A multi-stage nano-reactor array-based nanochannel and a preparation method thereof
By preparing sulfide-bridged periodic mesoporous organosilicon PMO and loading gold nanoparticles (AuNPs), a nanoreactor Au-PMO was constructed. This solved the problem of the single nature of nanochannel materials, realized the functionalization and stability of nanochannels, and provided a new material for catalysis and sensing.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- GUANGHUA CHUANGXIN INTELLIGENT TECHNOLOGY (HANGZHOU) CO LTD
- Filing Date
- 2026-03-05
- Publication Date
- 2026-06-09
Smart Images
Figure CN122164494A_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of nano-ion channel technology, specifically, it relates to a multi-level nanoreactor array-based nanochannel and its preparation method. Background Technology
[0002] Inspired by the selective transport of specific molecules and ions through ion channels in natural biomembrane systems, researchers have developed biomimetic nanochannels with stable mechanical properties and tunable chemical properties. These channels, due to their excellent ion transport characteristics, are widely used in various fields such as ion sieving, sensing, energy conversion, ultrafiltration, and desalination. Especially in sensing applications, based on the nanoconfining effect, highly sensitive analytical detection can be achieved by real-time monitoring of changes in ion transport signals.
[0003] However, current nanochannel materials are relatively limited, and preparation techniques are restricted. Achieving the integration and functionalization of nanochannel materials and their properties, and constructing stable nanochannels for molecular recognition and detection, remains a challenge. Faced with these bottlenecks, there is an urgent need to develop methods and technologies for preparing nanoparticle channel materials with novel structures and properties for sensing. Summary of the Invention
[0004] To address the aforementioned problems, the present invention aims to provide a method for preparing nanochannels based on a multi-level nanoreactor array. This invention first prepares a thioether-bridged periodic mesoporous organosilicon (PMO), then loads gold nanoparticles (AuNPs) onto the PMO using an in-situ reduction method to construct an Au-PMO nanoreactor. Subsequently, using a multi-element assembly strategy, an Au-PMO layer is constructed on the surface of porous anodic alumina (AAO) via spin coating to obtain a nanochannel film. The preparation method is simple, the obtained multi-level nanoreactor array-based nanochannels have uniformly distributed gold nanoparticles, and the film exhibits peroxidase-like activity, making it suitable for catalysis and sensing in nanochannel devices.
[0005] The objective of this invention is achieved through the following technical solutions.
[0006] This invention provides a method for preparing nanochannels based on a multi-level nanoreactor array, the specific steps of which are as follows: (1) First, thioether-bridged periodic mesoporous organosilicon PMO nanoparticles with a shell-core structure were prepared by a one-step hydrothermal synthesis method. (2) The prepared PMO particles were dispersed in water, and a certain concentration of HAuCl4 solution was added. Then, the pH of the solution was adjusted to carry out an in-situ reduction experiment. The resulting mixed solution was centrifuged and washed to obtain a mesoporous organosilicon Au-PMO nanoreactor loaded with gold nanoparticles; (3) Disperse the mesoporous organosilicon Au-PMO nanoreactor loaded with gold nanoparticles in an organic solution containing a binder to obtain a uniform dispersion. (4) Spin-coating the dispersion onto a porous anodic aluminum oxide (AAO) membrane and then vacuum drying to obtain a composite nanochannel film.
[0007] In this invention, the specific preparation steps of the shell-core structured sulfide-bridged periodic mesoporous organosilicon PMO nanoparticles in step (1) are as follows: ①Preparation of template agent solution: Add 0.1-0.2g of quaternary ammonium salt cationic surfactant cetyltrimethylammonium bromide (CTAB) to an ethanol aqueous solution containing ammonia (0.8-1.2mL ammonia, 25-35mL ethanol, 70-80mL deionized water), and stir at 30℃-40℃. ② Mix 0.05-0.20 mL of bis[3-(triethoxysilyl)propyl]tetrasulfide (TESPTS) and 0.1-0.4 mL of tetraethyl orthosilicate (TEOS), and add the mixture to the solution obtained in step ① while stirring. Continue stirring at 30℃-40℃ for 20-30 h. ③ Centrifuge and wash the mixture obtained in step ② to obtain nanospheres; ④ Disperse the nanospheres obtained in step ③ in 20-40 mL of deionized water, and obtain shell-core structured nanoparticles by hydrothermal reaction, wherein the hydrothermal reaction temperature is 150℃-180℃ and the hydrothermal reaction time is 10-16 h; ⑤ By repeatedly washing the nanoparticles in a hydrochloric acid-ethanol solution at a temperature of 60℃-90℃ to remove the template agent, the shell-core structured thioether-bridged periodic mesoporous organosilicon PMO nanoparticles were finally obtained. The hydrochloric acid-ethanol solution was prepared by mixing concentrated hydrochloric acid and ethanol in a volume ratio of 1:450-1:550.
