A hair care composition for synergistically fixing and repairing hair color with quaternary ammonium salt-80 and a preparation method thereof

Abstract

The present application belongs to the field of hair dyeing post-fixing and damage repairing, discloses a kind of glycoprotein and quaternary ammonium salt-80 synergistic fixing and repairing hair care composition and its preparation method, the composition is composed of plant source glycoprotein, quaternary ammonium salt-80, transglutaminase, acryl glycoprotein conjugate and PBS buffer solution;In the preparation process, glycoprotein and quaternary ammonium salt-80 are blended and adsorbed under mild conditions, then enzymatically catalyzed crosslinking, and homogenized by ultrasonic assistance, without organic solvent, green and environmentally friendly process;Acryl glycoprotein conjugate is prepared by coupling acryloyl chloride with glycoprotein hydroxyl group, which can further crosslink under the action of light and heat;The composition uses multi-layer biological membrane and enzymatic covalent network to realize long-lasting color fixing and micro-damage repair of dyed hair.

Description

Technical Field

[0001] This invention belongs to the field of hair color fixing and damage repair after dyeing, specifically relating to a shampoos and conditioners composition for synergistic color fixing and repair by glycoprotein and quaternary ammonium salt-80 and its preparation method. Background Technology

[0002] As people's demands for hair care continue to rise, hair dyeing has become an important part of daily hair care. However, dyed hair is prone to fading, dryness, and cuticle damage, which directly affects the user experience and the durability of the dyeing effect. Existing shampoo and conditioner compositions mainly use cationic surfactants, silane-modified polymers, or protein derivatives to form a physical coating film on the hair surface to achieve a "color-locking" effect. However, these film-forming agents are easily detached by the mechanical action of washing, causing dye molecules to be carried away by the water flow, resulting in a still relatively high fading rate. At the same time, the simple film layer has limited effect on deep repair of micro-fractures and cuticle cracking inside the hair fibers, and cannot effectively restore the smoothness and shine of the hair.

[0003] To enhance repair performance, some products add high-molecular-weight proteins or silicone oils to their formulations, attempting to improve cuticle damage by filling or covering it. However, these formulations typically rely on high-temperature processing, organic solvent media, or chemical modification processes, which not only increase production costs but may also damage protein structures, reduce bioactivity, and pose environmental pollution and product safety risks. Furthermore, traditional protein formulations often fail to achieve good synergy with surfactants, film-forming agents, and other ingredients, easily leading to mutual precipitation or instability, further limiting their overall performance in color fixation and deep repair. Summary of the Invention

[0004] To address the shortcomings mentioned in the background art, the present invention aims to provide a shampoos and conditioners composition for synergistic color fixation and repair of glycoprotein and quaternary ammonium salt-80, and its preparation method. Through low-temperature mixing, enzymatic cross-linking and functional coupling techniques, a multi-layer composite network is constructed to achieve efficient and long-lasting color fixation, deep micro-damage repair and self-healing functions for dyed hair.

[0005] The objective of this invention can be achieved through the following technical solutions: A shampoos and conditioners composition for synergistic color-fixing and repair by glycoprotein and quaternary ammonium salt-80, comprising the following raw materials in parts by weight: 1.0-3.0 parts of plant-derived glycoprotein, 0.5-1.5 parts of quaternary ammonium salt-80, 0.1-0.5 parts of transglutaminase, 0.2-0.8 parts of acryloyl glycoprotein conjugate, and 0.5-10 parts of excipients; The excipients consist of one or more of the following: water-soluble thickeners, preservatives, humectants, conditioning agents, fragrances, and pH adjusters.

[0006] More preferably, the plant-derived glycoprotein is hydrolyzed soybean protein or hydrolyzed sugarcane protein with a molecular weight of 10-30 kDa.

[0007] More preferably, the preparation method of the acryloyl glycoprotein conjugate specifically includes the following steps: S101. Weigh the plant-derived glycoprotein powder and PBS buffer, add them to a magnetic stirrer and stir to prepare a homogeneous glycoprotein solution; S102. Acryloyl chloride and triethylamine are added sequentially to the glycoprotein solution and stirred to mix, so that the acryloyl group is chemically coupled to the glycoprotein molecule to form a reaction mixture; S103. Remove unreacted small molecule reagents and impurities by dialysis bags or organic solvent precipitation, collect the purified product and freeze-dry it to obtain a white powdery acryloyl glycoprotein conjugate.

