A liquid supply circulation system and an optical imaging system for a living body pod

Abstract

This application provides a liquid supply and circulation system and an optical imaging system for a live organism chamber. The liquid supply and circulation system includes a first liquid storage tank; a heat exchange module comprising a semiconductor wafer, a first flow channel layer, and a second flow channel layer, with the semiconductor wafer sandwiched between the first and second flow channel layers; the first and second flow channel layers are isolated from each other; the first flow channel layer includes a first inlet and a first outlet, the first inlet communicating with the first liquid storage tank, and the first outlet supplying liquid to the live organism chamber; the second flow channel layer includes a second inlet and a second outlet; and a heat sink including a third inlet and a third outlet, the third inlet connected to the second outlet, and the third outlet connected to the second inlet, thereby creating a temperature difference across the semiconductor wafer. This enables high-precision and stable temperature control of the circulating liquid within the live organism chamber, maintaining the stable environment required for long-term imaging of the live organism.

Description

Technical Field

[0001] This application relates to the field of bioimaging detection technology, specifically to a liquid supply circulation system and an optical imaging system for a living organism chamber. Background Technology

[0002] In the field of biological live-cell optical imaging, the live-cell circulation system, as a supporting device for live-cell imaging equipment, needs to provide a stable and uniform temperature environment for the live organisms inside the live-cell chamber to ensure that the live organisms maintain good activity during long-term imaging, thereby meeting the needs of continuous observation.

[0003] Most existing temperature control and circulation systems for live organism chambers employ direct electric heating, heating the circulating liquid directly through heating elements. While this method offers rapid heating, the heating elements cannot ensure uniform heating of the circulating liquid, resulting in a rather aggressive temperature control process and difficulty in achieving a smooth temperature transition. These issues can easily lead to excessive localized temperature fluctuations within the chamber, which can adversely affect the health of live organisms such as zebrafish. Furthermore, existing temperature control and circulation systems are prone to liquid agitation and flow impacts during the liquid supply process. These agitations and impacts directly interfere with the observation stability of optical imaging, causing image shifts or blurring, and failing to meet the requirements for long-term, highly stable optical imaging of live organisms. Summary of the Invention

[0004] This application addresses the aforementioned technical problems in the existing technology. The purpose of this application is to provide a liquid supply and circulation system and an optical imaging system for a living organism chamber, which can achieve uniform and gradual temperature control of the circulating liquid, reduce temperature fluctuations and liquid flow impact, improve the stability of the living environment, and meet the requirements for long-term, high-definition optical imaging.

[0005] According to a first aspect of this application, a liquid supply and circulation system for a living chamber is provided. The liquid supply and circulation system includes: a first liquid storage tank; a heat exchange module, which includes a semiconductor wafer, a first flow channel layer, and a second flow channel layer, wherein the semiconductor wafer is sandwiched between the first flow channel layer and the second flow channel layer; the first flow channel layer and the second flow channel layer are isolated from each other, the first flow channel layer includes a first liquid inlet and a first liquid outlet, the first liquid inlet is connected to the first liquid storage tank, the first liquid outlet is used to supply liquid to the living chamber, the second flow channel layer includes a second liquid inlet and a second liquid outlet; and a heat sink, which includes a third liquid inlet and a third liquid outlet, the third liquid inlet being connected to the second liquid outlet, and the third liquid outlet being connected to the second liquid inlet, thereby creating a temperature difference on both sides of the semiconductor wafer.

[0006] In some embodiments of this application, the liquid supply circulation system further includes a power supply device for supplying power to the semiconductor wafer, the semiconductor wafer being configured to control the temperature of the liquid within the first flow channel layer based on the temperature difference.

[0007] In some embodiments of this application, there are multiple semiconductor wafers, which are arranged in an array between the first flow channel layer and the second flow channel layer, and the two working surfaces of the semiconductor wafers are respectively in close contact with the first flow channel layer and the second flow channel layer.

[0008] In some embodiments of this application, a damping structure is provided in the first flow channel layer, the damping structure being used to maintain the flow stability of the entire circulating liquid.

[0009] In some embodiments of this application, the damping structure includes a plurality of guide plates alternately arranged along the liquid inlet direction of the first flow channel layer, and the inclination direction of each guide plate is consistent with the liquid inlet direction.

