A bio-fertilizer for alleviating fruit drop of morus australis and application thereof

Abstract

The present application belongs to the technical field of plant cultivation, and particularly relates to a biological fertilizer for relieving fruit drop of Taiwan long fruit mulberry and application. The biological fertilizer comprises animal manure, soybean meal powder, humic acid, peat soil, coconut bran, bran, fermentation liquor of a compound microbial agent, seaweed extract powder, amino acid chelated iron and borax; wherein the compound microbial agent comprises Bacillus amyloliquefaciens and Pseudomonas aeruginosa. The above biological fertilizer is balanced in nutrients, is beneficial to enhancing the resistance of the mulberry to adversity and reducing fruit drop caused by stress; in addition, the Bacillus amyloliquefaciens and the Pseudomonas aeruginosa are used synergistically, and the two jointly build a healthy rhizosphere micro-ecology, thereby guaranteeing nutrient supply for fruit development from the root source. Therefore, the technical scheme of the present application can not only significantly reduce the fruit drop rate, but also improve the single fruit weight, soluble solid content and anthocyanin content of mulberry, and improve the flavor and color of the fruit.

Description

Technical Field

[0001] This invention belongs to the field of plant cultivation technology, specifically relating to a bio-fertilizer and its application for alleviating fruit drop in Taiwan's long-fruited mulberry. Background Technology

[0002] Fruit mulberry is a general term for a large category of mulberry tree varieties intended for mulberry production. Among them, the Taiwan long-fruit mulberry ( Morus macroura Due to its vigorous growth, early maturity, sweet taste, caterpillar-like shape, and rich nutritional value, the Taiwanese long-fruited mulberry is increasingly favored by mulberry growers. However, the cultivation of this variety suffers from severe fruit drop, which has consistently hindered the industry's development.

[0003] The main reasons for fruit drop in Taiwanese long-fruited mulberry include: 1. Physiological fruit drop, an internal cause that cannot be completely avoided but can be mitigated. The first physiological fruit drop (after flowering): mainly due to poor pollination and fertilization or cessation of embryonic development. The second physiological fruit drop (during the young fruit enlargement period): intense competition for nutrients between the fruit and new shoots and leaves, causing weaker fruits to fall off due to "starvation." This is the main cause of fruit drop in long-fruited mulberry. Pre-harvest fruit drop (during maturity): ethylene is produced during fruit ripening, and an abscission layer forms on the fruit stalk. 2. Nutritional imbalance, excessive nitrogen: excessive vegetative growth competes with the fruit for carbohydrates, while insufficient potassium, calcium, and boron intensifies the conflict between fruit and shoots. 3. Pests and diseases: diseases such as sclerotinia rot (white fruit disease) are devastating, causing diseased fruits to turn white and fall off. Brown spot and other diseases damage the leaves, indirectly leading to fruit drop.

[0004] Currently, patents addressing fruit drop in Taiwan's long-fruited mulberry are relatively rare, primarily focusing on cultivation and management techniques. For example, patent CN106212149 A by Qin Zhengqiang reports a planting method for Taiwan's long-fruited mulberry, mainly controlling fruit drop through measures such as proper site selection and land preparation, planting, integrated water and fertilizer management, and proper pruning and shaping. Patent CN114271124A by Zheng Zhangyun et al. mainly controls fruit drop by physically removing one bud and one leaf when the mulberry tree is 30% open, thereby regulating hormone distribution. However, these technical solutions suffer from drawbacks such as limited operability and insignificant fruit drop mitigation effects. Patent CN108739029A by Zhou Zhengguo reports a planting method for multi-season production of long-fruited mulberry, achieving multi-season fruiting through physical bud removal combined with chemical treatment and the application of different nitrogen, phosphorus, and potassium fertilizer ratios. This patented method relies excessively on the application of chemical agents and fertilizers for mulberry production, making it difficult to widely promote and apply in the current context of people increasingly pursuing green and healthy diets. Therefore, how to provide an efficient, green, and safe method to alleviate fruit drop is a problem that urgently needs to be solved in this field. Summary of the Invention

[0005] The purpose of this invention is to provide a bio-fertilizer and its application for alleviating fruit drop in Taiwan's long-fruited mulberry. It can not only effectively alleviate fruit drop, but also increase the weight of a single fruit, the content of soluble solids in the fruit, and the content of anthocyanins, and it is green and safe.

