A biopesticide insecticidal composition containing matrine and liuyangmycin

Abstract

The present application relates to a kind of biological pesticide insecticidal composition containing matrine and liuyangmycin plant extract, and its effective component is matrine and liuyangmycin, and the ratio of the two is (1-2) :(15-30). The present application has obvious synergistic effect with the complex insecticide of matrine and liuyangmycin as effective component, and has good quick-acting and persistent effect, and the dosage of effective component is less, which reduces production cost and use cost, and is especially suitable for preventing and treating red spider mites and chinch bugs.

Description

[0001] manual Technical Field

[0002] This invention relates to the field of compound biological pesticide technology, and in particular to a biological pesticide insecticidal composition containing matrine and liuyangmycin. Background Technology

[0003] Matrine is an alkaloid extracted from the dried roots, plant, and fruit of the legume *Sophora flavescens* using organic solvents such as ethanol. It is generally classified as total matrine, and its main components include matrine, sophoridine, oxysophoridine, and sophoridine, with matrine and oxymatrine being the most abundant. Other sources include *Sophora tonkinensis* root and its aerial parts.

[0004] First, matrine is a plant-derived pesticide with specific and natural characteristics. It only acts on specific organisms and decomposes rapidly in nature, with the final products being carbon dioxide and water. Second, matrine is an endogenous plant chemical substance active against harmful organisms. Its components are not singular but a combination of multiple groups with similar chemical results and multiple groups with dissimilar chemical structures, working synergistically to exert its effect. Third, because of the combined action of multiple chemical substances, matrine is less likely to cause pesticide resistance in harmful organisms, allowing for long-term use. Fourth, it does not directly and completely kill the corresponding pests but controls their populations without seriously affecting the production and reproduction of the plant population. This mechanism is very similar to the principles of pest control in integrated pest management systems developed over decades of research after the side effects of chemical pesticides became apparent. In summary, these four points demonstrate that matrine is significantly different from general highly toxic and persistent chemical pesticides, making it a very green and environmentally friendly pesticide.

[0005] Liuyangmycin is an antibiotic with a macrocyclic lactone structure produced by *Streptomyces griseus* var. *liuyangensis*. It is a low-toxicity, low-residue, broad-spectrum acaricide effective against various mites on multiple crops. It has good contact action against spider mites on various crops, is non-systemic, and lipophilic. It is highly effective against adult and nymphal mites, but does not kill mite eggs or predatory mites. Mites are unlikely to develop resistance. It has a broad acaricidal spectrum and is effective against both spider mites and gall mites. It exhibits significant synergistic effects when combined with some organophosphate or carbamate pesticides. Its contact action against mites has a residual effect of 7–14 days.

[0006] Applying chemical pesticides is the most effective means of controlling plant pests. However, long-term, continuous, and high-dose application of chemical pesticides can easily lead to pesticide residues, environmental pollution, and resistance. The rational use of biological pesticides and their compound formulations has positive advantages such as broadening the insecticidal spectrum, improving control efficacy, extending the optimal application period, reducing dosage, minimizing phytotoxicity, reducing residues, and delaying the development of resistance. The development and research of biological pesticides can effectively solve the above problems and also address environmental pollution issues, which is of great significance to the environment and human health. Biological pesticides have received considerable attention both domestically and internationally due to their low toxicity, lack of residue, and environmental friendliness, leading to increased research and development efforts. Based on indoor screening and field trials, we have screened out a compound formulation of matrine and liuyangmycin, which exhibits a significant synergistic effect, achieving the same efficacy as chemical pesticides. Summary of the Invention

[0007] Based on the above, the purpose of this invention is to provide a novel and highly efficient biological pesticide composition. It is suitable for controlling various highly resistant pests on various crops, such as thrips, aphids, planthoppers, cabbage caterpillars, and spider mites.

[0008] The technical solution described in this invention is achieved through the following measures:

[0009] A biological pesticide insecticidal composition containing plant extracts of matrine and liuyangmycin is disclosed. The active ingredients, matrine and liuyangmycin, are combined in a binary formulation, with the remainder being auxiliary components. The mass ratio of matrine to liuyangmycin in the insecticidal composition is 0.1%–5% : 5%–20%. The insecticidal composition exhibits better control efficacy when the mass ratio is 0.3–0.8 : 8–12, and the synergistic effect is best when the mass ratio is 0.5 : 10.