[0008] In this invention, in step (2), PMO particles are dispersed in water to obtain a PMO dispersion with a concentration of 1-5 mg / mL, the concentration of HAuCl4 solution is 2.4 mmol / L-4.8 mmol / L, and the volume ratio of HAuCl4 solution to PMO dispersion is 3:5-1:1.
[0009] In this invention, in step (2), during the in-situ reduction experiment, the pH range of the solution is adjusted to 7-10 using 0.8-1.2 mol / L NaOH solution, and the mixture is continuously stirred at room temperature for 12-36 h; the size of the gold nanoparticles is between 4-20 nm; the sample obtained by centrifugation is washed with deionized water. During the in-situ reduction experiment, no external reagents are added. The mesoporous structure of PMO can prevent the nanoparticles from agglomerating, and the thioether groups can anchor the gold precursor and oxidize it into sulfur oxides.
[0010] In this invention, in step (3), the Au-PMO nanoreactor is dispersed into an organic solution containing a binder under ultrasonic treatment; the ultrasonic treatment time is 1-3 hours, and the ultrasonic treatment time has a certain influence on the dispersion degree of the dispersion and the smoothness of the film.
[0011] In this invention, in step (3), the organic solution containing the binder is an acetone solution of polyvinylidene fluoride-hexafluoropropylene (PVDF-HFP) with a concentration of 3-4 wt%; the mass ratio of Au-PMO nanoreactor to PVDF-HFP is 1:2-3:10.
[0012] In this invention, in step (4), the spin coating speed is 400-600 rpm, and the spin coating speed has a certain influence on the thickness and flatness of the film; the vacuum drying temperature is 40-70℃, and the vacuum drying time is 6-18h.
[0013] In this invention, in step (4), heterojunction nanochannels with different film thicknesses can be obtained by controlling the mass ratio of the Au-PMO reactor, with the thickness varying between approximately 0.6-1.4 μm.
[0014] The present invention also provides a nanochannel based on a multi-level nanoreactor array prepared by the above preparation method, which is a heterogeneous and compact bilayer structure, with a porous anodic alumina (AAO) film layer as the lower layer and a periodic mesoporous organosilicon (PMO) nanoreactor array layer loaded with gold nanoparticles as the upper layer.
[0015] Compared with the prior art, the present invention has the following beneficial effects: This invention first prepares a sulfide-bridged periodic mesoporous organosilicon PMO, then loads gold nanoparticles (AuNPs) onto the PMO using an in-situ reduction method to construct a nanoreactor, Au-PMO. Subsequently, employing a multi-element assembly strategy, an Au-PMO layer is constructed on the surface of porous anodic alumina (AAO) using spin coating, ultimately obtaining an Au-PMO / AAO heterostructure film with both abundant mesoporous structure and tunable thickness. The uniformly sized and evenly distributed gold nanoparticles on the film enable it to exhibit peroxidase-like activity, providing a new material and approach for subsequent nanofluidic sensing, catalysis, and gating applications, paving the way for their promising future applications. Attached Figure Description
[0016] Figure 1 This is a transmission electron microscope (TEM) image of Au-PMOs in Example 1.
[0017] Figure 2 The image shows the X-ray diffraction (XRD) pattern of Au-PMOs in Example 1.
[0018] Figure 3This is a TEM image of Au-PMOs in Example 2.
[0019] Figure 4 The image shows the XRD pattern of Au-PMOs in Example 2.
[0020] Figure 5 This is a flowchart illustrating the fabrication process of the nanochannels in the multi-stage nanoreactor array based in Example 3.
[0021] Figure 6 This is a TEM image of Au-PMOs in Example 3.
[0022] Figure 7 The image shows the XRD pattern of Au-PMOs in Example 3.
