a concentrated purslane solution.

[0015] Slowly add 10% ammonia or sodium hydroxide solution to the purslane concentrate while stirring, adjusting the pH to 9-10. Then, transfer the alkalized concentrate to a separatory funnel, add an equal volume of chloroform or ethyl acetate, and extract by shaking for 5-10 minutes. Allow the layers to separate, collecting the lower organic phase (for chloroform extraction) or the upper organic phase (for ethyl acetate extraction). Repeat the extraction process 2-3 times, combining all organic phases. Concentrate the combined organic phase to dryness under reduced pressure at 30-40℃ and a vacuum of -0.08 to -0.095 MPa to obtain crude alkaloids. Freeze-dry the crude product at -40 to -50℃ and a vacuum of 10-30 Pa for 12-24 hours to obtain the purslane alkaloid extract. The total alkaloid content in the extract should be not less than 20% when determined by the bromocresol green acid dye colorimetric method.

[0016] On the other hand, a method for preparing a moisturizing and soothing skincare product containing camellia oil, the method comprising the following steps: S1: Weigh the required amounts of moisturizer, thickener, chelating agent (disodium EDTA), and deionized water according to the mass percentage of each component in the skin care product formula. First, add the moisturizer, thickener, and chelating agent to a portion of the deionized water in sequence while stirring to fully wet and disperse the solid powder. Then, heat the mixture to 70-80℃ and stir continuously at a rate of 200-500 rpm for 15-30 minutes under the heat preservation condition until all components are completely dissolved and a homogeneous and transparent aqueous phase is formed. Keep warm for later use.

[0017] S2: Weigh out the high-purity cold-pressed camellia oil and emulsifier according to the formula. Add the camellia oil and emulsifier together into the oil phase container, mix and heat to 70-80℃. Stir at 100-300 rpm for 10-20 minutes under the heat preservation condition until the emulsifier is completely dissolved and forms a uniform oil phase with the camellia oil. Keep warm for later use.

[0018] S3: While maintaining both the aqueous and oil phases at 75°C, slowly and uniformly pour the oil phase into the aqueous phase, and simultaneously turn on the homogenizer for homogenization and emulsification. Set the homogenization speed to 3000-6000 rpm, preferably 4000-5000 rpm, and homogenize for 3-8 minutes. During homogenization, the homogenizer head should always be submerged below the liquid surface to avoid entraining too much air. After homogenization, a uniform and delicate primary emulsion is obtained.

[0019] S4: Cool the above colostrum naturally or with circulating water to 40-45℃ under stirring. At this temperature, add the prescribed amount of bamboo leaf flavonoid-purslane alkaloid chelate and preservative. To improve the dispersion uniformity of the chelate, the chelate can be ground or stirred into a uniform slurry with a small amount of glycerol or deionized water (taken from the total amount of the formula) beforehand, and then added to the colostrum. After adding, continue stirring at a rate of 200-400 rpm for 10-20 minutes to ensure that the chelate and preservative are fully mixed and uniform.

[0020] S5: Slowly add the pH adjuster dropwise to the mixture while stirring. Monitor the pH value of the system with a pH meter in real time until the pH value reaches 5.5-6.5. Add the remaining deionized water to the full amount of the formula, continue stirring for 10-15 minutes, and finally stop stirring. Cool the material to room temperature to obtain the moisturizing and soothing skin care product containing camellia oil.

[0021] Compared with existing technologies, this moisturizing and soothing skincare product containing camellia oil and its preparation method have the following beneficial effects: This invention employs low-temperature molecular chelation technology to combine bamboo leaf flavonoids and purslane alkaloids into a stable chelate, effectively solving the problem of poor stability of natural plant active ingredients. The chelate structure improves the hydrophilic-lipophilic balance of the active ingredients, enhancing transdermal absorption. High-purity cold-pressed camellia oil and the chelate are combined in the optimal ratio to produce a synergistic effect. Camellia oil forms a biomimetic protective film to lock in moisture and repair the skin barrier, while the chelate effectively scavenge free radicals, inhibit histamine release, and downregulate the expression of inflammatory factors. Together, they increase the moisture content of the stratum corneum, reduce transepidermal water loss, inhibit the release of inflammatory factors, and increase the skin barrier index.

