Novel streptomyces with broad-spectrum antifungal activity and application thereof

Abstract

The application discloses a novel Streptomyces with broad-spectrum antifungal activity and application thereof, and belongs to the field of agricultural microbial technology and biological control. The strain XJP35 is identified as a potential new species of Streptomyces, and is named as Streptomyces sp. XJP35. The strain has been preserved in the Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 64561. The Streptomyces disclosed by the application can efficiently and broadly inhibit the growth of various crop fungal disease pathogenic bacteria, and the strain can also produce siderophores. Therefore, the Streptomyces XJP35 disclosed by the application has important application potential in crop fungal disease prevention and promotion of crop iron absorption.

Description

Technical Field

[0001] This invention relates to the field of agricultural microbiology, and in particular to novel Streptomyces with broad-spectrum antifungal activity and their applications. Background Technology

[0002] Plant diseases are a major factor restricting the high quality and yield of crops. It is estimated that up to 20% of global food production is lost annually due to plant diseases. Fungal diseases account for approximately 70-80% of these losses. According to incomplete statistics, diseases caused by pathogenic fungi account for 16% of global crop losses. Because many pathogenic fungi can form spores with special structures or extremely strong resistance, they infect crops in various ways and can be spread through wind, rain, insects, and agricultural tools. Therefore, the damage caused by fungal diseases is becoming increasingly serious, and their spread is becoming increasingly widespread. To address the damage caused by fungal diseases, chemical control and crop breeding have become important control strategies. However, while chemical control is fast-acting, it easily induces drug resistance in pathogens and causes irreparable environmental damage; while crop breeding requires a long cycle and is very difficult. Therefore, green and environmentally friendly biological control has become a research focus. Microbial control technology is highly efficient and pollution-free, and it meets the needs of green control and sustainable agricultural development.

[0003] Common fungal diseases of crops include wilt, downy mildew, anthracnose, root rot, leaf spot, Fusarium head blight, smut, sheath blight, and damping-off. Among these, *Fusarium oxysporum* can cause wilt in more than 100 plants, including cucurbits, solanaceous plants, bananas, and legumes, and is considered a globally widespread soil-borne pathogen. Banana wilt, caused by *Fusarium oxysporum* var. *cubicans*, has been dubbed "banana cancer," leading to a significant reduction in banana cultivation area. In my country's major banana-producing regions, this disease is showing a spreading trend. The pathogen of litchi downy mildew is *Peronophythora litchii*, which infects litchi leaves, flowers, and fruits, causing significant flower and fruit drop. It also occurs post-harvest, and is a major cause of low litchi yields and post-harvest rot, severely affecting litchi in major litchi-producing areas such as Guangdong and Guangxi in my country. Banana anthracnose is a fungal disease caused by Colletotrichum musae. It mainly affects immature or ripe bananas and is a serious disease during post-harvest storage. It causes black spots on bananas, severely affecting the appearance and quality of the fruit.

[0004] Currently, the types of microorganisms used for biocontrol include Trichoderma, Paecilomyces, Bacillus, Pseudomonas, and Actinomycetes. Among the reported antagonistic strains, Streptomyces antagonism is the most numerous. Streptomyces is known for producing abundant secondary metabolites; approximately two-thirds of the currently discovered bioactive natural products derived from microorganisms are antibacterial substances originating from Streptomyces. The secondary metabolites produced by Streptomyces possess antifungal, antibacterial, and anticancer effects, and have become an important source for discovering anti-active substances. Therefore, Streptomyces can be considered a good biocontrol agent. To further explore more biocontrol bacteria, it is urgent to screen new microbial resources with broad-spectrum resistance and high efficiency from the natural environment as novel biocontrol bacteria. Summary of the Invention

[0005] The purpose of this invention is to provide a new species of Streptomyces, XJP35, with broad-spectrum antifungal activity, providing a new microbial resource for the efficient control of tomato wilt, banana wilt, banana anthracnose, and litchi downy mildew.

