Livestock manure fermentation bio-organic fertilizer and preparation method thereof

Abstract

The present application relates to the technical field of organic fertilizer, in particular to a kind of livestock manure fermentation biological organic fertilizer and preparation method thereof.The preparation raw material of the organic fertilizer includes the following components by weight: pretreated livestock manure 60-80 parts, compound fermentation inoculum 0.5-1 part, nano SiO2-DA-MBA 4-6 parts, mixed traditional Chinese medicine residue 10-15 parts, polysaccharide extract 5-9 parts.

Description

Technical Field

[0001] This invention relates to the field of organic fertilizer technology, specifically to a fermented bio-organic fertilizer made from livestock manure and its preparation method. Background Technology

[0002] With the rapid development of large-scale and intensive livestock and poultry farming, the amount of livestock manure generated during the breeding process has increased dramatically year by year. The indiscriminate dumping or direct return of untreated livestock manure to the fields causes environmental pollution. Preparing bio-organic fertilizer from livestock manure can achieve the harmless treatment and resource utilization of livestock manure. Currently, research and application of bio-organic fertilizer based on animal manure still have certain limitations. Chinese patent application number CN202211064481.3 discloses a bio-organic fertilizer, its preparation method, and its application. The provided bio-organic fertilizer can effectively increase rice yield and effectively reduce the content of available cadmium in the soil and the cadmium content in brown rice, thereby improving the production safety of rice cultivation. Chinese patent application number CN202210597904.1 discloses a bio-organic fertilizer using garden waste as a matrix and its preparation method. The prepared bio-organic fertilizer can promote plant growth and / or prevent diseases. However, the nutrient content of the bio-organic fertilizers prepared by the above inventions is relatively low, affecting the yield and quality of crops. Summary of the Invention

[0003] To address the shortcomings of existing technologies, this invention proposes a fermented bio-organic fertilizer made from livestock manure and its preparation method.

[0004] This invention is achieved through the following technical solution: A fermented bio-organic fertilizer made from livestock manure, the raw materials of which include the following components by weight: 60-80 parts of pretreated livestock manure, 0.5-1 part of compound fermentation agent, 4-6 parts of nano-SiO2-DA-MBA, 10-15 parts of mixed Chinese herbal medicine residue, and 5-9 parts of polysaccharide extract.

[0005] Furthermore, the livestock manure is selected from one or more of pig manure, cow manure, sheep manure, chicken manure, and duck manure in any proportion.

[0006] Furthermore, the pre-treated livestock manure is prepared by drying fresh livestock manure and then crushing it through a 20-mesh sieve.

[0007] Furthermore, the compound fermentation agent is composed of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Lactobacillus acidophilus, Saccharomyces cerevisiae, Aspergillus niger, and Pseudomonas aeruginosa in a viable cell ratio of 3:2:2:1:1:0.5:0.5, and the total viable cell count of the compound fermentation agent is ≥2×10⁻⁶. 10 CFU / g.

[0008] Furthermore, the mixed Chinese herbal medicine residue includes the following components in parts by weight: 1-2 parts of Isatis indigotica root residue, 2-3 parts of Lonicera japonica vine residue, 0.5-1 part of Paeonia lactiflora root residue, 1-2 parts of Chrysanthemum indicum root residue, 2-4 parts of Ilex chinensis root residue, and 0.4-0.6 parts of Viola yedoensis root residue. The residue is the residue remaining after water extraction of Chinese herbal medicine, which is dried and then pulverized through an 80-100 mesh sieve.

[0009] Furthermore, the preparation method of the nano-SiO2-DA-MBA includes the following steps: L1. Deionized water, KH550 and anhydrous ethanol were mixed at a mass ratio of 1:1:9 for 30 min to obtain a hydrolysate. Nano-SiO2 was dispersed in ethanol, and the hydrolysate was added dropwise. The mixture was stirred at 80℃ for 4-5 h, centrifuged at 8000 rpm for 15-20 min, the precipitate was washed with anhydrous ethanol and dried under vacuum to obtain modified nano-SiO2. L2. Take the modified nano-SiO2, dopamine hydrochloride (DA) and N,N'-methylenebisacrylamide (MBA) obtained in step L1, add them to DMSO and mix well. React at 60℃ and 1000 rpm in the dark for 4 h. Cool to room temperature, filter, wash and vacuum dry to obtain nano-SiO2-DA-MBA.