[0022] Other advantages, objectives and features of the invention will be set forth in part in the description which follows, and in part will be apparent to those skilled in the art from the following examination or study, or may be learned from the practice of the invention. Attached Figure Description

[0023] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are merely some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without any creative effort.

[0024] Figure 1 A flowchart illustrating the preparation method of a moisturizing and soothing skincare product containing camellia oil, namely, a chelate of bamboo leaf flavonoids and purslane alkaloids; Figure 2This is a flowchart illustrating a method for preparing a moisturizing and soothing skincare product containing camellia oil. Detailed Implementation

[0025] To further illustrate the technical means and effects of the present invention in achieving its intended purpose, the following detailed description of the specific implementation methods, structures, features, and effects of the present invention, in conjunction with the accompanying drawings and preferred embodiments, is provided below.

[0026] Example 1 like Figure 1 As shown, 500g of dried bamboo leaves were pulverized using a pulverizer and passed through a 60-mesh sieve to obtain bamboo leaf powder. The powder was added to 5000mL of 70% ethanol aqueous solution and ultrasonically extracted for 45 minutes in a water bath at 45℃ with an ultrasonic power of 300W. The extract was filtered through a filter cloth, and the filtrate was concentrated under reduced pressure at 45℃ and a vacuum of -0.09MPa. Ethanol was recovered until no alcohol odor was detected, yielding approximately 600mL of concentrated bamboo leaf extract.

[0027] The concentrated solution was loaded onto a pretreated D101 macroporous adsorption resin column (column inner diameter 5 cm, resin packing height 50 cm) at a flow rate of 2 BV / h. After loading, the column was eluted with deionized water at a flow rate of 3 BV / h for 2.5 column volumes, and then eluted with 60% ethanol aqueous solution at a flow rate of 2 BV / h. The eluent was collected.

[0028] The eluent was concentrated under reduced pressure at 45℃ and -0.09MPa until alcohol-free to obtain a flavonoid concentrate. The concentrate was freeze-dried at -45℃ and 20Pa for 36 hours to obtain 48.5g of total flavonoid extract from Lophatherum gracile. Using rutin as a standard, the total flavonoid content was determined to be 56.3% by the sodium nitrite-aluminum nitrate colorimetric method.

[0029] Take 500g of dried purslane aerial parts, pulverize them with a pulverizer and pass them through a 60-mesh sieve to obtain purslane powder. Add the powder to 4000mL of 70% (v / v) acidic ethanol solution. The pH of the acidic ethanol solution was adjusted to 2.5 with hydrochloric acid beforehand. The solution was ultrasonically extracted for 60 minutes in a 40℃ water bath with an ultrasonic power of 300W. The extract was filtered through a filter cloth, and the filtrate was concentrated under reduced pressure at 45℃ and a vacuum of -0.09MPa. The ethanol was recovered to obtain approximately 450mL of concentrated purslane extract.

[0030] Ammonia solution with a mass fraction of 10% was slowly added dropwise to the concentrate under stirring to adjust the pH value to 9.5. The alkalized concentrate was transferred to a separatory funnel, and an equal volume of chloroform was added. The mixture was shaken and extracted for 8 minutes, allowed to stand for separation, and the lower chloroform layer was collected. The extraction was repeated 3 times, and the chloroform layers were combined. The combined chloroform solution was concentrated to dryness under reduced pressure at 35°C and a vacuum of -0.09 MPa to obtain crude alkaloids. The crude product was freeze-dried at -45°C and a vacuum of 20 Pa for 18 hours to obtain 15.3 g of Portulaca oleracea alkaloid extract. The total alkaloid content was determined to be 24.7% by the bromocresol green acid dye colorimetric method.