[0006] To achieve the above objectives, this invention provides a novel Streptomyces XJP35 isolated from banana rhizosphere soil. This strain is deposited at the Guangdong Provincial Microbial Culture Collection Center (GDMCC), located at Building 59, No. 100 Xianlie Middle Road, Yuexiu District, Guangzhou, Guangdong Province, 510070, China, with accession number GDMCC No: 64561 and deposit date of April 25, 2024.

[0007] The identification characteristics of the Streptomyces mentioned above:

[0008] Strain XJP35 is Gram-positive. On TSA medium, its colonies are round, with pale yellow, smooth, opaque basal hyphae and irregular edges, while aerial hyphae are white. It can hydrolyze milk, Tween 20, and Tween 80, producing siderophores, but cannot hydrolyze starch or cellulose. The 16S rRNA gene sequence of strain XJP35 is 1517 bp in length, and phylogenetic analysis indicates that it belongs to the genus *Streptomyces*. Comparative genomic analysis shows that strain XJP35 has an average nucleotide identity of 84.95%–85.38% with the closest published *Streptomyces* type strains, and a simulated DNA hybridization value of 26.20%–28.00%, representing a new species of *Streptomyces*.

[0009] Therefore, the present invention provides the application of the above-mentioned Streptomyces XJP35, or its culture, bacterial liquid, fermentation broth, fermentation broth supernatant, or its fermentation extract as active ingredients in the preparation of microbial fertilizers.

[0010] This invention provides the application of Streptomyces XJP35, or its culture, bacterial suspension, fermentation broth, fermentation broth supernatant, or its fermentation extract as active ingredients in the preparation of pesticides for controlling plant pathogenic fungi.

[0011] Preferably, the plant pathogenic fungi include one or more of the following: banana wilt fungus, tomato wilt fungus, banana anthracnose fungus, and litchi downy mildew fungus.

[0012] The present invention also provides a microbial fertilizer or pesticide containing Streptomyces XJP35, or its culture, bacterial liquid, fermentation broth, fermentation broth supernatant, or its fermentation extract as active ingredients.

[0013] The present invention has the following advantages and effects compared with the prior art:

[0014] This invention reports for the first time a potential new species of Streptomyces, exhibiting significant control effects against Fusarium wilt of tomato, Fusarium wilt of banana, anthracnose, and downy mildew of litchi. The first discovery of this strain enriches my country's resources of beneficial microorganisms used for biocontrol and growth promotion. Its stable, efficient, and environmentally friendly antifungal effects make it a promising candidate for controlling fungal diseases in crops.

[0015] Streptomyces sp. XJP35 is deposited at the Guangdong Provincial Microbial Culture Collection Center (GDMCC), located at Building 59, No. 100 Xianlie Middle Road, Yuexiu District, Guangzhou, Guangdong Province, 510070, China. The accession number is GDMCCNo: 64561, and the deposit date is April 25, 2024. Attached Figure Description

[0016] Figure 1 The colony morphology of the novel Streptomyces XJP35 on R2A medium.

[0017] Figure 2 A phylogenetic tree of the novel Streptomyces XJP35.

[0018] Figure 3 The inhibitory effect of the novel Streptomyces XJP35 on various plant pathogenic fungi was studied.

[0019] Figure 4 Analysis of the growth-promoting characteristics of the novel Streptomyces XJP35 (A: protease assay, B: cellulase assay, C: siderophore production assay, D: hydrolysis of Tween 20, E: hydrolysis of Tween 80, F: IAA production assay).

[0020] Figure 5The inhibitory effect of the fermentation broth of novel Streptomyces XJP35 on Fusarium wilt of banana is shown in Figure A (left: morphological characteristics of Fusarium wilt of banana when co-cultured with novel Streptomyces XJP35; right: morphological characteristics of Fusarium wilt of banana when inoculated only with Fusarium wilt of banana; B: OD600 value of the bacterial broth when co-cultured with novel Streptomyces XJP35 and Fusarium wilt of banana; C: total number of colonies of Fusarium wilt of banana when co-cultured with novel Streptomyces XJP35 and Fusarium wilt of banana).

