A brain-strengthening and intelligence-improving nutritional capsule and a preparation method thereof

Abstract

The present application relates to a kind of brain health nutrition capsule and its preparation method, belong to the technical field of functional health food.The preparation method includes the following steps: taking walnut kernel powder, DHA algal oil microcapsule powder, phosphatidylserine, ginkgo biloba extract and oligosaccharides are mixed, soft material is prepared by adding purified water, granulation is extruded by screen, and granule core is obtained by drying;Granule core is coated, and brain nutrition core granule is obtained by drying;Prepare base nutrition layer granule;Brain nutrition core granule and base nutrition layer granule are mixed, and composite granule is obtained, and composite granule is filled into capsule.The present application not only solves the three major obstacles of oxidation, precipitation and vitamin degradation of high polyphenol walnut base formula in gastric acid environment, but also unexpectedly builds from stomach protection to intestinal microecological optimization, finally realizes the cascade effect of brain nutrition efficient absorption, meets the core needs of teenagers brain health and intelligence, and truly realizes the effect of brain health and intelligence.

Description

Technical Field

[0001] This invention belongs to the technical field of functional health foods, and more specifically, relates to a brain-boosting and intelligence-enhancing nutritional capsule and its preparation method. Background Technology

[0002] With increasing global attention to brain health, especially the growing demand for brain-boosting and intelligence-enhancing products for teenagers, compound health foods with walnut kernels as the core have become a research hotspot due to their rich content of ω-3 / 6 unsaturated fatty acids, polyphenols, and plant proteins.

[0003] The current manufacturing processes for commercially available brain-boosting and intelligence-enhancing products have long been limited to physical dry mixing or wet granulation, which seriously neglects the complex interactions of various active ingredients in the gastrointestinal microenvironment, resulting in product stability and bioavailability that are significantly lower than theoretical expectations.

[0004] Although various brain-boosting formulas are available on the market, they generally suffer from compatibility issues in the gastrointestinal microenvironment. For example, when high-polyphenol walnut powder coexists with DHA algal oil, in a simulated gastric juice environment (pH 1.2-2.0, 37℃), the catechol structure in the walnut polyphenols is easily oxidized under acidic conditions to generate quinone free radicals, which catalyze a chain oxidation reaction of the unsaturated double bonds of DHA, producing a rancid odor and losing neuroprotective activity. When high-polyphenol walnut powder coexists with ginkgo biloba extract, the terpene lactones (ginkgolides A / B) in the ginkgo biloba extract form insoluble complexes with walnut protein at low pH through hydrophobic interactions and hydrogen bonds, resulting in a high in vitro precipitation rate. This not only reduces the content of active ingredients but may also cause gastrointestinal discomfort in users.

[0005] To address the aforementioned issues, existing technologies have attempted the following improvement approaches, but these have certain limitations. For example, while individual microencapsulation or enteric coating of a single ingredient can prevent the oxidation of DHA in the acidic environment of the stomach to some extent, it fails to address the interactions between other components. Whole-form enteric coating, while avoiding damage to individual components from stomach acid, may lead to delayed release of water-soluble components such as zinc and vitamin B in the intestines, affecting the actual efficacy of the product. Some existing technologies attempt to solve the problem by adding exogenous antioxidants (such as vitamin E) or chelating agents, but the introduction of non-claimed ingredients can easily raise safety concerns.

[0006] In summary, the compatibility issues of existing brain-boosting formulas in the acidic environment of the stomach have not been effectively resolved, and there is a lack of technological inspiration to achieve synergistic effects between the gut and brain axis through process innovation. Therefore, there is an urgent need in this field for a brain-boosting nutritional composition that can break through conventional understanding to meet the actual needs of adolescents for brain health and cognitive enhancement. Summary of the Invention

[0007] The purpose of this invention is to provide a brain-boosting and intelligence-enhancing nutritional capsule and its preparation method, which has the characteristics of increasing the production of short-chain fatty acids in the intestine and promoting the expression of MCT1 transporter protein in the blood-brain barrier.