[0006] This invention provides a bio-fertilizer for alleviating fruit drop in Taiwan's long-fruited mulberry trees. The bio-fertilizer comprises the following substances by weight: Animal manure 30-40 parts, soybean meal powder 10-15 parts, humic acid 5-10 parts, peat soil 10-20 parts, coconut coir 10-20 parts, wheat bran 10-20 parts, fermentation liquid of compound microbial agent 2-4 parts, seaweed extract powder 2-4 parts, amino acid chelated iron 0.5-1 part and borax 0.5-1 part; The compound microbial agent includes: Bacillus amyloliquefaciens and Pseudomonas aeruginosa.

[0007] Preferably, the animal excrement includes: well-rotted cow manure and well-rotted sheep manure; The mass ratio of decomposed cow manure to decomposed sheep manure in the animal excrement is 3-4:1.

[0008] Preferably, the compound microbial agent is inoculated into the culture medium for aerobic fermentation to obtain the fermentation broth of the compound microbial agent; In the compound bacterial agent, the mass ratio of Bacillus amyloliquefaciens to Pseudomonas aeruginosa is 1-2:1-2; The effective viable counts of *Bacillus amyloliquefaciens* and *Pseudomonas aeruginosa* were ≥1×10⁻⁶ respectively. 7 CFU / mL.

[0009] Preferably, when conducting aerobic fermentation, the inoculum amount of the compound microbial agent is 2%-4% of the culture medium mass; The aerobic fermentation is carried out at a temperature of 32-37℃ for 3-4 days.

[0010] Preferably, the culture medium uses water as a solvent and comprises the following components: peanut cake 10-12 g / L, brown sugar 15-20 g / L, fish meal 20-25 g / L, yeast extract 3-5 g / L, calcium carbonate 2-5 g / L, potassium dihydrogen phosphate 0.1-0.5 g / L, dipotassium hydrogen phosphate 0.5-1 g / L, magnesium sulfate heptahydrate 1-2 g / L, salt 1-3 g / L, potassium sulfonate 10-20 g / L, manganese sulfate 0.001-0.005 g / L, copper sulfate pentahydrate 0.0001-0.00015 g / L, and ferrous sulfate heptahydrate 0.0001-0.00015 g / L.

[0011] This invention provides a method for preparing the bio-fertilizer described in the above technical solution, comprising the following steps: Animal manure, soybean meal powder, humic acid, peat moss, coconut coir, and wheat bran are pulverized and stirred evenly to obtain an organic carrier matrix. The organic carrier matrix, fermentation broth of compound microbial agent, seaweed extract powder, amino acid chelated iron, and borax are mixed evenly to obtain a mixture. The mixture is aged at 20-30℃ for 24-48 hours to obtain bio-fertilizer.

[0012] This invention provides the application of the bio-fertilizer described in the above technical solution in the Taiwan long-fruited mulberry tree, wherein the application includes at least one of the following (1)-(4): (1) Alleviate fruit drop; (2) Increase the weight of a single fruit; (3) Increase the soluble solids content of the fruit; (4) Increase the anthocyanin content of the fruit.

[0013] This invention provides a method for alleviating fruit drop in mulberry trees, comprising the following steps: The bio-fertilizer described in the above technical solution is applied to mulberry trees to alleviate fruit drop.

[0014] Preferably, the application is carried out by ring application or hole application, with an application rate of 0.8-1.5 kg per mature mulberry tree.

[0015] The preferred variety is the long-fruited mulberry from Taiwan.

[0016] Beneficial effects: This invention provides a bio-fertilizer for alleviating fruit drop in Taiwan mulberry trees. By weight, the bio-fertilizer comprises the following substances: 30-40 parts animal manure, 10-15 parts soybean meal powder, 5-10 parts humic acid, 10-20 parts peat moss, 10-20 parts coconut coir, 10-20 parts wheat bran, 2-4 parts fermentation broth of a compound microbial agent, 2-4 parts seaweed extract powder, 0.5-1 part amino acid chelated iron, and 0.5-1 part borax. The compound microbial agent comprises Bacillus amyloliquefaciens and Pseudomonas aeruginosa.