[0021] Figure 6 The study investigated the inhibitory effect of the fermentation extract of a novel Streptomyces strain XJP35 on Phytophthora downy mildew, the causal agent of litchi. Detailed Implementation

[0022] The present invention will be further described below with reference to the accompanying drawings and specific embodiments, but the embodiments do not limit the present invention in any way. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in this technical field.

[0023] Example 1: Isolation of rhizosphere bacteria from bananas

[0024] Rhizosphere soil from healthy banana plants in Machong Town, Dongguan City, Guangdong Province was collected and sieved through a 2mm sieve. The soil samples were then stored at 4℃. 10g of soil sample was weighed and added to an Erlenmeyer flask containing 90mL of sterile water. The flask was placed in a 28℃ constant-temperature shaker and incubated at 200rpm for 30min. A serial dilution method was used to obtain 10g of the sample. -4 10 -5 10 -6 100 μL of soil suspension was spread onto TSA medium (Qingdao Haibo) and incubated at 28°C for 7 days. Single colonies with different morphological characteristics were picked and transferred to fresh TSA medium for purification using the streak plating method. The purified strains were mixed with 25% glycerol (v / v) and stored at -80°C.

[0025] Example 2: Screening of strains antagonistic to Fusarium wilt of banana

[0026] The plate confrontation method was used to screen strains antagonistic to Fusarium wilt of banana. The specific method was as follows: A 5mm diameter mycelial cake of Fusarium wilt pathotype 4 was transferred to the center of a TSA plate. The tested banana rhizosphere strain was then inoculated around the center, 3cm away from the center. A negative control consisting only of the pathogen was used. All plates were co-cultured at 28℃ for 7 days, and the size of the inhibition zone was measured using calipers. Each strain was tested in triplicate. Based on the size of the inhibition zone, the strain XJP35, showing significant inhibitory effect, was selected for further analysis.

[0027] Example 3: Identification of antagonistic strain XJP35

[0028] Strain XJP35 was inoculated onto TSA medium and incubated at 28°C for 7 days for morphological observation. Colonies of strain XJP35 were white, round, dry, and opaque, with pale yellow substrate mycelium and white aerial hyphae. The colonies were embedded in the medium. Figure 1 ).

[0029] Genomic DNA was extracted from strain XJP35 using the HiPure bacterial DNA extraction kit (Guangzhou Meiji Biotechnology Co., Ltd.). Using the genomic DNA as a template, its 16S rRNA gene was amplified using universal bacterial primers 27F / 1492R. The PCR product was sent to Suzhou Genewiz Biotechnology Co., Ltd. for sequencing, and the sequence, shown in SEQ ID NO.1, is 1517 bp in length. Homology analysis of the sequenced data was performed on the EzBioCloud website. The results showed that strain XJP35 is homologous to the type strain *Streptomyces caatingaensis* CMAA1322. T It has the highest similarity at 99.17%; followed by Streptomyces hiroshimensis NBRC 3839. T (99.16%), Streptomyces ardus NBRC 13430 T (99.03%), and the 16S rRNA gene sequence similarity with other type strains of the genus Streptomyces was less than 99%. These analyses indicate that strain XJP35 is most closely related to the type strains Streptomyces caatingaensis and Streptomyces shiroshimensis. According to the description, 16S rRNA gene sequence alignment can only confirm that strain XJP35 belongs to the genus Streptomyces, but it is difficult to accurately determine its taxonomic position.

[0030] Example 4: Genome analysis of Streptomyces XJP35

[0031] The genome of strain XJP35 extracted in Example 3 was sent to Shanghai Meiji Biotechnology Co., Ltd. for genome sequencing using the Illumina Novaseq platform. Genome sequence assembly was performed using SPAdes v3.15.3 software, and low-quality contig sequences <500bp in length were removed. CheckM v1.1.3 software was used for quality control analysis of genome integrity and contamination. The total genome length of strain XJP35 was 7.76 Mbp, the N50 length was 98673 bp, the G+C content of the genomic DNA was 72.0%, the genome integrity was 98.5%, and the contamination rate was 3.3%.