[0008] The objective of this invention can be achieved through the following technical solutions: A method for preparing a brain-boosting and intelligence-enhancing nutritional capsule includes the following steps: S1. Take walnut kernel powder, DHA algal oil microcapsule powder, phosphatidylserine, ginkgo leaf extract and oligogalactose and mix them in a weight ratio of (30-40):(10-15):(2-3):(3-5):(4-8). Add 25-35% purified water to make soft material, extrude it through a sieve and dry it to obtain the granule core. S2. A coating solution is prepared using an acrylic resin aqueous dispersion. The particle cores obtained in step S1 are coated in a fluidized bed and dried to obtain brain nutrition core particles. S3. Mix vitamin B complex, zinc gluconate, β-carotene, sucralose, isomaltooligosaccharide, silicon dioxide and maltodextrin, and then dry granulate and granulate to obtain basic nutrient layer granules. S4. Mix the brain nutrient core particles obtained in step S2 with the basic nutrient layer particles obtained in step S3 at a weight ratio of (6.5-7.5):(2.5-3.5), and spray them with a 5-15% oligofructose aqueous solution in a fluidized bed at 35-45℃ to obtain composite particles. Control the dry weight of oligofructose to account for 2-4% of the total weight of the composite particles, and dry them until the moisture content is ≤2%. S5. Fill the capsule with the composite particles obtained in step S4, fill with nitrogen and seal to obtain the finished product.

[0009] Furthermore, before step S1, the walnut kernel powder is pretreated, including the following steps: take the walnut kernel powder, freeze it at -15℃ to -25℃ for 10-20 minutes, and then perform ultra-fine grinding to control the particle size ≤30 micrometers.

[0010] Among them, freezing pretreatment helps to form nanoscale ice crystals in walnut cell walls, selectively embrittles cellulose-rich cell walls rather than destroying lipid bodies. After pulverization, the exposure density of phenolic hydroxyl groups of walnut polyphenols (mainly ellagic acid and gallic acid) increases and the exposure is more uniform. The exposed phenolic hydroxyl groups and the subsequently added oligogalactose form a three-dimensional hydrogen bond network during granulation. After this network is embedded in the enteric coating membrane, the water vapor permeability of the membrane layer is reduced, thereby improving the density and absorption and utilization rate of the coating membrane.

[0011] Further, in step S1, walnut kernel powder, DHA algal oil microcapsule powder, phosphatidylserine, ginkgo leaf extract and oligogalactose are mixed in a weight ratio of (34-36):(12-14):(2-3):(3-5):(5-7).

[0012] Further, in step S1, the aperture of the screen is 0.5-2mm; the drying in step S1 refers to drying at 45-55℃ until the moisture content is ≤6%.

[0013] In this process, walnut polyphenols and ginkgo extracts exhibit a vicious cycle of oxidation and precipitation in the gastric acid environment: the oxidation intermediates of walnut polyphenols accelerate the degradation of ginkgo terpene lactones, and the degradation products further promote the denaturation of walnut proteins. In step S1, walnut kernel powder, DHA algal oil microcapsule powder, phosphatidylserine (PS), ginkgo leaf extract, and galactooligosaccharides are mixed and granulated. Galactooligosaccharides act as a "molecular bridge," with their galactose units forming hydrogen bonds with walnut polyphenols and their glucose units generating π-π stacking with ginkgo flavonoids, anchoring the four active ingredients inside the granule core and blocking the vicious cycle in gastric acid at its source.

[0014] Further, the specific preparation steps of step S2 are as follows: the acrylic resin aqueous dispersion and purified water are mixed at a mass ratio of 1:0.8-1.2 to obtain a coating solution. Then, the particle cores are put into a fluidized bed coating machine, preheated to 35-45℃, and the coating solution is sprayed onto the particle cores by spraying to perform coating.

[0015] Further, in step S2, the solid content of the food-grade acrylic resin aqueous dispersion is 29-31%, and it is diluted with purified water to a solid content of 10-20%; the coating weight gain is monitored by real-time weighing, and spraying is terminated when the coating weight gain is 18-22% of the particle core weight.

