Fusarium toxin degrading enzyme genes DONepi, DONrd and use thereof

By isolating and cloning the DONepi and DONrd genes from Nocardia, the proteins they encode catalyze the isomerization and reduction of DON without the need for special coenzymes, thus solving the problem of DON contamination and achieving efficient and economical food safety assurance.

CN122146730APending Publication Date: 2026-06-05HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HUAZHONG AGRI UNIV
Filing Date
2025-12-04
Publication Date
2026-06-05

AI Technical Summary

Technical Problem

Current technologies lack effective methods for degrading deoxynivalenol (DON), a toxin that widely contaminates food and feed, posing health risks. Furthermore, existing enzyme degradation methods rely on a special coenzyme PQQ, which is costly and has limited application.

Method used

The DONepi and DONrd genes were isolated and cloned from Nocardia. The proteins they encode can catalyze the isomerization and reduction of DON without the need for special coenzymes, forming 3-epi-DON and 8-OH-3-epi-DON. The degradation process is simple and efficient.

Benefits of technology

It achieves efficient degradation of DON, reduces the toxin content in food and feed, provides food safety assurance, and the degradation enzyme is easy to express and mass-produce.

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    Figure LUKUCHJMDC3T4RDF1GMVTFAB5VU3JU76QN7IWDOV
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Abstract

The application discloses two novel fusarium toxin degrading enzyme genes DONepi , DONrd and application thereof. In-vitro experiments prove that the purified DONepi protein can independently catalyze the hydroxyl isomerization of DON at C-3 position to form 3-isomer-DON, which is completely different from the currently reported two-step catalytic reaction of isomerization of DON by two different enzymes. The purified DONrd protein can further catalyze the degradation of 3-epi-DON to reduce the ketone group at C-8 position to a hydroxyl group to form 8-hydroxy-3-isomer-DON, which is the first time to find a toxin degrading enzyme capable of further degrading the intermediate product 3-isomer-DON; meanwhile, the DONrd protein can directly catalyze the reduction of the ketone group at C-8 position of DON to form alpha / beta-8-hydroxy-DON, which is the first time to find a toxin degrading enzyme capable of acting on the ketone group at C-8 position of DON molecule.
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