[0010] In some embodiments of this application, the liquid supply circulation system further includes a three-way valve and a drive mechanism. The three-way valve includes a fifth liquid inlet, a second air inlet, and a first connection port. The drive mechanism is used to control the three-way valve to switch between the fifth liquid inlet and the second air inlet. The fifth liquid inlet is connected to the first liquid outlet, and the first connection port is connected to the liquid inlet of the living chamber.

[0011] In some embodiments of this application, the liquid supply circulation system further includes an air pump, which includes a first air inlet and a first air outlet; the first air inlet is an open interface for drawing in air; the first air outlet is connected to the second air inlet to provide an air supply to the three-way valve.

[0012] In some embodiments of this application, the radiator further includes a second liquid storage tank and a second centrifugal pump. The second liquid storage tank contains refrigerant, and the second centrifugal pump is used to drive the refrigerant to circulate between the second liquid storage tank and the second flow channel layer.

[0013] In some embodiments of this application, the liquid supply circulation system further includes a first centrifugal pump, and the first liquid storage tank includes a fourth liquid inlet and a fourth liquid outlet, wherein the fourth liquid outlet is connected to the first liquid inlet, the first centrifugal pump is used to drive the liquid in the first liquid storage tank to enter the first flow channel layer through the fourth liquid outlet, and the fourth liquid inlet is connected to the liquid outlet of the living chamber to form a liquid circulation loop in the living chamber.

[0014] In some embodiments of this application, an optical imaging system is provided, which includes a living chamber and a liquid supply and circulation system as described in various embodiments of this application.

[0015] Compared with the prior art, the beneficial effects of the embodiments of this application are as follows: The liquid supply and circulation system for a living chamber provided in this application embodiment has a semiconductor wafer sandwiched between a first flow channel layer and a second flow channel layer that are isolated from each other. The first liquid inlet of the first flow channel layer is connected to a first liquid storage tank, and the first liquid outlet of the first flow channel layer is used to supply liquid to the living chamber. At the same time, it forms a closed loop with the heat sink. In this way, a stable and continuous working temperature difference can be formed on both sides of the semiconductor wafer, thereby improving the temperature control accuracy and stability.

[0016] The liquid supply and circulation system provided in this application embodiment enables uniform and gradual temperature changes in the circulating liquid within the first flow channel layer, achieving stable temperature control. This effectively reduces localized temperature fluctuations within the live organism chamber, preventing drastic temperature changes from adversely affecting the life state of zebrafish and other live organisms. Furthermore, the isolation between the first and second flow channel layers ensures that the circulating liquid for live organisms and the heat dissipation fluid do not interfere with or mix, improving the stability and reliability of the temperature control process. In addition, this liquid supply and circulation system reduces disturbances and impacts caused by liquid flow, effectively ensuring the stability and clarity of the optical imaging process, and meeting the requirements for long-term, high-stability, and high-resolution optical imaging of live organisms.

[0017] The above description is merely an overview of the technical solution of this application. In order to better understand the technical means of this application and to implement it in accordance with the contents of the specification, and to make the above description and other objects, features and advantages of this application more obvious and understandable, specific embodiments of this application are given below. Attached Figure Description

[0018] In drawings that are not necessarily drawn to scale, the same reference numerals may describe similar parts in different views. The same reference numerals with or without letter suffixes may indicate different instances of similar parts. The drawings illustrate various embodiments generally by way of example rather than limitation, and are used, together with the description and claims, to explain the disclosed embodiments. Where appropriate, the same reference numerals are used in all drawings to refer to the same or similar parts. Such embodiments are illustrative and not intended to be exhaustive or exclusive embodiments of the apparatus or method.

[0019] Figure 1 A schematic diagram of a liquid supply circulation system for a living body chamber according to an embodiment of this application is shown.

[0020] Figure 2 A schematic diagram showing the connection relationship of the liquid supply circulation system for a living body chamber according to an embodiment of this application is provided.

[0021] Figure 3A top view of the first flow channel layer of a liquid supply circulation system for a living chamber according to an embodiment of this application is shown.

[0022] Figure 4 A schematic diagram of the external structure of a liquid supply circulation system for a living body chamber according to an embodiment of this application is shown.