[0016] Among these factors, the aforementioned coating weight gain ratio is more conducive to the stability of the coating effect. When the coating weight gain is <18%, the membrane layer is prone to nanoscale pores, leading to failure of gastric juice protection; when the coating weight gain is >22%, the membrane layer is prone to excessive density, leading to obstruction of intestinal release.

[0017] Furthermore, the drying described in step S2 refers to drying at 50-60°C until the moisture content is ≤3%.

[0018] Further, in step S3, based on a total of 100 parts by weight, the vitamin B complex is 0.3-1 parts, the zinc gluconate is 0.2-0.5 parts, β-carotene is 0.05-0.2 parts, sucralose is 0.05-0.2 parts, isomaltooligosaccharide is 30-50 parts, silicon dioxide is 0.3-1 parts, and the balance is maltodextrin.

[0019] More preferably, in step S3, based on a total of 100 parts by weight, the vitamin B complex is 0.4-0.6 parts, the zinc gluconate is 0.2-0.3 parts, β-carotene is 0.08-0.12 parts, sucralose is 0.05-0.15 parts, isomaltooligosaccharide is 30-40 parts, silicon dioxide is 0.4-0.6 parts, and the balance is maltodextrin.

[0020] The zinc gluconate contains 10-30% zinc. Zinc is an important component of many enzymes, participating in DNA synthesis and neurotransmitter function, and is especially important for children and adolescents during their growth and development. The B vitamins (VB1), including B1, B6, B12, and folic acid, work together to help the body convert food into energy, maintain normal nervous system function, and are crucial for brain energy metabolism. Beta-carotene has antioxidant properties and is converted into vitamin A in the body as needed, which is beneficial for vision and brain health.

[0021] In step S3, the order of preparation of the basic nutrient layer is not important.

[0022] Furthermore, based on a total of 100 parts by weight, the basic nutrient layer particles also contain 0.05-0.2 parts of prebiotics.

[0023] Further, the mixing weight ratio of the brain nutrient core particles and the basic nutrient layer particles in step S4 is (6.8-7.2):(2.8-3.2).

[0024] The mixed basal nutrient layer particles and brain nutrient core particles are coated with an aqueous solution of oligofructose and dried to form a thin film. The above-mentioned mixing ratio can prevent zinc ions in the basal layer from penetrating the coating film and causing oxidation. When the mixing ratio is <6.5, zinc ions in the basal nutrient layer can easily penetrate the coating film defects in gastric juice and catalyze DHA oxidation; when the mixing ratio is >7.5, the concentration of vitamin B complex released is lower than the effective threshold.

[0025] Walnut polyphenols and ginkgo flavonoids, acting as prebiotic synergists, work synergistically with the basic nutrient layer to form a gradient prebiotic system, increasing the production of short-chain fatty acids in the gut. Short-chain fatty acids have been shown to upregulate the expression of the blood-brain barrier MCT1 transporter protein, enhancing blood-brain barrier permeability, thereby optimizing the gut microenvironment and promoting brain nutrient absorption.

[0026] Further, after step S4 and before step S5, the obtained composite particles are subjected to sterilization treatment. Preferably, the sterilization treatment is irradiation sterilization or low-temperature sterilization.

[0027] Furthermore, in step S5, the capsule is a 00# plant-based hard capsule.

[0028] By preparing it into hard capsules, the composite particles can be completely contained, the enteric structure can be protected, and the filling process is simple and low-cost.

[0029] Furthermore, the walnut kernel powder is from Lvliang, Shanxi.

[0030] As a national geographical indication product production area, the walnut kernels from Lüliang, Shanxi Province, have been tested and found to have a polyphenol content of ≥3.5% (calculated as gallic acid) and a linoleic acid content of over 60%, making them widely used in high-end brain health products.

[0031] A brain-boosting and intelligence-enhancing nutritional capsule is prepared using the above method.