[0023] Figure 8 The image shows the UV-Vis absorption spectra of Au-PMOs and PMOs in Example 3.
[0024] Figure 9 The image shows the UV-Vis absorption spectrum of the colorimetric reaction of TMB in Example 3.
[0025] Figure 10 This is a cross-sectional scanning electron microscope (SEM) image of Au-PMO / AAO in Example 3.
[0026] Figure 11 The nitrogen adsorption-desorption curve (a) and pore size distribution curve (b) of Au-PMOs in Example 3 are shown.
[0027] Figure 12 The X-ray photoelectron spectra of AAO and Au-PMO / AAO in Example 3 are shown.
[0028] Figure 13 The current stability of Au-PMO / AAO in Example 3. Detailed Implementation
[0029] The present invention will now be described in detail with reference to the accompanying drawings and specific embodiments.
[0030] Example 1
[0031] 1) First, thioether-bridged periodic mesoporous organosilicon PMO nanoparticles with a shell-core structure were prepared by a one-step hydrothermal synthesis method; wherein, step 1) specifically includes the following steps: Step 1-1: Prepare the template solution by adding 0.16g of cetyltrimethylammonium bromide (CTAB) to an ethanol-water solution containing ammonia and stirring the mixture at 35°C. The ethanol-water solution containing ammonia is prepared by mixing 1mL of ammonia, 30mL of ethanol, and 75mL of deionized water. Step 1-2: Prepare a mixed solution of bis[3-(triethoxysilyl)propyl]tetrasulfide (TESPTS) (0.1 mL) and tetraethyl orthosilicate (TEOS) (0.25 mL), and add it to the solution obtained in step 1-1 under vigorous stirring. Stir at 35°C for 24 h. Steps 1-3 involve centrifuging and washing the mixture obtained in Steps 1-2 to obtain the nanosphere product. Figure 2 TEM image of the nanosphere product; Steps 1-4: Disperse the nanospheres obtained in Steps 1-3 in 30 mL of deionized water and transfer them to a hydrothermal reactor, where they are reacted at 150 °C for 12 h. Steps 1-5: After centrifugation and washing of the mixture obtained in steps 1-4, the hydrothermal shell-core structured nanoparticle product is obtained. Steps 1-6: Disperse the nanoparticle product obtained in steps 1-5 in 120 mL of hydrochloric acid-ethanol solution, heat at 60 °C for 3 h, repeat this step three times to remove the template agent; the hydrochloric acid-ethanol solution is prepared by mixing 37% concentrated hydrochloric acid and ethanol at a volume ratio of 1:500. Steps 1-7: Centrifuge and dry the mixture obtained in steps 1-6 to obtain sulfide-bridged shell-core structured periodic mesoporous organosilicon nanoparticles (PMOs).
[0032] 2) The prepared PMO particles were dispersed in 20 mL of water at a concentration of 2 mg / mL, and 25 mL of 2.4 mM HAuCl4 solution was added. The pH of the solution was then adjusted to 7-8 with 1 M NaOH solution for in-situ reduction experiments. The mixture was stirred continuously at room temperature for 24 h. The resulting mixed solution was centrifuged and washed to obtain a mesoporous organosilicon Au-PMO nanoreactor loaded with gold nanoparticles.
[0033] like Figure 1 The image shown is a TEM image of Au-PMOs, which are loaded with gold nanoparticles with an average size of about 15 nm.
[0034] like Figure 2 The XRD pattern of Au-PMOs is shown, exhibiting typical gold (PDF card number 04-0784) diffraction peaks.