[0023] The components indicated by the reference numerals in the figure: 101-Heat exchange module; 102-Radiator; 103-First liquid storage tank; 104-Air pump; 105-Three-way valve; 106-Semiconductor chip; 107-Second liquid inlet; 108-Second liquid outlet; 109-Third liquid inlet; 110-Third liquid outlet; 111-First liquid inlet; 112-First liquid outlet; 113-Fourth liquid inlet; 114-Fourth liquid outlet; 115-Fifth liquid inlet; 116-First air inlet; 117-First air outlet; 118-Second air inlet; 119-First connection port; 120-Second liquid storage tank; 121-First centrifugal pump; 122-Second centrifugal pump; 123-Drive mechanism; 124-Fan; 125-Liquid inlet of the tank; 126-Liquid outlet of the tank; 127-Damping structure; 128-Housing shell. Detailed Implementation

[0024] To enable those skilled in the art to better understand the technical solutions of this disclosure, the disclosure will be described in detail below with reference to the accompanying drawings and specific embodiments. The embodiments of this disclosure will be further described in detail below with reference to the accompanying drawings and specific examples, but these are not intended to limit the scope of this disclosure.

[0025] The terms "first," "second," and similar words used in this disclosure do not indicate any order, quantity, or importance, but are merely used to distinguish different parts. Words such as "including" or "contains" mean that the element preceding the word encompasses the element listed after it, and do not exclude the possibility of encompassing other elements as well. Terms such as "above," "below," "left," and "right" are used only to indicate relative positional relationships; when the absolute position of the described object changes, this relative positional relationship may also change accordingly.

[0026] In this disclosure, when a specific device is described as being located between a first device and a second device, an intermediary device may or may not be present between the specific device and the first or second device. When a specific device is described as being connected to other devices, the specific device may be directly connected to the other devices without an intermediary device, or it may be not directly connected to the other devices but have an intermediary device.

[0027] All terms used in this disclosure (including technical or scientific terms) have the same meaning as understood by one of ordinary skill in the art to which this disclosure pertains, unless otherwise specifically defined. It should also be understood that terms defined in a general dictionary, such as a dictionary, should be interpreted as having a meaning consistent with their meaning in the context of the relevant art, and not as having an idealized or highly formalized meaning, unless expressly defined herein.

[0028] Techniques, methods, and equipment known to those skilled in the art may not be discussed in detail, but where appropriate, such techniques, methods, and equipment should be considered part of the specification.

[0029] This application provides a liquid supply and circulation system for a live organism chamber, wherein the live organism chamber is suitable for biomedical detection and microscopic optical imaging, and can contain zebrafish or other live organisms for real-time optical imaging observation. This liquid supply and circulation system provides a stable flow of circulating liquid to the live organisms inside the chamber, ensuring that the organisms maintain a good life state during long-term optical imaging.

[0030] The liquid can be water, physiological saline, culture medium, buffer solution or other fluids, and will not adversely affect the life state of the living organism in the live chamber, thus meeting the safety requirements of the environmental medium for live imaging experiments.

[0031] like Figure 1-2 As shown, the liquid supply and circulation system includes a first liquid storage tank 103, a heat exchange module 101, and a radiator 102. The heat exchange module 101 includes a semiconductor chip 106, a first flow channel layer, and a second flow channel layer. The semiconductor chip 106 is sandwiched between the first and second flow channel layers, which are isolated from each other. The first flow channel layer includes a first inlet 111 and a first outlet 112. The first inlet 111 communicates with the first liquid storage tank 103, allowing the circulating liquid in the first liquid storage tank 103 to flow stably into the first flow channel layer through the first inlet 111. The first outlet 112 supplies liquid to the living organism chamber. The circulating liquid, after being temperature-controlled by the heat exchange module 101, can be smoothly delivered to the living organism chamber through the first outlet 112, providing a suitable living environment for the organisms inside.

[0032] The second flow channel layer includes a second liquid inlet 107 and a second liquid outlet 108. The heat sink 102 includes a third liquid inlet 109 and a third liquid outlet 110. The third liquid inlet 109 is connected to the second liquid outlet 108, and the third liquid outlet 110 is connected to the second liquid inlet 107. This creates a temperature difference on both sides of the semiconductor chip 106, thereby ensuring that the semiconductor chip 106 regulates the temperature of the circulating liquid in the first flow channel layer. This avoids problems such as large local temperature differences caused by traditional electric heating methods and effectively improves the stability of the environment inside the live chamber.