[0010] The insecticidal composition of the present invention can be formulated into pesticide formulations such as emulsifiable concentrates, suspension concentrates, microemulsions, and microcapsules. The total mass of the active ingredients matrine and liuyangmycin in the formulation accounts for 5.1% to 25% of the total mass of the formulation.

[0011] The specific implementation scheme of the pesticide formulation prepared by the insecticidal composition of the present invention is as follows:

[0012] The insecticidal composition is an emulsifiable concentrate, and the components are in the following proportions by weight: matrine 0.1-5 parts; liuyangmycin 5-20 parts; conventional emulsifier 8-25 parts; conventional solvent 45-86 parts; and conventional synergist 1-5 parts. The specific production steps of this emulsifiable concentrate are as follows: first, the active ingredients matrine and liuyangmycin are completely dissolved in a solvent; then, the emulsifier and synergist are added and stirred until a homogeneous, transparent oily liquid is obtained; and finally, it is bottled to produce the emulsifiable concentrate of the composition of this invention.

[0013] The insecticidal composition is a suspension concentrate, and the components are in the following proportions by mass: matrine 0.1-5 parts; liuyangmycin 5-20 parts; dispersant 5-10 parts; antifreeze 1-5 parts; thickener 0.1-2 parts; defoamer 0.1-0.8 parts; penetrant 0-10 parts; pH adjuster 0.1-5 parts; water, balance. The specific production steps of this suspension concentrate are as follows: first, mix the other adjuvants, then mix them evenly by high-speed shearing; add the active ingredients matrine and liuyangmycin; and grind the mixture into balls in a ball mill for 2-3 hours until the particle diameter is below 5 mm, thus preparing the suspension concentrate formulation of the composition of this invention.

[0014] The insecticidal composition is a wettable powder, and the components are in the following proportions by weight: matrine 0.1-5 parts; liuyangmycin 5-20 parts; dispersant 3-10 parts; wetting agent 1-5 parts; filler, balance. The specific production steps of this wettable powder are as follows: The active ingredient matrine and liuyangmycin, along with the dispersant, wetting agent, and filler, are mixed according to the above formula, stirred evenly in a mixing tank, and then further mixed evenly using an air jet mill to produce the wettable powder of the composition of this invention.

[0015] The insecticidal composition is a microemulsion, and the components are in the following proportions by mass: matrine 0.1-5 parts; liuyangmycin 5-20 parts; emulsifier 10-30 parts; antifreeze 1-8 parts; stabilizer 0.5-10 parts; conventional solvent and co-solvent 20-50 parts; water, balance. The matrine and liuyangmycin are completely dissolved in the co-solvent, then the emulsifier, antifreeze, stabilizer, and other components are added and mixed evenly. Finally, water is added and stirred thoroughly to prepare the microemulsion.

[0016] The insecticidal composition is a microcapsule formulation, and the components are in the following mass percentages: matrine 0.1-5 parts; liuyangmycin 5-20 parts; urea 5-20 parts; formaldehyde 5-20 parts; emulsifying dispersant 5-20 parts; antifreeze 1-5 parts; thickener 0.1-2 parts; defoamer 0.1-0.8 parts; water, balance. Urea and formaldehyde (molar ratio approximately 1:1.5-2.0) are added to a three-necked flask equipped with a stirrer. The pH of the solution is adjusted to approximately 8-9 with sodium hydroxide solution, and then the temperature is raised to 70-80℃ to react and obtain a stable urea-formaldehyde resin prepolymer. A certain amount of matrine and liuyangmycin are dissolved in cyclohexane, and an emulsifying dispersant is added to the solution. With vigorous stirring, an O / W type stable emulsion is prepared with the aqueous solution containing the emulsifying dispersant as the aqueous phase. The urea-formaldehyde resin prepolymer described above is added to the emulsion, the pH value is adjusted, and a polymerization reaction occurs under acid catalysis, encapsulating the oil phase substances to form microcapsule particles. The mixture is then slowly heated and cured at 40–50°C for 1 hour. Adding appropriate additives yields a stable microcapsule formulation.