[0031] Accurately weigh 5.0 g of the prepared total flavonoid extract from *Lophatherum gracile* and 5.0 g of the alkaloid extract from *Portulaca oleracea*, i.e., a mass ratio of 1:1. Add both to 500 mL of phosphate buffer solution with a pH of 7.2 and stir until completely dissolved. At this point, the total solute mass-volume concentration is 2.0%. Place the reaction vessel in a 10℃ constant temperature device and stir continuously at a rate of 200 rpm for 4 hours. After the reaction, transfer the entire reaction solution to a dialysis bag with a molecular weight cutoff of 3500 Da, seal the bag tightly, and place it in a container filled with deionized water. Dialyze at 6℃ for 18 hours, changing the deionized water every 6 hours. After dialysis, collect the retentate in the dialysis bag and freeze-dry it at -45℃ and a vacuum of 20 Pa for 30 hours to obtain 9.2 g of *Lophatherum gracile* flavonoid-Portulaca oleracea alkaloid chelate, with a yield of 92.0%.

[0032] Infrared spectroscopy analysis revealed a significant red shift and broadening of the hydroxyl stretching vibration peak near 3400 cm⁻¹ in the chelate, indicating the formation of hydrogen bonds between the flavonoid hydroxyl group and the nitrogen atom of the alkaloid. Differential scanning calorimetry analysis showed the appearance of a single new melting point peak in the chelate, proving the formation of a homogeneous intermolecular chelate.

[0033] Example 2 like Figure 2 As shown, the components were weighed according to the following mass percentages: 8.0% high-purity cold-pressed camellia oil; 3.0% bamboo leaf flavonoid-purslane alkaloid chelate; 5.0% glycerol; 3.0% butylene glycol; 0.1% sodium hyaluronate; 2.0% cetearyl oleate; 1.0% sorbitan oleate; 0.2% carbomer; 0.1% xanthan gum; 0.05% disodium EDTA; 0.6% phenoxyethanol / ethylhexylglycerin compound preservative; 0.12% arginine (used to adjust pH to 6.0); deionized water to 100%.

[0034] Add the prescribed amounts of glycerin, butylene glycol, sodium hyaluronate, carbomer, xanthan gum, and disodium EDTA to deionized water in sequence. After stirring and dispersing, heat to 75°C and stir at 300 rpm for 20 minutes until all components are completely dissolved, forming a homogeneous and transparent aqueous phase. Keep warm at 75°C for later use.

[0035] Mix the high-purity cold-pressed camellia oil, cetearyl oleate, and sorbitan oleate in the specified amounts, heat to 75°C, and stir at 200 rpm for 15 minutes until the emulsifier is completely dissolved and forms a homogeneous oil phase with the camellia oil. Keep warm at 75°C for later use.

[0036] While keeping both the aqueous and oil phases at 75°C, slowly and uniformly pour the oil phase into the aqueous phase, and simultaneously turn on the homogenizer, setting the homogenization speed to 4500 rpm and homogenizing for 5 minutes to form a fine and uniform primary emulsion.

[0037] Cool the colostrum to 42°C with stirring. Take a small amount of glycerol in advance and grind and disperse the chelate into a uniform slurry. Add this slurry and phenoxyethanol / ethylhexylglycerin preservative to the cooled colostrum and stir at 300 rpm for 15 minutes to ensure uniform mixing.

[0038] Slowly add an aqueous solution of arginine (0.12g of arginine was dissolved in an appropriate amount of deionized water beforehand) to the resulting mixture while stirring. Monitor the pH value in real time until the pH value reaches 6.0. Then add the remaining amount of deionized water to the total amount of the formula, continue stirring for 10 minutes, and cool to room temperature to obtain a moisturizing and soothing lotion.

[0039] Example 3 Weigh the following components by weight percentage: 12.0% high-purity cold-pressed camellia oil; 5.0% bamboo leaf flavonoid-purslane alkaloid chelate; 4.0% glycerol; 2.0% trehalose; 0.5% panthenol; 2.5% polyglycerol-3-methyl glucoside distearate; 1.5% cetearyl glucoside; 0.3% acrylates / C10-30 alkanol acrylate crosspolymer; 0.05% disodium EDTA; 0.8% p-hydroxyacetophenone / octyl glycol compound preservative; 0.15% triethanolamine (for adjusting pH to 6.2); deionized water to 100%.

[0040] The preparation steps are the same as in Example 2, except that the pH adjuster is replaced by triethanolamine (pre-diluted with an appropriate amount of deionized water before addition) instead of arginine, and the final pH value is adjusted to 6.2.