[0035] Example 2
[0036] 1) First, thioether-bridged periodic mesoporous organosilicon PMO nanoparticles with a shell-core structure were prepared by a one-step hydrothermal synthesis method; wherein, step 1) specifically includes the following steps: Step 1-1: Prepare the template solution by adding 0.16g of cetyltrimethylammonium bromide (CTAB) to an ethanol-water solution containing ammonia and stirring the mixture at 35°C. The ethanol-water solution containing ammonia is prepared by mixing 1mL of ammonia, 30mL of ethanol, and 75mL of deionized water. Step 1-2: Prepare a mixed solution of bis[3-(triethoxysilyl)propyl]tetrasulfide (TESPTS) (0.1 mL) and tetraethyl orthosilicate (TEOS) (0.25 mL), and add it to the solution obtained in step 1-1 under vigorous stirring. Stir at 35°C for 24 h. Steps 1-3: After centrifugation and washing of the mixture obtained in Steps 1-2, nanospheres are obtained. Steps 1-4: Disperse the nanospheres obtained in Steps 1-3 in 30 mL of deionized water and transfer them to a hydrothermal reactor, where they are reacted at 150 °C for 12 h. Steps 1-5: After centrifugation and washing of the mixture obtained in steps 1-4, the hydrothermal shell-core structured nanoparticle product is obtained. Steps 1-6: Disperse the nanoparticle product obtained in steps 1-5 in 120 mL of hydrochloric acid-ethanol solution, heat at 60 °C for 3 h, repeat this step three times to remove the template agent; the hydrochloric acid-ethanol solution is prepared by mixing 37% concentrated hydrochloric acid and ethanol at a volume ratio of 1:500. Steps 1-7: Centrifuge and dry the mixture obtained in steps 1-6 to obtain sulfide-bridged shell-core structured periodic mesoporous organosilicon nanoparticles (PMOs).
[0037] 2) The prepared PMO particles were dispersed in 20 mL of water at a concentration of 2 mg / mL, and 25 mL of 3.6 mM HAuCl4 solution was added. The pH of the solution was then adjusted to 7-8 with 1 M NaOH solution for in-situ reduction experiments. The mixture was stirred continuously at room temperature for 24 h. The resulting mixture was centrifuged and washed to obtain a mesoporous organosilicon Au-PMO nanoreactor loaded with gold nanoparticles.
[0038] like Figure 3 The image shown is a TEM image of Au-PMOs, which are loaded with gold nanoparticles with an average size of about 8 nm.
[0039] like Figure 4 The XRD pattern of Au-PMOs is shown, exhibiting typical gold (PDF card number 04-0784) diffraction peaks.
[0040] Example 3
[0041] like Figure 5As shown, a method for preparing nanochannels based on a multi-level nanoreactor array includes the following steps: 1) First, thioether-bridged periodic mesoporous organosilicon PMO nanoparticles with a shell-core structure were prepared by a one-step hydrothermal synthesis method; wherein, step 1) specifically includes the following steps: Step 1-1: Prepare the template solution by adding 0.16g of cetyltrimethylammonium bromide (CTAB) to an ethanol-water solution containing ammonia and stirring the mixture at 35°C. The ethanol-water solution containing ammonia is prepared by mixing 1mL of ammonia, 30mL of ethanol, and 75mL of deionized water. Step 1-2: Prepare a mixed solution of bis[3-(triethoxysilyl)propyl]tetrasulfide (TESPTS) (0.1 mL) and tetraethyl orthosilicate (TEOS) (0.25 mL), and add it to the solution obtained in step 1-1 under vigorous stirring. Stir at 35°C for 24 h. Steps 1-3 involve centrifuging and washing the mixture obtained in Steps 1-2 to obtain the nanosphere product. Figure 2 TEM image of the nanosphere product; Steps 1-4: Disperse the nanospheres obtained in Steps 1-3 in 30 mL of deionized water and transfer them to a hydrothermal reactor, where they are reacted at 150 °C for 12 h. Steps 1-5: After centrifugation and washing of the mixture obtained in steps 1-4, the hydrothermal shell-core structured nanoparticle product is obtained. Steps 1-6: Disperse the nanoparticle product obtained in steps 1-5 in 120 mL of hydrochloric acid-ethanol solution, heat at 60 °C for 3 h, repeat this step three times to remove the template agent; the hydrochloric acid-ethanol solution is prepared by mixing 37% concentrated hydrochloric acid and ethanol at a volume ratio of 1:500. Steps 1-7: Centrifuge and dry the mixture obtained in steps 1-6 to obtain sulfide-bridged shell-core structured periodic mesoporous organosilicon nanoparticles (PMOs).
[0042] 2) The prepared PMO particles were dispersed in 20 mL of water at a concentration of 2 mg / mL, and 25 mL of 4.8 mM HAuCl4 solution was added. The pH of the solution was then adjusted to 7-8 with 1 M NaOH solution for in-situ reduction experiments. The mixture was stirred continuously at room temperature for 24 h. The resulting mixed solution was centrifuged and washed to obtain a mesoporous organosilicon Au-PMO nanoreactor loaded with gold nanoparticles.