[0032] A genome-based phylogenetic tree of strain XJP35 was constructed using UBCG v3.0 software. The results are as follows: Figure 2 As shown, strain XJP35 is similar to the model strain S. hiroshimensis JCM 4586 in genome-based evolutionary developmental analysis. T S. albireticuli NRRL B1670 T and S. eurocidicus ATCC 27428 T Belonging to the same branch indicates that strain XJP35 is related to the type strain S. hiroshimensis JCM 4586. T S. albireticuli NRRL B1670 T and S. eurocidicus ATCC 27428 T The closest kinship. Using online tools. http: / / www.ezbiocloud.net / tools / ani and https: / / ggdc.dsmz.de / ggdc.php# The tools were used to calculate the average nucleotide identity (ANI) and simulated DNA-DNA hybridization value (dDDH) between the genome sequences of strain XJP35 and the closely related model strain. The results are shown in Table 1. Strain XJP35 and the closely related model strain *S. hiroshimensis* JCM 4586... T S. albireticuli NRRL B1670 T and S. eurocidicus ATCC 27428 T The ANI values ​​were 84.95%, 85.15%, and 85.38%, respectively, and the dDDH values ​​were 26.20%, 26.80%, and 27.00%, respectively. Based on the internationally recognized thresholds of 95%-96% ANI and 70% dDDH for defining new species in prokaryotes, these results indicate that strain XJP35 is a potential new species of the genus *Streptomyces*.

[0033] Table 1 Comparative genomic analysis of strain XJP35 and its closely related type species

[0034]

[0035]

[0036] The XJP35 genome was functionally annotated using the online website antiSMASH. Results showed that strain XJP35 contains 68 gene clusters for secondary metabolite synthesis, including 19 PKS gene clusters, 12 NRPS gene clusters, 11 Terpene gene clusters, 9 PKS / NRPS gene clusters, 6 RiPP gene clusters, 3 NI-siderophore gene clusters, 2 Lantopeptide gene clusters, 2 Ampyccycl gene clusters, 2 Melanin gene clusters, 1 thioamitides gene cluster, and 1 indole gene cluster. Strain XJP35 contains several gene clusters that can produce antifungal and antibacterial secondary metabolites, showing 100% similarity to mediomycin A (antifungal activity) and minimycin (antibacterial activity). Simultaneously, three gene clusters related to siderophore synthesis were annotated in the genome of strain XJP35, one of which, a PKS / NRPS gene cluster, was annotated as the siderophore desferrioxamin B / E, with 100% similarity. In summary, this analysis indicates that strain XJP35 can produce both multiple antimicrobial species and siderophores. Therefore, strain XJP35 has significant application value in plant disease control and plant growth promotion. Strain XJP35 is named *Streptomyces p.* XJP35 and is deposited at the Guangdong Provincial Microbial Culture Collection Center (GDMCC), located at Building 59, No. 100 Xianlie Middle Road, Yuexiu District, Guangzhou, Guangdong Province, 510070, China, with accession number GDMCCNo: 64561, and deposited on April 25, 2024.

[0037] Example 5: Determination of the broad-spectrum antifungal activity of the novel Streptomyces XJP35

[0038] As in Example 2, the antagonistic fungal activity of strain XJP35 was detected using the plate confrontation method. The pathogenic fungi used in the experiment included Physiological Race 4 of Fusarium wilt of banana, Anthracnose of banana (Discocephala indicum), Fusarium wilt of tomato, and Phytophthora downy mildew of litchi.

