A multiple nucleic acid detection system and method based on programmable DNA structure state machine and enzymatic clock stepping

Abstract

The application belongs to the technical field of biomedical detection, and particularly relates to a multiplex nucleic acid detection system and method based on programmable DNA structure state machine and enzymatic clock stepping. The application discloses a multiplex nucleic acid detection system based on programmable DNA structure state machine and enzymatic clock stepping, which comprises equipment and detection reagents (containing enzymes, signal chains, time sequence chains and multiplex primer pairs). The system connects multiple target recognition zones in series at the head and tail through a long-chain physical bottom rail, and only configures a single-color probe. The time resolution strategy is used to indicate the specific target identity by the relative time node of the fluorescent signal, so that the number limitation of traditional multi-color optical channels is broken, and higher number of targets is concurrently detected in a single-tube system.

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