[0017] Compared with the prior art, the present invention has the following advantages: (1) Balanced nutrition and enhanced stress resistance: The bio-fertilizer provided by this invention combines organic and inorganic components, strengthens the calcium (strengthening cell walls), boron (promoting pollen tube elongation and sugar transport), and potassium (fruit expansion and sweetening) elements that are closely related to fruit development and stress resistance. Combined with seaweed extract and amino acids, it can effectively balance tree nutrition, enhance the mulberry tree's resistance to adverse conditions (such as hot and dry winds and sudden rain), and reduce fruit drop caused by stress.

[0018] (2) Synergistic regulation of microorganisms: This invention uses Bacillus amyloliquefaciens and Pseudomonas aeruginosa in synergy. Bacillus amyloliquefaciens can solubilize potassium, phosphorus, and fix nitrogen, and secrete extracellular polysaccharides to improve soil aggregate structure; Pseudomonas aeruginosa can produce antibacterial substances and plant growth stimulants, inhibit pathogens, and stimulate root growth. Therefore, the two work together to construct a healthy rhizosphere microecology, ensuring the nutrient supply for fruit development from the root.

[0019] (3) Safety, environmental protection, and quality improvement: In the process of preparing bio-fertilizer, this invention adopts a secondary fermentation technology. The preparation process does not require harsh conditions such as high-temperature and high-pressure sterilization, making it easy to industrialize and ensuring good stability. At the same time, this invention mainly uses biological and organic components, significantly reducing the use of chemically synthesized growth regulators and fertilizers, eliminating the risk of residues, and conforming to the direction of green agriculture development. Therefore, when the bio-fertilizer provided by this invention is applied to plants, it can not only significantly reduce the fruit drop rate, but also increase the weight of a single mulberry fruit, the content of soluble solids and anthocyanins, and improve the flavor and color of the fruit. Attached Figure Description

[0020] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0021] Figure 1 The diagram illustrates the efficacy of the bio-fertilizer provided by this invention in alleviating fruit drop in Taiwan's long-fruited mulberry. Figure 2 The effects of different treatments provided by this invention on Taiwan long-fruited mulberry. Detailed Implementation

[0022] In this invention, unless otherwise specified, the raw materials, equipment and methods used are all conventional selections.

[0023] The Bacillus amyloliquefaciens described in this invention ( Bacillus amyloliquefaciens The Pseudomonas aeruginosa (); Pseudomonas aeruginosa All of these are regular purchases.

[0024] To further illustrate the present invention, the solutions provided by the present invention will be described in detail below with reference to the accompanying drawings and embodiments, but these should not be construed as limiting the scope of protection of the present invention.

[0025] Example 1 The steps for preparing a bio-fertilizer to alleviate fruit drop in Taiwan's long-fruited mulberry are as follows: (1) Preparation of fermentation broth The liquid culture medium, using water as a solvent, includes: 12 g / L peanut cake, 20 g / L brown sugar, 20 g / L fish meal, 3 g / L yeast extract, 5 g / L calcium carbonate, 0.5 g / L potassium dihydrogen phosphate, 1 g / L dipotassium hydrogen phosphate, 2 g / L magnesium sulfate heptahydrate, 3 g / L sodium chloride (NaCl), 20 g / L potassium humate, 0.005 g / L manganese sulfate, 0.00015 g / L copper sulfate pentahydrate, and 0.00015 g / L ferrous sulfate heptahydrate. The peanut cake, brown sugar, fish meal, and yeast extract are mixed evenly with water and boiled. After cooling, calcium carbonate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, magnesium sulfate heptahydrate, sodium chloride, potassium humate, manganese sulfate, copper sulfate pentahydrate, and ferrous sulfate heptahydrate are added and stirred until dissolved to obtain the liquid culture medium. Transfer the above liquid culture medium to a fermenter and inoculate with a functional microbial agent (the functional microbial agent consists of 1×10⁶ viable cells). 7 CFU / mL of Bacillus amyloliquefaciens and an effective viable count of 1×10⁻⁶ 7 CFU / mL of *Pseudomonas aeruginosa* was mixed evenly at a mass ratio of 1:1 to obtain the liquid culture medium. The mass ratio of the liquid culture medium to the functional microbial agent was 100:2 (i.e., the inoculum amount of the functional microbial agent was 2% of the total mass of the liquid culture medium). Fermentation was carried out at a stirring rate of 40 rpm and a temperature of 32℃ for 4 days to obtain the fermentation broth. Colony counting showed that the effective viable count of the fermentation broth reached 5 × 10⁻⁶. 9 CFU / mL.