[0033] Specifically, the first flow channel layer is used to circulate the circulating liquid supplied to the living chamber, and the second flow channel layer is used to circulate the heat dissipation fluid in the radiator 102 circuit. The two fluids do not mix or interfere with each other, which can avoid cross-contamination of fluids and ensure the stable and reliable temperature control process.

[0034] In some embodiments, the first flow channel layer and the second flow channel layer can be flat structures, which can increase the contact area between the liquid and the semiconductor wafer 106, making the liquid more uniformly heated or cooled.

[0035] In some embodiments, the first flow channel layer is located above the semiconductor wafer 106, and the second flow channel layer is located below the semiconductor wafer 106. The first flow channel layer, the semiconductor wafer 106, and the second flow channel layer are sequentially and tightly bonded together to form a sandwich structure.

[0036] Specifically, the first flow channel layer and the second flow channel layer are parallel to each other and isolated from each other. The contact surfaces of the first flow channel layer and the second flow channel layer with the semiconductor wafer 106 are flat, which can improve the heat conduction efficiency between the semiconductor wafer 106 and the two flow channels, so that heat can be transferred quickly and evenly between the two fluid layers, thereby ensuring that the temperature control process of the circulating liquid is more stable and uniform, and avoiding the problems of local overheating or temperature lag.

[0037] In some embodiments, a damping structure is provided within the first flow channel layer, the damping structure being used to maintain the flow stability of the entire circulating liquid.

[0038] The damping structure is not limited to a specific form; it can be used as long as it can reduce the impact of liquid flow.

[0039] For example, the damping structure can be a guide plate, a buffer net, a narrowing channel, or a baffle plate, or other related structures that can generate resistance to the flow of liquid.

[0040] The damping structure can guide and buffer the circulating liquid entering the first flow channel layer, effectively reducing the shaking generated during the liquid transportation process. This allows the circulating liquid to flow smoothly through the heat exchange area and be transported to the living chamber, thereby ensuring a stable environment inside the living chamber, clear imaging, and meeting the needs of long-term living optical imaging.

[0041] As a preferred embodiment, such as Figure 3 As shown, the damping structure 127 includes multiple guide plates alternately arranged along the liquid inlet direction of the first flow channel layer, and the inclination direction of each guide plate is consistent with the liquid inlet direction. The alternately arranged guide plates can gradually divert and guide the liquid entering the first flow channel layer to reduce the shaking of the liquid caused by the drive.

[0042] In some embodiments, there are multiple semiconductor wafers 106, for example, three semiconductor wafers 106 may be disposed between the first flow channel layer and the second flow channel layer.

[0043] Specifically, this application does not impose specific limitations on the dimensions of the first flow channel layer and the second flow channel layer, which can be set according to the size and number of the adapted semiconductor wafer 106.

[0044] For example, the semiconductor wafer 106 can be a rectangular wafer structure, and the length of the semiconductor wafer 106 can be set to 10mm-30mm, the width can be set to 10mm-30mm, and the thickness can be set to 1mm-5mm. The length of the first flow channel layer and the second flow channel layer can be set to 40-120mm, and the width can be set to 30-100mm, and the external dimensions of the first flow channel layer and the second flow channel layer are consistent.

[0045] In some embodiments, a plurality of semiconductor wafers 106 are arranged in an array between the first flow channel layer and the second flow channel layer, and both sides of the semiconductor wafers 106 are tightly bonded to the first flow channel layer and the second flow channel layer, respectively.

[0046] Specifically, the plurality of semiconductor wafers 106 are arranged in a matrix uniformly between the first flow channel layer and the second flow channel layer, and the semiconductor wafers 106 maintain an equal spacing, which can be 2-5 mm.

[0047] In some embodiments, the liquid supply circulation system further includes a power supply device (not shown) for supplying power to the semiconductor chip 106, which is configured to control the temperature of the liquid in the first flow channel layer based on the temperature difference.

