Extract of jujube and its extraction method and application

Abstract

This invention belongs to the field of plant extraction technology, specifically relating to Alpinia oxyphylla extract, its extraction method, and applications. The extraction method involves homogenizing and crushing the raw material, adding specific enzymes, activated carbon, and diatomaceous earth, and utilizing an integrated cell wall breaking and impurity removal process. Enzymes release essential oils while activated carbon adsorbs impurities and pigments. Diatomaceous earth not only aids filtration but also shortens subsequent freeze-drying time and the purification process. After purification, a filter cake is obtained through pressure filtration. This filter cake is then subjected to freeze-drying and supercritical CO2 extraction to obtain the Alpinia oxyphylla extract. The above extraction method is simple to operate, has low production costs, and a shorter total production time. Furthermore, this method can significantly improve the yield of Alpinia oxyphylla extract, and the entire extraction process is conducted at low temperatures without the use of organic solvents, reducing the volatilization of essential oils in the extract and ensuring no solvent residue in the essential oil, resulting in a high-quality essential oil product that can be applied in cosmetics, food, health products, and other fields.

Description

Technical Field

[0001] This invention belongs to the field of plant extraction technology, specifically relating to Alpinia oxyphylla extract, its extraction method, and its application. Background Technology

[0002] Plant volatile oils, also known as plant essential oils, are secondary metabolites produced by plants that have a fragrant aroma. Due to their diverse biological activities, they are widely used in the food, pharmaceutical, and cosmetic industries.

[0003] Alpinia oxyphylla is a perennial tropical herb belonging to the genus Alpinia in the ginger family (Zingiberaceae). It is one of the four major southern Chinese medicinal herbs and is listed by the Ministry of Health as a food and medicine homology product. Alpinia oxyphylla essential oil is the main active ingredient of Alpinia oxyphylla. There are various extraction methods, such as steam distillation, solvent extraction, ultrasound-assisted extraction, and supercritical CO2 extraction. Alpinia oxyphylla essential oil contains over 100 components, and the resulting components and their activities vary considerably depending on the extraction method used.

[0004] Chinese patent application CN115141682A discloses a method for extracting essential oil from Alpinia oxyphylla. This method utilizes repeated exposure to low-temperature ultrasound and freezing to disrupt the cell walls of the raw material, followed by traditional steam distillation to separate the essential oil. Although this method enhances the release of essential oil through pretreatment, some low-boiling-point volatile components may be lost during distillation. Furthermore, high temperatures may cause the decomposition of a small amount of heat-sensitive components, affecting the quality of the essential oil.

[0005] Chinese patent application CN117959398A discloses a method for extracting volatile oil from Alpinia oxyphylla using supercritical CO2 extraction. The method involves directly drying and pulverizing the Alpinia oxyphylla raw material before it enters a supercritical extraction device for extraction. However, this method lacks a raw material pretreatment step, which prevents the effective release of essential oil components, affecting the composition of the essential oil and the extraction rate, resulting in insufficient utilization of raw materials.

[0006] Therefore, it is of great significance to develop an extraction method for Alpinia extract that fully preserves essential oil components and natural activity. Summary of the Invention

[0007] This invention addresses the shortcomings of existing methods for extracting Alpinia oxyphylla extract, with the primary objective of providing a method for extracting Alpinia oxyphylla extract.

[0008] A second objective of this invention is to provide an Alpinia oxyphylla extract prepared by the extraction method described above.

[0009] A third objective of this invention is to provide applications of the said brain-boosting extract.

[0010] The above-mentioned objective of this invention is achieved through the following technical solution: This invention protects a method for extracting an extract that enhances cognitive function, comprising the following steps: S1. Mix Alpinia oxyphylla fruit or kernel with a buffer solution with pH 5-6, homogenize and crush, then add compound enzyme and activated carbon, and fully enzymatically hydrolyze and decolorize to obtain the initial extract. S2. Add diatomaceous earth to the crude extract obtained in step S1, stir thoroughly, filter under pressure, take the filter cake and freeze dry to obtain dried material; S3. The dried material obtained in step S2 is subjected to supercritical CO2 extraction to obtain Alpinia extract; The complex enzyme is selected from two or more of papain, cellulase, pectinase, hemicellulase, glucanase, β-glucosidase, and protease.