[0026] (2) Preparation of bio-fertilizer Mix well-rotted cow manure and well-rotted sheep manure evenly at a mass ratio of 4:1 to obtain animal excrement; The above-mentioned 40kg of animal manure, 10kg of soybean meal powder, 5kg of humic acid, 10kg of peat soil, 20kg of coconut coir, and 10kg of wheat bran are mechanically crushed and mixed evenly to obtain basic organic materials. 4 kg of fermentation liquid from step (1) and the above-mentioned basic organic materials are thoroughly mixed. After mixing, the organic materials can be just formed into a ball without falling apart, thus obtaining a mixture. 4 kg of seaweed extract powder, 0.5 kg of amino acid chelated iron and 1 kg of borax are added to the above mixture and stirred until uniform, thus obtaining a mixture. The mixture is aged at 25°C for 48 hours to obtain a bio-fertilizer for alleviating fruit drop in Taiwan's long-fruited mulberry.

[0027] Example 2 The steps for preparing a bio-fertilizer to alleviate fruit drop in Taiwan's long-fruited mulberry are as follows: (1) Preparation of fermentation broth The liquid culture medium, using water as a solvent, includes: 10 g / L peanut cake, 20 g / L brown sugar, 20 g / L fish meal, 3 g / L yeast extract, 5 g / L calcium carbonate, 0.5 g / L potassium dihydrogen phosphate, 1 g / L dipotassium hydrogen phosphate, 2 g / L magnesium sulfate heptahydrate, 2 g / L sodium chloride (NaCl), 20 g / L potassium humate, 0.005 g / L manganese sulfate, 0.0001 g / L copper sulfate pentahydrate, and 0.00015 g / L ferrous sulfate heptahydrate. The peanut cake, brown sugar, fish meal, and yeast extract are mixed evenly with water and boiled. After cooling, calcium carbonate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, magnesium sulfate heptahydrate, sodium chloride, potassium humate, manganese sulfate, copper sulfate pentahydrate, and ferrous sulfate heptahydrate are added and stirred until dissolved to obtain the liquid culture medium. Transfer the above liquid culture medium to a fermenter and inoculate with a functional microbial agent (the functional microbial agent consists of 1×10⁶ viable cells). 7 CFU / mL of Bacillus amyloliquefaciens and an effective viable count of 1×10⁻⁶ 7 CFU / mL of *Pseudomonas aeruginosa* was mixed evenly at a mass ratio of 1:2 to obtain the liquid culture medium. The mass ratio of the liquid culture medium to the functional microbial agent was 100:4 (i.e., the inoculum amount of the functional microbial agent was 4% of the total mass of the liquid culture medium). Fermentation was carried out at a stirring rate of 40 rpm and a temperature of 32℃ for 3 days to obtain the fermentation broth. Colony counting showed that the effective viable count of the fermentation broth reached 5.5 × 10⁻⁶. 9 CFU / mL.

[0028] (2) Preparation of bio-fertilizer Mix well-rotted cow manure and well-rotted sheep manure evenly at a mass ratio of 4:1 to obtain animal excrement; The above-mentioned animal manure (30 kg), soybean meal (15 kg), humic acid (5 kg), peat (10 kg), coconut coir (20 kg), and wheat bran (10 kg) are mechanically crushed and mixed evenly to obtain basic organic materials. 4 kg of fermentation liquid from step (1) and the above-mentioned basic organic materials are thoroughly mixed. After mixing, the organic materials can be just formed into a ball without falling apart, thus obtaining a mixture. 4 kg of seaweed extract powder, 0.5 kg of amino acid chelated iron and 1 kg of borax are added to the above mixture and stirred until uniform, thus obtaining a mixture. The mixture is aged at 25°C for 48 hours to obtain a bio-fertilizer for alleviating fruit drop in Taiwan's long-fruited mulberry.