[0017] The emulsifiers mentioned above are selected from any one or a mixture of any proportion of calcium dodecylbenzenesulfonate and fatty acid polyoxyethylene ether, alkylphenol polyoxyethylene ether sulfosuccinate, styrylphenol polyoxyethylene ether, nonylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, fatty acid polyoxyethylene ester, and polyoxyethylene fatty alcohol ether.

[0018] The solvent is a mixture of one or more of the following solvents in any proportion: xylene or toluene, methanol, ethanol, n-butanol, isopropanol, rosin-based vegetable oil (code ND-60), solvent oil, dimethylformamide, dimethyl sulfoxide, and water.

[0019] The dispersant is selected from polycarboxylate codes YUS-WG5, TERSPERSE 2700, and TERSPERSE2500.

[0020] Lignosulfonates are designated as YUS-WG4, YUS-207K, WLNOK, WLNO100, TERSPERSE2020, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, alkyl sulfonate calcium salt, naphthalenesulfonic acid formaldehyde condensate sodium salt, alkylphenol polyoxyethylene ether, fatty acid polyoxyethylene ester, fatty amine polyoxyethylene ether, and glycerol fatty acid ester polyoxyethylene ether, among one or more of these.

[0021] The wetting agent is selected from one or more of the following: sodium dodecyl sulfate, calcium dodecylbenzenesulfonate, pull-opening powder BX, TERWET1004, YUS-LXC, TERSPERSE 4894, TERSPERSE2288, alkylbenzenesulfonate polyoxyethylene triphenyl phenyl phosphate, soapberry powder, silkworm excrement, and soapberry powder.

[0022] The disintegrant is selected from one or more of bentonite, urea, ammonium sulfate, aluminum chloride, citric acid, succinic acid, and sodium bicarbonate.

[0023] The thickener is selected from one or more of xanthan gum, carboxymethyl cellulose, carboxyethyl cellulose, methyl cellulose, magnesium aluminum silicate, and polyvinyl alcohol.

[0024] The stabilizer is selected from one of sodium citrate, resorcinol, epoxidized soybean oil, and epichlorohydrin.

[0025] The antifreeze is selected from ethylene glycol and glycerol.

[0026] The synergist is selected from one or more of the following: code SP-4078, organosilicon, hypertonic anesthetic, wax remover, and transdermal extract.

[0027] The defoamer is selected from one or more of silicone oil, silicone compounds, C10-20 saturated fatty acid compounds, and C8-10 fatty alcohols.

[0028] The filler is selected from one or more of kaolin, diatomaceous earth, bentonite, attapulgite, silica, starch, and light calcium carbonate.

[0029] This invention provides a compound insecticide with matrine and liuyangmycin as active ingredients, which has a significant synergistic effect, delays the development of pesticide resistance, and reduces production and usage costs. It is suitable for controlling various pests on fruit trees, vegetables, flowers and other ornamental plants, as well as field crops. Detailed Implementation

[0030] To make the technical solution, objectives, and advantages of the present invention clearer, the present invention is illustrated by the following specific embodiments, but the present invention is not limited to these examples. The effects of the present invention were tested in a field trial. Unless otherwise specified, all proportions mentioned below are by weight.

[0031] Example 1: Field efficacy trial for controlling passion fruit thrips

[0032] 10.5% Matrine·Liuyangmycin Microemulsion

[0033] Mix according to the following weight ratio:

[0034]

[0035] Experiment location: Chengmai, Hainan

[0036] Control agents: 20% acetamiprid·chlorfenapyr microemulsion, 20% imidacloprid soluble solution, 1% matrine aqueous solution, routine spraying.

[0037] Table 1: Results of field efficacy trials for controlling passion fruit thrips

[0038]

[0039] As can be seen from Table 1, Example 1 of the present invention has a significantly higher control efficacy against passion fruit thrips than other control agents, demonstrating excellent control effects. Specifically, using the same dosage, Example 1 achieved an average control efficacy of over 88% against passion fruit thrips, while other control agents only achieved 52%-75%.

[0040] Example 2: Field efficacy trial for controlling red spider mites on peppers

[0041] 10.5% Matrine·Liuyangmycin Suspension

[0042] Mix according to the following weight ratio:

[0043]

[0044]

[0045] Experiment location: Haikou, Hainan

[0046] Control agents: 5% avermectin microemulsion, 8% abamectin soluble concentrate, 20% liuyangmycin emulsifiable concentrate, conventional spraying.

