A composition with antioxidant function and exosome structure protection and a preparation method and application thereof

Abstract

The application discloses a composition with antioxidant function and exosome structure protection effect and a preparation method and application thereof. The composition with antioxidant function and exosome structure protection effect is prepared by compounding gynostemma pentaphyllum exosomes with an exosome protective agent, a first preservative and other components to form a stable composite system, which not only effectively protects the gynostemma pentaphyllum exosome structure, but also obviously improves the antioxidant capacity of the gynostemma pentaphyllum exosomes. The composition still has a regular shape, a clear boundary and a tea tray-shaped vesicle structure under long-time placement at 45 DEG C, and a cosmetic prepared by using the composition has an obvious effect of improving cell viability, which indicates that the composition has excellent structure stability and antioxidant function, and provides important data support for developing a cosmetic with more excellent antioxidant efficacy.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and in particular to a composition having antioxidant function and exosome structure protection function, its preparation method and application. Background Technology

[0002] As the largest organ in the human body, the skin is constantly exposed to a complex external environment, facing attacks from various oxidative stressors such as ultraviolet radiation and air pollution. These oxidative stressors cause skin cells to produce a large number of reactive oxygen species (ROS). When the production of ROS exceeds the skin's own antioxidant system's ability to scavenge them, lipid peroxidation occurs, damaging the integrity of skin cell membranes and leading to the degradation of collagen and elastin fibers. This results in signs of aging such as dryness, roughness, increased wrinkles, pigmentation, and sagging skin. Therefore, it is urgent to develop effective antioxidant strategies to delay skin aging.

[0003] Exosomes are lipid bimembrane vesicles secreted by cells, approximately 30-150 nm in diameter, containing various bioactive components such as proteins, lipids, nucleic acids, and small molecule metabolites. They can deliver these bioactive components to target cells through membrane fusion or endocytosis, participating in physiological processes such as intercellular communication, regulating cell proliferation and differentiation, and promoting tissue repair, demonstrating significant application potential in the fields of antioxidation and repair. Gynostemma pentaphyllum, a plant used both as food and medicine, has exosomes rich in flavonoids, triterpenoid saponins, polysaccharides, and other bioactive components, possessing biological functions such as scavenging free radicals and inhibiting lipid peroxidation, exhibiting unique advantages in antioxidation and repairing damaged skin barriers. However, the application of Gynostemma pentaphyllum exosomes in the cosmetic industry still faces serious challenges: firstly, during cosmetic production, processes such as emulsification, homogenization, and stirring may damage the vesicle structure of Gynostemma pentaphyllum exosomes, leading to leakage of their contained bioactive components and loss of their biological functions; secondly, factors such as enzymes and pH levels on the skin surface may accelerate the degradation of Gynostemma pentaphyllum exosomes, resulting in a decline in their antioxidant function.

[0004] Therefore, improving the structural stability and antioxidant function of Gynostemma pentaphyllum exosomes is of great significance for promoting their application in the cosmetics field. Summary of the Invention

[0005] This invention provides a composition with antioxidant function and exosome structure protection function. The composition has excellent structural stability and antioxidant function. Cosmetics prepared using this composition have a significant effect on improving cell vitality.

[0006] The present invention also provides a cosmetic comprising the above-mentioned composition having antioxidant function and exosome structure protection function, thus the cosmetic having the effect of improving cell vitality.

[0007] The present invention also provides a method for preparing a cosmetic product, which can be used to prepare the above-mentioned cosmetic product with the function of improving cell vitality.

[0008] The first aspect of the present invention provides a composition having antioxidant function and exosome structure protection function, the composition comprising the following components by weight: 65-75 parts of Gynostemma pentaphyllum exosomes, 0.2-1.5 parts of exosome protectant, 0.8-3.2 parts of first preservative, and the balance being water, wherein the sum of the weight parts of the above components is 100 parts.

[0009] The composition described above, which has antioxidant function and exosome structure protection, wherein the exosome protectant is at least one of carbohydrate isomers, cellulose gum, xanthan gum, and pectin.

[0010] In the composition described above that has antioxidant function and exosome structure protection, the first preservative is at least one of polyols, p-hydroxyacetophenones, and parabens.

[0011] The composition described above, which has antioxidant function and exosome structure protection, wherein the polyol is at least one of 1,2-pentanediol and 1,2-hexanediol.

[0012] The composition described above, which possesses antioxidant and exosome structure protection functions, is obtained by a preparation method comprising the following steps: An exosome protectant solution was prepared by dissolving the exosome protectant in water. While stirring, the exosome preservative solution is added to the Gynostemma pentaphyllum exosomes. After stirring evenly, the first preservative is added, and stirring is continued until all components are mixed evenly and the mixture becomes clear, thus obtaining the clear and homogeneous composition.

[0013] A second aspect of the present invention provides a cosmetic product comprising, by weight, the following components: 1.5-8 parts of a composition having antioxidant function and exosome structure protection function, 4-6 parts of an emulsifier, 8-12 parts of a moisturizer, 0.5-1 part of a thickener, 10-15 parts of a skin moisturizer, 0.1-0.3 parts of a pH adjuster, 0.8-3.2 parts of a second preservative, and the balance being water, wherein the sum of the weight parts of the above components is 100 parts; The composition having antioxidant function and exosome structure protection function is the composition having antioxidant function and exosome structure protection function described above.

[0014] In the cosmetic described above, the emulsifier is composed of a liquid crystal emulsifier and cetearyl alcohol; In the emulsifier, the weight ratio of liquid crystal emulsifier to cetearyl alcohol is (1-2):1.

[0015] In the cosmetic described above, the moisturizer is at least one of glycerin, 1,2-pentanediol, 1,2-hexanediol, 1,3-propanediol, dipropylene glycol, allantoin, betaine, and trehalose. And / or, the thickener is at least one of hydroxyethyl acrylate / sodium acryloyl dimethyl taurate copolymer, acrylate / C10-30 alkanol acrylate crosspolymer, and carbomer; And / or, the emollient is at least one of squalane, caprylic / capric triglyceride, diethylhexyl carbonate, polydimethylsiloxane, shea butter, jojoba esters, sunflower seed wax, green chamomile wax, polyglycerol-3, tocopherol, and tocopheryl acetate. And / or, the pH adjuster is any one of sodium hydroxide, triethanolamine, and arginine; And / or, the second preservative is at least one of polyols, p-hydroxyacetophenones, and parabens.