[0032] The aforementioned brain-boosting and intelligence-enhancing nutritional capsules are used in the preparation of health food products that improve memory and learning abilities in adolescents.

[0033] Furthermore, the adolescents are aged 12-18 years, and the dosage is 1-2 capsules orally daily, each capsule containing 500mg ± 10% of the total weight, for ≥28 consecutive days. It is preferably recommended to take it after breakfast or lunch.

[0034] The beneficial effects of this invention are: (1) This invention solves the three major obstacles of oxidation, precipitation and vitamin degradation of high polyphenol walnut-based formula in gastric acid environment through freezing pretreatment, controlling the coating weight gain ratio, reasonable component layering strategy and systematic process design. It also unexpectedly constructs a cascade effect from gastric protection to intestinal microecology optimization, and finally achieves efficient absorption of brain nutrition, so as to meet the core needs of adolescents to improve brain health and intelligence and truly achieve the effect of improving brain health and intelligence.

[0035] (2) This invention provides a solid foundation of nutrients (such as protein, fat, and vitamin E) and a variety of active ingredients (such as antioxidant peptides and polyphenols) through walnut kernel powder, while PS, DHA algal oil microcapsule powder, and ginkgo leaf extract enhance specific brain functional pathways (such as neurotransmitter metabolism, cell membrane fluidity, and cerebral blood flow). The polyphenols and vitamin E in walnuts, together with the flavonol glycosides and β-carotene in ginkgo leaf extract, form a powerful antioxidant network, reducing oxidative stress and inflammatory responses in brain cells and providing a healthy internal environment for the brain. B vitamins and zinc, as key coenzyme factors, participate in the brain's energy metabolism and the synthesis and regulation of neurotransmitters, ensuring efficient transmission of nerve signals. Detailed Implementation

[0036] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described below with reference to specific embodiments, but the scope of protection of this invention is not limited thereto. Experimental methods not specifically described in the embodiments are generally performed under conventional conditions or according to the manufacturer's recommendations. Unless otherwise specified, all reagents and materials used are commercially available.

[0037] Raw material description: Shanxi Lvliang walnut kernel powder: HPLC analysis showed a polyphenol content of 3.82±0.15% (gallic acid equivalent), and GC analysis showed a linoleic acid content of 62.3±0.15%. It was passed through an 80-mesh sieve for later use. DHA algal oil microcapsule powder: DHA content 15.2±0.15%, passed through a 100-mesh sieve for later use; Ginkgo biloba leaf extract: HPLC analysis showed total flavonoids of 24.7±0.15% and terpene lactones of 6.3±0.15%; Acrylic resin aqueous dispersion: Evonik Eudragit® L30D-55, food grade, solids content 30.2±0.15%; Zinc gluconate: Zinc content is approximately 14.2%.

[0038] A method for preparing a brain-boosting and intelligence-enhancing nutritional capsule includes the following steps: S1. Pre-treatment of walnut kernel powder by freezing: Weigh 350g of walnut kernel powder and spread it evenly on a 304 stainless steel tray (thickness ≤0.8cm); place it in a precision freezer and freeze at -20±0.5℃ for 12±0.5 minutes; then transfer it to an air jet mill with a feeding speed of 4.8kg / h and a grinding pressure of 0.68MPa, and grind it under nitrogen protection; use a laser particle size analyzer to monitor in real time and collect ultrafine powder with D90=24.7±1.2μm, and keep the ambient temperature ≤25℃ throughout the process.

[0039] S2. Granule Core Preparation: 120g of DHA microcapsule powder, 25g of phosphatidylserine, 40g of ginkgo extract, 60g of galactooligosaccharides, and 350g of pretreated walnut kernel powder were added to a mixer and mixed at 15 rpm for 20 min. Then, 178±2g of purified water (approximately 30% of the total powder weight) was slowly sprayed in, and mixing continued for 8 min until a soft material was formed. The mixture was then extruded through a gyratory granulator and granulated using a 1.0mm stainless steel screen. Finally, the mixture was dried in a fluidized bed dryer at 50±1℃ for 28 min, and the moisture content was measured to be ≤5.8%, yielding the granule core.