[0011] Preferably, the amount of the compound enzyme added is 1.0wt% to 2.0wt% of fresh Alpinia oxyphylla fruit or Alpinia oxyphylla kernel.

[0012] Furthermore, the complex enzyme is selected from any of the following combinations: a combination of papain (first enzyme) and cellulase (second enzyme), a combination of cellulase (first enzyme) and pectinase (second enzyme), or a combination of β-glucanase (first enzyme) and β-glucosidase (second enzyme).

[0013] Preferably, in the above combination, the mass ratio of the first enzyme and the second enzyme in the complex enzyme is 1:(1~3), more preferably 1:(1~2).

[0014] Preferably, the complex enzyme is a combination of papain and cellulase.

[0015] Furthermore, the extraction method includes one or both of the following (1) to (2): (1) The amount of activated carbon added is 1wt% to 3wt% of Alpinia oxyphylla fruit or Alpinia oxyphylla kernel; (2) The amount of diatomaceous earth added is 2.0%wt to 5.0%wt of Alpinia oxyphylla fruit or Alpinia oxyphylla kernel.

[0016] Furthermore, the stirring is carried out for 1 to 3 hours, preferably 1.5 to 2.5 hours.

[0017] Furthermore, the freeze-drying step is as follows: first, freeze at -45~-40℃ for 2~3 hours, and then dry at -7~5℃ and 10~15Pa for 2~3 hours.

[0018] Furthermore, the pressure at which freezing occurs at -45 to -40°C is atmospheric pressure.

[0019] Furthermore, the parameters of the supercritical CO2 extraction include one or more of the following (1) to (4): (1) The extraction pressure is 10~15MPa, and the extraction temperature is 40~50℃; (2) The CO2 flow rate is 10~20m³ / h. 3 / h, extraction time is 2~3h; (3) primary separation pressure is 6~10MPa, separation temperature is 45~55℃; (4) secondary separation pressure is 5~7MPa, separation temperature is 45~55℃.

[0020] Preferably, the activated carbon has a D 90 The thickness is 60~90μm, more preferably 70~80μm.

[0021] Preferably, the diatomaceous earth has a D 90 The thickness is 60~80μm, more preferably 65~75μm.

[0022] Preferably, the temperature for complete enzymatic hydrolysis and decolorization is 35~40℃.

[0023] Furthermore, the buffer solution comprises at least one of citrate-disodium hydrogen phosphate, acetic acid-sodium acetate, and citrate-sodium hydroxide.

[0024] As an optional implementation, the brain-boosting fruit is a fresh brain-boosting fruit.

[0025] Furthermore, the fresh cardamom fruit refers to the original fruit that has just been harvested, has not been dried or processed, and retains a high level of moisture and natural flavor.

[0026] Furthermore, the fresh cardamom fruit undergoes pretreatment before use, which includes washing and crushing.

[0027] This invention also protects the Alpinia oxyphylla extract prepared by the extraction method.

[0028] This invention also protects the use of the Alpinia oxyphylla extract prepared by the extraction method in the preparation of one or more cosmetics, foods, and health products.

[0029] Furthermore, the cosmetic is a cosmetic with skin repair and / or soothing effects.

[0030] Furthermore, the cosmetics can be prepared into dosage forms such as ointments, creams, lotions, and liquids.

[0031] Compared with the prior art, the present invention has the following beneficial effects: (1) The extraction method of the present invention involves homogenizing and crushing the raw materials, adding specific enzymes, activated carbon and diatomaceous earth, and utilizing the integrated cell wall breaking and impurity removal. While the enzymes release essential oils, the activated carbon adsorbs impurities and pigments. The diatomaceous earth not only aids filtration but also shortens the subsequent freeze-drying time and purification process. After purification, the filter cake is obtained by pressure filtration. The obtained filter cake is then freeze-dried and supercritical CO2 extracted in sequence to obtain the Alpinia oxyphylla extract.

