Microbial composition, complex microbial inoculant and application thereof
By using a compound microbial composition of Bacillus amyloliquefaciens, Bacillus brevis, and Trichoderma harzianum, the problems of rice blast and Aspergillus contamination during rice seed production and storage have been solved, achieving full life-cycle protection, improving seed quality, and reducing environmental pollution.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- BEIJING LANTU TECH CO LTD
- Filing Date
- 2025-11-06
- Publication Date
- 2026-06-23
Smart Images
Figure SMS_1 
Figure SMS_2
Abstract
Description
Technical Field
[0001] This invention relates to the field of microbial technology, and in particular to a microbial composition, compound bacterial agent, and its application. Background Technology
[0002] Rice is an important food crop, and the quality of its seeds directly affects food security and farmers' income. However, rice seeds are susceptible to contamination by rice blast and aflatoxin during production and storage, leading to decreased germination rates, deterioration in quality, and even the production of toxic substances that endanger human and animal health.
[0003] Currently, the control of rice blast and aflatoxin contamination mainly relies on chemical pesticides, such as carbendazim and triadimefon. While these chemical pesticides have some control effects, they also pose the following risks: 1) Environmental pollution and pesticide residues: The use of chemical pesticides pollutes soil, water sources, and air, and remains in agricultural products, harming human health. 2) Pathogen resistance: Long-term use of chemical pesticides can lead to pathogen resistance, reducing control effectiveness. 3) Toxicity to non-target organisms: Chemical pesticides not only kill pathogens but also harm beneficial microorganisms and other non-target organisms, disrupting the ecological balance. Therefore, developing safe, efficient, and environmentally friendly biological control methods is of great significance.
[0004] In the prior art, CN 116814506 A discloses a compound microbial agent, its preparation method, and its application in controlling rice blast and / or promoting rice growth. The compound microbial agent includes *Streptomyces glaucus* and *Bacillus amyloliquefaciens*. CN 113005050 A discloses a compound microbial agent for reducing *Aspergillus flavus* infection and contamination in grain and oil crops, its preparation method, and its application. The compound microbial agent includes *Bacillus amyloliquefaciens*, *Enterobacter ludwigii*, and *Trichoderma koningii*.
[0005] However, the aforementioned compound microbial agents are only effective against single rice blast or aflatoxin contamination. They cannot achieve simultaneous prevention and control of rice blast during rice seed production and aflatoxin contamination during storage using the same compound microbial agent. In other words, they cannot achieve protection of rice seeds throughout their entire life cycle from production to storage. Summary of the Invention
[0006] To address the aforementioned technical challenges, the present invention first provides a microbial composition comprising Bacillus amyloliquefaciens, Bacillus brevis, and Trichoderma harzianum.
[0007] Preferably, the Bacillus amyloliquefaciens has the accession number CGMCC No. 35162;
[0008] And / or the accession number of the aforementioned Bacillus shortness of breath is CGMCC No. 35161;
[0009] And / or the accession number of the aforementioned Trichoderma harzianum is CGMCC No. 42123.
[0010] The *Bacillus amyloliquefaciens* strain of this invention was screened from paddy field soil and deposited on July 9, 2025, at the China General Microbiological Culture Collection Center (CGMCC). The address of the depository is: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, 100101, China. The classification and naming are: *Bacillus amyloliquefaciens* (…). Bacillus amyloliquefaciens (The accession number is CGMCC No.35162).
[0011] The *Bacillus brevis* strain of this invention was screened from rice rhizosphere soil and deposited on July 9, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at Institute of Microbiology, Chinese Academy of Sciences, No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, 100101, China. Its classification is *Bacillus brevis* (…). Brevibacillus brevis (The accession number is CGMCC No.35161).
[0012] The *Trichoderma harzianum* strain of this invention was screened from rice rhizosphere soil. This strain was deposited on July 9, 2025, at the China General Microbiological Culture Collection Center (CGC-CMEC), located at Institute of Microbiology, Chinese Academy of Sciences, No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, 100101, China. Its classification and nomenclature are: *Trichoderma harzianum* (…). Trichoderma Harzian (The accession number is CGMCC No.42123).