[0040] S3. Coating of Brain Nutrition Core Particles: Mix 348g of acrylic resin L30D-55 aqueous dispersion and 348g of purified water, and stir magnetically for 30min to obtain a 15.1% coating solution; put the particle cores into a fluidized bed coating machine and preheat to a material temperature of 40±0.5℃; then spray, controlling the coating parameters as follows: inlet air temperature 55±1.0℃, material temperature 40±0.5℃, spray pressure 0.3MPa, and peristaltic pump spray speed 8±0.2g / min; pause spraying and weigh every 5min, and stop spraying when the weight gain reaches 20.3±0.2% of the particle core weight; continue drying for 15min until the moisture content is ≤2.7%, to obtain brain nutrition core particles.

[0041] S4. Preparation of basic nutrient layer granules: Weigh 1.5g of vitamin B complex, 0.7g of zinc gluconate, 0.3g of β-carotene, 0.3g of sucralose, 90g of isomaltooligosaccharide, 1.5g of silicon dioxide, and 205.7g of maltodextrin, place them in a mixer, and mix at 20 rpm for 25 min; then, use a dry granulator to make thin sheets under a roller pressure of 4±0.1MPa; then, granulate them through a 20-mesh sieve using a granulator, and dry them until the moisture content is ≤2.3% to obtain basic nutrient layer granules.

[0042] S5. Preparation of composite particles: 630±1g of brain nutrition core particles and 300±1g of basic nutrition layer particles (mass ratio of approximately 7:3) were mixed at a mixing speed of 10 rpm for 15 min. Then, the fluidized bed was preheated to 40±0.5℃ and sprayed with 300g of a 10% concentration of fructooligosaccharide solution at a spraying rate of 5g / min. When the dry weight of fructooligosaccharide accounted for 3% of the total weight of the composite particles, the spraying was stopped. Finally, the particles were dried until the moisture content was ≤1.8% to obtain the composite particles.

[0043] S6. Capsule filling and packaging: 00# plant capsules are filled using an automatic capsule filling machine, with a filling amount of 500±5.0mg / capsule; then nitrogen filling and sealing are performed, followed by aluminum foil barrier packaging to obtain the finished product.

[0044] Example 2 Compared with Example 1, the difference in this example is that in step S1, the freezing conditions are changed to: temperature control -15.0±0.5℃ freezing for 10±0.5 min; in step S2, the DHA microcapsule powder is 120g, phosphatidylserine is 30g, ginkgo extract is 50g, and galactooligosaccharides are 70g. The remaining components, preparation steps, and parameters are the same.

[0045] Example 3 Compared to Example 1, the difference in this example is that in step S2, the amount of DHA microcapsule powder is 120g, phosphatidylserine is 20g, ginkgo extract is 30g, and galactooligosaccharides is 50g. In step S3, spraying is terminated when the weight gain reaches 19±0.2% of the particle core weight. The remaining components, preparation steps, and parameters are the same.

[0046] Example 4 Compared with Example 1, the difference in this example is that in step S4 of this example, the amount of basic nutrient layer particles used is as follows: vitamin B complex is 1.8g, zinc gluconate is 0.8g, β-carotene is 0.25g, sucralose is 0.45g, isomaltooligosaccharide is 110g, silicon dioxide is 2g, and maltodextrin is 184.7g.

[0047] Comparative Example 1 Compared with Example 1, the difference in this comparative example lies in the preparation method of the nutritional capsules: 350g of walnut kernel powder, 120g of DHA microcapsule powder, 25g of phosphatidylserine, 40g of ginkgo extract, 60g of galactooligosaccharides, 8g of vitamin B complex, 7g of zinc gluconate, 3g of β-carotene, 0.5g of sucralose, 45g of isomaltooligosaccharide, 2g of silicon dioxide, and 234.5g of maltodextrin were directly weighed and placed in a mixer, and mixed at 20 rpm for 35 minutes. Then, the mixture was granulated into thin sheets using a dry granulator under a roller pressure of 4±0.1 MPa. The granules were then granulated through a 20-mesh sieve using a granulator and dried until the moisture content was ≤2.3%, yielding composite granules. Finally, 00# plant capsules were filled using an automatic capsule filling machine, with a filling weight of 500±5.0 mg / capsule. The capsules were then nitrogen-sealed and packaged with aluminum foil barrier to obtain the finished product.