[0016] A third aspect of the present invention provides a method for preparing the cosmetic product, comprising the following steps: The emulsifier and emollient are subjected to a first stirring process to obtain the oil phase; The humectant, thickener and second preservative are premixed and dispersed to obtain a premixed phase component. The premixed phase component and water are then subjected to a second stirring treatment to obtain an aqueous phase. The oil phase and the aqueous phase are mixed and then homogenized at a first temperature to obtain a homogenized liquid at the first temperature; After cooling the homogenized liquid at the first temperature to the second temperature, a pH adjuster is added to adjust the pH to 5.6-6.2. The temperature is then further reduced to the third temperature. At the third temperature, a composition with antioxidant function and exosome structure protection function is added and subjected to a third stirring treatment to obtain the cosmetic product.

[0017] In the cosmetic preparation method described above, the temperature in the first stirring process is 80-85℃; And / or, in the second stirring treatment, the temperature is 80-85℃ and the time is 25-30 min; And / or, the first temperature is 82-85℃; in the homogenization process, the frequency is 50Hz and the time is 8-10min; And / or, the second temperature is 55-65°C; And / or, the third temperature is 40-45°C; And / or, the third stirring treatment time is 20-35 min.

[0018] The solution of the present invention has at least the following effects: The composition provided by this invention, which has antioxidant function and exosome structure protection, forms a stable composite system by combining Gynostemma pentaphyllum exosomes with key components such as exosome protectants and a first preservative. This not only effectively protects the structure of Gynostemma pentaphyllum exosomes but also significantly improves the antioxidant capacity of Gynostemma pentaphyllum exosomes. This invention specifies that the exosome protectant is at least one of saccharide isomers, cellulose gum, xanthan gum, and pectin, and that the first preservative is at least one of polyols, p-hydroxyacetophenones, and parabens. Firstly, the exosome protectant (such as saccharide isomers) can form a three-dimensional network structure, creating a physical barrier that supports and protects the Gynostemma pentaphyllum exosomes. Secondly, the exosome protectant (such as saccharide isomers) can form a protective film on the surface of the Gynostemma pentaphyllum exosomes, reducing mechanical damage to the lipid bilayer membrane caused by emulsification, homogenization, and stirring processes in cosmetic production, thus protecting the vesicle structure of the Gynostemma pentaphyllum exosomes. Thirdly, the first preservative (such as polyols) can both prevent microbial contamination of the Gynostemma pentaphyllum exosomes and avoid the damage to the structure of the exosomes caused by traditional emulsifiers (such as phenoxyethanol). Studies have shown that this composition retains its regular, well-defined, saucer-like vesicle structure even after prolonged storage at 45°C. Furthermore, cosmetics prepared using this composition significantly enhance cell viability, indicating its excellent structural stability and antioxidant function. This provides crucial data support for developing cosmetics with superior antioxidant effects. In addition, the preparation method of this composition is simple, easy to control, and suitable for large-scale production. The mixing process of the components is straightforward, requiring no special equipment and is compatible with existing cosmetic production lines, reducing production costs. Moreover, the Gynostemma pentaphyllum exosomes used are derived from natural plants, and the exosome protectant used is free of harmful chemicals, exhibiting no significant irritation to normal or sensitive skin, demonstrating high safety and broad application prospects. Attached Figure Description

[0019] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0020] Figure 1 Here is a TEM image of the composition in Example 1, wherein Figure 1 Image a is a TEM image of the initial (before acceleration) composition. Figure 1 b is a TEM image of the accelerated composition; Figure 2Here is a TEM image of the composition in Example 2, wherein Figure 2 Image a is a TEM image of the initial (before acceleration) composition. Figure 2 b is a TEM image of the accelerated composition; Figure 3 Here is a TEM image of the composition in Example 3, wherein Figure 3 Image a is a TEM image of the initial (before acceleration) composition. Figure 3 b is a TEM image of the accelerated composition; Figure 4 Here is a TEM image of the composition in Example 4, wherein Figure 4 Image a is a TEM image of the initial (before acceleration) composition. Figure 4 b is a TEM image of the accelerated composition; Figure 5 The TEM image shows the composition in Comparative Example 1, where Figure 5 Image a is a TEM image of the initial (before acceleration) composition. Figure 5 b is a TEM image of the accelerated composition; Figure 6 The TEM image shows the composition in Comparative Example 2, where Figure 6 Image a is a TEM image of the initial (before acceleration) composition. Figure 6 b is a TEM image of the accelerated composition; Figure 7 The TEM image shows the composition in Comparative Example 3, where Figure 7 Image a is a TEM image of the initial (before acceleration) composition. Figure 7 b is a TEM image of the accelerated composition; Figure 8 TEM image of the initial composition in Comparative Example 4; Figure 9 The TEM image of the cosmetic in Example 1 is shown below. Figure 9 Image 'a' is a TEM image of the cosmetics before acceleration. Figure 9 b is a TEM image of the cosmetic after acceleration; Figure 10 The images show polarized light micrographs of the emulsifier used in Example 1 (composed of Emulium Delta MB and cetearyl alcohol) and a conventional emulsifier. Figure 10 Image a is a polarized light microscope image of the emulsifier (composed of Emulium Delta MB and cetearyl alcohol) used in Application Example 1. Figure 10 b is a polarized light microscope image of a conventional emulsifier; Figure 11 These are the test results for the antioxidant function of this invention. Detailed Implementation

[0021] To make the objectives, technical solutions, and advantages of this invention clearer, the technical solutions in the embodiments of this invention will be clearly and completely described below in conjunction with the embodiments of this invention. Obviously, the described embodiments are only some embodiments of this invention, not all embodiments. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this invention. Where specific techniques or conditions are not specified in the embodiments, they shall be performed in accordance with the techniques or conditions described in the literature in this art or in accordance with the product instructions. Reagents or instruments used without specified manufacturers are all conventional products that can be obtained commercially.