[0032] (2) The extraction method is simple to operate, has low production cost, and shorter total production time.

[0033] (3) The extraction method can significantly improve the yield of Alpinia oxyphylla extract.

[0034] (4) The extraction process is carried out at low temperature and without the use of organic solvents, which reduces the volatilization of essential oils in the extract and ensures that there are no solvent residues in the essential oils, resulting in high-quality essential oil products. Attached Figure Description

[0035] Figure 1 This is a statistical graph showing the caudal fin area of ​​zebrafish in the sample group (i.e., the Alpinia extract obtained in Example 1), the model group, and the normal control group. ***: Compared with the model group, P< 0.001. Detailed Implementation

[0036] The present invention will be further described below with reference to the accompanying drawings and specific embodiments, but the embodiments do not limit the present invention in any way. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in this technical field.

[0037] Unless otherwise specified, all reagents and materials used in the following examples are commercially available.

[0038] Activated carbon, purchased from Guangdong Tongke Activated Carbon Co., Ltd., product model TKTS-21, D 90 It is 75 μm.

[0039] Diatomaceous earth, purchased from Longzexin Technology (Shenzhen) Co., Ltd., product model D3010, D 90 It is 69.5 μm.

[0040] Quartz sand, purchased from Gongyi Deco Water Purification Materials Co., Ltd., custom-made, D 90 It is 0.15mm.

[0041] Papain, cellulase, pectinase, β-glucanase, β-glucosidase, and bromelain were all purchased from Ningxia Xiasheng Enzyme Technology Co., Ltd.

[0042] Example 1 This embodiment provides a method for extracting an extract that enhances intelligence, the method comprising the following steps: (1) Take 1 kg of freshly cleaned Alpinia oxyphylla fruit, crush it and add 1 L of citrate-disodium hydrogen phosphate buffer solution (pH 5.5), homogenize for 2 min, add 5 g of papain, 10 g of cellulase and 20 g of activated carbon, stir and react for 2 h at 40 °C to obtain the initial extract.

[0043] (2) Add 30g of diatomaceous earth to the initial extract obtained in step (1), stir for 2 hours, and then filter by plate and frame pressing to obtain filter cake. Freeze the filter cake at -45℃ for 3 hours, and then freeze dry at 10Pa and -7℃ for 2 hours to obtain dried material; (3) The dried material obtained in step (2) is fed into an extraction vessel for supercritical CO2 extraction. The process conditions are: extraction pressure 12 MPa, extraction temperature 45℃, and CO2 flow rate 15 m³ / h. 3 / h, extraction time 2h; primary separation pressure 8MPa, separation temperature 50℃; secondary separation pressure 6MPa, separation temperature 50℃; to obtain Alpinia oxyphylla extract.

[0044] Example 2 This embodiment provides a method for extracting an extract that enhances intelligence, the method comprising the following steps: (1) Take 1 kg of cleaned fresh Alpinia oxyphylla fruit, crush it and add 1 L of acetate-sodium acetate buffer solution (pH 5.0), homogenize for 2 min, add 5 g of cellulase, 5 g of pectinase and 10 g of activated carbon, stir and react for 3 h at 35 °C to obtain the initial extract.

[0045] (2) Add 20g of diatomaceous earth to the initial extract obtained in step (1), stir for 2 hours, and then filter by plate and frame pressing to obtain filter cake. Freeze the filter cake at -40℃ for 3 hours, and then freeze-dry it at 10Pa and 5℃ for 3 hours to obtain dried material; (3) The dried material obtained in step (2) is put into the extraction vessel for supercritical CO2 extraction. The process conditions are: extraction pressure 10 MPa, extraction temperature 40℃, CO2 flow rate 10 m³ / h, extraction time 3 h; first separation pressure is 6 MPa, separation temperature is 45℃; second separation pressure is 5 MPa, separation temperature is 45℃; and Alpinia oxyphylla extract is obtained.