[0039] As attached Figure 3As shown, strain XJP35 significantly inhibited the growth of four different plant pathogenic fungi tested, with inhibition efficiencies exceeding 50% for all four. It exhibited a particularly strong inhibitory effect on the mycelium of *Phytophthora licheniformis*, with an inhibition rate as high as 77.14%. *Fusarium wiltii* of banana and *Fusarium wiltii* of tomato could not expand and form a clear inhibition zone around strain XJP35, with inhibition rates of 62.01% and 61.82%, respectively. Strain XJP35 showed a slightly weaker inhibitory effect on *Anthracnose fungus* of banana, significantly inhibiting the growth of basal mycelium while having little effect on aerial mycelium, with an inhibition rate of 50.07%. These results indicate that strain XJP35 has a significant inhibitory effect on the growth of *Fusarium wiltii* of banana, *Anthracnose fungus* of banana, *Phytophthora licheniformis* of litchi, and *Fusarium wiltii* of tomato.

[0040] Table 2. Results of the determination of antagonistic pathogenic fungi of strain XJP35

[0041]

[0042] Note: Different lowercase letters after the data in the table indicate significant differences at the p<0.05 level.

[0043] Example 5: Determination of disease resistance and growth-promoting characteristics of a novel Streptomyces XJP35

[0044] Extracellular enzyme detection: 1% soluble starch, 10% sterilized milk, 1% sodium carboxymethyl cellulose, 1% Tween 20, and 1% Tween 80 were added to TSA medium, respectively. Strain XJP35 was inoculated onto these extracellular enzyme detection media and all plates were incubated at 28℃ for 3-7 days to observe extracellular enzyme production. For amylase detection, the medium was covered with Lur's iodine solution, and the presence of a clear hydrolysis zone was observed. The appearance of a clear zone indicates that the strain can produce amylase. The appearance of a clear zone on the plate with sterilized milk indicates that the strain can produce protease. For cellulase detection, the medium was stained with 1 g / L Congo red solution for 30 min, the stain was removed, and the medium was destained with 1 mol / L NaCl solution for 30 min. The appearance of a clear zone around the colony indicates that the strain can produce cellulase. On plates with Tween 20 and 80, the appearance of snowflake-like halos around the colonies indicates that the strain can produce esterase.

[0045] Test of the ability of the strain to produce siderophores: The strain XJP35 was inoculated on a TSA plate and incubated at 28°C for 3 days. Then, CAS medium containing 0.9% agar was used to cover the entire plate and incubated at 28°C for 4 days. After that, the color around the colony was observed to change from blue to yellow. If a yellow halo appeared, it indicated that the strain could produce siderophores.

[0046] IAA production capacity assay: 0.2 g / L L-tryptophan was added to LB liquid medium. Strain XJP35 was transferred to LB liquid medium and cultured at 28℃ with shaking at 180 rpm for 2 days. After centrifugation at 10000 rpm for 10 min, 200 μL of the supernatant was added to a 1.5 mL EP tube, and an equal volume of Salkowski colorimetric solution was added at a 1:1 volume ratio. LB medium without bacterial culture was used as a control, and three replicates were designed. After incubating the mixture at room temperature in the dark for 30 min, the solution was compared with the control (CK). A pink color indicated that the strain could produce IAA.

[0047] The results show that ( Figure 4 The strain XJP35 can hydrolyze milk at Tween 20 and 80, producing siderophores; however, it cannot hydrolyze starch and does not produce IAA. This indicates that strain XJP35 has significant application potential in promoting iron absorption in plants.