[0010] Furthermore, in step L1, the particle size of the nano-SiO2 is preferably 20-30 nm.

[0011] Furthermore, in step L1, the mass ratio of KH550 to nano-SiO2 is 1:3.

[0012] Furthermore, in step L1, the mass concentration of the nano-SiO2 in anhydrous ethanol is 15-20 mg / mL.

[0013] Furthermore, in step L2, the mass ratio of the modified nano-SiO2, dopamine hydrochloride, and N,N'-methylenebisacrylamide is 1:2:4.

[0014] Furthermore, in step L2, the mass concentration of the modified nano-SiO2 in DMSO is 20 mg / mL.

[0015] Furthermore, the method for preparing the polysaccharide extract includes the following steps: V1. Mix the powder of *Pleurotus ostreatus* and *Pleurotus ostreatus* at a mass ratio of 1:3-4 to obtain a mixed powder. Add the mixed powder to deionized water at a material-to-liquid ratio of 1 g:30-40 mL. Heat and extract twice at 60-80℃, 2 h each time. Combine the filtrates and concentrate under reduced pressure to 20% of the original volume. V2. After concentration under reduced pressure in step V1, add 4 times the volume of ethanol to the concentrate, let it stand at 4°C for 24 h, centrifuge at 5000-6000 rpm for 20 min, wash the precipitate with ethanol, and dry under vacuum to obtain the polysaccharide extract.

[0016] Furthermore, in step V1, the elm yellow mushroom powder and oyster mushroom powder are made by washing and drying elm yellow mushrooms and oyster mushrooms respectively, and then pulverizing them through a 60-80 mesh sieve.

[0017] Furthermore, the present invention also provides a method for preparing the fermented bio-organic fertilizer from livestock manure, comprising the following steps: S1. Take pretreated livestock manure, mixed Chinese herbal medicine residue and polysaccharide extract, mix them evenly, adjust the water content to 55-60% to obtain a mixture; take compound fermentation agent and add it to 10-15 times the weight volume of activation liquid, shake and activate at 30℃ and 120-150 rpm for 2 h to obtain activated bacterial solution; S2. Mix the activated bacterial solution with the mixed materials evenly, compost and ferment. When the temperature reaches 50℃, start turning the pile. Turn the pile once every 2 days, control the material temperature at 50-55℃ and the moisture content at 50%-55%. After 7 days, turn the pile evenly to reduce the pile temperature to 45-50℃ and the moisture content to 35%-40%, and obtain the primary fermented material. S3. Add nano-SiO2-DA-MBA evenly to the primary fermentation material, stir and mix well, turn the pile over once every 5 days, control the material temperature at 30-40℃, ferment for 10-15 days, cool, and obtain livestock manure fermented bio-organic fertilizer.

[0018] Further, in step S1, the activation solution is a sterile aqueous solution containing 2 wt% glucose and 0.5 wt% yeast extract.

[0019] Compared with the prior art, the present invention has the following beneficial effects: This invention provides a method for preparing fermented bio-organic fertilizer from livestock manure. By combining pretreated livestock manure with mixed traditional Chinese medicine residue and polysaccharide extract, sufficient carbon, nitrogen, and active substances are provided for microbial fermentation. The mixed traditional Chinese medicine residue is rich in various trace elements and organic matter; the polysaccharide extract from *Pleurotus ostreatus* and *Pleurotus ostreatus* further stimulates the proliferation of beneficial microorganisms. This invention also prepares nano-SiO2-DA-MBA. Nano-SiO2 is modified with the silane coupling agent KH550, grafted with amino groups, and cross-linked with the amino groups of dopamine hydrochloride and the double bonds of N,N'-methylenebisacrylamide to construct a three-dimensional cross-linked polymer network. This forms a polymer network with high adhesion and good biocompatibility on the surface of nano-SiO2, resulting in a composite material with good biocompatibility and slow-release dispersion. This material can act as a nutrient slow-release carrier or soil structure improver in fertilizers, facilitating the sustained absorption of nutrients by crops and promoting growth. The compound fermentation agent used in this invention is composed of a variety of functional bacteria, which can efficiently decompose macromolecular substances in livestock manure and traditional Chinese medicine residues, transforming them into small-molecule nutrients and active substances that are easily absorbed by plants. Simultaneously, the fermentation process itself can kill pathogens and parasite eggs, achieving the harmless treatment of livestock manure. The active ingredients remaining in the mixed traditional Chinese medicine residues, when applied to the soil as organic fertilizer, may have a certain inhibitory effect on some harmful bacteria in the soil, helping to improve the rhizosphere microecological environment. Combined with the polysaccharide extract's effect of promoting the proliferation of beneficial soil microorganisms, the organic fertilizer of this invention not only provides nutrients but also improves the soil and enhances the crop's resistance to adverse conditions. Attached Figure Description