[0048] Comparative Example 2 Compared to Example 1, this comparative example differs in that, in step S2, only 120g of DHA microcapsule powder and 350g of pretreated walnut kernel powder are mixed to prepare the granule core, which is then coated in step S3 to obtain brain nutrition core granules. In step S5, the brain nutrition core granules, basic nutrient layer granules, 25g of phosphatidylserine, 40g of ginkgo extract, and 60g of galactooligosaccharides are mixed to obtain composite granules. All other components, preparation steps, and parameters remain the same.

[0049] Comparative Example 3 Compared with Example 1, this comparative example differs in that the basic nutrient layer particles in step S4 are prepared in advance. These particles are then directly added to step S2 along with the pretreated walnut kernel powder and placed in a mixer. The mixture is stirred at 20 rpm for 35 minutes, followed by slow spraying of 268±2 g of purified water (approximately 30% of the total powder weight), and mixing continues for 10 minutes until a soft mass is formed. The mixture is then extruded using a gyratory granulator and granulated through a 1.5 mm stainless steel screen. Finally, it is dried in a fluidized bed dryer at 50±1℃ for 32±2 minutes, with a moisture content ≤5.8%, yielding granular cores. Steps S3 and S6 are then performed, while step S5 is omitted. All other components, preparation steps, and parameters remain the same.

[0050] The samples obtained in Examples 1-4 and Comparative Examples 1-3 were subjected to the following performance tests: (1) In vitro stability and release rate test Take 16.4 mL of dilute hydrochloric acid and 10 g of pepsin, add water to 1000 mL, adjust the pH to 1.2 ± 0.1, and prepare artificial gastric fluid; take 6.8 g of disodium hydrogen phosphate, 3 g of potassium dihydrogen phosphate, and 10 g of pancreatin, add water to 1000 mL, adjust the pH to 6.8 ± 0.1, and prepare artificial intestinal fluid.

[0051] Take 6 capsules from the same batch, aseptically cut open the capsule shells, collect the contents, mix well, weigh 526±1mg of the contents, and determine the DHA content to be approximately 10mg using gas chromatography according to GB5009.168.

[0052] Gastric juice test: The contents particles were directly added to 100 mL of artificial gastric juice preheated to 37±0.5℃ and stirred at 50 rpm.

[0053] DHA loss rate determination: Following GB5009.168, after 2 hours of gastric juice treatment, the entire system (gastric juice + particles + precipitate) was transferred to a separatory funnel, and 20 mL of n-hexane:isopropanol (3:2) was added. Extraction was performed three times consecutively. The organic phases were then combined, dried under nitrogen, and methylated before GC analysis (internal standard: methyl heptadecanoate). The DHA loss rate was calculated as follows: DHA loss rate = [(initial DHA amount - DHA amount after gastric juice treatment) / initial DHA amount] × 100%.

[0054] DHA precipitation rate determination: The centrifugal weighing method was used. After gastric fluid treatment for 2 hours, the sample was centrifuged at 3000 rpm for 10 minutes. The precipitate was then dried at 105℃ to constant weight, and the precipitation rate was determined. The precipitation rate was calculated as follows: (dry weight of precipitate / total solids of sample) × 100%.

[0055] Intestinal fluid test: After treating the contents of Examples 1-4 and Comparative Examples 2 and 3 with gastric fluid for 2 hours, the particles were removed with a filter, gently rinsed 3 times with 0.9% NaCl solution, and then transferred to 100 mL of artificial intestinal fluid (37±0.5℃). Stirring was started at 50 rpm, and t=0 was the instant of transfer to the intestinal fluid. The contents of Comparative Example 1 were directly added to 100 mL of artificial intestinal fluid (37±0.5℃), and stirring was started at t=0.