[0048] Example 6: Inhibitory effect of fermentation supernatant of strain XJP35 on spores of Fusarium wilt of banana

[0049] Strain XJP350 was transferred to 10 mL of TSB liquid medium and cultured at 30°C with shaking at 180 rpm for 2 days. After centrifugation at 10,000 rpm for 5 min, the fermentation supernatant was collected and filtered through a 0.22 μm filter membrane. The banana wilt pathogen was transferred to 100 mL of PDB medium and cultured at 28°C with shaking at 180 rpm for 2 days. The spores were then collected by filtration through gauze and centrifugation. The spores were resuspended in physiological saline, and the spore count was performed using a hemocytometer. First, 1 mL of PDB liquid medium was added to a 24-well plate, followed by 0.3 mL of the fermentation supernatant of strain XJP35, and then banana wilt pathogen spores were added to bring the final concentration to 1.0 × 10⁻⁶. 5 1 spore / mL. A negative control was prepared using TSB medium alone. Each treatment was repeated in triplicate. The OD of the mixed bacterial culture was measured after incubating the 24-well plate at 28°C for 24 hours. 600 The morphology of the pathogenic fungal hyphae was observed under a microscope. Simultaneously, 1 mL of co-culture and control bacterial suspensions were serially diluted, and 10 mL of each was used as the final product. -3 10 -4 10 -5 100 μL of the diluted solution was spread on PDA medium, with three replicates for each dilution. After incubation at 28°C for 2 days, the number of banana wilt pathogen colonies was counted.

[0050] like Figure 5 As shown, compared with the control group which was only inoculated with spores of Fusarium wilt of banana, the growth of Fusarium wilt of banana treated with fermentation broth of strain XJP35 was significantly inhibited, and its OD... 600 The colony count was significantly lower than that of the control (CK) treatment, at 5.52 × 10⁻⁶.4 The CFU / mL level was significantly lower than the 7.93 × 10⁻⁶ CFU / mL level in the control treatment. 5 CFU / mL. Microscopic observation revealed a significant reduction in the number of mycelia of *Fusarium wilt* var. *banana* treated with fermentation broth from strain XJP35, with altered mycelial morphology, characterized by shorter and more branched hyphae. These results indicate that the fermentation broth of strain XJP35 can significantly inhibit the growth of *Fusarium wilt* var. *banana*.

[0051] Example 7: Inhibitory effect of fermentation extract of strain XJP35 on Phytophthora downy mildew of litchi.

[0052] Strain XJP35 was transferred to 10 mL of TSB and 200 mL of ISP liquid medium, respectively, and cultured at 30 °C with shaking at 180 rpm for 6 days. The culture was then filtered through cotton swabs, the bacterial cells were discarded, and the fermentation broth was extracted 2-3 times sequentially with 200 mL of ethyl acetate and n-butanol, respectively. The extracts were then evaporated to dryness under vacuum to obtain the fermentation extracts. Each extract was dissolved in DMSO to prepare test samples with a concentration of 20 mg / mL for later use.

[0053] The inhibitory effect of fermentation extracts on Phytophthora downy mildew of litchi was evaluated using the plate perforation method. Figure 6 As shown, *Phytophthora indicum*, the causal agent of litchi downy mildew, was first inoculated in the center of an R2A plate. Then, 200 μL of different fermentation extracts were sequentially added to the pre-drilled R2A plates, including total TSB extract, total ISP extract, TSB-EA (ethyl acetate) extract, and TSB-Bu (n-butanol) extract. Equal volumes of DMSO solvent were added to the four wells of the blank control, and the plates were incubated at 30°C for 7 days before observation. Figure 6 As shown, compared with the blank control with only DMSO added, the growth of Phytophthora litatifolia, the causal agent of litchi downy mildew, was significantly inhibited when treated with the fermentation extract of strain XJP35.

[0054] The above results indicate that strain XJP35 (Streptomyces sp.) is a potential new species of the genus Streptomyces, capable of significantly inhibiting the growth of Fusarium wilt of banana, Phytophthora downy mildew of litchi, Phytophthora anthracnose of banana, and Fusarium wilt of tomato. It is a broad-spectrum and highly effective biocontrol strain, suitable for the production of microbial fertilizers, pesticides, and microbial agents, and subsequently applied to the control of the aforementioned crop diseases. Furthermore, strain XJP35 can produce siderophores, thus possessing significant potential for promoting iron uptake in crops.