[0029] Comparative Example 1 The only difference from Example 1 is that step (1) of Comparative Example 1 is as follows: (1) Preparation of fermentation broth The liquid culture medium, using water as a solvent, includes: 12 g / L peanut cake, 20 g / L brown sugar, 20 g / L fish meal, 3 g / L yeast extract, 5 g / L calcium carbonate, 0.5 g / L potassium dihydrogen phosphate, 1 g / L dipotassium hydrogen phosphate, 2 g / L magnesium sulfate heptahydrate, 3 g / L sodium chloride (NaCl), 20 g / L potassium humate, 0.005 g / L manganese sulfate, 0.00015 g / L copper sulfate pentahydrate, and 0.00015 g / L ferrous sulfate heptahydrate. The peanut cake, brown sugar, fish meal, and yeast extract are mixed evenly with water and boiled. After cooling, calcium carbonate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, magnesium sulfate heptahydrate, sodium chloride, potassium humate, manganese sulfate, copper sulfate pentahydrate, and ferrous sulfate heptahydrate are added and stirred until dissolved to obtain the liquid culture medium. The above liquid culture medium was transferred to a fermenter and inoculated with a functional microbial agent (i.e., an effective viable count of 1×10⁻⁶). 7 The culture medium was inoculated with *Bacillus amyloliquefaciens* at a concentration of CFU / mL. The mass ratio of liquid culture medium to functional microbial agent was 100:2 (i.e., the inoculum amount of functional microbial agent was 2% of the total mass of liquid culture medium). Fermentation was carried out at a stirring rate of 40 rpm and a temperature of 32℃ for 4 days to obtain the fermentation broth. Colony counting showed that the effective viable count of the fermentation broth reached 2 × 10⁻⁶. 9 CFU / mL.

[0030] Comparative Example 2 The only difference from Example 1 is that step (1) of Comparative Example 2 is as follows: (1) Preparation of fermentation broth The liquid culture medium, using water as a solvent, includes: 12 g / L peanut cake, 20 g / L brown sugar, 20 g / L fish meal, 3 g / L yeast extract, 5 g / L calcium carbonate, 0.5 g / L potassium dihydrogen phosphate, 1 g / L dipotassium hydrogen phosphate, 2 g / L magnesium sulfate heptahydrate, 3 g / L sodium chloride (NaCl), 20 g / L potassium humate, 0.005 g / L manganese sulfate, 0.00015 g / L copper sulfate pentahydrate, and 0.00015 g / L ferrous sulfate heptahydrate. The peanut cake, brown sugar, fish meal, and yeast extract are mixed evenly with water and boiled. After cooling, calcium carbonate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, magnesium sulfate heptahydrate, sodium chloride, potassium humate, manganese sulfate, copper sulfate pentahydrate, and ferrous sulfate heptahydrate are added and stirred until dissolved to obtain the liquid culture medium. The above liquid culture medium was transferred to a fermenter and inoculated with a functional microbial agent (i.e., an effective viable count of 1×10⁻⁶). 7 The culture medium was inoculated with *Pseudomonas aeruginosa* at a concentration of CFU / mL. The mass ratio of liquid culture medium to functional microbial agent was 100:2 (i.e., the inoculum amount of functional microbial agent was 2% of the total mass of liquid culture medium). Fermentation was carried out at a stirring rate of 40 rpm and a temperature of 32℃ for 4 days to obtain the fermentation broth. Colony counting showed that the effective viable count of the fermentation broth reached 2.8 × 10⁻⁶. 9CFU / mL.

[0031] Comparative Example 3 The only difference from Example 1 is that step (1) of Comparative Example 3 is as follows: (1) Preparation of fermentation broth The liquid culture medium was LB liquid medium, which is purchased commercially. Transfer the above liquid culture medium to a fermenter and inoculate with a functional microbial agent (the functional microbial agent consists of 1×10⁶ viable cells). 7 CFU / mL of Bacillus amyloliquefaciens and an effective viable count of 1×10⁻⁶ 7 CFU / mL of *Pseudomonas aeruginosa* was mixed evenly at a mass ratio of 1:1 to obtain the liquid culture medium. The mass ratio of the liquid culture medium to the functional microbial agent was 100:2 (i.e., the inoculum amount of the functional microbial agent was 2% of the total mass of the liquid culture medium). Aerobic fermentation was carried out at a stirring rate of 40 rpm and a temperature of 32℃ for 5 days to obtain the fermentation broth. Colony counting showed that the effective viable count of the fermentation broth reached 5.1 × 10⁻⁶. 9 CFU / mL.