[0008] More preferably, the transglutaminase is a microbial transglutaminase with an activity of 50-200 U / g.

[0009] More preferably, after 10 washes with neutral water, the hair fading rate is ≤20%.

[0010] More preferably, the acryloyl glycoprotein conjugate has photocrosslinkable groups and can form a crosslinked network after ultraviolet light irradiation to enhance wash resistance.

[0011] A method for preparing a shampoos and conditioners composition that synergistically fixes and repairs color with glycoprotein and quaternary ammonium salt-80 includes the following steps: S1. Weigh the plant-derived glycoprotein powder and quaternary ammonium salt-80, add them separately to PBS buffer, add excipients, and stir magnetically until completely dissolved to obtain the solutions of each raw material; S2. Combine the solutions obtained in step S1, continue stirring, then add acryloyl glycoprotein conjugate powder and stir until uniformly dispersed; S3. Add transglutaminase dispersion to the above mixture, stir gently for a certain period of time, then place it in an ultrasonic homogenizer for treatment, and adjust the pH to 6.5-7.0 with acid-base solution; S4. Remove undissolved particles and microorganisms through a 0.45 μm filter, collect the clarified liquid and fill it to obtain the shampoos and conditioners composition with synergistic color-fixing and repairing effects of the glycoprotein and quaternary ammonium salt-80.

[0012] More preferably, the ultrasonic homogenization power in step S3 is 150–300 W, and the treatment time is 3–7 minutes.

[0013] More preferably, the obtained composition is irradiated with ultraviolet light before step S4 to activate the photocrosslinking groups on the acryloyl glycoprotein conjugate and form a further crosslinked protective film.

[0014] The beneficial effects of this invention are: This invention successfully constructs a multilayer composite network structure by achieving synergistic adsorption of natural glycoproteins and quaternary ammonium salt-80 under mild conditions, and by introducing transglutaminase-catalyzed covalent crosslinking and photo- and thermo-responsive crosslinking technology of acryloyl glycoprotein conjugates. This structure forms a firmly adhered biocompatible protective film on the surface of dyed hair. This protective film not only effectively locks in dye molecules, preventing them from being detached during repeated washing due to mechanical shearing or water rinsing, thus significantly reducing the fading rate, but also fills the gaps in the hair cuticle under enzymatic covalent crosslinking, restoring the integrity of the fiber's internal structure and improving the hair's softness and shine. Simultaneously, the photocrosslinkable groups of the conjugates further crosslink into a network under light or heat conditions, endowing the film with excellent wash resistance and self-repairing functions. When slight abrasion occurs on the hair surface, the remaining crosslinked groups and functional microcapsule system can continuously release active ingredients, rapidly repairing microscopic cracks and achieving continuous self-healing. Furthermore, the preparation process of this invention does not require the use of organic solvents and high-temperature treatment. It only involves mixing, enzymatic catalysis, and ultrasonic homogenization within the range of room temperature to medium temperature, which greatly reduces energy consumption and environmental pollution risks, thus meeting the development needs of green cosmetics. Attached Figure Description

[0015] The invention will now be further described with reference to the accompanying drawings.

[0016] Figure 1 Bar charts showing the comparison of color fixation rate before and after crosslinking of hair treated with the shampoo and conditioner compositions of Examples 1-3 and Comparative Examples 1-2; Figure 2 Line graphs showing the color fixation rate versus number of washes after hair was treated with the shampoo and conditioner compositions of Examples 1-3 and Comparative Examples 1-2. Detailed Implementation

[0017] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0018] Example 1 I. Preparation of Acryloyl Glycoprotein Conjugates Weigh 2.0 g of plant-derived glycoprotein powder and place it in 100 mL of PBS buffer (pH 7.0). Stir magnetically at room temperature for 30 min until completely dissolved to obtain a glycoprotein solution. Then place the solution in an ice bath, maintaining the reaction temperature at 0–5 °C, and continue stirring. Dissolve 0.35 mL of triethylamine in 10 mL of dichloromethane (DCM) and 1.2 mL of acryloyl chloride in 5 mL of DCM to prepare triethylamine and acryloyl chloride solutions, respectively. Under ice bath and high-speed stirring, first slowly add the triethylamine solution to capture the hydrogen chloride produced in the reaction, then add the acryloyl chloride solution dropwise over 30 min. After the addition is complete, continue stirring at 0–5 °C for 2 h.

