A strain producing maltogenic amylase and its application in beer brewing

CN122146512APending Publication Date: 2026-06-05SECOND INST OF OCEANOGRAPHY MNR
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SECOND INST OF OCEANOGRAPHY MNR
Filing Date
2026-01-27
Publication Date
2026-06-05

AI Technical Summary

Technical Problem

Existing industrial maltose amylases have insufficient enzyme activity under low-temperature conditions, resulting in low efficiency in low-temperature food processing and energy-saving industrial processes. Furthermore, the saccharification efficiency and fermentable sugar conversion rate are limited in traditional beer brewing, making it difficult to produce low-sugar, low-calorie, high-fermentation beer.

Method used

A new species of Gram-negative bacteria, strain D5-20, was isolated and screened from deep-sea microorganisms. This strain can secrete maltose amylase, which has the characteristics of low temperature resistance, acid and alkali resistance, salt resistance and divalent cation resistance, and maintains high activity. It is suitable for the biocatalysis of starch hydrolysis and sugar compounds in beer brewing and other processes.

Benefits of technology

It maintains high activity and good thermal stability under low temperature conditions, adapts to a wide range of temperatures and pH values, and remains stable, especially under acidic conditions and high-salt environments. It solves the bottleneck problems of saccharification efficiency and fermentable sugar conversion rate in traditional processes, and promotes the development of beer brewing technology towards controllable and designable modern food biotechnology.

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Abstract

The present application relates to the field of microorganism and beer brewing, and discloses a maltogenic amylase-producing strain and application thereof in beer brewing Christiangramia A new species strain of the genus Kishigamiella is isolated and screened from a deep-sea sample The strain can secrete maltogenic amylase, and has excellent low-temperature resistance, acid and alkali resistance, salt resistance and divalent cation resistance, and can maintain high activity under the above conditions, so that it is suitable for starch hydrolysis and related biological catalysis and conversion of sugar compounds in beer brewing and the like.
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