[0032] Comparative Example 4 The only difference from Example 1 is that step (2) of Comparative Example 4 is as follows: (2) Preparation of bio-fertilizer 40 kg of well-rotted cow manure, 10 kg of soybean meal powder, 5 kg of humic acid, 10 kg of peat soil, 20 kg of coconut coir, and 10 kg of wheat bran are mechanically crushed and mixed evenly to obtain basic organic materials. 4 kg of fermentation liquid from step (1) and the above-mentioned basic organic materials are thoroughly mixed. After mixing, the organic materials can be just formed into a ball without falling apart, thus obtaining a mixture. 4 kg of seaweed extract powder, 0.5 kg of amino acid chelated iron and 1 kg of borax are added to the above mixture and stirred until uniform, thus obtaining a mixture. The mixture is aged at 25°C for 48 hours to obtain a bio-fertilizer for alleviating fruit drop in Taiwan's long-fruited mulberry.

[0033] Comparative Example 5 The only difference from Example 1 is that step (2) of Comparative Example 5 is as follows: (2) Preparation of bio-fertilizer Mix well-rotted cow manure and well-rotted sheep manure evenly in a mass ratio of 1:1 to obtain animal excrement; 40 kg of animal manure, 10 kg of soybean meal, 5 kg of humic acid, 10 kg of peat, 20 kg of coconut coir, and 10 kg of wheat bran are mechanically crushed and mixed evenly to obtain basic organic materials. 4 kg of fermentation liquid from step (1) and the above-mentioned basic organic materials are thoroughly mixed. After mixing, the organic materials can be just formed into a ball without falling apart, thus obtaining a mixture. 4 kg of seaweed extract powder, 0.5 kg of amino acid chelated iron and 1 kg of borax are added to the above mixture and stirred until uniform, thus obtaining a mixture. The mixture is aged at 25°C for 48 hours to obtain a bio-fertilizer for alleviating fruit drop in Taiwan's long-fruited mulberry.

[0034] Comparative Example 6 The only difference from Example 1 is that step (1) of Comparative Example 6 is as follows: (1) Preparation of fermentation broth The liquid culture medium uses water as a solvent and includes: 15 g / L peanut cake, 20 g / L brown sugar, 20 g / L fish meal, 3 g / L yeast extract, 5 g / L calcium carbonate, 0.5 g / L potassium dihydrogen phosphate, 1 g / L dipotassium hydrogen phosphate, 2 g / L magnesium sulfate heptahydrate, 3 g / L sodium chloride (NaCl), and 0.005 g / L manganese sulfate. The peanut cake, brown sugar, fish meal, and yeast extract are mixed evenly with water and boiled. After cooling, calcium carbonate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, magnesium sulfate heptahydrate, sodium chloride, and manganese sulfate are added and stirred until dissolved to obtain the liquid culture medium. Transfer the above liquid culture medium to a fermenter and inoculate with a functional microbial agent (the functional microbial agent consists of 1×10⁶ viable cells). 7 CFU / mL of Bacillus amyloliquefaciens and an effective viable count of 1×10⁻⁶ 7 CFU / mL of *Pseudomonas aeruginosa* was mixed evenly at a mass ratio of 1:1 to obtain the liquid culture medium. The mass ratio of the liquid culture medium to the functional microbial agent was 100:2 (i.e., the inoculum amount of the functional microbial agent was 2% of the total mass of the liquid culture medium). Fermentation was carried out at a stirring rate of 40 rpm and a temperature of 32℃ for 4 days to obtain the fermentation broth. Colony counting showed that the effective viable count of the fermentation broth reached 5.4 × 10⁻⁶. 9 CFU / mL.

[0035] Comparative Example 7 Regularly purchase ordinary ternary compound fertilizer with an NPK ratio of 15:15:15.

[0036] Field trials Experimental location: 1000 meters from the green corridor on Yehai Avenue, Xiuying District, Haikou City, Hainan Province (mulberry planting base). The mulberry variety is "Taiwan Long Fruit Mulberry" and the trees are 5 years old.