[0019] After the reaction was complete, an equal volume of ice-cold anhydrous ethanol was added to terminate the coupling reaction and precipitate the protein. The mixture was allowed to stand for 10 min to allow low-molecular-weight impurities to precipitate, and the supernatant was discarded. The precipitate was redissolved in a small amount of PBS, placed in a dialysis bag with a molecular weight cutoff of 3.5 kDa, and dialyzed at 4 °C with deionized water for 48 h, changing the water every 6 h until no free acryloyl chloride and triethylamine remained in the dialysate. The dialyzed protein solution was aliquoted into lyophilized bottles, pre-frozen to –40 °C, and then freeze-dried under vacuum for 24 h until the dried product contained <1% water, yielding the acryloyl glycoprotein conjugate.

[0020] Preparation of a shampoos and conditioners composition for synergistic color-fixing and repair by glycoproteins and quaternary ammonium salt-80 The shampoo and conditioner composition comprises the following raw materials in parts by weight: 1.0 part of plant-derived glycoprotein, 0.5 part of quaternary ammonium salt-80, 0.1 part of transglutaminase, 0.2 part of acryloyl glycoprotein conjugate, and 0.5 part of excipients; The preparation steps are as follows: 80 mL of PBS (pH 7.0) was placed in a 250 mL glass beaker and magnetically stirred at 500 rpm at room temperature. 1.0 g of glycoprotein powder was slowly added, and stirring continued for 30 min until completely dissolved. Then, 0.5 g of quaternary ammonium salt-80 and 0.5 g of excipients were added sequentially, and stirring continued for 20 min. 0.2 g of acryloyl glycoprotein conjugate powder was slowly added to the above mixture, and stirring was continued at 500 rpm for 15 min to ensure the conjugate was fully dispersed and adsorbed onto the glycoprotein medium. The reaction temperature was raised to 37 °C, and 0.1 g of transglutaminase was added. The reaction was maintained at 37 °C and stirred at 300 rpm for 45 min. The enzymatically reacted mixture was then irradiated under a 365 nm UV lamp for approximately 10 min, and then transferred to an ultrasonic homogenizer. The mixture was treated at 200 W power for 5 min in intermittent mode (5 s on / 3 s off) to break down the aggregates. After the reaction is complete, allow the system to cool naturally to room temperature, adjust the pH to 6.5 with 0.1 M hydrochloric acid or sodium hydroxide solution, and then filter through a 0.45 μm microporous membrane to remove undissolved particles and microorganisms. Collect the clear liquid, which is the shampoos and conditioners composition for synergistic color fixing and repair of glycoprotein and quaternary ammonium salt-80.

[0021] Example 2 The preparation of the acryloyl glycoprotein conjugate was the same as in Example 1.

[0022] The preparation method of the shampoos and conditioners composition for synergistic color-fixing and repair by glycoprotein and quaternary ammonium salt-80 is as follows: The shampoo and conditioner composition comprises the following raw materials in parts by weight: 3.0 parts of plant-derived glycoprotein, 1.5 parts of quaternary ammonium salt-80, 0.5 parts of transglutaminase, 0.8 parts of acryloyl glycoprotein conjugate, and 10 parts of excipients; The preparation steps of the shampoos and conditioners composition for synergistic color-fixing and repair by glycoprotein and quaternary ammonium salt-80 are the same as in Example 1.

[0023] Example 3 The preparation of the acryloyl glycoprotein conjugate was the same as in Example 1.

[0024] The preparation method of the shampoos and conditioners composition for synergistic color-fixing and repair by glycoprotein and quaternary ammonium salt-80 is as follows: The shampoo and conditioner composition comprises the following raw materials in parts by weight: 2.0 parts of plant-derived glycoprotein, 1.0 part of quaternary ammonium salt-80, 0.3 parts of transglutaminase, 0.5 parts of acryloyl glycoprotein conjugate, and 5.25 parts of excipients; The preparation steps of the shampoos and conditioners composition for synergistic color-fixing and repair by glycoprotein and quaternary ammonium salt-80 are the same as in Example 1.

[0025] Comparative Example 1 (without transglutaminase) The preparation of the acryloyl glycoprotein conjugate was the same as in Example 1.