[0056] DHA release rate determination: 10 mL of sample was taken after 30 min and filtered through a 0.45 μm filter membrane. The DHA release rate of the filtrate was measured according to GB5009.168. The DHA release rate was calculated as follows: (Amount of DHA released from intestinal fluid over 30 min / Initial amount of DHA) × 100%.

[0057] Determination of vitamin B1 release rate: Referring to GB5009.279, another 10 mL sample was taken from the intestinal fluid after 15 min, and the vitamin B1 content of the intestinal fluid after 15 min was determined by fluorescence spectrophotometry.

[0058] (2) Animal brain function improvement experiment Animals and Grouping: Eighty SPF-grade SD rats (4 weeks old, male, 80±10g) were randomly divided into 8 groups (n=10). These were the blank control group, Examples 1-4 groups, and Comparative Examples 1-3 groups. The blank control group was directly administered 0.5% sodium carboxymethyl cellulose solution (0.1g / mL) by gavage. Examples 1-4 groups used the product from Examples 1-4, and Comparative Examples 1-3 groups used the sample from Comparative Examples 1-3. The contents of the capsules in each group were accurately weighed after conversion to the human equivalent dose and suspended in 0.5% sodium carboxymethyl cellulose solution (0.1g / mL). The suspension was ultrasonically dispersed for 5 minutes to ensure uniformity. This was followed by continuous gavage for 30 days. The Morris water maze test was performed on days 26-30.

[0059] Water maze setup: A circular pool, 120±2cm in diameter and 50cm in height; water depth 30±1cm; water temperature 22±0.5℃ (constant temperature circulation system); platform 10cm in diameter and 28cm in height (2cm below the water surface). Four fixed visual references (triangle / circle / stripes / grid) were placed around the pool; the laboratory lighting was uniform. For the three days prior to the test, rats were allowed to swim freely in the pool for 60 seconds daily (without the platform) to eliminate novelty stress.

[0060] Day 26 execution: The platform was placed 2cm above the water surface in the southwest quadrant; each rat was tested twice (15min interval), and the time to find the platform within 60s was recorded.

[0061] From day 27 to 30, at fixed times each day, the rats were trained at the water entry point 4 times. The water entry point was rotated in the order of east, south, west, and north (to avoid path habits). The rats were gently placed facing the pool wall and the timer was started. If the rats found the platform within 60 seconds, they stayed for 15 seconds. If they did not find the platform, they were guided to stay on the platform for 15 seconds. The latency period was recorded as 60 seconds. The interval between each training session was ≥15 minutes (resting in the cage).

[0062] On day 31, the platform was removed, and the rats entered the water from the opposite (southeast) quadrant of the original platform and swam freely for 60 seconds. The number of times the rats crossed the original platform position within 60 seconds after the platform was removed was recorded.

[0063] The test results are shown in Table 1.

[0064] Table 1 As shown in Table 1, Examples 1-4 were significantly superior to Comparative Examples 1-3 in terms of gastric protection, intestinal release, and improvement of brain function. Comparative Example 3 demonstrated that while the full-formula enteric coating could achieve gastric protection, its poor intestinal release index resulted in a severe delay in the release of water-soluble nutrients (VB1 release rate). In contrast, Examples 1-4 of this application, through a layered design with an enteric coating on the brain nutrient core and no enteric coating on the basal nutrient layer, achieved rapid VB1 release while maintaining gastric protection, effectively balancing the conflict between protection and release. Specifically, the escape latency of Examples 1-4 was significantly lower than that of Comparative Examples 1-3 and the blank control group; the number of times Examples 1-4 crossed the original platform position was much higher than that of the blank control group and all comparative examples, indicating that the samples of this application have an improving effect on memory function.