[0020] Figure 1 The total nutrient content of the organic fertilizer described in Example 1 and Comparative Examples 1-4 of this invention; Figure 2 The seed germination index of the organic fertilizer described in Example 2 and Comparative Examples 1-4 of this invention; Figure 3 This invention relates to the effects of the organic fertilizer described in Example 3 and Comparative Examples 1-4 on the height of rapeseed plants. Detailed Implementation

[0021] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to specific embodiments. However, this invention is not limited to the following embodiments. It should be noted that, unless otherwise specified, all chemical reagents involved in this invention are purchased through commercial channels.

[0022] Example 1: A fermented bio-organic fertilizer made from livestock manure, the raw materials of which include the following components by weight: 80 parts of pretreated livestock manure, 1 part of compound fermentation agent, 6 parts of nano-SiO2-DA-MBA, 15 parts of mixed Chinese herbal medicine residue, and 9 parts of polysaccharide extract.

[0023] The livestock manure is a mixture of pig manure, cow manure and sheep manure in a weight ratio of 4:6:3; the pre-treated livestock manure is fresh livestock manure that has been dried and crushed and passed through a 20-mesh sieve.

[0024] The compound fermentation agent consists of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Lactobacillus acidophilus, Saccharomyces cerevisiae, Aspergillus niger, and Pseudomonas aeruginosa, with a viable cell ratio of 3:2:2:1:1:0.5:0.5. The total viable cell count of the compound fermentation agent is 2 × 10⁻⁶. 10 CFU / g.

[0025] The mixed Chinese herbal medicine residue includes the following components in parts by weight: 2 parts of Isatis root residue, 3 parts of Lonicera japonica vine residue, 1 part of Paeonia lactiflora residue, 2 parts of Chrysanthemum indicum residue, 4 parts of Ilex chinensis residue, and 0.6 parts of Viola yedoensis residue. The residue is the residue remaining after water extraction of Chinese herbal medicine, which is dried and then crushed through a 100-mesh sieve.

[0026] The preparation method of nano-SiO2-DA-MBA includes the following steps: L1. 3 g of deionized water, 3 g of KH550 and 27 g of anhydrous ethanol were mixed at a mass ratio of 1:1:9 for 30 min to obtain a hydrolysate. 9 g of 30 nm nano-SiO2 was dispersed in 450 mL of ethanol, and the hydrolysate was added dropwise. The mixture was stirred at 80 °C for 5 h, centrifuged at 8000 rpm for 20 min, the precipitate was washed with anhydrous ethanol and dried under vacuum to obtain modified nano-SiO2. L2. Take 5 g of the modified nano-SiO2 obtained in step L1, 10 g of dopamine hydrochloride (DA) and 20 g of N,N'-methylenebisacrylamide (MBA) and add them to 250 mL of DMSO. Mix well and react at 60℃ and 1000 rpm in the dark for 4 h. Cool to room temperature, filter, wash and vacuum dry to obtain nano-SiO2-DA-MBA.

[0027] The preparation method of polysaccharide extract includes the following steps: V1. Wash and dry the elm mushroom powder and oyster mushroom powder separately, then pulverize them through an 80-mesh sieve to obtain elm mushroom powder and oyster mushroom powder. Mix the elm mushroom powder and oyster mushroom powder at a mass ratio of 1:4 to obtain a mixed powder. Add the mixed powder to deionized water at a material-to-liquid ratio of 1 g:40 mL, and extract twice at 80℃ for 2 hours each time. Combine the filtrates and concentrate under reduced pressure to 20% of the original volume. V2. After concentration under reduced pressure in step V1, add 4 times the volume of ethanol to the concentrate, let it stand at 4°C for 24 h, centrifuge at 6000 rpm for 20 min, wash the precipitate with ethanol, and dry under vacuum to obtain the polysaccharide extract.