[0041] Example 4 Weigh the following components by weight percentage: 10.0% high-purity cold-pressed camellia oil; 4.0% bamboo leaf flavonoid-purslane alkaloid chelate; 6.0% glycerin; 2.0% butylene glycol; 1.0% betaine; 0.15% sodium hyaluronate; 3.0% cetearyl oleate; 1.5% sorbitan oleate; 1.0% cetearyl alcohol; 0.15% carbomer; 0.08% xanthan gum; 0.05% disodium EDTA; 0.6% phenoxyethanol / ethylhexylglycerin compound preservative; 0.15% arginine (for adjusting pH to 6.0); deionized water to 100%.

[0042] Add the prescribed amounts of glycerin, butylene glycol, betaine, sodium hyaluronate, carbomer, xanthan gum, and disodium EDTA to deionized water in sequence. After stirring and dispersing, heat to 75°C and stir at 350 rpm for 25 minutes until completely dissolved to form a homogeneous and transparent aqueous phase. Keep warm at 75°C for later use.

[0043] Mix the high-purity cold-pressed camellia oil, cetearyl oleate, sorbitan oleate, and cetearyl alcohol in the specified amounts, heat to 75°C, and stir at 250 rpm for 15 minutes until completely melted and a homogeneous oil phase is formed. Keep warm at 75°C for later use.

[0044] While keeping both the aqueous and oil phases at 75°C, the oil phase is slowly and uniformly poured into the aqueous phase. At the same time, the homogenizer is turned on, the homogenization speed is set to 5000 rpm, and the homogenization is carried out for 5 minutes to form a fine and uniform primary emulsion.

[0045] Cool the colostrum to 40°C with stirring. Take a small amount of glycerol in advance and grind and disperse the chelate into a uniform slurry. Add this slurry and phenoxyethanol / ethylhexylglycerin preservative to the cooled colostrum and stir at 250 rpm for 20 minutes.

[0046] Slowly add an aqueous solution of arginine (pre-dissolve 0.15g of arginine in an appropriate amount of deionized water) while stirring, adjust the pH to 6.0, add deionized water to the total volume, continue stirring for 10 minutes, and cool to room temperature to obtain a moisturizing and soothing face cream.

[0047] Example 5 Weigh the following components by weight percentage: 5.0% high-purity cold-pressed camellia oil; 2.0% light bamboo leaf flavonoid-purslane alkaloid chelate prepared in Example 1; 3.0% glycerol; 4.0% butylene glycol; 0.2% sodium hyaluronate; 0.5% panthenol; 0.8% PEG-40 hydrogenated castor oil; 0.1% carbomer; 0.1% hydroxyethyl cellulose; 0.05% disodium EDTA; 0.5% phenoxyethanol / ethylhexylglycerin compound preservative; 0.10% triethanolamine (used to adjust pH to 6.0); deionized water to 100%.

[0048] Add the prescribed amounts of glycerin, butylene glycol, sodium hyaluronate, panthenol, carbomer, hydroxyethyl cellulose, and disodium EDTA to deionized water in sequence. After stirring and dispersing, heat to 75°C and stir at 300 rpm for 20 minutes to form a homogeneous and transparent aqueous phase. Keep warm at 75°C for later use.

[0049] Mix the high-purity cold-pressed camellia oil and PEG-40 hydrogenated castor oil according to the formula, heat to 75°C, stir at 200 rpm for 10 minutes to form a homogeneous oil phase, and keep warm at 75°C for later use.

[0050] While keeping both the aqueous and oil phases at 75°C, slowly pour the oil phase into the aqueous phase, and simultaneously turn on the homogenizer, setting the homogenization speed to 4000 rpm and homogenizing for 3 minutes.

[0051] The system was cooled to 40°C with stirring. A small amount of deionized water was taken beforehand to disperse the chelate obtained in Example 1 evenly. This dispersion was added to the system along with the phenoxyethanol / ethylhexylglycerin preservative, and stirred at 300 rpm for 15 minutes.

[0052] Slowly add triethanolamine aqueous solution (pre-dilute 0.1g of triethanolamine with an appropriate amount of deionized water) while stirring, adjust the pH value to 6.0, add deionized water to the total volume, continue stirring for 10 minutes, and cool to room temperature to obtain the moisturizing and soothing essence.