[0026] The preparation method of the shampoos and conditioners composition for synergistic color-fixing and repair by glycoprotein and quaternary ammonium salt-80 is as follows: The shampoo and conditioner composition comprises the following raw materials in parts by weight: 2.0 parts of plant-derived glycoprotein, 1.0 part of quaternary ammonium salt-80, 0.5 parts of acryloyl glycoprotein conjugate, and 5.25 parts of excipients; The preparation steps are as follows: 80 mL of PBS was placed in a 250 mL beaker and magnetically stirred at 500 rpm at room temperature. 2.0 g of glycoprotein powder was slowly added and stirred for 30 min. While continuously stirring, 1.0 g of quaternary ammonium salt-80 and 5.25 g of excipients were added sequentially, and stirring was continued for 20 min. Then, 0.5 g of acryloyl glycoprotein conjugate powder was slowly added, and stirring was maintained at 500 rpm for 15 min to ensure complete dispersion. The mixture was irradiated under a 365 nm UV lamp for about 10 min, then transferred to an ultrasonic homogenizer and treated with 200 W power intermittently (5 s on / 3 s off) for 5 min to break down microaggregates. After cooling to room temperature, the pH of the system was adjusted to 6.5 with 0.1 M hydrochloric acid or sodium hydroxide solution. The mixture was filtered through a 0.45 μm microporous membrane to remove undissolved particles and microorganisms. The clear liquid was collected, which is the glycoprotein and quaternary ammonium salt-80 synergistic color-fixing and repairing washing and conditioning composition.

[0027] Comparative Example 2 (without acryloyl glycoprotein conjugate) The preparation method of the shampoos and conditioners composition for synergistic color-fixing and repairing effects of protein and quaternary ammonium salt-80 is as follows: The shampoo and conditioner composition comprises the following raw materials in parts by weight: 2.0 parts of plant-derived glycoprotein, 1.0 part of quaternary ammonium salt-80, 0.3 parts of transglutaminase, and 5.25 parts of excipients; The preparation steps were as follows: 80 mL of PBS was added to a 250 mL beaker and magnetically stirred at 500 rpm at room temperature. 2.0 g of glycoprotein powder was slowly added and stirred for 30 min. Under stirring conditions, 1.0 g of quaternary ammonium salt-80 and 5.25 g of excipients were added sequentially, and stirring was continued for 20 min to ensure complete dissolution and uniform dispersion. The solution was heated to 37 °C, and 0.3 g of transglutaminase was added under stirring at 300 rpm. The reaction was allowed to proceed for 45 min. After the reaction, the mixture was irradiated under a 365 nm UV lamp for about 10 min, then transferred to an ultrasonic homogenizer and treated with 200 W power for 5 min (intermittent: 5 s on / 3 s off) to break up aggregates and improve homogeneity. The mixture is cooled to room temperature, and the pH is adjusted to 6.5 using 0.1 M hydrochloric acid or sodium hydroxide solution. It is then filtered through a 0.45 μm microporous membrane to remove undissolved particles and microorganisms. The clear liquid is collected, which is the shampoos and conditioners composition for synergistic color fixing and repair of glycoprotein and quaternary ammonium salt-80.

[0028] Performance testing 1. Post-dyeing washing color fixation rate test Dyed human hair strands, approximately 2–3 cm in diameter and 10 cm in length, were immersed in the shampoo and conditioner compositions of Examples 1–3 and Comparative Examples 1–2 of this invention. The mixtures were gently stirred for 5 minutes in a 25 °C water bath at a concentration of 1 g / L. After removing the samples and shaking off excess liquid, they were allowed to air dry. The dried samples were numbered, and their reflectance (R0) was initially measured using a spectrophotometer (λ = 560 nm). Subsequently, each sample was placed in neutral water and gently washed for 1 minute at 100 rpm, then spun dry and air dried. This wash-dry process was repeated 10 times. The reflectance (R0) was measured after the nth drying cycle. n The fixation rate is calculated using the following formula:

[0029] Rwater white represents the baseline reflectance value of undyed hair, and the results are shown in Table 1 below.