[0028] This embodiment also provides a method for preparing the fermented bio-organic fertilizer from livestock manure, including the following steps: S1. Take pretreated livestock manure, mixed Chinese herbal medicine residue and polysaccharide extract, mix them evenly, adjust the water content to 60%, and obtain a mixture; take compound fermentation agent and add it to 15 times the weight volume of activation solution, shake and activate at 30℃ and 150 rpm for 2 h to obtain activated bacterial solution; the activation solution is a sterile aqueous solution containing 2wt% glucose and 0.5wt% yeast extract; S2. Mix the activated bacterial solution with the mixed materials evenly, compost and ferment. When the temperature reaches 50℃, start turning the pile. Turn the pile once every 2 days, control the material temperature at 55℃ and the moisture content at 55%. After 7 days, turn the pile evenly to reduce the pile temperature to 50℃ and the moisture content to 40%, and obtain the first fermented material. S3. Add nano-SiO2-DA-MBA evenly to the primary fermentation material, stir and mix well, turn the pile over once every 5 days, control the material temperature at 40℃, ferment for 15 days, cool, and obtain livestock manure fermented bio-organic fertilizer.

[0029] Example 2: A fermented bio-organic fertilizer made from livestock manure, the raw materials of which include the following components by weight: 60 parts of pretreated livestock manure, 0.5 parts of compound fermentation agent, 4 parts of nano-SiO2-DA-MBA, 10 parts of mixed Chinese herbal medicine residue, and 5 parts of polysaccharide extract.

[0030] The livestock manure is a mixture of pig manure, sheep manure and chicken manure in a weight ratio of 2:7:1; the pre-treated livestock manure is fresh livestock manure that has been dried and crushed through a 20-mesh sieve.

[0031] The compound fermentation agent consists of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Lactobacillus acidophilus, Saccharomyces cerevisiae, Aspergillus niger, and Pseudomonas aeruginosa, with a viable cell ratio of 3:2:2:1:1:0.5:0.5. The total viable cell count of the compound fermentation agent is 2 × 10⁻⁶. 10 CFU / g.

[0032] The mixed Chinese herbal medicine residue includes the following components in parts by weight: 1 part of Isatis root residue, 2 parts of Lonicera japonica vine residue, 0.5 parts of Paeonia lactiflora residue, 1 part of Chrysanthemum indicum residue, 2 parts of Ilex chinensis residue, and 0.4 parts of Viola yedoensis residue. The residue is the residue remaining after water extraction of Chinese herbal medicine, which is dried and then crushed through an 80-mesh sieve.

[0033] The preparation method of nano-SiO2-DA-MBA includes the following steps: L1. 3 g of deionized water, 3 g of KH550 and 27 g of anhydrous ethanol were stirred and mixed for 30 min to obtain a hydrolysate. 9 g of 20 nm nano-SiO2 was dispersed in 600 mL of ethanol, and the hydrolysate was added dropwise. The mixture was stirred at 80 °C for 4 h, centrifuged at 8000 rpm for 15 min, the precipitate was washed with anhydrous ethanol and dried under vacuum to obtain modified nano-SiO2. L2. Take 5 g of the modified nano-SiO2 obtained in step L1, 10 g of dopamine hydrochloride (DA) and 20 g of N,N'-methylenebisacrylamide (MBA) and add them to 250 mL of DMSO. Mix well and react at 60℃ and 1000 rpm in the dark for 4 h. Cool to room temperature, filter, wash and vacuum dry to obtain nano-SiO2-DA-MBA.

[0034] The preparation method of polysaccharide extract includes the following steps: V1. Wash and dry the elm yellow mushroom and oyster mushroom separately, then pulverize them through a 60-mesh sieve to obtain elm yellow mushroom powder and oyster mushroom powder. Mix the elm yellow mushroom powder and oyster mushroom powder at a mass ratio of 1:3 to obtain a mixed powder. Add the mixed powder to deionized water at a material-to-liquid ratio of 1 g:30 mL, and extract twice at 60℃ for 2 hours each time. Combine the filtrates and concentrate under reduced pressure to 20% of the original volume. V2. After concentration under reduced pressure in step V1, add 4 times the volume of ethanol to the concentrate, let it stand at 4°C for 24 h, centrifuge at 5000 rpm for 20 min, wash the precipitate with ethanol, and dry under vacuum to obtain the polysaccharide extract.