[0047] Table 2: Field efficacy trials for controlling red spider mites on peppers

[0048]

[0049] As can be seen from Table 2, Example 2 of the present invention has a significantly higher control efficacy against pepper spider mites than other control agents, demonstrating excellent control effects. Specifically, using the same dosage, Example 2 achieved an average control efficacy of over 95% against pepper aphids, while other control agents only achieved 71%-75%.

[0050] Example 3: Field efficacy trial of pesticide for controlling cabbage caterpillars in Chinese cabbage

[0051] 10.5% Matrine·Liuyangmycin Microemulsion

[0052] Mix according to the following weight ratio:

[0053]

[0054] Experiment location: Chengmai, Hainan

[0055] Control agents: 20% chlorfenapyr·tebufenozide microemulsion, 20% liuyangmycin emulsifiable concentrate, 1% matrine aqueous solution, conventional spraying.

[0056] Table 3: Results of field efficacy trials for controlling cabbage caterpillars in Chinese cabbage

[0057]

[0058] As can be seen from Table 3, Example 3 of the present invention has a significantly higher control efficacy against cabbage caterpillars than other control agents, demonstrating excellent control effects. Specifically, using the same dosage, Example 3 achieved an average control efficacy of over 91.5% against cabbage caterpillars, while other control agents only achieved 75%-79%.

[0059] Example 4: Field efficacy trial for controlling bean thrips

[0060] 10.5% Matrine·Liuyangmycin Microemulsion

[0061] Mix according to the following weight ratio:

[0062]

[0063]

[0064] Experiment location: Chengmai, Hainan

[0065] Control agents: 20% acetamiprid·chlorfenapyr microemulsion, 20% liuyangmycin emulsifiable concentrate, 1% matrine aqueous solution, conventional spraying.

[0066] Table 4: Results of field efficacy trials for controlling thrips in green beans

[0067]

[0068] As can be seen from Table 4, Example 4 of the present invention has a significantly higher control efficacy against bean thrips than other control agents, demonstrating excellent control effects. Specifically, using the same dosage, Example 4 achieved an average control efficacy of over 87% against bean thrips, while other control agents only achieved 67%-75%.

[0069] Results analysis:

[0070] In summary, the insecticidal composition of this invention, containing matrine and liuyangmycin, exhibits excellent control effects against passion fruit thrips, pepper spider mites, cabbage caterpillars, and bean thrips, and is safe for the target crops. This compound formulation not only improves efficacy but also broadens the insecticidal spectrum, expands its application range, reduces costs, and provides dual control against various highly resistant pests, reducing manpower and material resources and improving production efficiency. Therefore, the invention and promotion of this compound formulation are of great social significance.

Claims

1. A biological pesticide insecticidal composition containing plant extracts of matrine and liuyangmycin, characterized in that: The mass ratio of matrine to liuyangmycin is (1-2):(15-30).

2. The insecticidal composition containing matrine and liuyangmycin according to claim 1, characterized in that: The mass ratio of matrine to liuyangmycin is 1:

20.

3. The insecticidal composition containing matrine and liuyangmycin according to claim 1, characterized in that: The insecticidal composition is formulated as an emulsifiable concentrate, suspension concentrate, microcapsule concentrate, or microemulsion.

4. The insecticidal composition containing matrine and liuyangmycin according to claim 2, characterized in that: The insecticidal composition is formulated as a microemulsion, which also contains cyclohexanone, ethanol, calcium dodecylbenzenesulfonate, styrene-based phenolic polyoxyethylene ether, fatty acid polyoxyethylene ether, and organosilicon.

5. The insecticidal composition containing matrine and liuyangmycin according to claim 3, characterized in that: The mass percentages of each component in the microemulsion are as follows: matrine 0.1–5%, liuyangmycin 5–20%, cyclohexanone 8–15%, ethanol 10–20%, calcium dodecylbenzenesulfonate 1–5%, styrene-phenol polyoxyethylene ether 15–22%, fatty acid polyoxyethylene ether 2–5%, and organosilicon 3–8%, with the balance being water.

6. The insecticidal composition containing matrine and liuyangmycin according to any one of claims 1 to 5, characterized in that: The insecticidal composition is used to kill spider mites and thrips.

Images