[0046] Example 3 This embodiment provides a method for extracting an extract that enhances intelligence, the method comprising the following steps: (1) Take 1 kg of cleaned fresh Alpinia oxyphylla fruit, crush it and add 1 L of citric acid-sodium hydroxide buffer solution (pH 6.0), homogenize for 2 min, add 10 g of β-glucanase, 10 g of β-glucosidase and 30 g of activated carbon, stir and react for 2 h at 40 °C to obtain the initial extract.

[0047] (2) Add 50g of diatomaceous earth to the initial extract obtained in step (1), stir for 2 hours, and then filter by plate and frame pressing to obtain filter cake. Freeze the filter cake at -45℃ for 2 hours, and then freeze-dry it at 15Pa and 0℃ for 3 hours to obtain dried material; (3) The dried material obtained in step (2) is fed into an extraction vessel for supercritical CO2 extraction. The process conditions are: extraction pressure 15 MPa, extraction temperature 50 °C, and CO2 flow rate 20 m³ / h. 3 / h, extraction time 2h; primary separation pressure 10MPa, separation temperature 55℃; secondary separation pressure 7MPa, separation temperature 55℃; to obtain Alpinia oxyphylla extract.

[0048] Example 4 Compared with Example 1, the difference is that "papain 5g, cellulase 10g" in step (1) is replaced with "papain 10g, cellulase 10g", while the other step parameters are the same as in Example 1.

[0049] Comparative Example 1 Compared with Example 1, the difference is that activated carbon is not added, while all other step parameters are the same as in Example 1.

[0050] Comparative Example 2 Compared with Example 1, the difference is that diatomaceous earth is not added in step (2), and the freeze-drying time at 10 Pa and -7 °C is changed from 2 h to 10 h. All other step parameters are the same as those in Example 1.

[0051] Comparative Example 3 Compared with Example 1, the difference is that the drying method "freeze drying" in step (2) is replaced with hot air drying at 50°C for 48 hours, while the other step parameters are the same as in Example 1.

[0052] Comparative Example 4 Compared with Example 1, the difference is that "papain 5g and cellulase 10g" in step (1) are replaced with "papain 15g", while the other step parameters are the same as in Example 1.

[0053] Comparative Example 5 Compared with Example 1, the difference is that "papain 5g and cellulase 10g" in step (1) are replaced with "cellulase 15g", while the other step parameters are the same as in Example 1.

[0054] Comparative Example 6 Compared with Example 1, the difference is that no enzyme is added in step (1), while the parameters of other steps are the same as those in Example 1.

[0055] Comparative Example 7 Compared with Example 1, the difference is that "papain 5g, cellulase 10g" in step (1) is replaced with "papain 5g, bromelain 10g", while the other step parameters are the same as in Example 1.

[0056] Comparative Example 8 Compared with Example 4, the difference is that "papain 10g, cellulase 10g" in step (1) is replaced with "papain 10g, bromelain 10g", while the other step parameters are the same as in Example 4.

[0057] Comparative Example 9 Comparative Example 9 describes the preparation of Alpinia oxyphylla extract using a traditional steam distillation method, which includes the following steps: Take 1 kg of cleaned fresh Alpinia oxyphylla fruit, crush it, add 1 L of citrate-disodium hydrogen phosphate buffer solution (pH 5.5), homogenize for 2 min, add 5 g of papain and 10 g of cellulase, stir and react at 40℃ for 2 h to obtain the initial extract; place the initial extract into a volatile oil extractor, steam distill for 8 h, collect the crude extract obtained by distillation, remove water with anhydrous sodium sulfate, and take out the oil layer to obtain Alpinia oxyphylla extract.