[0035] This embodiment also provides a method for preparing the fermented bio-organic fertilizer from livestock manure, including the following steps: S1. Take pretreated livestock manure, mixed Chinese herbal medicine residue and polysaccharide extract, mix them evenly, adjust the water content to 55%, and obtain a mixture; take compound fermentation agent and add it to 10 times the weight volume of activation solution, shake and activate at 30℃ and 120 rpm for 2 h to obtain activated bacterial solution; the activation solution is a sterile aqueous solution containing 2wt% glucose and 0.5wt% yeast extract; S2. Mix the activated bacterial solution with the mixed materials evenly, compost and ferment. When the temperature reaches 50℃, start turning the pile. Turn the pile once every 2 days, control the material temperature at 50℃ and the moisture content at 50%. After 7 days, turn the pile evenly to reduce the pile temperature to 45℃ and the moisture content to 35%, and obtain the primary fermented material. S3. Add nano-SiO2-DA-MBA evenly to the primary fermentation material, stir and mix well, turn the pile over once every 5 days, control the material temperature at 30℃, ferment for 10 days, cool, and obtain livestock manure fermented bio-organic fertilizer.

[0036] Example 3: A fermented bio-organic fertilizer made from livestock manure, the raw materials of which include the following components by weight: 70 parts of pretreated livestock manure, 0.8 parts of compound fermentation agent, 5 parts of nano-SiO2-DA-MBA, 12 parts of mixed Chinese herbal medicine residue, and 6 parts of polysaccharide extract.

[0037] The livestock manure is a mixture of cow manure, chicken manure, and duck manure in a weight ratio of 5:2:3; the pre-treated livestock manure is fresh livestock manure that has been dried and crushed through a 20-mesh sieve.

[0038] The compound fermentation agent consists of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Lactobacillus acidophilus, Saccharomyces cerevisiae, Aspergillus niger, and Pseudomonas aeruginosa, with a viable cell ratio of 3:2:2:1:1:0.5:0.5. The total viable cell count of the compound fermentation agent is 2 × 10⁻⁶. 10 CFU / g.

[0039] The mixed Chinese herbal medicine residue includes the following components in parts by weight: 1.5 parts of Isatis root residue, 2.5 parts of Lonicera japonica vine residue, 0.8 parts of Paeonia lactiflora residue, 1.5 parts of Chrysanthemum indicum residue, 3 parts of Ilex chinensis residue, and 0.5 parts of Viola yedoensis residue. The residue is the residue remaining after water extraction of Chinese herbal medicine, which is dried and then crushed through a 90-mesh sieve.

[0040] The preparation method of nano-SiO2-DA-MBA includes the following steps: L1. 3 g of deionized water, 3 g of KH550 and 27 g of anhydrous ethanol were mixed at a mass ratio of 1:1:9 for 30 min to obtain a hydrolysate. 9 g of 25 nm nano-SiO2 was dispersed in 500 mL of ethanol, and the hydrolysate was added dropwise. The mixture was stirred at 80 °C for 4.5 h, centrifuged at 8000 rpm for 18 min, the precipitate was washed with anhydrous ethanol and dried under vacuum to obtain modified nano-SiO2. L2. Take 5 g of the modified nano-SiO2 obtained in step L1, 10 g of dopamine hydrochloride (DA) and 20 g of N,N'-methylenebisacrylamide (MBA) and add them to 250 mL of DMSO. Mix well and react at 60℃ and 1000 rpm in the dark for 4 h. Cool to room temperature, filter, wash and vacuum dry to obtain nano-SiO2-DA-MBA.

[0041] The preparation method of polysaccharide extract includes the following steps: V1. Wash and dry the elm yellow mushroom and oyster mushroom separately, then pulverize them through a 70-mesh sieve to obtain elm yellow mushroom powder and oyster mushroom powder. Mix the elm yellow mushroom powder and oyster mushroom powder at a mass ratio of 1:3.2 to obtain a mixed powder. Add the mixed powder to deionized water at a material-to-liquid ratio of 1 g:35 mL, and extract twice at 70℃ for 2 hours each time. Combine the filtrates and concentrate under reduced pressure to 20% of the original volume. V2. After concentration under reduced pressure in step V1, add 4 times the volume of ethanol to the concentrate, let it stand at 4°C for 24 h, centrifuge at 5500 rpm for 20 min, wash the precipitate with ethanol, and dry under vacuum to obtain the polysaccharide extract.