[0037] The bio-fertilizers used in Examples 1-2 and Comparative Examples 1-7 were used as verification subjects. Nine mulberry trees were randomly selected for each bio-fertilizer treatment. All plants were managed routinely in the same environment. Fertilizers were applied twice during the verification process: once at the beginning of the flowering period (February 15, 2025) and once at the beginning of the young fruit enlargement period (February 25, 2025). Treatment 1: Each mulberry tree was irradiated with 1.2 kg of the bio-fertilizer from Example 1 (the corresponding image during the experiment is shown). Figure 1 ).

[0038] Treatment 2: Apply 1.2 kg of the bio-fertilizer from Example 2 to each mulberry tree in a ring.

[0039] Treatment 3: Apply 1.2 kg of the bio-fertilizer from Comparative Example 1 to each mulberry tree in a ring.

[0040] Treatment 4: Apply 1.2 kg of the bio-fertilizer from Comparative Example 2 to each mulberry tree in a ring.

[0041] Treatment 5: Apply 1.2 kg of the bio-fertilizer from Comparative Example 3 to each mulberry tree in a ring.

[0042] Treatment 6: Apply 1.2 kg of the bio-fertilizer from Comparative Example 4 to each mulberry tree in a ring.

[0043] Treatment 7: Apply 1.2 kg of the bio-fertilizer from Comparative Example 5 to each mulberry tree in a ring.

[0044] Treatment 8: Apply 1.2 kg of the bio-fertilizer from Comparative Example 6 to each mulberry tree in a ring.

[0045] Treatment 9: Apply 1.2 kg of the bio-fertilizer from Comparative Example 7 to each mulberry tree in a ring.

[0046] Treatment 10: No bio-fertilizers are applied.

[0047] After fertilization on February 25, 2025, three Taiwan long-fruited mulberry trees (two branches from each tree were marked) were randomly selected from each treatment, totaling six branches. The number of fruits on each branch was investigated (average value, rounded to three decimal places). On March 20, the number of fruits on each of the six marked branches was investigated again (average value, rounded to three decimal places), and the fruit drop rate was calculated (rounded to two decimal places). The results are shown in Table 1 and [Table data missing]. Figure 2 As shown in A in the diagram.

[0048] Table 1. Fruit drop rate under different treatments

[0049] As shown in Table 1, when the bio-fertilizer prepared in the examples was applied to *Mulberry hainanensis*, the fruit drop rate was only 23.47%-27.36%, while the fruit drop rate was as high as 79.49% without any fertilizer. Therefore, treatments 1 and 2 significantly reduced the fruit drop rate of *Mulberry hainanensis*. P <0.05) is lower than that of treatment 3-treatment 10, therefore the bio-fertilizer provided by the present invention is beneficial to alleviating the fruit drop of Taiwan long-fruited mulberry.

[0050] During the peak ripening period of the mulberries, mature mulberries were harvested from different locations and parts for each treatment. The weight of a single fruit, soluble solids content, and anthocyanin content were measured. For each indicator, 50 mulberries were randomly selected for measurement, with each treatment consisting of 50 mulberries (i.e., the mean of 50 mulberries). Each treatment was replicated three times, and the average value was calculated. The results are shown in Table 2 and... Figure 2 As shown in BD.

[0051] Table 2 Effects of different treatments on the quality of mulberry fruit

[0052] Based on the values ​​in Table 2, it can be seen that compared with treatments 3-10, the bio-fertilizer in the water of treatments 1-2 is more beneficial to improving the quality of Taiwan long-fruited mulberry, specifically including: increasing the single fruit weight, increasing the soluble solids content and anthocyanin content of Taiwan long-fruited mulberry. That is, compared with treatments 3-8, the single fruit weight and soluble solids content in treatments 1-2 are significantly increased. P <0.05); Although the increase in anthocyanin content was not significant, it still increased the anthocyanin content in the fruit to some extent.

[0053] In summary, by weight, this invention comprises 30-40 parts animal manure, 10-15 parts soybean meal powder, 5-10 parts humic acid, 10-20 parts peat moss, 10-20 parts coconut coir, 10-20 parts wheat bran, 2-4 parts fermentation broth of a compound microbial agent, 2-4 parts seaweed extract powder, 0.5-1 parts amino acid chelated iron, and 0.5-1 parts borax. The compound microbial agent includes *Bacillus amyloliquefaciens* and *Pseudomonas aeruginosa*. The combination of organic and inorganic components strengthens nutrients closely related to fruit development and stress resistance, enhances the mulberry tree's resistance to adverse conditions, and reduces fruit drop caused by stress. Simultaneously, the synergistic use of *Bacillus amyloliquefaciens* and *Pseudomonas aeruginosa* jointly constructs a healthy rhizosphere microecology, ensuring nutrient supply for fruit development from the root. The resulting fertilizer is more environmentally friendly and highly efficient.