[0058] Comparative Example 10 The main difference compared to Example 1 is that activated carbon and diatomaceous earth are added after freeze-drying and supercritical CO2 extraction.

[0059] Specifically, the steps include the following: (1) Take 1 kg of cleaned fresh Alpinia oxyphylla fruit, crush it and add 1 L of citrate-disodium hydrogen phosphate buffer solution (pH 5.5), homogenize for 2 min, add 5 g of papain and 10 g of cellulase, stir and react for 2 h at 40 °C to obtain the initial extract; freeze the initial extract at -45 °C for 3 h, and then freeze dry at 10 Pa and -7 °C for 24 h (no plate and frame filtration was performed, so the water content was high and the freeze drying time was appropriately extended) to obtain the dried material; (2) The dried material obtained in step (1) is fed into an extraction vessel for supercritical CO2 extraction. The process conditions are: extraction pressure 12 MPa, extraction temperature 45℃, and CO2 flow rate 15 m³ / h. 3 / h, extraction time 2h; primary separation pressure 8MPa, separation temperature 50℃; secondary separation pressure 6MPa, separation temperature 50℃; obtained unbleached Alpinia oxyphylla extract.

[0060] (3) Add 20g of activated carbon and 30g of diatomaceous earth to the unbleached Alpinia extract, stir at room temperature for 2 hours, filter to obtain the filtrate, and obtain Alpinia extract.

[0061] Comparative Example 11 The main difference compared to Example 1 is that diatomaceous earth is added after freeze-drying and supercritical CO2 extraction.

[0062] Specifically, the steps include the following: (1) Take 1 kg of cleaned fresh Alpinia oxyphylla fruit, crush it and add 1 L of citrate-disodium hydrogen phosphate buffer solution (pH 5.5), homogenize for 2 min, add 5 g of papain, 10 g of cellulase and 20 g of activated carbon, stir and react for 2 h at 40 °C to obtain the initial extract; freeze the initial extract at -45 °C for 3 h, and then freeze dry at 10 Pa and -7 °C for 24 h to obtain the dried material; (2) The dried material obtained in step (1) is fed into an extraction vessel for supercritical CO2 extraction. The process conditions are: extraction pressure 12 MPa, extraction temperature 45℃, and CO2 flow rate 15 m³ / h. 3 / h, extraction time 2h; primary separation pressure 8MPa, separation temperature 50℃; secondary separation pressure 6MPa, separation temperature 50℃; obtained decolorized Alpinia oxyphylla extract.

[0063] (3) Add 30g of diatomaceous earth to the decolorized Alpinia extract obtained in step (2), stir at room temperature for 2 hours, filter to obtain the filtrate, and obtain Alpinia extract.

[0064] Comparative Example 12 Compared with Example 1, the main difference is that the diatomaceous earth in step (2) is replaced with quartz sand, while the parameters of other steps are the same as those in Example 1.

[0065] Characterization of the Alpinia extract from the experimental example I. Experimental Methods (1) Determination of yield Collect and weigh the content of Alpinia oxyphylla extracts prepared in each example and comparative example, and calculate the yield of Alpinia oxyphylla extract according to the following formula: Yield (g / kg) = mass of Alpinia oxyphylla extract (g) / mass of Alpinia oxyphylla used for extraction (kg).

[0066] (2) Judgment of smell and color Color: Inject the prepared Alpinia oxyphylla extract sample into a colorimetric tube, let it stand until there are no bubbles, and observe the color of the Alpinia oxyphylla extract under a white background plate.

[0067] Odor: Dip the scent identification paper into the sample (aleurone extract) for about 1 cm to collect the sample. After scraping off as much excess liquid as possible at the mouth of the container, remove the scent identification paper and then keep the scent identification paper 2 cm away from your nose to evaluate its aroma.

[0068] (3) Determination of repair efficacy The repair effects of the Alpinia oxyphylla extracts obtained in the examples and comparative examples were evaluated using the following methods: 1. Sample Preparation: The Alpinia oxyphylla extracts prepared in Examples 1-4 and Comparative Examples 1-12 were diluted to 0.05 wt% with standard dilution water and used as samples for testing. The preparation of standard dilution water was based on group standard T / ZHCA016—2022.