[0042] This embodiment also provides a method for preparing the fermented bio-organic fertilizer from livestock manure, including the following steps: S1. Take pretreated livestock manure, mixed Chinese herbal medicine residue and polysaccharide extract, mix them evenly, adjust the water content to 58%, and obtain a mixture; take compound fermentation agent and add it to 12 times the weight volume of activation solution, shake and activate at 30℃ and 140 rpm for 2 h to obtain activated bacterial solution; the activation solution is a sterile aqueous solution containing 2wt% glucose and 0.5wt% yeast extract; S2. Mix the activated bacterial solution with the mixed materials evenly, compost and ferment. When the temperature reaches 50℃, start turning the pile. Turn the pile once every 2 days, control the material temperature at 52℃ and the moisture content at 53%. After 7 days, turn the pile evenly to reduce the pile temperature to 48℃ and the moisture content to 37%, and obtain the first fermented material. S3. Add nano-SiO2-DA-MBA evenly to the primary fermentation material, stir and mix well, turn the pile over once every 5 days, control the material temperature at 35℃, ferment for 12 days, cool, and obtain livestock manure fermented bio-organic fertilizer.

[0043] The only difference between Comparative Example 1 and Example 1 is that the residue of Isatis root is used instead of the mixed Chinese medicine residue. That is, the residue of honeysuckle vine, white peony root, wild chrysanthemum, evergreen and violet are not added, only the residue of Isatis root is added.

[0044] The only difference between Comparative Example 2 and Example 1 is that no polysaccharide extract was added.

[0045] The only difference between Comparative Example 3 and Example 1 is that nano-SiO2-DA-MBA is not added.

[0046] The only difference between Comparative Example 4 and Example 1 is that modified nano-SiO2 is used instead of nano-SiO2-DA-MBA.

[0047] Experimental Example 1: Livestock manure fermented bio-organic fertilizers prepared in Example 1 and Comparative Examples 1-4 were used. The total nutrients in the fertilizers were measured, and the results are as follows: Figure 1 As shown.

[0048] Figure 1The results showed that the organic matter content of the organic fertilizer in Example 1 was significantly better than that in Comparative Examples 1-4. Comparative Example 1 used only Isatis root residue, Comparative Example 2 did not add polysaccharide extract, Comparative Example 3 did not add nano-SiO2-DA-MBA, and Comparative Example 4 used modified nano-SiO2 to replace nano-SiO2-DA-MBA. The total nutrient content of the organic fertilizer in Comparative Examples 1-4 all decreased, indicating that the organic fertilizer prepared by the present invention has a high total nutrient content and can meet the usage requirements.

[0049] Experimental Example 2: Seed germination index (GI) was determined according to the "Organic Fertilizer" (NY / T 525-2021). The organic fertilizer prepared in Example 2 was added to deionized water at a solid-liquid ratio (m / V) of 1:10. Extraction was carried out at 25℃ with shaking for 1 h. After standing for 0.5 h, the supernatant was filtered to obtain the extract. The seed germination index (GI) was determined using radish seeds and cultured at 25℃ in the dark for 48 h. The results are as follows: Figure 2 As shown.

[0050] Figure 2 The results showed that the organic matter content of the organic fertilizer in Example 2 was significantly better than that in Comparative Examples 1-4. Comparative Example 1 used only Isatis root residue, Comparative Example 2 did not add polysaccharide extract, Comparative Example 3 did not add nano-SiO2-DA-MBA, and Comparative Example 4 used modified nano-SiO2 to replace nano-SiO2-DA-MBA. The seed germination index of Comparative Examples 1-4 all decreased, indicating that the organic fertilizer prepared by the present invention improves the seed germination index, promotes plant growth, and has high fertilizer quality.