[0054] Although the above embodiments have provided a detailed description of the present invention, they are only some embodiments of the present invention, and not all embodiments. People can obtain other embodiments based on these embodiments without creative effort, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A bio-fertilizer for alleviating fruit drop in Taiwan's long-fruited mulberry trees, characterized in that, The bio-fertilizer comprises the following substances in parts by weight: Animal manure 30-40 parts, soybean meal powder 10-15 parts, humic acid 5-10 parts, peat soil 10-20 parts, coconut coir 10-20 parts, wheat bran 10-20 parts, fermentation liquid of compound microbial agent 2-4 parts, seaweed extract powder 2-4 parts, amino acid chelated iron 0.5-1 part and borax 0.5-1 part; The compound microbial agent includes: Bacillus amyloliquefaciens and Pseudomonas aeruginosa.

2. The bio-fertilizer according to claim 1, characterized in that, The animal excrement includes: well-rotted cow manure and well-rotted sheep manure; The mass ratio of decomposed cow manure to decomposed sheep manure in the animal excrement is 3-4:

1.

3. The bio-fertilizer according to claim 1, characterized in that, The compound microbial agent is inoculated into the culture medium and subjected to aerobic fermentation to obtain the fermentation broth of the compound microbial agent; In the compound bacterial agent, the mass ratio of Bacillus amyloliquefaciens to Pseudomonas aeruginosa is 1-2:1-2; The effective viable counts of *Bacillus amyloliquefaciens* and *Pseudomonas aeruginosa* were ≥1×10⁻⁶ respectively. 7 CFU / mL.

4. The bio-fertilizer according to claim 3, characterized in that, When conducting aerobic fermentation, the inoculum size of the compound microbial agent should be 2%-4% of the culture medium mass. The aerobic fermentation is carried out at a temperature of 32-37℃ for 3-4 days.

5. The bio-fertilizer according to claim 3 or 4, characterized in that, The culture medium uses water as a solvent and includes the following components: peanut cake 10-12 g / L, brown sugar 15-20 g / L, fish meal 20-25 g / L, yeast extract 3-5 g / L, calcium carbonate 2-5 g / L, potassium dihydrogen phosphate 0.1-0.5 g / L, dipotassium hydrogen phosphate 0.5-1 g / L, magnesium sulfate heptahydrate 1-2 g / L, salt 1-3 g / L, potassium humate 10-20 g / L, manganese sulfate 0.001-0.005 g / L, copper sulfate pentahydrate 0.0001-0.00015 g / L, and ferrous sulfate heptahydrate 0.0001-0.00015 g / L.

6. The method for preparing the bio-fertilizer according to any one of claims 1-5, characterized in that, Includes the following steps: Animal manure, soybean meal powder, humic acid, peat moss, coconut coir, and wheat bran are pulverized and stirred evenly to obtain an organic carrier matrix. The organic carrier matrix, fermentation broth of compound microbial agent, seaweed extract powder, amino acid chelated iron, and borax are mixed evenly to obtain a mixture. The mixture is aged at 20-30℃ for 24-48 hours to obtain bio-fertilizer.

7. The application of the bio-fertilizer according to any one of claims 1-5 in the Taiwan long-fruited mulberry tree, wherein the application includes at least one of the following (1)-(4): (1) Alleviate fruit drop; (2) Increase the weight of a single fruit; (3) Increase the soluble solids content of the fruit; (4) Increase the anthocyanin content of the fruit.

8. A method for alleviating fruit drop in mulberry trees, characterized in that, Includes the following steps: The bio-fertilizer described in any one of claims 1-5 is applied to mulberry trees to alleviate fruit drop.

9. The method according to claim 8, characterized in that, Apply by ring application or hole application, with an application rate of 0.8-1.5 kg per mature mulberry tree.

10. The method according to claim 8 or 9, characterized in that, The mulberry varieties mentioned include: Taiwan long-fruited mulberry.

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