[0030] Table 1 Fading rate and color fixation rate

[0031] As shown in Table 1, the embodiments of the present invention maintained a significantly higher color-fixing effect and lower fading rate than the comparative examples even after multiple washes, fully verifying the synergistic mechanism of glycoprotein and quaternary ammonium salt-80, the combined effect of enzymatic cross-linking, and the acryloyl coupling compound. Although Example 1 was a low-ratio formulation, the introduction of enzymatic covalent cross-linking resulted in an initial color-fixing rate of 80% for the dye in the film layer. Example 2, by increasing the ratio, further enhanced the surface adsorption and cross-linking network of both components, increasing the color-fixing rate to 82%. Example 3, using the optimal intermediate ratio, not only achieved the best balance between glycoprotein film formation and cation adsorption but also utilized the deep network formed by enzymatic cross-linking and the photo / thermal cross-linking groups of the coupling compound, achieving a maximum color-fixing rate of 85% and a fading rate of only 15%. In contrast, the color-fixing rates of Comparative Examples 1 and 2 decreased to 60% and 62%, respectively, after the removal of the key enzyme or coupling compound, further confirming the crucial role of enzymatic cross-linking and functional coupling compounds in elution resistance and long-lasting color-fixing.

[0032] 2. Self-healing performance test Dyed human hair bundles (2–3 cm in diameter, 10 cm in length) that had undergone 10 washes with neutral water were grouped, and artificial slits approximately 20 μm wide were made on the hair surface using a fine needle. Each group of samples was immersed in an environmental chamber at 37 °C and 80% relative humidity for 4 hours to simulate the self-healing process under normal humid and hot conditions. After the healing process, the slit area was photographed using a scanning electron microscope at the same magnification, and the slit width was measured (W0 is the initial slit width, W4 is the width after 4 hours). The self-healing recovery rate was calculated using the following formula:

[0033] The results are shown in Table 2 below.

[0034] Table 2 Recovery Rate Results

[0035] As shown in Table 2, the embodiments of the present invention exhibit significantly greater sustained self-repair capabilities compared to the comparative samples, fully verifying the synergistic effect of glycoprotein and quaternary ammonium salt-80, enzymatic cross-linking, and the multi-composite network design of acryloyl conjugates on the repair of micro-damage. Example 1 achieved 50% closure of the crack width with a simple formulation, demonstrating that basic enzymatic cross-linking can promote the formation of a semi-solidified structure of the biomembrane at the crack site. Example 2, by increasing the proportion of active ingredients in the formulation, achieved a hair repair rate of 55%, indicating that the synergistic adsorption and thickening of the membrane layer by quaternary ammonium salt-80 and glycoproteins enhances the filling efficiency of hair micro-cracks. Example 3, using a moderate formulation, achieved the best results, reducing the hair crack width from 20 μm to 7 μm, corresponding to a 65% recovery rate, demonstrating the crucial role of the deep cross-linked network constructed by the conjugates after photo / thermal cross-linking in self-healing performance. In contrast, the self-healing rates of Comparative Example 1 (without transglutaminase) and Comparative Example 2 (without acryloyl conjugate) were only 25% and 30%, respectively, indicating that the lack of enzymatic covalent crosslinking or functional conjugates would significantly weaken the "self-adhesion" and repair capabilities of the membrane.

[0036] 3. Washing resistance test Samples of the shampoo and conditioner compositions from Examples 1–3 and Comparative Examples 1–2 were taken respectively. Dyed human hair strands (2–3 cm in diameter, 10 cm in length) were immersed in a 1 g / L formulation solution, stirred at 25 °C and 500 rpm for 5 min, then spun dry and air-dried. Each sample was divided into two groups: "non-photocrosslinked" and "photocrosslinked." The photocrosslinked group was irradiated with a 365 nm UV lamp at 25 °C for 10 min to activate the acryloyl coupling compound; the non-crosslinked group received no light treatment. Both groups were then subjected to a "wash-dry" cycle 10 times. After the 10th drying cycle, the reflectance R was measured. 10 , and unstained background R 100 And by comparing the initial reflectance R0, the fixation rate is calculated:

[0037] The results are shown in Table 3 below.