[0048] As an example, after the power supply device applies current to the semiconductor chip 106, the charge carriers inside the semiconductor chip 106 move in a directional manner, causing the side near the first flow channel layer and the side near the second flow channel layer to form a heat absorption end and a heat release end, respectively. Simultaneously, the closed-loop circuit formed by the second flow channel layer and the heat sink 102 continuously removes heat from the heat release end through the refrigerant circulation, creating a stable and continuous temperature difference between the two sides of the semiconductor chip 106. This temperature difference is conducted to the first flow channel layer through the tightly fitting contact surfaces. Based on this temperature difference, the semiconductor chip 106 exchanges heat with the liquid in the first flow channel layer via thermal conduction, thereby achieving heating or cooling regulation of the liquid temperature in the first flow channel layer.

[0049] In some embodiments, a temperature sensor, such as an NTC thermistor or a PT10 platinum resistance thermometer, is provided on the inner or outer wall of the first flow channel layer. The temperature sensor detects the actual temperature of the liquid in the first flow channel layer in real time and feeds the temperature signal back to the controller. The controller compares the preset target temperature with the real-time temperature and dynamically adjusts the magnitude, direction, and on / off state of the current output from the power supply device to the semiconductor chip 106 based on the temperature difference, thereby adjusting the heat absorption or release efficiency of the semiconductor chip 106. In this way, the temperature of the liquid in the first flow channel layer is continuously controlled by the temperature difference, keeping it stable within the target temperature range, thereby achieving heating or cooling of the circulating liquid and improving the stability and uniformity of temperature regulation.

[0050] In some embodiments of this application, the first liquid inlet 111, the first liquid outlet 112, the second liquid inlet 107, and the second liquid outlet 108 can all adopt standardized quick-connect connectors or Luer connector structures.

[0051] In some embodiments of this application, the radiator 102 further includes a second liquid storage tank 120 and a second centrifugal pump 122. The second liquid storage tank 120 contains refrigerant, and the second centrifugal pump 122 is used to drive the refrigerant to circulate between the second liquid storage tank 120 and the second flow channel layer.

[0052] In some embodiments of this application, the radiator 102 further includes a fan 124, which is disposed on the outer wall of the second liquid storage tank 120 and is used to accelerate the heat exchange between the second liquid storage tank 120 and the external environment through forced convection.

[0053] Specifically, during the operation of the semiconductor chip 106, the heat-dissipating end near the second flow channel layer continuously releases heat. This heat is rapidly transferred to the interior of the second flow channel layer through the contact surface where the semiconductor chip 106 and the second flow channel layer are tightly bonded. The second centrifugal pump 122 continuously operates, driving the refrigerant in the second liquid storage tank 120 to enter the second flow channel layer through the third liquid outlet 110 and the second liquid inlet 107, and then returning to the second liquid storage tank 120 through the second liquid outlet 108 and the third liquid inlet 109 for circulation. During this process, the refrigerant flowing through the second flow channel layer efficiently absorbs the heat transferred to the second flow channel layer, forming a high-temperature refrigerant that is carried back to the second liquid storage tank 120. At this time, the fan 124 of the radiator 102 starts simultaneously, accelerating the heat exchange between the surface of the second liquid storage tank 120 and the external environment through forced convection, quickly dissipating the heat in the high-temperature refrigerant into the air, allowing the refrigerant to cool down and re-enter the circulation.

[0054] This ensures that the refrigerant in the second flow channel layer is always kept in a stable, lower temperature range, thereby ensuring that there is a sufficient and stable temperature difference on both sides of the semiconductor wafer 106. This provides a reliable guarantee for the precise temperature control of the circulating liquid in the first flow channel layer, effectively improving temperature control efficiency and temperature stability, and avoiding problems such as temperature control lag or excessive local temperature differences.

[0055] In some embodiments, the second centrifugal pump 122 is disposed near the second liquid storage tank 120, and the second liquid storage tank 120 and the second centrifugal pump 122 are interconnected. The second centrifugal pump 122 draws refrigerant from the second liquid storage tank 120 and pumps it to the second flow channel layer to form a heat dissipation circulation loop.