[0022] In the following description, the term "and / or" is used to describe the relationship between related objects, indicating that there can be three relationships. For example, A and / or B can mean: A exists alone, B exists alone, and A and B exist simultaneously. A and B can be singular or plural.

[0023] In this invention, the use of terms such as "first" and "second" is for distinguishing similar objects and not for describing a specific order or sequence, and therefore should not be construed as a limitation of this invention.

[0024] Those skilled in the art should understand that, in the following description of the embodiments of the present invention, the sequence of numbers does not imply the order of execution. Some or all steps may be executed in parallel or sequentially. The execution order of each process should be determined by its function and internal logic, and should not constitute any limitation on the implementation process of the embodiments of the present invention.

[0025] Those skilled in the art will understand that the numerical ranges in the embodiments of the present invention should be understood to specifically disclose each intermediate value between the upper and lower limits of the range. Any stated value or intermediate value within a stated range, as well as each smaller range between any other stated value or intermediate value within said range, are also included in the present invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0026] Unless otherwise stated, the technical / scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains.

[0027] Gynostemma pentaphyllum exosomes are rich in flavonoids, triterpenoid saponins, polysaccharides, and other bioactive components, possessing biological functions such as scavenging free radicals and inhibiting lipid peroxidation. They exhibit unique advantages in antioxidation and repairing damaged skin barriers. However, the application of Gynostemma pentaphyllum exosomes in the cosmetic industry still faces serious challenges: First, during cosmetic production, processes such as emulsification, homogenization, and high-temperature sterilization may damage the lipid bilayer membrane structure of Gynostemma pentaphyllum exosomes, leading to leakage of their contained bioactive components and loss of their biological functions. Second, factors such as enzymes and pH levels on the skin surface may accelerate the degradation of Gynostemma pentaphyllum exosomes, resulting in a decline in their antioxidant function.

[0028] Based on this, in a first aspect, the present invention provides a composition having antioxidant function and exosome structure protection function, the composition comprising the following components by weight: 65-75 parts of Gynostemma pentaphyllum exosomes, 0.2-1.5 parts of exosome protectant, 0.8-3.2 parts of first preservative, and the balance being water, the sum of the weight parts of the above components being 100 parts.

[0029] This invention optimizes the ratio of key components such as Gynostemma pentaphyllum exosomes, exosome protectants, and a first preservative to form a stable composite system (the composition of this invention). This system not only effectively protects the structure of Gynostemma pentaphyllum exosomes but also significantly improves their antioxidant capacity. The exosome protectant forms a three-dimensional network structure, creating a physical barrier that supports and protects the exosomes. Furthermore, the exosome protectant forms a protective film on the surface of the exosomes, reducing mechanical damage to the lipid bilayer membrane caused by emulsification, homogenization, and stirring processes in cosmetic production, thus protecting the vesicle structure of the exosomes. The first preservative (such as a polyol) not only protects the exosomes from microbial contamination but also avoids the damage to the exosome structure caused by traditional preservatives (such as phenoxyethanol).

[0030] The composition provided by this invention retains a regular, clearly defined, saucer-like vesicle structure even under conditions of 45°C and long-term storage, indicating that the composition has excellent structural stability. This allows the bioactive components such as flavonoids, triterpenoid saponins, and polysaccharides contained in the Gynostemma pentaphyllum exosomes to be preserved, ensuring that they can exert their biological functions in cosmetics. Cosmetics prepared using this composition have a significant effect on improving cell vitality, indicating that the composition has excellent antioxidant function.

[0031] In one specific embodiment, the exosome protectant is at least one of carbohydrate isomers, cellulose gum, xanthan gum, and pectin, preferably a carbohydrate isomer.

[0032] When the exosome protectant is the aforementioned substance, it can form a three-dimensional network structure, creating a physical barrier that supports and protects the Gynostemma pentaphyllum exosomes. It can also form a protective film on the surface of the Gynostemma pentaphyllum exosomes, reducing mechanical damage to the lipid bilayer membrane caused by emulsification, homogenization, and stirring processes in cosmetic production. This protects the vesicle structure of the Gynostemma pentaphyllum exosomes, effectively protecting their structure and enhancing their antioxidant capacity, thus facilitating the acquisition of a composition with excellent structural stability and antioxidant function.

[0033] In one specific embodiment, the first preservative is at least one of polyols, p-hydroxyacetophenone, and parabens, preferably polyols.

[0034] When the first preservative is the aforementioned substance, it can both prevent the Gynostemma pentaphyllum exosomes from being contaminated by microorganisms and avoid the damage to the structure of Gynostemma pentaphyllum exosomes caused by traditional preservatives (such as phenoxyethanol).

[0035] Furthermore, the aforementioned polyols are at least one of 1,2-pentanediol and 1,2-hexanediol.

[0036] In one specific embodiment, the composition is obtained by a preparation method comprising the following process: An exosome protectant solution was prepared by dissolving the exosome protectant in water. While stirring, the exosome preservative solution is added to the Gynostemma pentaphyllum exosomes. After stirring evenly, the first preservative is added, and stirring is continued until all components are mixed evenly and the mixture becomes clear, thus obtaining the clear and homogeneous composition.

[0037] According to the inventors' research, the composition formed by combining Gynostemma pentaphyllum exosomes with exosome protectants and a first preservative through the above method has a regular-shaped, clearly defined, tea-cup-shaped vesicle structure. Even after being placed at 45°C for a long time, the composition still has a regular-shaped, clearly defined, tea-cup-shaped vesicle structure. Furthermore, cosmetics prepared using this composition have a significant effect on improving cell viability, indicating that the composition has excellent structural stability and antioxidant function.