[0038] Table 3 Fixation Rate Results

[0039] As shown in Table 3, the photocrosslinking function of the acryloyl glycoprotein conjugate significantly enhances the wash-resistance of the washing and conditioning composition. Without photocrosslinking treatment, the fixation rates of Examples 1–3 were 70%, 80%, and 85%, respectively, which were already significantly improved compared to the comparative samples. After 10 minutes of irradiation with 365 nm ultraviolet light, the fixation rates of Examples 1–3 further increased to 78%, 88%, and 92%, especially Example 3, which increased from 85% to 92%, demonstrating the strong "locking" effect of the multilayer crosslinking network on dye molecules. In contrast, Comparative Examples 1 and 2 only increased from 60% to 62% and from 62% to 64%, respectively, with increases not exceeding 4 percentage points, indicating that formulations lacking functional conjugates cannot form a stable photocrosslinked layer even under light irradiation. This invention introduces a photocrosslinkable conjugate into the basic synergistic system of glycoprotein-quaternary ammonium salt-80, and uses ultraviolet light to induce the generation of an additional covalent network structure. This not only strengthens the binding force between the film layer and the fiber, but also significantly reduces the shedding of dye molecules during repeated washing, thereby achieving a long-lasting color-fixing effect that surpasses traditional washing and care products.

[0040] Formulation stability and compatibility testing The shampoo and conditioner compositions of Examples 1–3 and Comparative Examples 1–2 were aliquoted into samples, with 30 mL of each sample placed in three constant temperature incubators and stored in the dark at 4 °C, 25 °C, and 40 °C for 1, 3, and 6 months, respectively. At each time point, after sampling, clarity was first determined visually and by transmitted light (600 nm spectrophotometer) to observe for any visible or microscopic precipitation. Next, the pH change (ΔpH) was measured using a pH meter. Then, the viscosity change (ΔmPa·s) was measured using a rotational viscometer at 25 °C and 100 rpm. All measurements were performed in triplicate and compared with the initial values. If a sample showed significant turbidity or precipitation under any condition, and the pH or viscosity change exceeded ±0.5 units or ±10%, the formulation was considered unstable. The results are shown in Table 4 below.

[0041] Table 4. Results of Formulation Stability and Compatibility

[0042] As shown in Table 4, after long-term observation for 1, 3, and 6 months under three storage conditions (4 °C, 25 °C, and 40 °C), Examples 1–3 showed almost no pH shift (ΔpH only 0.1), slight viscosity changes (Δviscosity 5–6%), and clarity remained within a very low fluctuation range (≤2%), with no visible or microscopic precipitation. This result fully demonstrates that the formulation of this invention, under a mild and green preparation process, successfully inhibits component stratification, aggregation, or degradation through the synergistic stable adsorption of glycoproteins and quaternary ammonium salt-80, as well as the cross-linking network formed by enzymatic cross-linking and acryloyl couplings, ensuring high stability and compatibility between formulation ratios and the overall formulation.

[0043] 5. Hair mechanical strength test Human hair bundles (2–3 mm in diameter, 50 mm in length) were selected after dyeing and 10 washes. They were treated with the products of Examples 1–3 and Comparative Examples 1–2 and then air-dried. A miniature tensile testing machine was used with a clamping spacing of 30 mm and a tensile rate of 10 mm / min to test the breaking strength and elongation at break (elongation at break / initial clamping length × 100%) of each hair bundle. Each sample was repeated 5 times, and the average value and standard deviation were calculated. The results are shown in Table 5 below.

[0044] Table 5 Hair Mechanical Strength

[0045] As shown in Table 5, the embodiments of the present invention significantly enhance the mechanical strength and elongation properties of dyed hair. Example 1 increases the breaking strength to 1.20 N and the elongation to 15.0%, which are approximately 33% and 25% higher than the comparative samples (0.90 N, 12.0%), respectively. Example 2 increases the content of glycoprotein and quaternary ammonium salt-80, increasing the breaking strength to 1.35 N and the elongation to 16.5%, exhibiting stronger intrinsic fiber toughness. Example 3 uses an intermediate ratio and combines a dual cross-linking mechanism of acryloyl glycoprotein conjugate and enzyme-catalyzed cross-linking, increasing the breaking strength to 1.50 N and the elongation to 18.0%, which is more than 50% higher than the comparative samples 1-2. This indicates that the secondary cross-linking of the photocrosslinkable conjugate on the enzyme-catalyzed network not only thickens the film layer but also forms additional cross-linking points inside the fiber to disperse tensile stress. The comparative samples, lacking an enzyme-catalyzed covalent network or functional conjugate, have inferior performance.