[0053] Comparative Example 1 The difference from Example 2 is that the chelate prepared in Example 1 is replaced with an equal mass of unchelated physical mixture. This physical mixture is made by directly mixing the total flavonoid extract of Lophatherum gracile prepared in Example 1 with the alkaloid extract of Portulaca oleracea at a mass ratio of 1:1. The remaining formulation components and preparation steps are exactly the same as in Example 2.

[0054] Comparative Example 2 The difference from Example 2 is that high-purity cold-pressed camellia oil is not added at all, but is replaced with an equal mass of caprylic / capric triglycerides. The remaining formulation components and preparation steps are exactly the same as in Example 2.

[0055] Comparative Example 3 The difference from Example 2 is that no bamboo leaf flavonoid-purslane alkaloid chelate is added, while the rest of the formulation components and preparation steps are exactly the same as in Example 2.

[0056] Stability test: The moisturizing and soothing emulsion samples prepared in Example 2 were dispensed into sealed glass bottles and stored in a refrigerator at 4°C, an incubator at 25°C, and an incubator at 40°C, respectively. Samples were taken on days 0, 15, 30, and 60 to test the appearance, pH value, and flavonoid content (determined by high-performance liquid chromatography). The specific test results are as follows: Under storage conditions at 4℃, the sample remained uniformly milky white on day 30, with no stratification or precipitation, and a pH of 6.01 (initial value 6.02), with a flavonoid content retention rate of 98.2%; on day 60, the sample was still uniformly milky white, with a pH of 5.98, and a flavonoid content retention rate of 96.5%.

[0057] Under storage conditions at 25℃, the sample was uniformly milky white on day 30, with a pH of 5.99 and a flavonoid retention rate of 95.8%; on day 60, it was uniformly milky white, with a pH of 5.95 and a flavonoid retention rate of 92.4%.

[0058] Under accelerated storage conditions at 40℃, the sample was uniformly milky white on day 30, with a pH of 5.96 and a flavonoid retention rate of 91.3%; on day 60, the sample was uniformly milky white, with a pH of 5.92 and a flavonoid retention rate of 85.7%.

[0059] The results above show that the appearance and pH value of the skin care products of the present invention remain highly stable under different storage conditions. Even after 60 days of storage under accelerated conditions at 40°C, the flavonoid content retention rate is still as high as 85%, indicating that the chelation system of the present invention endows the product with excellent stability of active ingredients.

[0060] In vitro transdermal test: In vitro transdermal absorption was evaluated using a modified Franz diffusion cell method. The abdominal skin of isolated SD rats was used as the transdermal barrier, with the stratum corneum facing the supply cell and the dermis facing the receiving cell. The receiving solution was phosphate buffer solution at pH 7.4. The effective diffusion area of ​​the diffusion cell was 1.77 cm², and the volume of the receiving cell was 6.5 mL.

[0061] Accurately weigh 0.5 g each of the emulsion from Example 2 and the physical mixture emulsion from Comparative Example 1, and evenly apply them to the skin surface of the supply tank. The diffusion tank is placed in a constant temperature water bath at 37°C, and the magnetic stirring speed is 300 rpm. At 2, 4, 8, 12, and 24 hours after the start of the experiment, 0.5 mL samples are taken from the receiving tank, and an equal amount of fresh receiving liquid is added simultaneously. The contents of bamboo leaf flavonoids and purslane alkaloids in the receiving liquid at each time point are determined by high performance liquid chromatography (HPLC). The cumulative permeation per unit area at each time point is calculated, and the cumulative permeation rate over 24 hours is also calculated. The experimental results are as follows: For Lophatherum gracile flavonoids, the 24-hour cumulative permeability of the chelate group in Example 2 was 28.5% (±2.3%), while that of the physical mixture group in Comparative Example 1 was only 17.8% (±1.9%). The permeability of the chelate group was about 60.1% higher than that of the physical mixture group.

[0062] For purslane alkaloids, the 24-hour cumulative permeability of the chelate group in Example 2 was 32.7% (±3.1%), while that of the physical mixture group in Comparative Example 1 was 20.4% (±2.5%). The permeability of the chelate group was about 60.3% higher than that of the physical mixture group.