[0038] In this invention, the preparation method of the composition is simple, easy to control, and can be mass-produced; the mixing process of each component is simple, requires no special equipment, is compatible with existing cosmetic production lines, and reduces production costs; at the same time, the Gynostemma pentaphyllum exosomes used are derived from natural plants, and the exosome protectants used do not contain harmful chemicals, have no obvious irritation to normal or sensitive skin, and are highly safe to use.

[0039] Secondly, the present invention provides a cosmetic product comprising, by weight, the following components: 1.5-8 parts of a composition having antioxidant function and exosome structure protection function, 4-6 parts of an emulsifier, 8-12 parts of a moisturizer, 0.5-1 part of a thickener, 10-15 parts of a skin moisturizer, 0.1-0.3 parts of a pH adjuster, 0.8-3.2 parts of a second preservative, and the balance being water, wherein the sum of the weight parts of the above components is 100 parts; wherein the composition having antioxidant function and exosome structure protection function is the aforementioned composition having antioxidant function and exosome structure protection function.

[0040] This invention optimizes the ratio of the above-mentioned composition with antioxidant function and exosome structure protection to components such as emulsifiers, moisturizers, and thickeners to obtain a cosmetic product that enhances cell vitality.

[0041] In one specific embodiment, the emulsifier is composed of a liquid crystal emulsifier and cetearyl alcohol; wherein the weight ratio of the liquid crystal emulsifier to cetearyl alcohol in the emulsifier is (1-2):1.

[0042] This invention does not specifically limit the source of the liquid crystal emulsifier and cetearyl alcohol, which can be obtained through commercial means.

[0043] In this invention, the liquid crystal emulsifier was purchased commercially and its product name is Emulium Delta MB, and its INCI name is cetyl alcohol, glyceryl stearate, PEG-75 stearate, cetyl alcohol polyether-20, stearyl alcohol polyether-20. It was purchased from Giafass.

[0044] The liquid crystal emulsifier can protect the Gynostemma pentaphyllum exosomes, while cetearyl alcohol helps in the formation of the liquid crystal structure. The fluidity of the liquid crystal structure can act as a buffer. When the Gynostemma pentaphyllum exosomes are subjected to external pressure or impact, the liquid crystal molecules in the emulsifier can disperse and absorb this energy through their own movement, thereby reducing the damage to the Gynostemma pentaphyllum exosomes and protecting the integrity and structural stability of the Gynostemma pentaphyllum exosomes to a certain extent.

[0045] For example, in the above emulsifier, the weight ratio of liquid crystal emulsifier and cetearyl alcohol can be any one of 1:1, 1.5:1, 2:1 or any combination of both.

[0046] In one specific embodiment, the humectant is at least one selected from glycerin, 1,2-pentanediol, 1,2-hexanediol, 1,3-propanediol, dipropylene glycol, allantoin, betaine, and trehalose. In one specific embodiment, the thickener is at least one of hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, acrylate / C10-30 alkanol acrylate crosspolymer, and carbomer.

[0047] In one specific embodiment, the emollient is at least one of squalane, caprylic / capric triglyceride, diethylhexyl carbonate, polydimethylsiloxane, shea butter, jojoba esters, sunflower seed wax, green chamomile wax, polyglycerol-3, tocopherol, and tocopheryl acetate.

[0048] In one specific embodiment, the pH adjuster is any one of sodium hydroxide, triethanolamine, and arginine, preferably arginine.

[0049] In one specific embodiment, the second preservative is at least one of polyols, p-hydroxyacetophenones, and parabens.

[0050] Thirdly, the present invention provides a method for preparing the cosmetic described above, comprising the following steps: The emulsifier and emollient are subjected to a first stirring process to obtain the oil phase; The humectant, thickener and second preservative are premixed and dispersed to obtain a premixed phase component. The premixed phase component and water are then subjected to a second stirring treatment to obtain an aqueous phase. The oil phase and the aqueous phase are mixed and then homogenized at a first temperature to obtain a homogenized liquid at the first temperature; After cooling the homogenized liquid at the first temperature to the second temperature, a pH adjuster is added to adjust the pH to 5.6-6.2. The temperature is then further reduced to the third temperature. At the third temperature, a composition with antioxidant function and exosome structure protection function is added and subjected to a third stirring treatment to obtain the cosmetic product.

[0051] The present invention can prepare cosmetics that improve cell vitality through the above method.

[0052] In one specific embodiment, the temperature in the first stirring process is 80-85°C.

[0053] The present invention does not impose a particular limitation on the time of the first stirring process, as long as the emulsifier and emollient are completely dissolved and mixed evenly.

[0054] In one specific embodiment, the second stirring process is carried out at a temperature of 80-85°C for 25-30 minutes.

[0055] In one specific embodiment, the first temperature is 82-85℃; in the homogenization process, the frequency is 50Hz and the time is 8-10min. Specifically, the oil phase and the aqueous phase are mixed and homogenized at a frequency of 50Hz and a temperature of 82-85℃ for 8-10min to obtain a homogenized liquid at 82-85℃.

[0056] In this invention, the purpose of homogenization is to homogenize and emulsify the mixed oil and water phases.

[0057] In one specific embodiment, the second temperature is 55-65°C.

[0058] In one specific embodiment, the third temperature is 40-45°C.

[0059] The composition, which has antioxidant function and exosome structure protection function, is an active substance. It is added at low temperature (40-45℃) to prevent its inactivation.

[0060] In one specific embodiment, the third stirring process takes 20-35 minutes.

[0061] The present invention will be further described below through specific embodiments.

[0062] In the following examples, Gynostemma pentaphyllum exosomes were purchased from MicroMed Biotechnology (Yantai) Co., Ltd.; the liquid crystal emulsifier used was Emulium Delta MB, INCI name: cetyl alcohol, glyceryl stearate, PEG-75 stearate, cetyl alcohol polyether-20, stearyl alcohol polyether-20, purchased from Gaffars; the saccharide isomers were purchased from DSM; the cellulose gum was purchased from Ashland; the xanthan gum was purchased from Azeres; the pectin was purchased from Azeres; the phenoxyethanol was purchased from Tor; the emollient used was ACTICR EMB, INCI name: jojoba esters, sunflower (HELIANTHUS ANNUUS) seed wax, green vitex (ACACIA DECURRENS) flower wax, polyglycerol-3, tocopherol (vitamin E), purchased from Gaffars; DMEM culture medium was purchased from Gibco; and avocado fruit oil was purchased from BASF.