[0013] Through extensive screening of bacterial strains, this invention has discovered that a microbial composition combining Bacillus amyloliquefaciens, Bacillus brevis, and Trichoderma harzianum can simultaneously prevent and control rice blast during rice seed production and aflatoxin contamination during storage. The three strains work synergistically to achieve protection of rice seeds throughout their entire life cycle, from production to storage, which is beneficial for improving seed germination rate and quality.
[0014] In some embodiments, the microbial composition includes fermentation broth, fermentation broth concentrate, or dried product of Bacillus amyloliquefaciens, Bacillus brevis, and Trichoderma harzianum.
[0015] In some embodiments, the fermentation broth of the Bacillus amyloliquefaciens is the fermentation broth obtained by culturing the Bacillus amyloliquefaciens in LB medium;
[0016] And / or, the fermentation broth of the Bacillus brevis is the fermentation broth after culturing the Bacillus brevis in LB medium;
[0017] And / or, the fermentation broth of Trichoderma harzianum is the fermentation broth after Trichoderma harzianum has been cultured in PDB medium.
[0018] Preferably, the fermentation broth of Bacillus amyloliquefaciens is prepared by inoculating Bacillus amyloliquefaciens into LB medium and fermenting it at 28°C to 32°C and pH 6.5 to 7.5. More preferably, the fermentation time is 24 to 48 hours.
[0019] Preferably, the fermentation broth of the Bacillus brevis is prepared by inoculating the Bacillus brevis into LB medium and fermenting it at 30°C to 35°C and pH 7.0 to 7.5. More preferably, the fermentation time is 18 to 36 hours.
[0020] Preferably, the fermentation broth of *Trichoderma harzianum* is prepared by inoculating *Trichoderma harzianum* into PDB medium and fermenting it at 25°C–28°C and pH 5.5–6.5. More preferably, the fermentation time is 48–72 hours.
[0021] In some embodiments, the ratio of viable Bacillus amyloliquefaciens, Bacillus brevis, and Trichoderma harzianum in the microbial composition is (0.2~6):(0.5~3):1.
[0022] Preferably, the ratio of the effective viable counts of Bacillus amyloliquefaciens, Bacillus brevis, and Trichoderma harzianum is (0.25~2):(0.5~2):1.
[0023] Preferably, the ratio of the effective viable counts of Bacillus amyloliquefaciens, Bacillus brevis, and Trichoderma harzianum is (0.25~0.5):(0.5~1):1;
[0024] More preferably, the ratio of the effective viable counts of Bacillus amyloliquefaciens, Bacillus brevis, and Trichoderma harzianum is (0.25~0.3):(0.5~0.8):1.
[0025] The present invention further discovers that, at the above effective live bacteria ratio, the microbial composition has higher activity, higher stability and longer shelf life, and the control effect on rice blast and aflatoxin contamination is greatly improved.
[0026] In some embodiments, the microbial composition also includes a protectant.
[0027] In the specific implementation process, a protective agent can be added to the fermentation broth, fermentation broth concentrate, or dried product.
[0028] In some embodiments, the protective agent includes at least one of trehalose, skim milk powder, glycerin, dextrin, mannitol, and sucrose.
[0029] Preferably, the mass of the protective agent accounts for 3% to 10% of the total mass of the microbial composition.
[0030] Preferably, the protective agent includes trehalose, skim milk powder, and glycerin.
[0031] The present invention has found that the protective agent using the above-mentioned formulation can better improve the activity and stability of microorganisms and their metabolites in the microbial composition of the present invention, thereby further enhancing the prevention and control effect against rice blast and aflatoxin contamination.
[0032] Furthermore, the present invention provides a compound microbial agent comprising the aforementioned microbial composition.
[0033] Furthermore, the present invention provides a product, wherein the product is selected from at least one of seed preservatives, antibacterial agents, antifungal agents, and pharmaceuticals; the product comprises the aforementioned microbial composition or the aforementioned compound microbial agent.
[0034] Furthermore, the present invention provides the application of the aforementioned microbial composition or the aforementioned compound microbial agent in the prevention and control of rice blast fungus and / or Aspergillus flavus.
[0035] Furthermore, the present invention provides the application of the aforementioned microbial composition or the aforementioned compound microbial agent in the preparation of products.
[0036] Preferably, the product is selected from at least one of seed preservatives, antibacterial agents, antifungal agents, and pharmaceuticals.
[0037] Preferably, the product is used to prevent and control rice blast fungus and / or aflatoxin.
