Efficient plastic degrading enzyme screening system based on metagenome mining and yeast surface display and application of efficient plastic degrading enzyme screening system

By using a high-efficiency screening system based on metagenomic mining and yeast surface display, combined with microfluidic droplets and flow cytometry, the problem of balancing high substrate specificity and high throughput in existing PET degrading enzyme screening has been solved, achieving efficient and accurate screening and enrichment of PET degrading enzymes.

CN122146628APending Publication Date: 2026-06-05SOUTH CHINA AGRICULTURAL UNIVERSITY
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Patent Information

Application Number
CN202610300304.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-03-12
Publication Date
2026-06-05

AI Technical Summary

Technical Problem

Existing plastic degrading enzymes have low activity and poor thermal stability. Traditional screening methods have low throughput and cannot cover difficult-to-culture bacterial communities. High-throughput screening has a high false positive rate, making it difficult to balance high substrate specificity and high-throughput screening.

Method used

A highly efficient screening system based on metagenomics mining and yeast surface display was adopted. False positives were eliminated by screening with PET microplastic substrates. Combined with microfluidic droplets, single-cell enzyme activity visualization and high-throughput flow cytometry sorting were achieved, ensuring a combination of high-specificity screening and high-throughput detection.

Benefits of technology

This method enables efficient and accurate screening of PET degrading enzymes, significantly improving screening efficiency and accuracy, and yielding highly active and specific PET degrading enzymes suitable for industrial applications.

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Abstract

The invention discloses an efficient plastic degrading enzyme screening system based on metagenome mining and yeast surface display and application of the efficient plastic degrading enzyme screening system, and relates to the technical field of synthetic biology and biological catalysis. False positive is eliminated through PET micro-plastic substrate screening, single cell enzyme activity visualization is achieved through micro-fluidic liquid drops, and flow type high-throughput sorting is combined, so that the screening efficiency of the plastic degrading enzyme is improved. High-specificity screening and high-throughput detection are coupled in series, on the premise that screening accuracy is guaranteed, rapid and efficient enrichment of the target PET degrading enzyme is achieved, the technical problem that in existing PET degrading enzyme screening, high substrate specificity and high throughput are difficult to consider at the same time is solved, the screening efficiency and practicability of the PET degrading enzyme are greatly improved, and the application prospect is wide. And the method is of great significance in breaking through the bottleneck of a PET biological recovery technology and realizing plastic closed-loop circulation.
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