[0063] The above results show that the transdermal absorption performance of the active ingredients is significantly improved after low-temperature molecular chelation treatment, effectively solving the common technical problem of poor transdermal absorption of natural plant extracts.

[0064] Moisturizing efficacy test: Thirty healthy volunteers (12 males and 18 females, aged 22-45 years) were selected and randomly divided into three groups of 10 each. Each group applied one of the following lotions to a 4cm x 4cm area on the inner forearm: Example 2 lotion, Comparative Example 2 lotion (without camellia oil), or a commercially available brand of moisturizing lotion (as a positive control). The application rate was 2.0 mg / cm². During the experiment, the test area was kept dry and free of other skincare products. Before application, the stratum corneum moisture content was measured using a Corneometer CM825, and the transepidermal water loss (TEWL) was measured using a Tewameter TM300 as the baseline. These indicators were measured again at 2, 4, 8, 12, and 24 hours after application. The increase rate of stratum corneum moisture content (relative to baseline) and the decrease rate of TEWL at 24 hours were calculated. The specific experimental results are as follows: Regarding the immediate increase in skin stratum corneum moisture content, the rate was 68.3% (±5.1%) in Example 2, 42.7% (±4.5%) in Comparative Example 2 (without camellia oil), and 55.2% (±4.8%) in the commercially available moisturizing lotion control group. The immediate moisturizing effect of Example 2 was significantly better than that of the two control groups.

[0065] Regarding the reduction rate of 24-hour transepidermal water loss (TEWL), the reduction rate was 21.6% (±3.2%) in Example 2, 9.8% (±2.1%) in Comparative Example 2, and 14.5% (±2.7%) in the commercially available moisturizing lotion control group. Example 2 showed significantly stronger skin barrier repair and water retention capabilities.

[0066] The above results fully demonstrate that the combination of high-purity cold-pressed camellia oil and the chelate of bamboo leaf flavonoids and purslane alkaloids produces a significant synergistic effect, with both excellent immediate moisturizing and long-lasting barrier repair effects.

[0067] Soothing and anti-inflammatory efficacy test: The anti-inflammatory efficacy of the EpiKutis in vitro 3D epidermal model was evaluated. The model was treated with 0.5% sodium dodecyl sulfate (SLS) solution for 24 hours to induce skin barrier damage and inflammation. Successfully modeled models were randomly divided into four groups: a control group (no drug administration), Example 2 group, Comparative Example 1 group (physical mixing), and Comparative Example 3 group (no chelate). Each group was treated with the corresponding test substance for 24 hours at a dose of 25 μL / cm². After treatment, the culture supernatant was collected, and the levels of inflammatory factors IL-1α and IL-8 were detected using enzyme-linked immunosorbent assay (ELISA). Simultaneously, the skin barrier index ET50 (the time required for the fluorescent yellow dye to reach half of its steady-state permeability) was determined using the fluorescent yellow dye penetration method. The results are as follows: Regarding the inhibition rate of inflammatory factors, the inhibition rate of IL-1α in Example 2 group was 56.7% (±4.2%), and the inhibition rate of IL-8 was 52.3% (±3.8%); the inhibition rate of IL-1α in Comparative Example 1 group was 38.4% (±3.5%), and the inhibition rate of IL-8 was 35.1% (±3.2%); the inhibition rate of IL-1α in Comparative Example 3 group was 12.5% ​​(±2.1%), and the inhibition rate of IL-8 was 10.8% (±1.9%).

[0068] Regarding the skin barrier index ET50 value, the average time was 4.85 hours (±0.32 hours) in Example 2 group, 3.62 hours (±0.28 hours) in Comparative Example 1 group, 2.51 hours (±0.19 hours) in Comparative Example 3 group, and 2.12 hours (±0.15 hours) in the model control group.

[0069] The data above show that Example 2 exhibited inhibition rates of 56.7% and 52.3% against inflammatory factors IL-1α and IL-8, respectively, significantly higher than the physical mixing group and the blank group. Simultaneously, the ET50 value increased to 4.85 hours, approaching normal skin levels, indicating that the skincare product of this invention can significantly inhibit inflammatory responses and effectively enhance skin barrier function. The synergistic anti-inflammatory and soothing effects of the chelated bamboo leaf flavonoids and purslane alkaloids were greatly enhanced. Furthermore, when combined with camellia oil, the barrier repair effect was further strengthened, achieving a significant synergistic effect.