[0038] Furthermore, the present invention provides a method for improving seed storage quality, comprising: soaking seeds in the microbial composition, the compound microbial agent, or an aqueous solution of the product.
[0039] Preferably, the seeds are dried and stored after soaking.
[0040] Preferably, the soaking time is 30-60 minutes.
[0041] In practice, the method described above is used to control rice blast fungus and / or Aspergillus flavus. Therefore, all plant seeds affected by rice blast fungus and / or Aspergillus flavus can have their storage quality improved using the above method.
[0042] Furthermore, the present invention provides a method for the prevention and control of rice blast and / or aflatoxin contamination, comprising: soaking seeds in the microbial composition, or the compound microbial agent or the aqueous solution of the product, drying them, and then sowing them.
[0043] And / or the control method includes: spraying the microbial composition, the compound microbial agent, or an aqueous solution of the product onto the plants.
[0044] The above-described prevention and control methods of the present invention are applicable to all plants affected by rice blast fungus and / or Aspergillus flavus.
[0045] Preferably, the seed is a rice seed.
[0046] Preferably, the plant is a rice plant.
[0047] Preferably, in the aqueous solution of the microbial composition, the compound microbial agent, or the product, the concentration of each microorganism is 1×10⁻⁶. 6 ~1×10 8 CFU / mL.
[0048] Compared with the prior art, the beneficial effects of the present invention are as follows:
[0049] This invention provides a microbial composition and a compound microbial agent containing the same, which can simultaneously prevent and control rice blast during rice seed production and aflatoxin contamination during storage, achieving full life-cycle protection for rice seeds from production to storage. This solves the problem in existing technologies that require the separate use of different agents to control rice blast and aflatoxin contamination. Furthermore, the compound microbial agent of this invention is safe, efficient, and environmentally friendly, and can replace chemical pesticides, reducing environmental pollution and pesticide residues. Its preparation method is simple, easy to industrialize, and has broad application prospects. Detailed Implementation
[0050] To make the objectives, technical solutions, and advantages of this invention clearer, the technical solutions of this invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of this invention, not all embodiments. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this invention. In the embodiments provided in this specification, unless specific techniques or conditions are specified, they should be performed according to the techniques or conditions described in the literature in this field, or according to the product instructions. Reagents or instruments used without specified manufacturers are all conventional products that can be purchased through legitimate channels. In the following embodiments, "parts" refers to parts by weight, and the specific unit can be g or kg, etc.
[0051] I. Materials and Methods
[0052] 1. Experimental Materials
[0053] Experimental strain: Bacillus amyloliquefaciens Bacillus amyloliquefaciens (LT-87, accession number CGMCC No. 35162), Bacillus shorthandii Brevibacillus brevis(LT-88, accession number CGMCC No. 35161), Trichoderma harzianum Trichoderma harzianum (LT-89, accession number CGMCC No. 42123). All of the above strains are deposited at the China General Microbiological Culture Collection Center.
[0054] Pathogen: Rice blast fungus Magnaporthe rice Highly pathogenic strain GDC-1, Aspergillus flavus Aspergillus yellow Toxigenic strain AF-01.
[0055] Test plants and sites:
[0056] The rice variety 'Longjing 3023' was used in all trials.
[0057] Rice blast experiment: Pot experiments were conducted in an insect-proof greenhouse.
[0058] Aspergillus flavus test: Pot experiments were conducted in an insect-proof greenhouse until the end of the rice grain-filling stage (yellow ripening stage).
[0059] 2. Preparation of bacterial suspension and compound bacterial agent
[0060] Single bacterial suspensions: Each strain was inoculated separately into PDB liquid medium. The medium was prepared as follows: 200g of peeled potatoes were cut into pieces, boiled for 30 minutes, filtered, and the water volume was increased to 1L. 20g / L glucose was added, and the pH was adjusted to 5.6±0.2 before sterilization. The medium was cultured at 30℃ and 180 rpm with shaking until the late logarithmic growth phase. Cells / spores were collected by centrifugation and resuspended in sterile physiological saline. Finally, the concentrations of *Bacillus amyloliquefaciens* LT-87 and *Bacillus brevis* LT-88 were uniformly adjusted to 1×10⁻⁶. 7 CFU / mL, the spore concentration of Trichoderma harzianum LT-88 was adjusted to 1×10⁻⁶ CFU / mL. 7 spores / mL.