[0063] Example 1 This embodiment provides a composition with antioxidant function and exosome structure protection function, which is composed of the following components by weight: 70 parts of Gynostemma pentaphyllum exosomes; 1 part of saccharide isomers; 3.2 parts of preservatives; 2 parts of 1,2-pentanediol and 1.2 parts of 1,2-hexanediol; the balance is water; the sum of the weight parts of the above components is 100 parts.

[0064] The preparation method of the composition with antioxidant function and exosome structure protection provided in this embodiment includes the following steps: Add one part of the sugar isomer to water and stir at 65°C for 30 minutes until the sugar isomer is completely dissolved. Cool down to below 45°C to prepare an exosome protectant solution. While stirring, add the exosome protectant solution to 70 parts of Gynostemma pentaphyllum exosomes, stir evenly, then add 2 parts of 1,2-pentanediol and 1.2 parts of 1,2-hexanediol, and continue stirring until all components are mixed evenly and the mixture is clear, thus obtaining a clear and homogeneous composition.

[0065] Example 2 The composition with antioxidant function and exosome structure protection provided in this embodiment is basically the same as that in Example 1, except that 1 part of the sugar isomer is replaced with 0.2 parts of cellulose gum.

[0066] The preparation method of the composition with antioxidant function and exosome structure protection provided in this embodiment is basically the same as that in Example 1, except that 1 part of the sugar isomer is replaced with 0.2 parts of cellulose gum.

[0067] Example 3 The composition with antioxidant function and exosome structure protection provided in this embodiment is basically the same as that in Example 1, except that 1 part of the sugar isomer is replaced with 0.2 parts of xanthan gum.

[0068] The preparation method of the composition with antioxidant function and exosome structure protection provided in this embodiment is basically the same as that in Example 1, except that 1 part of the sugar isomer is replaced with 0.2 parts of xanthan gum.

[0069] Example 4 The composition with antioxidant function and exosome structure protection provided in this embodiment is basically the same as that in Example 1, except that 1 part of the sugar isomer is replaced with 0.2 parts of pectin.

[0070] The preparation method of the composition with antioxidant function and exosome structure protection provided in this embodiment is basically the same as that in Example 1, except that 1 part of sugar isomer is replaced with 0.2 parts of pectin.

[0071] Comparative Example 1 (without exosome protectant) This comparative example provides a composition comprising, by weight, the following components: 70 parts of Gynostemma pentaphyllum exosomes; preservatives: 2 parts of 1,2-pentanediol and 1.2 parts of 1,2-hexanediol; the balance being water; the sum of the weight parts of the above components is 100 parts.

[0072] The method for preparing the composition provided in this comparative example includes the following steps: While stirring, 70 parts of Gynostemma pentaphyllum exosomes were added to water and stirred until homogeneous. Then, 2 parts of 1,2-pentanediol and 1.2 parts of 1,2-hexanediol were added, and stirring was continued until all components were mixed evenly and the mixture became clear, thus obtaining a clear and homogeneous composition.

[0073] Comparative Example 2 The composition provided in this comparative example is basically the same as that in Example 1, except that 1 part of the carbohydrate isomer is replaced with 5 parts of the carbohydrate isomer.

[0074] The preparation method of the composition provided in this comparative example is basically the same as that in Example 1, except that 1 part of the carbohydrate isomer is replaced with 5 parts of the carbohydrate isomer.

[0075] Comparative Example 3 (using conventional protective agents) The composition provided in this comparative example is basically the same as that in Example 1, except that 1 part of the sugar isomer is replaced with 1 part of trehalose.

[0076] The preparation method of the composition provided in this comparative example is basically the same as that in Example 1, except that 1 part of the sugar isomer is replaced with 1 part of trehalose.

[0077] Comparative Example 4 (using conventional preservatives) The composition provided in this comparative example is essentially the same as that in Example 1, except that the preservative consisting of 2 parts of 1,2-pentanediol and 1.2 parts of 1,2-hexanediol is replaced with 3.2 parts of phenoxyethanol.

[0078] The preparation method of the composition provided in this comparative example is basically the same as that in Example 1, except that 2 parts of 1,2-pentanediol and 1.2 parts of 1,2-hexanediol are replaced with 3.2 parts of phenoxyethanol.

[0079] Application Example 1 (using the composition of Example 1) This application example provides a cosmetic product comprising the following components by weight: The cosmetic product is composed of the following components by weight: 8 parts of the composition with antioxidant function and exosome structure protection function from Example 1; 4.7 parts of emulsifier: 2.7 parts of Emulium Delta MB, 2 parts of cetearyl alcohol; 10 parts of moisturizer: 5 parts of glycerin, 2 parts of trehalose, 3 parts of dipropylene glycol; 0.75 parts of thickener: 0.5 parts of hydroxyethyl acrylate / sodium acryloyl dimethyl taurate copolymer, 0.25 parts of acrylate / C10-30 alkanol acrylate crosspolymer; 12 parts of emollient: 2 parts of squalane, 3 parts of caprylic / capric triglyceride, 3 parts of diethylhexyl carbonate, 1 part of polydimethylsiloxane, 1 part of shea butter, and 1 part of ACTIRE MB. 1 part of tocopherol acetate; 0.2 parts of arginine; 3.2 parts of secondary preservative; 1.2 parts of 1,2-hexanediol; 2 parts of 1,2-pentanediol; the remainder is water, and the total weight of the above components is 100 parts.