[0051] Experimental Example 3: The organic fertilizers prepared in Example 3 and Comparative Examples 1-4 were mixed with soil at a volume ratio of 9:1. Rapeseed seedlings with a height of 6 cm were transplanted into 50cm×20cm×15cm flowerpots, each filled with 2.5 kg of soil. After 30 days of cultivation, the plant height was measured, and the results are as follows. Figure 3 As shown.

[0052] Figure 3 The results showed that the organic matter content of the organic fertilizer in Example 3 was significantly better than that in Comparative Examples 1-4. Comparative Example 1 used only Isatis root residue, Comparative Example 2 did not add polysaccharide extract, Comparative Example 3 did not add nano-SiO2-DA-MBA, and Comparative Example 4 used modified nano-SiO2 to replace nano-SiO2-DA-MBA. The rapeseed plant height in Comparative Examples 1-4 all decreased, indicating that the organic fertilizer prepared by this invention can significantly promote plant growth.

[0053] Those skilled in the art should understand that the discussion of any of the above embodiments is merely exemplary and is not intended to imply that the scope of the invention is limited to these examples; within the framework of the invention, the technical features of the above embodiments or different embodiments can also be combined, the steps can be implemented in any order, and there are many other variations of the different aspects of the invention as described above, which are not provided in detail for the sake of brevity.

Claims

1. A fermented bio-organic fertilizer made from livestock manure, characterized in that, The raw materials for preparation include the following components in parts by weight: 60-80 parts of pretreated livestock manure, 0.5-1 part of compound fermentation agent, 4-6 parts of nano-SiO2-DA-MBA, 10-15 parts of mixed Chinese herbal medicine residue, and 5-9 parts of polysaccharide extract. The preparation method of nano-SiO2-DA-MBA includes the following steps: L1. Modified nano-SiO2 was obtained by modifying nano-SiO2 with KH550; L2. Take the modified nano-SiO2 obtained in step L1, dopamine hydrochloride and N,N'-methylenebisacrylamide, add them to DMSO, mix well, react, filter, wash and dry to obtain nano-SiO2-DA-MBA; The preparation method of polysaccharide extract includes the following steps: V1. Mix the powder of elm mushroom and oyster mushroom at a mass ratio of 1:3-4 to obtain a mixed powder, add it to deionized water, heat to extract, and concentrate under reduced pressure; V2. After concentration under reduced pressure in step V1, add ethanol, let stand, centrifuge, wash, and dry to obtain the polysaccharide extract.

2. The livestock manure fermented bio-organic fertilizer according to claim 1, characterized in that, The compound fermentation agent is composed of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Lactobacillus acidophilus, Saccharomyces cerevisiae, Aspergillus niger, and Pseudomonas aeruginosa in a viable cell ratio of 3:2:2:1:1:0.5:0.5, and the total viable cell count of the compound fermentation agent is ≥2×10⁻⁶. 10 CFU / g.

3. The livestock manure fermented bio-organic fertilizer according to claim 2, characterized in that, The mixed Chinese herbal medicine residue includes the following components in parts by weight: 1-2 parts of Isatis indigotica root residue, 2-3 parts of Lonicera japonica vine residue, 0.5-1 part of Paeonia lactiflora root residue, 1-2 parts of Chrysanthemum indicum root residue, 2-4 parts of Ilex chinensis root residue, and 0.4-0.6 parts of Viola yedoensis root residue.

4. The livestock manure fermented bio-organic fertilizer according to claim 3, characterized in that, In step L1, the mass ratio of KH550 to nano-SiO2 is 1:

3.

5. The livestock manure fermented bio-organic fertilizer according to claim 4, characterized in that, In step L2, the mass ratio of the modified nano-SiO2, dopamine hydrochloride, and N,N'-methylenebisacrylamide is 1:2:

4.

6. A method for preparing fermented bio-organic fertilizer from livestock manure as described in any one of claims 1-5, characterized in that, Includes the following steps: S1. Take pretreated livestock manure, mixed Chinese herbal medicine residue and polysaccharide extract, mix them evenly, adjust the moisture content to obtain a mixture; take compound fermentation agent to activate it to obtain activated bacterial solution; S2. Mix the activated bacterial solution with the mixed materials evenly, and compost to obtain primary fermented material; S3. Add nano-SiO2-DA-MBA evenly to the primary fermentation material, stir and mix well, ferment to obtain livestock manure fermented bio-organic fertilizer.

Images