[0061] Compound microbial agent: The prepared single-strain suspensions of Bacillus amyloliquefaciens LT-87, Bacillus brevis LT-88, and Trichoderma harzianum LT-89 were mixed in gradient ratios of 6:3:1, 4:2:1, 2:2:1, 1:1:1, 1:2:2, and 1:2:4, respectively, to obtain the compound microbial agent. The concentration was adjusted so that the concentration of microorganisms in the final compound microbial agent was consistent with that of the single-strain suspensions.
[0062] 3. Preparation of pathogenic fungal spore suspension
[0063] Rice blast fungus and Aspergillus flavus were inoculated separately onto PDA plates, activated, and then the spores were washed with sterile water. The filtrate was filtered through four layers of sterile gauze and collected. The concentration of the rice blast fungus spore suspension was adjusted to 1×10⁻⁶ using a hemocytometer. 5 The concentration of the Aspergillus flavus spore suspension was adjusted to 1×10⁻⁶ spores / mL. 6 spores / mL, for later use.
[0064] 4. Seed treatment
[0065] Rice seeds were grouped according to the quantities in the examples and comparative examples, with the same number of seeds in each group. Each group of seeds was soaked in different single or compound microbial agents for 30 minutes. The soaked seeds were then air-dried and set aside for later use (experimental group).
[0066] Take rice seeds from the same group as the experimental group, soak them in sterile water, and then dry them for later use (control group).
[0067] 5. Rice blast fungus inoculation experiment
[0068] The seedling trays that had been treated were placed in an insect-proof greenhouse with the same light, temperature and humidity for cultivation.
[0069] Thirty days after inoculation, rice seedlings in both the experimental and control groups were uniformly sprayed with rice blast fungus suspension to simulate natural infection conditions. The incidence of rice blast fungus was then investigated post-inoculation.
[0070] 6. Aspergillus flavus inoculation experiment
[0071] During the heading stage of rice, the single or compound microbial agent described in point “2” above (experimental group) was sprayed onto the rice plants in advance, and sterile water was sprayed onto the same number of control group plants.
[0072] Three days later, Aspergillus flavus suspension was evenly sprayed onto rice panicles in both the experimental group and the same number of control groups to simulate natural infection conditions. Aspergillus flavus disease incidence was investigated after inoculation.
[0073] 7. At different stages of rice growth (seedling stage, tillering stage, heading stage, and maturity stage), the incidence of rice blast and aflatoxin contamination in each treatment group was investigated and recorded. The incidence rate and disease index were expressed as follows.
[0074] 8. Evaluation Indicators and Calculation Methods
[0075] Rice blast incidence rate = (Number of diseased leaves / Total number of leaves surveyed) × 100%
[0076] Rice blast disease index: The 0-9 grade standard is adopted, and the calculation formula is: Disease index = [Σ(number of diseased leaves at each grade × relative grade value) / (total number of leaves surveyed × highest grade value)] × 100.
[0077] Aspergillus contamination rate (%) = (Contaminated area of grains infected with Aspergillus flavus / Total area) × 100%
[0078] Aspergillus fulva disease index: Grading standards are established based on the contaminated area and severity of rice grains (e.g., Grade 0: no disease; Grade 1: 1-25% of grains have lesions; Grade 2: 26-50%; Grade 3: 51-75%; Grade 4: 76-100%). The calculation formula is the same as that for rice blast disease index.
[0079] Prevention and control effect = [(Disease index of control group - Disease index of treatment group) / Disease index of control group] × 100%
[0080] Calculation of control effect: The expected control effect (E) was calculated using the Colby formula and compared with the actual observed control effect (O). Colby formula: E = X + Y + Z -
(X·Y) / 100+(X·Z) / 100+(Y·Z) / 100
[0081] 9. Data Analysis
[0082] All experiments were performed in triplicate, and data are expressed as mean ± standard deviation. One-way ANOVA was performed using SPSS 26.0 software, and multiple comparisons were conducted using Duncan's new multiple range method. A p-value < 0.05 was considered statistically significant. II. Specific Implementation Methods
[0084] Examples 1-5
[0085] Prepare the inoculant according to the proportions in Table 1, and conduct the seed soaking and pathogen control experiments according to the materials and methods section. Evaluate the results according to the methods described in the evaluation index and calculation method section. The experimental results are shown in Table 1.