[0046] In the description of this specification, references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the invention. In this specification, illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0047] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed invention.

Claims

1. A shampoos and conditioners composition for synergistic color-fixing and repairing effects of glycoprotein and quaternary ammonium salt-80, characterized in that, It contains the following raw materials in parts by weight: 1.0-3.0 parts of plant-derived glycoprotein, 0.5-1.5 parts of quaternary ammonium salt-80, 0.1-0.5 parts of transglutaminase, 0.2-0.8 parts of acryloyl glycoprotein conjugate, and 0.5-10 parts of excipients; The excipients consist of one or more of the following: water-soluble thickeners, preservatives, humectants, conditioning agents, fragrances, and pH adjusters.

2. The shampoos and conditioners composition for synergistic color-fixing and repairing of glycoprotein and quaternary ammonium salt-80 according to claim 1, characterized in that, The plant-derived glycoprotein is hydrolyzed soybean protein or hydrolyzed sugarcane protein with a molecular weight of 10-30 kDa.

3. The shampoos and conditioners composition for synergistic color-fixing and repairing of glycoprotein and quaternary ammonium salt-80 according to claim 1, characterized in that, The preparation method of the acryloyl glycoprotein conjugate specifically includes the following steps: S101. Weigh the plant-derived glycoprotein powder and PBS buffer, add them to a magnetic stirrer and stir to prepare a homogeneous glycoprotein solution; S102. Acryloyl chloride and triethylamine are added sequentially to the glycoprotein solution and stirred to mix, so that the acryloyl group is chemically coupled to the glycoprotein molecule to form a reaction mixture; S103. Remove unreacted small molecule reagents and impurities by dialysis bags or organic solvent precipitation, collect the purified product and freeze-dry it to obtain a white powdery acryloyl glycoprotein conjugate.

4. The shampoos and conditioners composition for synergistic color-fixing and repairing of glycoprotein and quaternary ammonium salt-80 according to claim 1, characterized in that, The transglutaminase is a microbial transglutaminase with an activity of 50–200 U / g.

5. The shampoos and conditioners composition for synergistic color-fixing and repairing of glycoprotein and quaternary ammonium salt-80 according to claim 1, characterized in that, After 10 washes with neutral water, the hair color fading rate is ≤20%.

6. The shampoos and conditioners composition for synergistic color-fixing and repairing of glycoprotein and quaternary ammonium salt-80 according to claim 1, characterized in that, The acryloyl glycoprotein conjugate has photocrosslinkable groups and can form a crosslinked network after ultraviolet light irradiation to enhance wash resistance.

7. A method for preparing a shampoos and conditioners composition for synergistic color-fixing and repair by glycoprotein and quaternary ammonium salt-80, wherein the shampoos and conditioners composition for synergistic color-fixing and repair by glycoprotein and quaternary ammonium salt-80 is as described in any one of claims 1-6, characterized in that, Includes the following steps: S1. Weigh the plant-derived glycoprotein powder and quaternary ammonium salt-80, add them separately to PBS buffer, add excipients, and stir magnetically until completely dissolved to obtain the solutions of each raw material; S2. Combine the solutions obtained in step S1, continue stirring, then add acryloyl glycoprotein conjugate powder and stir until uniformly dispersed; S3. Add transglutaminase dispersion to the above mixture, stir gently for a certain period of time, then place it in an ultrasonic homogenizer for treatment, and adjust the pH to 6.5-7.0 with acid-base solution; S4. Remove undissolved particles and microorganisms through a 0.45 μm filter, collect the clarified liquid and fill it to obtain the shampoos and conditioners composition with synergistic color-fixing and repairing effects of the glycoprotein and quaternary ammonium salt-80.

8. The method for preparing the shampoos and conditioners composition for synergistic color-fixing and repairing by glycoprotein and quaternary ammonium salt-80 according to claim 7, characterized in that, The ultrasonic homogenization power in step S3 is 150-300 W, and the treatment time is 3-7 minutes.

9. The method for preparing the shampoos and conditioners composition for synergistic color fixing and repair of glycoprotein and quaternary ammonium salt-80 according to claim 7, characterized in that, Prior to step S4, the obtained composition is irradiated with ultraviolet light to activate the photocrosslinking groups on the acryloyl glycoprotein conjugate, forming a protective film for further crosslinking.

Images