[0055] 16S rRNA gene sequence of strain XJP35 (SEQ ID NO.1)

[0056] TTCACGGAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG

[0057] CAAGTCGAACGATGAACCCGCTTCGGTGGGGGATTAGTGGCGAACGGGTGAGTAAC

[0058] ACGTGGGCAATCTGCCCTGCACTCTGGGACAAGCCCTGGAAACGGGGTCTAATACCG

[0059] GATACGACCTGCCGAGGCATCTTGGCGGGTGGAAAGCTCCGGCGGTGCAGGATGAG

[0060] CCCGCGGCCTATCAGCTTGTTGGTGGGGTGATGGCCTACCAAGGCGACGACGGGTAG

[0061] CCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTAC

[0062] GGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCC

[0063] GCGTGAGGGATGACGGCCTTCGGGTTGTAAACCTCTTTCAGCAGGGAAGAAGCGCAA

[0064] GTGACGGTACCTGCAGAAGAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTAAT

[0065] ACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTT

[0066] GTCGCGTCGGATGTGAAAGCCCGGGGCTTAACCCCGGGTCTGCATTCGATACGGGCA

[0067] GGCTAGAGTTCGGTAGGGGAGATCGGAATTCCTGGTGTAGCGGTGAAATGCGCAGAT

[0068] ATCAGGAGGAACACCGGTGGCGAAGGCGGATCTCTGGGCCGATACTGACGCTGAGG

[0069] AGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAAC

[0070] GTTGGGCACTAGGTGTGGGCACATTCCACGTGGTCCGTGCCGCAGCTAACGCATTA

[0071] AGTGCCCCGCCTGGGAGTACGGCCGCAAGGCTAAAACTCAAAGGAATTGACGGGG

[0072] GCCCGCACAAGCAGCGGAGCATGTGGCTTAATTCGACGCAACGCGAAGAACCTTACC

[0073] AAGGCTTGACATACACCGGAAACGGCCAGAGATGGTCGCCCCCTTGTGGTCGGTGTA

[0074] CAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCCGCA

[0075] ACGAGCGCAACCCTTGTTCTGTGTTGCCAGCATGCCTTTCGGGGTGATGGGGACTCAC

[0076] AGGAGACTGCCGGGGTCAACTCGGAGGAAGGTGGGGACGACGTCAAGTCATCATGC

[0077] CCCTTATGTCTTGGGCTGCACACGTGCTACAATGGCCGGTACAATGAGCTGCGATAC

[0078] CGTGAGGTGGAGCGAATCTCAAAAAGCCGGTCTCAGTTCGGATTGGGGTCTGCAACT

[0079] CGACCCCATGAAGTTGGAGTTGCTAGTAATCGCAGATCAGCATTGCTGCGGTGAATA

[0080] CGTTCCCGGGCCTTGTACACACCGCCGTCACGTCACGAAAGTCGGTAACACCCGAA

[0081] GCCGGTGGCCCAACCCTTGTGGAGGGAGCCGTCGAAGGTGGGACTGGCGATTGGGA

[0082] CGAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATCACCTCCTTT

Claims

1. Streptomyces sp. XJP35, accession number: GDMCC No:64561.

2. The use of Streptomyces XJP35 as described in claim 1, or its culture, bacterial liquid, fermentation broth, fermentation broth supernatant, or fermentation extract as an active ingredient in the preparation of microbial fertilizers.

3. The use of Streptomyces XJP35 as described in claim 1, or its culture, bacterial liquid, fermentation broth, fermentation broth supernatant, or fermentation extract as an active ingredient in the preparation of pesticides for controlling plant pathogenic fungi.

4. The application according to claim 3, characterized in that, The plant pathogenic fungi mentioned include one or more of the following: banana wilt fungus, tomato wilt fungus, banana anthracnose fungus, and litchi downy mildew fungus.

5. A microbial fertilizer or pesticide, characterized in that, The active ingredient is Streptomyces XJP35 as described in claim 1, or its culture, bacterial liquid, fermentation broth, fermentation broth supernatant, or its fermentation extract.

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