[0070] In summary, this invention provides a moisturizing and soothing skincare product containing camellia oil and its preparation method. Low-temperature molecular chelation technology is applied to the synergistic stabilization construction of bamboo leaf flavonoids and purslane alkaloids, successfully obtaining a structurally stable bamboo leaf flavonoid-purslane alkaloid chelate with high transdermal absorption and synergistic enhancement of anti-inflammatory and antioxidant activities. This chelate is then compounded with high-purity cold-pressed camellia oil in the optimal ratio, achieving a systemic synergistic effect of moisturizing and locking in moisture, soothing and anti-inflammatory, antioxidant scavenging of free radicals, and barrier repair.

[0071] Specifically, through a low-temperature molecular chelation process, stable hydrogen bonds and coordination bonds are formed between the phenolic hydroxyl groups in the flavonoid molecules of *Lophatherum gracile* and the nitrogen atoms in the alkaloid molecules of *Portulaca oleracea* under mild conditions of 4-25℃. This fundamentally solves the common industry problem of poor stability and easy oxidation and inactivation of natural plant active ingredients. Stability tests confirm that after 60 days of storage under accelerated conditions at 40℃, the flavonoid content retention rate of the chelate is still as high as 85%, while the retention rate of the conventional physical mixing group is less than 70%. The chelated structure endows the active complex with better hydrophilic-lipophilic balance, significantly improving the transdermal absorption efficiency of active ingredients. In vitro transdermal tests show that the 24-hour cumulative permeability of flavonoids and alkaloids in the chelate group is increased by about 60% and 60% respectively compared with the physical mixing group, solving the problem of natural plant extraction. Addressing the key bottleneck of poor transdermal absorption, high-purity cold-pressed camellia oil, rich in oleic acid, linoleic acid, and vitamin E, exhibits a significant synergistic effect when combined with chelates. Moisturizing tests show that the product of this invention increases the immediate stratum corneum moisture content by 68.3% and reduces TEWL by 21.6% after 24 hours, both significantly superior to control products without camellia oil and commercially available control products. Anti-inflammatory tests show that the product of this invention inhibits IL-1α and IL-8 by 56.7% and 52.3%, respectively, and increases the skin barrier index ET50 to 4.85 hours, approaching the level of normal skin. The preparation process of this invention is low-temperature and gentle throughout, avoiding thermal degradation of active ingredients, making it environmentally friendly and highly compatible with existing cosmetic production equipment. It can achieve large-scale production without special equipment, showing broad industrialization prospects.

[0072] Based on a comprehensive review of all experimental data and comparative results, this invention overcomes the industry shortcomings of existing plant extract skincare products, such as poor stability, low transdermal absorption rate, and limited efficacy. It demonstrates significant technological advancements and industry advantages in multiple dimensions, including active ingredient stability, transdermal absorption efficiency, immediate moisturizing ability, long-lasting barrier repair function, and anti-inflammatory and soothing effects, providing an efficient, stable, and safe innovative solution for the field of sensitive skin care.

[0073] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present invention. Any simple modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. A moisturizing and soothing skincare product containing camellia oil, characterized in that, It contains the following components by mass percentage: Camellia oil 5.0%-15.0%; Lophatherum gracile flavonoids-purslane alkaloid chelate 1.0%-8.0%; Moisturizer content: 3.0%-12.0%; Emulsifier 1.0%-5.0%; Thickener 0.1%-1.5%; Preservatives: 0.1%-1.0%; Chelating agent 0.01%-0.1%; pH adjuster 0.01%-2.0%; The remainder is deionized water; The camellia oil mentioned is high-purity cold-pressed camellia oil, with an oleic acid content of not less than 75%, a linoleic acid content of not less than 8%, and a vitamin E content of not less than 80 mg / kg; The chelate of flavonoids from Lophatherum gracile and alkaloids from Portulaca oleracea was prepared by the following method: total flavonoid extract from Lophatherum gracile and alkaloid extract from Portulaca oleracea were mixed at a mass ratio of 1:0.5-2.0, and stirred for 1-6 hours at a temperature of 4-25℃ and a pH of 6.0-7.

