Bhet hydrolase and its application in water treatment and plastic recycling

By screening novel BHET hydrolases from deep-sea bacteria, the problems of low catalytic efficiency and poor stability in existing technologies have been solved, achieving efficient degradation of BHET and expanding its application scope in water pollution treatment and plastic recycling.

CN122146653APending Publication Date: 2026-06-05SECOND INST OF OCEANOGRAPHY MNR

Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
SECOND INST OF OCEANOGRAPHY MNR
Filing Date
2026-01-13
Publication Date
2026-06-05

AI Technical Summary

Technical Problem

Existing short-chain ester hydrolases have low catalytic efficiency for the polyester plastic precursor BHET and poor stability under complex industrial conditions, making them difficult to apply effectively to water pollution treatment and plastic recycling.

Method used

A novel BHET hydrolase has been screened from deep-sea bacteria. It exhibits high specificity, demonstrates efficient degradation of short-chain ester substrates, and maintains high stability over a wide pH range, a wide temperature range, and under conditions of high concentrations of divalent cations and organic solvents.

Benefits of technology

It achieves efficient degradation of BHET in complex environments, expanding its application prospects in water pollution treatment and plastic recycling.

✦ Generated by Eureka AI based on patent content.

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Abstract

The present application relates to the field of environmental protection and resource recycling, and discloses a kind of organic solvent-resistant broad-spectrum pH stable BHET hydrolytic enzyme and its application in water treatment, plastic recycling.The present application obtains a new type of BHET hydrolytic enzyme from deep-sea bacteria, contains the amino acid sequence shown in SEQ ID NO:2 or contains the amino acid sequence (recombinant protein) except 1-23 positions (signal peptide) in SEQ ID NO:2, which shows high specificity to short-chain ester substrates and can effectively degrade polyester plastic precursor BHET.In addition, it has broad-spectrum pH, temperature adaptability, good organic solvent / divalent cation resistance.This characteristic makes it have wide application prospect in the field of water pollution treatment, plastic recycling and utilization, etc.
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