[0086] In addition, based on the control effect data of using a single microbial agent in Table 1, the expected control effect (E) of each ratio of the mixed microbial agent was calculated by substituting it into the Colby formula: E = X + Y + Z-
(X·Y) / 100+(X·Z) / 100+(Y·Z) / 100
[0087] Table 1
[0088]
[0089] Table 2
[0090]
[0091] As shown in Table 2, the simultaneous use of Bacillus amyloliquefaciens, Bacillus brevis, and Trichoderma harzianum in this invention produced a synergistic effect in the control of rice blast and Aspergillus flavus infection. The effect was optimal when the ratio of Bacillus amyloliquefaciens, Bacillus brevis, and Trichoderma harzianum was 1:2:4.
[0092] Furthermore, the combination of these three microorganisms can prevent rice blast and aspergillosis during the rice production stage, and also has a preventive effect against aspergillosis contamination during storage (the anti-aspergillosis effect during the growth process has been verified, so the test process for anti-aspergillosis activity during storage is omitted here). Those skilled in the art will understand that it can also prevent aspergillosis infection during storage, thus the compound microbial agent of the present invention can achieve full life-cycle protection of rice seeds from production to storage.
[0093] The microbial agent of the present invention can be used not only for seed soaking, but also for spraying at different growth stages of rice.
[0094] The results of the above examples and comparative examples collectively demonstrate that the compound microbial agent, formulated from Bacillus amyloliquefaciens, Bacillus brevis, and Trichoderma harzianum in a specific ratio, exhibits significant synergistic control effects against rice blast and aflatoxin on both live rice plants and seeds. Its effectiveness far surpasses that of individual strains used alone, exceeding the theoretical additive effect. This compound microbial agent shows promising application prospects.
[0095] Furthermore, the following embodiments provide a compound microbial agent containing a protectant.
[0096] In the following examples, the LB medium formulation is as follows: 10 g / L tryptone, 5 g / L yeast extract, 10 g / L sodium chloride, and 15 g / L agar. To prepare, dissolve the above components in deionized water, adjust the pH to 7.0-7.2, and then bring the volume to 1 L.
[0097] Example 6
[0098] This embodiment provides a compound microbial agent, the preparation method of which is as follows:
[0099] Bacillus amyloliquefaciens CGMCC No. 35162 was inoculated into LB medium and fermented at 30℃ and pH 7.0 for 24 h to obtain Bacillus amyloliquefaciens fermentation broth. Bacillus brevis CGMCC No. 35161 was inoculated into LB medium and fermented at 32℃ and pH 7.2 for 18 h to obtain Bacillus brevis fermentation broth. Trichoderma harzianum CGMCC No. 42123 was inoculated into PDB medium and fermented at 26℃ and pH 6.0 for 48 h to obtain Trichoderma harzianum fermentation broth.
[0100] A compound bacterial agent was prepared by mixing 50 parts of Bacillus amyloliquefaciens fermentation broth, 30 parts of Bacillus brevis fermentation broth, 20 parts of Trichoderma harzianum fermentation broth, and 5 parts of a protectant (trehalose).
[0101] Example 7
[0102] This embodiment provides a compound microbial agent, the preparation method of which differs from that of Example 6 only in that:
[0103] A compound bacterial agent was prepared by mixing 40 parts of Bacillus amyloliquefaciens fermentation broth, 30 parts of Bacillus brevis fermentation broth, 20 parts of Trichoderma harzianum fermentation broth, and 5 parts of a protectant (trehalose).
[0104] Example 8
[0105] This embodiment provides a compound microbial agent, the preparation method of which differs from that of Example 6 only in that:
[0106] A compound bacterial agent was prepared by mixing 60 parts of Bacillus amyloliquefaciens fermentation broth, 20 parts of Bacillus brevis fermentation broth, 10 parts of Trichoderma harzianum fermentation broth, and 5 parts of a protectant (trehalose).
[0107] Example 9
[0108] This embodiment provides a compound microbial agent, the preparation method of which differs from that of Example 6 only in that:
[0109] The protective agent (trehalose) was replaced with an equal amount of the protective agent composition; the protective agent composition in this embodiment was prepared by mixing trehalose, skim milk powder and glycerin in a mass ratio of 2:2:1.