[0069] 2. Zebrafish Preparation: Wild zebrafish that were 3 days post-fertilization and in good condition were randomly selected. Fifteen zebrafish were left untreated, while the remaining zebrafish underwent surgically to quantitatively sever their caudal fins for modeling. The treated and untreated zebrafish were randomly assigned to 6-well plates, with 15 zebrafish per well. One well containing untreated zebrafish served as the normal control group; 16 wells containing treated zebrafish served as the sample group; and one well containing treated zebrafish served as the model group.

[0070] 3. Sample preparation: Add 3 mL of each sample to one of the 16 sample groups; add 3 mL of standard dilution water to the normal control group and the model group. Then incubate the six-well plate at 28°C in the dark for 48 h.

[0071] 4. Results Analysis: Ten zebrafish were randomly selected from each experimental group and photographed under a dissecting microscope. Advanced image processing software was used to analyze and collect data. The area (A) of the zebrafish's tail fin was analyzed, and the average area was taken. The following formula was used to calculate and determine whether the sample had a repairing effect: .

[0072] (4) Determination of soothing effect The soothing effects of the Alpinia extracts obtained in the examples and comparative examples were evaluated using the following methods: 1. Zebrafish preparation: Transgenic neutrophil green fluorescent zebrafish, two days after fertilization, were randomly selected and placed in a 6-well plate, with 15 zebrafish in each well.

[0073] 2. Zebrafish were divided into three groups: a normal control group (1 well), a model group (1 well), and a sample group (16 wells). The normal control group received only 3 mL of standard dilution water; the model group received 3 mL of modeling agent (60 μg / mL sodium dodecyl sulfonate); and the sample group received 3 mL of a mixed solution of sodium dodecyl sulfonate and the sample, with the sodium dodecyl sulfonate concentration at 60 μg / mL and the sample concentration at 0.05 wt%, using standard dilution water as the solvent. All zebrafish were incubated at 28°C in the dark for 18 hours. The preparation of the modeling agent and standard dilution water followed the group standard T / ZHCA 016—2022.

[0074] 3. Ten zebrafish were randomly selected from each experimental group and photographed under a fluorescence microscope. Advanced image processing software was used to analyze and collect data. The number of neutrophils (N) in the zebrafish skin was analyzed, and the average value was taken. The following formula was used to calculate and determine whether the sample had a soothing effect.

[0075]

[0076] II. Test Results Table 1. Determination results of the Alpinia oxyphylla extract obtained from different examples and comparative examples.

[0077] The effect of the Alpinia oxyphylla extract obtained in Example 1 on the regeneration ability of zebrafish tail fins is as follows: Figure 1 As shown, compared to the model group, it can significantly increase the area of ​​the zebrafish's tail fin and has a better repair effect.

[0078] As shown in Table 1, Examples 1-4 utilize specific types of enzymes for enzymatic hydrolysis, combined with activated carbon and diatomaceous earth for impurity removal, freeze drying, and supercritical extraction processes. The extraction temperature does not exceed 60°C throughout the process, which can reduce essential oil oxidation, increase the yield of the Alpinia extract, and obtain essential oil products with a fresh and rich aroma and good soothing and repairing effects.