[0080] This application example provides a method for preparing a cosmetic, including the following steps: (1) Add 2.7 parts of Emulium Delta MB, 2 parts of cetearyl alcohol, 2 parts of squalane, 3 parts of caprylic / capric triglyceride, 3 parts of diethylhexyl carbonate, 1 part of polydimethylsiloxane, 1 part of shea butter, 1 part of ACTIRE MB and 1 part of tocopheryl acetate to the oil phase mixing tank, start stirring, and heat the oil phase mixing tank to 85°C. Stir until all components are completely dissolved and mixed evenly to obtain the oil phase. (2) 5 parts glycerol, 2 parts trehalose, 3 parts dipropylene glycol, 0.5 parts hydroxyethyl acrylate / sodium acryloyl dimethyl taurate copolymer, 0.25 parts acrylate / C10-30 alkanol acrylate crosspolymer, 1.2 parts 1,2-hexanediol and 2 parts 1,2-pentanediol were premixed and dispersed. After being mixed evenly, the premixed phase component was obtained. Then, the premixed phase component and water were added to the aqueous phase mixing tank, the stirring was turned on, and the aqueous phase mixing tank was heated to 85°C and kept at that temperature for 30 min to obtain the aqueous phase. (3) After mixing the oil phase in step (1) and the aqueous phase in step (2), the mixture is homogenized for 10 min at a frequency of 50 Hz and a temperature of 85 ℃ to obtain a homogenized liquid. (4) After cooling the homogenized liquid in step (3) to 65°C, add 0.2 parts of arginine, adjust the pH to 6.0, continue to cool to 45°C, add 8 parts of the composition with antioxidant function and exosome structure protection function in Example 1 at 45°C, and stir for 35 minutes to obtain cosmetics.

[0081] Application Example 2 (using the composition of Comparative Example 1) The cosmetic provided in this application example is basically the same as that in application example 1, except that the 8 parts of the composition with antioxidant function and exosome structure protection function in example 1 are replaced with the 8 parts of the composition in comparative example 1.

[0082] The preparation method of the cosmetic provided in this application example is basically the same as that in application example 1, except that 8 parts of the composition with antioxidant function and exosome structure protection function in example 1 are replaced with 8 parts of the composition in comparative example 1.

[0083] Application Example 3 (using the composition of Comparative Example 2) The cosmetic provided in this application example is basically the same as that in application example 1, except that the 8 parts of the composition with antioxidant function and exosome structure protection function in example 1 are replaced with the 8 parts of the composition in comparative example 2.

[0084] The preparation method of the cosmetic provided in this application example is basically the same as that in application example 1, except that 8 parts of the composition with antioxidant function and exosome structure protection function in example 1 are replaced with 8 parts of the composition in comparative example 2.

[0085] Application Example 4 (using the composition of Comparative Example 3) The cosmetic provided in this application example is basically the same as that in application example 1, except that the 8 parts of the composition with antioxidant function and exosome structure protection function in example 1 are replaced with the 8 parts of the composition in comparative example 3.

[0086] The preparation method of the cosmetic provided in this application example is basically the same as that in application example 1, except that 8 parts of the composition with antioxidant function and exosome structure protection function in example 1 are replaced with 8 parts of the composition in comparative example 3.

[0087] Application Example 5 (using the composition of Comparative Example 4) The cosmetic provided in this application example is basically the same as that in application example 1, except that the 8 parts of the composition with antioxidant function and exosome structure protection function in example 1 are replaced with the 8 parts of the composition in comparative example 4.

[0088] The preparation method of the cosmetic provided in this application example is basically the same as that in application example 1, except that 8 parts of the composition with antioxidant function and exosome structure protection function in example 1 are replaced with 8 parts of the composition in comparative example 4.

[0089] Application Example 6 (without adding the composition) The cosmetic product provided in this application example is basically the same as that in Application Example 1, except that it does not contain 8 parts of the composition with antioxidant function and exosome structure protection function from Example 1. Specifically: Liquid crystal emulsifier 4.7 parts: Emulium Delta MB 2.7 parts, cetearyl alcohol 2 parts; humectant 10 parts: glycerin 5 parts, trehalose 2 parts, dipropylene glycol 3 parts; thickener 0.75 parts: hydroxyethyl acrylate / sodium acryloyl dimethyl taurate copolymer 0.5 parts, acrylate (ester) / C10-30 alkanol acrylate crosspolymer 0.25 parts; emollient 12 parts: squalane 2 parts, caprylic / capric triglyceride 3 parts, diethylhexyl carbonate 3 parts, polydimethylsiloxane 1 part, shea butter 1 part, ACTIRE MB 1 part, tocopheryl acetate 1 part; arginine 0.2 parts; secondary preservative 3.2 parts: 1,2-hexanediol 1.2 parts, 1,2-pentanediol 2 parts; balance is water. The total weight of the above components is 100 parts.

[0090] The cosmetic preparation method provided in this application example differs from that in application example 1 only in step (4); the rest is the same as in application example 1. (4) After cooling the homogenized liquid in step (3) to 65°C, add 0.2 parts of arginine, adjust the pH to 6.0, continue to cool to 45°C, and stir at 45°C for 35 minutes to obtain the cosmetic.

[0091] Performance testing 1. The composition with antioxidant function and exosome structure protection in Example 1 and the composition in Comparative Example 2 were subjected to osmotic pressure test. The test results are shown in Table 1.

[0092] Table 1 Osmotic pressure test results Excessive osmotic pressure can lead to the destruction of exosome vesicle structure during long-term storage. Table 1 shows that the osmotic pressure of the composition with antioxidant and exosome structure protection functions in Example 1 is 310.2 mOsm / kg, while the osmotic pressure of the composition in Comparative Example 2 is 412.5 mOsm / kg. This indicates that excessive use of saccharide isomers results in an excessively high osmotic pressure in the prepared composition system, which may lead to the destruction of the vesicle structure of Gynostemma pentaphyllum exosomes during long-term storage.

[0093] 2. The compositions with antioxidant function and exosome structure protection in Examples 1-4, the compositions in Comparative Examples 1-4, and the cosmetic in Application Example 1 were used as samples to conduct accelerated tests to evaluate the structural stability of the samples. The specific method of accelerated testing is as follows: the samples were placed in a constant temperature incubator at 45°C for 30 days.