[0110] Example 10
[0111] This embodiment provides a compound microbial agent, the preparation method of which differs from that of Example 6 only in that:
[0112] The protective agent (trehalose) was replaced with an equal amount of the protective agent composition; the protective agent composition in this embodiment was prepared by mixing mannitol, glycerol and sucrose in a mass ratio of 2:2:1.
[0113] Tests have shown that Examples 6-10 above can also prevent and control rice blast and Aspergillus flavus infection, achieving full life-cycle protection of rice seeds from production to storage. Moreover, the compound microbial agent containing trehalose, skim milk powder and glycerin protectant has a better prevention and control effect.
[0114] The above are merely embodiments of the present invention and are not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principle of the present invention should be included within the scope of the claims of the present invention.
Claims
1. A microbial composition, characterized in that, The composition is composed of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) Bacillus amyloliquefaciens ), Bacillus shortiflora ( Brevibacillusbrevis ) and Trichoderma harzianum ( Trichodermaharzianum )composition, The accession number of the Bacillus amyloliquefaciens is CGMCC No. 35162, the accession number of the Bacillus brevis is CGMCC No. 35161, and the accession number of Trichoderma harzianum is CGMCC No. 42123.
2. The microbial composition according to claim 1, characterized in that, In the microbial composition, the ratio of the effective viable counts of Bacillus amyloliquefaciens, Bacillus brevis, and Trichoderma harzianum is (0.2~6):(0.5~3):
1.
3. The microbial composition according to claim 2, characterized in that, In the microbial composition, the ratio of the effective viable counts of Bacillus amyloliquefaciens, Bacillus brevis, and Trichoderma harzianum is (0.25~2):(0.5~2):
1.
4. The microbial composition according to claim 2, characterized in that, The ratio of the effective viable counts of the *Bacillus amyloliquefaciens*, the *Bacillus brevis*, and the *Trichoderma harzianum* is (0.25~0.5):(0.5~1):
1.
5. The microbial composition according to claim 2, characterized in that, The ratio of the effective viable counts of the *Bacillus amyloliquefaciens*, the *Bacillus brevis*, and the *Trichoderma harzianum* is (0.25~0.3):(0.5~0.8):
1.
6. A compound microbial agent, characterized in that, The compound microbial agent uses the microbial composition of any one of claims 1 to 5 as its active ingredient.
7. The compound microbial agent according to claim 6, characterized in that, The compound microbial agent includes the fermentation broth, fermentation broth concentrate, or dried product of the Bacillus amyloliquefaciens, the Bacillus brevis, and the Trichoderma harzianum.
8. The compound microbial agent according to claim 6, characterized in that, The compound microbial agent also includes a protectant.
9. The compound microbial agent according to claim 8, characterized in that, The protective agent includes at least one of trehalose, skim milk powder, glycerin, dextrin, mannitol, and sucrose.
10. The compound microbial agent according to claim 8, characterized in that, The protective agent accounts for 3% to 10% of the total mass of the microbial composition.
11. A pharmaceutical preparation, characterized in that, The agent is selected from at least one of seed preservatives, antibacterial agents, and antifungal agents, and the agent uses the microbial composition of any one of claims 1 to 5 as the active ingredient.
12. The use of the microbial composition of any one of claims 1 to 5, the compound microbial agent of any one of claims 6 to 10, or the agent of claim 11 in the control of rice blast fungus and / or aflatoxin.
13. A method for improving seed storage quality, characterized in that, include: The seeds are soaked in an aqueous solution of the microbial composition according to any one of claims 1 to 5, the compound microbial agent according to any one of claims 6 to 10, or the agent according to claim 11.
14. The method according to claim 13, characterized in that, The seeds are rice seeds.
15. A method for controlling rice blast and / or aflatoxin contamination, characterized in that, include: Soak the seeds in an aqueous solution of the microbial composition according to any one of claims 1 to 5, the compound microbial agent according to any one of claims 6 to 10, or the agent according to claim 11, then dry them before sowing; or The microbial composition of any one of claims 1 to 5, the compound microbial agent of any one of claims 6 to 10, or the aqueous solution of the agent of claim 11 is sprayed onto the plant.
16. The prevention and control method according to claim 15, characterized in that, The seeds are rice seeds.