[0056] In some embodiments of this application, the refrigerant may be a chemically stable, fluid, and bubble-free thermally conductive fluid such as ethylene glycol aqueous solution, propylene glycol aqueous solution, or thermally conductive silicone oil.

[0057] In some embodiments of this application, the liquid supply circulation system further includes a three-way valve 105 and a drive mechanism 123. The three-way valve 105 includes a fifth liquid inlet 115, a second air inlet 118, and a first connection port 119. The drive mechanism 123 is used to control the three-way valve 105 to switch between the fifth liquid inlet 115 and the second air inlet 118. The fifth liquid inlet 115 is connected to the first liquid outlet 112, and the first connection port 119 is connected to the chamber liquid inlet 125 of the living chamber.

[0058] Specifically, when the liquid supply circulation system is in normal liquid supply mode, the drive mechanism 123 controls the three-way valve 105 to open the fifth inlet 115 and the first connection port 119, so that the temperature-controlled circulating liquid in the first flow channel layer is delivered to the living chamber through the first outlet, the fifth inlet 115, the first connection port 119, and the chamber body inlet 125. When it is necessary to vent, clean, or shut off the flow of the pipeline or the chamber, the drive mechanism 123 can switch the three-way valve 105 to open the second air inlet 118 and the first connection port 119. At this time, the fifth inlet 115 is closed, and air enters the living chamber through the second air inlet 118, the first connection port 119, and the chamber body inlet 125.

[0059] In some embodiments of this application, the liquid supply circulation system further includes an air pump 104, which includes a first air inlet 116 and a first air outlet 117; the first air inlet 116 is an open interface for drawing in air; the first air outlet 117 is connected to the second air inlet 118 to provide an air supply to the three-way valve 105.

[0060] In some embodiments of this application, the first air inlet 116 is provided with a filter structure to purify the intake air.

[0061] In some embodiments of this application, the air pump 104 may be a miniature diaphragm air pump, an electromagnetic air pump, or a piezoelectric miniature air pump structure.

[0062] Specifically, when the liquid supply circulation system needs to vent air, drain residual liquid, or purge the pipeline in the living chamber and pipelines, the drive mechanism 123 controls the three-way valve 105 to switch to the state where the second air inlet 118 and the first connection port 119 are connected. At this time, the air pump 104 starts working, pressurizing the air drawn in through the first air inlet 116 and then delivering it sequentially through the first air outlet 117, the second air inlet 118, and the first connection port 119 to the liquid inlet 125 of the living chamber. The air pressure is used to drain the residual liquid in the pipeline and remove any air bubbles that may be present in the pipeline, preventing air bubbles from entering the living chamber and affecting the stability of the experimental environment or causing poor fluid transport. In normal liquid supply mode, the air pump 104 is in a stopped state, the three-way valve 105 switches to the liquid passage, and the air passage where the air pump 104 is located remains closed, without interfering with the normal circulation and transport of the liquid. This achieves independent control and flexible switching between the liquid and air passages, improving the reliability and applicability of the system operation.

[0063] In some embodiments of this application, the inner diameter of the interfaces of the first inlet 111, the first outlet 112, the second inlet 107, and the second outlet 108 is 6-9 mm, the pipe diameter of the fifth inlet 115 is set to 3-4.5 mm, and the pipe diameter of the chamber inlet 125 is set to 4.7-5.5 mm. By first connecting the larger diameter first outlet 112 to the smaller diameter fifth inlet 115, and then connecting to the larger diameter chamber inlet 125, the fluctuation of buffer flow pressure can be effectively buffered, further reducing pipeline vibration and improving the stability of the liquid delivery process and the overall operational stability of the system.

[0064] In some embodiments of this application, the liquid supply circulation system further includes a first centrifugal pump 121, and the first liquid storage tank 103 includes a fourth liquid inlet 113 and a fourth liquid outlet 114, wherein the fourth liquid outlet 114 is connected to the first liquid inlet 111, the first centrifugal pump 121 is used to drive the liquid in the first liquid storage tank 103 to enter the first flow channel layer through the fourth liquid outlet 114, and the fourth liquid inlet 113 is connected to the chamber body liquid outlet 126 of the living chamber to form a liquid circulation loop in the living chamber.