[0094] The compositions with antioxidant and exosome structure protection functions (hereinafter referred to as "compositions") from Examples 1-4, the compositions from Comparative Examples 1-4, and the cosmetic from Application Example 1 were used as samples and accelerated tests were conducted according to the above method. Transmission electron microscopy (TEM) was performed on the samples before acceleration (initial samples) and the samples after acceleration (i.e., samples stored for 30 days). The results are as follows: Figure 1-9 As shown; Figure 1 Here is a TEM image of the composition in Example 1, wherein Figure 1 Image a is a TEM image of the initial (before acceleration) composition. Figure 1 b is a TEM image of the accelerated composition; Figure 2 Here is a TEM image of the composition in Example 2, wherein Figure 2 Image a is a TEM image of the initial (before acceleration) composition. Figure 2 b is a TEM image of the accelerated composition; Figure 3 Here is a TEM image of the composition in Example 3, wherein Figure 3 Image a is a TEM image of the initial (before acceleration) composition. Figure 3 b is a TEM image of the accelerated composition; Figure 4 Here is a TEM image of the composition in Example 4, wherein Figure 4 Image a is a TEM image of the initial (before acceleration) composition. Figure 4 b is a TEM image of the accelerated composition; Figure 5 The TEM image shows the composition in Comparative Example 1, where Figure 5 Image a is a TEM image of the initial (before acceleration) composition. Figure 5 b is a TEM image of the accelerated composition; Figure 6 The TEM image shows the composition in Comparative Example 2, where Figure 6 Image a is a TEM image of the initial (before acceleration) composition. Figure 6 b is a TEM image of the accelerated composition; Figure 7 The TEM image shows the composition in Comparative Example 3, where Figure 7 Image a is a TEM image of the initial (before acceleration) composition. Figure 7 b is a TEM image of the accelerated composition; Figure 8 TEM image of the initial composition in Comparative Example 4; Figure 9 The TEM image of the cosmetic in Example 1 is shown below. Figure 9 Image 'a' is a TEM image of the cosmetics before acceleration. Figure 9 b is a TEM image of the accelerated cosmetic.

[0095] Depend on Figure 1 As can be seen from a and b, the composition in Example 1 can be clearly observed to have regular shape and clear boundaries, forming tea tray-shaped vesicle structures before and after acceleration.

[0096] Depend on Figure 2 As can be seen from a and b, the composition in Example 2 can be clearly observed to have regular shape and clear boundaries, forming tea tray-shaped vesicle structures before and after acceleration.

[0097] Depend on Figure 3 As can be seen from a and b, the composition in Example 3 can be clearly observed to have regular shape and clear boundaries, forming tea tray-shaped vesicle structures before and after acceleration.

[0098] Depend on Figure 4 As can be seen from a and b, the composition in Example 4 can be clearly observed to have regular shape and clear boundaries, forming tea tray-shaped vesicle structures before and after acceleration.

[0099] Depend on Figure 5 As can be seen from a and b, the composition in Comparative Example 1 showed vesicle structures before acceleration, but almost no vesicle structures were observed after acceleration.

[0100] Depend on Figure 6 As shown in Table 1, the composition in Comparative Example 2 showed particle size reduction in the vesicle structure before acceleration, and the vesicle structure was significantly destroyed after acceleration, with almost no vesicle structure observed. This indicates that when the osmotic pressure is too high, the Gynostemma pentaphyllum exosomes are prone to deformation (the vesicle structure shows particle size reduction). Under conditions of excessive osmotic pressure and long-term storage at 45°C, the Gynostemma pentaphyllum exosomes are easily destroyed.

[0101] Depend on Figure 7 As can be seen from a and b, the composition in Comparative Example 3 showed vesicle structures before acceleration, while the vesicle structures were slightly damaged after acceleration.

[0102] Depend on Figure 8 It can be seen that the composition in Comparative Example 4 had slight damage to its vesicle structure at the beginning, and no accelerated test was performed.

[0103] Depend on Figure 9 As can be seen from a and b, the cosmetics in Example 1 can be clearly observed to have regular-shaped, clearly defined, tea-cup-shaped vesicle structures before and after acceleration.

[0104] The above results indicate that the composition provided in the embodiments of the present invention and the cosmetics made using the composition can be stored at 45°C for a long time and have excellent structural stability.

[0105] 3. The emulsifier corresponding to Example 1 (composed of Emulium Delta MB and cetearyl alcohol) and the conventional emulsifier (product name: Tego CARE PBS 6 MB, INCI name: polyglycerol-6 stearate and polyglycerol-6 behenate, purchased from Evonik) were tested by polarizing light microscopy respectively; Figure 10 The images show polarized light micrographs of the emulsifier used in Example 1 (composed of Emulium DeltaMB and cetearyl alcohol) and a conventional emulsifier. Figure 10 Image a is a polarized light microscope image of the emulsifier (composed of Emulium Delta MB and cetearyl alcohol) used in Application Example 1. Figure 10 b is a polarized light microscope image of a conventional emulsifier.

[0106] Depend on Figure 10 As can be seen from ab, the emulsifier in Application Example 1 of the present invention has a liquid crystal structure exhibiting a Maltese cross under a polarizing microscope, while conventional emulsifiers do not show a liquid crystal structure under a polarizing microscope.