[0065] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present invention. Any simple modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. A method for preparing a brain-boosting and intelligence-enhancing nutritional capsule, characterized in that, A method for preparing a brain-boosting and intelligence-enhancing nutritional capsule includes the following steps: S1. Take walnut kernel powder, DHA algal oil microcapsule powder, phosphatidylserine, ginkgo leaf extract and oligogalactose and mix them in a weight ratio of (30-40):(10-15):(2-3):(3-5):(4-8). Add 25-35% purified water to make soft material, extrude it through a sieve and dry it to obtain the granule core. S2. A coating solution is prepared using an acrylic resin aqueous dispersion. The particle cores obtained in step S1 are coated in a fluidized bed and dried to obtain brain nutrition core particles. S3. Mix vitamin B complex, zinc gluconate, β-carotene, sucralose, isomaltooligosaccharide, silicon dioxide and maltodextrin, and then dry granulate and granulate to obtain basic nutrient layer granules. S4. Mix the brain nutrient core particles obtained in step S2 with the basic nutrient layer particles obtained in step S3 at a weight ratio of (6.5-7.5):(2.5-3.5), and spray them with a 5-15% oligofructose aqueous solution in a fluidized bed at 35-45℃ to obtain composite particles. Control the dry weight of oligofructose to account for 2-4% of the total weight of the composite particles, and dry them until the moisture content is ≤2%. S5. Fill the capsule with the composite particles obtained in step S4, fill with nitrogen and seal to obtain the finished product.

2. The method for preparing a brain-boosting and intelligence-enhancing nutritional capsule according to claim 1, characterized in that, Before step S1, the walnut kernel powder is pretreated, including the following steps: take the walnut kernel powder, freeze it at -15℃ to -25℃ for 10-20 minutes, and then perform ultra-fine grinding to control the particle size ≤30 micrometers.

3. The method for preparing a brain-boosting and intelligence-enhancing nutritional capsule according to claim 1, characterized in that, In step S1, the aperture of the screen is 0.5-2mm; the drying in step S1 refers to drying at 45-55℃ until the moisture content is ≤6%.

4. The method for preparing a brain-boosting and intelligence-enhancing nutritional capsule according to claim 1, characterized in that, The specific preparation steps of step S2 are as follows: the acrylic resin aqueous dispersion and purified water are mixed at a mass ratio of 1:0.8-1.2 to obtain a coating solution. Then, the particle cores are put into a fluidized bed coating machine, preheated to 35-45℃, and the coating solution is sprayed onto the particle cores by spraying to perform coating.

5. A method for preparing a brain-boosting and intelligence-enhancing nutritional capsule according to claim 1 or 4, characterized in that, In step S2, the solid content of the food-grade acrylic resin aqueous dispersion is 29-31%, and it is diluted with purified water to a solid content of 10-20%. The coating weight gain is monitored by real-time weighing, and spraying is terminated when the coating weight gain is 18-22% of the particle core weight. The drying process described in step S2 refers to drying at 50-60℃ until the moisture content is ≤3%.

6. The method for preparing a brain-boosting and intelligence-enhancing nutritional capsule according to claim 1, characterized in that, In step S3, based on a total weight of 100 parts, the vitamin B complex is 0.3-1 part, zinc gluconate is 0.2-0.5 parts, β-carotene is 0.05-0.2 parts, sucralose is 0.05-0.2 parts, isomaltooligosaccharide is 30-50 parts, silicon dioxide is 0.3-1 part, and the balance is maltodextrin.

7. The method for preparing a brain-boosting and intelligence-enhancing nutritional capsule according to claim 1, characterized in that, The mixing weight ratio of brain nutrient core particles to basic nutrient layer particles in step S4 is (6.8-7.2):(2.8-3.2).

8. The method for preparing a brain-boosting and intelligence-enhancing nutritional capsule according to claim 1, characterized in that, The walnut kernel powder mentioned is from Lvliang, Shanxi.

9. A brain-boosting and intelligence-enhancing nutritional capsule, characterized in that, It is prepared by the preparation method described in any one of claims 1-8.

10. The use of the brain-boosting and intelligence-enhancing nutritional capsule as described in claim 9 in the preparation of health food products that improve the memory and learning ability of adolescents.

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