5. After purification by dialysis or ultrafiltration, the chelate was freeze-dried.

2. The moisturizing and soothing skincare product containing camellia oil according to claim 1, characterized in that, The total flavonoid content of the Lophatherum gracile extract is not less than 50%, and the total alkaloid content of the Portulaca oleracea alkaloid extract is not less than 20%.

3. A moisturizing and soothing skincare product containing camellia oil according to claim 1, characterized in that, The moisturizer is selected from one or more of glycerin, butylene glycol, propylene glycol, sodium hyaluronate, trehalose, betaine, and panthenol.

4. A moisturizing and soothing skincare product containing camellia oil according to claim 1, characterized in that, The emulsifier is selected from one or more of cetearyl oleate, sorbitan oleate, polyglycerol-3-methyl glucoside distearate, cetearyl glucoside, and PEG-40 hydrogenated castor oil.

5. A moisturizing and soothing skincare product containing camellia oil according to claim 1, characterized in that, The thickener is selected from one or more of carbomer, xanthan gum, acrylic / C10-30 alkanol acrylate crosspolymers, and hydroxyethyl cellulose.

6. A moisturizing and soothing skincare product containing camellia oil according to claim 1, characterized in that, The preservative is selected from one or more of phenoxyethanol, ethylhexylglycerin, p-hydroxyacetophenone, and octyl glycol; the chelating agent is disodium EDTA.

7. A moisturizing and soothing skincare product containing camellia oil according to claim 1, characterized in that, The pH adjuster is selected from one or more of arginine, triethanolamine, sodium hydroxide, and citric acid, and is used to adjust the pH value of the skin care product to 5.5-6.

5.

8. The moisturizing and soothing skincare product containing camellia oil according to claim 1 and its preparation method, characterized in that, The preparation method of the total flavonoid extract of Lophatherum gracile is as follows: take dried Lophatherum gracile, crush it through a 40-60 mesh sieve, add 60%-80% ethanol solution at a material-to-liquid ratio of 1:10-20, extract with ultrasonic assistance at 40-50℃ for 30-60 minutes, filter, concentrate the filtrate under reduced pressure to recover ethanol, load the concentrate onto a macroporous adsorption resin column, wash with water to remove sugar impurities, then elute with 50%-70% ethanol, collect the eluent, concentrate under reduced pressure, freeze dry to obtain the total flavonoid extract of Lophatherum gracile.

9. A moisturizing and soothing skincare product containing camellia oil according to claim 1, and its preparation method, characterized in that, The preparation method of the purslane alkaloid extract is as follows: Take the dried aerial parts of purslane, crush them through a 40-60 mesh sieve, add a 60%-80% acidic ethanol solution with a pH of 2-3 at a material-to-liquid ratio of 1:8-15, extract with ultrasonic assistance at 35-45℃ for 40-80 minutes, filter, concentrate the filtrate under reduced pressure to recover ethanol, adjust the pH of the concentrate to 9-10, extract with chloroform or ethyl acetate 2-3 times, combine the organic phases, concentrate under reduced pressure, freeze dry to obtain the purslane alkaloid extract.

10. A method for preparing a moisturizing and soothing skincare product containing camellia oil, the method being used to prepare the moisturizing and soothing skincare product containing camellia oil as described in any one of claims 1-9, characterized in that, The method includes the following steps: S1: Add the formulated amounts of humectant, thickener, and chelating agent to deionized water, heat to 70-80℃, and stir until completely dissolved to form an aqueous phase; S2: Mix the prescribed amounts of camellia oil and emulsifier, heat to 70-80℃, and stir until homogeneous to form an oil phase; S3: Under homogenization conditions, slowly add the oil phase to the aqueous phase, homogenize at 3000-6000 rpm for 3-8 minutes to form the primary emulsion; S4: Cool the colostrum to 40-45℃, add the prescribed amount of bamboo leaf flavonoid-purslane alkaloid chelate and preservative, and stir well; S5: Adjust the pH of the system to 5.5-6.5 with a pH adjuster, add deionized water to the full volume, stir well, and cool to room temperature to obtain the skin care product.

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