[0065] In some embodiments of this application, a fourth liquid outlet 114 is provided at the bottom or side wall of the first liquid storage tank 103, and the fourth liquid outlet 114 is a standard threaded interface or a quick-connect interface.

[0066] In some embodiments of this application, the inlet end of the first centrifugal pump 121 is provided with an interface that matches the fourth outlet 114. The two are fixed by screwing together or by using a quick-connect fitting. A sealing ring (such as a fluororubber sealing ring) is fitted at the interface to ensure the connection is sealed and to prevent liquid leakage or air from entering the liquid path and generating bubbles.

[0067] Specifically, the first centrifugal pump 121 is installed below or to the side of the first liquid storage tank 103, and the height of the liquid inlet of the first centrifugal pump 121 is not higher than the height of the fourth liquid outlet 114, so as to avoid the first centrifugal pump 121 running dry or insufficient liquid intake.

[0068] The first centrifugal pump 121 and the second centrifugal pump 122 can provide a stable fluid thrust for the refrigerant, ensuring a stable circulation flow. Moreover, since the internal pressure fluctuations of the centrifugal pumps are small during operation, vibrations caused by fluid pulsation can be effectively avoided, thereby reducing the impact of fluid jitter on the stability of liquid flow in the first flow channel layer and ensuring that the imaging process is not disturbed by vibration.

[0069] Specifically, during the operation of the liquid supply circulation system, the first centrifugal pump 121 extracts the liquid stored in the first liquid storage tank 103, pressurizes it, and continuously sends it into the first flow channel layer through the fourth liquid outlet 114. The liquid exchanges heat with the semiconductor chip 106 within the first flow channel layer to achieve precise temperature regulation, and then continues to be transported to the inside of the living organism chamber through the first liquid outlet 112, the fifth liquid inlet 115, the first connection port 119, and the chamber inlet 125, providing a stable liquid environment for the living organism.

[0070] Meanwhile, the fourth inlet 113 of the first liquid storage tank 103 is connected to the liquid outlet 126 of the living chamber through a return pipeline, so that the liquid in the living chamber flows back to the first liquid storage tank 103 through the liquid outlet 126 and the fourth inlet 113, thereby forming a liquid circulation loop.

[0071] In some embodiments of this application, such as Figure 4 The liquid supply circulation system also includes a housing 128, which serves as the external protective shell for the liquid supply circulation system. An interface module is integrated on the panel of the housing 128, including a fourth liquid inlet 113 marked IN, a first connection port 119 marked OUT, and a test port TP.

[0072] The fourth liquid inlet 113 is used to connect with the liquid outlet 126 of the living chamber to realize the return of liquid to the first liquid storage tank 103. The first connection port 119 is used to connect with the liquid inlet 125 of the living chamber to transport the regulated liquid in the first flow channel layer to the living chamber.

[0073] The housing 128 not only provides enclosed physical protection, but also simplifies the pipeline connection between the system and the live animal chamber through a regular interface layout, while improving the overall structural stability of the equipment.

[0074] In some embodiments of this application, an optical imaging system is provided, which includes a living chamber and a liquid supply and circulation system as described in various embodiments of this application.

[0075] The liquid supply and circulation system forms a closed liquid circulation loop with the living organism chamber through the chamber's inlet and outlet, continuously supplying a constant-temperature liquid to the living organism chamber to provide a constant physiological environment. Simultaneously, it effectively suppresses fluid and pipeline vibrations, avoiding interference with the optical imaging process and thus ensuring the clarity and stability of image acquisition.

[0076] Furthermore, although exemplary embodiments have been described herein, their scope includes any and all embodiments based on this application that have equivalent elements, modifications, omissions, combinations (e.g., schemes involving intersections of various embodiments), adaptations, or alterations. Elements in the claims will be interpreted broadly based on the language used in the claims and are not limited to the examples described in this specification or during the implementation of this application, which will be interpreted as non-exclusive. Therefore, this specification and examples are intended to be considered illustrative only, and the true scope and spirit are indicated by the following claims and the full scope of their equivalents.