[0043] like Figure 6The image shown is a TEM image of Au-PMOs. After hydrothermal treatment, PMOs underwent structural transformation to form a unique core-shell structure. Gold nanoparticles were loaded onto the PMOs through in-situ reduction, and the gold nanoparticles were uniformly dispersed in the PMOs with an average size of about 5 nm.
[0044] like Figure 7 The XRD pattern of Au-PMOs is shown, exhibiting typical gold (PDF card number 04-0784) diffraction peaks.
[0045] like Figure 8 The UV-Vis absorption spectra of Au-PMOs and PMOs are shown. The Au-PMOs dispersion shows a distinct red color and exhibits strong absorption at 512 nm, which is attributed to the plasmon resonance band of AuNPs.
[0046] like Figure 9 The image shows the UV-Vis absorption spectrum of the colorimetric reaction of TMB in the system. It can be seen that only Au-PMOs in the presence of H₂O₂ can significantly catalyze the oxidation of TMB, exhibiting a distinct absorption peak at 654 nm. This indicates that Au-PMOs possess certain peroxidase-like activity, capable of catalyzing the decomposition of H₂O₂ into hydroxyl radicals, which subsequently oxidize TMB to produce the colorimetric reaction.
[0047] 3) The mesoporous organosilicon Au-PMO nanoparticles loaded with gold nanoparticles were dispersed in an acetone solution containing (3.8 wt%) PVDF-HFP by ultrasound. The mass ratio of PMOs to PVDF-HPF was 9:20. The mixture was sonicated for 2 hours to obtain a uniform suspension.
[0048] 4) Spin-coating the dispersion onto a porous anodic aluminum oxide (AAO) membrane (500 rpm), followed by vacuum drying at 60 °C for 12 h to obtain a composite nanochannel film.
[0049] In this embodiment, an Au-PMO / AAO nanochannel was prepared. The nanochannel includes a one-dimensional anodic aluminum oxide nanochannel with a thickness of about 60 μm and an average pore size of about 20 nm, and an Au-PMO layer with a thickness of about 1.0 μm.
[0050] like Figure 10 The image shows a SEM image of Au-PMO / AAO. As can be seen from the image, Au-PMOs are closely located on top of AAO.
[0051] like Figure 11 The figure shows the nitrogen adsorption-desorption curve (a) and pore size distribution curve (b) of Au-PMOs. As can be seen from the figure, PMOs have a high specific surface area and an average pore size of about 2.6 nm.
[0052] like Figure 12 The X-ray photoelectron spectra of AAO and Au-PMO / AAO are shown. As can be seen from the figure, after introducing the Au-PMO layer, new characteristic peaks of Au 4d, Au 4f, S 2p and Si 2p appear in the XPS spectrum.
[0053] Subsequently, electrochemical tests were performed on the prepared Au-PMO / AAO thin film. The film was placed between two conductivity cells, and electrochemical workstations were used to test the film at different concentrations (10... -2 M, 10 -3 M, 10 -4 Current stability in M)KCl solution.
[0054] like Figure 13 As shown, when +1V and -1V voltages are applied cyclically, the transmembrane ion current of the thin film is relatively stable with no obvious decay, and the current increases with the increase of KCl concentration, indicating that Au-PMO / AAO can be tested for a long time and has good current stability.
[0055] These results demonstrate that this embodiment successfully prepared Au-PMO / AAO films with abundant mesoporous channels and peroxidase-like activity.
[0056] In summary, the multi-level nanoreactor array-based nanochannel fabrication method of this embodiment successfully prepared Au-PMO / AAO films with abundant mesoporous channels and peroxidase-like activity, providing a new material for intelligent nanofluidic nanochannel devices in the fields of sensing, gating, and catalysis.
Claims
1. A method for preparing nanochannels based on a multi-level nanoreactor array, characterized in that, The specific steps are as follows: (1) First, thioether-bridged periodic mesoporous organosilicon PMO nanoparticles with a shell-core structure were prepared by a one-step hydrothermal synthesis method. (2) The prepared PMO particles were dispersed in water, and a certain concentration of HAuCl4 solution was added. Then the pH of the solution was adjusted to carry out an in-situ reduction experiment. After the reaction was completed, the mixed solution was centrifuged and washed to obtain a mesoporous organosilicon Au-PMO nanoreactor loaded with gold nanoparticles. (3) Disperse the mesoporous organosilicon Au-PMO nanoparticles loaded with gold nanoparticles in an organic solution containing a binder to obtain a uniform dispersion. (4) Spin-coating the dispersion onto a porous anodic aluminum oxide (AAO) membrane and then vacuum drying to obtain a composite nanochannel film.