[0107] 4. Antioxidant Function Test The cosmetics used in Examples 1-6 were used as samples for antioxidant function testing, and the specific methods are as follows: Human skin fibroblasts (HFB cells, purchased from Guangdong Boxi Biotechnology Co., Ltd.) were taken from the liquid nitrogen tank, thawed, and seeded into culture flasks. They were cultured in an incubator at 37°C and 5% CO2. When the cell confluence reached 75%, they were digested and passaged with trypsin (purchased from Gibco). When passaged to the 4th generation (P4), P4 generation cells were obtained. P4 generation cells were seeded into 96-well plates of each group (blank control group, model group, and sample group) at a seeding density of 8000 cells / well and incubated in an incubator at 37°C and 5% CO2. Experimental groups: blank control group, model group, and sample group. (1) Blank control group: 200 μL DMEM culture medium; (2) Model group: 200 μL DMEM medium; (3) Sample group: 200 μL of DMEM culture medium containing 1% of the sample by mass; After incubation at 37℃ and 5% CO2 for 24 hours, except for the blank control group, each well of the other groups was added with 10 μL of DMEM medium containing 400 μmol (final concentration) hydrogen peroxide. After incubation at 37℃ and 5% CO2 for another 24 hours, the supernatant was discarded, and 100 μL of 10% CCK-8 detection solution was added. The cells were incubated at 37℃ in the dark for 2 hours. After the dark incubation, 90 μL was aspirated as the detection solution, and the OD value of the detection solution was read at 450 nm. Cell viability was calculated according to the formula. Cell viability value of sample group = OD value of sample group / OD value of blank control group × 100%; Cell viability value of the model group = OD value of the model group / OD value of the blank control group × 100%; The cosmetics used in Examples 1-6 were used as samples to test their antioxidant function according to the above method. The results are as follows: Figure 11 As shown, the results are summarized in Table 2.

[0108] Table 2 Cell viability values Depend on Figure 11 As shown in Table 2, the antioxidant function of the cosmetics is related to the integrity of the vesicle structure of Gynostemma pentaphyllum exosomes. Compared with the model group, the cosmetic in Application Example 1 significantly improved cell viability; the cosmetics in Application Examples 2, 3, and 4 also improved cell viability, but significantly less than the cosmetic in Application Example 1; while the cell viability values ​​of the cosmetics in Application Examples 5 and 6 were lower than those of the model group. These results indicate that the cosmetics prepared using the compositions provided in the embodiments of the present invention significantly improve cell viability, and the compositions possess excellent antioxidant function.

[0109] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A composition having antioxidant function and exosome structure protection function, characterized in that, The composition comprises the following components by weight: 65-75 parts of Gynostemma pentaphyllum exosomes, 0.2-1.5 parts of exosome protectant, 0.8-3.2 parts of first preservative, and the balance being water, with the sum of the weight of the above components being 100 parts.

2. The composition with antioxidant function and exosome structure protection function according to claim 1, characterized in that, The exosome protectant is at least one of the following: saccharide isomers, cellulose gum, xanthan gum, and pectin.

3. The composition with antioxidant function and exosome structure protection function according to claim 1, characterized in that, The first preservative is at least one of polyols, p-hydroxyacetophenones, and parabens.

4. The composition with antioxidant function and exosome structure protection function according to claim 3, characterized in that, The polyol is at least one of 1,2-pentanediol and 1,2-hexanediol.

5. The composition with antioxidant function and exosome structure protection function according to any one of claims 1-4, characterized in that, The composition is obtained by a preparation method comprising the following process: An exosome protectant solution was prepared by dissolving the exosome protectant in water. While stirring, the exosome preservative solution is added to the Gynostemma pentaphyllum exosomes. After stirring evenly, the first preservative is added, and stirring is continued until all components are mixed evenly and the mixture becomes clear, thus obtaining the clear and homogeneous composition.

6. A cosmetic product, characterized in that, The cosmetic comprises the following components by weight: 1.5-8 parts of a composition with antioxidant function and exosome structure protection function, 4-6 parts of emulsifier, 8-12 parts of moisturizer, 0.5-1 part of thickener, 10-15 parts of emollient, 0.1-0.3 parts of pH adjuster, 0.8-3.2 parts of secondary preservative, and the balance being water, wherein the sum of the weight parts of the above components is 100 parts; The composition having antioxidant function and exosome structure protection function is the composition having antioxidant function and exosome structure protection function as described in any one of claims 1-5.

7. The cosmetic product according to claim 6, characterized in that, The emulsifier is composed of a liquid crystal emulsifier and cetearyl alcohol; In the emulsifier, the weight ratio of liquid crystal emulsifier to cetearyl alcohol is (1-2):

1.

8. The cosmetic product according to claim 6, characterized in that, The moisturizer is at least one of glycerin, 1,2-pentanediol, 1,2-hexanediol, 1,3-propanediol, dipropylene glycol, allantoin, betaine, and trehalose. And / or, the thickener is at least one of hydroxyethyl acrylate / sodium acryloyl dimethyl taurate copolymer, acrylate / C10-30 alkanol acrylate crosspolymer, and carbomer; And / or, the emollient is at least one of squalane, caprylic / capric triglyceride, diethylhexyl carbonate, polydimethylsiloxane, shea butter, jojoba esters, sunflower seed wax, green chamomile wax, polyglycerol-3, tocopherol, and tocopheryl acetate. And / or, the pH adjuster is any one of sodium hydroxide, triethanolamine, and arginine; And / or, the second preservative is at least one of polyols, p-hydroxyacetophenones, and parabens.

9. A method for preparing a cosmetic according to any one of claims 6-8, characterized in that, Includes the following steps: The emulsifier and emollient are subjected to a first stirring process to obtain the oil phase; The humectant, thickener and second preservative are premixed and dispersed to obtain a premixed phase component. The premixed phase component and water are then subjected to a second stirring treatment to obtain an aqueous phase. The oil phase and the aqueous phase are mixed and then homogenized at a first temperature to obtain a homogenized liquid at the first temperature; After cooling the homogenized liquid at the first temperature to the second temperature, a pH adjuster is added to adjust the pH to 5.6-6.

2. The temperature is then further reduced to the third temperature. At the third temperature, a composition with antioxidant function and exosome structure protection function is added and subjected to a third stirring treatment to obtain the cosmetic product.

10. The method for preparing the cosmetic according to claim 9, characterized in that, In the first stirring process, the temperature is 80-85℃; And / or, in the second stirring treatment, the temperature is 80-85℃ and the time is 25-30 min; And / or, the first temperature is 82-85℃; in the homogenization process, the frequency is 50Hz and the time is 8-10min; And / or, the second temperature is 55-65°C; And / or, the third temperature is 40-45°C; And / or, the third stirring treatment time is 20-35 min.

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