[0079] As shown in Example 1 and Comparative Example 1, the addition of activated carbon can adsorb impurities and pigments, thereby improving the yield and purity of the Alpinia oxyphylla extract, and thus achieving better soothing and repairing effects. As shown in Example 1 and Comparative Example 2, the addition of diatomaceous earth not only aids filtration and reduces the water content in the extract, but also acts as a sample carrier to disperse the Alpinia oxyphylla raw materials, thereby shortening the freeze-drying time and further improving the yield of the Alpinia oxyphylla extract, achieving better soothing and repairing effects. As shown in Examples 1, 4, and Comparative Examples 4-8, the specific enzyme combination selected in this invention can improve the yield of the Alpinia oxyphylla extract as well as its soothing and repairing effects. As shown in Examples 1, 3, and 9, high-temperature extraction easily causes oxidation of the essential oils in the extract, reducing the yield of the Alpinia oxyphylla extract, and the resulting Alpinia oxyphylla extract also has poorer soothing and repairing effects. Based on Examples 1, 10, and 11, it can be seen that adding activated charcoal and diatomaceous earth before freeze-drying can further improve the yield of Alpinia oxyphylla extract. In addition, adding activated charcoal and diatomaceous earth before freeze-drying also results in essential oils with better repairing and soothing effects. Based on Examples 1 and 12, it can be seen that the combination of activated charcoal and diatomaceous earth is more effective than that of activated charcoal and quartz sand. This is mainly because the initial extract loses some fruit particles after being treated with quartz sand, reducing the yield of essential oils. Furthermore, quartz sand and diatomaceous earth differ in their adsorption of aroma components.

[0080] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any changes, modifications, substitutions, combinations, or simplifications made without departing from the spirit and principle of the present invention shall be considered equivalent substitutions and shall be included within the protection scope of the present invention.

Claims

1. A method for extracting an intelligence-boosting extract, characterized in that, Includes the following steps: S1. Mix Alpinia oxyphylla fruit or kernel with a buffer solution with pH 5-6, homogenize and crush, then add compound enzyme and activated carbon, and fully enzymatically hydrolyze and decolorize to obtain the initial extract. S2. Add diatomaceous earth to the crude extract obtained in step S1, stir thoroughly, filter under pressure, take the filter cake and freeze dry to obtain dried material; S3. The dried material obtained in step S2 is subjected to supercritical CO2 extraction to obtain Alpinia extract; The complex enzyme is selected from two or more of papain, cellulase, pectinase, hemicellulase, glucanase, and β-glucosidase.

2. The extraction method according to claim 1, characterized in that, The amount of the compound enzyme added is 1.0wt% to 2.0wt% of Alpinia oxyphylla fruit or Alpinia oxyphylla kernel.

3. The extraction method according to claim 1, characterized in that, The complex enzyme is a combination of papain and cellulase, with a mass ratio of papain to cellulase of 1:(1~3).

4. The extraction method according to claim 1, characterized in that, The extraction method includes one or both of the following (1) to (2): (1) The amount of activated carbon added is 1wt% to 3wt% of Alpinia oxyphylla fruit or Alpinia oxyphylla kernel; (2) The amount of diatomaceous earth added is 2.0wt% to 5.0wt% of Alpinia oxyphylla fruit or Alpinia oxyphylla kernel.

5. The extraction method according to claim 1, characterized in that, The freeze-drying step involves first freezing at -45~-40℃ for 2~3 hours, and then drying at -7~5℃ and 10~15Pa for 2~3 hours.

6. The extraction method according to claim 1, characterized in that, The parameters of the supercritical CO2 extraction include one or more of the following (1) to (4): (1) The extraction pressure is 10~15MPa, and the extraction temperature is 40~50℃; (2) The CO2 flow rate is 10~20m³ / h. 3 / h, extraction time is 2~3h; (3) primary separation pressure is 6~10MPa, separation temperature is 45~55℃; (4) secondary separation pressure is 5~7MPa, separation temperature is 45~55℃.

7. The extraction method according to claim 1, characterized in that, The temperature for complete enzymatic hydrolysis and decolorization is 35~40℃.

8. The extraction method according to claim 1, characterized in that, The buffer solution includes at least one of citrate-disodium hydrogen phosphate, acetic acid-sodium acetate, and citrate-sodium hydroxide.

9. The Alpinia extract prepared by the extraction method according to any one of claims 1 to 8.

10. The use of the Alpinia oxyphylla extract prepared by the extraction method according to claim 9 in the preparation of one or more cosmetics, food, or health products.

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