2. The method for preparing nanochannels based on a multi-stage nanoreactor array according to claim 1, characterized in that, In step (1), the specific preparation steps of the sulfide-bridged periodic mesoporous organosilicon PMO nanoparticles with a shell-core structure are as follows: ① Preparation of template agent solution: Add 0.1-0.2g of quaternary ammonium salt cationic surfactant cetyltrimethylammonium bromide (CTAB) to an ethanol aqueous solution containing ammonia, and stir at a temperature of 30℃-40℃; wherein: the ethanol aqueous solution containing ammonia is prepared by mixing 0.8-2mL of ammonia, 25-35mL of ethanol, and 70-80mL of deionized water; ② Mix 0.05-0.20 mL of bis[3-(triethoxysilyl)propyl]tetrasulfide (TESPTS) and 0.1-0.4 mL of tetraethyl orthosilicate (TEOS), and add the mixture to the solution obtained in step ① while stirring. Continue stirring at 30℃-40℃ for 20-30 h. ③ Centrifuge and wash the mixture obtained in step ② to obtain nanospheres; ④ Disperse the nanospheres obtained in step ③ in 20-40 mL of deionized water, and obtain shell-core structured nanoparticles by hydrothermal reaction, wherein the hydrothermal reaction temperature is 150℃-180℃ and the hydrothermal reaction time is 10-16 h; ⑤ By repeatedly washing the nanoparticles in a hydrochloric acid-ethanol solution at a temperature of 60℃-90℃ to remove the template agent, the shell-core structured thioether-bridged periodic mesoporous organosilicon PMO nanoparticles were finally obtained. The hydrochloric acid-ethanol solution was prepared by mixing concentrated hydrochloric acid and ethanol in a volume ratio of 1:450-1:
550.
3. The method for preparing nanochannels based on a multi-level nanoreactor array according to claim 1, characterized in that, In step (2), PMO particles are dispersed in water to obtain a PMO dispersion with a concentration of 1-5 mg / mL, the concentration of HAuCl4 solution is 2.4 mmol / L-4.8 mmol / L, and the volume ratio of HAuCl4 solution to PMO dispersion is 3:5-1:
1.
4. The method for preparing nanochannels based on a multi-stage nanoreactor array according to claim 1, characterized in that, In step (2), during the in-situ reduction experiment, the pH range of the solution was adjusted to 7-10 using 0.8-1.2 mol / L NaOH solution, and the mixture was stirred continuously at room temperature for 12-36 h; the size of the gold nanoparticles was between 4-20 nm; after the reaction was completed, the sample was washed with deionized water after centrifugation.
5. The method for preparing nanochannels based on a multi-stage nanoreactor array according to claim 1, characterized in that, In step (3), the Au-PMO nanoreactor is dispersed into an organic solution containing a binder under ultrasonic treatment; the ultrasonic treatment time is 1-3 hours.
6. The method for preparing nanochannels based on a multi-stage nanoreactor array according to claim 1, characterized in that, In step (3), the organic solution containing the binder is an acetone solution of polyvinylidene fluoride-hexafluoropropylene (PVDF-HFP) with a concentration of 3-4 wt%; the mass ratio of Au-PMO nanoreactor to PVDF-HFP is 1:2-3:
10.
7. The method for preparing nanochannels based on a multi-stage nanoreactor array according to claim 1, characterized in that, In step (4), the spin coating speed is 400-600 rpm; the vacuum drying temperature is 40-70℃; and the vacuum drying time is 6-18h.
8. The method for preparing nanochannels based on a multi-stage nanoreactor array according to claim 1, characterized in that, The thickness of the composite nanochannel film is between 0.6 and 1.4 μm.
9. A nanochannel based on a multi-level nanoreactor array prepared by the method according to claim 1, characterized in that, It has a heterogeneous and compact bilayer structure, with a porous anodic alumina (AAO) film layer on the bottom and a periodic mesoporous organosilicon (PMO) nanoreactor array layer loaded with gold nanoparticles on the top.