[0077] The above description is intended to be illustrative and not restrictive. For example, the above examples (or one or more of them) can be used in combination with each other. Other embodiments can be used by those skilled in the art when reading the above description. Furthermore, in the above detailed description, various features may be grouped together to simplify the application. This should not be construed as an intention that a disclosed feature not claimed is necessary for any claim. Rather, the subject matter of the application may be less than all the features of a particular disclosed embodiment. Thus, the claims are incorporated herein by reference as examples or embodiments, wherein each claim is an independent, separate embodiment, and these embodiments are contemplated as being able to be combined with each other in various combinations or arrangements. The scope of this application should be determined by reference to the appended claims and the full scope of their equivalents.

[0078] The above embodiments are merely exemplary embodiments of this application and are not intended to limit this application. The scope of protection of this application is defined by the claims. Those skilled in the art can make various modifications or equivalent substitutions to this application within its substance and scope of protection, and such modifications or equivalent substitutions should also be considered to fall within the scope of protection of this application.

Claims

1. A liquid supply and circulation system for a living body chamber, characterized in that, The liquid supply circulation system includes: First liquid storage tank; A heat exchange module includes a semiconductor wafer, a first flow channel layer, and a second flow channel layer, wherein the semiconductor wafer is sandwiched between the first and second flow channel layers, the first and second flow channel layers are isolated from each other, the first flow channel layer includes a first liquid inlet and a first liquid outlet, the first liquid inlet is connected to a first liquid storage tank, and the first liquid outlet is used to supply liquid to the living chamber; the second flow channel layer includes a second liquid inlet and a second liquid outlet; and The heat sink includes a third liquid inlet and a third liquid outlet, wherein the third liquid inlet is connected to the second liquid outlet and the third liquid outlet is connected to the second liquid inlet, thereby creating a temperature difference on both sides of the semiconductor wafer.

2. The liquid supply and circulation system according to claim 1, characterized in that, The liquid supply circulation system also includes a power supply device for supplying power to the semiconductor wafer, which is configured to control the temperature of the liquid in the first flow channel layer based on the temperature difference.

3. The liquid supply and circulation system according to claim 2, characterized in that, The semiconductor wafers are multiple, and the multiple semiconductor wafers are arranged in an array sandwiched between the first flow channel layer and the second flow channel layer, with both sides of the semiconductor wafers being tightly bonded to the first flow channel layer and the second flow channel layer, respectively.

4. The liquid supply and circulation system according to any one of claims 1-3, characterized in that, A damping structure is provided in the first flow channel layer, which is used to maintain the flow stability of the entire circulating liquid.

5. The liquid supply and circulation system according to claim 4, characterized in that, The damping structure includes multiple guide plates alternately arranged along the liquid inlet direction of the first flow channel layer, and the inclination direction of each guide plate is consistent with the liquid inlet direction.

6. The liquid supply and circulation system according to claim 1, characterized in that, The liquid supply circulation system also includes a three-way valve and a drive mechanism. The three-way valve includes a fifth liquid inlet, a second air inlet and a first connection port. The drive mechanism is used to control the three-way valve to switch between the fifth liquid inlet and the second air inlet. The fifth liquid inlet is connected to the first liquid outlet, and the first connection port is connected to the liquid inlet of the living chamber.

7. The liquid supply and circulation system according to claim 6, characterized in that, The liquid supply circulation system also includes an air pump, which includes a first air inlet and a first air outlet. The first air inlet is an open interface for drawing in air; The first air outlet is connected to the second air inlet to provide an air supply to the three-way valve.

8. The liquid supply and circulation system according to claim 1, characterized in that, The radiator also includes a second liquid storage tank and a second centrifugal pump. The second liquid storage tank contains refrigerant, and the second centrifugal pump is used to drive the refrigerant to circulate between the second liquid storage tank and the second flow channel layer.

9. The liquid supply and circulation system according to claim 1, characterized in that, The liquid supply circulation system further includes a first centrifugal pump, and the first liquid storage tank includes a fourth liquid inlet and a fourth liquid outlet, wherein the fourth liquid outlet is connected to the first liquid inlet, the first centrifugal pump is used to drive the liquid in the first liquid storage tank to enter the first flow channel layer through the fourth liquid outlet, and the fourth liquid inlet is connected to the liquid outlet of the living chamber to form a liquid circulation loop in the living chamber.

10. An optical imaging system, characterized in that, The optical imaging system includes a living chamber and a liquid supply and circulation system as described in any one of claims 1-9.

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